Document G68qD8eyX9qgD3Nm9mmBv370n

AR 226 -- 0756 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: _ The 3M production lot numberwas not noted. The test sample is FC-203CE. Current information indicates itis a mixtureof 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyitriazole. The following summary applies to a mixture With incompletely characterized concentrationsof impurities. Data may not accurately reflect the toxicity of the fluorochemical componentofthe test sample. METHOD: Method: OECD Method 202 Type: Acute static GLP: No Year completed: 1991 Species: Daphnia magna Supplier: In-house cultures. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: -48-hours Test organism age: <24 hours old Statistical methods: No calculations performed, based on study results. Test conditions: Dilution water: Filtered natural well water Dilution water chemistry: Conductivity: 840 ymhos/icm pH: 76 Lighting: Cool-white fluorescent lights at 20 uEin/sec/'. A daily photoperiod of 16 hours light and 8 hoursdarkwas maintained throughout the testing period. wSatoscpkraepnadretdesitnsdoilluuttiioonnwaptreerp.arAaltiiqouno:tsAw1e0r0e0amdgd/eLd pdririemcatrlyy tsotodciklustoiolnutwioanter -- in test vessels to create test solutions. Exposure vessels: 250 mL beakers containing 200mL exposure solution at a water depthof approximately 6 cm. Number of replicates: 4 Number of daphnids per replicate: 5 001143 Numberof concentrations: five plus a blank control Water chemistry during the study: pH range (048 hours): 77..66 ~- 88..33 ((c1o0n0t0romlge/xLpoesxuproes)ure) Temperature range (048 hours): 1199..00 --2200..21 CC ((c1o0n0t0romlge/xLpoesxuproes)ure) Conductivity range (048 hours): 887400 --- 888700 ppmmhhooss//ccmm ((c1o0n0tr0omlge/xLpoesxuproes)ure) Dissolved oxygen range (048 hours): 8.59.2 mgiL (control exposure) 8.59.3 mg/L (1000 mg/L exposure) RESULTS - Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L. Element values: 48-hour ECs= >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described inthe Test Substance Remarks field. The values reported apply to that mixture and not the fiuorochemical proportion alone. CONCLUSIONS `Thetestsubstance 48-hour ECs was determined to be >1000 mg/L, the highest concentration tested. No mortality or sublethal effects were observed at this concentration. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking = 2. This study meets the criteria for quality taensdtiingt.lacHkoswaenvaelryt,ictahlecsotnufdiyrmlaatcikosnoinfftohremaatimoonuonntopfurfitlyuoorfotchheemtiecsatlspurbosptoarntcieon in the solution. REFERENCES This test was conducted by EnviroSystems Division, Resource Analysts, Incorporated, of Hampton, NH at the requestofthe 3M Company, Lab Request number J1884-1, 1991 001144 +L Last changed: 6/26/00. 001145 RAE ID TSR Foe mt ee a WAILABLE B a yt BESTCOPY A hr StodyTitle. #1 * static Acute Toxicity of Fc-203cE f--sb--o bi LTE kai Banas he AREER al Sd pe 5 Hue, Ten EC SER CoaaV Slag E be ab a Ci omo he a fi Le So Eo b : Sm Ee } S5 aad B SEReh Shik BhR oauE iniann EA sk ee esva hd rd io Apna. To 5S GREAT : : : st : BESTGOPY AVAILABLE . i" =3 SCionimvptasdTninhyece"neibfse`oaGdrFt.eR8"edsatoouhnroihcueiertsts'yAdnkutiriorifoenncg)sY,JCurIseTspOaoXcr3Et0.S-tnotoBu1"2pT,tthheo1en9t,9e1ds,tubpohvnteatisMdhe,cnepoemDsFtuioipeeehehraseeiodstymHSaeiatnovrsnes,ts Scoonrdeulctetdsa PbyaotShuysaerd:Magar, Teter Rowilat, Bien Stanford, Abert TctoientmcepurTenhrtaeetcauotrlieltoeenctstotedofw"3a0st.t1eIsHvtpucep.cttsooun"rb,TmshteeadfadocrieluwBtue4xip3ors8nbivrasa.ett.eartSiicRriaeasrtaictoFoiniioltnvtioevtroaeensrdssscooorwntitotesrnhae]pllosLfyeiiel'vdsle eINo0rOaeiR"miaSalllEuttCaoltonncaadnitsrsaaotTllivotaendst$a0tr0`oyFoe3e0nnT1C,cIoOn4Sc3Ee6.nt1r,aw0te0ti0eornasf0lse.hsolrheonbtinesoeendseeecopoltnsceeln1et5m0netSciyhostn,.s B1G2ol8uorisR t"ewad5r0e acerrnmasneosft.ua0'.t0h02e001c`ob3no.turtoscy le0s1siddtaiapnthgaitdhisen ashaatdcokndioifeastShtaevteerraeson-- eteebvertoM0-- eseioighh3:t0 3ET1rr1po"osrtuedrrueshtahia"odnfs1d1uw0se0kr0aeiwdaa'l`i.a tFogeo-otBfh0e3Htc.eosntdistuisosntan`ictetFheeseuigiieadsiinneaof48thheouratBeesk.o a fi 44 R S FaigtE et ; : Un poppe Bre 0 gS i ER eor HS aEneae 5s 0 akefrat y T SE E Bre i a GG &SArbaa slim nC s OT e oL na phB ac Snh. a ge ge RARE LET Bi r-ie 4 foie e i} n i Eu RRER a EE Veaeaa ee n i peicachobEE e a iat] RL aks fon - ' J 004147 RE ER : ig ET Bee COPY AVAILABLE 7: 1. Tisux or comms . ; p-- nar : y? I. Sumaary 2 ZA I. Introtuction s % Vo ettots and materials 5 % Ln mest s Brood ERR SRE Cosy MG or ERE ER is 4 Ea ge Eh lee i N EAREA E eR a SRR { t JR nI e 11 ABEL ) pSAd RITA. A i gB a ea. e THEATERHe Pa ' : ig PR EEE ae y 3 oe oeire BBEESTICOPY: AVAILABLE i AE Td : } 1. oe or mavtereesss wi TOIL andes curictartition of sopromtative nar TSASR sample of wa natural well water used as .dilution B SL TRIE seri daa fro toeity tent 5 fis Toear3. tetnetntre tensetion fen M Tie ho.ScTomctocttay,coan, ocomTpeosrtes,osroetslsLiolveettly 13 | eg Seve wi Si [SilBSe os ay% eS S Hive Siks ag i d atataA a SeR l e sEloA aCRY L 0 a Se USIl Ee " | Se ERR EIR RE 8 ] S choie C iy 0 e ee LR Sho Ne a ra wer on { Bi LoLE Ea eeO, a 0 ii ol oe ae | Sp ER Ta ] 3 Gh na ie |Ent, 5 - rd QiFi Rne TH BESTECOPn YZAi VAILaABLEBe : b IV. INTRODUCTION aiSJTEbnhrstehmesterhrTltehiitsnGroeeru"ssatT1sutadhtySoiAaeewEnsaostyscAao"UsmTpIatobInvesrvOoeedTprioeTordbtItsTbusyLsiTIIooelNtLtocChre CooeemnRdpntEaacnJIiyoeL,n JhoSe etn.TSSPvaoauenlk,iA neiWesdnneCsrtothrsse.sa,eAnTethmsee N v. rns am rmans rest smn; - - 3i ~~ EF BBBorereiieonns["rJsCsa-n2c T1p0soJCeaoEslolsde(sMEaHLuyeiviima3rtIrno5haeSteyaIsstitP.eoeLm...srciA3Soo8abtmTiepooaltttseooegaesWpeatmasoabtlmeorprnnlseee.v2te9eoit35opKMne)oeasewainistepedeheeeli1veSniererearandlael]tTteo Vii 3 kechived ae ovbrotatens tor motuns ot 15 penn: [i p-- wd3" 32 eEeusrttaieebnidgrTtea.trneetussiwaTt""eeeSdsemlCveEiafcsotcrshstaost"rceEecnrdltoadsotviaSeol0in-lvgoeftaitS1oenstB0poSoirleurrnrpiberoeinresnam,atdteioeftobsxboaSe1aeenertenantaictheieteTny } 65 E30g prTEoSe T ioRngtamasn:reoy elsyetbialooe ae spEedrsepSrrsaeiass wsennse; fBniatTle 6GB HideB l m RI ea hue ricE oni ctunitnS on n o"nofl dee ltllheIseRtaorxirceiem try muisttasete dBAePsHoSremtilosteetsstainngdCg,yar4sHdupTLheIiaietevrerteoen 5 EB ( Le S e L r l e R sAeS o nse S nR RSeRReB a nnista en trL eaasd hyset, : 0 B i E n E e a ee e i r EE e a SSe e i alfripstsaochEE RLR al ET es Cao ES 4 fs: om Ae ie . .% : RELCE Anh he ' N ps; LA 001150 W2e ow . : BESTCORY AVAILABLE ---- torrTT WR " Conductivity ushos/cn = uo 2 "orga: Storie wart es w wp wn ot o SA2e30 R {8Roa t1o.7rW:ome dceatetcattteoydFamboive;tsepereTiheToydeRveIe E. iR E E l 1 CtccloeacutaestiGonoogf orernienuBsaaetdsaonntiseitt:naundt3yc dtaetianfgs. de Ee SECoolrmlal = Boies WiSlEER ROOBEECa oRbEBv iA oTC y k rE CEE 9S3 a Le E ode ol na fem i fiiRa Ra EINnSon | fs 1 ae BP e Le hd ' imine ind : CT Nim on 1 ET - } Tomer masTov: : E1o9a9t1i.ctaTdh"ePhesdtaTtEsintThtuaiarvnaedttoSonxmipcairrttaycteotirenestteorvfaso3f0peS5chte1o0rcahs4ed1tdh(u1cFi0inv0ge.dcuonnecen10vtorsattotiob1na3,sb g SCSoontEtaetaiUtornatihtoaanscskaT`ocoFremlithntee"ad.HtesvEittMhaeunite.eScisathlheremeesseceseom0roafakosl3 (sCoAolnv0veen0etl,,e+1e5lH0owssiehroeegr:tk w-- S50 SL, A00 n 400 ng, 0d 1,000 37h cBeorpbleircsuTtveestshtaytBE cdo"aenatscahhinaetdreors3et0a0Fe,a4sd1onTlh5ya seogsdttesquaasslolhByurbiidooirnseterdiebauIttneerd25a0dm-ao0np1gthghfavousares ; TSTopePrnuoeoctaitaonr"tthealtyurTionEcgkionn`T.he of48Tebunothuevevvaeesrstesle)l(:s1 rwAuanbc1oo6mbboreuantnbdeoSrinsg"lhsttabs4lnredeann3hosehtowueseIddnarobknp . 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