Document G6NqVOD24q67xLKjMgRQe8Eex

AR 2 6 _ 021 ACUTE TOXICITY TO FISH (BLUEGILL) TEST SUBSTANCE_______________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-600. Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Not noted. Test type: Static acute GLP: No Year Completed: 1975 Species: Lepomis macrochirus Supplier: Not noted. Analytical monitoring: Temperature, pH, DO Exposure period: 96-hours Statistical methods: Probit analysis. Test fish age: Juveniles Average Length and weight: Length = 1 inch Weight = 0.5 grams Loading: Not noted. Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered water, City of St. Paul, MN Dilution water chemistry: Not noted. Lighting: Not noted Stock and test solution preparation: Test solutions were created by direct weights additions. Concentrations dosing rate: Once Stability of the test chemical solution: Not noted Exposure vessels: Not noted. Number of replicates: One Number of fish per replicate: 20 001998 Number of concentrations: 5 plus a blank control Water chemistry during the study: pH range (0 - 96 hours): 7.1 - 7.2 (control exposures) 7.2 - 7.3 (2,000 mg/L exposure)* Dissolved Oxygen range (0 - 96 hours): 5.6 - 7.0 mg/L (control exposures) 4.5 - 6.0 mg/L (2,000 mg/L exposure)* Temperature (0 - 96 hours): 72F * Values for the 2,000 mg/L exposure (third highest concentration) were used because total mortality occurred in the highest concentrations tested. RESULTS______________________________ =____________________ Nominal concentrations: Blank control, 1,000,1,500, 2,000, 3,000, and 4,000 mg/L Element value: 96-hour LC50 = 1,500 (1,282 - 1,755) mg/L Element value based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. CONCLUSIONS____________________________________ :__________ The FC-600 96-hour LC50for Lepomis macrochirus was determined to be 1,500 mg/L with a 95% confidence interval of 1,282 to 1,755 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking 3. The study lacks documentation on methodology. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solutions. REFERENCES---------------------------------------------------------------------------------------- This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, 1975. OTHER_______________________________________________ Last changed: 6/28/00 00?S 99 740101 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET Typ Test-- ` t e - H n v ir S-bati/' T . O rgani g ,W q i \ \ ________ Date Started IT M aterial Testeda F C -& S Ay Avg. Weight a 5 jt s tM I T j m L Date Completed T l*. Start d JQ ;t>w0 Pr// rft* P lo tte d LCca D ilu tio n Water f i Ite fe J - hy loo te r J jtp o tM s M tc to c k ftu s 24 h rs % D.O Control /0> t z to ihz&m ? / W u J & . \ s m E 100 1 1 a , o c o too p 6tP _ 4 jX 7 4 >57 4 WSL lo 7 6 5A Averase Teaneratui f - _ a 43 hrs % D.O 00 100 loo >5p 72 6 5 7 2 6 ,*i 7 a 54 ? ? 5 ,5 2 0 7 .3 72 hrs % D.O lo o ... 4*: T l &0 9q To m l o T i 5.4 4 o T .i 5 .0 0 T 3 4 .0 00 _____ 2 2 = ____ J L _______ 4 2 _____ _ tit- LCJ 0 C a lc u la tio n 93 h rs % D.O \w > t / 5 6 ^0 Z i 5 c S o 7.%4 , 4 4S o 7.S s 0 o 7 i- -- -H-+- 4-}4444t *s* <sOoB_> 0 10 20 30 BEST COPY AVAILABLE IDO , MiiLSenttlewLeMi environmental engineering laboratory AQUATIC TOXICITY - PROBIT ANALYSIS WORK SHEET b e s t c o p y a v a il a b Date S ta r te d : M ate rial T e ste d :______frC>-60O Time S t a r t e d : 2:oo (PM) T e st Organism jfnfbmd minmew) Type T e s t: (Coa tim io *--W 5 iO (S tatic) Avg. Wt. (gram) Avg. S iz e Exposure Period: Date Com pleted:___ ... D ilu e n t:(c a r b o n - filt.- S t.P . C ity Wtr.) A n alysis by: CD K No. o f Doses P lo tte d 1 2 3 4 Cone. 1 0 G oocT Observed % M ortality So 5o - -70 loo ( 2) C3) Expected % Corrected M ortality V alu es IS 5o 75 9o 9 6 .8 0-E ObservedExpected 7 $ 6.8 5 6 7 ^ (inch) C o n trib u tio n to chi(from Nomograph #11 .0 4 3 ---- ' ' 037 o. IS O Animals/Dose OO Total contribution to chi T o ta l Animals ______ _ (ch i2 ) c o n trib . to chi x Tot. Animals A ch i2 (P-.OS) fo r (K-2) 5 _______ d egree o f freedom (from ^Table 2) ^3 1 (=>Q> i s l e s s than S ^ ; t h e r e fo r e , the d a ta a r e not s i g n i f i c a n t l y heterogenous. 95% Confidence L im its f o r LCg* LC84 Slope SIS'o function (S) t i u j l l LC5C0rt IlC804,/'LC 50 + ^ s o ^ Sbo LC16 l 3l50^\Sc>c + ^QO/teSo * \At Ip S O M A As f T o ta l number o f an im als used between 16% and 84% exp ected e f f e c t s (N ")* fLC 50 (S) 2.77/1$' A4 (from Nomograph #2) .\ j a LC5c0n//fLC 50 lower lim it * \ 5 o Q / V I I LCS0 X fLCg0 * upper lim it \ 5 o o X \ A~\ \ , T 5 . . . V*w% I L LC50 (95% co n fid en ce lim it s to Al 1 J ,,5 5 j (1) Do n o t l i s t more than two c o n se c u tiv e 0% e f f e c t s o r more than two c o n se cu tiv e 100% e f f e c t s . (2) Expected v alu e fo r any dose sh ould be g r e a te r than 0.01% o r l e s s than 99.99%. (3) C o rrected v alu e fo r each 0% o r 100% e f f e c t (from T ab le 1 ). * There i s a 95% chance t h a t the tru e v a lu e o f L C ^ l i e s w ith in th e se l i m i t s . 002001 V .V O V f^ / ' i , ' A . 'IlL'jtlllili" 31QW1IWAW 1 J A ' i e n a