Document G54xN0aQpNVzjrbqerqZVN4ex
M AS6 - 07-
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE___________________________
Identity: Mixture containing periluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonic acid) (CAS #2795-39-3).
Rem arks: The 3M production lot number was 501. The test sample is FC203CF-X. Current information indicates it is a mixture of 1% PFOS, 20% diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3% sodium octyl sulfate, 1% triethanolamine, 2% sulfonated alkyl ether salts, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect toxicity o f the fluorochemical component o f the test sample.
M E T H O D _____________________________________________________
Method: OECD Guideline 209 GLP: No Y ear Com pleted: 1992 A nalytical m onitoring: Dissolved oxygen concentrations, pH and temperature. S tatistical m ethods: Results were determined by calculation of the % inhibition in respiration rate. T est organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN. T est condition:
Dilution w ater: Millipore Milli-QTM water Synthetic sewage: per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10 mL 1N NaOH, diluted to 30 mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1N H2SO4 and diluted to 1 L with Milli-QTM water. The pH of the reference solution was measured to be 7.6. Test substance was added directly to test vessels, initial pH 6.5. Test vessels: Not given Num ber o f concentrations: one (duplicated) plus 1 reference substance and 2 blank controls Tem perature: 18.6 - 21.6 C Total suspended solids and pH on day o f testing: 2.6 g/L (TSS in final 1.04 g/L) and initial pH 6.9 and final pH 7.9. Elem ent Basis: Respiration inhibition as determined by oxygen consumption.
001276
Remarks: Values corrected to 20 C for calculations. RESULTS Nom inal concentrations: 2 Blank controls, reference substance and 1000 mg/L (duplicated) Elem ent values: 30-minute EC50 = >1000 mg/L
3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS___________________________________________________ The test substance 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L. The 1000 mg/L treatment caused -1 9 % respiration inhibition at 3 hours. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. The study meets all criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number K 19 0 5 -1 ,1992. OTHER_____________________________________________________ Last changed: 6/28/00
001277
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ENVIRONMENTAL LABORAS'TORY ACTIVATED SLUDGE MIXED LIQUOR
SOG
LR Date ijz L tfS L Analyst S A 4 2, P age__L o f_ 2 _
Type Test Q j f . i S
S l o w l y . fos-pircL-itory In h ,b l+ f c n I t J c q
ACTIVATED SLUDGE MIXED LIQUOR
Date Obtained i h t h s L
Date Used k llk h J L
Mixed Liquor Suspended Solids IMLSSI ^ . to
OMLSS in final test solution A V g/L
g/L Adjusted to
g/L
Initial pH A . e\ (1st test soln. prepared)
Final pH 7 -1 f (last soln. prepared)
Comments ________________________________________________________________ _____
TEST SUBSTAIiCE
Sample Description FC - ' 2 n ' S F~ X________
Initial pH of highest cone, tested ( / C C C ^ / j - L . T
Comments __________________ ;______________________
Adjusted to pH
REFERENCE SUBSTANCE
Sample Description
k U r \a o 1
Initial pH of stock solution ( J ? D V > mg/L) V 'x' iVi"* -vc,.
Comments
/
EXPOSURE CONDITIONS T f f
Test Duration
a' ' -S'W
"V s*>
Adjusted to pH , '/) ? , w
V '*' `S,
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iR t T M o s ^ - l
ENVIRONMENTAL LABORATORY
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OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
TEST SOLUTIONS PREPARATION
beSTUpy available
ENVIRONMENTAL LABORATORY
LB K I t o S - I
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance: F C - 2 & 3 C F -X
Source of Activated Sludge Mixed Liquor: M e \r o LQ aiA & Date Received: h J z b h K . , Analyst: j k r t - d - A ? \ jJ \ -
Date Used:
M AI
30-MINUTE EXPOSURE
Test Substance Concentration (ng JL 0.5
1.0
DISSOLVED OXYGEN CONCENTRATIONS, mgil.
TIME-MINUTES 1.5 2.0 2.5 3.0 3.5 4 .0 4 .5 5 .0
Temp
5 .5 QC
BK CONTROL I
SjJP) 4Qi'5 &
7-9 Z A 7.4 L A Z L Q .
kJe. fa A ( i A
s a 30i/
j o Q ^ piiAk ^ > b p ll crt
2d S O . 2 a Q_ H i L k L A
3
23 z Z K
2S 2 2
a s : AL 2 Iz
ili . 2 2
BK CONTROL H
7.0 L l i .S A Z . J fL S A A L L S I 5 .X 5 . 1 H -2 2 ^ -7
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONC0URATIONS, mgIL
:tmi
Test Substance Concentration (
) 0.5
i f BKCONTROL ! k:-.
I o fe-,
s .d UT
s'j N i . p / i /Ci4 p 1 .................. . ? V"r,
i.-.--S.' '-T': y v
1 1.5
t t M U
U R > SS*
TiSr/i
2.0
Ui (o. (o
TIME * MINUTES . 2.5 3.0 3.5
is
S fR
SU
( e r f 7 ^ a Cp . I -m *.-
/ r.
* Temp
4.0 4.5 5.0 5.5 ; c v~
V.-j,'-K`V V- V> S,,v
l a * ^> 4 S 3
' K'
1 5 .6 ? /
*5 .0 a i/o ' :v;$Vy.'
S R \ i# . S a o t m -*- i } -
mm
l$
-*WWS-5SW*t.
m M - ^saaaa
B W i M a 7 t H m il f? 7
LA w &
s m
WM
pH of activated sludbe mixed Ilgudr^S Initial - y . ^ ; "v-kz
,pH of highest concentration |T e s t|te m p e ra tu re
hX f-tV-jK?*
* - .1*'A
001280
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
Test Substance: FC-2Q3CF-X
Analyst: SAB/RJW
- Lr:-; K1905-1 f.UDate-j6 /2 6 /9 2 l
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ma/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp,*C
Ra (2) ' ^ In te O )?
mm
m m m l iw i
BK1 ' 7.8 7.6 7.4 7.2 7.0 6.8 6.6 6.4 6.2 6.0 5.7
20.1
0.4109 0.4081
w
1000.0 3.4 3.0 2.7 2 .3 . 2.0 1.6 1.2 0.9 0.5 1000.0 3.7 3.3 2.9 2.5 2.2 1.8 1.5 1.1 0.7
21.6 21.3
0.7190 0.6433 0.7286 0.6656
0 ->
o
.f
... .... ,, v ", a.
BK2 7.0 6.7 6.5 6.3 6.1 5.9 5.7 5.4 5.2 5.1 4.8 20.7 0.4206 0.4006
% diff in controls (4): 1.8
EC50 (95% Cl): > 1000 mo/L
RaCm (5): 0.4043 .. V~-` - <
-' ' j- - i
mo/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp.'C R (D Ra(2) % Inh. (3) -
m m It **- ^ i "
-
- *< iiiiiii
BK1 8.4 8.2 8.1 7.9 7.6 7.5 7.4 7.1 7.0 6.9 6.7
18.6
0.3394 0.3741 /'V
,
1000.0 6.7 6.4 6.2 6.1 5.9 5.8 5.6 5.4 5.3 5.1 5.0 21.0 0.3152 0.2940 -- 23
w a "I-
'
1000.0 7.1 6.9 6.8 6.6 6.4 6.2 6.1 5.9 5.8 5.6 5.3
BK2 7.3 7.1 6.9 6.7 6.6 6.4 6.2 6.0 5.8 5.6 5.4
% diff in controls (4): 2.9
EC 50 (95% Cl): > 1000 ma/L
20.8 0.3442 0.3256 14
,;;k r&C*
19.6 0.3745 0.3851
s<?ti r 7 RaCm (5):.H0.3796;i
(1) R> Oxygen uptake rala (mg 02fl.-mln) (2) Ra. R a d M *d to 20*C> (Rfr.Ex) (3) % InhUHona )(RaCm>-R*S/(RaCm)]l X (4) % dHatanea In control*: (lRaBk1-RaBk2rRaCm]100 (Two control* must be within 15% ot each other.) (5) Mean *d)ust#d R. controls- (RaBkl RaBk2y2
1.072
RaCma mean adjusted R, controls ,
; ;?, .( v .'i
Bka blank control, Sa sampia
Tobsa sample tamp
V
x* Tobs-20*C
^Y.~%
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ENVIRNOMENTAL LABORATORY
LH k l l j T
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #20 9 TEST SOLUTIONS PREPARATION
Tost Substance: 3 y y>~ T > \C k
1___ vc?
Sam ple Description: / 4 - U f i r J t Q<?A IcvJxJL L c r \r
O O - Q ________
Analyst: < L
Date b f a b j 43 .
Stock Solution
P reparation: 5 Q C > Hys, I t j,
< x c l ^ ___ i p f t f J L - O S m
\y-ed S o
tri io tv JL
L O N A/tg<^
L>ifufec( A ic X
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iim -HT m .iti-c : a.^4eT H -a Co/ r i c o p u .rise L -n + il itTC! p i e n i - p>pH-
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S ^ ,fk Sy^ltn p h i ~ ^=>
Test Cone.
mL added
mo/L__ per.SgQ-mL
B * C n frol
--
mL MQ(1) water mL syn. sewage p9r.Sgft.ml... iaodjar-SflO-mt.
A A 4___________ L k _______
JjCL
l Q -,
2 z
1L.
mL microbial
iaaculunuwr 5.00 mL
Total Volume
...mL.
Soc>
J j2&.
i _j
. ' j*" .
001282
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ENVIRONMENTAL LABORATORY
LR K 19 0 5 -
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance:
T 2 > l d ^ ) o r o p W n < 3 \ R e > ( t > n r f ~ > o i ________
Source of Activated Sludge Mixed Liquor: JAe-lm U/>$4e. J {g arm e n t-IF ]***- S r jiJ^ M /0
Date Receive#:
Date Used:
Anaiyst:
iX Q C c k rP {
30-MINUTE EXPOSURE
Test Substance Concentration ( f ^ J ) 0 .5
1.0
DISSOLVED OXYGEN CONCENTRATIONS, mg*.
TIME-MINUTES 1.5 2.0 2.5 3.0 3.5 4 .0 4.5 5 .0
Temp 5.5 C
BKCONTROL 1 ID
Ztf 7 l > 7-4 7 . * 7 . 0 . s . f > . 4 ( r . . O 5 - 7 J c - \
1,5
1 ,3 i . a 1-0
<#/3 ( p .6
G.-S a a s_
BKCONTROLO
T O G.1 6 .5
G?-( 5 .1 5 .1 5 .4 5 . 5 5.1
3ai
3-HOUR EXPOSURE
m r:
DISSOLVED OXYfGEEN CONCENTRATIONS,
........l<........ -.'r.R..:.'.*,.,.........
mg*.
v. fVjSt--s:>-'
'<
Test Substance
\*&<iConcentration
..... .,fc T IM E - M iN irTES
* a, ' 7`
.ty. . Temp
) 0.5
1.0
1.5
-W
.
2.0 2 .5 3.0 3.5
'-'V'S.
-..,4
4:0
4.5
- ..-..1
5c *0 ^
5.5 V'
:-1 M<C . '
BK CONTROL 1
i tf'V;
S I 7 . 4 7 .( 4
. .'-i 7 7 .1 l O
w-
?/& &
'Id #**&*.1 -: r
" i . i t i t 5 ?,0 W
1 . 1 1 -G* 'S-iV
v $ li/
JO iV
mm t
f4 i>v . ' . . ,
ssteof
H K m m . W
v-V ' S'**
&BS&
:W -.^
WPH'
-.7' m m
-
flPMt:*-' .
m m '--jgV
:M0 S M i
saSfc
I M 1 B P W
jJSgpfcS&.'i&
<-.'t m m -
%pm pPPSife.:
I bKCONTROLO
' & $ 0 :
f f i t j lip W M B K
m5 . (mP m
I
pHof activated sludge1fnlxed .liquor: ^ In itia l,_______
fpH of highest concentration tested:
initial -**7# O
Testitemperaturety y ^ ^ ^ "
.8
I K-bt
001283
[ENVIRONMENTAL LABORATORY " S
v m ffifx c D .Ac t iv a t e d s l u d g e r e s p ir a t io n in h ib itio n t e s t #209
flC ^ U U V n b N O F ,R E S U L T S ;^ V :
3.5-DiCHLOROPHENOL
ryy--
Lr: K1905 Date: 6 /2 6 /9 2
% 3^3
BEST COPY AVAILABLE
't Ima/L^l ^ 0 .5 * 1 i 1.0 1 1.5 I 2.0 I 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp, *C R (1) Ra (2) % Inh. (3)
- ,, L i . l '-y' . :
wWsmmsmm
Ittlif
,,V ' " H l i i l
BK1 s?7i8l * 7 .6 7 .4 7.23 7.0 6.8 6.6 6.4 6.2 6.0 5.7
20.1
0.4109 0.4081
>* .J
# 10 r 7 .8 ' 7.5 ' 7.5 -7.3 7.2 7.0 6.9 6.8 6.6 6.5 6.3
$$$$$
;`$W
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fei# yy^y `i*, A.
ep*** `><Sr$ev 4T'*>' i ; . . , '.
isvSFs^e' TiWTffliwirTMtiiff!r1'
'i K ~'
20.8
0.2739 0.2591
36
B K 2 ^ 7;0* * 6 ; 7 6.5 6.3 I ' 6.1 5.9 5.7 5.4 5.2 5.1 4.8
20.7 0.4206 0.4006
1 . 8 ^ ^ ^ , : ; EC50 (95% Cl):
RaCm (5): 0.4043
3-Hour ExDosure v ^ ^ j S
1
Dissolved Oxvaen Concentrations, ma/l
1 ma/L H 0 . 5 'l M . 0 I 1.5 I 2.0 I 2.5 3.0 3.5 4.0 I 4.5 5.0 5.5
1
1';" L ... . i V. . ^ . J
i. i aaAl/afr./.vi i
' i V V `r-r > W
$ BK1 * 8 i 4 * $ 8 .2 8.1 i 7.9 I 7.6 7.5 7.4 7.1 I 7.0 6.9 6.7
i
1 *1 0
S8:Tife
v,, 00 O
8.0 7.9 7.8 7.7 7.6 7.6 7.5 7.4 7.3
if ^fcfc:x
, ....
1 w tm # f mmm
l
1 BK2 * 7 .3 I 7.1 I 6.9 6.7 6.6 6.4 6.2 6.0 5.8 5.6 5.4
ditymwntrols (4):^.. 2.9 ^
EC SO (95% Cl):
Temp, "C I R (1 ) , R a(2) [% Inh. P ) I 18.6 0.3394 0.3741
20.7 0.1600 0.1524
60
19.6
0.3745 0.3851 RaCm (5): 0.3796
lOJSJKO-xygen urpta-ke n ie (mg OZ/L-mln;
sHJrfRaa.R.
to'2Q *ci'-' [R/s.Exl
KRaCm)-flaS/(RaCm))100 3
rtM w w ^ w M rtM ^ lia tfk l^a S U V R a C rn J IO O (Two oonlrols must bo within 15% of oach other.)
eft- . . .* - % ^
_ /n. , . . ,
^ U o iM (ftaBkWRftBU)?
**" i*!tr' ,,_
wVv.
,,
.vir-ri^k?J 5Aik^7^9^W#!4l^"->*rv
:y ."-. p '^ y .-,
1.072 RaCm> moon adjusted R, controls Bk blank control, S> sample T obt, sample temp x . Tobt-20*C
mmm
0 0 /2 2 5 "
Additional Comments': ' " '
'. 5.
'*. *.
,'A .Jt. rf; V
`
;' - ,r" i. '*.
,r ^ r1'. ,' "' t'S'..-. ;*
SEE REVERSE SIDE FOR INSTRUCTIONS
001285
