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A R X & - 0 5 (0
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, FC116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS #3825-26-1)
Remarks: 3M production lot number 390. The test sample is FC-126, a white powdery solid. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 78 - 93% test substance and 7 - 22% C5, Ce, and C7 perfluoro analogue compounds.
METHOD:
Method: OECD 209 Test type: Static acute GLP: No Year completed: 1987 Analytical monitoring: Dissolved oxygen concentrations. Test organism source: Activated sludge mixed liquor collected from the metro Wastewater Treatment Plant, St Paul, MN. Used the same day as collection. Test conditions:
Dilution water: Aerated, distilled, deionized water Synthetic sewage: per OECD 209 Test vessels: Not stated Number of concentrations: 5 plus one reference substance and 2 blank controls. Test temperature: 2 0 - 2 1 C Mixed liquor Suspended Solids: 2.7 g/L pH of Activated Sludge Mixed Liquor: 7.8 (initial) and 8.0 (final) pH of 1000 mg/L test concentration: 7.4 (initial) and 8.1 (final) Exposure period: 30-minutes and 3-hours
Reference and test solution preparation: All test substance solutions were created by adding individual weights to solutions containing 284 mL Dl water, 16 mL synthetic sewage feed, and 200 mL inoculum. The pH of these solutions was then adjusted to 7.0 using 1N NaOH. Reference solutions were created using a 500 mg/L stock solution of 3,5dichlorophenol
C03854
Element basis: Respiration inhibition as determined by dissolved oxygen consumption.
RESULTS
__________________________
Nominal concentrations: Two Blank controls, 100, 180, 320, 560, and 1000 mg/L test material solution.
Percent Inhibition in Respiration Rate
Test Substance Concentrations
(mg/L)
Control I Control II
100 180 320 560 1000
30-Minute Exposure
0 0 1.1 0 7.0 11 29
3-Hour Exposure
0 0 4.2 4.7 14 17 38
Element values: 30-minute EC50 = > 1000 mg/L 3-hour EC50 = > 1000 mg/L
Control response: Adequate - 2.2% difference in respiration rate of the two controls for the 30-minute exposure and 0.8% difference in respiration rate of the two controls for the 3-hour exposure.
CONCLUSIONS
The test material induced 38% inhibition in respiration rate at 1000 mg/L after 3-hours of exposure.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Klimisch ranking 2. This study meets the criteria for quality testing. However, it lacks characterization of the test substance purity and analytical confirmation of test substance concentrations. No results were given for the reference compound.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number E1282. OTHER Last changed: 5/24/00
003356
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
LR i Z & A Date 4 - Z O S I
Type Test
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Date Obtained tj -3*0 -<g7______________ Date Used H -9*0 ~4?*7__________
Mixed Liquor Suspended Solids (MLSS)
, 7 g/L Adjustment kAan-f g/L
pH ^ m j - l d l- 7 .
g.Q
Comments____________________________________________________ ____________
Test Substance Sample Description
F d -lZ U
L c \ 3 ^ 0 ________________ ______
Sample , Comments JTk a ^ A u * 1
,,^Adjustment /V>I
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______________________________________
Reference Substance
Sample Description 3- , - foi,Vilo r e p h g n o 1_________________________
Sample pH 7< 3 _________________ Adjustment Jon +
Comments .Stork.
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-----------------------
Exposure Conditions
Test Duration
'Pi -
Test Temperature .2 - <P 1 C
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C03857
Form 1M 20-8-B PWO
Lab Request No. I
Environmental Lab --Work Sheet
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C03858
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ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST OECD #209
\
RAW DATA
M ga, P& 2 Cfc 3
Test Substance: F C - ! 1 X L _____ L a
O ________________________________________
Source of Activated Sludge Mixed Liquor: M 0 ires U.ViCte TOP aiWrrt- P U r t h
Date Received:
H ~ 2 C - g T ___________ Date Usedj_____________________________
Analyst:
__ Q t ( P fa
30 - MIN. EXPOSURE
Test Substance Concentration (mg/L)
CONTROL I
lo o IRC* 3,9-0 37b D / O OCj
0.5
>>b
DISSOLVED OXYGEN CONCENTRATIONS mg/L
TIM!B-MINU1Fes
1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0
cS/ b. 0\ 5 & 4*8 .1 3 3 a .y I.6! / . A .
s s $ .5 H-S. HA 2 A X I A.o 1-3 o>
5 ,3 v .i
S,*)
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O.b
o.sy^
ST.tQ
oA oSlS\3/\
5 .5 3>X f S 37\ 3 - 3 2.1 9.A i s 0 3
i , 1 5 . 3 5 . 5 HA H 'l 3-A 3 -ti 25i 2-H I A
Totof 10.0 C
*|
L\ A) X) Ad
i< r
CONTROL II
4. 7 A
5J
5.o 1 2 3>b 2 f \
2 .x S' on
O.L?/ A 'zo A\
3 - HOUR EXPOSURE
Test Substance Concentration (mg/L) 0.5 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 i*fc
N cr 0 a
CONTROL I
iDCA
/g o
3.Xt> 35b o
7.H 7- X 4 . 7
5 . y 5.| V*? 1-1 3 . 5 3 . 0 I S * 1
4 . 3 5 3 5 - 3 HQ H 3 3 3 3 A 3 2 X A |
3 . 0 7A 7 5 7 . 0 4 . 5 b. | 5 ` b 5.1 . 4 H.\ 3 . b 10
8 .*f s.,3 7.*| 7 5 7. 1 4 . 7 b. X 5-8 5 . 3
1-1 A o
f>0 7.<\ 7 3 7 . X 4 - 7 3 5 5 5 3 m
8 S 83T S3 ?.o 7.7 7 ' i 7 . 1 b .i 4 5
i s i o 9.1 b ,| 5 . 7
CONTROL II
7A 7 5 4 . 7 A d - S J b s j _ I k . H ,o 3 5 '3 .0 3 4 a
pH of Activated Sludge Mixed Liquor Xnvt\a\a 7* &
pH of highest concentration tested
Test Temperature .q ~ <7 I C
InV*uU r 7* ^
So\n. f>re.p
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FirwA* S * I
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ENVIRONMENTAL LABORATORY
D a te
Analys t __
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST OECD #209
CALCULATION OF RESULTS
F CT eat Substance________
- I .3. U
Lei ?><\ O
30 - MINUTE EXPOSURE
Test Substance C o n c e n tra tio n s (me/L)
R XOxygen Uptake Rate
Correct^ In h ib itio n in
An'CR 4d
R e s p ira tio n Rate
CONTROL I
O. 7
0 , t*><\ i ^
--
I OC
O 7083,
O. 1 ,7 ^
M
m. 1LC
o. 7 (q n
. UR7H
?> Ad O.kL, SS~ o . tousle . 7 .0
S'>D
O 5 0 5 (o
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(!
lo c o
o_______
____ O.H8H 8
S'7
CONTROL I I
. 10 8 1
C .L7 3
g g CPCP e rce n t D iffe re n c e in C o n tro ls (2) ,
--.
EC50 (95 Z Confidence Limits) mg/L
T
3 - HOUR EXPOSURE
SbOjIOCoylL 66- Aoio
( i t t o - ^ }si-w o y
T e st Substance C o n c e n tra tio n s Cme/L)
Oxygen Uptake R
Rate
R 4
C3okfCotCHC.
CONTROL I
o.
ICO
C SO SS'
O . 4 ft3 .fi'
(QC A o H & o o
o 1333
c.H
SU O
o . HHoo
o .H .io a ,
lo c ____
___________ ________ o . 3 f
___ o. s i ys '
Z In h ib itio n in R e s p ira tio n Rate (*)
--
i. a 1 .7
14
17
32
CONTROL I I
o.5X<=\l
G . &P e rce n t D iffe re n c e in C o n tro ls (2)
5* 'EC50 (95Z C on fid en ce L im its ) mg/L
5^
.505 1000 m iL
--
(1) Z Inhibition
[BLC + RCA
K + SC2 \
T e m p . Corcec^JL 7 o eC ;
r it^|ipermir).4. * 1*0, - * c j (2) Z D iffe re n c e ^RCj + RC2^ x 100
oJotZ zo)
^
Where: R - Oxygen'.Uptake R ate (mg O ^ /L/M in.)
S - T e st Substance
1 C ontrol I 0 ~ 1.0*7 ,, .
C_- C o n tro l I I
2 coa&G O
* T,cVi -- J a Cl
The two BK C o n tro l r e s p ir a t io n ra te s sh o u ld
be w ith in 15Z o f each oth e r.___________ _ _
