Document G0NJwV4NnVLQxXoM5N4jKO6Y
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AR223-0079
El-17
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LaboratoryReport
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Analysis of Fluorochemicals in Wild Bird Livers
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Laboratory Report No. FACT-GEN-010
LR U2392
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TestingLaboratory
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3MEnviro3nMmeEnntvailrToenmcehnntoallogLayb&orSaatfoertyy Services
Fluorine Analytical Chemistry Team (FACT)
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2-3E-09 935 Bush Avenue,
1
St. Paul, MN 55106
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Laboratory Contact
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KriBsldHga.ns2e-n3,B-P0h9D.
St PauPl.,0M.BNox535313331-3331
`Phone: (651) 778-6018 FAX: (651) 778-6176.
Requester
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foundan aboutthecauseofdeath iknow) reincludedinAppendix Coftisreport.
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L`iwvereerqsuaanmtpilteatsiwveerleyanhaolmyozgeednfiozrePaFnOdSeaxtnrdacPtFeOduSsAinusgianngihoing-hpaprriensgsuerxetrlacitidonprocedure,Theextracts
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cboyntFaAmCiTnainnstahoed3mMeEtanbovliitreos n(mPFeOnSLtAaAl.,TMh55e6pr,eMsSenTcOe,aPOaAbsAe,ncMeSo1f3o,thPeFrkHnS,owandfuPoFrDoSc)hweaiscdaeltermined
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bsyitnspeacitnicoAnpophfenHedPiLxdCB/.ESMISMSanalysisof 2birdliveswhaechgroup. Aful stofaconyrmis:
`Withthexception ofmotof theSavillCranesan asingleBrowaPelicanfomCalifia,thecats
o Tmt pn ne a Sir (exMtrDacLts=c1o0nptapin.edEsPtFrOacStlsevfreolmsvtehreylnievaerrstohfethMrDeLo.f 15SandhillCanesshowedsomePFOS;twoof these
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sEhxtorwacetdsPfRroOmSolneeeBsriangcr'eaCtoexmoocsraonffthoosm dSeatneDrimeignoe,dCnAtahedeoxtnraecWtshfitoemPoetlhiecranb,ifrrdsomwCialniatthieas,aCmeA:
classification(20xforthecommorant an eary10%fo thepelican).Ifthesetwocutlpyoiisnagre
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t`hreemgoevogerdaapntdicPaFlOlSoclaetvieolnsowfitthheibniarpdarhtaibciutlaatrclcaasnsbifeimcaatdioen.are averaged,somegeneralobservationsbasedon
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`DWiietghot,hCAc,xsCpltiioaniaof,thCeASensdhhoiwl rCealanteisv,eliynlgoewnleervaell,siovferPfFrOoSm,bwiidtshaconlalveectreadgferofm ethewheasnte5r0 npUbS.(LSiavners
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from birdscollectedontheEastCoastoftheUS(Mia,Naples,andPL.Ladera,FL)showmoderate
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P`wFeOreSdleetveerlsm,inaedpfporcoonxtaiinmt1ah0teh5eipglphbye.stElxetvrelasctoffPrRomOSb,iradppvreorxsizcaotlelleycte3d0f0rpopmbt.heCentalUS (LAandNV)
mIotrseuntlhiakneolyntehintstthaencdei,fftehrenscmeseasspseocciiaetseofwbiitdhPliFvOinSglienvaeldsifftrheeatevnevrirsoanmpeunrteslhyaowfeudcsigoanioifcsaptelcyies. Tn
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cdoifnftearienntaPn aRvOeSrlaegveePlsF,OFSocroenxcaemntprlaet,iovneorfs5c8llpepbc,twdhfirloemvehrist coplelleicctaendsfirnomCaWlihfiotrneiPaewleicrandseien rNeivoaedda1
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sihvoewredraovmetrhaegseebPiFrOdSswleerveeldseotfeorvmeirn3ed50opbpe.wnSiafonrhallyllCoawniensPaReOSan,erxecgeaprtdiloenssofttihsegcehosgerravpahtoinc,laoctahteon.
ofthebinds.
u fies aes teaia cachcaswieredietercminead 0ciontaoinPFnDS.Live extractsfom severalspecies,inching 1
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d`eSatnedaumiillnCerdatnoec,ownetraeidnetselrmlinaemdotuonctoontfaPinEOasSmAa.lNlaomoPuOnAtAo,fMPFSHIS3.,NMeSaSr6l,yM2S0T%)o,fotr ePlRiOveSrAexAtwraacst.swere:
detectedinthe iverextracts romamyofthebids.AppAceontnaintdheseiresuxlts.
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tissuesamples.
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pHrioghdeusctcsuuiltatbyilidtay orvseya.lReasutlitsstimoeu.ld beconsideredto besemi quaniaive. Da presbeerenhte.
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LMOaendbi,c1si5an/me9p),blJeyeawSnceBorteutrP,osHafcrndosmef3nrMofeOtnch.ecuTNpahtaeitsoianomanlpallWMeesidwlieceirnHoeradgelilnitavleClryeedsretnhiettnboMiGneaadrvyeoesO,alWmsipeslnc(e3osMstioOnt.hseEentvirroan]mcaal
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"Therei oholding imecriteriaasociatedwiththesesamplesforLCESMSMSanalysis.
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Anl"yFtAiCcTaMl-m1e0t,hEoxdtsractionofPotassiumPecooctancefonteorOtherAvior Foceosbemical
SuFrAfaCcTt:aMnt:s2f0r,oAmLsiivseirsfoofrAFnuarlyoscihseUmsiicnagsHiPnLLiOvEerSEMxSact Using HPLCEloctosprayMassSpectrom
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SaAmmpalyetperiepcarxaaticoenf- oaqmuaeosuasmspalmepmlaest,iHPwiLtCh/oEpSiMSnMgS:reiaogne-nptarsintgoexttraacmtimoon hydrogenslit
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(OTnBcAeYthienaanpotnTcoBnArolaliedienfvoirromnem,enht.eTahlectatisotnaincrseeargeendtvetloecti5valyotalrrgetsgaanoinsefanotroc(hiepmlicals.
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acta)dried,adrconstittdinmethanolforMS lysis
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BPILcnoCl/EumEPnSL.,MBaaSssaeladidoqnuHotthoefPacffrinaLitcyoifOstihnejEaencftaoelrdSydatenetdaiMpolraetsdhsSqeeusdatltiMotouatngiShyvepawrhoe:avrsekersnepehasceliquidcehlratoimvaettoogtrhaepihiicd
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s`omloubtiilonphPaFsOe,Stmeaaynahlycaits re0imniemefsor.aRcehtaernatciioenistimceasmboeutnwteeonf iest.andFaorrdcPaROmSes,oliuntaiosntaannddahred
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analyteextracedfomfiver this analysiswerematchedontheHPLCsytem 0within 1%
FollowingHPLCscparaion,ESMSMSprovides aapdndaratemeans foranalyzingaviderangeof
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a`donertdgeaacntpiicrocinmoafromylonme, chpianracoclontuesdr,iiu nssgtgifcen lnouefortrad holeclhayenosmapilecyrat,laes,t.iesdEasetllleecitcvedet.Tlrhyiomsasndoiponntrisezapabetyorimos,bntartredsee.dMcwoihlhuhsneiedgsihpreaqinrmeeaurcrgiylezgy af,sor,
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dbesoeednt colons retesalle secondary onicagentswetthepinyin,which are
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msiegcohntdhaarvyeothnefsaagrreescthaatriaocnteraitsees49.9 csprimeyin,but fenchemical compositionsand
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5.1 Calibration
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`asnotused forPFOSdetermination;bracketingcalibrationcheckswereallcompliant, 5 thisexclusion
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`doesnotadverselyaffectthedataquality.
52 Blanks
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{`hBilsanstkusdayr,ecaolmlplnikantwiefrneotcaormgpetiannta.lyteisdetectedabovethe limitofdetectionforaspecificanalyte. Tn
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53 Surrogates
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Teahyoporocane fc iswd a5 8supinthis rls. Sugerespons:is
`monitoredto confirm grossinstrumentalfailure,ifnecessary.
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54 Matrix Spikes
`Duplicatematrixspikenyse wing olltargetanlyteswerepreparedandanalyzed romSacil
`craneliver.RecoveriesofPFOSandPFOSAwerewithintheacceptable rangeof70-130%.
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55 Laboratory Control Samples
LaboratoryConrolSamplesar notacomponentoftis dy,
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5.6 Sample Related Comments
`Matrixspikeswerepreparedfor asinglespecieofbird. It iassumedthatspikerecoveworuildebse:
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r`eassoniafbomlreairleltlsenpteaicoinerst.iNmeoasntdarnedaasrodnmaabtleeridaalufgohrtePrFfDoSnisspaveaicluab.lUe.sCtolms pouna dimdenatn itfiicaitd isoanviislr baabsleed on
identification of thisanalyteshouldbe considered tobepreliminary.
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pe pdra OSde emt t `Withtheexception ofmostoftheSandhillCranesand asingleBrownPelicanfromCalifornia,theextracts
from theliversofallofthebirdsweredeterminedtocontainPFOSabovethemethodlimitofquantitation
n A `extractscontainedPFOSlevelsvery nearthe MDL. `ExtractsfromoneBrandt'sCormorantfromSanDiego, CA andoneWhitePelican,fromCalipatria,CA
on
trh` eemgocevoegldraaa pnhdi(s cP>aF2ls Ol0oxSclfi aeotvrief otlnhosewi fcitothc rehmibona irarapdnastrt'tahini acdublnio aeraarcn cllaaynssb1ie0ixmcfaeordriteohn.earpelaivcearna)g.eId,ftshoemssetgewnoeoraul otbslecpvyoaitinitosnnasbgraesed on
WDiietghot,hCeAex,cCeaptlipoantoifat,hCeAS,asndhhoiwllrCerlaanteivse,liynlgoewnleeravle,llsiovferPfRrOoSm,biirtdshcaonlalevcetreadgferaofmtehssewtheasnt5er0npUpSb,(SLaivners
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frombirdscollectedontheEastCoastoftheUS(Miami,Naples,and Pt. Lauderdale,FL)showmoderate
un is lilE y tmeeilestI h p vi ROSlo olS s rhesvmeS seb asoplsyeectSEionhoefesyi. In `Weredetermioedtocontainthehighestlevelsof PFOS,approximately300ppb.
`contain anaveragePFOSconcentration of58ppb,while liverscollected from WhitePelicansin Nevada
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`showedaveragePFOSlevelsofover 350ppb. San Crand esah reai n exl ceptl iontothis observation,asthe:
Ta nkware delon 5swhirl lowinPFOS,regardlessofthegeographic location
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`Withthe exceptionof th SandhillCranes, ive extractsfrom lastaneofthetwobirdsscreenedwithin
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`eachclassificationweredeterminedtocontainPFDS.Liverextractsfromseveralspecies, including 1
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es `SandhillCrane,weredeterminedtocontain asmallamountofPFHS.Nearly20% oftheLiverextractswere
determinedtocontain a smallamountofPFOSA.NoPOAA,M513,M556,M570, or PFOSAAwas
|
000524
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BOr OBrLboRr ATORY
Thisdataindicatesthatpotentialgeographictrendsassociatedwiththeenvironmental distribution ofPFOS derMocrosteicalsmaybe Gadble by characterizationof ow eve of hee composi anal
`Thisreportreflects themost accuratedataavailablea this time.Allmumbersshould be consideredfo besemi-
`Thisdataindicatesthatpotentialgeographictrendsassociatedwith heenvironmental istibuation of PFOS
`maybetraceablebycharacterizationoflow-levelfluorochemicalsinanimal tissuesamples.
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`Samplesandextractswillberetainedforfisture evaluation,ifnecessary.
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: 1007 '99 08:43 ID:A@naETlEySsi4s2-2o8f Wild Bird LiFvAeXr:s6.12-778-4468
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#=notdetected NA =notanalyzed x = detected LOQ=Limi ofQuantiation
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L JV---- Fluorine Analytical Chemistry Team
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PFS's
nN
PFHS `perfluorohexanesulfonate
C6F13503-
PFOSA PFOS perfluorooctanesulfonate
nN PFOSAA
CC88FF1I775S0032-NH2
C8F17S02N((CH2CH3)(CH2C00))-
MM557506
CC88FF1177SS0O22NN((((HC)H(3C)H(2CCH020C)0)0-))-
BM
rosea pertuorcorsmenifonyietyt Et-FOSE-OH
snide
C8F17S02N((CH2CH3)(H) C8F17S02N((CH2CH3)(CH2CH20H))
BW PDS perfuorodecmmeitonate
CIOF21503-++
PECA's WM ohn retoenons
MSI3 perforodecancate By VB petuordodecaone
MM46633 HB we
C7F15000COF19C00ClIF23C00CCI80FF1271CCO000-C6F13C00-
--
BW ors petuonimet mito PFCA = perfluorinated carboxylic acid
n
10 standard material availableforconfirmation;
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000529