Document Exr4XN4revqjDYQRQnEMLzZn0

LIA12310 * 4k i I - i ?l - J 1 i j itiftBi ~Titfiaiiiiwraitfinri7ii*T`f'f<*f'"' t t iii........i ;' LH-f3i. A PROPOSAL 10 International Lead Zinc Research Organisation, Inc. rot 1. Effect of lead oa tissue culture of the nrvous system. XV. CcKp*ratlv study of the pattern of absorption of lead, tin, mercury and copper by nervous tissue in culture. 2. The influence of lead and other heavy Metals on the synthesis and turn-over of RKA in mammalian cells. Proa July 1, 1967 through June 30, 1968 Total Support Requested: $13,000 Hanna Scbkowics, H.D., Principal Investigator Department of Neurology Medical School The University of Wisconsin 730 University Avenue Madison, Wisconsin 33713 (Signature) Hanna Sobkovlcs, M.O. Principal Investigator (Signature) franc is M. forater, M.D. Department Chairman (Signature) ' Peter L. Elchman, H.D. Dean, Medical School (Signature) Len Van Es, Director of Research Adalnlstratlon-finane is) PMWJ* mm 1 ' 9\ m LIA12311 jjflfgatHte : MUM International Lead Sine Research Organisation, Inc. 292 Kadieon Ave. Mew Yorfc,M.Y* 10017 APPLICATIOB rOR RESEARCH CRAJTT Date Rb. 37,1967 Application is hereby made for grant in the a*ount of S 15.000 for the period free July 1. 1969 __ through July 1.1960 inclusive for the purpose of con ducting research oni 1.- Effect of lead on tissue culture of the nervous yst--. rv.- Comparative study of the pattern of absor ption of lead, tin, nercurv and copper by nervous tisaue In yy^rti 7.- The influence of lead and other heavy natals on the syn thesis and turn-over of RKA In seeretlsn cells.. Mane and tills of principal investigatori Hanna H. Sobfcoulcs.H.P. Assistant Professor of HrurMocy. Address ,including nane of institution,of principal investiga tor t Tissue Culture Laboratory of the pr>artnt of neurology University of Wisconsin Kd>cal School.Kadison.M)sconsln ??7Q6, Raise and title of financial officer to vhoai checks should be elledt Mr. L. Vsn-gss,, Director of Psrch A^inlstrstloar- flnsnclal. Address, including naate of institution, of financial officer! University of Wisconsin, 750 L*r>lvermy Avt.^Jla41lOfl,wiii.5J76ta- 4 i Mg--Miseaanrnweiuti-naMMffcsieiaaheaiwlrtiaei See-nae i k si!**lipjpsi^^ LIA12312 mem, jS2b&aimmskiaii&tm aia>i " .-jj,,> jn .* ** --;. -a- SUDCET rOK THE MJHIOD fROH i JULY, 1, 1*67 - JULY 1,1968 Permanent e<ru 1 pn>nt AM8C0 Dualawtic Deioniser ..................................... AMSCO puroeeter and Conductivity Mtr with 24-h graph recording ............................... Mlcroaeopic equipment ( abort diatance lanaaa and condanaor) ............................... 6800.oo 1700.oo HOO.oo Consumable auppllea Glaaeware .......................................... ............... .............. Small instruments & mine.Lab. supplies................ Photo supply a vorh ....................................................... . Chemicals 6* precursor#.of nucleic acid*................... Aniaala .................. .............................................................. 1000.oo SOO.oo 500.oo SOO.oo 1000.oo Otbar expense Xerox for bibliography uae........................................... 100.oo looka concerning toxicology & poiaoninga........... SOO.oo Heavy metals teata ....................................................... SOO.oo Travel expenaea................ ............................................ ttarroTAi, SOO.oo isooo.00 1-IA12313 --* natm mm -3- "" JUSTIfICATIOJI or BUDGET ``r* - ~ Tit* hiatocheaieal port of our project require* axtranaly pura water.. Jteagenta uaed to datact haavy natala in a tiaaua aample ara not apaelfie for a given natal, tharafora any contamination of water with other natal* in tha watar aupply nuat b* avoided. The water in Nadiaon craataa a apacial problem for our raaaarch progran aa ita hardnaaa ia ranging fron IB - 22 grain* ( i.a. high calelun and magnesium)i apacial arrangananta ara raguirad in addition to alraady exiatlng apparatuai AKSCO ultra Pura Watar Still plua atoraga tank and Anarail Quart: Bl-Diatillation Apparatua. To pro* vlda ua with tha purity of vatar naadad, our anglnaaring dapartnant haa apacifladi 1.* AKSCO Dualnatlc Dalonltar which automatically providaa a eon* tlnuoua aupply of daionitad water of 1 negohn-cn apacific raaiatanca. 2.- A apacial final control lncludae Puronatar which automatically divert* diatillat* of laaa than daalrad purity ( 0.1 - 1 magohm - cm) to vaata line. A conductivity Mater with 24 hour graph recording of tha diatillata delivered. The** 1 ten* of equipment are aaaantial if va ara to be aasured of tha .chamical apaclficlty of our method*. HPWyi.-!iU *P mw --Mi LIAl2314 liBtTr-1 -''^Ullii^li8ffiiiiilliilfrTnTai*^^ rwii-i*afc-Mfc.--wi ai*r.w Uidkij -4- HZSSAftCH PLAN ` Principle lavtitijitori Hanna ft. SobVowici.M.D, i Aaalatant Profaaaor of Neurology# Tlaaue Culture Laboratory of tho Department of Neurology, Univerelty of Wisconsin Ntdicil School Project aaalstenti Rachel Monrain,B.A.,R.N. Project Aaaiatant of tha Tiaaoo Culture Laboratory of tha Department of Neuro logy, University of Wisconsin Medical School. 1. Iffacta of lead on ttaaua cultura of tha narvoua avataw. IV.Ccxrrtrat 1 v atudy of the natt-m of ahiorrtlon of lad. tin, mercury and cooper by narvoua tlaaue In cultura. Tha princlpla ala of our raaaarch project la to Inveetigete hlatochaaically tha cella which abaorb tin, aercury and copper aa compered to lead in narvoua tiaaue in cultura. It vaa demonstrated In our prevloua study that heavy Mtala can be divided,depending upon tha reeponae of cultured narvoua tlaaue.into two groupti 1.- Hetala which appear to aaart a direct toxic action on tha nerve call { aercury) and 3.* Hetala which are not toxic for tha nerve call itaelf but deatroy tha upportlng tiaaue ( lead, tin and gold), furthermore on tha j baala of a hietochemicel atudy. wa demonstrated tha praaanca of lead In thoaa eupporting tlaaua call* which suffered Injury under axpoaura to tha natal.Tha hypotheaia waa m4i that laad *3* k V "-WW m LI A12315 ---'- ..... -- ^Htif'Kr'inririliTiifl'J- -^v,, > . awifeiai - -- -5itself has no direct effect on the nerve cell or the myelin sheath in vitro, this nay be doe to the demonstrated lack of uptake by the nerve cell from their environment. It is also suggested that the excessive absorption of lead by mesenchymal tissue results in the degeneration of this tissue. In order to prove our theory that the toxicity of a heavy SMtal is related to its ability to penetrate Inside of the cell.ve propose to cos^are the morphology of nervous system cultures exposed to a series of SMtals with the hlstochemlcal determination of the metals' absorp tion by nervous tissue in vitro. Tin, mercury and copper have been chosen for our aeries.Copper is included because of the work of UxMn ( 1956)*. He found that copper, which is one of the most toxic metals tor nervous tissue, is absor bed by myelin sheath and nerve cell nuclei when sections of normal brain are exposed to the Mtal. The hlstochemlcal study will be per formed on absorption of two known toxic metal it mercury and copper, and two metals which did not show a direct action against the nerve 1 cellt lead and tin, * L.Lahut Usman i Hlstochemlcal Localisation of Copper with Rubeanic held. Lab.Invest.,1954,Si299-30). 2.- The joint project with Or.Henry kubinsky.Assistant Professor of Surgery,01vlalon of neurosurgery,Universlty of Visconsin,Medical School. ThS-lntlmac* at Usd. and O'.her ltsvY fr-tals on the Synthesis and Tyrn-oysr of WA in Hawaiian Celia. IntroductioniThara ara several observatt^n* on binding of haavy metals to dsoxyrIbo- and ribonucloic acids ( >A and wi) and tha possible biological significance of such altered n*cromolecu)#a. To the best of our hnowlsdge, however, no thorough w>rk has been done on the Influ ence of heavy metals on the aynthaaii of RKA in the treated cell.One i." -. ..ijuiywiiiip^^ LIA12316 mp p ,vW^T*^4hu,.naaaadm-e. , can expect that tha pattarna of RJU aynthaala ( l.a., tha ralatlva rataa of aynthaala of rlboaoaal precursors and `messenger* RNA'a) aa wall aa tha over-all rata of callular RJU aynthaala May ba aigniflcantly changed In tha call undergoing auch treatments all traataanta which might ba conaldarad * .toxic* or *Straaaful* to tha call aaaa to raault in profound altaratlon of MU Mtabollaia ( raf. 6, 11. 16). Experimental techniques! Tha aynthaala of MU will ba atudlad with Hawaiian call* la tissue cultures and in living organiaa. For initial atudlaa, howavar. aodal * S 180* mouse ascites tuaor calla in suspension will ba uaad. Tha infonaationa obtained with tha aodal ayataa of * S 180* aacitat call* will ba aubsequantly uaad for further atudlaa on tha aataboliaa of narve call* la vivo and in tlaaue cultura. Tha aynthaala of MU in thaaa call* will ba atudlad by axpoaing thaa to tha atari la aolutlona of aalta of heavy Mtala ( or a combination of auch aalta) followed by a pulae of radioactive pracuraoara of MU ( FJJ> KJ or C14 laballad uracil or uridine). In control experiment* tha call* will ba treated similarly in i*dla fra* of heavy metal*. MU will ba extracted by routine methods, fractionated and analysed byi chromatography on aethyl*esterfl*d albumin-Viaselguhr (KAK) columns) by sedimentation in sucrose gradi*nt*i by hybridisation with WU and by th* analysis of nuclaOtlde composition of various MU fractions. The** technics will allow separation of various hinds of MU * ( transfer, messenger and riboeosaal ) and permit recognition of the petterna In MU metabollaa. HS"> i ?-W1 J'i H, H P | l l P W W " 1 11,1.... * "'i** mu* i it lriio . h>4i.......... .An. LIA12317 / htfm I Ififr rfr'ifr 7- 'i Zn addition, tha faat 1abalad RMA darivad from sub-cellular particlaa ( call nuclei and Mitochondria ) will ba atudiad in parallel. Tha iaolation of thaaa organelles will ba laada by routine methods. i under thaaa experimen tal conditiona tha pattsrns of MA aynthaaia ( amounts of radioactiva precursosrs incorporatad into various MtA fractional will furnish tha information on tha regu ) latory mechanism. Tha techniquas daacribad will alao parmit detec tion of any MCA normally abaant in untreated calls, but appea ring as a result of tha treatment with heavy metals. An aasasmant of tha usefullness of tha acridine - orange staining technique for tha above atudiaa will alao ba made. Significance of tha proposed research! It may ba expected that informatlona gained from these studies will Increase our understanding of molecular events resulting in call injury and death due to heavy metals intoxication. It will also increase our understanding of tha regulatory mechanism within tha living cells and organs. Xt is also hoped that this information may ultimately help in batter pharmacological control of cellular functions, toltuonshlp of promoted reeearch to tha laboratory^ long term coslsi These studies will be part of a bigger research program on i tha regulation of MtA synthesis within animal cells,with spe cial emphasis on MtA synthesis in central nervous system in vivo and in vitro. I I'll Hi 'UlP*JW 1 - ,,B!,.!-I`WJI-IM II *!!'V^PipiPlIPPiP^i LIA12318 iiiir>^~iii^iiiTittfi ' -| fltiii"MMfac, iMjenma itiirifasll-' BIOGRAPHICAL 8XS7CH _ H.M.Sobkoeics ^ Hanna N. Sobkoeica.M.D. Borni 1,1,1931. Warsaw - Poland Aaalatant Professor of neurology, University of Wisconsin Hadleal School,Tissue Culture Laboratory of the Department of neurology, Chairmem frencis H. forster.M.D. Sept. 1, DM - to date Professional experience* felloe of national Multiple Sclerosis Society at Columbia Univeraltyi Ubo ratory for Cell Physiology - Dr.M.R. Murray, Peb.1.1943 - Sept. 1,1944 Mount Sinai Hospital,M.Y.C.t Laboratory of Cellular neurophysiology, | Dr.M.B.Bornsteln, Research felloe at the Dept.of neurology( Dr.H.Bender,Mt.Sinai Hospital, n.Y.C.tLaboratory of Cellular neurophy siology. Dr. H. a. Borns tel n. Sept.32,1963- feb.1,1943 see Senior Assistant ( neurology.neuropathology) neurological Clinic of Medical Academy in Warsaw - Poland, Dr,X.Newsman. e Sept.1,1941 - Sept.31,1943 Junior Assistant in neurology, neurological Clinic of Medical Academy * in WarsawPoland,Dr. J.Hauemen, Sept. 1,1939 * Sept,1,1941 1 "Mm* ii M ' ' '.III H&gl." ai!l.iwipj||l|wwp(jjppp HP LIA12319 .BIOGRAPHICAL SKETCH f-rWnwftr-f wrem -a- g*Slgl Girls State Lyceum, Olivice- Poland Baccalaureat see Medical Aeidaqr In Warsaw - Poland Medical Doctor *# let Internal Medical Clinic of Medical Academy in Warsaw - Poaland Intern ** neurological Clinic Of Medical Academy in Warsaw - Poland Sealdent tel '..... 'ls*: ' -'-"-1 H.M.SobVowica 1943 - 1949 1949 - 19S4 1954 - 1955 1955 - 1959 Board in neurology i Sept. 39.1959 Medical Academy of Waraaw - Poland Equivalent of Ph.D. in Medical Sclancei 1943 Medical Academy of Warsaw - Poland; Thesis fro* the Department of neurology { Dr.l.Kausman) " The neurologic*1 Syndromes Due To Lead Poisoning* Personal Publications < related to lead poisoning)! 1." H.M.BobVovici, The Ksurological Syndromes iX>e To Lead Poisoning. Those da Doctoral an Medicine. Medicsl Academy of Warsaw - 1443. ( in Polish) ..j j ^ip ij w ip p i.Jn^mn.uijPH11liii^iipppw^ijpiiwpii^wiljijpi? LIA12320 ir p &dikacuJUabiat BIOGRAPHICAL, SKETCH 'itiirthih -3- I SS!* > mestia Mihi iJ H.M.SobVovics 3.* Z.FaUtowsXa.H.M.Sobkowics,j.Tur. Chronic Load Poisoning with Ophthalmic and Cantral Harvoua Syataii Danage. Arch.Malad.Prof., 1961,22(12),759-761. ( in Pranchl Pol.Tyg.Lek. 1964.19(1) 12-15 ( in Poliah) 3. - B.Adanovici, T.DoryvalaXi,J.Kus.H.Sobkowlcs. Chang** in th* Labyrin thine - Vaatibular System in Chronic Lead Poisoning. VX Bat.Conf.Znd.Med.,Gdansk - 1962 ( in Poliah) 4. - Z. Heusawnowa-Petrvsevlcs.B.Emeryk.H.SobXowlcs.B.Wasowics,J.Tur. EMC in Chronic Load Poisoning. ( in Poliah) Pol.Tyg.Lek. 1962.17,36. 5. - B.M.Sobkowies.M.It.Murray.L.J.Goldvater.E.R.Peterson,M.B.Borostein. Influence of Lad Ac*tat* On Development and Maintenance of Rervoua Tissue in Culture. Manuscript in preparation. 4.- H.M.Sobkowica,E.R.Peterson,M.R.Murray, L. J.Coldwater. Absorption of Lead Acetat* by Kervous Tissue in Cu)tur Manuscript in preparation. 7.- H.M.fobkowics.M.R.Murray, L.J.Goldwa ter, E. it. Peter son, M.B. Boros tein. Influence of Serum from Laboratory Animals with Lead poisoning on Rervoua Tissue in Culture. Manuscript in preparation. ipJJIU. ' _ , IJppH. J j.m IMliWjpiBJm.", UU9Hi mmwimpmm pUHNfl LIA12321 qfipp in|g>1j|^,Mtoi. ----- -a > BIOGRAPHICAL SKETCH H. A. KublnsU PREVIOUS WORK AND INVESTIGATOR`S CAREER Name H. A. XublnaM PflHfllliah January IS, IBS) Marital Status Married, 2 children Plaoa of Birth Waraaw, Poland fits. Mala Education Lycaum, Prustkow/Wersaw, Poland Unlvaralty oi Warsaw Madlcal School sam BS MD Vsar Conferred 19S0 19SS Mftlof Retarch Imwt Molacular biology, neurochemlstry, virology Aataarch And Professional Experience 196$ - Department of Surgery, Division of Neurosurgery, University of Wisconsin Medical School, Assistant Professor. from 19$4-19$$ - research assistant at Medical School Institute of Microbiology, Warsaw, Poland. I9S6-1960 - research associate at the same institute. Research: virus-cell Intrratti-*n, a-iiorrtion and penetration of viruses, 1960-1964 - research essociate at Snftung rur Lilorschuag der apinalen Klnderlaahmung und der Muitiplen S. Iarose (presently: Heinrtch-Pette-Institute et University of Hamburg) Hamburg. Germany. Research on: nucleic acid 1 matabollsa In normal, neoplastic and virus-infected animal cells. Chemistry of virus nucleic acids. 1964-196$ - McArdie Laboratory for Cancer Research, University of Wisconsin, S Madison, Wisconsin, project essociate. Research on: interaction of nucleic acids. The studies related to the proposed program have been pursued since 1960. They included studies on: (a) RNA snd DNA metabolism in normal and neoplastic animal celts In vivo snd in viir_- as well as similar studies on the metabolism oi nucleic acids in bacteria (ref. S,6,9,It, 12,14 and 16). (b) Influence of various factors (virus infection, treatment with antibodies, drugs and Mediation) on the Synthesis of RKA m animal cells (raf, 1.2,), 5,7,1, 10 and 19). (c) Binding of polyribonucleotides on riKispmal RNA: conditions of such binding and detectability of complexes (ref. 12 and 18), wmfmm.mmmmm-.. pip'!wpippj mi iii..jiiJiwwiWi^iiiBgtpBii LIA12322 ***-*fmjlfi**- -v ... . BIOGRAPHICAL SKETCH , ... ....... mamMmUUtti^ iniiiWiiffiitfunutw 4 , f*-- > H. A. Kubinski (d) Separation of DNA strands and reactions between DMA and synthetic polyribonucleotides and between DNA and ribosomel ribonucleic acid (ref. 12, 17, 19 and 20). t (a) Methodology of extraction, separation and physico-chemical analysis ti of cellular nucleic acids (ref. 2,4,$,7,12,12 and 17). JgrPKEKCB (0 H. Kubinski and G. Koch: On the Separation of Poliovirus RNA from Cellular 1 Ribonucleic Acid. Virology 12, 220, 1962. (2) H. Kubinski, G. Koch and O. Drees: IVactionauon of Ribonucleic Acid from Poliovirus-Infected Cells by Column Chromatography. Blochiia. Btophye. Acta jji, 322. 1962. (3) H. Kubinski: Chromatography of RNA from Ant lixxty-pius-complement treated Amnion Cells. Symposium on Experimental Contributions to the Pathogenesis of the Domye I mating EncephalomyelitiOes. Hamburg, August 2-4, 1962, 12. Immuniiaets. Allergieforschung, 126. 164, 1964. (4) C. Koch and H. Kubinski: Column Chromatography Separation of Infective and Non-Infective Nucleic Acids from Poliovuus. 2. Neturf. 17b, 6SS. 1962 (in German), (5) H. Kubinski and G. Koch: Instability of an ANA-fraction bom Poliovirus infected Cells. J. Mol. Biol. 6, 102. 1963. (6) H. Kubinski, C. Koch end B. Iltrronyml: Influence of Nutritional Conditions on the Cellular RNA Metabolism in vivo and in vitro. Expertantia (Basal) H, 311, 1963. (7) M. Kubinski: Effect of Vlue-violet Irradiation of Ribonucleic Acid on its Chromatographic Behaviour. Nature (London) 200. $9$, 1963. (9) G. Koch end H. Kubinski: Nucleotide Composition of RNA Synthesised in Human Celia Infected with Poliovirus, Biochemical Symposium Strasbourg, August 19-21, 191); in Bull. Soc. Chim. Biol. 19), 1964 (m Trench. (9) H. Kubinski and G. Koch: On the Synthesis of RNA in Animal Cells under Suboptlmel Good moos, ibid,, 4i, 194, 19(4 (in Trench. (10) G, Koch end II. Kubinski: RNA with s High Cytosine and a Low Guanine Content in Poliovirus Infected Cells, Bloc him, Btnphys, Res, Commons, ii, 306, 19(4. uj mum 'VMSW me* iLwiauian ne n.sese mmm --WMpi LIA12323 ^a^aji^dh<aMwiJS*iii^iritifc1iifci '^iwnwatrtfc. ' IIOCW^PHICAL SKETCH * .* K. A. Kublnakl 00 G. Koch and H. Kublnakl: Synthesis of High Molecular Walght RNA In Mammalian Call*. Vlth Int. Congress of Biochemistry, New York, N.Y. July 2$ - Aug. 1, 1964. Abstracts Vol. h p. 66. 02) Z. Opera-Kublnaka, H. Kublnakl and W. Ssybalskl: Intaractlon batwoan Danaturad DNA, polyrlbonuclaotidas and Rlboaomal ANA: Attampta at Preparative Separation of tha Complamantary DNA Strands. Proc. Natl. Acad. Set. U.8., ii, 923, 1964. 03) G. Koch and H. Kublnakl; On tha Preparation and Fractionation of High Molacular Waight RNA. Z. Naturf. 19b. 663, 1964 On Canaan). 04) G. Koch, H. Kublnakl and G. Tram a: Synthaal* and Turnover of Ribonucleic Aclda in Mammalian Celia in Suapenalon Culture. 1. Influence of Metabolic State. Z. Naturf., 21b. 261, 1966 (inGerman). 03) G. Koch, G. Tram* and H. Kublnakl: Syniheala and Turn-over of Ribonucleic Aclda In Mammalian Celia in Suapenalon Culture. 11. Influence of Actlnomyctn. Z. Naturf. 21b. 967, 1966 On German). 06) H. Kublnakl and G. Koch: Regulation of tha Syntheala of Ribonucleic Aclda In Animal Call*. Biochem. Btophy*. Rea. Commune., 22. 366, 1966 and 1. C. G. 67, Mamo 6216. 07) H. Kubmaki, Z. Opata-Kuhintka and W. Szyhalakl: Pattern! of Intaractlon between Polyribonucleotide! and Individual DNA Strands Derived from Several Vertebrates, Bacteria and Bactenophegaa. J. Mol. Biol., 2fl. 313, 1966 and I. C. G. 67, Mamo 9)47. 06) H. Kublnakl, Z. Opera-Kubinska and W, Szybelski: Binding of TeatLabeled RNA to Ribotomel RNA. Manuscript In preparation. Of) W, Szybaltkl, H. Kublnakl and P. Sheidrick: Pyrimidine Clusters on the Transcribing Strand of DNA and their Possible Role in the Initiation of RNA Synthesis. Cold Spring Harbor Symposium on Quantitative Biology, 31. 1966 (in press). (20) H. Kublnakl and J. A. Rosei Presence of Sequences Containing Repealing Bass-fairs in DNA from Soma Oncogenic and Non-Oncogenic Animal Viruses, Proc. Nat). Acad. Bet, U.S., (in preaa). mmmm. mmm mmgmm LIA12324 i * % ** 1 i 'i BXOORAPnXCAL SKETCH ........ -' --------- ----- ------ ' R.Montain Rachel Monaein,B.A.,R.R.j Bomi 12,4,1928, in Paris, Prance. Project Assistant of Tissue Cslture Laboratory of the Department of Neurology,Chalrmam rrancis M.Forster,N.D. Onlverelty of Wisconsin Medical School. August 1,1967 - to date Professional eyperiencn. Laboratory for Cell Physiology, Columbia University, Dr. M.R.Murray. Research Worker 1966 Laboratory of Cellular Neurophysiology Mt.Sinai Hoepltel,*.Y.C.,Dr.M.B.Bomstein. Research Assistant see Bopitaux de Paria.Bretonneau,Paris,France. Dr. Dreyfua-Brlesec. EM Assistant Mopitaux de Paris.Boucicault Assistance Pub11gue.Dr.Feutheei1. X-Ray Technician 19S9 - 1966 1956 - 1959 1954 1955 Mopitau* de Parle,Cochin Assistance Publlque, Dr.J.Ovenu. ftegisfred Nurse 1951 - 195J Mu c h lows College Sophie Germain,Paris,Prance. Raccalaureat 1946 l . i iiiiu jp)pmnpi|ppp mm r LIA12325 id BIOGRAPHICAL SKETCH -3 Ecole Da la Salpetriere School of Ruraing .Paris,Trane*. Registered Rura* c. ; > * i Ecole da la Salpetclerei ( X-Ray Technical School SCO Technical School *** '1 Tiesue Culture of The Mervous Systaa. Laboratory for Call Physiology. Dr.Margaret R.Murray f Colua&la Univarsity irlTi*frR.Monsain 1951 1951 1955 1959 ppnpffffp