Document ExjVXV7ZrezL1RyE6y4rMOQNx

fin 2 .6 _ O Z & TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE_______________________________________ ___ Identity: A mixture containing perfluorooctanesulfonate; may also be referred to as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC206CF. Current information indicates it is a mixture of 0.5% PFOS, 10% diethylene glycol monobutyl ether, 78.08% water, 1.375% acrylic foamer, 1.5% sodium octyl sulfate, 1% sulfonated alkyl ether salts, 6.0% urea, 0.5% triethanolamine, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: OECD Guideline 201 Type: Acute Static GLP: No Year study performed: 1991 Species: Selenastrum capricornutum Source: In-house culture. Originally from the Culture Collection of Algae at the University of Texas at Austin, Texas. Element basis: Algal cell counts (cells/ml), growth rate Exposure period: 96 hours Statistical Methods: Statistical analyses were performed using the computer program of Stephan, 1983. Analytical monitoring: pH, conductivity, and temperature Test Conditions: Algal Nutrient Medium: Nutrient medium per U.S. EPA, 1978. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 8.0. Stock and test solution preparation: A primary 1000 mg/L stock solution was prepared in algal medium, and added directly to the exposure vessels. Exposure vessels: 250 mL Erlenmeyer flasks containing 100 mL test solution and capped with inverted glass beakers. Agitation: Continuous Number of replicates: 3. Initial cell loading: 1.0 x 104 cells/mL. Number of concentrations: five plus a blank control 001899 Lighting: Continuous lighting at 44 pEin/sec/m2 using cool-white fluorescent lamps. Water Chemistry: pH range (0 - 96 hours): 8 .0 -1 0 .8 (control exposure) 8.1 - 8.3 (1000 mg/L exposure) Test temperature range (0 - 96 hours): 21.9 - 22.3C (incubator) RESULTS_________________________________ Nominal concentrations: Blank control, 62.5, 125, 250, 500, and 1000 mg/L 96-Hrs. Algal Growth Response values Nominal Exposure Cone. (mg/L) Control 62.5 125 250 500 1000 Cell-Count (cells/mL) 602,000 632,000 353,000 304,000 7,000 0 Avg. Specific Growth rate 0.043 0.043 0.037 0.036 -0.003 0.000 % Change from Control -- 0 14 16 >100 >100 Element values: 96-hour ErC50 = 254 (233 - 277) mg/L 96-hour NOEC = 62.5 mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not conducted. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test substance exhibits a 96-hour ErCso of 254 mg/L with a 95% confidence interval of 233 to 277 mg/L. The 96-hour No Observed Effect Concentration (NOEC) was 62.5 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 D A IA-Q.UALITY_______________________________________________ 001900 Reliability: Klimisch ranking = 2. The study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. BEEERENGE2________________________________________________ This test was conducted by EnviroSystems Division, Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-4, 1991. OTHER______________________________________________________ Last changed: 6/28/00 001901 001902 BEST COPY AVAILABLE I. SUMMARY The scut toxicity of FC-206CF to tha alga, Seienastrum capricornutum, la daacribad in thia final raport. The teat waa conducted for 3M Company for 96 hours during July 15 to 19, 1991, at the EnviroSystems Dlvlalon of Resource Analysts, Inc. in Hampton, Hew Hampshire. It was conducted by Jeanne Magazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Timothy Hard. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a mean temperature of 22.1*C. The dilution water waa synthetic media prepared with .sterile deionized water. Flasks were incubated on a rotary shaker, bight waa adjusted to 44 uSln/sec/or with a photoperiod of 24 hours light and 0 hours dark. Nominal concentrations of FC-206CF were 0 mg/L (control), 62.5 mg/L, 125 mg/L, 250 mg/L, 500 mg/L, and 1,000 mg/L. Nominal concentrations were used for all calculations. Algae used in the teBt was from an in-house culture that was maintained under test conditions. Exposure of algae to the test substance resulted in a 72 hour EC50 of 203 mg/L FC-206CF and a no observed effect concentration (NOEC) of 62.5 mg/L. The 96 hour RC50 and HOEC are 254 mg/L and 62.5 mg/L, respectively. BEST COPY AVAILABLE II. T A B U OF CONTENTS SECTIONt I. Sunmary II. Tabla of Contanta III. Index of Tablas IV. Introduction V. Kathods and Matarais VI. Results VII. Refarencas Appendix A . H a t e r Quality Data fro Toxicity Test PAGE 12 13 -'.V. r. BEST COPY AVAILABLE III. INDEX or TABLES Table 1. Chemical characterization of a representative ample of deionised water used to prepare teat media for toxicity teat Table 2. Growth data from toxicity teat 1, ,.~V.. j - *r- Table 3. Percent change from control and average specific growth rate from toxicity teat Table 4. Median effective concentrations (ECSOa) from toxicity teat Table A.l. Temperature measured during toxicity teat Table A.2. pH measured daring toxicity teat BEST COPY AVAILABLE IV. INTRODUCTION Thla study was sponsored by 3K Company, Bt. Paul, Minnasota. Tha objective of tha study was to determine tha acuta toxicity of PC-206CP to * freshwater alga. Tha raport contains ssetions that describe tha methods and materials amploysd in tna study, and tha rssuits of tha investigation. Tha report also contains an appendix that presents tha water quality data collected during tha test. V. METHODS AND MATERIALS TBST SUBSTANCEl PC-206CF (EnviroSyatems Sample Number 2938E) was delivered to SnviroSystems on Kay 23, 1991. It was contained in a 4 os. plastic bottle that was labelled with tha following information: "J1884-4, FC-206CF". FC-206CP (a light brown liquid) was supplied by 3H Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use tha test material was stored in the dark at room temperature. A reserve sample (approximately 1.0 gram) will be retained at SnviroSystems for a minimum of 10 years. DILUTION HATER: Hater used for acclimation of test organisms and for all toxicity I ..a m I 1a J m fl A #n m seem e a e a i . .1 aM Table 1. Chemical characterisation of a representative sample of deionised water used to prepare test media for toxicity test Parameter ' Onit of Measurement Reporting Limit Measured Value Organochlorine pesticides ug/L 2 HD Organophosphorus ug/L 0.5 HD pesticides Polychlorinated biphenyls ug/L 0.5 HD Itotei h d " not detected at or above the reporting limit. BEST COPY AVAILABLE The test was conducted at a target temperature of 22 t 2*C with five concentrations of teat substance and a dilution water control. A 1,000 mg/L stock solution was prepared In dilution water. The stock solution was added directly to dilution water contained in the test vessels without the use of a solvent. Mooinai concentrations of the test material were: 0 mg/L (control), 62.5 mg/L, 125 mg/L, 250 mg/L, 500 mg/L, and 1,000 mg/L. Algae was randomly and equally distributed among three replicates of each treatment at the rate of 10,000 cells/ml. The test was performed in 250 ml glass Krlenmeyer flasks that contained 100 ml of test solution. The flasks were capped with inverted glass beakers. Test vessels were randomly arranged on a rotary shaker in an Incubator during the 96 hour teat (a random numbers table was used to select the location of each vessel). A 24 hour light and 0 hour dark photoperiod was -maintained with cool-white fluorescent lights that provided a light Intensity of 44 uEg-1m~ . The number of algal cells/ml in each test vessel was determined visually every 24 hours by means of direct microscopic counts with a hemacytometer. The pH (Beckman model pHI 51 meter) was determined In each test flask at the beginning and end of the test, and temperature (ASTM mercury thermometer) was measured and recorded daily in the incubator. STATISTICAL METHODS: Results of the toxicity test were Interpreted by standard statistical techniques (Stephan, 1983). The binomial or moving average method was used by the author to calculate 8C50 values using nominal concentrations of test substance, ;- The MOBC was calculated using Dunnett's test, which includes a parametric one-way a n a ly s i s of variance (ANOVA). The average specific growth rate was calculated as the natural log of the number of cells per ml at 24, 48,$72,:or 96 hours minus the natural log of the number of cells per m l a t O hours divided by the exposure period. *;""The*percent change from "control .is calculated by" subtracting the. sample .average specific growth'rate from ,v:the.: control average .'specific' growthCrate, dividing that,value by, the $ P k v '- average specific growth rate in the control/and multiplying that value BEST COPY AVAILABLE ii VI. RESOLTS Biological and watar quality data ganaratad by tha acuta toxicity taat ara peaaantad in Tabla 2 and Appandlx A, raspactivaly, and tha paccant of control and avaraga apacific growth rata Information ia prasantad in Tabla 3. Tha 24, 48, 72, and 96 hour ECSOa for algaa axpoaad to FC-206CT ara prasantad in Tabla 4. Tha 72 hour ECSO la 203 mg/L, and tha 72 hour NOEC is 62.5 mg/L FC-206CT. Tha 96 hour EC50 is 254 mg/L and tha 96 hour NOEC ia 62.5 mg/L. ,5''k'Pj'''t0--..> .\ / .j,.(\,'J?`,,.. V ` ; BESTTabi* 2. Orcwth data fro toxicity t*at c o p y a v a il a b l e Noainal (ag/L) rap. !tub*r of Calla par Millilitar x 103 (hour) 0 24 48 72 96 0-0 (control) 1 2 3 Man 62.5 1 2 M3a n 125 1 2 3 Man 250 *"v: r*'s-.: r,\.- ; ,Jl. sob *$.-V 1 2 M3a n 1 2 3 maan 12 .,3 W/iMm?^an 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 . 10 28 128 28 116 38 146 31 130 310 246 288 281 620 512 674 602 26 105 26 95 32 85 28 95 220 208 228 219 654 690 552 632 20 65 84 354 24 55 116 350 22 60 110 356 22 60 103 353 20 50 114 426 28 35 74 218 22 35 70 268 23 40 86 304 20 10 20 6 10 10 8 6 12 14 .4 8 14 -li..: il .7, .`t . .. --r>1 rj.-; - io * ft'*(.<' 12 ,Jr V il4 V5$ ' i6 g - VfLiozdl* 13 -n ..*.-....... 0 -.'o n 0 0 _ :;n~ _o 7-^fcV.V''' c t & j -?! Vv>tr Vv' ;v i 't'i?*' ;Vi.' I -/w 001910 Tabi 3. Prcnt chang* froa control and avarag pacific growth rat from toxicity tst . y \>i I Nominal Avrag pacific Percent change Concentration ' growth rat from control of Test Substanc 5 (ag/I.) 24hr 8hr 72hr 96hr 24hr 48hr 72hr 96hr 0.0 (control) 62.5 125 250 500 1,000 0.048 0.054 0.046 0.043 0.043 0.047 0.043 0.043 0.033 0.038 0.032 0.037 0.035 0.029 0.030 0.036 0.014 0.002 0.001 -0.003 0.011 0.000 0.000 0.000 00 0 0 10 13 6 0 31 30 30 14 27 46 35 16 71 90 98 >100 77 >100 >100 >100 BEST c o p y a v a il a b l e '^5>-r-V'v -r* -* .. -. - S f S a K e ", * > i^ * S ? li,v ***$*$,; `r ^ v > '* S - - i f f c -f i0iof;15i 001911 Tabi 4 Madian ffactlva concentrations (SCSOs) from toxicity tsat -Exposure period EC50 95 percent confidence Units Calculation method 24 hours 332 mg/L 279 - 403 mg/L 48 hours 199 ag/L 177 - 223 ng/L & 7 2 hours 96 hours 203 ng/L 2S4 mg/L 183 - 225 g/L 233 - 277 ng/L ;'Ciris. ;' 1'*> *;: - BEST COPY AVAILABLE . " -liiA Moving Average Moving Average Moving Average Moving Average vii. m r u m c z s OZCD. 1984. Guidelines for Tasting of Chaaicals. Section 2 Iffacta on Biotic Systems. Mathod 201, Alg. Orowth Inhibition Teat. Adopted June 4, 1984. Stephan, C.B. 1983. Computer program for calculation of LC50 values. Personal communication. O.S. SPA. 1978. The Stlmn&Mtrua caprlcornutun Prints Algal Assay Bottle Teat. BPA-600/9-78-018. July, 1978. BEST COPY AVAILABLE w.A'>WL> tZ iitfA m i* U i  r r . ;t" ,;-T- Tabi* Al Day of Ixpoaur# 0 1 2 34 Tamparatura of Incubator,*c 21.9 22.1 22.1 -22.0 22.3 ......JgJ. '--if. -A,. - >^ r M f e `} - * * - '. n - \ ` ......... '. .'^ M m k ^ 0 \ , \ 001915 BEST COPY AVAILABLE T*bl A. 2. pH M u u r t d during toxicity t u t Roatinal concentration Cg/L) 0 (control) 62.5 125 250 S00 1,000?'.= ' \ &A Raplicata pH initial final 1 2 3 1 . 2 3 1 2 3 1 2 3 1 v 2 3 \ 1 2 %,.3-s i ^ 8.0 8.0 8.1 . 8.3 - 8-.3 8.3 8.3 8.3 8.3 ` ; ; ' . U' - .. , '' a >i 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 10.8 10.8 10.6 9.6 9.6 10.0 9.3 9.3 9.1 9.3 9.1 8.8 8.4 8.4 8.3 8.2 8.2 8.1