Document G6E5mgo4vw5jJ2MVO2G2a5mEY
AR 226 -- 0842
MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE
Identity: ArefmeirxrteudretocaosntPaiFnOiSng, pFeCr-f9i5u,oroorocatsanaescuolmfponoanteen,twohficFhC-m60a2y. al(s1o- be Octanesulfonic acid) (CAS # 2795-39-3).
6R0e2m.arCkusr:rent
Tihnfeor3mMatipornodiuncdtiicoantelsotitniusmabemrixwtuarse
501. The test of 1% PFOS,
sample 12%
is
FC-
dfioeatmheyrl,en1e%glsyocdoliubmutoycltyelthseuri,fa6t1e,.015%% swaotdeiru,m2d0ec%yletshuyllfaetnee, g1ly%coml,ag2n%esSiulutmone
sulfate, 0.7% chloride, and
0x.an0t5h%antolgyutrmi,az0ol.e.1%
starch,
0.1%
n-(3-chloroallyi)hexaminium
cThhaerafcotlleorwiiznegd csounmcmenatrryataipopnlsioefsitmopuarmiitxiteusr.e Dwaittha imnacyomnpolteatcecluyrately reflect the toxicityofthe fluorochemical componentofthetestsample.
METHOD:
Mthod: Microbics Microtox *BASIC Procedure
GTeLsPt:tyNpoe: Acute static
YSpeeacriCeosm:plPehotteodb:ac1t9er8i8um phosphoreum
Analytical Exposure
monitoring: pH and period: 15-minutes
osmotic
condition.
Sptraotgirstaimcaplromveitdheoddfsor: MiEcCr5o0tovxalues calculated by statistical linearregression TTeesstt coorngdaintiisonmss:ource: Microbics' Reagent.
Diluent: Microbic's 2% NaCl Reagent
Test temperature: Not noted.
Sprteopcakraedndbytedsitsssoollvuitnigo2n0p0rmepgaorfatteisotn:suAbsptrainmcaeryinst1o0c0k msoLloutfiMoinlwiaposre
Mill-QTM 6.7. An
owsatmeort.icTahdejupsHtomfetnthewasstomckadsoeluutsiionngw2a0s0
5.5 mg
and adjusted to NaCl dissolved
in
10 mL stock solution. Appearanceof test solutions was noted as
"teasptpasroelunttiloyncsowmeprleetmelaydmeisbcyibplreopionrtDiIownaatledri,luntiooncso.lor apparent". Al
ENxupmobseurreofvreespsleilcsa:te4s:mtLwcouvettes.
----
ENluemmbeenrtobfasciosn:cePnetrrcaetnitonlisg:ht Floosusr plus a blank control
002110
RESULTS
NEloemmiennatlvcalounec:entrat5i-omnisn:utBelaEnCksoco=nt2ro1l0,
125, (190
250, 500, and -- 230) mg/L
1000
mg/L.
15-minute ECan = 220 (2--02300) mg/L
Element values based on nominal concentrations.
Remarks: Substance
TReesmtianrgkwsafiselcd.onTduhcetveadlouensarempioxrttuerde
as described apply to that
in the Test mixture and
not
the fiuorochemmical proportion alone.
CONCLUSIONS
dTehteerFmCi-n6e0d2to15b-emi2n2u0temgE/CLsowfiotrh Pah9ot5o%baccotnefriiduemnpcheoisntpehrvoarleoufm2w0a0sto 230 moll.
Submitter: 3M Company, Paul, MN, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
DATA QUALITY
HReolwieavbielri,tys:amKpilimeispucrhitryanwkaisngn2o.t pTreospteirnlgymceheatrasctalelrcirzietderiaanfdorthqeuasltituydytelsaticnkgs. analytical confirmation of the amount of fluorochemical proportion in the
solution.
REFERENCES
This study was Minnesota, Lab
cRoenqduuecsttednubmybtehre
3M Environmental F1151, 1988.
Laboratory,
St.
Paul,
OTHER
Last changed: 6/26/00.
002111
ENVIRONMENTAL LABORATORY MICROBICS MIcROTOXR SvsTEM
RAW DATA
Ww ELIS] Paty
TTEeSsTt DsATuE:se: -E2C8- 02 Liat tiatecATCPlus(41-aloo-goil-p1 50
ANALYST:
swe cuverre |rss operon|EE
Q
Sn. ALTsREApDIES s0-nmn.
:I(5)
1(15;
1(30;
81
BLANK CONTROL
ao 76 | 73
a
DUPLICATE
feo 183 | go
82
125
BA
43
Ha
2
Duplicate
lox| 50 49
8
250
ar | Ho | 34
Duplicate.
loa | 37 39
8c4o DSuoploicate a9l9 E2X7] 2226
%
1000
ad |e 15
cs
Doplicate
a8 le /s
Test Substance 9 Viscous bron Ligand
StApopcakresnotllyulCeonm,ple2t0e0lymymisic1i6b0 lme.2 IOnT.dTwater. Fach, /nd= 2m.
Stock
Osmotic.
soln. PHS.
adjustment -
dpsed
ay, acum
6:w7adewri,sh,nodielsultoer.apMpaoahr.
Mal to lon. Stock soln|
Diloent
Microbe, go, Vall Solusion
Tibial Dilutions = TTehnittoinaeld Caqbnoievnet.ratTiounestnwere.is4 rdhouoeblme;.e Ththee.nIanilrodaustsead) m[ c ie
OF Waters Distal Ra1d-24-25E+eeAtaiamnsdSetlrleotrhseBn ySransHteoregd_.S(52/50)OOinPtIotAhSe.
.
JANE patiosrHn inal
MICROBICS MICROTOX SysTay
MICROTOX DATA REDUCTION
FC-toa Light Water Are.
SANK cuvETTE
81
[E 7v6afniA eo 00S .0yif]or0e.rm0e)nce[[fBavwrg-eosssitmi]rooeeor n
The blank ratios for Bl and CI should agree within ] 0.02, on
a
ate_I-23-29
Analyst. =
Plog (H1-36c0- 9011-0 1,
[ame rT]
-- p---- --m1
{EAN BLANK RATIOS ----MBl BM K RAu TIO +NCl o BKmRAs mTIO o
1220 GH 1055 (Carmen) tate Bank tatsx usil seme tention,
Ee
est Concentration
-- EF
2.5490
5.5T7e4 wTr
52;2/32550
i
male 0.707
[heey
o.tg
ieee
i500 3 /eco
[2237g% [503
Tae Tysae
LIcHT Loss (1a) = --1FF --x 100
re 1
Test Concarntratiam
PWN.fa1
37
Tem
taTera
33a&2 s450
ma"S JL
41
|5
3Y6o
es
4 sco [| s7
Yioco [el Te
SE Ce ws som,[me Te | EFFECTIVE CONCENTRATIONS (EC"s) Ke)
Calculated using "[Y,C* data pairs.)
C5 2 (IST 2/Contr6a CHE230)
TM 9ic
[amqee(0gegasyT ] T] 22 PQ XL Yor SIE FIe5F 352020"