Document G5j07zGemZ64D310kgVGMVEKN

t r\. t~ ^ . r.' f / *. i: . >'-'j C*. J .LEAL COUPAMIHAPIOH OP MEZA"VALLBX Lead mobilization with-Ca-Na^-EDSA from the body of inhabitar Pusan Djuric,Ljubica Graovae-leposavi<5,Svetislav Milie and Ljuba Sonicar Institute of Occupational and Radiological Health,Bel grade, and Medical Center,Ravne, Yugoslavia She paper is dedicated to prof.Lr.J.Peisinger, Director of the Institute of Coeupation&l Ileal th in Prague,Czechoslovakia, for his 70-th birthday. In the Meza valley,Slovenia,Yugoslavia, a considerable lead contamination exists, due to pollution by lead aerosols from smelting plant in Zerjav. Pairing in consideration that the smelting plant is reconstructed in 1896, we can assume that, the population is Increasingly exposed to load until today. Prom 1967 a broad ecologic study on lead content of air, water, soil, plants, food and lead exposure of population in ^ Shis paper Is based on work performed under contract BSD-Ci-YiC between Institute of Occupational and Radiological Health, hoigrade and US Public Health Service. N11682 2 : the valley has "been developed. So find out the lead body burden we performed mobili zation of lead using Ca-Nag-EDIA in' 108 inhabitants, mostly woman and children, non-professionally exposed to lead. Methods and material A portion of the urine was collected from 108 persons, living in contaminated area, who are not professionally expo sed to lead. The plastic bottles were specially decontaminated on lead. Then 5 cem of CahagEDTA solution(TK5alcium-Dek" ) con taining 1 gr. of this agent, was injected intravenously to the adults. Half of the dosis is injected to the children. After that 24-hour urine sample was. collected. ' Determination of lead in the urine, after destruction of lead chelate, was performed by polarographic method and ex pressed inmibrograms per liter. Results and discussion. : Recently many authors performed lead mobilization in professionally non-exposed persons, using various chelating agents, to establish the body burden. The lead body burden depends on many factors as nutri tion, geographic position, social level etc. Therefore, hero for comparesion we can use data about lead mobilization per formed on Yugoslavs or on a population with a similar 'way of life, for example Czechoslovaks. In Yugoslavia Stankovic (1) studied lead excretion EPI p005G3 after oral application of CaEa^EBSA in printers without c.lin:L- : cal symptoms of lead poisoning. He established that spontane ous lead excretion, before chelating agent application was until 0,120 mg per h. sample of urine. After application of " . j, 3 gr. of Mosatil per os excretion was 0,236 ^ 0,101 mg,Pb/24 h. Stanlcovic accepted 0,340 mg Pb/24 as upper limit of mobiliza tion yield in non-exposed persons. Peisinger.et al. (2,5*4) studied 50 healthy, profes' sionally non-exposed persons and established an average sponta neous lead excretion as 0,032 mg. and 0*100 mg/24 h. as upper normal limit. After one injection of CaEDTA lead excretion va ried from 0,062 to 0,390 mg/24 h. Eherefore Seisinger recon- V *' mended 0,350 mg/24 h, of lead as upper limit for mobilization yield. In the other group of non-exposed persons he observed values, until 0,500 mg/h. in a fraction of the urine as up per limits We divided the studied group in the various subgroups following lead excretion after CaNe^EDSA injection mobilization yield as presented in fable 1. Average lead excretion before (spontaneous) and after CaEhSA injection is presented on 'fable l * V. * ..,* J. U <*!.. . X J II , .1 ., ' "gi. * ... vj . i. ! . *. . . j v. W. . ,. n All persons showed spontaneous lead excretion under 1 - 1 . * I V * I ... ^ # *.* i'l V ...... f i \* -I : * . . 0,100 mg/l(average 0,411 mg/1). . ` t i .\J. , o 5 i. j - , * O , /. . - . ` . v We accepted Eeisinger*s criteria for mobilization yield of: non-exposed persons. In that case only 5,5 CA of the persons L t 1 ^ I ' V I ' .1. , * . ` > I l , v. I '. *1 . I , excreted until 0,350 mg/1 of lb after mobilization, and 13,5 p V t . i i. ...* . o .<./- ( . -* j excreted the upper normal limit until 0,500 mg/1.; Ehe other l'i > V EPI 000504 81,5 excreted 0,5-4,2 mg/1 of lead, 2hat means, in spite of quite "normal" spontaneous excretion of lead, after mobilizati 81.5 CA persons showed increased mobilization yield, due to ab normal lead body burden. It is of interest to consider the relation of spontane . \/ ously excreted lead to mobilized lead. Pryerovska (5) studied, this relation in professionally non-exposed and exposed person In non-exposed persons spontaneous lead excretion represent 15.5 -- 1,9 of the quantity excreted in the first 24 hours after 1 CaEDPA injection. In the exposed, persons this relation was; 6,4 - 1,4 feisinger and Sr6ova (2) performed similar investigation and established this relation as 21-28 $ in non- -exposed group and 10-155& in exposed workers. We calculated the relation of spontaneously excreted lead to mobilized .lead for mentioned subgroups. Phese relation presented on gable 5. In the first two groups with "normal lead mobilization yield" these relations are about 7,5 - 5,02,3 In persons showing abnormal lead mobilization yield this rela tion is between 4-1*5. Mentioned results are suggesting that, this case represent a special situation where persons are ehro nically exposed to higher environmental lead level from birth day until death and for many generations. Phat means, their body burden is similar to that of exposed workers, but biolo gically active lead (8) portion is much smaller. Teisinger and al. (6,7) established on rabbits that after one CaEKDA injection about 21-27/0 of the lead body har den (deposit) is excreted in the urine during first 24 h. in EPI 000505 acute poisoning. In the case of chronic exposure of animals this relation i-. much smaller and only 79 5 of deposited lead is excreted. If we assume that these animal data could he extrapo lated and applied on human beings, then we can expect that in chronically exposed persons about 8 /o of the total lead body burden will be excreted after one CaEDlA injection. Shis hy pothesis is allowing us to suppose inhabitants of Meza valley contain lead body burden from normal (4-5 mg) to about 10 ti mes higher -52,5 mg. IVe performed a broad study on this group of population Including medical examination, many biochemical, toxicological and hematological analysis, subjective complaints. All these results are in course of the evaluation to find out if any pa thologic effects appear due to increased exposure to lead through the life time and for many generations in the Meza val ley.. Such studies could give some very Intersting ansvjers connected with the problem 'of the city population exposed to lead contamination due the vehicle exhaustive gases. EPI 000506 gpx 000507 t aoH N5 H r--Hi O a -Hap> 0$ CM W a> Pchf pq O EH ' o rH -0P) OH <1) "rdt a> r-i 0bO) ri U<D 4 rH vO$>X d -P rHm ' o d- EH *p H p pi d d o1 jd d p rl o p p o d o dd o rl rH .$ d EH 0 n rfi O P OJ Od W Sd Q d \o i-l p rH 0 d -p K\ a P pd W & j =j . a ct f>j OJ rH cu d 3 rl d. o rd i0 CO *P p 0 dP oo ft K ca H o d o rl P d (fH o M O o OJ l o o o CVI . \v ^ LA 0'3** Q. +1 LA 'LAm - rH O' 00"Vv 4' " ' rH OJ i o +1 o o rH rH IA VoOx ia CA 0* c a OJ CA ! +! o o UN o% . . CA CA : *1* o LA IA XOOX CN#*.. LA +! V L A# <A CA IA rH H p d p d 0 >i -P ,0 d N cn -h ft rH d 'H 0 ,0 Po OG OJ o IA +! LAm IN d H 0 hO dd o -p 4r3l dQ) do rH Pi oo w ft - R E P E KEN-CEB : : 1. Stankovic H..,Petrovic Ly, ,Poleti j.: A Contribution to the Leber; tory Diagnostics of Parly Batumi sinus ,Arh.hrg.rada & Toskikol* 12.: 189-194,1962 2. Teisinger J. ,Srbova J.:Diagnosticks olova pomoci CaEDTA,Prac. 1 ek, 10:402-406,1958 3. Teisinger J. ,Srbova J.: The Value of Mobilization of lead by Cab: in the Diagnosis of Lead Poisoning,BritJ.industr.Ked. 16:146-15. > 1959 b 4# Teisinger J. :Zkusenosti s ambulantnim vysetrovanim diagnosticke mobilizace olova,Prac.Lek. 16:145-147,1964,No.4 5. Prerovska I. :Yztah spontanne vylucovaneho olova v moci a olova vylucbvane^o po CalTa^EDTA,Prac.Lek. 21:361-564,1969,No.8 6. Te singer J.,Prerovska I.,SedivecV. :Stanoveni depozita olova v tele v eksperimentalni otravy olovem,Prac.Lek. 19:251-256,196-7,N* 7. Teisinger j. ,Prerovska I. ,Sedivec V. ,Elek J.,Eoth Z,: Attempt on Determination of Biologically Active Lead in Organism in Expori; tal Poisoning,Intern.Arch.Gewerbepath.Gewerbehyg. 2^: 240-255 > 19; 8. Prerovska I., Teisinger J. :Excretion of lead and its biological Activity after Termination of Exposure,Brit.J.industr.Med. 22:552-355,1970 EPI 000510