Document G5OGk02OaxkvNn5V55X7kzVnN

. I I . l.-l.^.yJI , J. ; ,!_.)!. AR226-2951 DU PONT USE ONLY DU Pont HLR 301-90 I : Study Title STATIC ACUTE 96-HOUR LC50 OP TO FATHEAD!MINNOWS, PIHBPHALES PRO Data Requirement United Stated Environmental Pro*e^ 1" J?enCy Environmental Effects Test Guidelines Subdivision EG-9 Author Kevin N. Baer, Ph.D w Study Completed On July 17, 1990 Prforminp Laboratory E. I. du Popt de Nemours and Company, Inc. Haskell Laboratory for Toxicology and JnJu?Jrial Me Elkton Road, Post Office Box 50 '' Laboratory Project ID Haskell Laboratory Report No. 301-90 Page 1 of 12 Company Sanitized. Does not contain TSCA CB5 T:' ."iik ,:$8 /I?'.:-v.lift l3&2i GOOD IliBOHATORT Jmh Du Pont BLR 301-90 rhis study was conducted ae Agency's Good Laboratory PtL ion-compliance are that significantly daofcfuemcetnetdedt*he validity of the study. is* V; Sporrrr: E. I, du Pont de Nemours and Co., Inc. Submitter: Chemicals and Pigments Department Study Director: Kevih N. Baer, Ph,p, Resiearch Toxicologist EnviFomentai Toxicology and Information Services Mo/Day/Tr I i # Company Sanitized. Does not contain TSC CRF I Du Pont BLR 301-90 STUDY! H* 16,267 QUAI TTY ASSURANCE DOCUMENTATION I ernie ACUTE 96--HOUR LC50 PO PATHEAD MINNOWS, PIHEPH/LES PROMELAS AUDITS: Items Audited Protocol, Conduct Records, Report Audit Dates 5/14/90 6/21/90; 7/3,5/90 S ' = S DS pS Tt 7S ,Sto dy d x r e c t o r , 7/5/90 Reported by! ^2* William J. Lynam Quality Assurance Auditor Date -3CWlibny Sanitized. Doe not containTSCACB1 . Du Pont BLR 301-90 iI i 1i TABLE o p o o m s h t s i Page ii ' J2 Good Laboratory Practice Statement ........................ I3 Quality Assurance Documentation ....................... Table of Contents ! ...... . * * 4 ! I. Purpose......... . 5 II. Introduction/Summary . . ........... f Records . . - ............................ 6 General Information . ......... . ................... III. Materials a! Methods j................................. 7 A. Test Material ............ /' * ................... 7 B. Dilution Vater . .............. 7 C. Test Fish ........................................ 7 D. Test Methods . . - ......... * ....................g E. Chemical Analysis . .......... .................... IV. Results and Discussion........ 8 V. Conclusion............... ......................... ^ .g VI. Literature Cited ........... ..................... ............ .9 VI1* TableSl. Chemical Characteristics of Haskell Laboratory Veil ^ Table 2. Vater Chemistry of the Vater Control at Tes^Start . . 10 Table 3. Physical andChemical Characteristics Test Solutions.......... * ` * * * .......... Table 4. Observed Mortality in Percent of Fathead Minnows, Piaeshales pfooelas, Exposed to ,, for 96 Hours in a Static, Onaerated T e s t ......... 4 Company Sanitized. Doss no! contain TSCA CB! Du Pont MR 301-90 I. PURPOSE The objective was to asaess the acute toxicity of minnows, Pimephales proaelas. to fathead II. _______ ',,as not acutelyl toxic to fathead minnows, Pimephales Fomelas, t t|, at. kMir test. Nominal concentrations were 313, 625, of the fish. : RECORDS laborstory^or^oxicolog^and^IndustrialhMedicine^Newark^Delaware^or be Se ^ Sl.cor|.s M!. C.r (iUlngten, Delaware). Technician: &*+ \lfun* R. Scott Vivian Technician;Leader Environmental Toxicology and Information Services Study Director: __in N. Bfer,. Ph.D. Research Toxicologist Environmental Toxicology and information Services Ho/Day/Yr -/S-O Ho/Day/Yr THIS REPORT IS APPROVED FOR ISSUE. 5 Company Sanitized. Does not contain TSCA CBI Du Pont BLR 301-90 Material Tested: Medical Research Number: Haskell Number: Synonyms: Purity: Composition: Sponsor: CE.he%m:isadiusPout d?m'N*emnotusrsDeapn!dtCfon.t, xmnec. Jackson Lab Submitted by: Study Schedule: ttemical^Oiinents Department E. I. du Pprit de Nemours and Co., Xnc. Jackson Lab Study Initiation Date: 5/11/90 Experimental Start ExperimentalJ # e t i o n n5/18/9 Study Completion Pate: 7/17/90 Notebooks: Haskell Laboratory Test Code: Distribution: Company Sanitised. Does not contain TSCA CF! Cj Pont HLR 301-90 III MATRBTAI.S AMD METHODS A, Test Material Source and purity ofm m are described under General Information* The appropriate quantity oc5 S B h |vas measured and dispersed into 4 liters of dilution water (see below)!Inthe test container in the vater bath. Each tank was vigorously stirred p|ior to addition of the test animals. Stability and solubility of indilution water were,not determined. B. Dilution Vater ' Vater (Table 1) originated from the Haskell Laboratory well. C. Test Fish : Fathead minnows tPimephaies promelas) were reared in continuously flowing well water at Haskell Laboratory. Adult fish resided in 38 L aquaria at approximately 25#C and vere fed frozen brine shrimp (Artemla s., San Francisco Bay Brand, Newark, GA) generally twice daily, once on Saturdays and not on Sundays. Eggs were deposited on polyvinyl chloride tiles coated with sand. Tiles with eggs were aejrated and incubated at 25C in a water bath. Larvae were reared in 22 L aquaria at approximately 22C and were fed newly hatched Artemia generally twicje daily on weekdays and daily on weekends. Juveniles were held in 22 L aqkiaria at approximately 21C and were fed newly hatched Artemia and/or frozen urine shrimp generally twice daily on Weekdays, once on Saturdays and not on Sundays. No treatment of the fish for diseases was required during the holding period. Sickness, injury or abnormality were not present. Animals were identified by labels on aquaria and test vessels. D. Test Methods [1] Five test concentrations and a water control were used. Nominal test concentrations were 313, 625, 1250, 2500 and 5000 mg/L. Fathead minnows of approximate age 88 days, mean Length 2.3 cm and mean weight 0.149 g were used. Observations vere made twice dhiiy. Glass aquaria (5 L) containing 4 L of test solution (12-cm depth) were employed. Two replicates of e^ch test concentration with ten fish in each replicate were usfed. Fish were added to test aquaria using random numbers and were not fed for approximately!48 hours prior to the test nor during the test. Mean loading was 0.37 g/L. Tet solutions vere held between 21.9 and 22.7C (mean 22.3BC) and were unaerat^d. A photoperiod of 16 hours light (237 to 269 Lux) versus 8 hours darkness vas employed. 7 CdTflpjjfty Sanitized. Does not contain TSC A CB1 Du Pont HLA 301-90 Dissolved oxygen and pd were w i i i i f o f the test (before the control and test concentrations at the be| EDTA hardness and addition of fish) and daily " trol sample at test conductivity were measured in an analogous water comro f start. | B. Chemical Analysis ; Ho chemical ..lye of the test solution, vere performed. IXV. RESULTS AHD DISCUSSION The dissolved oxygen ^-sinthe * * ^ 2 2 * dropped to below 60* of saturation at 96 hours. T h i p p d oortaiity D.0PPwas not biologically - W " parameters at that test cone'^tration. All e***1 . A1i test solutions (excluding (Tables 2 and 3) were within expected g Al 96_heur lc50 based on controls) exhibited a transparent wbJ^1c ^ ; nt,, tions o f f l W could not observed mortality (Table a n d ofhU S t be calculated because 5000 ag/L failed to kill any oi i V. CONCLUSION concentration tested. VI. literature cited 1. United States Environmental Protection Agency, Office of Toxic Substances, Guidelines for Testing Chemicals, BG-9 8 Company Sanitized. t)oe's hoi coniain TSC CB\ I ! TABLE 1 Du Pont HLR 301-90 Chemical Characterilstics of Basiceli Laboratory Well Water1* Parameter Concentration .I BOD, mg/L COD, mg/L DOC, mg/L TOC1, mg/L Kjeldahl N, mg/L Ammonia N, mg/L Organic N, mg/L Turbidity, NTU Phenolics, mg/L <1C| <1|.0 0.8 <0 . 2 <0,2 <0.2 <11.0 <0.05 Color, Co/Pt <5 Solids total suspended, mg/L <4 Aluminum, mg/L <0.100 Antimony, mg/L <0.060 Arsenic, mg/L <0.010 Beryllium, mg/L <0.001 Boron, mg/L <0.021 Cadmium, mg/L <0.002 Calcium, mg/L 25.4 Chloride3, mg/L 6.3 Chromium, mg/L <0.010 Cobalt, mg/L <0.020 Copper, mg/L <0.010 Cyanide, mg/L <0i.025 Iron3, mg/L 0.131 ParameV r Concentration Fluoride3, mg/L Lead, mg/L Magnesium, mg/L MBS/LAS, mg/L Mercury, mg/L Nickel, mg/L Nitrite3, mg/L Nitrate3, mg/L Phosphate3, mg/L Potassium, mg/L Selenium, mg/L Silver, mg/L Sodium, mg/L Sulfate3, mg/L Sulfide, mg/L Zinc, mg/L Volatile priority pollutants Add extractable priority pollutants Base/neutral priority pollutants Pesticides/PCBs Organophosphate pesticides4, ug/L <0.1 <0.-75 3.9 <0.05 <0.00020 <0.020 <0.1 1.7 <0.1 1.8 <0.005 <0.010 8.1 5.0 0.11 <0.020 ND5 HD6 ND6 ND6 <0.50 Date of analyses 24 October 1989 unless indicated otherwise. 2Date of analyses 5 December 1989. 3Date of analyses 11 December 1989. `Date of analyses 26 September 1989. SNone detected except methylene chloride was below the published Method Detection Limit (BMDL). 6None detected. 9 CTftpany Sanitized. Does hoi contain7SCA CB1 Kfcl : I :;i i i 10 y Company Sanitized. Does noi conlanTSCA CBl TABLE 3 Physical and Chemical Characteristics oil Du Pont HLR 301-90 'Test Solutions Nominal Concentrations Time Vater Control PP 313 PP 625 PP 1250 A1 B> 2500 PP 5000 PP Dissolved oxygen* (mg/L) Start 8.4 8.4 24 HR 7.1 7.2 48 HR 7.1 7.1 72 HR 7.0 7.0 96 HR 7.3 7.4 8.5 8.5| 8.6 8.6 7.1 7.2i 7.3 7.4 7.1 7 .1 : 7.2 7.1 7.0 7.0; 7.0 6.9 7.6 7.61 7.6 7.5 8.6 8.6 7i6 7.6 7.2 7.2 6.1 6.1 6.3 6.2 8.6 8.6 7.6 7.6 6.8 6.7 5.5 5.5 5.9 6.0 8.6 8.6 7.4 7.3 6.8 6.8 5.8 5.8 4.9 4.7 pH Start 7.2 7.2 24 HR 7.3 7.3 48 HR 6.7 6.7 72 HR 7.2 7.2 96 HR 7.5 7.5 7.2 7.2! 7.2 7.2 7.3 7.3: 7.3 7.3 6.8 6.8 6.8 6.8 7.3 7.3: 7.2 7.2 7.5 7 .6 ; 7.6 7.6 7.2 7.2 7.3 7.3 6.9 6.8 7.2 7.2 7.5 7.5 7.2 7.2 7.3 7.3 6.8 6.8 7.2 7.2 7.5 7.5 7.2 7.3 7.3 7.3 6.8 6.8 7.1 7.1 7.5 7.5 1Each replicate contained 10!fish. YSI Model 54A, Yellow Springs Instruments, Yellow Springs, OH. Corning Model 610A, CorningjScientific Instruments, Medfield, HA 11 Company Sanitized. Does not corilamTSDACW Du Pont HUI 301-90 | TABLE 4 Observed o r t ^ U y ^ r e . n t_o_r..b.d Hin.s. MSfgSg B P * * Nominal Concentration (mg/L) Percent Cumulative Observed Mortality 24 Hours: 48 Hours 96 Hours Vater Control 313 625 1250 2500 5000 0 0; 0 0; 0 0; 00 0 0; 0 0: u 00 00 00 00 00 00 00 00 00 0C 00 lEach replicate contained ten fish. 12 Company Sanilftsd. Does no! contain TSC CB5