Document G67RKZqo85jLzgEm41Y659RV4

..N* t ;V , XV r The Tftxlolty of the Vapor o t Arooiora apirllfolt . vv toy .i-. Joseph P, Treon, Ph,D,. Prank P. Cleveland, 11,D. JohnOappel, B,8, Prederlb X. Shaffer, M.S. Balph W, Atohley, B.S, J o h n ? , Torhe ok, B,S, 'r - V' j ' V , ' : "K tx v /' Vv ' ' ''v, i J. ' i. ' June fii, "1955 -f ' ' THE KETTERING LABORATORY .v. ''l\Vh / / },..> v . ~ *n thf v. ( " . ''\;' ' . [ ' poportmont of Pfoytnkiva Modictpo and Industrial Haalth ';(''N j ; Colltga of Madicint , UNIVERSITY OF; CINCINNATI, CINCINNATI, OHIO % "-) 1- 1 a' l. r . - , hons oaaao9 . ./! v .r: Saguenoe of Tables 1 . The Mortality, Length of Survival, and Changes In Weight of Animals Exposed to the Vapor of Aroolor 1242 In Air. Experiment No. 1. 2. The Mortality, Length of Survival, end Changes In Weight of Animals Exposed to the Vapor of Aroolor 1242 In Air. Experiment No. 2. 3. The Mortality, Length of Survival, and Changes In Weight of Animals Exposed to the Vapor of Aroolor 1234 In Air. Experiment No. 1. 4. The Mortality, Length of Survival, and Changes in Weight of Animals Exposed to Conditioned Air Containing No Vapor of Aroolor 1242 or Aroolor 1234. Experiment No. 1. 3. Summary of the Data on the Mortality Among Croups of Ex perimental and Control Animals. 6. The Average Changes In Weight of Experimental and Control Animals. 7. The Relationships of the Weights of Certain Organs to the Body Weights of Animals Exposed to the Vapor of Aroolor 1242 or Aroolor 1234. t . Summary of the Data on the Relationship of the Weight of the Liver or tha Kidneys to the Body Weight of Animals Exposed to the Vapor of Aroolor 1242 or Aroolor 1234. 9. The "Prothrombin Time" (Method of Kato) of the Blood of Animals Eollovring Their Exposure to the Vapor of Aroolor 1242 or Aroolor 1234 In Air. 10. The "Prothrombin Time" (Method of Kato) of the Blood of Animals Pollowing Their Confinement In Cages Supplied with Conditioned Air Containing No Vapor of Aroolor 1242 or Aroolor 1234. 11. The Effeot of the Exposure of Cats end R A b l t s to the Vapor of Aroolor 1242 or Aroolor 1234 in Air for 7 Hours per Day on 3 Days per Week: Over a Period of Several Months, Upon tha Apparent Prothrombin Oontent (Method cf Kato) of Their Blood* mons oaaaio 11 12, The Effeota of the Intermittent Inhalation of the Vapor of Aroolor 12*15 or Aroolor 125**, or of Conditioned Air, upon the Numbers of Erythrooytea and Iieuoooytes and upon the Conoentration of Hemoglobin In the Peripheral Blood of Animals. 13, The Average Numbers of Erythrooytea and Leuoooytes and the Average Conoentration of Hemoglobin in the Peripheral Blood of Experimental and Control Animals. 14, Application of the "t" Teat to the Hematologioal Data Ob tained in Observations on Exposed and Oontrol Animals. SONS 088811 Ill Sequence of Figures A. Equipment for Volatilizing Aroolor, Chamber and Equipment for Combustion and Collection of Samples. B. Front View of 600-Liter Chamber. C. Humidifier for Furnaoe Sampling Towers. D. Sampling Tovers. X. Standard Curve for the Determination of Aroolor 1242 and Aroolor 1254-. 1. Weight of Cat A-446 Recorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 In Air 3.6 Miorograms per liter (Experiment No. 1), 2. Weights of Individual Guinea Pigs B -331 to B -336 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 3,6 Miorograms per liter (Experi ment No, 1). 3. Average and Range of Weight of Guinea Pigs B -331 to B -336 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 121(2 in Air - 3.6 Miorograms per liter (Experiment No. 1). 4. Average and Range of Weight of Mioe B -251 to B-260 Re oorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 3.6 Miorograms per liter (Experiment No. 1). 5 Weights of Individual Rats 1-436 to 1-440 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 3.6 Miorograms per liter (Experi ment No. 1). 6, Weights of Individual Rats 1-441 to 1-445 Reoorded at Semi-weekly Intervals During Esp oaure to the Vapor of Aroolor 1242 in Air - 3.6 Miorograms per liter (Experi ment No. 1). m u n s uaaaiz f 7, Average and Range of Welg&t of Rata L -436 to L-445 Re- oorded at Semi-weekly Intervale During Exposure to the Vapor of Aroolor 1242 in Air - 8.6 Mlorograss per Liter (Experiment No, 1). ? 8. Weights of Individual Rabbits 1-374 to 1-377 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 124-2 in Air - 6.6 Miorograms per Liter (Ex- 1 perlment Mo 1 ) 9 Average and Range of Weight of Rabbita 1-374 to 1-377 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6 . 6 Miorograns per Liter (Experiment Mo. 1). 10. Vieight of Oat A-445 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6.8 Miorograms per Liter (Experiment No. 2). 11. Weights of Individual Guinea Pigs B-325 to B -328 Re oorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6 .6 Miorograms per Liter (Experiment Mo. 2). 12. Weights of Individual Guinea Pigs B-329, B-330 and B-419 Reoorded at Semi-weekly Intervals During Exposure to the Vapor or Aroolor 1242 in Air - 6 .6 Miorograms per Liter (Experiment Mo. 2). 13. Average and Range of Vieight of Guinea Figs B -325 to B-330 and B-419 Reoorded at Semi-weekly Intervals Dur ing Exposure to the Vapor of Aroolor 1242 in Air - 6.8 Miorograms per Liter (Experiment Mo. Z ) . 14. Average and Range of Weight of Mioe B-241 to B-250, B-376 and B-464 Reoorded at Seml-v/eekly Intervals Dur ing Exposure to the Vapor of Aroolor 1242 In Air - 6.8 Miorograms per Liter (Experiment Mo. 2). ,15. [ Weights of Individual Rats L-526 to L -530 Reoorded at Semi-weekly intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6.8 Miorograms per Liter (Experi ment Mo. 2) . ! MNS 0 6 8 0 1 3  16. Weights of Individual Rats L-531 to L-535 and L-d36 Re corded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6 , 6 Mlorograms per Liter (Experiment No. 2). 17, Average and Range of Weight or Rats L-526 to L-535 and J.-656 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6 , 6 Mlorograms per Liter (Experiment No. 2) l6. Weights of Individual Rabbits I-370 to 1-373 and I-964 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6,6 Mlorograms per Liter (Experiment No. 2) 1?, Average and Range of Weight of Rabbits 1-370 to 1-373 and 1-964 Reoorded at Semi-weekly Intervals During Ex posure to the Vapor of Aroolor 1242 in Air - 6,6 Micro- grams per Liter (Experiment No. 2). 0. Weight of Oat A-444- Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254 in Air 5.4 Mlorograms per Liter (Experiment No. 1). 21. Weights of Individual Guinea Pigs B-319 to B-322 Re corded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254 in Air - 5.4 Mlorograms per Liter (Experiment No. 1). 22. Weights of Individual Guinea Pigs B-323, B-324, B-415 and B-4l6 Reoorded at Semi-weekly Intervals During Ex posure to the Vapor of Aroolor 1254 in Air - 5*4 Miorograms per Liter (Experiment No. 1). 23. Average and Range of Weight of Guinea Pigs B -319 to B-324, B-415 and B-416 Reoorded at Semi-weekly Inter vals During Exposure to the Vapor of Aroolor 1254 in Air 5.4 Mlorograms per Liter (Experiment No. 1). 24. Average and Range of Weight of Mioe B -231 to B-240 Reoorded at Semi-weekly Intervals During Exposure to the Vanor of Aroolor 1254 In Air - 5. 4 'Mlorograms per . Liter (Experiment No. 1). mums oaaaw vi 251 Weights of Individual Rats L-299 and L-517 to L -520 Re corded at Semi-weeklv Intervals During Exposure to the Vapor of Aroolor 1254 in Air - 5,1+ Miorograms per Liter (Experiment No# 1), 26. Weights of Individual Rats L-521 to L -525 Recorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254- in Air - 5*+ Miorograms per Liter (Experi ment No, 1). 27, Average and Range of Weight of Rats L-299 and L-517 to L-525 Recorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 125*+ in Air - 5 .1+ Miorograms per Liter (Experiment No. 1). 2, Weights of Individual Rabbits I-362 to I-36I+ Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 125*+ in Air - 5 Miorograms per Liter (Experi ment No, 1). 29. Weights of Individual Rabbits 1-1+55, I-57, 1-71 and I-87 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1251+ in Air - 5 A Miorograms per Liter (Experiment No. 1), 30. Average and Range of Weight of Rabbits I-362 to 1-3*+, 1-1+53, I-57, 1-71 end I-887 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 125*+ in Air - 3,>+Miorograms per Liter (Experiment No. 1). 31. Weight of Control Oat A-l+35 Reoorded at Semi-weekly Intervals During the Period of Confinement in Oonditioned Air. 32. Weights of Individual Control Quinea Figs B -313 to B-316 Reoorded at Semi-weekly Intervals During the Period of Confinement in Oonditioned Air. 33 Weights of Individual Control Quinea Pigs B-317. B-318, B-I+o e and B-576 Reoorded at Semi-weekly Intervals During the Period of Confinement in Conditioned Air, 3*+. Average and Renge of Weight of Control Quinea Pigs B-313 to B-318, B-l+02 and B-57o Reoorded at Semi-weekly In tervals During the Period of Confinement in Conditioned Air. HONS 0 8 8 8 + 5 Til 35, Average and Bangs of Weight of Control Mine B-221 to B-230, B-499, B-531* B -532 and B -533 Recorded at Semi- weekly Intervals During the Period of Confinement in Conditioned Air. 36, Weights of Individual Control Bats L-507 to L-512 Re- oorded at Semi-weekly Intervals During the Periods of Confinement in Conditioned Air. 37, Weights of Individual Control Rats L-513 to L-516 and D - 7 W Heoorded at Semi-weekly Intervals During the Period of Confinement in Conditioned Air. 36. Average and Bangs of Weight of Control Bata L -507 to L-5I0 and L - 7 W Recorded at Semi-weekly Intervals During the Period of Confinement in Conditioned Air. 39 Weights of Individual Control Rabbits I-365 and I-366 Heoorded at Semi-weekly Intervals During tbs Period of Confinement in Conditioned Air. 40. Weights of Individual Control Rabbits 1-363, I -369 and 1-975 Reoorded at Semi-weekly Intervals During the Period of Confinement in Conditioned Air. 41. Average and Range of Weight of Control Rabbits I -365 to I-369 and 1-975 During the Period of Confinement in Conditioned Air. HONS 088816 The Toxlolty of the Vapor of Aroolors 1 2 ^ ana lg5k Soope The purpose of this investigation was to determine the effeots upon animals of various speoies, of moderately pro longed Intermittent exposure to air bearing the vapor of either Aroolor 1&4 or Aroolor 12511 This report deals with the re sults obtained in experiments in whioh, for periods ranging up to It months, animals were subjeoted to respiratory exposure to air oontaining the Aroolors in oonoentrations that approaohed saturation and so were greater than any likely to be encoun tered in industry. The animals failed to exhibit any signs of lntoxioation during or after the exposure to which they were subjeoted, and the numbers of deaths whioh occurred among the exposed animals were comparable to those whioh resulted from incidental causes in the oontrol groups. Gross and miorosoopio examination of the tissues of the animals of both the exposed and the oontrol groups disclosed the axistanoe of oertaln abnormalities whioh did not differ signifloantly in their severity or frequency of ooourrenoe among the test and oontrol groups. Other experiments whioh Involve more pro longed exposure on the part of animals to lower oonoentrations of theso materials in air, are now in progress. MONS 0 8 8 8 1 7 -2- Summary of Results 1 . No signs of intoxication were observed in any of the numbers of a group of animals, all of which survived through out a period of 24 days, on eaoh of XJ of vfoioh they were sub jected to the inhalation of air bearing d .6 miorograms of Aroolor 1242 per liter (0.43 ppm) t o r 7 hours. The vlsoera of representative animals that were examined miorosooploally were normal. 2. The incidenoe of mortality among the animals of all speoies, excepting the rabbits, in 2 groups that were sub jected (7 hours on eaoh of Z or 3 days over a period of 120 or 121 days) to the inhalation of air bearing, respectively, Aroolor 1242 in the oonoentration of 6.S3 miorograms per liter (0,66 ppm), and Aroolor 1254 in that of 5,40 miorograms per liter (0.4l ppm), v/as no greater than that among a similarly constituted oontrol group. Two of 5 rabbits died following exposure to the vapor of Aroolor 1242, as did 3 of 7 rabbits exposed to the vapor of Aroolor 1254, whereas only 1 of 5 of the oontrol rabbits died. The survivors, with the exoeption of the guinea pigs exposed to the vapor of 1254, grew equally as well ae did the oontrols. The average weights of the livers and kidneys (relative to body weights) of the animals eg>osed to Aroolor 1242, did not differ signifleantly from those of the muns oaaaia -3- unexposed group, but the livers of the rats that were exposed to Aroolor 1254 were enlarged. The prothrombin oontent of the blood of the animals exposed to either Aroolor, as measured by the method of Kato, remained within normal limits. The deaths that ooourred among the test and oontrol ani mals were the apparent result of an appreoiable incidence of pneumonia. The lesions of certain of the animals were those of a frank pneumonia; in others such lesions were not fully de veloped. Degenerative changes in the visoera were usually found, in varying degrees of severity, in association with the pneumonia, but in oertain animals, test and oontrol alike, the degenerative ohanges were more evident than the pneumonia. The similarity of the lesions in test and oontrol animals, and the laok of oharaoteristio evidence of ohemioal pneumonitis, lead to the reasonable,but not altogether oertain conclusion that all of these fatalities resulted from interourrent disease among the animals, and not from the effeots of their exposure to the Aroclors. For practical purposes, this oonolusion is subjeot to the critique of further experiments involving more prolonged exposure of animals to somewhat lower concentrations. Properties of the Experimental Materials Aroolor 1242 is a light straw-oolored, mobile liquid with the composition of a ohlorinated biphenyl. A letter from mgns oaasi9 p.O. Benlgus, dated August 30, 1953* states that It oontalns 1*2.0 + 0 . 5 Pr oent of ohlorlne, which corresponds to the presenoe of 3 ohlorlne atoms. The positions of the chlorine atoms within the moleoule have not been determined. Monsanto Application Bulletin No. P-115* gives its specific gravity at 15*/25* as 1.373 to 1.333, and states that it has a distilla tion range of 325* to 3^0*0, a refraotlve index (D-llne at 20*0) of 1.627 to 1.629, a Saybolt Universal visooeity of lOO'F of 30 to 93 seoonds, and a flash point (Cleveland Open Cup) of 176* - l3o0. Its vapor pressure is about l* mm at 150C and about 30 mm at 200C. Although insoluble in water and glyoerlne, it is soluble in most organio substanoes. At 745 mm of sroury and 25*0, the oonoentration of 1 mg of the vapor of Aroelor 121*2 per liter of air is equivalent to 96.9 ppm by volume. Aroolor 1254, which corresponds to pentaohloroblphenyl, la a light straw-colored, vlsooua liquid. The positions of the ohlorlne atoms have not been establiehed. The moleoular weight of pentaohloroblphenyl is 326.1*45* of which 54.3 per oent ia ohlorlne. Benigus (ibid) reported that Aroolor 1254 oontalned 54.0 0,5 per oent chlorine. Bulletin No. P-115, states that Aroolor 1254 has a speoifio gravity at 25#/25#C of 1.533 to 1.543, a distillation range of 365* to 39"0, * refraotive Index (D-llne at 20*0) of 1.639 to 1.641, and a Saybolt Universal MUNS 080820 visoosity at 100*7 of l,f$00 to 2,500 seooada. The vapor pres sure at 150*0 la about 1*3 an and at 200*0 la about 9 am# It la soluble In most organio aubatanoea, but It la Insoluble In water and glyoerlne. At 745 am of meroury and 25*0 the oonoentration of l mg of Aroolor 1254 per liter la equivalent to 76.5 ppm by volume. Experimental Methods Exposure to the Vapor. In a preliminary experiment with the vapor of Aroolor 1242, a group of animals, consisting of a oat, 6 guinea pigs, 10 mioe, 4- rabbits and 10 rats, were oonflned for 7 hours on each of 5 days per week In a rectangular plywood ohamber (volume 600 liters), of whloh the inner metal lining was ooated with a baked ohemically resistant plastic, through whloh was passed a stream of air laden with Aroolor 1242 In a known oonoentration. The stream of air, conditioned with respeot to temperature (75 1 30? Inside the ohamber), dust and humidity, entered the ohamber through an "Anemostat," located at the oenter of Its top, at the rate of 500 liters per minute, as measured by an inollned manometer attaohed to a venturimeter (Figure A), The air was withdrawn by suotlon from the ohamber through an exit tube located on the rear wall near the floop, equidistant from the sides. MONS 0 0 3 0 2 1 -6- Aroolor 1242 was volatilized from a heated glass well main tained at 132* to 13<i*C, The air passed over the surfaoe of the liquid before entering the ohamber (Figure B ) . In a seoond similar experiment with the same Aroolor, the temperature of the liquid was kept at 100* to 105*C. l.hile the seoond experiment with Aroolor 1242 was in progress an experiment involving the vapor of Aroolor 1254 was oarried out in a seoond ohamber similar to the first exoept for the absenoe of a plastlo inner lining. The rate at whloh air flowed over the liquid Aroolor 1254, which was maintained at 130* - 135* vfas 400 liters per minute. In order to aooustom the animals to the experimental pro cedure, they were kept in their respeotlve ohambers for 7 hours on eaoh of 4 oonseoutlve dayB during the week prior to the in troduction of the Aroolor vapor into the ohamber. For purposes of oontrol, a third group of animals was oonflned throughout a Ilka period before and during the period of the exposure to the Aroolors in another ohamber supplied only with conditioned air. Method for the Determination of Aroolor 1242 or Aroolor 1254 in Air. These materials were determined quantitatively by virtue of the faot that, on thermal decomposition, they yield hydroohlorio aoid whioh, with silver nitrate, forms a suspension, the density of whloh oould be measured by means of the Beokman speotrophotometer at 500 iqu. HUNS 088822 On each day, 2 samples of air from eaoh respiratory ohamber were oolleoted by passing air at the rate of 1 liter per minute for 15 minutes through a Y.'illson oombustlon furnaoe, and then through 2 midget bubblers, in series, eaoh oontaining 10 ml of 0.1 N sodium hydroxide. A quartz tube (13 mm outside diameter, 7 mm inside diameter and 16 .25" in length) oontained several strips of folded platinum foil. The middle 9" of the tube was wrapped with a heating u n i t . The heating unit of the Vllllson furnaoe (Figure C) consisted of 20 feet of B. and S. gauge SS nlohrome wire (1 ohm per foot), and was oovered with asbeatoa oement. The sample of air was humidified by a ootton wiok saturated with water plaoed 0.5 lnoh upstream from the quartz tuba. With the furnaoe maintained at 50*0, the Aroolor was decomposed to free ohlorlne, which reacted v/lth the moisture to form HOI; this was absorbed in the 0.1 N sodium hydroxide in the midget bubblers (Figure 0) equipped vrlth frltted-glaee bubblers (Mine Safety Applianoe Company Ho. 4-3S67) . The 0.1 H sodium hydroxide waB prepared by dissolving 4.0 g of pellets (ACS speoifioations 0.01 per oent 01) in about 50 ml of double distilled water. To this was added 10 g of arsenio trioxide (ohloride-free) that had been dissolved In water and filtered through ' hatman No. 42 filter paper. Tha oomblned solutions were diluted to 1000 milliliters. mqns oaaa3 -a- The sample was transferred to a 25 ml graduated cylinder to whioh 1 drop of a solution of phenolphthalein was added (1 g of phenolphthalein dissolved in 100 ml of CP methanol). The solution was neutralized with 3N nitrio acid (190 ml CP ooaoentrated nitrio aold diluted to 1000 ml with double-distilled water) and diluted to 23 ml with double-distilled water. One ml of 3N nitrio aoid was added to obtain a pH of 1. After adding 1 ml of a solution of silver nitrate (3 grams of AgNOj diluted to 1000 ml with double-distilled water), the suspension was mixed by inversion. After standing 30 minutes, the trans mission of wave-length 500 mu was determined in a 50 mm oell by means of a Bookman speotrophotometer whioh was set against a corresponding oell containing a reagent blank. The amount of Aroolor was determined from a standardized ourve obtained in a similar manner from known quantities of sodium ohloride. A ourve relating the transmission at 500 mp of a suspension resulting from sodium ohloride over the range of 5.0 to 120 miorograms per 25 ml of final suspension is shown in Figure S. On the basis of 42.0 per oent of ohlorine in Aroolor 124-2 and 54.0 per oent in Aroolor 1254, 1 miorogram of sodium ohloride is equivalent to 1.4442 miorograms of Aroolor 1242 or to 1.1021$ miorograms of Aroolor 1254. mons oaaa2<* - 9- Experimental Result8 Aroolor 1242, Experiment No. 1. On oat, 6 guinea pigs, 10 nloe, 4- rabbits ana 10 rats survived when subjected for 7 Hours on eaoh of 17 days over the period of 2% days to the Inhalation of air bearing the vapor of Aroolor 1242 In the oonoentration of 3.6 miorograms per liter (0.33 ppm, Tables 1 and 5). No signs of intoxication were observed. The weights of the individual animals (oat, guinea pigs, rats and rabbits) have been plotted in Figures 1, 2, 5, 6 and 6, The averages end the ranges of the weights of guinea pigs, aloe, rats and rabbits have been plotted at semi-weekly inter vals in Figures 3, 4, 7 and 9, respectively. Despite slight fluctuations in their weight during the period of exposure, all of the animals, exoept the guinea pigs, gained in weight during the period of exposure (Table 6). The average gains made by the various speoies, expressed as per centage of the initial weights were: oats, 3.1; mioe, 10.3; rabbits, 77> and rats, 2.1 The loss in weight on the part of the guinea pigs averaged 2.0 per oent; most of them were gain ing weight (Figure 2) at the termination of the period of exposure. All of the animals were examined post-mortem, and since gross examination of the vlsoera did not reveal any signifi cant alterations, the tissues of only representative animals mqns oaaazs 10 - (1 oat, guinea pigs, 6 mice, 4- rabbits and 6 rats) were geotioned and examined mlorosoopioally. No abnormalities were found In the visoera of these animals. Aroolor 124-2. Experiment No. 2 (6.63 i ) /l), The pertinent data relating to the fate of the Individual animals In the Ex periment No. 2 are presented in Table 2 and summarized in Table 5. This group consisted originally of l oat, 6 guinea pigs, 10 mloe, 4 rabbits and 10 rats, but a few additional animals were used to replaoe those whioh died. One oat, 4 guinea pigs, 6 mloe, 2 rabbits and 6 rats survived throughout their exposure, for 7 hours on eaoh of 62 days over a period of 120 days, to air bearing the vapor of Aroolor 1242 In the concentration of 6,6} miorograms per liter (0.66 ppm). Certain animals (2 guinea pigs, 1 mouse, 1 rabbit and 1 rat) were killed after 65 to 66 periods of exposure for examination. In addition, 1 rat and 2 mloe survived throughout 23, 4l and 5 6 periods of exposure, re spectively. Seven fatalities occurred as follows: 1 guinea pig after 15 periods of exposure; 3 mloe after 24, 4l and 70 periods of exposure, respectively; 2 rabbits after 15 and 64 periods of exposure, respeotively; and 1 rat after 56 periods of exposure. Exoept in the oase of the rabbits, the inoidenoe of mortal ity among the oontrols was equally as great (or greater, Tables 4 and 5) as that among the experimental animals. One oat, 3 MUNS OOda^o - 11 lo, 3 guinea pigs, 3 rabbits and rats survived, following tbeir confinement, for 7 hours on eaoh of 84- days over a period of 122 days, in a chamber in which the air, conditioned with reapeot to dust, humidity and temperature, oontalned no vapor of either Aroolor. One guinea pig, 1 mouse and 1 rat were killed after 66 (or 68) periods of exposure. Other animals introduced later in the experiment survived throughout the following numbers of periods of exposure, respectively; 2 guinea pigs, 20 and 77; 3 mioe, 14-, 14 and 19 ; 1 rabbit, 19; and 1 rat, 44.Xleven fatalities ocourred at stated times among the oontrol animals, as follows: 2 guinea pigs, after 7 and 6o periods, respeotlvely; 7 mioe after 5 59, 68, 69, 70, 72 and 82 periods, respectively; and 1 rat and 1 rabbit after 42 and 65 periods, respectively. No uniform or suggestive signs of intoxication were ob served among either the experimental or oontrol groups. The weights of the individual animals (oat, guinea pigs, rats and rabbits) exposed to Aroolor 1242 are shown in Figures 10, 11, 12, 13, l6 and l8. The average weight, and the great est and least weights attained by any individual within the groups of guinea pigs, mioe, rats and rabbits, are plotted in Figures 13, 14, 17 and 19, respectively. Comparable data on the oontrola are given In Figures 31 to 4l. mons oaaa7 - 12 - The surviving experimental animals grew equally as well |or better) as the oontrols (Table 6). During the period of exposure to Aroolor 124-2 (6.83 / ) /l), the average gains, ex pressed as percentages of the Initial weights, by the various animals \ere as follows: oat, 22.2; guinea pig, 56.4-; mloe 21.2; rsbblts, 505; and rats, 17.6. The comparable findings on the oontrols were: oat, 6,6; guinea pigs, 36.6; mioe, 17 .4-; rabbits, 4A.5; and rats, 16.6. The weights of the livers and kidneys and the relation ships of their v/elghts to the body weights (expressed as grams per 100 grans of body weight) of the Individual animals that survived following exposure to the vapor of Aroolor 124-2, are shown in Table 7, wbloh also gives tbs corresponding data on the oontrols. Comparison by the T ' test of the varianoes of the ratloe of the liver or kidneys to the body weights of rats, guinea pigs and rabbits exposed to tbs vapor of Aroolor 124-2 (6,83 Y / l ) , with those of tbs oontrols, revealed no slgnifleant dlfferenoes (P *0,05) exoept In the oase of the livers of the rats. Application of the "t" test to the mean values (Table 6) revealed no significant dlfferenoes between the test and oontrol groups of guinea pigs, rats and rabbits. The apparent prothrombin oontent of the blood, as measured by tbs method of Kato (Am. 7. Clin. Path., 10:14-7, 1940), of m o n s oasa^a 13 rtalfi animals that had been exposed to the vapor of Aroolor 1248, are given In Table 9* Comparable findings on the oontrols are presented In Table 10 to Table XS the percentile relationships of the olotting power have been obtained arbi trarily by dividing 100 times the average olotting time of the blood of the control animals by the olotting time of the blood of tbe experimental animals on the same day. No diminution in the olotting power of the blood, as a measure of the impairment of the funotion of the liver, was induced by the exposure of the animals to the vapor of Aroolor 124-2 when present In the air In the oonoantratlon of 6.6 mlorograms per liter. Determinations of the numbers of erythrocytes and lsuoooytea and of the hemoglobin oontent to the peripheral blood of indi vidual guinea pigs and rabbits subjeoted to inhalation of the vapor of Aroolor 1242 to the oonoentratlon of 6 ,6 mlorograms par liter, are given In Table 12, whioh also includes oomparable results on oontrol animals. These values have been summarized to Table 13. Application of the "t" test to differences in the mean values (Table 14) for the experimental and oontrol animals yielded border-line evidenoe of significant differenoes to the numbers of leuoooytea end In the concentration to the hemoglobin to the blood of the guinea pigs, but these were small and of opposite sign (the leuoooytea in the blood of the test animals MGNS 08 8829 - 14 - era low In number while the hemoglobin content was high) , as *11 as being subject to individual variation, and they oannot be regarded as of physiologioal signifioanoe. Of the 7 animals that died during the seoond experiment with Aroolor 12*12, all but 2 aloe were examined post-mortem. The tissues of 2 mioe had undergone significant changes, post-mortem, and were not examined microscopically; gross evldenoe to determine the cause of death was not available. A third mouse ( B - 2 W was examined and found to have a malignant lymphoma that involved the liver, spleen, lddneys and heart, and therefore its death was attri buted to natural oause. The cause of the death of the guinea pig was uncertain, but the 2 rabbits and the rat were found to have died of pneumonia, and not, apparently, of chemical pneu monitis. Generally, the survivors among the various speoies had normal visoera. However, 2 guinea pigs and 1 rat had degenera tive lesions in the liver and lddneys, 1 guinea pig had aoute lobular pneumonia, and 1 rat had Interstitial pneumonia. Eleven of the animals (2 guinea pigs, 7 mioe, 1 rabbit and 1 rat), that had been confined in conditioned air only, died. The vlaoera of 6 of these (1 guinea pig, 4- mioe and 1 rabbit) were examined miorosoopioally. Each of the animala examined had died with pneumonia, in association with degenerative MUMS 086030 - 15 - clang of varying severity in the train, liver and kidneys. Animals of all speoies that survived were examined and found to have normal visoera, but for soattered pneumonio lesions in oertain of the guinea pigs and mloe, and for one oase of oellu- litis in a rabbit. Aroolor 1254-, Experiment No. l (5.40/ / I ) . The pertinent data relating to the ohange in weight and the fate of each in dividual animal during and following prolonged intermittent exposure to the vapor of Aroolor 1254- are given in Table 3 and re summarized in Table 3. The original group consisted of tha same numbers and types of animals employed in the other experi ments. One oat, 3 guinea pigs, 4 mice, 2 rabbits, and 9 rats survived throughout their exposure, for 7 hours on eaoh of 3 days over a period of 121 days, to air containing Aroolor 1254 in the concentration of 5*40 mlorograms per liter (o.4l ppm). Oertain other enimals (3 guinea pigs, 6 mice, 1 rabbit and 1 rat) were killed for examination after 66 or more (up to 74) periods of exposure. One rabbit, introduced after the experi ment had bean under way for some time, survived, having under gone 33 periods of exposure. Five enimals died during the experiment, 2 guinea pigs after 12 and 17 periods o f exposure, respectively, and 3 rabbits after 15, 15 ad 20 periods of ex posure. Exoept for tha rabbita, the incidence of mortality among the exposed and oontrol groups was comparable (Tables 4 and 5) No signs of intoxioatlon were noted among the experimental animals. mcns oaaan The weights of the individual animals (oat, guinea pigs, rati end rabbits) are given In Figures 80, 21, 28, 25, 26, 26 end 29, while the average weights of the guinea pigs, mice, rats and rabbita are shown in Figures 23, 24, 27 and 30, respectively. Eroept in the oase of the guinea pigs and rats, the animals that survived throughout the period of exposure to the vapor of roolor 1254 grew equally as well as the controls (Table 6). During the period of exposure the gains made by the animals of various apeoies, expressed as percentages of their initial weights, were as follows: oat, 22.1 ; guinea pigs, 40.7; mloe, 6.9; rabbits, 39*7; and rats, 6.9. The lesser gain by the experimental rats may be explained by the faot that they were initially somewhat heavier. The corresponding values for the oostrol animals have been given previously and are also shown in Table 6. The weights of the livers and kidneys, and the ratios of these to the body weights, of the animals that survived through out the exposure are shown in Table 7 Application of the "t" test to the differences in the mean values which characterized the experimental and control animals (Table 6) shows that the weights of the livers of the exposed rats were slgnifioantly greater than those of the controls, the ratio of the liver to the body weight of the former being 53*i 8 per 100 g of body weight, that of the latter being 4.15'g/100 grams. MGNS Otitidic: - 17 - The results on the apparent prothrombin oontent or the blood of rabbits and of a oat, following exposure to the vapor of Aroolor 1854- (5-to *7l) ere given In Table 9. Little or no diminution In the clotting power was induoed by exposure to the vapor of Aroolor 1254- (Table 11). The numbers of the oellular elements and the oonoentration of the hemoglobin in the peripheral blood of the Individual animals subjeoted to Inhalation of the vapor of Aroolor 125b are shown in Table 18 and are summarised In Table 13. The slight apparent lnoreases In the hemoglobin of the guinea pigs and In the numbers of erythrooytes of the rabbits, while statisti cally significant (Table lb-), oannot be taken seriously as ex pressions of the effeots of the inhalation of Aroolor 185b. The visoera of b of the 5 animals (8 guinea pigs and 3 rabbits) that died after varying periods of exposure to the vapor of Aroolor 125b, exhibited diffuse degenerative ohanges in the brain, liver and kidneys, in association with pneumonia* Animals that survived, following exposure to the Aroolor, were examined post-mortem, and the visoera from representative ani mals were examined miorosoopio&lly. The visoera of the oat and guinea pigs were normal. All of the mloe, exoept one, had normal visoera; the exoeption (B-837) had slight degenerative lesions in the liver. A rabbit killed 1 day after the 66th MQNS 0 8 8 8 3 3 - id - p*rlo4 of exposure, had degeneration of the liver and kidneye, nil pulmonary hyperemia and edema* Otherwise, the rabbits that M r* examined had essentially normal viscera. All of the rats that were examined had degenerative ohanges in the liver, and la 5 of the 10, these changes were found to be associated with pneumonia of low grade. The Aroolors did not appear to aot as primary pulmonary irritants. The pneumonia that was observed in both the experlnental and control animals had the oharaoteristios of an in fectious rather than a chemioal prooess. The toxio degenera tive ohanges which were found in the liver and kidneys of all speoies of animals, in both experimental and oontrol groups, while not associated with pneumonia in all instances, may well heve owed ttieir presenoe to the interourrent disease vdiioh was characterized, ohlefly, I n m o s t Instances as pneumonia. Dlsousslon The probability of the ooourrenoe of the oancentratione of vapor maintained In these experiments in aotual industrial praotioe is remote, slnoe these values approaoh those of atmosphere saturated with the Aroolors* (The signifloanoe of partioulate matter, in the form of oondensed droplets or of pro duo ts of ther mal decomposition, has not been involved in these experiments*) mons oaaaj4 - 19 Sinoe the animals which were killed for examination after if to periods of exposure were found to have degenerative lesions in their visoera whioh oould not, with certainty, be attributed to extra-experimental oauses, these experiments ware terminated and others have been initiated at about 1/3 to l A the concentration From The Kettering Laboratory in the Department of Pre ventive Uedioine and Industrial Health, College of Medlolne. University of Cinoinnati, Cincinnati, Ohio* Project No* 47 Experimental Yiork and Report by: Joseph F. Treon, Ph.D# Frank P. Cleveland, H.D. John Oappel, B,S. Frederio E. Shaffer, M.S. Ralph Vi. Atohley, B.S. John P. Torbeok, B.S* June 2, 1935 Direotor HONS 086835 Table 1 The Mortality, Length of Survival, and Changes In Weight of Animals Exposed to the Vapor of Aroclor 12iiZ In Air (Experiment No. 1) Concent ration V A ppm 8.6 0.85 Duration of 'Exposure Identification (hours) Number Initial Weight (Mr ) 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 X 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 X 7.0 17 X 7.0 Cat A-l.1.6 2.865 Onlnea Pig B-351 -- Quinea Pig B-332 Guinea Pit, B-333 0 ^ 6 0 Guinea Pig E-336 Guinea Pig B-335 o M Guinea Pig B-55o 0.685 House B-251 Mouse B-252 Mouse B-253 Mouse B-256 Mouse B-253 Mouse B-256 Mouse B-257 Mouse B-250 Mouse B-259 <j: 25-- 0.028 0.027 0.026 0.021 0.021 0.021 0.028 0.019 Mouse B-260 0.025 Rabbit I-37U Rabbit 1-375 "O o o 6.180 Change In '"eight Expressed as Percentage of Initial Weight During After Period Period of of Exposure Exposure + 8.1 + 0 .2 - 10.lt- + 1.2 ; |:is - 23-.07 : Si - 5.0 - 2.8 - 1.5 1.9 *+ i3.-6fi + - 3.8 3-6 0.0 0.0 + 7-7 + 19-0 - 3.8 - 6.8 + 19.0 - 160 *A:l + 10.5 21.7 3.0 8.6 - 17".l 13-9 5*8 Length of Sur vival After Final Period Fate . of Exposure Survived Killed 17 days-- Survived Killed 17 days Survived Killed 17 days Survived Killed 3 days Survived Killed 3 days Survived Killed 10 days Survived Killed 10 flaynSurvived Killed 10 days Survived Killed 17 days Survived Killed 17 days Survived Killed 17 days Survived Killed 3 days Survived Killed 10 days Survived Killed 3 days Survived Killed 10 days Survived Killed 17 days Survived Killed 17 davs Survived Killed 3 days Survived Killed 10 days 90 0800 SNOW Table 1 - Pane 2 Concent ration V71 ppm 8.6 O .83 Duration of Exposure . Identification (hours) Humber ********** OOOOOOOOOO 17 x 7.0 Rabbit I-376 17 x 7.0___ Rabbit I-XT7 Rat Rat L-I4.37 Rat L-438 Rat L-li39 Rat L--ijij.0 Rat L-ljia Rat L-kk2 Rat L-kki Rat T.-li/11 Rat L-W+5 Change in Weight Expressed as Percentage of Initial Weight During After Initial Period Period Weight of of (kg) Exposure Exposure Fate 1^.225 A.kOO 0.227 0.250 0 .25^. 0.207 0.228 0.250 0.240 0.230 0.238 0.25k 2.9 * 10.* + 1.8 + + k1.-2k + 0.5 + 2.2 4- 0.8 + U .6 2.2 4+. 21*.03 4- 1.8 * 6.8 4- 2.6 4. 0.8 : Vk + O.k + 5.6 + 1-7 ; Si 5-9 Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Killed 17 days Killed 2k davs Killed 3 days Killed 3 day8 Killed 10 days Killed 10 days Killed 17 days Killed 10 days Killed 17 days Killed 17 days Killed 17 days Killed 17 days MQNS 08883 7 Table 2 The Mortality. Length of Survival, and Changes In height of Anlamla Exposed to the Vapor of Aroclor 12U2 In Air (Experiment Ho. 2) Concentration Z S _E 0.66 Duration of Exposure Identification (hours )_______ Humber JS-UL 7.0 7.0 7.0 7.0 7.0 7.0 i27 J x 77i.0Q o x 7.0 li x 7.0 2li x 7.0 82 x 7.0 82 x 7.0 82 x 7.0 la X 7.0 Cat A-liiiS Guinea Pig B-325 Guinea Pig B-326 Guinea Pig B-327 Guinea Pig B-32 Guinea Pig B-329 Guinea Pig B-330 Guinea Pig B-h.19 House B-21 House B-2i|2 House B-2l|3 House E--2iilv Mouse B-2l(5 Mouse B-2l|0 Mouse B-2l|7 Mouse B-2ij Initial Weight 0) 2.2-i O.W 0 00I l k 0 0.1 0.020 0.02k O .023 O.OI7 O.OI9 0.020 0.021 0.019 Change in Weight Expressed as Percentage of Initial Weight During After Period Period of of Exposure BxpoaursI Fate 22.2 106.7 + 57-0 8.7 U5-3 + - 12.5 U3 23-5 + 31.6 + 10.0 + + 20.1 0.8 9.5 Survived Survived Died Survived Survived Survived Survived Survived Survived Died Survived Died Survived Survived Survived Died Length of Sur vival After Final Period of Exposure Killed 1 day Killed 1 day 1 day Killed 1 day Killed 15 days Killed Killed 115 day days Killed 1 day Killed 1 day 1 day Killed 1 day During 2i|th per iod of exposure Killed 1 day Killed 1 day Killed 15 days During (|lst per- __ lod of exposure mun oaaaaa 6e08fiO SNOW Concentration V/l ppm 6.8} 0.66 Duration of Exposure Identification (hours) Number Initial Weight (kg) Change In Weight Expressed as Percentage of Initial Weight During After Period Period of of Exposure Exposure Fate 82 x 7.0 82 x 7.0 58 x 7.0 111 x 7.0 82"x 7.3-- 82 x 7.0 6k x 7.0 66 x 7.0 15 x 7.0 Mouse B-2k9 Mouse B-250 Mouse B-376 Mouse B-u.ok Rabbit 1-570 Rabbit 1-371 Rabbit 1-372 Rabbit 1-373 Rabbit L-96k 0.020 + 35.O 0.020 + 25.0 0.02k 0.02k * - 5 : | --- T & j? ~ 2.600 + 59*<> + 51*0 2.585 2.k90 20.5 + k o .8 2.758 + 2.2 + 5.0 0.0 + ik'k Survived Survived Survived Survived Survived Survived Died Survived Died K x T- 'O 82 x 7.0 82 x 7*0 82 x 7.0 56 x 7.0 82 x 7.0 82 x 7.0 82 x 7.0 82 x 7.0 82 x 7.0 23 x 7.0 Rat L-526------ --- 0,220 Rat L-527 Ret L-528 0.219 0.200 Rat L-529 Rat L-53O 0.202 0.213 Rat L-53I Rat L-532 00..210963 Rat L-533 0.219 Rat L-53k Rat Rat LL--853356 0.227 0.228 0.207 i 9-1 + + 2157-.5O : in + lk.o + lk.l + 19.6 + 17.2 i 'S.I 3.2 + k .8 + k .8 Survived Survived Survived Survived Died Survived Survived Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Killed 15 days Killed 15 days Killed 1 day Killed I das--Killed 1 day Killed 17 days 1 day Killed 1 day During 15th per iod of exposure Killed 1 day Killed 1 day Killed 1 day Killed 1 day 1 day Killed 1 day Killed 8 days Killed 15 days Killed 15 days Killed 15 days Killed 1 day Tabi 3 The Mortality. Length of Survival, and Chances In Walgfat of Animals Exposed to the Vapor of Arcelor 12Su In Air (Experiment No. 1) Concentration 3 2 PPm iiko OM. Duration of Exposure Identification (hours) Humber Initial Weight 0 ) 825_x' r.o 7*0" 83 83 * x 77-.0o 15 X 7-0 Cat A-UUU_______ SulneaMg Guinea Pig B-32 Guinea Pig B-321 Guinea Pig B-322 2.320 00 ..11++0020 O.36O 12 x 7-0 66 x 7.0 x 7-0 X-.7.Q 7k x 7-0 71+ x 7.0 83 x 7.0 I k x 7-0 71+ x 7.0 8} x 7.0 6b x 7.0 71+ x 7*0 83 x 7.0 Guinea Pig B-323 Guinea Pig B-32+ Guinea Pig B-l+lj flulaaft-P la B-U18 House B-23I House B-232 House B-233 Mouse B-232+ Mouse B-235 House B-236 House B-237 Mouse B-238 House B-239 0O 0.022 0.020 0.02+ 00..002109 0.019 0.020 0.019 0.022 Change In height Expressed as Percentage of Initial Weight During Affter Period Period of of Exposure Expoeurel _ 2 2 .1 wrr * 47.3 47.01..51 TH+ 11.9 - 23.0 + 31.4- + fi!.! + * 55.0 37.5 31*6 39-0 26.3 + 2351..10 9.1 U-5 Fate Survived Survived Survived Survived Died Died Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Killed 1 dav Killed l6 days Killed 16 days Killed 1 day During 15th per iod of exposure 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 13 days CHflffflO SNOW Table 6 - Pag 2 Duration of Concent ration Exposure Identification r/i ppm (hours) Number 5.4.0 0.4l 8636 * x 7.0 83 x 7.0 83 x 7.0 15 x 7-0 15 x 7.0 20 x 7.0 33 x 7.0 o3 66 x x 77..00 83 x 7-0 83 x 7.0 83 X 7.0 83 x 7.0 83 X 7-0 83 x 7-0 83 x 7.0 83 X 7.0 Moose B-240 Rabbit 1-362 Rabbit I-363 Rabbit I-364 Rabbit 1-453 Rabbit 1-587 Rabbit I-J18 Rabbit 1-887 Rat L-299 Rat L -517 Rat L-518 Rat L-519 Rat L-520 Rat L-521 Rat L-522 Rat L-523 Rat L -524 Rat D-525 Initial Weight (kg) 0.022 2.993 l.'S c 2.729 2.100 6.22k O .235 0.217 0.22k O0..22337I 0.235 0.226 0.2^2 0.200 Change in '"eitht Expressed as Percentage of Initial "elRht During After Period Period of of Exposure Exposure Fate + 23*2 - 0.0 + 38.3 + 1.6 1i 1 : 1 + 36.7 *. j + 2.1 - 1.3 + 10.6 X 4.0 12.8 13.5 - 2.1 :U + 5.0 Survived Survived Survived Survived Died Died Died Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Killed 16 davs Killed 1 day Killed 1 day tilled 16 days 2 days 3 days 1 day Killed 1 dav Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 15 days Killed 15 days Killed 15 days Killed 15 days m o n s oaaa4 Tabla k The Mortality. Length of Survival, and Change In Weight nf Anlmala Exspeed to Conditioned Air Containing Mo Vapor nf nf Aroclor 12ii2 or Aroclor 12SU (Experiment Mo* 1) Duration of Exposure (hours H i ) 8|i x 7.0 5 */?.o 66 y 7.0 814. i 7.0 J8L/ x x 77-.00 60 x 7.0 77 x 7.0 20 x 7.0 8L x 7-0 68 x 7.0 68 x 7.0 72 x 7.0 82 x 7.0 Identification Number Initial Weight (kE )(l) Change In Weight Expressed as Percentage of Initial Weight During After Period Period of of Exposure (1 ) Exposure Pate Cat A-k3<i Guinea Pig B -313 Guinea Pig B-3I4 Guinea Pig B-315 Guinea Pig B-3I6 Guinea Pig B-317 Guinea Pig B-3I0 2.388 0.335 0.43i850 S:S 0.4-U+ 6.6 + + 9255..72 + - 768..37 + 4- 7270-.31 4 15 .5 4114.5 Survived Survived Survived Survived Died Survived Died Guinea Pig B-I4O2 Mouse B-221 Mouse B-222 Mouse B-223 Mouse B-22h O.I4.69 OJiVi 0.021 0.021 0.022 0.021 44 53.5 29.2 4 U 4..3 4 23.8 4 9.1 0.0 - - 4 .3 Survived Survived 'Survived Survived Died Died Mouse B-223 0.021 0.0 - Died Length of Survlval After Final Period of Exposure Unless Otherwise Stated Killed 1 dav Killed 1 day Killed 1 day Killed 15 days 1 day Killed 15 days During 60th period of exposure Killed 1 day Killed 1 dav Killed 15 days Killed 1 day 1 day During 72nd period of exposure During 82nd perloc ---- of. ftXBogure---- ?4G8R0 SNOW f<?09BO SNOW Duration of Exposure (hours Xl) 69 x 7-0 59 x 7-0 70 x 7.0 8k x 7.0 8k x 7.0 5 * 7.o Ik x 7.0 lk x 7.0 19 x 7.0 Bt x 7.0 8k x 7-0 814. x 7.0 65 x 7-0 19 x 7.0 gk x 7.0 8k x 7-0 8k x 7-0 8k x 7.0 I42 x 7-0 8k x 7.0 8k x 7.0 Identification Buaber Mouse B-226 Mouse B-227 Mouse B-228 Mouse B-229 Mouse B-230 House B-k99 Mouse B-531 Mouse B-532 Mouse B-555 Rabbit I-365 Rabbit I-366 Rabbit I-368 Rabbit I-369 Rabbit 1-795 Rat L-567 Rat L-508 Rat L-6O9 Rat L-510 Rat L-511 Rat L-512 Rat L-513 Initial Weight (IwXl) Change in Weight Expressed as Percentage of Initial Weight During After Period Period of of Exposure!1] Exposure Pate 0.021 0.0 0.022 0.022 . - kp*9 - ll-5 0.023 0.023 0.023 0.023 0.015 0.050 3.08(4 3.162 2.568 5*352 3O.I4 0.0 - + 8kk.-36 - 26.7 + M|.B.l6 5P-7 lk.O 2.2k} O.2 9 0.231 0.212 0.225 0.212 + fj+.B + 2.2 -r 9-9 + 19.1 - 3k-k 0.192 27.6 0.203 4 26.6 - - 0.0 0.0 - 0.0 0.0 0.0 -18.0 -rmi-- 3-9 3-8 -0.9 0.0 * 1-5 Died Died Died Survived Survived Died Survived Survived Survived Survived Survived Survived Died Survived Survived Survived Survived Survived Died Survived Survived Length of Sur vival After Pinal Period of Exposure Unless Otherwise Stated 3 days 1 day During 70th period of exposure Killed 3 <*ais Killed 3 days 3 days Killed 3 days Killed 3 days Killed 5 davs Killed 1 day Killed 1 day Killed 15 days During 65th period of exposure Killed 1 day E H l e d `'l'5 clays---Killed 15 days Killed 15 days Killed 15 days During k2nd period of exposure Killed 3 days Killed 3 days Table U - Page 3 Duration of Exposure (hours )(1 ) 8k x 7.0 8k x 7.0 x 7.0 kk * 7-0 Identification Number Rat L-5lk Rat L-515 Rat L-516 Rat L-7k8 Initial Weight (*g) O.I95 0.217 0.210 0.218 Change In Weight Expressed as Percentage of Initial Weight During After Period Period of of Exposure(l) Exposure 12.8 2k.k 232-.93 0.0 0.0 0.0 Pate Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Unless otherwise Stated Killed 3 days Killed 3 days Killed 1 day Killed 3 days (1) Computed from time of Initial exposure of experimental animals to the vapor of Aroolor; the original group of controls and the original group of experimental animals were confined -into conditioned air only, on each of four days during the previous week- rr909O SNOW Tabla *> of the Data on Mortality Among Groupa of Experimental and Control Animals Material Concent ration W1 ppm Aroclor 12l)2 8.60 O .83 Duration of Exposure (hours) 17 x 7*0 Number of Fatalitles/Humber of Animals Exposed Cats 0/1 Guinea Flics Mice 0/6 0/10 Rabbits Rats o A 0/10 Aroclor 12l)2 6.83 0.66 82 x 7.0 0/1 1/7 U ) (2 ) 3/12(1) 2/5 U ) 1/11 (1 )(4 ) (3) Aroclor 1254 5.40 0.4l 83 x 7-0 0/1 2/8 (1 ) (2 ) (5) 0/10(1 ) (6) 3 / 7 U H 7 ) 0/10 (1 ) Controls 0 0 84 x 7.0 0/1 2/8 (1 ) (8) 7/14(2) 1/5(10) l A K D ( l i ) . -JL-, (1) / One survivor was exposed on only 65 or 66 days. (2) ( One survivor was exposed on only 67 or 66 days. / Two survivors were exposed on only 4l and 58 days, respectively. One survivor was exposed on only 23 days. (5J One survivor was exposed on only 71 days. (6 ) Five survivors were exposed on only 74 days. (7) One survivor was exposed on only 33 days. (8) Two survivors were exposed on only 20 and 77 days, respectively. (9 ) Three survivors were exposed on only 1I4. and 19 days, respectively. 10) One of the survivors was exposed on only 19 days, ll) One of the survivors was exposed on only 1)4 days. S * r 9 9 9 0 SNOW table 6 The Average Change In Weight of Experimental and Control Animala Survivors Animals That Died oecles n Average Change Average In height at Weight Average Termination During Initial of Height Experiment Exposure (1 ) Peg) P*g>______ ___ t t e l __ Average Change In Weight Expressed as Percentage Of Initial Weight (1) n Average Average Change Change in Weight Average in Expressed Weight at Weight as Average Termination During Percentage Initial of Exposure of Weight Experiment (1 ) Initial (fcg>___ fcs) ___ (g) Weight (1) Group Exposed to 8.6V o f Aroclor 121^2/liter (Exp. Ho. 1) Eat I - '"27HSr~ " T L W K ----- Guinea, . Pista 6 Klee i<5 0.6b9 .2b 0.6L.0 .26 Rabbits b b.l95 Bata lo 0.258 .2U3 0.2*2 " *""8 .1---- T T " - 0.008 0.02 + 0.I9I + 0.005 - 2.0 + + + 72*.17 0 _ - - - - -- ---- Group Exposed to 6.83 V f Aroclor 12J|2/llter (Exp. Ho. 2) Cal: ~ T ~ Guinea ,rlPlKS 6 jjiee $ Rfcbifcs 5 Rats io 2.271 o.bob .21 2.687 .2i ~ s : t k ------ + O . W L T "2272---- 1 T " 0.629 0.225 .26 + .a. 5.898' - + 1.5'l ~ 6 ; k 5------ + 0.050 _5_6.b____ l + 21.2 + + 5F? 17.6 23 1 - 0.585 .o2o 27872"" .215 - 0.521 5.019 27588 0.200 -- - 0.06b - 16.6 '- .Ol - :____ 0 . ______ - 0.015 - .i MONS 0 8 0 8 * 6 i Table 6 - Page 2 Survivors Animals That Died Average Average Average Change Average Change Change in Height Change in Height Average in Expressed Average in Expressed Weight at Weight as Weight at Height ae Average T e n d nation During Percentage Average Terminatioi During Percentage Initial of Weight Experiment Exposure of U ) Initial Initial of Exposure of Weight Experiment (1 ) Initial Species n (kg) (kg) ___(kg) Weight (1) n (kg) (kg) (kg) Weight (1) Cat i 2.32 Guinea Pigs 6 Vice 16 Rabbits L 0.U09 0.021 T TfS T Rate I T " 0.227 Grout Exposed to 5 . U V o f Aroclor 125ii/liter <055 22.1 --- 0.571 6. 5.b6b 0.2ki 0.162 + 0.O6 .&S + 0.0I5 Up.7 + 25a + 39./ + tmi 2 0.U02 0.277 0- 3 27*v59 27153 0 - Gr oup Confined in Conditioned Air Only --- - 0.125 - 32.1 - - 0.U16 - i7.li -- CIS I - Guinea Pigs 6 Vice Rabbits A Rate 1 77BB-- 0.U18 (T.052 2.76U 0.21S - r i m ----- + . K 7 o .b a l k .02 0.2L 6 + 0.227 + .02 + 1 .2U.B 6.5L 6.6 + 56.6 4 lV.il + u u . + 16 .u -- -" 2 b 00..032929 o0-.te2s0 - 0.050 .1 _____ - Z6i.1 ___________ 1 ]-352 5.022 0.1x70 i r a s 1 0.22 o -iil - 0.75 - S - u : ( 1 ) Pinal Welftht - Initial Height x lOOg C O ---- initial height' " >8880 SNOW Tabi 7 The Relationship of the Weights of Certain Organa to the Body Weights of Animala Exposed to the Vapor of Aroclorl2h2 or Aroclor lZ5h Liver Kidneys Identifi cation Number Ratio of Weight of Number of Body Perioda of Weight Organ x 100 to Body Exposure l k ) . _ B ___ Weight Ratio of Weight of Organ x 100 to Body JS___ Weight Aroelor 121+2 6.83 Mlorograns/llter Cat -149 --- 82----- W Guinea Big B-325 82 639 Guinea Fig B-527 82 798 Guinea Big B-328 82 609 Guinea Fig B-529 66 102 Guinea Pig B-330 82 698 Guinea Pig B-U19 Average 67 7) $5 bkO Rabbit 1-370 02 1**5 Rabbit Rabbit tI--Ji 731__ Average 82 66 77 1+300 390b C2? 92. 23.9 31.U 31.0 22.0 39.0 3L..0 29.6 112.0 4k-g lio.fl 122 Iff 3730 w ' .631------ 3.7U 34 3.93 . . _ if-? 5.09 6.0 0.532 0.61k 0-98? 5.3U ____ 9.01 if>5_ 1.092 6.2 0.888 jit.?8 i.D 2753 3f.0? 4-5 ik_______ .Oh Tlfot*f6: p6..781079 0.3h0 .50 6.969 O.libo Identifi cation Number Tabla 7 - Page 2 Liver Number of Body Periods of Height Exposure _jLsl___ _ s____ Ratio of Weight of Organ x 100. to Body Weight Kidneys ,.s.. Ratio of Weight of Organ x 100 to Body Welrht 6 3 B 9 9 0 SNOW Cat A-filili Guinea Pig B-519 Guinea Pig B-320 Guinea Pig B-321 Guinea Pig B-32li Guinea Pig B-U15 .. 5 z m _ 83 966 83 61i0 . 83 66 682 510 ____11_____ li62 93.3 --- 372?------- nrrc --------- 22.9 li.05 li.6 0.813 27.3 26.3 26.0 -22:?-, il.27 3.86 5-10 5.02 5.2 .. 0.813 lt-2 li.8 Jti___ 0.6l6 0.9U 0.866 Identifi cation Number Table 7 - Pag 3 Liver Kidneys Number of Body Periods of ''eight Exposure _JjlL___ ___g ... Ratio of Ratio of Weight of Weight of Organ x 100 Organ x 100 to Body to Body Weight _ s ____ Weight OSPPP0 SNOW '" - [ ------ BE------ )? & T -- f-LB .;' -| ' "1 Y 9 I.........f ITT,!! " |"p;?gg T S B P 8 0 SNOW Identifi cation Number Number of Periods of Eaposure Liver Body 'eight Ratio of Weight of Organ I 100 to Body U ) - __ s__ Weight Controls 8 Ratio of Weight of Organ x 100 to Body Weight Table 7 - Pag S Liver Kidneys Identifi cation Number Rat L-qiZf Rat x R a t __H__E_ Rat L-5V) Rat Lhafc xJrkU Average- Number of Periods of Exposure Body Weight Ratio of Weight of Organ x 100 to Body __ g_____ Weight Controls ~87T 10.5 ~T77?~ 270 2?61s7r ~W T -g fg72~ H2T3T" 1 2 .J - 70 Ratio of Weight of Organ x 100 to Body Weight ~ Q $ 8'' 1 E 0.70k 0.807 Aroclor 12I|2121+2 121+2 125U 125U 1254 121+2 12k 2 121+2 125U 125U 125U 112255kU 125U 125U 1254 125k Liver Liver Liver Liver Liver Liver Kidney Kidney Kidney Kidney Kidney Kidney Liver Liver Liver Kidney Kidney Kidney Speclee n Rat Guinea Pig l Rabbit Rat 103 Guinea Pig 6 Rabbit 1+ Rat 9 Guinea Pig 6 Rabbit Rat 105 Guinea Pig 6 Rabbit Rat 10u Guinea Pig 'b Rabbit Rat 10k Guinea Pig Rabbit k Ratio or Average Average 'eight of Organ Body Organ x 100 'Velght Weight to Body --.iil--1172 -J2ia+oil- Weight 29.6 61+0 122.0 12.8 12250..09 1.85 1+023 239 581 325q5O? 3-0i+ 5*3l+ I+.51 3*53 0.773 5.0 l8.6 2.1 k .6 15.8 9.0 10502..33 2.0 1+.8 19-5 61+0 1+023 253891 3537 5993 66^ 3995 *aos 0.1+68 0.770 0^06 0.1+61+ {+.15 l+*5l 2.61 0.807 O.73I 0.501+ t 1.2381 0.1+291 1.5085 03*.03090902 1.0122 0.581+2 00..17+215777 0.1+199 O.5I4. U 0.3163 - - - - - p -- 0720-0.30 ^0.50 0.10-0.20 <0.01 > 0.50 >0.50 > 0.50 0.1+0-0.50 - > 0.50 > 0.50 > 0.50 > 0.50 - - 8 S8 8 8 0 SNOW t rx - i2 nJnaTn + na - 2) \ (nx + n s){Sx| + Sig) df = Di + na - 2 HUNS a8 8 S< Date 9/11/55 10m/2Ui/33 11/ 1I1/53 12/5/53, WA Pa "Prothrombin Tin"; (Method of Koto) or the Blood of Anlwala Following their Expoaurt to the Vapor or Aroclor 12U2 or Aroclor IZ^li In Air Clotting Time, Seconds Experiment Ho. 2 Aroclor 1242 - 6.83 Y j \ Cat A-445 20.00 18.50 18.00 16.50 17.00 19.00 17.00 jEm : 19.00 17.00 13.50 .00 .00 16.050 Rabbits -- --37.L 19.50 17.00 15.00 15.00 13.50 15.00 17.00 15.00 1-57^ 19.50 18.00 1143..5500 13.50 16.00 ~J=3T5~ 18.50 16.50 13.50 1 4 .0 0 14.00 16.75 16.75 MCNS 088855 Date 9/26A 3 10/24/53 11/1V53 m 1/26/54 Tbl <> - p a Cat A-444 20.00 20.00 16.50 16.50 17.00 20.00 20.00 1-362 17.00 16.00 16.00 15.00 15.00 17.25 Clotting Time. Seconds Experiment No. 1 Aroclor 1254 - 5 *U0 ^'/l 1-563 18.00 16.50 16.00 14.00 111.00 16.50 Rabbits 1-564 17.00 18.00 15.00 15.00 1 3.50 16.50 1-453 17.00 17.00 18.25 14.00 1-718 14.OO 15.00 15.00 -- 1=58=7 16.50 15.OO rttm"frotiiro,bi n T "- * t | , i t t l , , < J <*1' r ^ t n ) o f t t v . r ir > c K l o r r o llo w ll T n .lr C o n fln w n t In C u t e o u d d I l o t ? wltn CondlU on^d A i r C u n u ln lim Mo V i p o r o t A ro clo r 12U2 or A roclop 12SU Clotting Tine, Seconda Date 9/11/53 9/12/53 9/26A3 10/24/03 11/14/53 12/5/53, m in k Cat A-k35 20.00 20.00 16.25 13.75 17.25 13.50 19.75 18.00 I-3 bb 16.00 16.00 15.25 13.50 14.50 SO 16.75 15.50 1-566 16.00 16.50 13.50 14 .50 13.00 16.25 15 .50 16.00 Rabbits 1-568 16.00 16.00 15.50 14.50 13.50 15-75 17.75 1154..0205 1-369 1168..2550 1154..0705 13.00 17.00 1-975 1147..0205 Average 16.63 16.19 13.50 16.13 16.81 1154..0904 9SP0PO SNOW The Effect of the Ex p or Aroclor lZSh ln~A lod of Several Months, Upon ' T M 11 Relation of "Prothrombin Time" of Control and Exposed Animals Expressed Arbitrarily in Terms of Percentage Change in Clotting Time of Blood 100 x Clotting Time of-Blood of Control Animals Clotting Time of Blood of Exposed Animals Experiment No. 2 Aroclor 12i|2 - 6.8; f/l Time of Sampling in .Relation to Period of Eiposnre Days Before First Period Serial riumber of Per iod After Which Blood Was Drawn TtL 11 - *> i- Relation of "Prothrombin Time" of Control and Exposed Animals Expressed Arbitrarily In Terms of Percentage Change In Clotting Time of Blood 100 x Clotting Time of Blood of Control Animals______ Clotting Time of Blood of Exposed Animals Tima of Sampling In Relation to Period of Exposure Days Before First Period Serial Number of Perlod After Which Blood Was Drawn Experiment No. 1 Aroolor 125I4. - 5*4 ''71 Cat A-lilJi 100.0 100.0 98.5 113.6 101.5 92.5 90.0 1-362 10971..28 89.1 95-9 90.0 93-5 1-363 9928..31 89.1 102.7 96.L 10970..15* Rabbits I-i6U 1-453 97.8 89.9 95.0 97*8 95-2 78.1 95.9 100.0 97.8 100.0 T-5H7 1-710 1-007 102.7 ' 96.1* 81.8 112.1 Average B 90.0 99.6 102.0 112.1 97.0 95-4 95-2 100.0 12 109 15 30 ?2 k5 03 (2 ) PSflfifiO SNDW (2) Determination made lit- days after 83rd period of exposure Table 12 The Effects of the Intermittent Inhalation of the Vapor of Aroclor 12L2 or Aroclor 12.!. or of Conditioned Air, tlpon the numbers of Erythrocytes and Leucocytes anJ~lTpbn the Concentrm- tlon of Hemoglobin In the Peripheral Blood of Animals Experiment Ho. 2 Aroclor 121^2 - 6 .8 3 V/l: 7 hours/day Identifica tion Number Cat A-U45 Dates of First and Last Period of Exposure 9/14/53 1/11/54 Guinea Pig B-325 9/14/53 i/n /54 Guinea Pig B-326 9/14/53 10/2/*v5 'Guinea Pig -j27 9/14/53 ' 1/11/54 Date of Determi nation 12/22/53 Average 9/18/53 10/29/53 12/21/53 Average 9/ 16/53 109//1269//5533 12/21/53 Average Hemoglobin g/100 ml 12.4 10.3 10.0 10.9 13-8 S3 14.5 15.8 14.6 15.8 15.8 15.4 Erythrocytes (Thousands) per mri5 8 ,0S0 7 ,18 7,180 7,470 5,480 6,540 6,o4o 6.010 5,830 5,26 6,620 6,030 5,970 Leucocyte* per a 3 20,950 12,700 15,050 16,218 16,350 10,300 11,200 l2 617_____ 16,750 6,400 11,300 8,200 8 ,6 3 3 -- 6 S8 8 8 0 SNOW 09B9R0 SNOW Table XZ - p 2 E xp erim ent Ho. 2 r o c lo r 121*2 - 6 . 8 37^ 1 ; 7 hcmre/dmy Identifica tion Number Guinea tig 6-328 Dates of First and Last Period of Exposure 17W 753~ Guinea Pig B-32 1/11 M 9/14/53 gigne5-rag~B=53D" 1 2 /1 5 / 5 3 9/14/53 Guinea Pig 6-419 Rabbit 1-170----- 1 /1 1 / 5 4 16/5753-- 1/11/54 9/14/53 1/11/54 Date of Determi nation 9/16/53 10/29/53 12/5/53 Average 9/16/53 1 0 /2 9 / 5 3 12/12/53 Average 9/18/53 10/29/53 12/21/53 Average 12/21/53 9/16/53 10/28/53 1 2 /2 2 / 5 3 Average Hemoglobin g/100 ml . 12.7 13-1 13.4 13-1 15 .0 13-1 l4.2 14.1 12 .0 15.4 13 .8 13-7 14.2 1 1 .6 13.4 12.7 12 .6 Erythrocytes (Thousands) per m m 3 5,290 5.550 5 ,6 2 0 5.487 5.150 5.500 5,420 5.357 4,930 6,370 5,740 5 .6 8 0 5,570 5.T80 6 ,90 0 5,590 6 ,0 9 0 Leucocytes per mm3 lg,30 8.150 15,850 10,767 5,700 9,150 11,950 5,60 0 6 ,5 0 0 8 ,3 0 0 10 ,0 0 0 8 .2 6 7 8 ,15 0 9.40 0 15,40 0 10 ,60 0 11,8 0 0 T9RB90 SNOW Tabi iz Experiment No* 2 Aroclor 121|2 - 6.83 v 7 l: 7 houra/Gay Identifica tion Number Dates of Plrat and Last Period of Exposure Late of Determi nation Rabbit I-3?l---Rabbit 1-372 9/14/53 - 9/16/53 1120//2282//5533 l/U/54 Average 9/14/53 Rabbit 1-373 Rabbit 1-964 12/11/53 9/14/53 12/15/53 12/15/53 1/7/54 Avarafe 9A6/53 10/28/53 12/9/53 Averaee 12/22/53 Hemoglobin g/100 ml 12.0 11.4 12.4 11.9 11.6 13.1 12.4 11.6 11.6 13.1 12.1 11.6 Erythrocytes (Thousands^ per mm? 5,900 5,940 6,030 5,957 5,970 7,090 . . 6.530 56,,5o44o 5,l4o 5.573 6,290 Cat A-UUk Experiment Ho. 1 Aroclor 1254 - 5*40 V 7 l : 7 hours/day 9/14/53 9/16/53 11.6 9,540 Leucocytes per an? 12,250 12,300 7,350 10,633 _ 8,950 14,000 _ 1_14T5_. 8,150 !0,450 8,600 9,067 5,800 25,900 MONS 088862 Tbl \ 2 - PK. k Experim ent Ho. 1 A ro clo r 125k - 5.1^0^71: 7 hours/day Identifica tion Humber Cat A - lM Cates of First and Last Period of Exposure Guinea Pig B-319 1/12/5* 9/1U/53 Date of Determi nation 10/30/53 12/23/53 Average Guinea Pig B-320 Guinea Pig B-321 Guinea Pig B-322 Guinea Pig B-323 1/ 12/ 5* 12/22/53 Averase 9/1V53 9/21/53 1121//22/25/533 1/12/5* Average 9/1V53 1/13/5* 9/21/53 H/2/53 12/22/53 Averaee 9/1V53 10/2/53 9/21/53 9/11/53 | 9/29/53___ 9/21/53 Hemoglobin g/100 ml. 12.0 13-U 12.3 15.0 15.0 13 * lk.5 lit..6 15.8 13.8 lU.7 114.. 114..6 15.* Ik.9 6 13-* 15.0 Erythrocytes (Thousands) per mP 87350 7.550 8.k93 5,780 5.970 5,560 5.777 5,760 5,690 5,310 5,587 5,*50 6,190 6,170 5.937 5,760 6,270 Leucocytes per rmP 25,100 26,350 25.783 16,000 9,550 11,350 12.300 12,100 8,550 16,550 12,k00 166,,63550 12,100 11.700 lk,950 8,700 T*ble 12 * Par. ^ I? Experiment No. 1 Aroclor 125li- - ^'U-OV^l; 7 tiours/day Identifica tion Nnmher Guinea Pig B-524 Gates of First and Last Period of Gxnosure *9/14/53 " Guinea Pig B-I4.I5 Guinea Pig B-I4.I8 12/15/53 9/30/53 1/12/54 10/5/53 Rabbit 1-362 1/12/54 --- 9755753 Rabbit I-363 12/15/53 --- 9/55753" Gate of Oetermi- natlon 9/21/53 1121//212//5533 AvAT*AA mb Avnraee 11/2/53 12/22/53 AveraRe 9/18/53 10/30/53 12/9/53 Averace 9/18/2 1/12/54 Average Hemorlobin g/100 ml 15.0 13.8 14.2 14.3 14.2 14.6 14.4 14*6 14.2 14.4 14.2 13.4 13.1 13.6 1113.-64 11.6 12.2 Erythrocytes (Thousands) per mm 3 5,880 6,200 6,110 6.061 5,930 5,710 S .820 6,330 5,410 5,870 6,850 6,020 5,960 __----------------6,770 5,960 5,470 6,067 Leucocytes per mm3 15,200 15.167 22.900 12.900 17 A Q 2 . 10,000 10,600 10.300 15,300 12,400 13,100 13.600 7,400 10,450 12,500 10,117 C 9 B B 8 0 SNOW i HONS 088S6<* Identifica tion Number Rabbit I-3&4 Rabbit 1-453 Rabbit I-587 Rabbit 1-718 "Rabbit I-887 Exparlnsnt Ho. X Aroclor 125I1 - 5.1*0V"/l, Dates or First and Last Period of Exposure 9/1 4 /5 3 1/12/54 9/14/53 10/2/53 10/5/53 .10/23/53 10726/53 11/20/53 H / 23/53 1/12/54 Date of Determi nation 9/18/53 10/30/53 12/23/53 Average 9/18/53 10/30/53 12/23/53 Hemoglobin g/100 ml 1123..44 12.4 12.7 15.0 11.6 15.8 y hours/iUy Erythrocytes (Thousands) per nn? 66,,84930 6,300 6.sko 6,900 6,500 6,630 c a r -435 ------ 578753-- 1/ 13/54 Conditioned Air 9/22/53 11/ 3/53 12/23/53 Average 10.0 10.6 11.0 10.5 7,970 7,140 7,200 7,437 Leucocytes per mm* 6,050 13,800 10,000 11,050 10,850 9,500 25,050 14,450 12,350 17,283 MONS G 8 8 8 & 5 Identifica tion Humber Guinea Pig B-313 Dates of First and Last Period of Exposure 9/3/53" . 1/ 13/54 Guinea Pig B -314 --- 978753" 12/15/54 Guinea Pig B-3l5 -- 5/8/53 1/13/54 Guinea Pig B -Jlb -- 95/728275/533 Guinea Pig B-317 9/8/53 1/13/54 Date of Determi nation 9/23/53 H/4/53 12/23/53 Average 9/23/53 11/4/53 12/12/53 Average 9/23/53 11/4/53 12/23/53 Average Hemoglobin g/100 ml 13.4 13.8 15.4 14.2 12.0 14.2 -- 13.1 3.3.8 I4.6 14.2 14.2 Erythrocytes (Thousands) per mm? 56.,04890 5,870 | j j j Leucocytes per mm? 11,250 15,050 11,150 ___ ^ 8 1 3 _________ 5,29 5,780 5,230 12,453_ 12,15 14,800 19,250 5.433_________ 15,40Q-- 5,790 5,510 5,540 14,950 17,050 17,050 5,il3_________ ______ 15,867 _ 9/23/53 1121//42/35/533 Average ! t | 1153..04 15.0 14.5 56,,998700 6,590 6,513 6,000 105,,44550 7,300 990PPO SNOW Table 12 - Page B Conditioned Air Identifica tion Humber Guinea Pig B-3ICJ Dates of First and Last Period of ExDOsure i/m i-- 12/U/53 Guinea Pig B-4-02 " 9/23/53 Date of Determi nation 9/23/53 liA/53 Average Guinea Pig B-576 1 /13/54 12/ 14/53 Rabbit I-365 1/13/54 --- 9/5/53--- 12/23/53 Average 12/23/53 Rabbit 1-366 1/13/54 9/8/53 1/13/54 12/23/53 Average 9/22/53 12^23^53 Average Hemoglobin g/100 ml. 13 A 12.7 13.1 1135-.81 15 A 1I1 . 1 A .2 ii.li 12 A 13.U 12 A 11A 1 1 .U 12.7 11.6 Erythrocytes (Thousands) per mm3 5,520 5,320 5,1*20 5,320 5,21 5,830 5,1*53 5,860 5,900 5,800 6,200 5.967 5,860 5,760 5,280 5,633 leucocytes per nan3 9,900 17,000 13,1*50 10,100 10,650 12,150 10,967 9,i*oo 8,900 8,100 11,1*50 9,1*83 16,400 16,250 13,750 _ 15,1*67 Identifica tion Number Rabbit I-368 Rabbit 1-369 Rabbit 1-975 Dates of First and Last Period of Exposure 9/0/53 1/13/5* 975/53 12/14/5* 12/ 15/53 1/13/5* Table 12 - Page 9 Conditioned Air Date of Determi nation 9/22/53 11/3/53 12/23/53 Arerage 9/22/53 H/3/53 12/9/53 Average 12/23/53 Hemoglobin g/100 ml 1 1 .1*. 1121..44 . u7 1122..04 11.6 12.0 13.1 Erythrocytes (Thousands) per m n a 5,630 5,060 6,240 5,643 6,490 5,900 5,260 5,883 6,420 Leucocytes per mm3 9,100 10,900 13,100 11,033 11,700 9,100 16,300 12,367 9,600 TM > 1* The Avartae Wuyberi of ErTthroCTtya and L a u c o c H and the Average Concentration of HewoiXobln In the Peripheral Blocd of Experimental and Control Unltnala Erythrocytes : 3 Thousands per mm Leucocyte*: per mm3 Hemoglobin : g /100 ml Species of Animals Average X n Average X n Average X Experiment No. 2 Aroclor 121+2 - 6.83 V/l. : 7 hours/day n Guinea Pigs Rabbits Guinea Pigs Rabbits 3 7 7 S ---------- 7 b !o88 _________ 5____________ __ Li o , n (il| _ 7 ----------5 __________ ------------ 1112+..11+'T2)'- Experiment No. 1 Aroclor 125l+ - 5.1+0 V/l. : 7 hours/day -------- 7 _______ 5_________ T 7BBS------ --- 8------ 6.1+86(2 ) 6 1102.^82171 0 6 ------ llt-5 (2 ).. 13.2______ ---- 8 6 ulnea Pigs X Rabbits C2l 5,729 5,909 Controls Exposed to Conditioned Air ? ------ 127125:-- 7 5 11,5 9 0 5 13 .9 12 .2 7 5 (1) Value significantly less than that obtained on controls. (2) Value significantly greater than that obtained on controls P9BBB0 SNOW A p p lic a t io n o f the " t " T est t o the HwmtolQKlctl Data Obtained In dbaervat^ons on Exposed and d o n tro l Anl'malB Species of Animals Erythrocytes thousands /mm3 df I t 1 r Leucocytes Per mm df | t 1 F Experiment No. 2 Aroclor 12l*2 - 6.83 v/l; Hemoglobin 6/100 ml dr J ___ ______ 1__ 7 hours/day P Guinea Pigs T2-- -- 0.T601T >0.5 " 12-- 2.789!u0.0i-0.02'T2-- T 3 5 5 f Rabbits 8 0.8298 0.1x0-0.50 8 1.1x57110.10-0.20 8 0 .5 0 8 6 Experiment No. 1 Aroclor 125I4. - 5 .4-0 Y J1; 7 bours/day -- <75701-- _______ Guinea #igs I 13 Rabbits I9 753TT7-- STJT^Tr Tj--- 2TI75I-------- ,0.02-ii705' 0 .61x5 7 1 >0 .8 0 9 I1 .61x25 0.20-0.10 t = xx~ x s n^nz (nx + ng - 2y . df = m + n z - 2 (1 + 112)(Sx| + Sx|) 699890 SNOW 1 J 8.6 Mlorograma of Aroolor 12l(2/llter Figure 1 MUN 08B870 mows o a a a 7 i Time, Weeks ',,r.i-or'jimnt Mo. 1 .6 Mlorograma of Aroclor 1242/liter Figure 3 MON i U d Mloe B2 5 I to B20 29 28 27 26 25 21 23 22 21 20 19 18 17 16 - Ported of -- * _____ Exposure____________________ Time, Weeks xper'irent No. 1 .0 Micrograms ofF Aigruoor elor4. 121+2/llter mons oaaa?3 nati W eight, Grams 8.6 Micrograms of Aroclor 12l+2/lltor Figure 5 MONS 088874 t.(> Mlcrograma of AroeXor X2l*2/Xlter Figur* 6 MONS oaaa?5 Tims, Weska ipe-lmBi t No.l 8.6 Mlerograms of Aroclor 12l*2/llter Figure 7 MQNS 0 8 8 8 7 6 Weight L^oe.rlrcent. No, 1 8.6 Micrograms of Aroclor 12l4.2/llter Figure 9 MUNS 0 8 8 8 7 8 R O lg h t , Drams Time, Weeks Experiment No. 2 fc.B Mlcrogrnms of Arcelor 12l42/lltor Figure 10 MONS 080679 Weighty Grama '.xonrim . tit. N o. 2 6 .8 M lorog ram a o f A ro c lo r 1 2 lj2 / lit e r F ig u re 11 mons oaaaao / 6,8 Micrograms of Aroclor 12l|2/llter Figure 12 MNS O l IT il- : fe Time, Weeks experiment No. 2 6.8 Mlerogrnraa of Aroolor 121+2/llter Figure 13 MONS 08888 W eight, Gmras Mice BkUl to B250 end B376, B1+61+ ------------------ Period of------------ J 33 "T Exposure --------- 1 32 / . t\ l ,k A31 33 ' 11 JL /3 29 28 n \ r\ i , Kl V r V A / M ' ik 27 26 / v r 1 ........ { \ . _ __,____ A ,i. A K 25 2k . i j .r 'A..-r i /\ i 23 22 7^+7 . K/ i iA ______ Lki________________ i. i./ \I A! 21 20 fi / i \ A | |! j \l____ ________ \. , .) \ A ' ii ' 19 v A t 18 y v 1 17 16 15 ll+ 13 Time, Weeks xnoi'liMSrit No. 2 fe.B Micrograms of Aroclor 12l*2/llter Figure ll+ mons uaaaaj r 1 253 'felght, Grama Timo, Weeks ..j'n.'rMcr.t Ho. 2 6.8 Micrograms of Aroclor 12142/llter Figure 15 mun Qdaaa" i it I W eight, Grama T im e , V/eeka "i'xporlraont. N o, 2 6.U Micrograms of Aroclor 12U2/lltor F ig u re l MONS 0tt885 Rata L5 2 6 to L535 and LR36 320 310 300 290 200 2?0 260 25O 2I4O 23O 220 210 200 I90 100 UmB, Vseles ".xj-.er'.raint No. 2 6.0 Mlcregrama o Aroolor 12i*2/llter Figure 17 MONS Ujoo 00 teoo teoo 3900 Rabblta I37O to 1373 and 196!+ h T\/ . _______ J :z S.??T0!l | --33070000 t-------------------- ^ ----------------- _it - i 36OO _, _ ,333135+000000 1 ------------------------------ 1" --------------------------/ ---------P e r io d o E xp o su re f -------- 1 ------- ------------------J - ------------------------------------------- i - i ------------ yoo ^ 3100 (~ 3000 f- ?oo Weight, Graos oaaaa7 V e lg h t f Cromi 1000 l+l+oo R a b b its 1 3 7 0 t o I 3 7 3 and I 9 6 I* U300 1+230 1*130 1*000 3900 3800 3700 3600 3500 3l*oo 3300 3200 3100 3000 , /( 7\ 2900 / 2800 2700 2600 1/1/ ,r 2500 21*00 ; U V 1 .A-- H y- K /r, /A j / -k "' 1- - I ( /' i /! / ! / /V /r /r 1 / T / F p \1 ' P Tims, Weeks Sxnerimunt No. 2 6 .8 Nllcrograms of Aroolor 12l+2/llter Figure 19 I MUNS a d d i Weight, Grans Tine, Weeks Experiment, No . 1 5-4 Mlorogrnns of Aroclor 1254/llter Figure 20 MQNS 0dadd9 W eight, Ox*runs xperiment No. 1 T im e , W eeks 5-U Mtorogrnme of Aroclor Figure 21 MONS 0809 Weight* G r ma >.pe rim a n t N o . 1 T im e , W eeks 5.1+ Mlorogrems of Aroclor 125l+/llter Figuro ZZ Hons oaaa9 i 800 700 G u ln o n P i g s B 3 1 9 t o B 3 2 I4. n n d B ' ' n d B l+ l8 1 _> r ------- 6 oo I a i^OO t - j'" ' to .y 300 ________ . 1 . o r ^ - H 7' 1 / -- 11 \j ^ t ^ i- + + + " ' s. 200 -- ----------- \___ P e rio d o f E xposure ----------------- ;-------------- - Time, Weeks xporircent No. 1 5 U Mlorogrsms of Aroclor 125Vllfcor Figure 23 moms oaoa'iz Timo, Weoka 'Tperinierit Ni. 1 5 .1+ Mlcrogrnms of Aroclor l^l^/lHar Figure 2l| M NS 0bb93 R ats L299 and L 5 I 7 to L520 / 250 \\-- 225 A s/ " , t \ 1 I --1 T 1 1 K llle 3 200 250 225 200 225 200 300 2?5 250 22S 200 M -- r H - l --i-'t-- -- f' L'5 1 7 -- (" '- ( -- ' A -- 1 * i~ K ille d . _____________________________ 1 .- 4 _______ 1 K ille d ' 275 250 225 200 T/ K ille d E xposure Time, Weeks zporlaorit No. 1 5 .It Micrograms of Aroclor 125h/llter Figure 25 MUNS U0da94 250 i__________ Rta.k521..t o L525___________ 225 200 L521 K lllu d 275 250 22S -| 20? Klllud g 53 2? 230 ito 175 300 * 275 250 225 250 225 200 7^ 175 Period oE3tpo3ure L525 Killed 1- Time, YYeeka :..rpcrlmont No. 1 5 -It- Mlcrogrnras of Aroclor 125lt/U-ter Figure 26 HONS 088895 W e ig h t, G rana 5 .I1 M lc ro g rn m s o f A ro o lo r 1 2 5 i( /llfc 0 r F ig u re 27 HONS 0 8 8 8 9 6 W e ig h t, Groms 1*200 1*100 1*000 3900 3800 3700 3600 3500 31*00 3300 3200 3100 3000 2900 2800 2700 2600 2300 T im e , Weeks ' ixp e rim e n t No. 1 5.1+ M ic ro g ra m s o f A r o c lo r 1 2 5 l * / l l t e r F ig u re 28 mons oaaa97 Weight, Grama 21+00 2300 2200 2100 2000 1900 1800 1700 1600 1500 li+00 1300 900 2800 27OO 2600 2213+0000 3000 2900 2800 22760000 2900 2000 2700 2600 2215+O0O0 2300 2200 2100 Rabbits 11+53, 1587,1718, and 1887 \l 1K ' \ \ 1Died ... ; '1 1,mfl7 1 Died / ' \1718 vDled .-H-rr _l 11i 1i-rn-1887 Killed S / yn ( / __________________ ..... - ------ Period of Exposure ---- -* Time, Weeks irperimont Ho. 1 5 .1+ Micrograma of Aroclor 125l+/llter Figure 29 MOMS Ud89a Weight, Grams 411**213030300 3900 3803 3700 3600 3300 3343-3003 3200 3100 3000 2900 2800 2700 2600 2300 2400 2300 2200 2100 R a b b its 1362 to I3 b 4 and 1453. 1718. nnd 1887 , \/ >y ____________LZ yy '/ L ,/t / --- 1 / l/ 1 ! [T V '/ / 7* k -t r LJi r\ - n__ /___________ 3 1 1587 V. vr s 2000 1900 1800 1700 1600 " P e rio d o f E xposure . Tima, Weeks T:xrrIrr.ant No.l 5.4 Mloroernns of Aroolor 1254/llter Figure 30 MONS 088899 Weight, Grams Time, Weeks C o n tro l S u b je c te d to C o n d itio n e d n x p e r i m e n t B o . 2 A r o c l o r 121+2 a n d l lx p a r ir a e n t B o . 1 A r o c l o r 12^1+ fig u r e 31 A ir; fo r MQNS 0B890U W e ig h t, G rans T im e , Weeks C o n tro ls S u b je c te d to C o n d itio n e d E x p e r im e n t N o . 2 A r o c l o r 121+2 a nd x p e rlm e n t N o. 1 A ro c lo r 125U F ig u re 52 A ir; fo r MONS 083901 '''xperlment No. 1 Aroclor Figure JJ MUN 08a90<> Time, Weeks Controls Subjected to Conditioned Air; for Ljcperirer.fc No. 2 Arcelor 12i2 and Experiment No. 1 Aroolor 125U Figure 34 MOMS 0Stf90J 30 ZS 28 27 26 25 24 23 * 22 21 20 19 18 Time, Weeks Controle Subjeoted to Conditioned Alr;for txperiment No. 2 Aroclor 121+2 and IApeilment-No. 1 Aroolor I25I4. Figure 35 MNS 008904 iV e lg h t, Grams 2i3 225 200 250 225 7 200 250 225 200 275 250 225 200 v -"H -h l\ y ___________ LJ-y R a ts L 507 to L512 4-- r L537 K ille d -- -- -- __ (--- ____________ L _ ^ ~ - 4 1 1 L$D8 'V r r L K llle d K' 1 `'' t . i , _________ -L 509 K ille d --________________ -4-- "1 1----- 1- L 510 J C llle d 2^0 225 200 175 150 275 250 225 200 V1 1\ \ LS11 D ie d i^ V + " r r T ^ . 1 1 U -'K . 512 K ille d 175 E xposure T im e , Weeks C o n tro ls S u b je o te d to C o n d itio n e d Ox i-h r i n e n t N o . 2 A r o c l o r 12l;2 a n d E x p e r im e n t N o . 1 A r o c l o r I25U Figure 36 A ir; fo r m<JNS 088905 1 Tima, Weeks Controls lubjested. to Conditioned Air; for ~7.r>eri,nen`t No. 2 Aroolor 121^2 end 1x^erircent Ho. 1 Aroolor 125i| Figure 38 MGNS 060907 Weight, frema Figure 39 MOMS 0a90a Weight, Grams Rabbits 1368, 1369, and 1975 1+000 -r- ^ `""UtB " "Killed 3800 j-r. V' 1 ........ . y r 1+600 T T F ___________________ Jn' __ .-V. 1+1+00 j L _________________L l _________ 1+200 ^A rp t ^ ______ \________ i+000 -7 ^ \1 5800 1369 I _____ __ Died 3600 l/' 3600 31+00 3200 3000 2800 2600 31+00 \ i rj 5200 ------------ 3000 2800 Y A 2600 21+00 / /< z p,srlofi of soKpoaure 1975 ' i y i X-Lw u . '\/ 2200 C r\ on xp t o ro ls rim e . n s t u b je No, c te 2 d A Tritoomcel,Co oWnedeikt iso r 121+2 ned and A ir; f or D x n e rlm ? n L ;N o . 1 A rFo icgluo rre 1218+51+ MUNS 088909 Weight, Grams 'Time, Weeks _ Controls Subjected.to.Condi Cloned Air; for 'xp<;rlment No. 2 Aroolor 121+2 and 'xnarlment No. 1 Aroolor I2 5 I+ figure 1+1 MOMS 0 8 8 9 1 0