Document G6LKyOeKODq7gyaGBM9D3paon

M 9.SL6 - O73& TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203. Current information indicates it is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture wjth incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: NS-EN ISO 10253, 1998: Water quality - Marine algal growth inhibition test Type (test type): Acute Static GLP: No Year study performed: 2000 Species: Skeletonema costatum Source: Strain NIVA BAC1, stock culture maintained at RF - Rogaland Research. Element basis: Estimate biomass by measuring fluorescence. Exposure period: 72 hours Statistical Methods: Growth rates by log linear regression on biomass (fluorescence) vs. time, as described in NS-EN ISO 10253. Calculation of NOEC done by single factor ANOVA followed by Bonferronis procedure. Analytical monitoring: pH Test Conditions: Algal Nutrient Medium: Natural seawater (34 ppt salinity) from an unpolluted site in Byfjord, Norway, enriched with nutrients according to ISO 10253 and recommendations from the Paris Commission. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 8.3. Stock and test solution preparation: A 500.8 mg/L primary stock solution was prepared by diluting 0.5008 grams of test sample in 1 liter of growth medium. Aliquots were then added directly to growth medium in test vessels to create test solutions. 000752 Exposure vessels: 100 ml flat-bottomed flasks containing 50 ml test solution. Agitation: Shaken continuously on an orbital shaker. Number of replicates: 3 Initial cell loading: 2.0 x 103 cells/mL Number of concentrations: six plus a blank control Lighting: Continuous fluorescent lighting at >50 pE/m2 sek. Water Chemistry: pH range (0-96 hours): 8.3 - 8.4 (control exposure) 8.2 - 8.4 (100 mg/L exposure) Test temperature range: 20 2 C RESULTS________ :_____________________ *____________________ Nominal concentrations: Bk control, 0.32, 1,3.2, 10, 32, and 100 mg/L. Algal Growth Response ErC5ovalues (based on growth rate) 72-hour ErC50 = 62 (48 - 83) mg/L 72-hour NOEC = 32 mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not conducted. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ FC-203 exhibits a 72-hour ErC5o growth rate value of 62 mg/L with a 95% confidence interval of 48 -83 mg/L. The 72-hour No Observed Effect Concentration (NOEC) is 32 mg/L for growth rate. Validity criteria fulfilled; control cell density increased by a factor of more than 16 and the reference substance was within normal range. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study 000753 lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-2, 2000. OTHER______________________________________________________ Last changed: 6/27/00 000754 99312-2 NORSK AKKREDfTERING Nr.POIi ROGALAND RESEARCH "FC-203 Light Water Brand AFFF" Toxicity tests Report RF-2000/002 Lab. ref. no.: 99312-2 No. of pages: 31 ISBN: Author: Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 / 06.01 2000 Research Program: Open from (date): Summary: The following test results were obtained with "FC-203 Light Water Brand AFFF": Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h EC 48 h LC* 10 d LC* (mg/1): 62 (mg/l): 200 (mg/kg dry weight): >3060 The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this document may be reproduced in any form without permission in writing by RF - Rogaland Research <0. [Study director S Inger Lisa Andersen for RF - Rogaland Research Kre Netland 000755 i9a9O3.1r2e-j.2no.. r - uj j i CONTENTS: 1. Preface..................................................................................................................................................3 2. Te st fo r t o x ic it y w it h Sk e le to n e m a c o s ta t u m ............................ 4 3. Test for toxicity with A cartia tonsa........................... .7.............................................................10 4. T 15est for toxicity with Co r o p h iu m vo lu tato r ....................................................................... 5. Data sheet(s) .....................................................................................................................................20 000756 99312-2 O - PREFACE: Ecotoxicological tests wereperformed on "FC-203 Light WaterBrandAFFF"in agreement with Novatech. The sample is a concentrate that normally will be used in a 3% solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995 / SFT1998). The project way carried out at RF - Rogaland Researchfor 3M Norge. All the tests were performed by RF - Miljelab. Study director was Senior Engineer Inger Lisa Andersen. The test results are presented in separate sections. The results relate to theproduct as supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). IeTtqhauelispaomccsertnaettdeaistnatdthioawntotsrhtkeaitnleagsbptorhroaactteotdhrueyrhleaasbsfooarrastaaoltrlisytfhamecetteoetrssyt tmqhueetaNhloiAtdyrsaecsqosuunirrtaaeminnceeendstsiynscttoehnmec,aewcrnchriinecgdhictiaontmciolpuned.teesnce, internal quality control schemes, participation in interlaboratory comparisons, and the use of certified reference materials and secondary reference materials. The testsperformed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without thepermission in writing by RF - Miljolab. 000757 y y jy i-^ Test for T oxicity Marine alga: Skeletonema costatum 000758 99312-2 - K'J - * TEST REPORT aGlRgOaWTSHkIeNlHeItBoInTIeOmN aTEcSTosWtaITtHuTmHE MARINE (@ i NORSK AKKREDfTERING ROGALAND RESEARCH TEST SUBSTANCE: "FC-203 Light Water Brand AFFF" TEST RESULT: The 72 hours EC50 was calculated to 62 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: NwSit-hESNkeIlSeOton1e0m25a3c,o1s9ta9t8u:mWaantderPqhuaaeloitdya-cMtylaurminetraiclgoamlugtruomw.th inhibition test TEST ORGANISM: Skeletonema costatum, organism is kept at RF strain NIVA BAC 1. - Rogaland Research. A stock culture of the test PREPARATIONS: Nasabtuarsaisl sfeoarwthaetegrr(oswaltihnimtye:d3iu4m%.o)TfrhoemwaanteurnwpaosllfuiltteedresditethirnoBugyhfjoarGdFw/Casfuilsteerd, and briefly heated to about 75C prior to use. The seawater was enriched with nutrients according to ISO 10253, and recommendations from the Paris Commission. Tmmeges/d1tiuswomalus. tpiOornethpseawrreecdroenbcpyernsetptirraarrtieindognf0sr.ow5m0e0rae8spgtrorecapkmasrsoeodluffttrihooenmp. rtAhodissutscottcoikcnks1osloluiltutriteoiononfo.gfr5o0w0t.h8 INCUBATION: Tolaretbesitdtactluosltahubarokeuesrto,2f3050r0e0pmclielclawlstee/mrsefloifnrrcoeumabcahatnecdoenxinpceo1nn0ter0namttiiaollnfly.lagTt-rbeosowtttcionumgltueindroefscluawsluekrmseocinnuolatcnuure. The test was run under continuous light (> 50 pE/nr-sek) from fluorescentr " tubes of universal natural white type, at a temperature of 20 2C. The test was run for about 72 hours, and subsamples of 5 ml were taken from the test flasks each day for growth measurements. Algal growth was measured by fluorescence. pH measurements were performed as indicated on data sheet. 000759 L99a3b1.r2e-f2.no.: Page 6 o f 31 DATA ANALYSIS: CALCULATIONS: vGerroswusthtirmatee,saws edreesccrailbceudlaitnedNbSy-EloNgIlSinOea1r0r2e5g3r.ession on biomass (fluorescence) ErCso: The inhibitory effect on the growth of Skeletonema costatum is expressed as the concentration of a product giving 50% reduction of growth rate (ErCso). This effect concentration and the 95% confidence limit were calculated by the use of the computer programme TOXEDO developed by VKI (Denmark), assuming a logarithmic normal distribution of data. The programme also calculate concen trations inhibiting growth rate 10% and 90% (ErCio and ErC9o). NOEC: CfaacltcourlAatNioOn VofANfOolEloCw(eNdobOy bBsoenrfveerdroEnfifsepctroCcoendcuernetrfoarticoonm) ipsadroinngeebaychsingle concentration mean with the control mean. TEST RESULTS: Table 1. EC and NOEC values obtained with Skeletonema costatum. COMMENTS: VALIDATION: Test substance p o EAo EjCo "FC-203 Light Water Brand AFFF" 13 (8.3 - 18) 62 (48 - 83) >100 NOEC: 32 " iVVnaatelluurevessalaisnradericceaaitlnecducilocaantetcedednb.tyrNatthOieoEncCsogmairpveuintcegarl1pc0ur%olag,tre5ad0m%bmyaeunsTdinO9gX0tE%hDeiOcnoh(miVbpKituiIot,enDrpoernfogmgraorrawkm)th,marenadtGe.9ra5Up%hnPictosandinfiIdnmeSgnt/acl.et 3.0. The results are based on growth rates for the time span 0 - 72 h, the period d(suereinggrowwhtihchcutrhveegsr).owth is considered to be exponential in the control cultures The validity criteria given in the test protocol are fulfilled, in that the control c(ceollrrdeesnpsoitnydiinncgrteoasaegdrboywtahfraactteoroof fmmoroerethtahnan0,196din'1)t,htehteecsot npterroilodpH did not ___ vary by more than 1,0 units during the test and the 72 hours test results with 3,5-dichlorophenol was in the range normally found. Lab. ref. no.: Sample received.: Test period: Report date: 99312-2 20.09.99 04-07.10.99 06.01.2000 \SpAfV_ Elin Horve ge Molversmyr .. Engineer Senior Research Scientist Analytical technician Quality Assurance Enclosed: Data sheets Tfohrims toesthterrepthoarnt iisnisfusulle,dwbityhoaulat bthoeraptoerrymaiscsciroendiitnedwrbiytinNgobrwy eRgFia-nMAiclcjoreladbi.tation (NA). The report may not be reproduced in any 00(T lab.refno.: 312-2 Page 7 of 31 PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: 99312-2 Start date: 04.10.99 Cone, unit: mg/1 Measurement: The biomass was measured by fluorescence: values in fluorescence units Time: 04.10.99 05.10.99 06.10.99 07.10.99 Concentration 14:05 09:25 10:30 10:40 Growth pH pH rate Corre- at start at end day -1 lation of test of test 0 0.11 0.59 9.2 56 2.39 0.998 0 0.15 0.63 7.9 59 2.14 0.997 0 0.13 0.62 9 54 2.29 0.997 8.3 8.4 0 0.14 0.63 6.4 47 2.07 0.999 0 0.14 0.62 6.3 48 2.06 0.999 0 0.12 0.71 7.1 55 . 2.20 1.000 0.32 0.13 0.82 9.2 61 2.30 1.000 0.32 0.13 0.77 9.6 66 2.32 1.000 8.4 8.4 0.32 0.14 0.84 12.5 71 2.43 0.999 1 0.14 0.77 11 70 2.36 0.999 1 0.14 0.69 12 65 2.41 0.996 8.4 8.5 1 0.16 0.63 8.3 60 2.13 0.995 3.2 0.18 0.58 8 63 2.05 0.990 3.2 0.14 0.59 7.1 52 2.12 0.997 8.4 8.5 3.2 0.12 0.6 6.6 50 2.17 0.999 10 0.14 0.58 8.5 64 2.22 0.995 10 0.16 0.34 4.4 48 1.79 0.974 8.4 8.5 10 0.16 0.6 7.6 68 2.09 0.994 32 0.14 0.43 4.4 42 1.86 0.992 32 0.12 0.39 5.3 46 2.05 0.990 8.4 8.4 32 0.13 0.3 3.6 30 1.79 0.979 100 0.1 0.04 0.1 0.3 0.00 100 0.09 0.18 0.3 1.35 0.65 0.987 8.4 8.2 100 0.08 0.06 0.21 1.3 0.52 0.784 Reference substance (3,5-dichlorophenol): 1.5 mg/l 0.14 0.47 1.85 1.5 mg/I 0.16 0.43 1.45 1.5 mg/1 0.16 0.45 1.45 4.2 3.3 2.9 1.39 0.999 1.19 1.000 1.19 0.999 8.3 8.2 Mean values: 0 0.13 0.6 8 53 0.32 0.13 0.8 10 66 1 0.15 0.7 10 65 3.2 0.15 0.6 7 55 10 0.15 0.5 7 60 32 0.13 0.4 4 39 100 0.09 0.1 0 1 Reference subst. 0.15 0.45 1.6 3.5 2.19 2.35 2.30 2.11 2.03 1.90 0.39 1.26 0.998 1.000 0.997 0.996 0.991 0.988 0.917 1.000 The result of the reference substance is within the range 761QCnormally founQ l9a9b3.1r2e-f.2no.: Page 8 of31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: 99312-2 000762 l9a9b3.1r2e-f.2no.: Page Vof Si inhibition of the growth of Skeletonema costatum with: mg/1 (FC-203 Light Water Brand AFFF) E x p e rim e n ta l d a t a : Concentration in Growth rate FC-203 Light Water Brand AFFF Inhibition in percent Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean : 0.32 0.32 0.32 1.00 1.00 1.00 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 2.39 2.14 2.29 2.07 2.06 2.20 2.19 2.30 2.32 2.43 2.36 2.41 2.13 2.05 2.12 2.17 2.22 1.79 2.09 1.86 2.05 1.79 0.00 0.65 0.52 - 0 0.0 0.0 0.0 0.0 0.0 2.8 6.5 3.3 1.0 0.0 18.3 4.6 15.1 6.5 18.3 100.0 70.3 76.3 STATISTICAL PARAMETERS CALCULATED FROM CONTINUOUS RESPONSES 5 doses and 11 responses have been used during calculations. EC 10 : EC 50 : EC 90 : 13.33 61.95 288.00 95 % confidence limits : ( 95 % confidence limits : ( 95 % confidence limits : ( 8.29 .. 48.25 .. 186.95 .. Coefficient of determination :0.763 18.46) 83.10) 562.40) 000763 L99a3b1.r2e-f2.no.: Page 10 of 31 Test for T oxicity Marine herbivore: Acartia tonsa 000764 99L -" 31' 2-'J2 * ``o TEST REPORT MACAURTINEETCOOXPICEIPTOYDTAEScTdWrItTidH THE tOHSd (8 ) NORSK AKKREDffERING Nr.POI ROGALAND RESEARCH TEST SUBSTANCE: "FC-203 Light Water Brand AFFF" TEST RESULT: The 48 hours LC50 was calculated to 200 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ItoSOm/aDrIinSe1c4o6p6e9po- d1s99(C7:opWeaptoedraq,uCarliutsyta-cDeae)t.ermination of acute lethal toxicity TEST o r g a n i s m : Acartia tonsa University of (Dana), supplied Copenhagen. by the Marine B iological Laboratory, CULTIVATION: Twhitehttehset o"rPgaarnisisCmosmwmerisesisounpp-lRieidngasTeegsgt sP,raontodcwole"re(VcKulIt)i.vaLteadrgien caocpcoeprdoadnitcees and adults were used in the test. PREPARATIONS: Natural pasriborastios usfeosarew.thaetedri(lusatiloinnitwy:at3e4r.%To)hferowmatearnwunaps ofillltuetreeddstihteroinugBhyafjGorFd/Cwafisltuersed Tmweagst/1tersw.oalOusttpihorenerpscaworneedcreebnpytrresatptiiraorrniensdgwf0reo.r5me0p1a7restgporacarkmedssoforluof tmtihoenth.pirsAosdtsuotccoktckisnosl1oulltuiitotrineo.no fodf i1lu0t0i3on INCUBATION: T25hemtlesotfwteasst rsuonluitniognlaasnsdc5onatnaiimnearlss,(248rempllircoaltleesdfroirmeavciahlsc)oneaccehntcroantitoanin. iTnfgre vials were kept under a 16h/8h light/dark photoperiod at a temperature of 2d0ata s1heCet.. pH and oxygen measurements were performed as indicated on the 000765 L99ab3.1r2e-f2.no.: rage i z oj -w DATA ANALYSIS: CALCULATIONS: The effect on the survival ofAcartia tonsa is expressed as the concentration of paeprrtoedsutcctolnectehnatlrtaoti5o0n%weorfethuesetdesftoarnciamlcaulsla(tLioCn5.0)T. hTeh2e4cohmoubrisnaenddm4o8rthaoluitrys sLtCa5n0davradlupersocaenddutrheeP9R5%OBcIoTnAfidneanlycseisli,mNiat twioenrael cSawlceudlaisthedEbnyviurosinnmg etnhteal Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CfaacltcourlAatNioOn VofANfOolEloCw(eNdobOy bBsoenrfveerdroEnfifsepctroCcoendcuernetrfoarticoonm) ipsadroinnge ebaychsingle concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-203 Light Water Brand AFFF". Acartia tonsa 24 hours 48 hours LCto 160 (84-220) 76 (32-110) LC 50 340 (260-460) 200 (140-270) L C 90 740 (530-1400) 510 (350-1100) NOEC: 100 100 ctVhaaellcucueolsamitpnedudtibecryaptPerRo1gO0r%Ba,ImT5m0ae%naGlayrnsaidps,h9aP0%naddl9Ien5thS%atalcittoy3n.a0fi.fdteern2ce4 ianntedr4va8lhsoaurres.indUicnaittsedin. mNgO/lE. CVaarlueecsaalcreulated by VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end of the test was greater than or equal to 4 mg/l, the percentage mortality in the controls was less than 10%, and the 48 hours LC50 of 3,5-dichlorophenol (reference) was in the range normally found. Lab.ref.no.: Sample received.: TReesptoprterdiaotde:: 99312-2 20.09.99 0066-0.081.1.200.9090 AnalyEticnaglinteecehrnician SenQiouraRlietsyeAarscshurSacniceentist Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhoaulat bthoeraptoerrymiascsciroendiitnedwrbiytinNgobrwyeRgFia-nMAiclcjoreladbit.ation (NA). The report may not be reproduced in any 000766 l9a9b3.1r2c-j 2' ^ - - o- PRIMARY DATA: Acute toxicity test with Acartia tonsa Test substance: Lab. ref.: Start date: Measurement: "FC-203 Light Water Brand AFFF' 99312-2 06.10.99 Number of dead Concentration (mg/l) Control 10 32 100 320 1003 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 5 55 30 5 5 5 5 55 20 5 5 55 20 5 5 55 20 5 5 55 20 5 555 20 5 NUMBER OF DEAD 24 hours 48 hours ACE B DF Total AC E BDF Total 0 00 001 0 00 1 101 2 0 00 0 0 10 00 1 0 00 1 001 11 2 100 0 201 11 4- 2 12 3 44 2 7 3 14 5 55 5 55 5 20 5 20 Reference substance (3,5-dichlorophenol): 1.0 mg/l 5 55 020 254 30 5 55 1 00 3 130 The result with the reference substance is within the range normally found. 15 000767 99312-2 Test substance: FC-203 Light Water Brand AFFF" Lab. ref.: Start date: 99312-2 06.10.1999 Measurements of oxygen and pH. Concentration (mg/l) Start Oxygen (mg/l) pH 24 hours 4i hours Oxygen pH Oxygen (mg/l) (mg/l) Control 7.6 8.1 7.2 10 8.1 8.1 32 8.1 8.1 - 7.3 7.1 100 8.1 8.1 6.7 320 8.0 8.2 6.1 1003 7.3 8.2 6.6 8.1 pH 8.1 8.2 8.2 8.2 8.1 Reference substance (3,5-dichiorophenol): 1.0 mg/l 7.7 8.1 7.3 8.2 000768 T est for toxicity Marine Amphipode: Corophium volutator Lab.reJ.no.: 99312-2 Page 16 o f 31 TEST REPORT ACUTE TOXICITY marine amphipo SdEDCIMorEoNpThTEiuSTmWvIToHluTHtaEtor iO l NORSK AKKREDITERING Hi.POI ROGALAND RESEARCH TEST SUBSTANCE: "FC-203 Light Water Brand AFFF" TEST RESULT: The lOd LC50 was calculated to >3060 mg kg'1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ASTM E l367-90, Standard Guide For Conducting 10 day Static Sediment Toxicity Tests with Marine and Estuarine Sediments. TEST ORGANISM: Corophium volutator, from an unpolluted site near Trondheim. Cultivation: The test organisms were supplied as adults (4-8 mm in size) and were held in aerated seawater at 15C. A small amount o f natural seaweed was used for feeding. Preparation: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjorden outside wSantaaasvlyausnseegdderffoowrraopsrrugeaspenadircifncogorntdetiesltnustteiaodnnidmwpearntotespr..orTStiheodenismseidletnimftoceroneltlaeicschtaetdemsatu.tdadny usannpdolaluntdedwsaiste Each test concentration was prepared by adding the product in 600 grams of bduamckpetsewdaims sehnatkinenafporla3sthicoubrusc.keTth. e2s0e0dimmleonftsweaaws datievridwedasinatdodtehdreaendbetahkeers, 400 ml of seawater was added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample of tchaelcdualamtepdsaeds immgenktgw'1assedainmaleynsteddrfyorwweiagthetr. content, and all concentrations are INCUBATION: Tsceohdneicmteenestntrtwa, taaiopsnpr..u4nT0ih0nemgblealasoksfecsroesnawwtaeairnteeerrksaenp(6dt0u10n0dmaelnr)icmeoanaclthsin,cu3oonruetaspilaniecinraatgetis2o0fno0argteaaocfhtest temperature of 15 1C. pH and oxygen measurements were performed as indicated on the data sheet. 000770 99312-2 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Corophium volutator is expressed as the concen tration of a product lethal to 50% of the test animals (LCso)- The combined mortality per value and the test concentration were used for calculation. The 95% confidence limit are calculated by using the s1t0anddaayrdLC50 PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CfaacltcourlAatNioOn VofANfOolEloCw(eNdobOy bBsoenrfveerdroEnfifsepctroCcoendcuernetrfoarticoonm) ipsadroinngeebaychsingle concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-203 Light Water Brand AFFF" Corophium volutator 10 days LCio LCjo LC90 >3060 >3060 >3060 NOEC: 3060 GVVaaralluupeehssPainarddeiIccnaaStlectau1tl0a3%t.e0,d. 5b0y%PRanOdB9I0T%anlaeltyhsaisli.tyNaOfteErC1a0rdeacyas.lcuUlantitesdibnymthgekcgo'1msepduitmerepnrtodgrryawmemigeht. VALIDATION: Tothxheyegpveeanrlcicdeonitntyacgecenritmtreaortiriaotangliiavtytetnihneinthetnehdceootnefsttrthopelrstoewtsoetcrwoelalsaersgesrfetuhaaltfenirll1teh5da%,ninaonrthdeaqatuttahelsettodwi6sits0ho%lv,ed Fislufoouranndtetoneb(erwefietrheinncaec)cwepatseidnltihmeitrsanfogreonrogramniacllcyofnotuenndt.anTdhseilstefdriamcteinont .used Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0.8 LSaamb.prleef.rnecoe.:ived.: TReesptopretrdiaotde:: 2909.30192.9-92 03-13.12.99 06.01.2000 Elin Horve AnalytEicnaglinteecehrnician SeniAorgRe eMseoalvrcehrsSmcyierntist Quality Assurance Enclosed: Data sheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhoaultabthoeraptoerrmy iascsciorendiintewd rbiytinNgobrwy eRgFia-nMAiclcjoreladbit.ation (NA). The report may not be reproduced in any OOO 771 99312-2 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: Start date: Measurement: 99312-2 03.12.99 Number of dead Total number Number of specimen pr. vessel NUMBER OF DEAD 5 days 10 days Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F AC E B DF Total ACE BDF Total Control 10 10 10 60 10 10 10 - 000 000 0 10 10 10 91.1 30 2 00 2 10 10 10 501 30 101 2 1030 10 10 10 30 100 1 3060 10 10 10 30 102 3 Reference substance (fluoranthene): 10 10 10 20.3 30 3 22 The result with the reference substance is within the range normally found. 7 000772 L99ab3.1r2e-f2.no.: Page 19 o f31 Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: Start date: 99312-2 03.12.99 Measurements of oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 91 8.1 91.1 96 8.1 93 8.1 501 77 7.8 92 8.1 1030 97 8.1 93 8.1 3060 97 8.1 74 7.7 10 days Oxygen (%) pH 91 8.0 88 8.1 90 8.1 86 7.9 91 8.0 Reference substance (fluoranthene): 20.3 95 8.1 90 8.1 90 8.1 000773 L99a3b1.r2e-f2.no.: Page 20 of31 D ata Sheets 000774 * L99a3b1.r2e-f2.no.: Page 21 of31 HMS-DATA3LAD (Heise-, Milja- og Sikkerhetsdatablad) aaasssaaass: Dokumentnr.: Erstattet : 10-3814-0 Utgitt 03/11/98 Versjon 03/10/97 Skrevet ut : 11/11/98 Side ,, : 06.00 av KMS-databladet er utarbeidet av Miljeavdelingen ved 3M Norge A/S, Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIFIKASJON AV KJEMIKALIET OG ANSVARLIG FIRMA. Importar: 3M Norge A/S Kandelsnavn: FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Deklaras jor.snummer, Produktregisteret: P-nr.: 002614. Telefon, Gift info m a s jor.ssentralen: 22 59 13 00 2 OPPLYSNINGER OM KJEMISK SAMMSNSETNING. Informasjon om enkeltkomponenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VKT-PROSENT VANN 7732-18-5 2-(2-3UTOKSYETOKSY)-ETANOL / 112-34-5 (DIETYLENGLYK0LM0N03UTYLETER ) Faresymboler/risikosetninger: Xi; R: 36 1,2-ETANDIOL 107-21-1 Faresymboler/risikosetninger: Xn; R: 22 SYNTETISKE DSTERGENTSR FLUORALKYL OVERFLATEAXTIVS STOFFER Trade Secret Trade Secret RESTER AV ORGANISES FLUORKJEMIKALISR Trade Secret 36 - 40 = 35 20 1-5 1-5 Ikke kjent 3 VIKTIGSTE FAREMOMENTER. '-&1 M. o 10 0 7 f L9a9b3.1r2e-f2.no.: Page 22 o f 31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Viktigste skadevirkninger Heisefare: Kan irridere eynene og huden. Sikkerhet: Miljefare: Spesialavfall. 4 F0RSTSHJELPSTILTAK. Tiltak eyr.e: Skyll straks eynene med rikelige mengder vann. Kontakt lege umiddelbart. Tiltak hud: Skyll huden med rikelige mengder vann. Evis irritasjonen vedvarer/ kontakt lege. Tiltak innanding: Hvis symptomer oppstir, bring vedkommende bort fra ekspor.eringskilden. Serg for frisk luft. Hvis symptomene vedvarer, kontakt lege. Tiltak svelging: Kontakt lege umiddelbart. Fremkall brekninger kun i samraa med medisir.sk personell. Gi aldri noe gjennom munnen til en bevisstles person. 5 TILTAK VED BRANNSLUKNING. Egnet brannslukningsmiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: Forbrenningsprodukter ved brann: Prosedyrer ved slukking av brann: tiTif L99a3b.1r2e-f2.no.: Page 23 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM 6 TILTAK VED UTILSIKTET UTSLIPP. Personlige vernetiltak: Se vernetiltak under andre punkter i deute HMS-databladet. Tiltak ved uuilsiktet utslipp: Ventiler omridet. Spill avgrenses umiddelbart. Dekk til med et absorberende materiale. Spill sarnies opp umiddelbart. Rengjar omrdet med vann. Plasseres i en egnet beholder med lokk. Fjerning av kjemikalieavfall: Se punkt 13, Fjerning av kjemikalieavfall. - j j 7 7 KANDTSRING OG 0PP3EVARING. ! 3etingelser for sikker oppbevaring: Mi ikke lagres i omrider hvor produktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur under 49-grader celsius. Lagres ved temperaturer over 0 grader Celsius. Hold beholderen nett lukket r.ar produktet ikke er i bruk. Uforer.elige materialer: Ventilasjon: Oppbevar emballasjen p1 et godt ventilert sted. Sikkerhetstiltak for k forhindre brann: Sikkerhetstiltak for k forhindre eksplosjon: Ikkebrennbar-. Sikkerhetstiltak for forhindre statisk elektrisitet: Forholdsregler ved bruk: Hold emballasjen tett lukket. Rayking forbudt: Under bruk av produktet kan rayking forirsake forurensning av tobakken og/eller reyken og farlige dekomponeringsprodukter kan dannes . Se punkt 10, stabilitet og reaktivitet, skadelige dekomponenter. i 8 EKSPONERINGSKONTROLL OG PERSONLIG VERNSUTSTYR. 99312-2 Page 24 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Oyevern: Unngi k f4 damp, spray eller tike i oynene. Bruk tette vernebriller med ventiler. Hindvern: to 3ruk egnece vernehansker av hensiktsmessig materiale ved hindtering av produktet. Vernehansker av folgende materiale(r) anbefales: Butylgummi. Annet hudvern enn hlndvern: Unng4 hudkontakt. Andedrettsvern: Unngi innanding av luftbiret materiale. 3asert pi konsentrasjonen av luftforurensninger i arbeidsatmosfaeren velges en av folgende type(r) godkjent incedrettsvern: halvmaske med filter mot organiske damper og vaskeformige partikler: A/P2SL. Svelging: Det m4 ikke spises, drikkes eller roykes under arbeid med produktet. Vask utsatte omrider grundig med sape og vann. Vask hendene etter hindtering og for spising. Anbeialt ventilasjon: Kvis utsugningsventilasjon ikke er tilstrekkelig, mi- egnet godkjent indedrettsvern benyttes. Sorg for tilstrekkelig ventilasjon slik at campkonser.trasjonene holdes under anbefalte administrative r.ormer og/eller kontroller spray eller tike. Adm. normer for forurensnir.g i arbeidsatmosfare, Februar 1996 : VANN (7732-18-5) Anmerkninger: Ingen acm. norm. 2-(2-3UTOKSYETOK5Y)-ETANOL / (DIETYLENGLYK0LM0N03UTYLSTER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). 1,2-STANDIOL (107-21-1) 8-timers verdi ppm: - (stov) 8-timers verdi mg/m3: 10 (stov) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) SYNTETISKS DETERGENTER (Trade Secret) Anmerkninger: Ingen adm. norm. FLUORALKYL OVERFLATEAXTIVE STOFFER (Trade Secret) Anmerkninger: Ingen adm. norm. RESTER AV ORGANISKE FLUORKJSMIKALIER. (Trade Secret) 8-timers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. i9^9u3o1. r2e-j2.no.: Page 25 of 31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM 9 FYSISKE OG KJEMISXS EGENSKAPER. Tilstandsform, farge, lukt: Klar, ravfarvet vaske. pH : ca. 8.5 Kokepunkt/-intervail: ca. 100 C (Begynnende) Smeltepunkt/-intervall: Ikke relevant rlammepunkt: Ingen (Setaflash CC) Selvantennelighet: Ikke relevant Eksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, evre: Ikke relevant Damptrykk : ca. 14.3 mmHg (Kalkulert v/romtemperatur) Loselighet i vann: Blandbar. Spesifikk vekt: ca. 1.05 (vann*l) Damptetthet: ca. 1.21 (luft=l) (Kalkulert v/20C) Flyktige organiske forbindeiser (VOC): = 578 g/1 SCAQMD Kalkulert v/20C. Fordampr.ings hast ignet : < 1 (butylacetat=l) Viskositet: Ikke kjent 10 STA3ILITET OG RSAXTIVITET. Forhold som skal unngas: Materialer som skal unngis: Ikke kjent. Farlige spaltningsprodukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eller partikler. Dekomponering av bruksferdig blanding forventes ikke & representere noen risiko. 0 007 L9a9b3.1r2e-f2.no.: Page 26 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Stabiliteti og reaktivitet: Stabilt. Farlig polymerisesjon vii ikke forekomme. 11 OPPLYSNINGER OM HELSEFARE. Effekter eyne: Mild eyeirritasjon: symptomer kan vaere radhet, hevelse, smerter og t&rer. Effekter hud: Mild hudirritasjon {etter langvarig eller gjentatt kontakt): symptomer kan vare radhet, hevelse og klae. Effekter innndir.g: En enkelt overeksponering som overstiger anbefalte administrative normer, kan fere til: Pvirkning av sentralnervesystemet: symptomer kan vare hocepine, arhet, sevnighet, manglende koordinasjonsevne, r.ecsatt reaksjonsevne, slcret tale, svimmelhet og i ekstreme tilfeller bevisstleshet. Irritasjon (evre luftveier): symptomer kan vare sirhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsek utfcrt med organiske fluorkjemikalier, som finr.es i dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slavhet samt effekter som pvirker neurologi, bukspytt, binyre og hematologi. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Svelging kan- fere til: Pvirkning av nyrene: symptomer kan vare redusert urinmengde, blod i urinen og ryggsmerter. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer kommer ned i lungene. En alminnelig rsak til tilstanden er oppkast.): symptomer kan vare hosting, pustevansker, gisping, hoste odd blod og lungebetennelse som kan vare livstruende. Informasjon om allergi, overfalsomhet: Kreftfremkallende egenskaper: 000780 L99a3b1.r2e-f2.no.: Page 27 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Et resym5 vedrorende produktets giftighet er tilgjengelig pi forespersel. Dette produktet inneholder en eller flere organiske fluorkjemikalier, sora muiigens kan absorberes og lagres i kroppen over lengre tid, enten som utgangsmolekylet eller som nedbrytningsstoffer (metabolitter), og kan akkumuleres ved gjentatte eksponeringer. Det foreligger injen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Forekomsten av organiske fluorkjemikalier i blodet til den generelle befolknir.gen og underpopulasjoner som arbeidere, har vart publisert siden 1970 rene. 3Ms epidemiologiske studier av egr.e arbeidere viser ingen skadeiige effekter. : k i 12 OPPLYSNINGER OM MILJOFARE. Persistens/Biologisk necbrytbarhet: Dette produktet inr.eholder en eller flere organiske fluorkjemikalier som muiigens ikke er nedbrytbare og forblir i miljaet (persistente). Andre skadevirkninger, milja: Nedbrytbarhet: COC {Chemical Oxygen Demand) = 1 , 1 g/g; 30D 20 dagers (Biochemical Oxygen Demand) * 1,1 g/g; BOD 20 dagers/COD = 1,00. Akvatisk giftighet: Statisk 96 timers LC50 for fisk (Fathead minnow (Pimephales promelas)) = 750 mg/1; Statisk 96 timers LC50 for fisk (Rainbow trout (Oncorhyncus mykiss)) = 1300 mg/1; Statisk 48 timers LC50 for daphnia (Water flea (Daphnia magna)) 1600 mg/1 (1300-2100 mg/1); Statisk 48 timers LC50, Amfipode (Scud (Gammarus fasciatus)) = 1100 mg/1 (840-1300 mg/1); 48 timers EC50, Eastern Oyster embro-larvae (Crassostrea virginica)' - 47 mg/1 (10-234 mg'/l); 48 timers LC50, Common mummichog (Fundulus heteroclitus) * 2500 mg/1 (1700-3600 mg/1); 00 3781 L9a9b3.1r2e-f.2no.: Page 28 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM (360-710 mg/1) ; IC50 Respirasjon, Activated sludge ** > 1000 mg/1;^ TTC (2,5,5-trifenyltetrazoliumklorid) test viste ingen ensymhemning for konsentrasjoner opp til 1000 mg/1. Sarg for avfallshindtering iht. gjelde.nde forskrifter. 13 FJSRNING AV KJEMIKALISAVFALL. Metoder for fjerning av kjemikalieavfall: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mottaks- eller behandlingsanlegg for spesialavfall. Anbeialte avfallsgrupper: HOVSDKATEGORI 07: AVFALL FRA ORGANISKE PROSESSER. UNDSRXATEGORI 07 06: Avfall fra P3D3 av fettstoffer, sipe, rengjaringsmidler, desinfeksjonsmidler og kosmetikk. EAK-kode 07 06 03: Halogenerte organiske lasemidier, vaskevasker og moderlut. Avfalistoffr.ummer: 7151 Organisk avfall med halogen. Spesielle komponenter: inneholder flourforbinde1ser. 14 OPPLYSNINGSR OM TRANSPORT. ADR-klasse: siffer: bokstav: margnummer: CEFIC-kort: UN-nummer : ICAO-klasse: IMDG-klasse: IMDG-side: EMS-nr.: MFAG: L99a3b1.r2e-f2.no.: Page 29 o f 31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING F O A M Ovrige opplysninger: Ikke transportfarlig gods. 15 OPPLYSNINGER OM LOVER OG FORSKRIFTER. Merkeetikett: Symbol(er): HSLSESXADELIG (X n ) Inneholder: 2-(2-butoksyetoksy)-etanol 35% Etylenglykol 20% R-setninger: Farlig ved svelging. Kan irritere eynene og huden. S-setninger: Unnga inn&nding ev damp/spreytetke. Unngi kontakt med huden og eynene. Far man Stoffet i eynene; skyll straks grundig med stre mengder vann og kontakt lege. Etter hudkontakt, vask-med sape og vann. Ved svelging, kontakt lege omgaende og vis denne beholderen eller etiketten. Kun til industriell bruk. Ikke beregnet til bruk som medisinsk utstyr eller legemiddel. YL-gruppe: 00 Korr, yrkeshyg. luftbehov: Ovrige opplysninger om merkeetiketten: Ovennevnte merking er basert pa tidligere merkeforskrifter og vil vare k finne p produktet en tid fremover. Merkingen vil imidlertid erstattes av felgende merking, som er basert p reviderte merkeforskrifter av januar 1993. Symbol: Ingen Inneholder: 2-(2-3utoksyetoksy)-etanol, 1,2-etandiol Kan irritere eynene og huden. UnngS. inninding av damp/spreytetike. Unnga kontakt med huden og eynene. Fr man stoffet i eynene; skyll straks grundig med stre mengder vann og kontakt lege. Fir man Stoff p huden, vaskes straks med stre mengder sipe og vann. Ved svelging, drikk to glass vann og kontakt lege umiddelbart. YL-gruppe: 00 000783 L9a9b3.1r2e-f2.no.: Page 30 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Spesielle lover og bestemmelser: 1. Forskrift om utarbeidelse og distribusjon av Kelse-, Miljo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift om klassifisering og merking av farlige kjemikalier. 3. Forskrift om klassifisering, merking m.v. av stoff som kan mediate miljafare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfall. 6. Etc. 16 ANDRE OPPLYSNINGER AV 3ETYDNING FOR BRUXZRNSS SIKKZRHST OG HELSZ. Anvendelsesomrder: Filmdannende skumkonsentrat for slukking av brann i hydrokarboner. Viktigste kilde(r) ved utarbeidelsen av HMS-databladet: Datablad fra 3M General Offices, St. Paul, Minnesota, USA. Arsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og KMS-databladforskrifter, fastsatt i januar 1998. Etc-. Egne opplysnir.ger: Informasjonen p dette HMS-databladet er i henhold til vre nvrende oppiysninger og beste oppfatning angende riktig bruk og hndteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medforer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. 00'0784 L99a3b1.r2e-f2.no.: Page 31 o f 31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM FOLGENDE PRODUKTES DEKKES AV DETTE HMS-DATA3LADET : 3M ID-nr. : XF-0008-0217-3 3M AFFF FC-203 Brannslukningss kum Produksjon I 3M ID-nr. I : XF-0008-0099-5 3M Light Water 3% AFFF Fire Fighting Foam, FC-203,1000 L 3M ID-nr. : XF-0008-0070-6 3M Light Water 3 AFFF Fire Fighting Foam, FC-203, 200 L_ 3M ID-nr. IiI ! I : XF-0008-0069-8 3M Light Water 3% AFFF Fire Fighting Foam, FC-203, 20 L I I I I 0001785 i