Document ExV8zg054BBx7zewvyejJmaZj

. AR LR6 - 0907 MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: tMoixatsurPeFcOoSn,taFinCi-n9g5,PeorrfalusoraococotmapnoensuelnftonoafteF;C-m30a7y2.als(o1-be referred Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-3072. Current information indicates it is a mixture of 1.67% test substance, 33.33% diethylene glycol butyl ether, 29.23% water, 20.83% urea, 5% sodium octyl sulfate, 3.33% sulfonated alkyl ether sulfate salts, 4.60% acrylic foamer, 1.67% triethanolamine, and 0.33% tolyl triazole. The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with thatofthe fluorochemical component. METHOD: Method: Microbics Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1995 SAnpaelcyiteisc:alPmhoontiotboarcitnegr:iupmHphaonsdpohsomroetuicm condition. Exposure period: 30-minutes Statistical methods: ECS50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Millipore Mill-QTM water TSetsotckteamnpdertaetsutrseo:luNtoiotnnpotreedp.aration: A 2000 mg/L primary stock sMoillultipioorne wMaislplr-QeTMparwaetderb.y Adissseoclovnidngar2y00stmocgk test substa solution of nce 520 in 100 mg/L mL was prepared by diluting 26 mL of initial stock solution with water. The pH of the stock solution was 6.8. An osmotic adjustment was made using 200 mg NaCl dissolved in 10 mL stock solution. All test solutions were made by proportional dilutions Exposure vessels: 4 mL cuvettes Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss 002935 RESULTS Nominal concentrations: Blank control, 32.5, 65, 130, and 260 mg/L Element value: 5-minute ECso = 305 (282 - 330) mg/L. 15-minute ECso = 184 (177 - 192) mg/L. 30-minute ECs = 139 (134 - 144) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as dtheastcrmiibxetdurien atnhed tneostt tshuebfsltuaonrcoechreemmiacraklspfrioepldo.rtTiohneavloanleu:es reported apply to CONCLUSIONS - The test substance 30-minute ECs for Photobacterium phosphoreum was determined to be 138 mg/L with a 85% confidence interval of 13410 144 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331,St. Paul, MN, 55133 DATA QUALITY. Reliability: Kiimisch ranking = 2. This study meets the criteria for quality tleasctkisnga.nalHyotwiecavlerc,onsfiarmmpalteiopnuroiftythweaasmnooutnptroopfefrlluyorcohcahreamcitcearlizperdopaonrdtitohneisntudy solution. REFERENCES TMhiinsnesstoutday,wLaasbcRoenqduuecsttednubmybtehre M33M59En7v,ir1o9n9m5e.ntal Laboratory, St. Paul, OTHER Last changed: 6/25/00. 002936 06/25/2000 EANRVCIHRIOVNEMDENTFAILNALLABROERPAOTROTRY 1 LAB REQUEST NO. REQUESTOR NAME: M3597 SBS/KEN GOEBEL DEPARTMENT: 3058 PROJECT NO: BILTHENVIR DATE RECEIVED: 11/29/1994 DESC: FC-3072 AFFF BASE CONTRACT LAB(S): ASCI EXP COMP DATE: 02/06/1995 DATE COMPLETED: 03/24/1995 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 aM FAX NO: 651-778-6176 TESTING IS TO BE DONE IN-HOUSE. sample sent to contract lab for toxicity to fish evlauation due to problems with lab fish cultures. SAB 2/20/95 Results from Microtox testing: Test Conc. mg/L 32.5 85 130 260 Percent Light 5-min exp. Loss as Compared 15-min exp. to the Control 30-min exp. 10 12 15 16 27 21 a7 27 47_ a6 62 74 EC50 305 184 139 sab 3/16/95 Results from Fathead minnow After 96-hours of exposure, toxicity all test test: fish were still alive in 1000 mg/L test substance. SAB 3/22/95 Activated Sludge After 3-hours of respiration inhibition test results: exposure, there was 15% inhibition in respiration at 1000mg/L SAB 3/22/96 Results from No mortality Daphnia magna toxicity test: or immobilization was observed at 1000 mg/L after 48-hours. SAB 3/23/95 SAMPLE DATE CODE DESCRIPTION RESULT MDL or (95% C.I.) 1 FC-3072 LIQUID 48-HR-EC50 DAPHNIA ACUTE TEST ASCI 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR MICROTOX MICROTOX - EC50 SLUDG-RESP SLUDGE RESPIRATION - ECS50 * = CONTRACT LAB DATA >1000 MG/L >1000 MG/L 139 Mo/L >1000 MG/L -) ((-1)34 - 144) -) 002937 BEST COPY AVAILABLE ENVIRONMENTAL A LABORACTo ORY: RUN MICROTOX PREP SHEET oLnRe Ava 2 Va oo able _Lniresg Ca TEST SUBSTANCE: EC - 372 --1. 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