Document EvemxKVg7xyaVjZkqgRmBE120

,,.,)HAZLMMON W IS C 0 N S IN POST OFFICE BOX 75,45 s MADISON. WISCONSIN 53707. 54 a COMWJG LAbolMOIV Se@CeS CMnoo@, Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Titly: 13-Week Dietary Toxicity Study with 7-5180, Ammonium Perfluorooctanoate(CAS No. 3825-26-1) in Male Rats Data Reouirement.: Guideline 82-1 Author: Matthew J. Palazzolo, PhD, DAST Dill: May 17, 1993 Performing Laboratory: HazletonWisconsin, Inc. 3301 Kinsman Boulevard Madison, Wisconsin 63704 LaboratoryProject Identification: HWI 6329-100 VOLUME I OF Il p 0 ne F 0 A E XPA 6 0 a 2 a I A A-7 1 E S S K4 A IL 0 E L Iv E A y Page I of 657 3301 Klt4SMAN SOULE VAFKD F8 IqAol S Oti 6 0 E: 2 4 1 W ISC of4 E 72 2 7 53 704 NWI 6329-100 COMPLIANCE STATEMENT 13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats All aspects of this study that were carried out by Hazleton Wisconsin, Inc., and Hazleton Washington, Inc., were in accordance with the Environmental Protection Agency Good Laboratory Practice Standards, 40 CFR 160. Ma ew J. P21hkzol Diplomate,Aii- 0/1@g, Study Director Director Toxicology Hazleton Wisconsin, Inc. Date To the best of my knowledge, the above statement is complete and accurate. Sponsor's Representative Date 3M To the best of my knowledge, this report is complete and unaltered. Applicant Date 3H 2 This Page IntentionallyLeft Blank HWI 6329-100 3 I i4WI 6329-100 QUALITY ASSURANCE STATEMENT 13-WeekDietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate(CAS No. 3825-26-1) in Male Rats The report for this study,with the exceptionof AppendixE, has been reviewedby the QualityAssuranceUnit of HazletonWisconsin,Inc., in accordancewith the Good LaboratoryPracticeStandardsas set forth in 40 CFR 160.35(b) (6) (7). The report identifiesand/ordescribes the authorizedmethods and Standard Operating Procedures followed in the conductof the study. The QualityAssuranceUnit has also conducted the followinginspectionosf the testingfacilitiesused in the conductof this study and has submittedwritten reportsof these inspectionsto the Study Director. Written status reports of inspectionsand findingsare submitted to Management according to HazletonWisconsin, Inc., StandardOperating Procedures. Date of Inspgction Type of Inspection Date Issued to Study Director 02/20/90 Protocol Review 02/20/90 03/02/90 Protocol Amendment Review 03/07/90 12/27/90 Body Weight Measurement 12/27/90 12/27/90 Food Consumption Measurement 12/27/90 12/27/90 Animal Observation 12/27/90 01/02/91 Test Material Preparation 01/02/91 01/02/91 Sample Analysis 01/02/91 01/02/91 Test MaterialAdministration 01/02/91 01/02/91 PreparedTest Material Dispensation 01/02/91 01/14/91 Protocol Amendment Review 01/14/91 01/21/91 Protocol Amendment Review 01/21/91 01/24/91 Clinical/PhamacokineticSampling 01/24/91 02/12/91 Necropsy 02/12/91 03/25/91 Clinical/PhamacokineticSampling 03/25/91 04/29/91 04/30/91 Data Review Data Review 04/30/91 04/30/91 05/16/91 Necropsy 05/16/91 4 Date of Inspection 05/23/91 07/22-26,29-319 08/01,02,05-07/91 09/13/91 11/18-12/04/91 11/20-12/04/91 09/28/92 02/19-24/93 03/23/93 04/08/93 QUALITY ASSURANCE STATEMENT Type of Inspection Histology Operation Data Review Special Analysis Data Review Report Review Protocol Amendment Review Report Review Protocol Amendment Review Report Review NWI 6329-100 Date Issuedto Study Director 05/23/91 08/07/91 09/18/91 12/05/91 12/04/91 09/28/92 02/24/93 03/23/93 04/08/93 ohrt--0B-o 111ng Representative,QualityAsvrance Unit E--Ll -7 @9 3 Date HWI 6329-100 STUDY IDENTIFICATION 13-Week Dietary Toxicity StUdy with T-5180, Ammonium Perfluorooctanoate(CAS No. 3825-26-1) in Male Rats Test Material Sponsor Study Monitor Study Director Study Location T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000 Roger G. Perkins, PhD, DABT 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000 Matthew J. Palazzolo, PhD, DABT Hazleton Wisconsin, Inc. P.O. Box 7545 Madison, Wisconsin 53707 (608) 242-2622 Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704 Study Timetable In-Life (ixperimentalS)tart Date In-Life TerminationDate Experimental Termination Date December 20, 1990 May 17, 1991 May 17, 1993 6 HWI 6329-100 KEY PERSONNEL Toxicology Matthew J. Palazzolo, PhD Diplomate, ABT Study Director Director Janice D. Maneke Study Coordinator Toxicology Operations Larry Giltner Group Leader Sample Collection Robert K. Young Group Leader Rodent Toxicology Shane Eith Group Leader Support Services Compound Preparation Dixie Bushee Group Leader Laboratory Animal Veterinarian Cindy J. Cary, DVM Diplomate, ACLAM Clinical Pathology Robert L. Hall, DVM, PhD Diplomate, ACVP Clinical Pathologist Clinical Pathology (Continued) Ronald Markevitch Supervisor Anatomical Pathology Thomas E. Palmer, PhD Anatomical Pathologist Dennis Hoffman Group Leader Necropsy Richard Becker Group Leader Histology Anne Mosher Group Leader Pathology Data ' Aor icultural Chemistry Mandla A. Tshabalala, PhD Manager Methods Development Imunochemistry Joanne Satyanarayan Supervisor Ouality-Assurance Sherry R. W. Petsel Manager 7 CONTENTS VOLUME I OF 11 COMPLIANCE STATEMENT QUALITY ASSURANCE STATEMENT STUDY IDENTIFICATION KEY PERSONNEL ABSTRACT OBJECTIVE REGULATORY COMPLIANCE MAINTENANCE OF RAW DATA, RECORDS, AND,SPECIMENS TEST MATERIAL Identificationand Characterization Storage Conditions Reserve Samples Safety Precautions TEST SYSTEM Test Animal Identification STUDY DESIGN PROCEDURES Dose Preparation Retention Samples Dose Analyses Dose Administration Acclimation Housing and Maintenance Antemortem Observations Body Weights Food Consumption Hormone Analysis Test Material Residue Analysis Clinical Pathology Anatomical Pathology StatisticalAnalyses HWI 6329-100 2 4 6 7 12 14 14 14 14 14 14 14 14 15 15 15 16 16 16 17 17 17 18 is is is 19 19 19 19 20 8 NWI 6329-100 CONTENTS (Continued) Paqe VOLUME I OF 11 (Continued) RESULTS 20 Dose Analyses 21 Acclimation 21 Survival 21 Antemortem Observations 21 Body Weights 22 Mean Body Weight Gains 22 Food Consumption 22 Compound Consumption 22 Hormone Analysis 23 Test Material Residue Analysis 23 ClinicalPathology 23 Anatomical Pathology 23 CONCLUSIONS 24 SIGNATURES 25 REFERENCES 26 PATHOLOGY REPORT 27 COMMENTS ON THE DATA 31 CODES, ABBREVIATIONS, AND UNITS 32 General Codes and Abbreviations 33 Abbreviationsand Units for ClinicalChemistry 34 Codes for Anatomical Pathology 36 TABLE I Summary of Antemortem Observations 38 2 Mean Body Weights and Standard Deviations (g) 41 3 Mean Body Weight Gain (g) 45 4 Mean Food Consumption and Standard Deviations (g/wk) 49 5 Mean Compound Consumption (mg/kg/day) 53 6 Summary of ClinicalChemistry Data - Week 5 56 7 Summary of ClinicalChemistry Data - Week 8 57 8 Summary of ClinicalChemistry Data - Week 14 58 9 Summary of ClinicalChemistry Data - Week 22 59 10 Summary of Absolute Organ Weight Data (g) - 5 Week Sacrifice 60 11 Summary of Absolute Organ Weight Data (g) - 8 Week Sacrifice 62 12 Summary of Absolute Organ Weight Data (9) - 14 Week Sacrifice 64 13 Summary of Absolute Organ Weight Data (g) - 22 Week Sacrifice 66 HWI 6329-100 CONTENTS (Continued) VOLUME I OF 11 (Continued) TABLE (Continued) 14 Summary of Organ-to-Body Weight Percentages - 5 Week Sacrifice 15 Summary of Organ-to-Body Weight Percentages - 8 Week Sacrifice 16 Summary of Organ-to-Body Weight Percentages - 14 Week Sacrifice 17 Summary of organ-to-Body Weight Percentages - 22 Week Sacrifice 18 Summary of Organ-to-Brain Weight Ratios - 5 Week Sacrifice 19 Summary of Organ-to-Brain Weight Ratios - 8 Week Sacrifice 20 Summary of Organ-to-Brain Weight Ratios - 14 Week Sacrifice 21 Summary of Organ-to-Brain Weight Ratios - 22 Week Sacrifice 22 Incidence of Macroscopic Observations - 5 Week Sacrifice 23 Incidence of Macroscopic Observations - 8 Week Sacrifice 24 Incidence of Macroscopic Observations - 14 Week Sacrifice 25 Incidence of Macroscopic Observations - 22 Week Sacrifice 26 Incidence of Microscopic Observations - 5 Week Sacrifice 27 Incidence of Microscopic Observations - 8 Week Sacrifice 28 Incidence of Microscopic Observations - 14 Week Sacrifice 29 Incidence of Microscopic Observations - 22 Week Sacrifice 30 Incidence of Severity of Microscopic Observations - 5 Week Sacrifice 31 Incidence of Severity of Microscopic Observations - 8 Week Sacrifice 32 Incidence of Severity of Microscopic Observations - 14 Week Sacrifice 33 Incidence of Severity of Microscopic Observations - 22 Week Sacrifice 34 Summary of Hormone Data 35 Summary of Test Material Residue Analysis 36 Results of Method Validation for Determinationof T-5180 in Rodent Food Expressed as the Percent of Recovery 37 Results of StabilityAnalysis 38 Results of Homogeneity Analysis 39 Results of Dose Preparation Analysis APPENDIX A Protocol Deviations Protocol TP9321 Protocol Amendment No. 1 Protocol Amendment No. 2 Protocol Amendment No. 3 Material Safety Data Sheet APPENDIX B Individual Antemortem Observations Individual Body Weights (g) Individual Food Consumption (Vwk) APPENDIX C Individual Clinical Chemistry Values paqe 68 70 72 74 76 78 80 82 84 87 90 93 96 99 102 105 108 ill 114 117 120 122 123 126 128 130 132 133 134 147 ISO 152 154 iss 159 177 199 221 222 10 CONTENTS (Continued) VOLUME 11 OF 11 APPENDIX D IndividualAbsolute Organ Weight Data (g) IndividualOrgan-to-Body Weight Percentages IndividualOrgan-to-BrainWeight Ratios IndividualAnimal Pathology Data APPENDIX E Quality Assurance Statement Individual Hormone Analysis Data APPENDIX F Results of IndividualTest Material ResidueAnalyses APPENDIX G Method Validation for FC-143 in Feed Method Validationfor T-5180 in Rat Serum HWI 6329-100 232 233 254 275 295 620 621 622 629 630 644 645 651 HWI 6329-100 ABSTRACT The purposes of this study was to assessthe subchronictoxicityof T-5180, Ammonium Perfluorooctanoate,when fed to rats for at least 13 weeks and to evaluatereversibilityof any toxiceffectsafterapproximately8 weeks without treatment. There were 320 male Crl:CDOBR rats assigned at random to six groups (55/groupin Groups 1 through5, 45/group in Group 6) and fed diets containing 0 (nonpair-fedcontrol),1, 10, 30, 100, or 0 ppm (controlgroup pair-fedto the group fed 100 ppm) for up to 13 weeks. In addition,10 animals/sex/groupin Groups 1 through 5 (designatedas recoveryanimals)were fed basal diet for 8 weeks posttreatment. The animals were observed at least twice daily. Body weights were recorded once before initiationof treatment,weeklythereafter,and on the day of necropsy. Food consumptionwas recordedweekly for animals given 1, 10, or 30 ppm and for the nonpair-fedcontrolgroup. Food consumptionwas recordeddaily for the pair-fed groups (animalsgiven 0 or 100 ppm). Food consumptionwas recorded weekly for all groups during recovery. There were 15 animals/groupsacrificedafter 4, 7, or 13 weeks of treatment; 10 animals/groupin Groups 1 through 5 were sacrificedafter 13 weeks of treatment and 8 weeks without treatment. Hepatic CoA Oxidase activity was determined for five animals/groupat each scheduledsacrifice. Serum samples from 10 animals/groupwere collected at each scheduled sacrifice during treatmentand from 5 animals/groupduring recoveryfor hormone analysis. Nonfasted rats were anesthetizedwith methoxy-.fluranaend blood was collected from the descendingvena cava. These sampleswere analyzed for estradiol,testosterone (total)and luteinizinghormones,and for test materialcontent. One animal given 100 ppm was sacrificedduring Week 4 due to severe neck sores; all other animalssurvivedto scheduledsacrifice. There were no test materialrelated effects on antemortemobservations. Body weight and cumulativebody weight gain for the 100-ppmanimalswere consistentlysignificantlylower than those of the nonpair-fedcontrolgroup throughouttreatment. Animals fed 100 ppm consumed significantlyless food than the nonpair-fedcontrol group at Weeks 1 and 2. Overall,there was no significantdifferencein mean food consumptionbetween the pair-fed and nonpair-fedgroups. Dietaryadministrationof the testmaterialresultedin higherhepaticpalmitoyl CoA oxidaseactivitythat was statisticallysignificantat dose levelsof 30, and 100 ppm at Weeks 5, 8, and 14. Animals given 10 ppm had transientlyhigher hepaticpalmitoylCoA oxidaseactivitythat was statisticallysignificantat Week 5. The mean enzyme activitiesfor animalsgiven 10, 30, or 100 ppm were highestat Week S. No differencein hepaticpalmitoylCoA oxidase activity was seen at Week 22; that is after 8 weeks recoveryfollowingthe 13 weeks of dietaryadministrationof the test material. The effect was dose-dependentand reversible. 12 NWI 6329-100 Administrationof the test materialcaused increasedabsoluteand relativeliver weights and hepatocellularhypertrophyin the liversof animals given 10, 30, or 100 ppm after at least 4, 7, and 13 weeks of treatment. The progressionof hepatocellularhypertrophydid not appearto be affectedby the length of treatment. The changesobservedare suggestive-ofa test material effect on intracellularmetabolismand may be associatedwith peroxisomeproliferation. At the end of recovery,these changeswere not observedin animals, indicatingthat the testmaterial-relatedincreasesin absoluteand relativeliverweightswere reversible. There was no consistentpattern for the hormone determinationswhich could be attributedto the test materialover the entirecourseof the study. None of the homone levels were significantlydifferentbetweengroups althoughthere appeared to be some indicationof elevatedestradiolfor the 100 ppm group at the Week 5 determination. Based on the resultsof this study,the no-observable-adverse-effecltevel for T-5180 when fed ad libitumto rats for at least 13 weeks is 100 ppm; the no-effect level is 1.0 ppm. 13 HWI 6329-100 OBJECTIVE The purposesof this studywas to assessthe subchronictoxicityof T-5180, AmmoniumPerfluorooctanoate(CAS No. 3825-26-1)when fed to rats for at least 13 weeks and to evaluatereversibilityof any toxic effectsafter approximately 8 weeks. REGULATORY COMPLIANCE All aspectsof this studythat were carriedout by Hazleton Wisconsin (HWI) and Hazleton Washington (HWA) were in accordancewith the EnvironmentalProtection Agency Good Laboratory Practice Standards, 40 CFR 160. MAINTENANCE OF RAW DATA, RECORDS, AND SPECIMENS Originalpaper data, magneticallyencodedrecords,and a copy of the final report will be retainedin the archivesof HWI. Specimenswill be transferredto the Sponsor and are to be retained in accordancewith 40 CFR 160.195. TEST MATERIAL Identificationand Characterization The test material, T-5180,Ammonium Perfluorooctanoate(CAS No. 3825-26-1),Lot No. 115, is a light colored powder. It was @eceivedat HWI on November 7, 1990, and was assigned HWI Sample No. OOSSAI. Informationon synthesismethods,composition,or other characteristicsthat definethe test materialis on file with the Sponsor. Storage Conditions The test materialwas stored at room temperature. Reserve Samples Reserve samplesof the test materialwere taken and stored in a freezer. These samples were returned to the Sponsor on December 18, 1991 to be retained in accordancewith 40 CFR 160.195. Any remainingtest material was returnedto the Sponsor on December 18, 1991. Safety Precautions Personnel involvedwith the study wore the apparelrequiredby the MaterialSafety Data Sheet (MSOS) providedby the Sponsor. HWI SOPS applied for any apparel not covered by the MSDS. 14 NWI 6329-100 TEST SYSTEM Test Animal Male Crl:CMR rats were obtained from the Portage, Michigan, facility of Charles River Laboratories, Inc. (Wilmington,Massachusetts),on December 7, 1990. The animals were approximately41 days old at initiation of treatment and weighed 180.7 to 229.1 9. The rat is frequently used in safety evaluation studies as a representativeof a rodent species. Male rats were used to characterizethe effects of the test material on testicular physiology. Identification Each animal was assigned a temporary number upon arrival. Before initiationof treatment, each animal was assigned a permanent number and identified with an ear tag. All data for an animal are recorded under one of these numbers. STUDY DESIGN Animals were assigned to the study using a computer-generated randomization according to the following design: GrouD Dose Level FDRM) Number of Malese 1 0 55 2 1 55 3 10 55 4 30 55 5 100 55 6 ob 45 a 10 animals for Groups I through 5 (designated as recovery animals) were treated for at least 13 weeks, then treatment was discontinued(animalswere fed the basal diet) and they were observed for reversibility,persistence, or delayed occurrenceof toxic effects for at least 8 weeks posttreatment. b Animals in Group 6 were pair-fed with the high-dose animals (Group 5) throughouttreatment. Due to the pairfeeding requirements,the pair-fed control animals (Group6) were initiatedon test one day after all other groups. KWI 6329-100 Individualbody weights at the time of randomizationwere within i2 standard deviations of the mean body weight and group-mean body weights were not statisticalldyifferentat the 5.0% probabilitylevel (Winer,1971). PROCEDURES This study was conducted in accordance with HWI Protocol TP9321 dated November 30, 1990, and Amendment Nos. I through 3. The protocol, protocol amendments,protocol deviations,and MSDS are in AppendixA. Unless otherwise indicated,all procedureswere done in accordancewith the above-referenceddocuments, HWI SOPS, or both. Dose Preparation Carrier. Certified Rodent ChowO OS002 was used as supplied. Test Material. Dietaryconcentrationswere based on the test material as supplied. Diets were preparedweekly. A specifiedamountof carrier (basaldiet) was weighed into a labeledHobartO mixing bowl from which approximately200 g were removed and placed in a Waring blender; the requiredamount of test materialwas then weighed and added to the Waring blender and overlaidwith approximately50 g of carrier,then thoroughly blended;this premix was transferredto the mixing bowl. The contentsof the mixing bowl were mixed for approximately15 minutes to prepare the base diet. Diets were made for Groups 2, 3, 4, and 5 by placing a specifiedamount of basal diet into a labeledHobartOmixing bowl. A specifiedamount of base diet was added to the mixing bowl. The contentsof the mixing bowl were mixed for approximately 15 minutes. Beginningat Week 3, the mixing procedurefor Group 2 was modified. A specified amount of basal diet was placed into a labeled HobartO mixing bowl and a specified amountof the Group 3 diet was added to the mixing bowl. The contents of the mixing bowl were mixed for approximately30 minutes. The diets were stored at room temperature(WeeksI through8) or in a refrigerator (Weeks9 through14) in containersuntil diet administration. Retention Samples Samplesof each mixed batch were stored in a freezerfor at least 6 months for possiblefuture analysis. 16 NWI 6329-100 Dose Analyses Assays for the test materialin the basal diet were done by HWI using an analyticalmethod developed and validated by HWI (AppendixG). Homogeneitywas determinedfor all concentrationsat Week 1. Homogeneitywas also determinedfor the low-dose(I ppm) concentrationat Week 2. Samples taken from the top, bottom, and two opposingsides of the mix were assayed in duplicatefor test materialcontent. To evaluatethe stabilityof the test materialin the basaldiet, four sets of samples were taken from each dose preparationmixed for Week 1. One set was analyzedon the day of mixing,one set was analyzedafter storage for 1 day in a refrigeratorand 7 days at room temperature;and the remaining sets were analyzed afterbeing stored in a freezerfor 2 or 7 weeks. A second stabilitywas done at Week 12 to evaluatethe actual storageconditions during the first eight weeks of the study. Two sets of sampleswere taken from each dose preparation;one set was analyzedon the day of mixing and the second set was analyzed after storage at room temperaturefor 8 days. All diets were assayedeach week for the first 4 weeks. Each week thereafter,a sample of the controldiet and one test materialdietary concentrationwas selected sequentiallyfor analysis. These samples were analyzed as a group approximatelyevery 4 weeks. All sampleswere stored in a freezeruntil analyzed. Dose Administration Dietary admixturewas used to comparedata with other studiesdosed in a similar fashion. The test material was administeredad libitum for at least 13 weeks. After 13 weeks of treatment,10 males (designatedas recoveryanimals) from all groups, except the pair-fedcontrolgroup (Group6), were fed basaldiet for at least 8 weeks posttreatment. Acclimation The animals were received on December 7, 1990, and were acclimated in Animal Room 315 for 13 days beforeinitiationof treatment. During acclimation,the animalswere observeddaily for abnormalitiesindicativeof health problems. 17 HWI 6329-100 Housing and Maintenance Animal Room 315 was used for this study. Environmentalcontrols for the animal room were set to maintain a temperatureof 72*F t6'. a relativehumidity of 50% t20%, and a 12-hourlight/12-hourdark cycle. Variationsfrom these conditionswere documentedand were consideredto have had no effect on the outcome of the study. The animalswere housed individually(exceptfor the first week of acclimation)in stainlesssteel, screen-bottomcages. When animal health status warranted, selectedanimals were individuallyhoused in polycarbonatecages with hardwood chip bedding. Animal husbandryand housing at HWI comply with standards outlined in the Guide for the Care and Use of LaboratoryAnimals (NIN Publ. No. 86-23, 1985). Certified Rodent ChowO #5002 (PurinaMills, Inc.) was provided ad libitum. The lot numbers are recorded. The diet is routinelyanalyzedby the manufacturerfor nutritional components and environmentalcontaminants. Water was providedad libitum. Samplesof the water are analyzedby HWI for total dissolvedsolids, hardness,and specifiedmicrobiologicalcontent and for selected elements, heavy metals, organophosphates,and chlorinatedhydrocarbons. The resultsare on file with HWI. There were no known contaminantsin the food or water that would have interfered with this study. Antemortem Observations The animalswere observedtwice daily (a.m.and p.m.) for mortality,moribundity, and signs of toxicity. At least once weekly, each animalwas removedfrom its cage and a detailed examinationwas done. Body Weighti Individualbody weight data were recordedfor randomization,on the first day of treatment,weekly thereafter,and on the day of necropsy (exceptfor two animals that were not weighed before exsanguination). Food Consumption Individualfood consumptiondata were recordeddaily for animalsthat were pairfed (Groups5 and 6) and weeklyfor all other animalsduring treatment. During recovery,food consumptionwas recordedweekly for all animals. HWI 6329-100 Hormone An1"sis Serum samples were collected from 10 animals/groupat each scheduled sacrifice during treatmentand from 5 animals/groupduring recovery. Nonfasted rats were anesthetizedwith methoxyflurane,and blood was collected from the descendingvena cava and storedon ice until centrifugation.Serum sampleswere stored at approximately-70*C (exceptstoragelocationfor 3 collectiondates cannot be verified),then packed on dry ice, and shippedto HazletonWashington for analysis. Serum sampleswere analyzedfor estradioland testasterone(total)and luteinizinghormones. Test Material Rgsidue Analysis Samplesof liver, testes,lungs, and subcutaneousadipose tissue were collected from all animalsat necropsyand stored frozenfor possibleanalysis. Serum samples collectedfrom up to 10 animals/groupat each scheduledsacrificeduring treatmentand from 5 animals/groupduring recoverywere analyzed for test material residue. Clinical--Patholocy A sectionof liver was obtainedfrom all animalsat each scheduledsacrificeand flashfrozenin liquidnitrogen. A sectionof liver from 5 animals/groupat each scheduledsacrificewas assayedfor the level of palmitoylCoA oxidase as an indicatorof peroxisomeproliferation. Anatomical Patholocty A necropsywas done on the animal that was sacrificedin a moribund condition. After 4, 7, or 13 weeks of treatment,nonfastedanimalswere anesthetizedwith methoxyflurane,weighed, exsanguinated,and necropsied between 7:00 a.m. and 10:00 a.m. (exceptfor three animalsthat were necropsiedafter 10:00). Recovery animalswere sacrificedafter 8 weeks without treatment. The necropsy includeda macroscopicexaminationof the external surface of the body; all orifices;the cranialcavity;the externalsurfacesof the brainand spinal cord; the nasal cavity and paranasalsinuses;and the thoracic,abdominal, and pelvic cavitiesand viscera. At the schedulednecropsy,the followingorgans (whenpresent) were weighed; paired organs were weighed separately: Brain Liver Lungs Testes (onetestis) Accessorysex organs (seminalvesicle, prostate,coagulatinggland, urethra) 19 HWI 6329-100 Organ-to-bodyweight percentagesand organ-to-brainweight ratios were calculated. The following (when present)or representativesampleswere collected from scheduled sacrifice animals and preserved in 10% phosphate-bufferedformalin, unless otherwisespecified: Lesions Brain Liver Lungs Testes (onetestis) Accessorysex organs (seminalvesicle, prostate,coagulatinggland, urethra) The above (as appropriate)were embedded in paraffin,sectioned,stainedwith hematoxylinand eosin, and examinedmicroscopicallyfrom all animals from which they were collected. At the schedulednecropsy samples of the followingtissues were preserved in glutaraldehydefor possibleelectronmicroscopicevaluation: Brain Liver Lungs Testes(one testis) Accessory sex organs (seminalvesicle, prostate) StatisticalAnalyses The statisticalmethodsused are describedbelow. Levene'stest (Levene,1960) was done to test for variancehomogeneity. In the case of heterogeneityof varianceat p s 0.05, transformationswere used to stabilizethe variance. Analysis of variance [ANOVA (Winer, 1971)] was done on the homogeneous or transformeddata. If the ANOVA was significant,Games and IHowellModified TukeyKramer test (Games and Howell, 1976) was used for pairwise comparisons between groups. One-way ANOVA was used to analyze body weights, cumulative body weight gains, food consumption,clinicalchemistry,hormone values, organ weights, organ-to-body weight percentages,and organ-to-brainweight ratios. Body weights,cumulative body weight gains, and food consumptionvalues were analyzed for all groups (except Group 6) using the SAS program according to HWI methods. Group comparisonswere evaluatedat the 5.0% two-tailedprobabilitylevel. RESULTS Groups 2, 3, 4, and 5 were compared with Group 1; Group 6 was compared with Group 5. 20 NWI 6329-100 Dose Analysu Resultsof test diet assaysto confirmmethodvalidation,homogeneity,and stabilityand to verifydose levelsfor T-5180are in Tables36 through39. Methodvalidationresultsindicatedthat the methoddevelopedwas suitablefor the rangeof dietaryconcentratioinn the study. Mean homogeneityresultsfor diets containing30 ppm and 100 ppm were within 10% of the theoreticallevels;theseresultsindicatedthat the mixingprocedure produceda homogeneousdistributionof T-5180in the diet at these levels. Mean homogeneityresultsfor diets containingI ppm and 10 ppm T-5180were within 19% and 18% of the theoreticalvalues,respectively. All test dietswere consideredstableunderthe storageconditionsspecified.The lowervaluesof the theoretical(i.e.,78% for the samplestoredrefrigerated I day, than at room temperature7 days, 75% for the sample storedat room temperature8 days, and 77% for the samplestoredfrozenfor 2 weeks before analysis)wereconsistentwiththe variabilitoyf the assay at the I ppm level. Dose preparationanalysisresultsindicatedthat the appropriatedietary concentrationswere achievedduringthe study. The assay resultsrangedfrom 70.0%-119%,90.1%-116%,87.0%-116%and 90.1%-Ill%of the theoreticaldose levels of 1, 10, 30, and 100 ppm. Acclimation For this study,370 males were receivedand acclimated. In general,animalsin this shipmentappearedhealthy. The animalswere releasedfrom acclimationby the laboratoryanimalveterinarianon December17, 1990. One animalwas excludedfrom studyconsiderationdue to small testes. Therewere 369 males in the randomizationfor assignmentto groups (55/groupfor Groups 1, 2, 3, 4, and 5; 45/groupfor Group6). Animalsnot selectedfor the studywere sacrificedand discardedon December21, 1990. Survival One animalgiven 100 ppm was sacrificedduring Week 4 due to severe neck sores. All otheranimalssurviveduntilscheduledsacrifice. Antemortem Observations Antemortemobservationsare summarizedin Table 1; individualdata are in Appendix B. There were no test material-relatedantemortemobservations. 21 HWI 6329-100 Body Weights Body weight data are summarized in Table 2; individual data are in Appendix B. Body weights for the 100-ppm animals were significantlylower than the pair-fed control group (Group 6) at study initiationand were consistentlysignificantly lower than the nonpair-fedcontrol group (Group 1) throughout treatment (Weeks 1 through 13). During recovery, there were no statisticaldifferences in body weights noted. There were no significant body weight changes for animals at 1, 10, or 30 ppm levels. Mean Body Weight Gains Mean body weight gain data are summarized in Table 3. Mean body weight gains for the 100-ppm animals were significantly higher than the pair-fedcontrol group (Group 6)-at Week I and were consistentlysignificantly lower than the nonpair-fed control group (Group 1) throughout treatment (Weeks 1 through 13). Mean body weight gains were significantlylower than the nonpair-fed control group (Group 1) for animals given 10 or 30 ppm at Week 2. During recovery, there were no statisticaldifferences in mean body weight gains noted. Food Consumption Food consumption data are summarized in Table--4; individual data are in Appendix B. Animals fed 100 ppm (Group 5) consumed significantlyless food than the nonpairfed control group (Group 1) at Weeks 1 and 2. Overall, there was no significant difference in mean food consumption between the pair-fed and nonpair-fed groups. Compound Consumption Compound consumption data are summarized in Table 5. Animals were fed diets containing 1, 10, 30, or 100 ppm of the test material. The amount of 7-5180 consumed by the animals on a mg/kg of body weight/day basis was proportionalto the dose as follows: Overall Mean SD 1 0.06 0.013 T-5180 (mg/kq/day) Diet Concentration (ppm) 10 30 0.64 0.138 1.94 0.408 100 6.50 1.214 22 HWI 6329-100 Hormone Analysis Hormone analysis data are summarized in Table 34; individualdata are in AppendixE. There were no statisticallysignificantdifferencesbetweengroups. There was no consistentpattern for the hormone determinationswhich could be attributedto the test materialover the entirecourseof the study. None of the hormone levels were significantlydifferentbetween groups although there appeared to be some indicationof elevatedestradiolfor the 100 ppm group at the Week 5 determination. Test Material Residue Analysis Resultsof test materialserum analysisare summarizedin Table 35; individual data are in AppendixF. Serum levelsof T-5180 increasedproportionallywith dietary exposureconcentrationbut there was no increase in serum T-5180 over the course of exposure. Clinical Pathology Hepatic Palmitoyl CoA Oxidase data are summarizedin Tables 6 through 9; individualdata are in AppendixC. The PathologyReport contains a complete discussionof the data. Dietary administrationof the test material,--T-5180c,aused higher hepatic palmitoylCoA oxidase activityat dose levels of 30 and 100 ppm that was statisticallysignificant.The effectwas dose-dependentand reversible. Animals given 10 ppm had transientlyhigher hepaticpalmitoylCoA oxidase activitythat was statisticallysignificantat Week 5 only. The mean enzyme activitiesfor animals given 10, 30, or 100 ppm were highest at Week S. Anatomical Pathology Organ weights, organ-to-bodyweight percentages,and organ-to-brainweight ratios are summarized in Tables 10 through 21; macroscopicand microscopicfindings are summarizedin Tables 22 through 29; incidenceof severityof microscopic observationsare in Tables 30 through33; all individualdata are in AppendixD. The PathologyReport containsa completediscussionof the data. Administrationof the test material,T-5180,caused increasedabsoluteand relativeliver weightsand hepatocellularhypertrophyin the liversof animals afterat least4, 7, and 13 weeks of treatment. Absoluteliver weightsand relativeliver weightsfor animalsfed 100 ppm were significantlyhigherthan the pair-fedcontrolsafter 4, 7, and 13 weeks of treatment. The changes observedare suggestiveof a test materialeffect on intracellularmetabolismand may be 23 HWI 6329-100 associatedwith peroxisomeproliferation.-Duringrecovery,these changes were not observedin animalsthat were treated for at-least13 weeks and untreated for at least 8 weeks, indicatingthat the test material-relatedchangeswere reversible. CONCLUSIONS Based on the resultsof this study, the no-observable-adverseeffect level for T-5180 when fed ad libitumto rats for at least 13 weeks is 100 ppm; the no-effect level is 1.0 ppm. 24 SIGNATURES Jap(i,4eD. Maneke Date StWy Coordinator Toxicology Matthew J. PalA@z-i'olot- Date Diplomate, AB*K @5( Study Director Director Toxicology by and for Hazleton Wisconsin, Inc. HWI 6329-100 25 HWI 6329-100 REFERENCES Games, P. A., and Howell,J. F., "PairwiseMultipleComparisonProcedureswith Unequal N's and/or Variances: A Monte Carlo Study,"Journal of Educational Statistics,1(2):113-125(1976). Instituteof LaboratoryAnimal ResourcesCommissionon Life Sciences, Guide for the Care and Use of LaboratoryAnimals,NIH PublicationNo. 86-23, U.S. Department of Health and Human Services,PublicHealth ServiceNational Institutesof Health: Bethesda,Maryland (1985). Levene,H., "RobustTests for Equalityof Variances," Contributionsto Probabilityand Statistis.1(,eds.)1. Olkinet al., Ch. 25, pp. 278-292,Stanford UniversityPress: Stanford,California(1960). Winer, B. J., "Designand Analysisof Single-FactorExperiments," Statistical Principlesin ExperimentalDesign, Second Ed., Ch. 3, pp. 149-260,McGraw-Hill: New York, New York (1971). 26 HWI 6329-100 PATHOLOGY REPORT SUMMARY Dietary administrationof T-5180 caused higher hepatic palmitoyl CoA oxidase activityat dose levelsof 30 and 100 ppm that was statisticallysignificant. The effect was dose-dependentand reversible. Animals given 10 ppm had transiently higherhepaticpalmitoylCoA oxidaseactivitythat was statisticallysignificant at Week 5. The mean enzyme activitiesfor animalsgiven 10, 30, or 100 ppm were highest at Week 8. Administrationof the test material,also caused increasedabsolute and relative liverweightsand hepatocellularhypertrophyin the liversof animalsafter at least 4, 7, and 13 weeks of treatment. The changes observed are suggestiveof a test materialeffect on intracellularmetabolismand may be associatedwith peroxisomeproliferation. In the recoveryphase, these changes were not observed in animalsthat were treated for at least 13 weeks and untreatedfor at least 8 weeks, indicatingthat the test material-relatedchangeswere reversible. INTRODUCTION The purposesof this study were to assess the subchronictoxicityof the test material,T-5180 (AmmoniumPerfluorooetanoate)w,hen fed to rats for at least 13 weeks and to evaluatereversibilityof any toxic effects. Four groups of male Crl:CDOBRrats (55/group)were fed diets containingthe test materialat levelsof 1, 10, 30, or 100 ppm. Two controlgroups receivedno test material;one control group was pair-fedwith the high-dosegroup throughouttreatment. Ten animals were designatedas recovery animals from each group except for the pair-fed controls. After at least 13 weeks of treatment,these animalswere observedfor reversibility,persistence,or delayedoccurrenceof toxic effectsfor at least 8 weeks posttreatment. During Weeks 5, 8, 14, and 22, animalswere sacrificedand necropsied;macroscopic observationswere recorded,organ weights were obtained, and tissues were preservedin fixativesas requiredby the protocol. One animal,sacrificeddue to poor healthduring Week 4, was also necropsied. At the scheduled sacrifices, serum samples were collected for hormone analysis, tissue samples were collected and frozenfor possibleanalysisfor test materialresidue,and sectionsof liver were obtainedfor assayinghepaticpalmitoylCoA oxidase activityas an indicator of peroxisomeproliferation.Microscopicexaminationswere performedon lesions, brain,liver,lungs,left testis,and accessorysex organs from all animals. Statisticalcomparisonswere made between the treated groups and the nonpair-fedcontrol group and between the high-dosegroup and the pair-fed control group. 27 HWI 6329-100 RESULTS Clinical Pathology Individualvalues for hepatic palmitoylCoA oxidase activity are in Appendix C. Mean values for each dose group and the results of statistical comparisons are in Tables 6 through 9. Week S. Animals given 10, 30, or 100 ppm had statisticallysignificantlyhigher hepatic palmitoyl CoA oxidase activity than the nonpair-fed control animals. Animals given 100 ppm also had statisticallysignificantlyhigher enzyme activity than the pair-fed control animals. Enzyme activity increased as dose levels increased. Week . Animals given 30 or 100 ppm had statisticallysignificantlyhigher hepatic palmitoyl CoA oxidase activity than the nonpair-fed control animals. Animals given 10 ppm had an apparently higher enzyme activity than the nonpair-fed control animals,but the differencewas not statisticallysignificant. Animals given 100 ppm also had statisticallysignificantlyhigher enzyme activity than the pair-fed control animals. Enzyme activity increased as dose levels increased, and the activitiesfor animals given 10, 30, or 100 ppm were higher at this interval than at Week 5. Week 14. Animals given 30 or 100 ppm,had statisticallysignificantlyhigher hepatic palmitoyl CoA oxidase activity than the nonpair-fed control animals. Animals given 100 ppm also had statisticallysignificantlyhigher enzyme activity than the pair-fed control animals. Enzyme activity for animals given 30 or 100 ppm were lower at this interval than at Weeks 5 or 8. Week 22. There were no differences for hepatic palmitoyl CoA oxidase activity between the nonpair-fed control and treated groups. Anatomical Patholoov Individual anatomicalpathology data are in Appendix D. Individual terminal body weights, absolute organ weights, organ-to-body weight percentages, and organ-tobrain weight ratios are also in Appendix D. Mean weight values for each group are in Tables 10 through 21. Incidence summaries of the macroscopic and microscopic findings are in Tables 22 through 29. Scheduled Sacrifices. After 4 weeks of treatment, animals given 100 ppm had significantly lower terminal body weights when compared with nonpair-fed control animals. After 4, 7, or 13 weeks of treatment,absolute and relative liver weights were significantly higher for animals given 100 ppm when compared with those of either group of control animals. These liver weights were also significantlyhigher in animals given 30 ppm after 4 or 7 weeks of treatment. Liver-to-bodyweight percentageswere significantlyhigher in the animals given 10 ppm after 4 weeks of treatment and in the animals given 30 ppm after 13 weeks of treatment. After 13 weeks of treatment, other significant organ weight changes included lower liver-to-bodyweight percentages in animals given 1 ppm and higher testes-to-bodyweight percentages (when compared with those of the nonpair-fedcontrols) in animals given 100 ppm. 28 HWI 6329-100 Macroscopically,there were few observationsand no major differences in incidence betweencontroland treatedanimalsafter 4, 7, or 13 weeks of treatment. Microscopically,minimal-to-slighthepatocellularhypertrophywas observed in the liver from animalsgiven 10, 30, or 100 ppm after 4, 7, or 13 weeks of treatment. This change was characterizedby an accentuatedcentrilobularpattern in which the hepatocytes appeared to have a more homogeneous cytoplasm, and the cell borders were rounded giving the cells a more plump appearance. Except for the enlarged appearance,the cells were otherwiseunremarkable. The incidenceand severityof this change increasedwith dose, but neitherappearedto be affectedby the length of treatment(Tables30 through33). All other observationswere considered incidentalfindings. RecoverySacrifice. Animals given I ppm had significantlyhigher absolutebrain weights. There were no other significantchanges in the remainingorgan weight data. Macroscopicallyand microscopically,there were few observationsand no major differencesin incidencebetween control and treated animals after 13 weeks of treatmentand after 8 weeks without treatment. DISCUSSION Dietaryadministrationof the test materialcausedhigher hepaticpalmitoylCoA oxidaseactivityat dose levelsof 30 or 100 ppm that was statistically significant. The effect was dose-dependentand reversible. Animals given 10 ppm had transientlyhigher hepaticpalmitoylCoA oxidaseactivitythat was statisticallysignificantat Week S. The mean enzyme activitiesfor animalsgiven 10, 30, or 100 ppm were highest at Week S. Elevationof hepatic palmitoylCoA oxidaseactivityindicatesthat the test materialcauses hepatocellularperoxisome proliferation. Dietaryadministrationof the test materialcaused an increasein absoluteand relativeliverweightsafterat least4, 7, or 13 weeks of treatment. Animals treatedfor at least 13 weeks and untreatedfor at least 8 weeks had no significantchangesin liverweights. Microscopicallym,inimal-to-slight hepatocellularhypertrophywas observedin the liversfrom treated animals. These liver sectionshad an accentuatedcentrilobulatedpattern in which the hepatocytes around the central vein appearedto be more plump and larger. In comparison with similarcells from the controlanimals,the cytoplasmof the affectedcells appearedto be more homogeneousand lacking intracellularvacuoles. The cell borderswere more distinct and the cells appearedto be more rounded. There was no evidence of any degenerativechanges or abnormalitiesassociatedwith the hypertrophy. The severityof the cellularenlargementdid not appear to increase with length of treatment,and the absenceof similarchanges in the recovery animalsis indicativeof reversibility.The changesobservedin the liver are possiblyfrom peroxisomeproliferationor an increasein intracellularmetabolism, increasedstorageof metabolicproducts,or both. All other organ weight changes and all macroscopicand microscopicobservationswere considered incidental findingsand unrelatedto ingestionof the testmaterial. 29 SIGNATURES HWI 6329-100 eooert L. 'ho Diplomate, American College of Veteirinary Pathologists (Cl niCal Pathology) nv@m4ag'fEr@Psa Pathologist (6329-100.lhm) 081092 7- f Uate Ua e- 30 HWI 6329-100 COMMENTS ON THE DATA Various computers and computer programs were used to analyze data in this study. Because different programs round off or truncate numbers differently, values in some tables (e.g., means, standarddeviations,or individualvalues) may differ slightly from those in other tables, from individualcalculateddata, or from statisticalanalysisdata. Neitherthe integritynor the interpretationof the data was affected by these differences. The test material, T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) is also referencedas FC-143 in the data for test material serum analysis. The "Study Day" for the PATHTOX computer system and the in-life data collection will always be I day different. The PATHTOX system considers the study initiation day as "Day I," but for all in-lifedata collectedmanually or on a data capture system (e.g.,HAZTOX), the study initiationday is "Day 0." The calculation for test material consumption is: Test material constmtion (Dose Level x FoW Consugwtion) 7 x [(Body weight at Start of Week) + (Body Weight Gain for Week + 2)] 31 HWI 6329-100 CODES, ABBREVIATIONS, AND UNITS General Codes and Abbreviations Abbreviationsand Units for ClinicalChemistry Codes for Anatomical Pathology Note: The followinglistsof codes, abbreviations,and units are used by HWI. Some, but not necessarilyall, of this informationmay be needed for this report. 32 NWI 6329-100 N Mean SD; S.D.; STAND DEV A 8 - cv General Codes and Abbreviations Number of measurements in a group. Arithmetic mean. Standard deviation. Groupmean is significantlydifferent from the mean of the nonpair-fed control group (Group 1) at p s 0.05. Groupmean is significantlydifferent from the mean of the pair-fedcontrol group (Group6) at p s 0.05. Not applicable;No value. Number in group. Coefficientof Variance. 33 HWI 6329-100 Abbreviationsand Units'forClinicalChemistry Test Glucose Urea nitrogen Creatinine Total protein Albumin Globulin Albumin/globulinratio Total bilirubin Directbilirubin Indirectbilirubin Cholesterol High-densitylipoproteincholesterol Low-densitylipoproteincholesterol Triglyceride Uric acid Aspartate aminotransferase Alanine aminotransferase Alkaline phosphatase Gamma glutamyl transferase Sorbitol dehydrogenase Bromsulphaleinclearance Lactate dehydrogenase Creatine kinase Amylase Palmitoyl CoA oxidase Calcium Inorganic phosphorus Sodium Potassium Chloride Magnesium Zinc Strontium Total carbon dioxide Bicarbonate Iron Total iron bindingcapacity Unbound iron binding capacity Percentiron saturation Plasma cholinesterase Red blood cell cholinesterase Brain cholinesterase Abbreviation(Units) GLU (MG/DL) UN (MG/DL) CREAT (MG/DL) T PRO (G/DL) ALB (G/DL) GLOB (G/DL) A/G RATIO T BILI (MG/DL) D BILI (MG/DL) I BILI (MG/DL) CHOL (MG/DL) HOL (MG/DL) LDL (MG/DL) TRIG (MG/DL) UA (MG/DL) AST/SGOT (IU/L) ALT/SGPT (IU/L) ALK PHOS (IU/L) GGT (IU/L) SDH (IU/L) BSP (MG/DL) --LDH(IU/L) CK (IU/L) AMYL (IU/L) PCOAO (IU/G) CA (MG/DL) I PHOS (MG/DL) NA (MMOL/L) K (MMOL/L) CL (MMOL/L) MG (MEO/L) ZN (MG/L or PPM) SR (MG/L.or PPM) TC02 (MMOL/L) BICARB (MMOL/L) FE (UG/DL) TIBC (UG/DL) UIBC (UG/DL) FE %SAT (%) CHEP (MU/ML) CHER (MU/ML) CHEB (MU/ML) 34 HWI 6329-100 Abbreviationsand Units-for Clinical Chemistry Test Electrophoresis Albumin Alpha-l-globulin Alpha-2-globulin Beta globulin Gamma globulin Insulin Adrenocorticotropic hormone Cortisol Glucagon Triiodothyronine Thyroxine Electrophoresis High-densitylipoprotein Low-density lipoprotein Very-low-densitylipoprotein Abbreviation(Units) E ALB (G/DL) E A-1 (G/DL) E A-2 (G/DL) E BETA (G/DL) E GAMMA (G/DL) INSULIN (UU/ML) ACTH (PG/ML) CORTISOL (UG/ML) GLUCAGON (PG/ML) T3 (NG/DL) T4 (UG/DL) E-HDL E-LDL E-VLDL 35 Code 1-2 T u m EX NOT TAKEN MISSING UNSUITABLE AUTOLYTIC EXCLUDE BmNIx- n nF nD nm HWI 6329-100 Codes for Anatomical Pathology Definition ANIMAL DEATH CODES InterimsacrificesI and 2 Terminalsacrifice Postrecoverysacrifice#1 Sacrificed moribund MACROSCOPIC CODES Indicatesthat organ is excludedfrom calculations Organ weight not taken; explanationgiven in necropsy notes Organ missing or lost Organ technicallyunsuitablefor weighing Organ autolytic and could not be weighed Weight has been taken,-butwill be excludedfrom all calculations MICROSCOPIC CODES Codes PrefacingNeoplastic Findings Primary, benign neoplasm Primary, malignant neoplasm Metastatic neoplasm Locally invasiveneoplasm Other neoplasm (see coment) Distributionof Findings(n Not specified Focal Diffuse Multifocal Severity Gradel 36 left I 2 3 4 5 HWI 6329-100 Codes for Anatomical Pathology Definition MICROSCOPIC CODES (Continued) Grades for Severityor Amount Minimal - the least amount of change that can be observed with the lightmicroscope Slight - less than averageamount of change, but readily discernible as abnormal Moderate - the averageamount of change that is expected for a lesion Moderately severe (marked)- a marked amount of change with possibleloss of functionof the affectedcells/organs Severe - a great amount of change with probable loss of functionof the affectedcell/organsand frequently involveslarge areas of the organ 37 HWI 6329-100 Table I Summary of Antemortem Observationsa Observation Munched posture Thin Malocclusion Hind legs, not used Crooked,tip of tail Stiff,tail Soft feces Few or no feces Rhinorrhea Epistaxis AloDecia Sores Back Head Neck Bloody crust Back Head Left foreleg Right foreleg Neck Right forepaw Tail Abdomen Squinting Chromodacryorrhea Swollen Both forepaws Both hind paws Left hind paw Right forepaw Tail Necrotic,tail Missing Teeth Tip of tail T-5180 (Dom) 10 30 1LO -L 0 0 0 0 1 0 0 0 0 0 2 0 4 4 a 5 6 2 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 2 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 1 0 0 11 9 5 9 13 1 0 1 1 0 0 0 0 1 2 0 0 0 I 1 1 1 1 0 0 2 1 0 0 0 0 2 2 1 1 0 0 1 0 0 1 0 0 1 0 0 0 0 3 3 2 1 3 0 0 0 0 1 1 .0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 1 0 a This table indicatesthe number of animals for which a conditionwas observedwithoutregardto the specificnature,severity,reversibility, number of incidencesper animal,or lengthof time the conditionpersisted. 38 HWI 6329-100 Table I sumary of Antemortem Observationso Observation Hunched posture Thin Malocclusion Hind legs, not used Crooked,tip of tail Stiff,tail Soft feces Few or no feces Rhinorrhea Epistaxis Alopecia Sores Back Head Neck Bloody crust Back Head Left foreleg Right foreleg Neck Right forepaw Tail Abdomen Squinting Chromodacryorrhea Swollen Both forepaws Both hind paws Left hind paw Right forepaw Tail Necrotic,tail Missing Teeth Tip of tail 7-5180 (ppm) 1 10 30 1LO -2- 0 0 0 0 1 0 0 0 0 0 2 0 4 4 8 5 7 2 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 2 0 0 1 0 0 0 0 0 1 a 0 0 0 1 0 0 0 0 1 1 0 0 11 9 5 9 13 1 0 1 1 0 0 0 1 2 0 0 0 1 1 1 1 0 0 2 1 0 0 0 0 2 2 1 1 0 0 1 0 0 1 0 0 1 0 0 0 0 3 3 2 1 3 0 0 0 0 1 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 1 0 a This tableindicatesthe number of animals for which a conditionwas observedwithoutregardto the specificnature,severity,reversibility, number of incidencesper animal,or lengthof time the conditionpersisted. 39 NWI 6329-100 Table I (@ontinued) Summary of Antemortem Observations' Observation Small movable tissue mass Wart-likelesion Moribund sacrifice Interimsacrifice Terminalsacrifice Recovery sacrifice T-5180 (ppm) -L 10 30 JOA o 0 0 3 0 0 0 0 1 0 0 0 0 0 0 1 0 30 30 30 30 29 30 15 15 is is is 15 10 10 10 10 10 NA a This table indicatesthe number of animals for which a conditionwas observedwithoutregardto the specificnature,severity,reversibility, number of incidencesper animal,or length of time the conditionpersisted. 40 Table 2 MEAN BODY WEIGHTS AND STANDARD DEVIATIONS IG) 13 WK DIETARY TOXICITY STUDY WITH T-5180 GROUP AND DOSE LEVEL IPPNI WEEK: START 1 2 3 4 5 6 7 8 9 10 1 ---------- ------------------------------------------------------------------------------------------------- I .000 2 1.000 3 10.000 4 30.000 MEAN S.D. MEAN S.D. N MEAN S.D. MEAN S.D. N 204.1 1.89 ss 206.4 10.60 ss 202.9 11.18 ss 203.7 10.89 ss 262.5 11.14 ss 264.2 14.13 ss 260.2 IS.30 ss 261.9 14.IS ss 312.9 14.12 ss 313.4 17.52 ss 302.6 24.SO ss 303.6 23.04 55 3SI.6 18.77 ss 3S2.6 20.60 ss 347.4 21.60 ss 344.3 21.70 ss 388.2 20.01 ss 31$.S 23.42 ss 382.6 24.80 ss 320.3 24.64 ss MALES 418.6 22.35 46 411.7 2S.93 43 416.9 27.24 44 401.6 28.72 45 437,7 26.46 40 440.1 26.11 40 438.2 27.$S 40 426.0 29.27 40 457,3 20.60 40 400.9 29.Sg 40 4SI.2 29.71 40 447.6 30.?S 40 475.2 31.11 25 476,9 28.78 2S 479.3 3S.42 2S 468.9 3S.80 2S 493@6 34.00 25 496.3 30.21 2S 497.0 30.04 2S 413.3 31.56 25 SOO.4 Sit 37.Sl 38 25 2S SOO@5 S23 31.89 33 2s 2S Sit I S26 39.37 41 2S 2S 494.4 502 39.43 42 2s 25 ib Table 2 (Continued) MEAN BODY WEIGHTS AND STANDARD DEVIATIONS (6) 13 WK DIETARY TOXICITY STUDY WITH T-5180 GROUP AND DOSE LEVEL IPPM) WEEK: 14 Is 16 17 Is 19 20 21 ---------- ------------------------------------------------------------------------------------------------- I .000 2 1.000 3 10.000 4 30.000 MEAN S.D. N MEAN S.D. N MEAN S.D. n MEAN S.D. N S30.8 44.39 10 S45.9 42.79 10 570.5 SO.64 10 S3$.3 41.SS 10 540.4 43.23 10 SS6.3 44.05 10 580,5 54.24 10 545.3 42.70 10 549.7 47.51 10 560@4 49.45 10 590.2 SS.72 10 556.9 43.51 10 S59.1 46.$l 10 575.4 51.02 10 599.1 S7.49 10 SOO.3 45.10 10 SSO.? 46.S3 10 578.5 52.99 10 597.1 07.60 .10 S?0.4 46.40 10 MALES SOR.2 49.97 10 519.3 53.99 10 808.6 69.08 10 582.5 46.30 10 572.7 S4.30 10 593.7 57.78 to Sil.3 70.13 10 SIS.9 SO.65 10 571.0 SS.22 s 589.8 55.17 5 SOI.5 80.05 5 SIS.2 24.17 5 GROUP AND Dos( Lfvft 5 100.000 6 0.000 TABLE 2 ICONTlIfUED) MEAN NOY WEIGHTS AND STANDARD DEVIATIOMS IG) 13 WK DIETARY TOXICITY STUDY WITH 1-5100 WEEK: MEAN s@o. N KAN S.O. 9 START -----1--------2 -------3------------4-------s--------6 -------7----------8--@ ------- 9 ----t-o-------i-t- 204. e li,so 55 2S3.3' 13.61 55 213.1 10.31 45 255.2 a,36 45 295.4A 17.44 55 295,3 8@54 45 376.4 A 22@04 55 334,8 10.10 45 3S$.4 A 27@32 54 365.2 10.61 4s NALES 387,SA 32.04 44 410.9' 37.09 40 396.3 13.69 30 416,7 is.a4 30 432 .2A 39.23 40 434.5 19.28 30 439. 1A 46,33 25 441.8 18.06 Is 454.3A 49.71 25 464@3 18.39 Is SA 461. 58.70 2s 470@o 18.67 Is 473 .2A 57,61 ?5 482.3 19.26 Is GROUP AND DOSE LEVEL (PPN) ----------- 5 100.000 6 0.000 TABLE 2 (CONTINUED) MEAN WDY WEIGHTS AND STANDARD DEVIATIONS (G) 13 WK DIETARY TOXICITY STUDY WITH T-5180 WEEK: 14 is 16 17 Is 19 20 21 ---------------------------------------------------------------------------------------------------------------------- MEAN S.D. N MEAN S.D. N 506.2 42.93 10 - 516.6 43.83 10 - 531.6 51.67 10 - 536.7 48.10 10 - 542.0 48.84 10 - MALES 550.4 51.43 10 - 548.9 72.78 10 - 523.3 77.74 5 - 13 WK Table 3 MEAN BODY WEIGHT GAIN (0 DIETARY TOXICITY STUDY WITH T-5180 GROUP AND DOSE LEVEL IPPMI WEEK 0-1 0-2 0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-11 0 ------------------------------------------------------------------------------------------------------ MALES I .000 2 1.000 3 10.000 4 30.000 MEAN S.D. N MEAN s@o. N HEAR S.D. N MEAN S.D. N 58.3 108.7 147.4 184.0 21S.0 233.9 253.5 272.5 291.0 303.7 31S.1 32 4.S9 9.3S 12.70 16.76 18.16 21.67 23.80 24.04 26.?S 30.21 31.Sg 3 55 55 55 ss 46 40 40 25 25 2S 2S 2 57.8 5.53 55 107.1 9.75 ss 146.2 13.Sg 55 182.2 16.84 55 212.7 20.S7 43 234.0 22.16 40 254.8 2S.69 40 271.4 24.01 25 290.8 ZS.60 2S 304.0 27.31 2S 318.0 32 29.44 3 2S 2 S7.3 5.17 55 A 90.7 144.4 179.6 212.1 234.0 254.1 276.0 294.5 301.5 322.7 33 21.16 14.31 13.20 21.18 22.40 24.54 30.S4 34.01 34.20 36.43 3 55 55 55 44 40 40 25 25 25 2S 2 58.2 100.0 A 142.6 176.7 203.2 222.0 243.0 204.8 279.2 290.3 305.3 31 4.93 19.32 14.80 20.67 21.92 23.92 2S.69 30.S2 33.97 34.86 34.19 4 ss ss ss ss 4S 40 40 25 2S 25 2S 2 Table 3 (Continued) MEAN BODY WEIGHT GAIN 10 13 WK DIETARY TOXICITY STWY WITH T-SLIO GROUP AND DOSE LEVEL PP14) WEEK 0-15 0-16 0-17 0-18 0-19 0-20 0-21 ------------------------------------------------------------------------------------------------------ I .000 2 1.000 3 10.000 4 30.000 P4ALES MEAN 340.4 349.8 350.1 3SO.7 301.2 372.7 376.1 S.D. 36.39 41.08 40.08 40.06 43.89 48.33 SO.30 N 10 to to 10 10 10 5 MEAN S.D. 3SI.9 42.15 to 302.1 47.90 to 371.0 SO.01 10 374.2 SI.42 10 3$S.0 S3.09 to 389.4 S7.21 10 384.1 S3.09 s MEAN S.D. N 379.1 $3.20 10 311.7 55.43 10 390.4 S7.50 10 390.4 61.12 10 407.2 19.24 10 410.0 70.33 10 393.3 77.07 s MEAN S.D. N 334.7 38,0$ to 348.4 39.21 to 357.8 41.10 10 361.8 42.21 10 374.0 42.68 to 3$0.3 48.Sl 10 379.3 29.2S 5 TAOTE 3 (CONTINIMB) MEAN BODY WEIGHT GAIN IG) 13 WK DIETARY TOXICITY STUDY WITH 1.5180 GROUP AND DOSE LEVEL ------ 5 100.000 6 0.000 Y@EK: ......0.-.1......0-2------0---3------0--4------0---5-----0---6-------0--1-----0---8------0---9--------0-----1--0------------0--I1-1-----0---1-2----- MEAN 5 D. N(AN s 0. 41.op 15-05 55 42.0 5.16 4s ag.OA 18.31 55 84.4 11.34 45 123 gA 17.41 55 121.7 8.73 4S 153 gA 23@62 S4 IS2.0 8 Si 45 le?.?A 26.43 44 182.1 10.13 45 MALES 2D6.2 A 30.84 40 227. e 32.96 40 202.5 12-79 30 219.6 16.55 30 234@9' 38.10 25 235 2 16.45 Is 250.() A 41.43 25 251.8 16.06 Is 25y.2A 50-62 25 257.4 16.60 Is 269 OA 49.96 25 269 8 17.07 Is 219 0 53.9 25 773.7 24.9 Is jib -4 GROUP AND DOSE IEV(T (ppm) 100,000 WEEK: MEAN s0 N TABLE 3 "IAN eoy WEIGUR GAIN (Gi 13 W 0 IIIARY 1()j(ICIISV,Uor WilliY_Slao ----------------0---l--a------ 0-ig 0-?o 0-21 313 8 10 329.0 331-6 S2.@3 10 48 28 10' 3@36. 9 345 3 48.94 10 51,39 la "Atis 343.388 73. 10 313.() 76-57 5 --------------------------------- GROUP AND DOSE LEVEL (PPM) ----- Table 4 MEAN FOOD CONSUMPTION AND STANDARD DEVIATIONS 13 WK DIETARY TOXICITY STUDY WITH T-SIIO (G/WK) WEEK: 1 2 3 4 5 a 7 1 9 10 11 ---- ---------------------------------------------------------------------------------- I .000 2 1.000 3 10.000 4 30.000 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 108.6 10.SS 54 167.2 10.95 S4 ISO.S 12.18 53 108.6 10.67 54 180.7 13.04 53 178.3 13.44 ss 179.2 19.78 S3 183.3 24.70 54 110.1 ll.OS S3 108.2 14.4S 52 183.8 17.2S so 178.7 33.72 52 193.8 14.30 51 196.0 15.10 S2 191.0 16.74 49 190.2 17.S$ 52 IOS.S 14.32 43 201.4 14.87 38 199.0, 14. 2 40 203.0 20.77 39 MALES 195.3 14.71 3s 117.0 14.30 33 194.4 14.63 37 193.0 18.11 37 192.9 14.83 38 195.4 15.81 36 194.4 14.39 39 193.0 15.69 37 lll.5 17.98 24 191.5 13.13 24 189.0 14.22 23 188.8 IS.53 23 193.1 16.43 24 196.8 12.20 23 193.1 10.41 24 191.9 15.81 24 119.5 16.18 24 190.4 12.41 24 190.9 IS.21 23 187.3 14.15 22 114.7 20.01 23 190.2 15.77 23 189.2 IS.64 24 Its.$ 14.91 24 to GROUP AND DOSE LEVEL IPPM) ----- I .000 2 1.000 3 10.000 4 30.000 Table4 (Continued) MEAN FOOD CONSUMPTION AND STANDARD DEVIATIOGS 13 WK DIETARY TOXICITY STUDY WITH T-518 IG/WK) WEEK: 15 is 17 is 19 20 21 ----------------------------------------------------------------------------------------------- MEAN S.D. N MEAN S.D. N MEAN S.D. MEAN S.D. N 180.2 11.56 10 171,7 19.60 10 115.9 17-16 10 177.1 9.35 10 174.8 13.67 10 177.3 17-29 10 113.0 IS-82 to 181.2 12.37 a 179.2 0.50 10 176.1 21.40 10 183.9 10-48 10 113.e 9.3s 9 191.4 IS.71 9 191.1 17.28 10 116.3 30.04 9 loo.1 I.ss 9 112.4 20,4S 10 187.2 19.12 10 189.1 21.95 10 193.9 8.32 9 MALES 181.1 19.40 10 112.1 20-56 10 189.6 19.12 10 119.1 9.92 0 190.5 11.90 s les.0 25.32 5 181.1 lt.so s 192.0 ii.se 5 CD TABLE 4 (CONJINU(D) WAN rooo co"sumpiion 16/wK) 13 WK DIETARY JUXICITY SlUoy WITH 1-5180 GROUP AND ()OSFttvtt ------ 5 100.000 6 0.000 WE-E-K:-------1--------2 -----.-.-.3-...----4--------5-------6--------7 ----------------9---------------10--------------------- NAT[S MEAN 155 OA 16S.1 A 179.3 185.3 193.1 190.3 187.7 181.6 185 2 176.5 so 9.54 11 36 15 59 15.76 17.26 17,91 17.56 18.48 19.18 172.9 17 26.25 26.56 N 55 55 55 54 47 39 37 29 25 24 24 2 2 MFAN 153.8 164.8 178.0 183.0 187.9 183.5 176.6 116.3 119.5 171.4 170.0 1? so 2 13 0.74 4 57 4.23 10.17 11.00 12.30 9.11 8.43 6.05 4.74 N 4s 44 43 43 23 25 30 14 Is 12 14 1 GROW AND DOSE LEVEL IPPM) ----------- 100.000 TABLE 4 (CONTINUED) KAN FOOD CONStMpTloN (6/WK) 13 WK DIETARY TOXICITY STUDY WIT" T-5180 WEEK: 14 is 16 17 Is 19 20 21 --------------------------------------------------------------------------------------------------------------------- MALES MEAN 164.8 178.0 176.0 1?2.6 178.8 177.6 164.5 174.9 SD 22.S2 19.02 22.12 18.40 11.16 16.15 41.88 15.95 N 10 10 10 10 10 10 10 10 GROUP AND DOSE LEVEL (PPMI ----- I .000 2 1.000 3 10,000 4 30.000 Table 5 MEAN COMPOUND CONSUMPTION 13 WK DIETARY TOXICITY STUDY IMG/KG/DAY) WITH T-5110 W-E-EK-:-----------------2---------3---------4 --------5---------a --------7---------a--------9----------- MEAN S.D. N MEAN S D. N MEAN s D. N MEAN S.D. N .00 .00 54 .09 .00 54 .91 .04 53 2.75 .10 54 .00 .00 53 .01 .00 ss .15 .13 53 2.S9 .33 54 .00 .00 53 .08 .00 52 .7S .05 so 2.20 .39 52 .00 .00 51 .07 .00 52 .71 .03 49 2.14 .15 52 MALES .00 .00 43 .07 .00 38 .61 .03 40 2.13 .15 39 .00 .00 35 .06 .00 33 .03 .03 37 1.96 .13 37 .00 .00 38 .00 36 .60 .02 39 i.es .08 3? .00 oo 24 .06 .00 24 56 .02 23 1.72 .07 23 .00 .00 24 .06 23 .56 .03 24 1.71 .08 24 GROUP AND DOSE LEVEL (PPNI ---------- WEEK: 13 ------------------ I 000 2 1.000 3 10.000 4 30.000 MEAN S.D. N MEAN s 0. N MEAN S.D. N MEAN S.D. N .00 .00 20 .05 .00 22 .47 .03 23 1.44 .10 23 Table 5 (Continued) MEAN COMPOUND CONSUMPTION (MG/KG/DAY) 13 WK DIETARY TOXICITY STUDY WITH T-5180 ab s (CONIINUED) KAN torm CDNSLWIION INGIKGII)AII vil" 13 YK DitiARY1011ti'VS'WV GROUP AND goSE LEVEL ...... 100.000 0.000 I-A-EK---.....I........-.-------------!---------- ---s-----@----!.... VALES KAN S.O. N KAN S.D. 0 6.75 0.34 55 .00 @oo 45 0.35 55 '00 '00 45 0,43 55 .00 .00 45 7,39 0.40 54 .00 00 45' 7.13 0.36 42 @oo 00 30' 6,64 0,36 39 .00 @oo 30 6.21 0.32 37 .00 .00 30 .......-.-----i-o-------!-!......!.@ -.------ s@as 0.35 25 @oo 00 IS* s@64 0,39 25 '00 .00 Is 5@46 0,47 24 .00 '00 5@23 0,46 24 .00 @oo 5@ts 0.41 25 .00 .00 Is tn Table 6 SUMMARY OF CLINICAL CHEMISTRY MALES WEEK 5 DATA DOSE PPM ------ PCOAO IU/G -------- 0 MEAN a S.D. s N 5 MEAN S.D. N 10 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 0 MEAN S.D. N 9 1.7 s A 14 3.6 s 24 11.4 s AS 37 14.6 5 8 .4 c,n Table 7 SUMMARY OF CLINICAL CHIMISTRY MALES WEEK I DATA DOSE PPM ------ 0 MEAN S.D. PCOAO IU/0 -------- 7 I.S MEAN S.D. N 10 MEAN S.D. N 30 MIEAN S.D. N 100 WAN S.D. 0 WAN S.D. N 7 s Is S.S s A 32 12.2 .-S As 54 3S.3 s 7 I.S s tn Table 8 SUMMARY OF CLINICAL CHEMISTRY MALES WEEK 14 DATA DOSE PPM ------ 0 MEAN S.D. PCOAO TU/0 -------- 6 .9 MEAN S.D. N 10 MEAN s.D. 30 WAN S.D. N 100 MEAN s.D. N 0 MEAN S.D. N I 1.9 5 10 2.1 5 A 14 3.4 5 AB I? 4.S s 5 1.1 5 Table 9 SUNWRY OF CLINICAL CHEMISTRY MALES WEEK 22 DATA DOSE PPM ------ PCOAO JU/0 -------- 0 MEAN 7 s 0, .7 MEAN 7 S.D. 1.6 N 5 10 MEAN I S,D. 1.1 30 MEAN 7 S.D. 1.1 N s 100 MEAN 7 S.D. N 5 tn %a HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. TABLE 10 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA 10) 5 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLTIDES: SEX-ALL; OROUP-ALL;WEEKS-ALL DEATH-I;SUBSET-ALL SEX: ------------------------M-AL-E---------------------------- GROUP: ---I--- ---2 --- ---3 --- ---4 --- ---s --- ---a --- HUMBER: is Is Is Is 14 is ---------------------------------------------------------------------------------------------------------------- ))) TERMINAL BODY WEIGHT 191 9 IN GRP.: Is Is Is is 1"4"A Is N E A N : 411.1 413.9 398.4 403.3 378.4 381.9 STAND DEV: 21.7. 34.S 32.0 35.S 18.4 11.1 --------------------------------------------------------------------------------------------------------------- ))) OR - BRAIN (4( I IN GRP.: is Is is is 14 Is M EA N : 2.0732 2.0275 2.0452 2.0604 2.0437' 2.0172 S-T-AN-D--D-E-V-: ------.-0-8-1-2 -----.-0-8-42------.-09-6-6------.-07-5-9-----.-0-9-9-0------O-IS-7 :1-------------------M---------------------------- @)) Ll - LIVER ((( I IN GRP.: is is Is ISA 14AB Is H E A N : 16.3408 IS.4495 17.1942 23.2303 25.4377 IS.1344 S-T-A-ND--D-E-V-:-----2-.-1-3-78-----1-.713-9-----2-.1-2-9-4----2-.-8-3-82-----1-.-9-9-00-----1-.-1-3M0--S--W---w -------w ----------------------------- LU - LUNG ((( I IN GRP.: is Is is Is 14 Is H EA N : 1.5138 1.5312 1.4426 1.4932 1.4140 1.5376 STAND DEV: .2006 .0989 .1122 .1231 .0828 .1268 ------------------------------------------------------w---------------------------------------------------- >)) TEO - LF. TESTIS ((( I IN GRP.: Is is is is 14 Is M EA N : 1.6442 1.6222 1.6454 1.5174 1.5794 1.6102 STAND DEV: .12SO .1410 .1241 .0916 .1704 .1234 ---------------------------------------------------w ---------------------------------------------------- MAZLETO" LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. TABLE 10 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA IG) 5 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKSoALL DEATH-I;SUBSET-ALL SEX: ------------------------1-4A-LE---------------------------- GROUP: ---I--- ---2 --- ---3 --- ---4--- ' ---S--- ---$--- NUMBER: Is Is Is Is 14 is --------------------------------------------------------------------------------------------------------------- TEI - NT. TESTIS (tc 0 IN GRP.: 15 Is Is Is 14 Is H EA N : 1.0523' 1.6168 1.6520 1,5804 1.6260 1.6001 STAND DEV: .1273 .1243 loss .1085 .1013 .1212 ------------------------------------------------------------------------------------------------------------- ))@ SO - ACCESS/SEX ORO (<< I IN GNP.: Is Is Is is 14 is M EA N : 2.0801 2.6799 2.13SO 2.6973 2.0319 2.4898 STAND DIV: .2257 .3S92 .4086 .3136 .2S4$ .3579 ---------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. TABLE II SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (Gi I WEEK SACRIFICE ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATI#-2;SUBSET-ALL SEX: ------------------------M-AL-E- --------------------------- GROUP: ---I--- ---2--- ---3 --- ---4 --- ---5 --- ---a --- NUMBER: is Is Is is Is Is ---------------------------------------------------------------------------------------------------------- ))y TERMINAL BODY WEIGHT (91 I IN GRP.: Is is is Is 1i1s, Is M E A N : 473LI @404.0 470.9 460.0 450.0 440@3 STAND DEV: 20.0 30.0 21.6 2$.? 34.2 24.0 ------------------------------------------------------------------------------------------------------ 0 In GRP.: M EA N : Is 2.0923 ))) OR - BRAIN <<( Is 2.0739 LS 2.0152 t5 2.1032 15 Is 2,0855 - 2.0862 STAND DEV: .0696 .0649 .01st .0050 .0101 .0907 --------------------------------------------------------------------------------------------------------------- ))) Ll - LIVER ((( 0 IN GRP.: Is Is Is ISA ISAS Is M E A N : 17.7610 17.6791 12.4los 24.0114 27.?SS? 16.90?1 STAND DEV: 2.1244 2.7374 2.0972 3.5798 3.507? 2.2232 ------------------------------------------------------------------------------------------------------------- )@) LU - LUNG ((( I IN GAP.: is is Is is Is is NEAN : 1.5129 1.6178 I.S339 1,4902 1.5201 1.50is STAND DEV: .1077 .1110 .1297 .1217 .1904 .1317 ----------------------------------------------------------------------------------------------------------------- I IN GRP.: HEAN @ - S-T-A-ND---D-E-V:---- ))@ TEO - Lf. TESTIS ((( is is is Is 15 Is 1.6801 1.6865 1.6664 1.7163 1.6865 1.6305 .1110 .0821 .2296 .1649 .1265 .1177 ------------------------------------------------------------------------------------------------ cn TABLE It SUMt4AIy OF ABSOLIJYE ORGA" WE'(I#ilDATA 161 WEEK SACITIFICE tiAZLEYON LL%BORATORIES AVXITIC-A. MADISON- W'SCo"Slt4 U.S.A. ------------------------------------- ------------------------- ------------------------------------------------- TASLE,,AINCLUDES@ :WEEKS-ALL SE *ALL.01toUP-ALL ------------ TH-2:SUBSEYeALL DE -..--14ALE---------------- -.-e--- -------------- 4 --- ---5 3 --- --- Is -----I-s ------- SEX! :::- 2 --- --I---------I-s--------------- altoup t&U"BER: -----i-s--------is-- Ity. JESTI C(t 15 14 ---------- ;;;-T-E-I---is is I-GS33 1.633S -------------- fj 114 GNP Is 5 . I1 .6875 t@ 69 4 1.7911 .1070 -'-L-i--l-t----------------------- .2401 -------------- t4 E A " 1.7030 .1076 @io 52 ...... .13 -1----------- S-T-A-ND---DE-V-:------------- ----------- S/ EX Ra ))) SO - Is is s I2.1206 Is Is I 3. 444$ 3@1306 2-.I2S?1I3S -----.-4-39-1------------------------ IN GNP. M E A MV: 3loGI3 3.0290 .4021 3640 .3124 ------- :-T-A-r-iD-D-E--:-------.3-6-1-7--------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. TABLE 12 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (G) L4 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES@ SEX*ALL;GROUPeALL;WEEKS-ALL I)EAtHsl-,SUBSET-ALL SEX: ------------------------M-A-LE----------------------------- GROUP: ---I--- ---2 --- ---3 --- ---4 --- ---5 --- ---a --- ---H-UM-B-ER-:-------Is--------I-s--------I-s--------I-s--------is--------I-s-------------------------------- )@) TERMINAL BODY WE143HT 191 (<f 6 IN GRP.: is 14 Is Is 14 is M E A ti : ssi.? SSS.1 552.7 S3$.2 489.1 S03.5 STAND DEV: 41.1 42.0 40.7 56.1 79.0 23.7 --------------------------------------------------------------------------------------------------------------- )@) BR - 13RAIN((( I IN GRP.: Is 14 Is is 14 Is MEAN : 2.1914 2.2279 2.1740 2.1081 2.1342 2.1364 STAND DEV: .1183 .2745 .0996 .0724 .1044 OS94 ------------------------------------------------------7--------------------------------------------------------- ))),Ll - LIVER -((( I IN GRP.: Is 14 Is Is 14 A B Is M E A N : 19,7290 18.03SS 20.4416 22@7367 26.7146 16.3033 STAND DEV: 2.2290 2.8120 2.4657 4.2128 5.4675 1.6241 --------------------------------------------------------------------------------------------------------------- ))) LU - LUNG ((( I IN GRP.: is 14 15 is 14 is NE AN : 1.0543 1.6008 1.5803 1.6101 I.SI23 1.5875 STAND DEV: .1403 .1118 .22SO .1316 .1401 .1018 --------------------------------------------------------------------------------------------------------------- I IN GRP.: M EA N : STAND DEV: ----------- )@) TEO - LF. TESTIS ((4 15 14 Is Is 14 is 1.7591 1.7329 1.7767 1.7771 1.7578 1.7117 .1174 .2279 .1017 '1216 IS47 .1381 ---------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U S.A. TABLE 12 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA ICl 14 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TA13LE INCLUDES: SEX-ALL;GROUP*ALL:WEEKS-ALL DEATH-T;SU13S[T-ALL SEX; ------------------------M-AL-E- --------------------------- GROUP: ---I--- ---2 --- ---3 --- ---4 --- ---5 --- ---6--- ---N-UM-B-ER-:-------is--------i-s-!-----------15--------i-s--------I-s--------I-s------------------------------------------------- ))@ TEI - RT. TESTIS ((4 8 IN GRP.: H EA N : Is 14 1.7572, 1.1269 Is i.isio Is 1.7759 14 1.7373 is 1.6903 STAND DEV: .11.26 .2499 .0712 .1197 .1460 .1204 ----------------------------------------------------------------------------------------------------------- ))) SO - ACCESS/SEX OR(I(<( I IN GRP.: is 14 Is Is 14 Is N EA N : 3.S217 3.4797 3.5435 3,3760 3.3647 - 3.2SO7 STAND DIV: .4974 .4298 .5535 .4782 .6309 .6635 -------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE 13 OF ABSOLUTE ORGAN WEIGHT 22 WEEK SACRIFICE DATA IG) --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL GROUP-1.2.3.4.S;WEEKS-ALL DEAT"-U;S'UBSET-ALL SEX: --------------------MA-L-E ---------------------- GROUP: ---I--- ---2--- ---3 --- ---A --- ---s --- NUMBER: 10 to 10 10 10 --------------------------------------------------------------------------------------------------------------- ))) TERMINAL BODY WEIGHT,@ll ((( I IN ORP@: 10 10 10 10 M E A N : 5?8.1 007.1 629.2 001.1 SSS.0 STAND DEV: 61.6. So.1 73.4 51.2 69.1 --------------------------------------------------------------------------------------------------------------- ))) BR - BRAIN ((( I IN GRP.: 10 IOA 10 to 10 M EA N : 2.1328 2.2465 2.2042 2,1822 2.1345 STAND DEV: .0122 .0496 .0774 .0863 .0909 --------------------------------------------------------------------------------------------------------------- Ll - LIVER I in GRP.: to to 10 10 10 M I A N : 18.6432 11.43?3 18.9203 20.7334 11-6190 STAND DEV: 3@0867 1.7410 2.2968 1.2916 2.446S --------------------------------------------------------------------------------------------------------------- ))) LU - LUNG 4(( 9 IN GRP.: 10 10 10 10 10 M EA N : 1.5961 1.?4ts l@6800 l@6937 1.5919 STAND DEV: ioso .1548 .1827 .1416 .1130 --------------------------------------------------------------------------------------------------------------- I IN GAP.: M EA N STAND DEV --------- ))) TEO - LF. TESTIS ((( to to to 10 to 1.6934 1.7559 1.7398 1.7055 1.7590 IS36 .1607 .2194 .2246 .1509 ------------------------------------------------------------------------------------------------ HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. TABLE 13 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (Gi 22 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TA13LE INCLUDES: SEX-ALL;GROUP-1.2.3.4.5;WEEKS-ALL DEAFH-U;SUBSET-ALL SEX: --------------------MA-L-E ---------------------- GROUP: ---2--- ---3--- ---4--- ---s --- NUMBER: 10 10 10 10 10 ---------------------------------------------------------------------------------------------------------------- ))) TEI - PT. TESTIS 44( a IN ORP.: to to 10 10 10 M E AN ; 1.7544 ' I.IS92 1.7991 1.7913 1.73ss STAND DEV: .1111 .1739 .1834 lass .1118 ---------------------------------------------------------------------------------------------------------------- ))) SO - ACCESS/SEX ORO 4(4 6 IN GRP.: to to 10 10 to M EA N : 3.5540 3.6582 3.Slgl 3.9577 3.6939 STAND DEV: .5410 .5408 .2931 .4056 .8923 ----------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE 14 OF ORGAN-TO-BODY WEIOHT S WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP&ALL;WEEKSwALL DEATH-I;SUBSET-ALL SEX: GROUP: ------------------------M-AL-E- -----------------------------I--- ---2 --- ---3 --- ---4 --- ---5 --- ---I--- HUMBER: 15 15 Is is 14 is ---------------------------------------------------------------------------------------------------------------- ))) BR - BRAIN c<( 110 GNP. Is is Is is 14 Is H EA N .5053 .4927 SI62 .5142 s4o9 Slll STAND DEV: .0200 .0418 .0474 .0444 .0305 .0207 ---------------------------------------------------------------------------------------------------------------- ))) Ll - LIVER 4(( I IN GNP.: is Is ISA ISA 14A 8 , is N EAN : 3.9080 3.7297 4.4846 S.7612 6.7292 4.0714 STAND DEV: .3714 .2293 .3162 .6020 .4938 .2734 ---------------------------------------------------------------------------------------------------- )@@ LU - LUNG ((t I IN GNP.: Is Is Is Is 14 is H EA N : .3676 .3710 .3625 .3713 .3739 .3#55 STAND DEV: .0413 .0216 .0162 .0271 .0181 .0319 ---------------------------------------------------------------------------------------------------------------- ))) TEO - LF. TESTIS ((( 5 IN GNP.: 15 Is Is is 14 Is H EA N : .4007 .3933 .4142 .3962 .4170 .4130 STAND DEV: .0332 .03SO .0310 .0391 .047S .0288 ---------------------------------------------------------------------------------------------------------------- I IN GRP.: M EA N : STAND DEV: ---------- ))) TEI - RT. TESTIS 4(( Is Is 15 15 14 Is .4025 .3922 .4160 .3944 .4301 .4113 .0309 .0350 .0290 .0414 .0359 .0274 ------------------------------------------------------------------------------------------------ 00 HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. SUMMARY TABLE 14 OF ORGAN-TO-BODY WEIGHT 5 WEEK SACRIFICE PERCENTAGES --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL@GROUPwALL.WEEKS-ALL DEATH-I:SUBSET-ALL SEX: ------------------------0-4A-LE--------------------------- GROUP: ---i--- ---2 --- ---3 --- ---4 --- ---S --- ---0 --- NUMBER: Is Is Is is 14 Is --------------------------------------------------------------------------------------------------------------- ))) SO - ACCESS/SEX ORO 4<9 I IN GNP.: is is 15 15 14 is M EA N : .6530' .6472 .7122 .6710 .6950 .6408 STAND DEV: 'os4s ossl .0010 .0?61 .0570 .0043 --------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE IS Of ORGAN-TO-BODY WEIGHT I WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;OROUP-ALL;WEEKS-ALL DEATH-2;SUBSET*ALL SEX: ------------------------M-AL-E- --------------------------- GROUP: ---I --- ---7 --- ---3 --- ---4 --- ---5 --- ---a --- HUMBER: is is Is is is Is ---------------------------------------------------------------------------------------------------------------- )$) BR - BRAIN ((< I If#GRP. Is Is is is Is is NI A N .4436, .4304 .4445 .4SIg .4ses .4654 STAND DEV: .0311 .0298 .0349 .0274 .0374 .0292 ----------------------------------------------------------------------------------------------------------------- ))@)Ll - LIVER ((< 9 IN GOP.@ Is Is Is ISA ISAB is H EA N : 3.74SI 3.0394 4.1240 S.1384 6.0587 @ 3.7548 STAND DEV: .21SO .3334 .3702 S317 .7182 .37as ---------------------------------------------------------------------------------------------------------------- @),)LU - LUNG 44( 1 110 GAP. H EA N 15 .3352 Is .3355 Is .3251 Is .3198 is .3321 is .3493 STAND DEV: .0249 .0281 .0246 .02?1 .0415 .032S ---------------------------------------------------------------------------------------------------------------- ))) TEO - LF. TESTIS <(( 0 114 GNP. Is Is Is Is Is is MEAN .3561 .3501 .3536 .3612 .3602 .3636 STAND DIV: .0291 .0223 .0429 .0342 .0404 .0279 ---------------------------------------------------------------------------------------------------------------- )),)7.EI - NY. TESTIS ((( I IN GRP.: M E AN : 15 .3609 Is .3SO3 Is .3S22 Is .3851 Is .3620 14 .3641 STAND DEV: .0258 .0323 .0242 .0598 .0342 .0254 ------------------------------------------------------------------------------------------------------------------ HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE OF ORGAN-TO-BODY 15 WEIGHT I WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX*ALL@GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL SEX: ------------------------M-AL-E- --------------------------- GROUP: ---t--- ---2 --- ---3 --- ---4--- ---S--- ---6--- NUMBER: is Is Is Is is is ---------------------------------------------------------------------------------------------------------------- ))@ SO - ACCESS/SEX ORO (44 4 IN GNP.: is Is Is Is Is Is 14 E A N : .6471 .0419 .7340 .8720 .06125 .0439 STAND DEV@ OSS? .0139 .0000 @072S 62 .1020 ---------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE 16 OF ORGAN-TO-BODY WEIGHT 14 WEEK SACRIFICE PERCENTAGES --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;(IROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL SEX; ------------------------M-AL-E- --------------------------- GROUP: ---I--- ---2-.- ---3--- ---4--- ---s--- NUMBER: is Is Is Is is is --------------------------------------------------------------------------------------------------------------- ))) SR - BRAIN <(( I IN GRP.: 15 14 is 15 14 Is N E AN : @3944, .4037 .3951 .3954 .4468 .4250 STAND DEV: .0377 .0613 .0296 .0401 .0712 .0111 ---------------------------------------------------------------------------------------------------------------- ))) Ll - LIVER ((< I IN GNP.: is 14 A Is ISA 14AB Is MEAN : 3.5293 3.2358 3.8183 4.2106 5.4947. 3.2347 STAND DEV: .2808 @3001 @3238 .5009 .8403 .2274 --------------------------------------------------------------------------------------------------------------- )@) LU - LUNG <(( I IN GNP.: H EA N @ is .2961 14 .2192 Is .2156 Is .3016 14 .3130 Is @31SO STAND DEV: .0239 .0329 .0329 .0348 .0351 .0247 --------------------------------------------------------------------------------------------------------------- ))@ TEO - LF. TESTIS ((( 9 IN GRP.: is L4 Is Is 14 Is MEAN : .3182 .3124 .3234 .3327 .3653 .3400 STAND DEV: .0292 .0366 .0343 .0322 .0416 0224 --------------------------------------------------------------------------------------------------------------- ))) TEL - RT. TESTIS <(( 0 IN GRP.: Is 14 Is Is 14 A Is M E AN : .3160 @329S .3189 .3610 .3360 SIAND DEV: .0296 .0392 .0305 .0464 .0216 --------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE Of ORGAN-TO-BODY 16 WEIGHT 14 WEEK SACRIFICE PERCENTAGES ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL GROUP-ALL;WEEKS-ALL DEATH-T;S'UBSET-ALL SEX: ------------------------MA-L-E---------------------------- GROUP: ---I--- ---2 --- ---3--- ---4 --- ---5 --- ...6 --- HUMBER: Is is is is Is is ----------------------------------------------------------------------------------------------------------------- ))) SO - ACCESS/SEX ORO tt( I IN GRP.: Is 14 Is Is 14 15 MEAN : .6327 .8273 .8454 .0381 .0910 .6470 STAND DEV: .0037 .0641 .1221 .1240 .0940 .1241 ---------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE 17 OF OOtGAN-70-BODY WIT(KHT PERCENTAGES 22 WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE 114CLUDES: SEX-ALL;GROUPOALL;WEEKS-ALL DEA7"-U;SUBSET-ALL SEX: ------------------------M-AL-E- ----------------- GROUP: ---I--- ---2 --- ---3 --- ---4 --- ---5 --- ---H-UM-B-ER-:-------10--------1-0-7 ----------1-0--------1-0--------1-0-------------------------------------------------- ),))OR - BRAIN 444 9 1" Opp.: 10 10 10 10 10 M fA N : .3720 .3727 .3S43 .3641 3904 STAND DEV: .0338 .0311 .0397 .0320 .0624 -------------------------------------------------------------------------------------------------------------- )))Ll - LIVER ((( I IN GRP.: 10 10 10 to 10 M E AN : 3.2077 3.0410 3.0169 3.4001 3.304Z STAND DEV: .1976 .1717 .2116 .4271 3s3s -------------------------------------------------------------------------------------------------------------- ))) LU - LUNG 444 I IN ORP.: 10 10 10 10 to M EA N : .2770 .287S .2701 .2821 .2906 STAND DEV: .0187 .0169 .0401 .0214 .0447 -------------------------------------------------------------------------------------------------------------- )@@ TEO - LF. TESTIS ((( a IN GRP.: 10 10 10 10 10 M EA N : .2946 .2904 .2781 .2850 .3210 S-T-AN-D--D-E-V-: -------.0-2-1-1-----.-0-2-66------.-03-4-5------.0-4-3-1------.0-4-9-9---------------------------------------------- 1 IN ORP.: N EA N : - S-@-TA-N-D -D-E-V:--- 3-@)TEI - RT. TESTIS (4( 10 10 10 10 10 .3054 .2912 .2874 .2996 .3187 .03s? .0306 o2s9 .037S .0455 ---------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. SUMMARY TABLE 17 OF ORGAN-TO-BODY WEIGHT 22 WEEK SACRIFICE PERCENTAGES ----------------------------------------------------------------------------------------------------------------- IABLE INCLUDES: SEX-ALL@GROUP-ALL;WEEKS-ALL DEATH-U;SUBSET-ALL SEX: ------------------------M-AL-E- ----------------- GROUP: ---I--- ---2 --- ---3 --- ---A --- ---5--- NUMBER: 10 10 10 10 10 ----------------------------------------------------------------------------------------------------------------- )@) SO - ACCESS/SEX ORO ((( 0 IN OPP@-@ to to 10 10 10 H EA N : .6145, .6057 .5714 .6590 .6792 STAND DEV: .0114 .0989 .0179 OS94 .1950 ----------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. SUMMARY TABLE II OF ORGAN-TO-BRAIN WEIGHT 5 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TA13LE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH*I;SUBSET-ALL SEX: GROUP: ------------------------M-AL-E- -----------------------------I--- ---2 --- ---3--- ---4--- ---s --- NUMBER: Is is Is -------------------------r--- Is ---a--- 14 Is ----------------------------------------------------------------------------------- ))) OR - BRAIN ((,( I In GNP.: Is Is is Is 14 M EA N : 1.0000 1 1.0000 1.0000 1.0000 is STAND DEV: .0000 .0000 .0000 .0000 1.0000 .0000 1.0000 .0000 ----------------------------------------------------------------------------------------------------- )))Ll - LIVER ((( ---------- I IN GRP.: N EA N : Is 7.8802 is 7.6323 Is 1.7711 ISA 11.2764 14A B 15 STAND DEV; .9350 .9170 1.1310 1.3499 12.4713 ' 7.1656 1.0900 .6950 ---------------------------------------------------------------------------------------------------------------- )],)LU - LUNG (<( 8M IEN GARPN.:: Is.7309 is.7504 is.7063 15.7242 14 Is STAND DEV: .0969 .0575 .0573 .04S2 .6925 .0383 .7631 .0659 ---------------------------------------------------------------------------------------------------------------- ))@ TEO - LF. TESTIS ((( I IN GRP.: M EA N : is Is Is is 14 .7232 .8015 .1004 .?713 Is STAND DEV: OS41 .0794 .0739 OS13 .7734 .0852 .1000 .0767 ---------------------------------------------------------------------------------------------------------------- I IN GRP.: M EA N STAND DEV --------- ))) TEI - RT. TESTIS ((( Is .7971 Is .7990 is .1095 is .7679 14 .7971 Is .7951 .0562 .0742 .0644 .0580 .0604 .0771 -------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE It OF ORGAN-TO-BRAIN WEIGHT S WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SIX-ALL;GROUP-ALL;WEEKSmALL DEATH-I;SUBSETPALL SEX: (INOUP: ------------------------M-AL-E------------------------------I--- ---2 --- ---3 --- ---4 --- ---s --- ---0 --- NUMBER: Is Is Is Is 14 Is ----------------------------------------------------------------------------------------------------------------- ),),S)O - ACCESS/SEX ORO ((( I IN OPP.: N EA N : is Is 1.2925 , 1.3224 Is 1.3897 is 1.3098 14 1.2900 is 1.2333 STAND DEV: .0910 .1743 .2163 .1497 .1328 .1670 ------------------------------------------------------------------------------------------------------------ HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE 19 OF ORGAN-TO-BRAIN WEIGHT I WEEK SACRIFICE RATIOS --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SIX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL SEX: ------------------------M-AL-E- --------------------------- GROUP: NUMBER: ---I--is ---2--15 ---3 --15 ---4 --- ---5 --- ---6 --- Is 15 15 ---------------------------------------------------------------------------------------------------------------- ))) OR - BRAIN ((( I IN GRP.: N tA N : 15 1.0000, Is 1.0000 Is 1.0000 is 15 1.0000 Is STAND DEV: .00-00 .0000 .0000 .0000 1.0000 .0000 1.0000 .0000 ----------------------------------------------------------------------------------------------------------- @>@ Ll - LIVER <<( a IN GNP.: HE A N : Is 8.4990 Is 1.5253 15 9.3301 15A 11.4408 ISA 8 15 STAND DIV: 1.0685 ----- 1.2177 1.1031 13.300?8S l.sloo I.Si 1.1170 1.1411 ---- ------------------------------------------------------------------------------------ I IN GRP.: NE A N : Is .7571 )@) LU - LUNG <(( Is Is .7799 .7377 Is .7083 15 .7295 15 .74$S STAND DEV: OS36 .045S .0795 .0518 .0929 .0556 -------------------------------------------------------------------------------------------------------------- I IN GRP.: M EA N : is .8042 @@) TEO - LF. TESTIS ((( Is .8136 is .1004 Is .8172 Is .8111 Is .7831 STAND DEV: .0614 .0395 .1154 .0864 .0722 .0672 ---------------------------------------------------------------------------------------------------------- 9 IN GRP.: Is >)) TEI - PT. TESTIS (4( 15 Is is Is 14 M EAN : ST AND DEV: IIS3 .1140 .8117 .8543 .7939 .7023 -'-------- .0608 osiy .0127 .1342 .0604 .0727 ------------------------------------------------------------------------------------------------ HAZTETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE 19 OF ORGAN-TO-BRAIN WEIGHT I WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2.SUBSET-ALL SEX: GROUP: ------------------------M-AL-E- -----------------------------I --- ---2--- ---3 --- ---4 --- ---5 --- --- NUMBER: is Is Is Is Is a --is ---------------------------------------------------------------------------------------------------------------- ))) SO - ACCESS/SEX ORO ((( I IN GRP.: M EAN : Is 15 1.4672 ' 1.4939 is I.GS24 is 1.4905 Is 1.301S Is 1.3864 STAND DEV: .1780 .1163 .1631 .1662 .1361 .2390 ---------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. SUMMARY TABLE 20 OF OltOAN-TO-BRAIN WEIGHT 14 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL SEX: GROUP: ------I---------------2 --------------3-------M-AL-E------4---------------5---------------a---------- HUMBER: is Is Is Is Is Is ---------------------------------------------------------------------------------------------------------------- @)) ON - BRAIN (c( I It@aftf. Is 14 Is is 14 Is N fA N STAND DEV: 1.0000 , 1.0000 .0000 .0000 1.0000 .0000 1.0000 .0000 1.0000 .0000 1.0000 .0000 ---------------------------------------------------------------------------------------------------------------- ),))Ll - LIVER (<< I IN GRP.: N EA N : Is 9.0327 14 8.1901 Is 9.4115 15 10.7111 14A 0 is 12.6121 - 7.6352 STAND DEV: 1.2007 1.5593 1.3310 1.9614 2.1823 .7740 - --------------------------------------------------------------------------------------------------------- ))) LU - LUNG 4(( I IN GNP.: N EA N : is .7SS7 14 .7259 Is .7264 is .7832 14 .7096 Is .7433 STAND DEV: .0009 .10?S .0982 .0491 .0680 .0482 --------------------------------------------------------------------------------------------------------------- ))) TEO - LF. TESTIS ((( I IN GRP.: H tA N : Is .8053 14 .7844 15 .8191 15 .8430 14 .8255 is STAND DEV: .0723 .1209 .0655 .0491 .0149 .8008 .0544 ----------------------------------------------------------------------------------------------------------------- I IN GRP.: M EA N : - S-T-A-N-D--D-E-V:--- ))) TEI - RT. TESTIS <(( 15 14 15 is 14 Is .1044 .8300 .8074 .8428 .0154 .7908 .0708 .1528 .0532 .0555 .0759 .0441 ------------------------------------------------------------------------------------------------- CD HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. SUMMARY TABLE 20 OF ORGAN-TO-DRAIN WEIGHT 14 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL SEX: ------------------------P-4A-LE---------------------------- GROUP: NUMBER: --- ---I--Is ---2--- ---3 --- Is 15 ---4 --Is ---5 --Is ---0 --Is ---------------------------------------------------------------------------------------------------------- ))> SO - ACCESS/SEX ORG ((( I IN GRP.: M EA N : Is 1.6064 14 1.579S is 1.6211 is 1.6014 14 1.5140 Is 1.5201 STAND DEV: .2047 .2402 .2354 .2191 .3327 .3019 ---------------------------------------------------------------------------------------------------------------- 00 HAZLETON LABORATORIES AMERICA, INC, MADISON, WISCONSIN U.S.A. SUMMARY TABLE 21 OF OftOAN-TO-BRAIN WEIGHT 22 WEEK SACRIFICE RATIOS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX;ALL;GRUOUP-ALL;WEEKS-ALL DEA H-U;S OSET-ALL SEX: ------------------------M-AL-E- ------------------ GROUP: ---I--- ---2 --- ---3 --- ---4 --- ----5 --- NUMBER: 10 10 10 10 10 ----------------------------------------------------------------------------------------------------------------- )@)@)BR - BRAIN c(< 5 IN GRP.: N E AN : 10 10 1.0000 1 1.0000 10 1.0000 10 1.0000 10 1.0000 STAND DEV: .0009 .0000 .0000 .0000 .0000 ----------------------------------------------------------------------------------------------------------------- 0 IN ORP.: N E A tJ : 10 8.7277 1.3187 ))) Ll - LIVER((-( 10 8.2047 10 8.5888 10 9.5071 10 8.7300 STAND DEV- .7112 1.0599 .1021 1.1471 --------------------------------------------------------------- >)) LU - LUNG ((( 7 -------------------------------------------------- 0 IN GNP.: N E AN : 10 10 10 10 .7490 .7751 .7626 10 STAND DEV: .0512 .0645 .0135 .7763 .0618 .74se .0434 ----------------------------------------------------------------------------------------------------------------- I IN OPP.: MEAN : 10 .7052 @)),TEO - LF. TESTIS ((( 10 10 10 .7815 .7903 .7828 10 .8242 STAND DEV: .0799 .0680 .1042 .1097 .0627 ------------------------------------------------------------------------------------------------------------------ I IN GRP.: MEAN : STAND DEV: ---------- 10 ))) TEL - AT. TESTIS ((( 10 to 10 10 .1243 .0966 .7830 .0746 .8101 .0852 .8229 .0917 .1140 .0563 -------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. SUMMARY TABLE 21 OF ORGAN-TO-BRAIN WEIGHT 22 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-U;SUBSET-ALL SEX: GROUP: ------------------------K-AL-E----------------------2--- ---3 --- ---4 --- ---5 --- NU04BER: 10 10 10 10 10 ---------------------------------------------------------------------------------------------------------------- I IN GRP.: 10 >)) SO - ACCESS/SEX ORO ((( 10 10 10 10 ME AN : 1.6634 , 1.6271 1.6320 1.8151 1.7299 STAND DEV: .2255 .2294 .1649 .1973 .3198 ---------------------------------------------------------------------------------------------------------------- co HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INCIDENCE TABLE 22 OF MACROSCOPIC OBSERVATIONS 5 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-I;SUBSET-ALL N UM 0E R 0 F A N IM AL S SEX: -----------M-ALE ------------ GROUP: -I- -2- O-R-G-AN---AN-D--K-E-YW-O-RD-I-S-) ---ON--P-H-R-AS-E--------------------------------N-U-MB-E-R:----I-S----15-- 46 TOP Of LIST ** --- GENERAL COMMENT JOCI ............................N.U.MB.E.R . EXAMINED: IS Is -3Is --Is -4is --Is -514 --14 -0is --Is ALL TISSUES/ORGANS NORMAL 14 13 14 13 11 13 BRAIN IBR) ....................................N.U.M.B.E.R.E.XAMINED: 15 15 IS 15 14 15 NOT REMARKABLE:IS Is Is Is 14 Is LUNG (LU) .......................................N.U.M.B.EREXAMINED: 15 IS 15 15 14 15 NOT RE14ARKABLE: IS 15 Is Is 14 Is LIVER ILI) ....................................N.U.MB.E.R ..E.XAMINED: 15 is is Is 14 is NOT REMARKABLE: 15 Is Is 15 14 15 LF. TESTIS ITEO) CONTAINS FLUID PROSTATE (PRI .... NODULAR .........................N.U.M.B.EREXAMINED: 15 15 15 15 14 15 NOT RE14ARKABLE: 15 Is Is Is 13 15 ........................N.UM.B.ER. EXAMINED: NOT REMARKABLE: 0 0 0 0 1 0 15 Is 15 is 14 15 IS Is Is 15 14 14 0 0 0 0 0 1 co HAZLETON LABORATORIES AP4ERICA. INC. MADISON, WISCONSIN U.S.A. INCIDENCE TABLE 22 OF MACROSCOPIC OBSERVATIONS 5 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;OROUP-ALL;WEEKS-ALL DEATH-I;SUBSET-ALL N UM 0 ER -0 F -A N IM A LS SEX: -----------M-ALE ------------ (IROUP: -I- -2- -3- -4- -5- -6- O-R-G-A-N-A-N-D--K-E-YW-O-R-D-I-S-)--ON--P-H-R-A-SE--------------------------------N-U-M-B-ER-:----I--S-----Is-- is Is 14 15 --- --- --- --- --- SEMINAL VESICLE (SY) ...... NUMBEREXAMINED: IS is 15 is 14 15 NOT REMARKABLE: 15 IS IS IS 14 Is COAGULATING GLD (CGI ............................N.U.M.B.E.REXAMINED: 15 15 IS 15 14 is NOT REMARKABLE: IS 15 15 IS 14 is URETHRA (URI ..................................N.U.M.B.E.R.E.XAMINED: is 15 15 IS 14 Is NOT RE14ARKABLEI:S Is is Is 14 Is SKIN (SKI .....................................N.U.M.B.E.R.E.XAMINED: 15 Is Is Is 14 is NOT REMARKABLE: IS 15 14 IS 14 Is CRUSTED ARIAISI 0 0 1 0 0 0 KIDNEY(S) (KDI .................................N.U.M.B.E.R.EXAMINED: IS 15 is 15 14 is NOT REMARKABLE: 14 13 Is 13 11 14 LARGE PE SENIFLUZOLVI14ASIEERSIAL-PELVIS 1 2 0 2 3 1 0 0 0 0 1 0 SPLIEN (SP) ................... ...............N.UM.BER EXAMINED: 15 15 15 Is 14 is NOT RE04ARKABLE: 15 Is Is 14 14 Is RAISED FOCUS(II/AREAISI 0 0 0 1 0 0 OD HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U-S.A. INCIDENCE TABLE 22 OF MACROSCOPIC OBSERVATIONS 5 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL; OROUP-ALL;WEEKS-ALL DEATH-I;SUBSET-ALL NU H 8E R 0 F A N IMA L S SIEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -A- -5- -6O-R-G-AN---AN-D--K-E-YW-O-RD-I-S-)---O-R -P-H-R-AS-E--------------------------------N-U-MB-E-R:----1-5----I-s- is Is 14 Is --- --- --- --- --- 14ESENTERIC LN (MS) ..............................NU.M.BE.R..EXAMINED: 15 Is Is Is 14 is NOT RE14ARKABLE: IS IS 15 15 14 IS MANDIB/CERV LM (14N) .............................NU.MO.E...EXAMINED: NOT RREMARKABLE: 15 IS IS 15 14 15 15 15 IS Is 14 Is LUMBAR/ILIAC LN ILM) ............................N.UM.B.ER.. EXAMINED: IS IS 15 15 14 Is NOT RE14ARKAOLE : IS 15 Is is 14 Is END OF LIST ccon HAZLETON LABORATORIES AMEPTICA. INC. MADISON, WISCONSIN U.S.A. INCIDENCE TABLE 23 OF MACROSCOPIC OBSERVATIONS I WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL --- N U M 13E R - 0 F - A N I M A L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5ORGAN AND KEYWORDFS) OR PHRASE ;;-T-O-P--O-F--L-I-ST---66---------------------------------------------NU-M-BE-R-: -----1-5-----is is is Is Is GENERAL COMMENT IOCI ............................N.U.MB.E.R . EXAMINED: 15 Is Is Is Is is ALL TISSUES/ORGANS NORMAL MALOCCLUSION BRAIN IBR) ........................................... NUMBER FXAMI 14 13 12 13 14 14 0 0 1 0 0 0 NOT RE14ARKABLNEE:D: IS IIsS 1I5s ISs 1i5s 1I5s LUNG ILU) ............................................ NUMBE EXAMI:ED: 15 Is is Is is Is NOT !EMARKA LE: 14 Is 15 is Is Is MOTTLED LIVER ILIJ ...................................I.N.UM.B.ER...EXAMINED: NOT REMARKABLE: DIFFUSELY DARK 1 0 00 0 0 s is 15 Is 15 is Iis Is Is Is 14 Is LF. TESTIS 'SMALL (TEO) ..................................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 1 0 15 Is Is Is Is 15 Is Is 14 is 15 is PROSTATE (PR) .... ..........................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 0 0 10 0 0 Is Is is Is is Is 15 is is Is is 15 co HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INCIDENCE TABLE 23 OF 14ACROSCOPIC OBSERVATIONS I WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDESSEX-ALL;OROUP-ALL;WEEKS-ALL DEATH-2.SUBSEt-ALL N UM 6 ER 0 F A N IM A LS SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- -GORGAN AND KEYWORDISI OR PHRASE ------------------------------------------------------------NU-M-BE-R-: ----IS--- Is is Is Is Is SEMINAL VESICLE ISVI ............................N.UM.B.ER.n. EXAMINED: NOT E14ARKABLE: IS is Is I5s is is 15 Is Is 1 15 Is COAGULATING GLD (CGI ............................N.UM.B.ER.. EXAMINED: 15 15 IS 15 15 15 NOT REMARKABLE : IS is Is Is Is Is URETHRA JUR) ...................................NU.M.BE.R..E.XAMI:ED: IS Is 15 is Is Is NOT tEl4ARKA LE: 15 is Is is 15 15 SKIN (SKI .....................................NU.M.BE.R.RE.E.X.AMI:ED: 15 15 15 15 15 NOT MARKA LE: 15 Is Is Is 15 Is Is KIDNEYISI (KD) .................................N.L*.I.BE.R..EXAMINED: NOT REMARKABLE: LARGE PELVISIES) SPLEEN (SPI ... ................................N.UM.B.ER.I.tE.EXAMINED: NOT MARKABLE: 15 Is Is 15 is Is 15 is 14 15 Is Is 0 0 1 0 0 0 5 is Is is is is 1is Is is 15 Is is OD HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INCIDENCE TABLE 23 OF MACROSCOPIC OBSERVATIONS 8 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL NU H 8E R 0 F A N IMA LS SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -S- -6- OR-G-AN---A-ND--K-E-Y-WO-R-DI-S-)---O-N --PH-R-A-S-E-----------------------------------N-UM-B-ER-:-----I--S--- is 15 is is 15 I --- --- --- --- --- MESENTERIC LN (t4S) ..............................NU.M.BE.R..EXAMINED: NOT REMARKABLE: IS is is is is 15 15 Is Is 14 15 is MOTTLED 0 0 0 1 0 0 MARDIS/CERV LN (NNI .............................NU.M.BE.R..EXAMINED: NOT REMARKABLE: IS Is Is Is is 15 IS 13 IS 14 is 14 LARGE 0 2 0 1 0 1 LUMBAR/ILIAC LN ILM) ............................N.UM.B.EK.. EXA141PIED: 15 NOT REMARKABLE: 15 15 15 IS Is Is Is 15 IS Is Is END OF LIST HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. TABLE 24 INCIDENCE OF 14ACROSCOPIC OBSERVATIONS 14 WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE INCLUDESSEX-ALL GROUP-ALL;WEEKS-ALL DEATH-T;S'UBSET-ALL SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- -6- O-R-G-A-N--AN-D--K-E-YW-O-R-D-I-S)--O-R--P-H-R-A-SE--------------------------------N-U-M-B-ER-:----1--5------1-5--- is --- is --- -i-s- -I-s- 00 TOP OF LIST 66 GENERAL COMMENT IGCI ............................N.UM.B.ER.. EXAMINED: 15 Is Is 15 Is Is A LS ALL TISSUES/ORGiNS NORMAL 14 14 15 13 13 is BRAIN (OR) ........................................... NUMBER EXAMINED: 15 is Is is is Is NOT REMARKABLE: 15 Is Is Is is Is LUNG ILU) ......................................N.U.MB.ER EXAMINED: IS Is Is Is is Is NOT RE14ARKABLE: 15 is Is Is Is Is LIVER (Lt) ....................................N.U.M.B.E.R.E.XAMINED:15 Is is Is is Is NOT REMARKABLE: IS Is is Is is is LF. TESTIS (TEO) ...............................N.U.M.B.E.R.EXAMINED: IS is Is is Is is NOT REMARKABLE: IS 14 is Is is is SMALL 0 1 0 0 0 0 PROSTATE (PRI .... ..........................NU.0.4B.E.R EXAMINED: IS Is Is is is Is NOT REMARKABLE: IS Is Is 14 is is MASS(ES) 0 0 0 1 0 0 HAZLETON LABORATORIES . AMERICA, INC. MADISON. WISCONSIN U S.A. TABLE 24 INCIDENCE OF MACROSCOPIC OBSERVATIONS 14 WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;G*OUP-ALL;WEEKS-ALL DEAT"-T;SUBSET-ALL --- N U N 8 E R - 0 F - A N I M A L S SEX: -----------MA-LE ------------ GROUP: -I- -2- ORGAN AND KEYWORDIS) OR PHRASE --------------------------- NUMBER: 15 is 7 ------------------------------------------------a - -3- -4- -5- -615 Is 15 is --- --- --- --- SEMINAL VESICLE ISVI ...............................N.UMBEREXAMINED: 15 Is Is is 15 Is NOT REMARKABLE: 15 Is is is Is Is COAGULATING OLD (C-01 ................................. NUMBEREXAMINED: IS 15 15 NOT REMARKABLE: 15 Is 15 15 15 15 is Is Is URETHRA JUR) @ ........................................ NUNIIEElXtAMINED: 15 15 15 15 15 Is NOT ITEMARKABLE1:5 15 15 15 15 Is SKIN (SKI .....................................N.U.M.B.E.R.. EXAMINED: 15 Is Is Is Is Is NOT tEMARKABLE: IS Is Is is Is Is KIDNEY(S) (KD) .................................N.U.M.B.E.RE.XAMINED: 15 15 is is Is is NOT REMARKABLE: 15 Is Is 14 14 Is LARGE PELVIS(ES) 0 0 0 1 1 0 SPLEEN (SPI ........ I .......................N.U.M.B.EREXAMINED: 15 Is Is is Is Is NOT REMARKABLE: 15 Is Is Is Is Is MESENTERIC LN IMS) ......................N.U.M.B.EREXAMINED: 15 15 15 15 15 15 NOT REMARKABLE: is is is is Is Is HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. TABLE 24 INCIDENCE OF MACROSCOPIC OBSERVATIONS 14 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SIX-ALL GROUP-ALL;WEEKS-ALL DEAT"-T;S'UBSET-ALL U M BE 0 A N IM A L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- -6O-R-GA-N---AN-D--K-E-YW-O-RD-I-S-I ---OR--P-H-RA-S-E--------------------------------N-U-MB-E-R:----I-S----I-s- Is Is Is Is MANDIO/CERV LM LARGE LUM13AR/ILXAC LN END OF LIST IMM) .............................N.UM.B.ER..EXAMINED: NOT REMARKABLE: ILMI ............................N.UM.B.ER.. EXAMINED: NOT REMARKABLE: 15 is is Is 15 Is 14 Is Is is 14 Is 1 0 0 0 1 0 IS Is Is Is Is Is 15 15 IS IS Is is HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INCIDENCE TABLE 25 OF MACROSCOPIC OBSERVATIONS 22 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLT#DES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEAT".u; SUBSET-ALL SEX: GROUP: ORGAN AND KEYWORDIS) OR PHRASE -- --- -- ---- -- NUMBER: *6 TOP OF LIST *6 GENERAL COMMENT (OC) .............................NU.M.BE.R..EXAMINED: --- N U M 6 E N - 0 F - A N I M A L S -----------M-ALE -------- -I- -2- -3- -4- -5- 10 --- -1-0- -1-0- --to- -1-0- 10 10 10 to 10 ALL TISSUES/ORGANS ANIMAL THIN AN104AL OBESE NORMAL BRAIN JOR) ........................................... 1 10 0 9 1 0 0 0 0 1 0 0 1 0 0 NtR48ER EXAMINED: 10 10 10 to to NOT REMARKABLE: 10 to 10 10 10 LUNG ILU) ...............I...........................NU.MBER EXAMINED: 10 10 to 10 10 NOT RE14ARKABLE: 10 10 10 10 10 LIVER ILI) ....................................N.U.M.B.E.R.E.XAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 to to LF. TESTIS (TEO) .........................N.U.M.B.EREXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 to 10 PROSTATE (PR) ..... HASSIES) .......................N.U.MB.E.R EXAMINED: 10 10 10 10 to NOT REMARKABLE: 10 10 10 to 9 0 0 0 to "AZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. TABLE 25 INCIDENCE OF MACROSCOPIC OBSERVATIONS 22 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX&ALL;GROUP-ALL;WEEKS-ALL DEAT"-U;SU13SET-ALL SEX: GROUP: NU M 8E R 0 F -A N IMA LS -----------M-ALE ------------I- -2- -3- -4- -5- -6- O-R-G-AN---AN-D--K-E-YW-O-R-DI-S-)---OR--P-"-R-AS-E--------------------------------N-U-MB-E-R:-----1--0------1.0--- 10 -.- 10 -a- 10 -*- -.0- SEMINAL VESICLE (SV) ............................N.UM.B.ER.. EXAMINED: 10 10 to 10 10 0 NOT REMARKABLE: 10 10 to 10 10 0 COAGULATING GLD ICOI ........... @.. NUMBER EXAMINED: 10 10 10 10 10 0 NOT REMARKABLE: 10 10 10 10 10 0 UNET"RA JUR) ........................ ...........N.UMBER EXAMINED: 10 10 10 10 to 0 NOT REMARKABLE: 10 10 to 10 10 0 SKIN (SKI .....................................N.UM.13.E.R..EX.AMINED: 10 10 10 to 10 0 NOT REMARKABLE: 9 10 10 10 10 0 ABRASION(S)/ULCERATIONISI 1 0 0 0 0 0 KIDNEYIS) IKDI .................................N.UM.1.3E.M..EXAMINED: 10 to 10 10 10 0 NOT REMARKABLE: 10 10 10 9 to 0 LARGE PELVIS(ESI SEMIFLUID MATERIAL-PELVIS 0 0 0 1 0 0 0 0 0 1 0 0 SP@EEN ISPI ................ .................N.UM.BER EXAMINED: 10 10 10 10 10 0 NOT REMARKABLE: 9 10 10 10 10 0 CYSTIS) 1 0 0 0 0 0 00 HAZLETON LABORATORIES AMERICA, INC. t4ADISON. WISCONSIN U.S.A. TABLE 2S INCIDENCE OF MACROSCOPIC OBSERVATIONS 22 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-U;SUBSET-ALL N U M 8 E R - 0 F - A N IM A L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- -6- ORG-A-N--A-ND--K-E-Y-WO-R-D-(S-)---O-R--PH-R-A-SE---------------------------------NU-M-BE-R-: ----10----1-0- 10 10 10 0 HESENTERIC LN (MS) ..............................NU.M.BE.R..EXAMINED: 10 10 10 10 10 0 NOT REMARKABLE: 10 10 10 10 10 0 MANDIS/CERV LN INN) .............................N.UM.B.ER.. EXAMINED: 10 10 to 10 to 0 NOT REMARKABLE: 10 10 to 10 9 0 LARGE 00 0 0 1 0 LUMBAR/ILIAC LN ILM) ..............................NU.M.BER EXAMINED: 10 10 10 10 10 0 NOT REMARKABLE: 10 10 10 10 9 0 LARGE 1 END OF LIST 0 0 0 0 1 0 o-n HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. INCIDENCE TABLE 26 OF MICROSCOPIC OBSERVATIONS 5 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;OROUP-ALL-SCREEN-ALL;WEEKS-ALL DEAT"-I;FIND-ALL.tUBSIET-ALL --- " U M 6 E R - 0 F - A N I H A L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- O-R-GA-N --AN-D- F-I-N-DI-N-G - DESCRIPTION ** TOP OF LIST *6 NUMBER: -1-5- 15 -a- -1.5- 15 -.- 14 -.- Is -.- BRAIN IOR) ....................................N.U.M.B.E.R.E.XAMINED: 15 Is is 15 14 15 NOT ItEt4ARKABLE: IS Is is Is 14 15 LUNG (LU) .....................................N.U.M.B.E.R.E.XAMINED: 15 15 IS 14 14 Is NOT REMARKABLE: 15 is Is 14 14 15 LIVER ILI) ......... .. ...... .......NUMBER EXA14INED: IS 15 IS 15 14 15 NOT REMARKABLE: 9 7 2 0 a 10 --INFILTRATES.MONONUCLEAR CELL INFLAMMATION GRANULONATOUS --NECROSIS COADULATIVE --FATTY C"ANGE "YPERTROP"Y NEPATOCELLULAR: CHARACTERIZED BY CENTRILOBULATED PATTERN IN WHICH THE "EPATOCYTES AN ACCENTUATED APPEAR TO HAVE 5 7 4 s 5 4 III1 0 0 0 0 0 1 2 1 0 0 0 0 1 00 0 12 15 14 0 A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANCE. EXCEPT FOR THIS *ENLARGED" APPEARANCE, THE CELLS ARE OTHERWISE *NORMAL". --"YPERPLASIA,BILE DUCT 0 0 0 0 0 0 LF'.TESTIS (TEO) ............. . . .....NUMBER EXAMINED: 15 Is 15 Is 14 Is NOT REMARKABLE: IS is 15 15 13 is --ATROPHY/DEGENERATION --INFLAMMATION. GRANULOMATOUS 0 0 0 0 1 a 0 0 0 0 0 0 INCIDENCE TABLE 26 OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMIRICA, INC. MADISON. WISCONSIN U S.A. 5 WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;OROUPeALL;SCREEN-ALL;WEEKS-ALL DEATH-I;FIND-ALL;SUBSET-ALL IN L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- -G- OR(IAN AND FINDING DESCRIPTION NUMBER: Is IS IS ---------------------------------------------------------------------- IS 14 Is -0- --- --- -*- -.- PROSTATE IPRI ..................................N.U.M.B.E.R.E.XAMINED: 15 IS 14 15 14 15 NOT REMARKABLE: IS 15 14 IS 14 13 --INFLAMMATION. CHRONIC ACTIVE 0 0 0 0 0 2 SEMINAL VESICLE ISVI ............................N.U.M.B.E.REXAMINED: 15 15 14 IS 14 15 MOT R114ARKABLEI:S Is 14 IS 14 Is COAGULATINO OLD ICO) ............................N.U.M.B.ER.R,EXAMINED1:5 IS 14 15 14 15 NOT t4ARKABLE : 15 Is 14 15 14 Is URETHRA (URI ..................................N.U.M.B.E.R.E.XAMINED: 15 15 14 15 14 14 NOT REMARKABLE: 15 Is 14 IS 14 14 KIDNEY(S) IKD) .................................N.U.M.B.E.R.EXAMINED: 1 2 0 2 3 1 NOT RE14ARKABLE: 0 0 0 0 0 0 --DILATATION.PELVIC 1 2 0 2 3 1 SKIN ISK) .................. .................N.U.MBEREXAMINED: 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 --DERNATITIS --ULCERATION 0 0 1 0 0 0 0 0 0 0 0 0 INCIDENCE TABLE 26 OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. 5 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL@GROUP*ALL;SCR [IN-ALL;WEEKS-ALL DEATH-I;FIND-ALL.SUBSET-ALL --- N U N 8 E R - 0 F - A N I M A L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- -s- O-R-G-AN---AN-D--F-I-ND-I-N-G --D-E-SC-R-I-PT-I-O-N----------------------------------NU-M-BE-R-: ----15----1-5 - Is Is 14 Is --- --- --- --- -a- SPLEEN ISPI ........ :............................N.U.MB.E.R . EXAMINED: 0 00 1 0 0 NOT REMARKABLE: 0 00 1 0 0 --CYST. SEROSAL 0 0 0 0 0 0 MANO'BICERV LM ("Ni ..... .. .......................NUMBER'IEXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --HYPERPLASIA. LYMP"ORETICULAN CELL MESENTERIC LN IMS) .............................N.UM.B.ER..EXAMINED: NOT RE04ARKABLE: 0 00 00 0 0 0 0 0 0 0 0 0 0 0 0 0 LUMBAR/ILIAC LN (LMI ............................N.UM.B.ER.. EXAMINED: 0 00 0 0 0 NOT REMARKABLE: 0 00 0 0 0 --"YPE PLASIA, LYMPHORETICULAR END OF 0LIST CELL 0 0 a 0 0 0 HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. INCIDENCE TABLE 27 OF MICROSCOPIC OBSERVATIONS I WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEXOALL GIOUP-ALL-SCREEN-ALL;WEEKS-ALL I)EATH-27F MD-ALL;@UBSET-ALL --- N U N B E R - 0 F - A N I M A L S SEX: -----------MA-LE ------------ GROUP: -I- -2- OftOAN AND FINDING DESCRIPTION - ---------------------------------------------------N-U-MB-E-R-:----1--5-----Is- 04 TOP OF LIST 04 --- BRAIN JOR) ........:.............................N.U.M.B.E.REXAMINED:15 IS NOT REMARKABLE : IS IS -3is --- iIsS -4is --15 Is -5-i-sis Is -a- -I-s- Is Is LUNG (LUI ............................................ NUMBEREXAMINED: 14 NOT REMARKABLE IS 3 14 Is Is : 14 IS 113 14 IS 15 LIVER (LI) ....................................N.U.M.B.E.R.E.XAMINED: 15 IS NOT RE14ARKABLE: It 12 IS 3 15 0 Is 0 Is 10 --INFILTRATES,MONONUCLEAR CELL INFLAMMATION GRANULONATOUS 4 3 1 45 5 --NECROSIS COADULATIVE 0 0 0 1 0 0 -FATTY C"AN(IE 0 0 0 0 1 0 --"YPENTROPHY, "EPATOCELLULAR: CHARACTERIZED BY AN ACCENTUATED 0 0 0 0 0 0 CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTES APPEAR TO HAVE 0 0 12 Is is 0 A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANCE. EXCEPT FOR THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTHERWISE -NORMAL-. --"YPERPLASIA.BILE DUCT LF. TESTIS (TEO) ................ --ATROPHY/DEGENERATION 0 0 0 0 0 0 .........I.... NUMBER EXAMINED: 5 Is 15 Is Is is NOT REMARKABLE: 11 5 15 14 is Is Is -INFLAMMATION, GRANULONATOUS 0 1 0 0 0 00 0 0 0 0 0 to to INCIDENCE TABLE 27 OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMEMICA, INC. MADISON. WISCONSIN U.S.A. I WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GAOUP-ALL-SCREEN-ALL;WEEKS-ALL DEATH-2;FIND-ALL;JUBSET-ALL NUM 8 E R 0 F AN IN A LS SEX: -----------MA-LE ------------ GROUP: -I- -2- -3- -4- -5- -GO-R-G-A-N --AN-D--FI--I-N-DI-N-G--D-E-S-CR-I-P-T-IO-N----------------------------------NU-M-B-E-R:-----I-s-----i-s Is Is is Is -.- -.- -.- -.- -*- PROSTATE IPRI ..................................N.U.M.B.E.RE.XAMINED: 15 is is Is Is is NOT REMARKABLE: 14 15 IS 15 14 15 --INFLAMMATION. CHRONIC ACTIVE 1 00 0 1 0 SEMINAL VESICLE ISVI ............................N.U.M.B.E.REXAMINED: 15 Is Is is 15 is NOT REMARKABLE: IS Is Is Is is Is COAGULATING GLD ICG) ............................N.U.M.B.EREXAMINED: 15 Is 15 Is is is NOT REMARKABLE: 15 Is Is Is Is is URETHRA (URI ..................................N.U.MB.E.R..E.XAMINED: 15 Is is 14 14 14 NOT REMARKABLE: is Is Is 14 14 14 KIDNEVIS) (KDI .................................NU.M.BE.R..E.XAMINED: 0 0 1 0 0 0 140T RE14ARKABLE: 0 0 0 0 0 0 --DILATATION.PELVIC skin (SKI ................ ..................N.U.MB.ER EXAMINED: NOT REMARKABLE: a 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 --DERMATITIS ULCERATION 0 0 0 0 0 0 0 0 0 0 0 0 C3 INCIDENCE TABLE 27 OF MICROSCOPIC OBSERVATIONS "AZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. I WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROIUP-ALL-SCREEN-ALL;WEEKS-ALL DEATH-2;F NO-ALL:iUBSET-ALL N U N 13E R 0 F A N I H A L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- -GO-R-G-AN---AN-D--F-I-ND-I-N-G --D-E-SC-R-IP-T-I-ON----------------------------------N-U-MB-E-R:----1-5----I-s- is is Is Is --- --- --- --- --- SPLEEN (SPI .......:.............................N.U.MB.E.R . EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 00 0 0 --CYST. SEROSAL 0 0 0 0 0 0 MANDIO/CERV LN INNI .............................N.UM.BE.R.'. EXA14INED: 0 2 0 1 0 1 NOT REMARKABLE: 0 0 0 0 0 0 --NYPERPLASIA, LYMPHORETICULAR CELL MESENTERIC LN (MS) ..............................NU.M.BE.R..EXAMINED: NOT REMARKABLE: 0 2 0 1 0 1 0 0 0 1 0 0 0 0 0 1 0 0 LUMBAR/ILIAC LM --HYPERPLASIA. END Of LIST (LM) ............................N.UM.B.ER..EXAMINED: NOT REMARKABLE: LYMPHORETICULAR CELL 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 INCIDENCE TABLE 21 OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. 14 WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;OROUP-ALL.SCREEN-ALL;WEEKS-ALL DEATH-T;FIND-ALL;SUBSET-ALL N U M 8 E R 0 F - A N IM A L S SEX: -----------K-ALE ------------ OROUP: -I- -2- -3- -4- -5- -G- ORGAN AND FINDING DESCRIPTION NUMBER: 15 15 Is -sm--T-O-P -O-F--L-I-ST---am---------------------------------------------------------------- --- BRAIN ISK) ............................................ NUMBER EXAMINED: IS Is is NOT REMARKABLE: IS IS 15 Is --- is IS is --- Is IS Is --- Is Is LUNG (LU) .....................................N.U.M.B.E.R.E.XAMINED: 15 IS 15 15 IS 15 NOT REMARKABLE: 15 is Is Is is is LIVER ILI) ....................................N.U.MB.E.R ..E.XA14INED: Is Is Is Is is Is NOT RE14ARKABLE: 12 11 2 1 0 7 --INFILYRATES.MONGNUCLEAR CELL --INFLAMt4ATION GRANULOt4ATOUS --NECROSIS COADULATIVE --FATTY C"ANCIE --HYPERTROPHY "EPATOCELLULAR: CHARACTERIZED BY AN ACCENTUATED CENTRILOSULATED PATTERN IN WHICH THE HEPATOCYTES APPEAR TO HAVE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE ROUNDED GIVING THE CELLS A MORE "PLUMP" APPEARANCE. EXCEPT FOR THIS "ENLARGED' APPEARANCE. THE RFILS ARE OTHERWISE "NORMAL". --HYPERPLASIA.BILE DUCT 3 3 0 5 7 0 0 0 1 30 0 1 0 1 0 0 00 0 0 0 0 0 0 0 13 14 15 0 0 0 0 0 0 0 Le. TESTIS (TEO) ................... ...........N.UMBER EXA14INED: 15 Is is Is is Is NOT REMARKABLE: 15 14 15 15 15 is --ATROPHY/DEGINERATION --INFLAMMATION. GRAMULOMATOUS 0 1 0 0 a 0 0 1 0 0 0 0 INCIDENCE TABLE 20 OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. 14 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: --- N U M 8 E R - 0 F - A N I H A L S SEXoALL;GROUP;ALL-SCREEN-ALL;WEEKS-ALL DEATH-T;FIND- LL;TUBSET-ALL SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -S- -6- ORGAN--A-N-D --FI-N-D-IN-G--D-E-S-CR-I-P-TI-O-N---------------------------------N-U-M-BE-R-:----I--S-----I-s- is is Is Is --- --- --- --- -*- PROSTATE (PRI ......: ............................N.U.M.B.E.REXAMINED: IS IS IS 15 15 15 NOT REMARKABLE: IS 15 13 14 14 14 --INFLAMMATION. CHRONIC ACTIVE 0 0 2 1 1 1 SEMINAL VESICLE ISVI ............................N.U.M.B.E.RE.XAMINED: IS Is is is Is Is NOT RE14ARKABLE: 15 Is Is Is Is Is COAGULATING GLD ICO) ............................N.U.M.B.E.REXAMINED: 15 is is Is is is NOT REMARKABLE: 15 Is 15 Is Is Is URETHRA (URI ..................................N.U.M.B.E.R.E.XAMINED: 15 Is is Is Is Is NOT REMARKABLE: IS Is Is Is Is Is KIDNEY(S) IKDI .................................NU.M.BE.R..E.XAMINED: NOT REMARKABLE: --DILATATION,PELVIC SKIN fSK) ................... --DERNATITIS --ULCERATION ................N.U1.4BER EXAMINED: NOT REMARKABLE: 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 0 0 0 C3 w INCIDENCE TABLE 20 OF MICROSCOPIC OBSERVATIONS HAZLETO" LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. 14 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES. SEX-ALL;GROUP-ALL-SCREEN-ALL;WEEKS-ALL DEATH.T;FIND-ALL;tUBSET-ALL SEX: GROUP: N U M 6 E N -0 F -A N IM A L S -----------M-ALE ------------I- -2- -3- -4- -5- -G- ORGAN AND FINDING DESCRIPTION NUMBER: IS --------------------------------------------------------------------- -I-s- -I-s- -i-s- Is -*- Is -.. SPLEEN ISP) ....................................NU.M.BE.R..E.X.AMINED. 1#01 RE14ARKABLE: 0 0 0 0 0 0 0 0 0 0 a 0 --CYST. SEROSAL 0 0 0 0 0 0 MANDIS/CERV LM (14N) .............................H.UM.B.lE.i. EXA14INED: 1 0 0 0 1 0 NOT RE14ARKABLE: 0 0 0 a 0 0 --HYPERPLASIA, LYMPHOITETICULAR CELL 1 0 0 0 1 0 MESENTERIC LN IMS) ..............................NU.M.BE.R..EXAMINED: NOT RE04ARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 LUMBAR/ILIAC LN (LMI ... ....................NU.M.BE.R EXAMINED: NOT REMARKABLE: --HYPERPLASIA. LYMPHORITICULAN CELL END OF LIST 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 INCIDENCE TABLE 29 Of MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. 22 WEEK SACRIFICE ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL GIOUP-ALL-SCREENnALL;WEEKS-ALL DEATH-U;F' ND-ALL;tUBSET-ALL SEX: GROUP: N U M 8 t R -0 f - A N I M A L S -----------1-4ALE ------------I- -2- -3- -4- -S- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 to to 10 to 0 ------------------------------------------------------------------------ 00 TOP OF LIST &A BRAIN (ON) .........:...........................N.t.*.*E.f.t EXAMINED: 10 1t0o 9 10 10 0 NOT REMARKABLE: 10 9 to In 0 LUNG ILU) .....................................N.U.M.B.E.R.E.XAMINED: 1to0 10 10 10 10 0 1401 REMARKABLE: to 10 to 10 0 LIVER ILI) ....................................r.iu.m.el.E.O'.E.XAMINED: 10 to 10 10 10 0 NOT RE04ARKABLE: s s 6 17 0 --INFILTRATES,MONONUCLEAR CELL --INFLAMMATION GRANUL004ATOUS --NECRCISIS COADULATIVE --FATTY CHANGE HYPE:TROP"Y HEPATOCELLULAR: CHARACTERIZED BY AN ACCENTUATED CENT I OBULATED PATTERN IN WHICH THE NEPATOCYTES APPEAR TO HAVE A MORE HOMOGENEOUS CYTOPLAS14 AND THE CELL BORDERS ARE MORE ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANCE. EXCEPT FOR THIS 'ENLARGED" APPEA*ANCE, THE CELLS AN[ OTHERWISE -NORMAL-. --NYPERPLASIA.BILE DUCT 2 4 2 1 3 0 2 0 0 0 0 0 I I I 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 LF. TESTIS (TEO) .................. ...........N.UMBER EXAMINED: 10 10 10 10 10 0 NOT RE04ARKABLE: 10 9 9 9 to 0 --A7ROPHV/DEGENERAYTON --INFLAMMATION. GRANULONATOUS 0 1 1 1 0 0 0 0 0 0 0 0 INCIDENCE TABLE 29 OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. 22 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES, SEX-ALL-GROUP*ALL SCREEN-ALL.WEEKS-ALL PEAT"-U;FIND-ALL;tUBSET-ALL I LS SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -S- -$- to to ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 10 0 ------------------------------------------------------------------------ PROSTATE IPO) ......: ..............................N.UNMO.BTERRE1E4XAARMKIANBELDE:: 10I @, 1100 ttoo 1t0o 101 00 --INFLAMMATION, CHRONIC ACTIVE 2 0 0 0 2 0 SEMINAL VESICLE (SVI ...................... ....NUMBER EXAMINED: 10 10 to to 10 0 NOT REMARKABLE: 10 10 10 10 10 0 COAGULATING OLD (CO) .@...... ..................NUMBER EXAMINED: 10 10 to 10 10 0 NOT REMARKABLE: 10 10 10 10 10 0 URETHRA (URI ...... to .................NUMBER EXAMINED: 10 10 10 10 0 NOT REMARKABLE: 10 10 10 10 10 0 KIDNEY(SI (KDI .................................O.TM.E.N ..E.XAMINED: 0 0 0 10 0 NOT RE14ARKABLE: 0 0 0 00 0 --DILATATION.PELVIC 0 0 0 10 0 SKIN lsxl ............... ....................... NUMBER EXAMINED: NOT REMARKABLE: 1 0 0 00 0 0 00 0 0 0 --DERHATITIS --ULCERATION t0 0 0 0 0 1 00 00 0 INCIDENCE TABLE 29 OF MICROSCOPIC OBSERVATIONS HAZLETON LA130RATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. 22 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------- TABLE 114CLUDES: SEX-ALL;GROUP-ALL-SCREEN-ALL;WEEKS-ALL DEATH-U;FlffD-ALL;UBSET-ALL SEX: GROUP: NUN 8E R -0F -A N IMA L S -----------M-ALE ------------I- -2- -3- -A- -S- -a- ORGAN AND FINDING DESCRIPTION HUMOER: 10 10 10 10 10 0 ---------------------------------------------------------------------------- --- --- --- --- SPLEEN ISP) ... ................................N.U.M.B.E.R.E.XAMINED: 1 0 0 0 0 0 NOT RE14ARKAIILE: 0 0 0 0 0 0 --CYST. SEROSAL 1 0 0 0 0 0 MANDIO/CERV LN (NMI .................................NUMBER EXAMINED: NOT REMARKABLE: 0 00 0 1 0 0 0 0 0 0 0 --HYPERPLASIA, LYMPHOPETICULAR CELL 0 00 0 1 0 MESENTERIC LM (HSI ..............................NU.M.BE.R..EXAMINED: NOT REMARKABLE: 0 0 0 0 00 0 0 0 00 0 LUMBAR/ILIAC LN (LNI ............................N.U.MB.E.R . EXAMINED: 0 00 0 1 0 NOT REMARKABLE: 0 0 0 0 a 0 --HYPERPLASIA, END OF LIST LYMPHORETICULAR CELL 0 0 0 a 1 0 INCIDENCE TABLE 30 OF SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. S' WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP*ALL;SCREEN-ALL;WEEKS-ALL DEATH-I;FIND*ALL;SUBSET-ALL NU M 6E R -0 F -A N IMA L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -S- -6- O-R-(-IA-N-/-TI-S-S-UE---EX-A-M-IN-E-D----------------------------------------N-U-MB-E-R:------1-5-----Is-- 15 Is 14 Is 0* TOP OF LIST 4* BRAIN (BRI ....................................N.U.M.BE.R..E.X.AMINED: is Is Is Is 14 15 LUNG ILU) .....................................NU.M.BE.R...EX.AMINED: 15 IS IS 14 14 IS LIVER (LIJ ....................................N.U.MS.E.R..E.XA.MINED: IS 15 15 15 14 15 --INFILTRATES.MONONUCLEAR CELL 10 1 11 10 9 It 1) 3 s 4 5 4 3 2) 2 2 00 1 1 TL@ 15 Is Is is 14 Is --INFLAMMATION.GRANULONATOUS -) 14 14 14 14 14 is 1) 0 1 1 00 0 2) 0 0 0 1 0 0 3) 1 0 0 0 0 0 TL@ IS Is IS IS 14 Is --NECROSIS.COAGULATIVE --FATTY CHANGE CONTINUED ON NEXT PAGE -) 15 IS 15 14 12 14 2) 0 0 0 0 1 1 3) 0 0 0 1 1 0 TL) IS IS IS IS 14 is -) is Is Is is 14 14 2) 0 0 0 0 0 1 TL) IS Is Is Is 14 Is co INCIDENCE TABLE 30 OF SEVERITY OF MICROSCOPIC OBSERVATIONS 14AZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. 5 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP;ALLiSC:EEN-ALL;WEEKS-ALL DEATH-I;FIND- LL; UB ET-ALL --- N U M 8 E R - 0 F - A N I M A L S SEX: -----------MA-LE ------------ GROUP: -I- -2- -3- -4- -5- -a- O-R-G-AN-/-T-IS-S-U-E ---EX-A-M-IN-E-D----------------------------------------N-L-*4-8-E-R:---1-5----I-s- is Is 14 Is 60 FROM PREVIOUS PA()E,ma LIVER ILI@ ........i.Ai&iii6iAi:..................N.U.M.BEREXA14INED:15 IS --HYPE TROPHY HE 15 Is 14 is CHARACTERIZED BY AN ACCENTUATED -) 15 15 CEt4TRILODULATED PATTERN IN WHICH THE #I[PATOCYTES APPEAR TO "AVE l@ 30 0 Is A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE 0 0 12 7 s 0 2), 0 0 0 a 9 0 ROUNDED GIVING THE CELLS A MORE "PLUMP* APPEARANCE. EXCEPT FO TL) IS THIS 'ENLARGED" APPEARANCE, THE CELLS ARE OTHERWISE -NORMAL-. 15 Is Is 14 Is --HYPERPLASIA,BILE DUCT -) 15 15 15 IS 14 IS TL) 15 Is Is Is 14 Is LF. TESTIS 17EO) ...............................N.UM.BE.R...EXAMINED: --AIROP"Y/DEGENERATION 15 is 15 IS 14 15 -) is is Is is 13 is 3) 0 0 0 0 1 0 TL.I- 15 is 15 15 14 Is --INFLAMMATION, GRAMULONATOUS -) Is Is is Is 14 is IL) IS is Is Is 14 is PROSTATE IPR --INFL NUMBER EXAMINED: 15 15 14 is 14 is 15 15 14 Is 14 13 2) 0 0 00 0 1 3@ 0 0 00 0 1 TL) 15 Is 14 is 14 is SEMINAL VESICLE (SVI ...... ...................NUMBER EXAMINED: 15 Is 14 Is 14 Is to INCIDENCE TABLE 30 OF SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. S WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;G*OUP;ALLiSCREEN-ALL;WEEKS-ALL DEAT"-I;FIND- LL; UBSET-ALL SEX: GROUP: u M 13E R - 0 f - A N I M A L S -----------MA-LE ------------I- -2- -3- -4- -S- -6- O-R-G-AN-/-TI-S-S-UE----EX-A-M-IN-E-D----------------------------------------N-U-MB-E-R:----I-S-----t--s-- is --- is --- 14 --- Is --- COAGULATING GLD IPGI ............................N.U.M.B.E.REXAMINED: 15 Is 14 Is 14 is URETHRA JUR) ..................................N.U.M.B.E.R.E.XAMINED: IS 15 14 IS 14 14 KIDNEYIS) NUMBEREXAMINED: 1 2 0 2 3 1 --DILATilDllt4:Oii@i.6 ............................... P). 1 2 0 2 3 1 TL) 1 2 0 2 3 1 SKIN ISK) .....................................".U.M.B.E.RE.X.AMINED: 0 0 1 0 0 0 --DERMATITIS 3), 0 0 1 0 0 0 TL) 0 0 1 0 0 0 --ULCERATION 0 0 1 0 0 0 TL) 0 0 1 0 0 0 SPLEE!CYLIP,S,,b,A@ ...............................NU.M.BE.R..EXAMINED: 0 0 0 1 0 0 T. 0 0 0 1 0 0 TL@ 0 0 0 1 0 0 t4AND184CEENVPLANSIIMN I ..................... .......NUMBER EXAMINED: 0 0 0 0 0 0 YP A L LYMPHORETICULAR CELL TL), 0 0 0 0 0 0 MESENTERIC LN (MSI ... .......NUMBER EXAMINED: 0 0 0 0 0 0 LUMBAR/ILIAC LM (LMI ............ --HYPERPLASIA, LYMP"ORETICULAR END Of LIST .....NUMBER EXAMINED: 0 0 0 0 0 0 TL) 0 0 0 0 0 0 TABLE 31 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLE70M LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. I WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE INCLUDES@ SEX-ALL;GROUP@ALL@SCREEN*ALL;WEEKS-ALL DEATH-2:FIND-ALL: USSET-ALL SEX: GROUP: N U M 0 E R -0 F - A N I M A L S -----------MA-LE ------------1- -2- -3- -4- -5- -6- OROAN/TXSSUE EXAMINED NUMBER: 15 15 IS IS 15 Is ------------------------------------------------------------------------------ --- --- - 6* FROM PREVIOUS PAGE me LIVER ILI NUMBER EXAMINED: 15 Is is Is Is is -HYPEAP-L-A-S.I.A...B.I.LE.-D.U...C..-T......................... ) 15 Is Is Is Is Is TL-II is is is is Is Is LF. TESTIS ITEO) ...............................N.U.MB.ER...EXA14INED-. IS is is is is Is ATROPHY/DEGENERATION -) Is 15 14 IS is Is 4) 0 0 1 00 0 TL@ IS Is Is Is Is is --INFLAMMATION. GRANULOMATOUS Is Is is 15 15 Is TL), Is is is Is Is Is PROSTATE IPA) ....................................".".ER. EXAMINED: IS is is is is Is --INFLAMMATION. CHRONIC ACTIVE -) 14 Is Is Is 14 Is 2) 1 0 0 0 0 0 3) 0 0 0 0 1 0 TLI, IS is is Is Is is SEMINAL VESICLE ISV) .... COAGULATING OLD ICO) .... UKETHRA JUR) ............ ...................N.UM.B.ER ...................N.UM.B.ER .......I............N.UMBER EXAMINED: EXAMINED: EXAMINED: 15 Is is Is is is IS is Is Is is is 15 15 Is 14 14 14 INCIDENCE TABLE 31 Of SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. I WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE 114CLUDES: SIX-ALL@GNOUP;ALL-SCREEN-ALL;WEEKS-ALL DEATH-2;FIND- LL;TUBSET-ALL SEX: -----------M-ALE -----------GROUP: -I- -2- -3- -4- -5- -fi- ORGAN/TISSUE EXAMINED NUMBER: 15 IS IS Is ts is --------------------------------------------------------------------------- --- --- --- --- && TOP OF LIST *0 BRAIN (13RI ....................................N.U.M.B.E.R.E.XAMINED: IS 15 Is Is Is 15 LUNG (LUI . ....................................N.U.M.BEREXAMINED: 14 Is 13 14 Is Is LIVER ILI) ....................................N.U.M.B.E.R.E.XAMINED: IS Is 15 Is 1to5 is --INFILTRATES.MONO"UCLEAR CELL -) It 12 14 11 10 l@ 4 3 0I 4 3 3 2) 0 a 0 2 2 TL@ 15 15 Is 15 Is is --INFLAMMATION,GRAMULONATOUS -) 15 15 15 14 15 is 1) 0 0 0 1 0 0 TL) IS is Is Is Is Is --NECROSIS.COAGULATIVE -k Is Is 15 15 14 Is 2) 0 0 0 0 1 0 TL@ IS Is 15 is is is --FATTY CHANGE -) 15 TL) 15 is Is Is Is Is Is Is Is is Is --HYPERIRDPHY NEPATOCELLULAR: CHARACTERIZED BY AN ACCENTUATED -k Is Is 3 0 0 Is CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES APPEAR TO HAVE 1) 0 0 12 14 0 0 A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE "ONE 2) 0 0 0 1 9 0 ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARANCE. EXCEPT FO TL) 15 Is is Is Is Is THIS 'ENLARGED' APPEARANCE. THE CELLS ARE DIHERWISE 'NORMAL'. CONTINUED ON NEXT PAGE INCIDENCE TABLE 31 OF SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. I WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL-OROUP-ALL-SCREENeALL;WEEKSuALL DEATH-27FINO-ALL;tUBSET-ALL SEX: GROUP: N UH 0E R -0 F -A N IH A LS -----------M-ALE ------------I- -2- -3- -4- -5- -6- ORGAN/TISSUE EXAMINED NUMBER: IS is Is 15 Is is --------------------------------------------------------------------------- --- -a - --- --- KIDNEY TIKOI .....: ............................N.UM.B.ER.. EXAMINED-. 0 0 1 0 0 0 --@SIILA TION.PELVIC P) 0 0 1I 0 0 0 TL), 0 0 0 0 a SKIN__ISKI .....................................N.UM.B.ER...EX.AMINED: 0 0 0 0 0 0 ERNATITIS IL)- 0 0 0 0 0 0 --ULCERATION TL), 0 0 0 D 0 0 SPLEEN YLSPI ...................................N.UM.BE.R...EX.AMINED: 0 0 0 0 0 0 --C T. SEROSAL TL@ 0 0 0 0 0 0 14AND164CERV LM INN) ..............................NU.MB.E.R EXAMINED: 0 2 0 1 0 1 -- YPERPLASI , LYMP"ORETICULAR CELL 3@ 0 2 0 1 0 1 TL@ 0 2 0 1 0 1 HESENTERIC LM INS) ..............................NU.M.BE.R..EXAMINED: 0 0 0 1 0 0 LUMBAR/ILIAC LN ILM) ............................N.UM.BE.R.. EXAMINED: 0 0 0 0 0 0 --HYPIERPLASTA. LYMPHORETICULAR CELL END OF LIST TL) 0 0 0 0 0 0 INCIDENCE TABLE 32 OF SEVERITY OF MICROSCOPIC OBSERVATIONS "AZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. 14 WEEK SACRIFICE -------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL SCREEN-ALL;WEEKS-ALL DEAT"-T;FIND-ALL@iUBSET-ALL SEX: GROUP: N U M 8 E R -0 F -A N IM A L S -----------M-ALE ------------1- -2- -3- -4- -5- -$- ORGAN/TISSUE EXAMINED NUMBER: 15 15 Is 15 is is --------------------------------------------------------------------------- 00 TOP OF LIST 04 BRAIN (BRI ........................................... NUMBER EXAMINED: 15 Is is Is Is is LUNG (LU) .....................................N.U.M.B.E.R.E.XAMINED: 15 Is Is is Is Is LIVER ILI) ..................... --IMFILTRATES.MONONUCLEAR CELi'* ... HUMBEiEtXAMINED: 15 15 15 IS 15 15 -) 12 12 9 10 1 7 1) 2 3 5 4 7 6 2) 1 0 1 1 0 2 TLk IS Is Is Is Is is --INFLAMMATION.GRANULONATOUS -) IS IS 14 12 15 15 11 0 0 1 2 0 0 2@ 0 0 0 1 00 TL@ IS Is Is Is Is is --NECROSIS.COAGULATIVE --FATTY CHANGE CONTINUED ON NEXT PAGE -) Is 14 Is 14 is Is TL2P) 150 151 I0S IS1 150 I0S -) Is Is Is Is Is Is TL) 15 Is Is 15 Is Is 4bb INCIDENCE TABLE 32 OF SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. 14 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX*ALL; OMOUP;ALLiSCREEN-ALL;WEEKS-ALL DEATH-T:FIND- LL; UBSET-ALL SEX: GROUP: N U M 8 E R - 0 F - A N IM A L S -----------M-ALE ------------I- -2- -3- -4- -5- -6- ORGAN/TISSUE EXAMINED NUMBER: IS is is is Is Is -------------------------------------------------------------------0-------- --- --- - 06 FROM PREVIOUS PAGE 00 LIVER (LI ........NUMBEREXAMINED: 15 Is is Is Is is -HYIPEITR..O.P.H.Y..H-E.-PAI-T-OC.E..LL.-UL.A.R..:-.C.-HA.R.AB.YC.-ATlEl.RA.C1C-EZN.TEUVATED -) Is Is 2 1 0 is CENTRILOSULATED PATTERN IN WHICH THE NEPATOCYTES APPEAR TO HAVE 1), 0 0 13 12 3 0 A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE 2) 0 00 2 12 0 ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANCE. EXCEPT FO TL@ IS is Is is is is THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHERWISE 'NOR14AL". --HYPERPLASIA.BILE DUCT ) 15 Is Is Is 15 is TL-) 15 Is is is Is is LF. TESTIS ITEO) ...............................N.U.MB.ER...EXAMINED: 15 is 15 is is is --ATROPHY/DEGENERATION -11 Is 14 Is is Is 15 3) 0 1 0 0 0 0 TL) IS Is Is Is Is Is --INFLAMMATION, GRANULONATOUS -) Is 14 Is Is Is Is 3), 0 1 0 0 0 0 TL) 15 Is is Is Is Is PROSTATE --INFL MMA H ..................N.U.MB.ER EXAMINED: 15 15 Is Is Is is Is Is 13 14 14 14 2) 0 0 2 0 0 1 3) 0 0 0 1 1 0 TL). IS Is Is Is Is is SEMINAL VESICLE ISVI ... . .....................NUMBER EXAMINED: IS Is Is Is Is is INCIDENCE TABLE 32 OF SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA, INC, MADISON, WISCONSIN U.S.A. 14 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GIIOUP-ALL-SCREEN-ALL;WEEKS-ALL DEATH-T;FIND-ALL;tUBSET-ALL N U M 8 t R - 0 F - A N IM A L S SIX: -----------M-ALE -----------GROUP: -I- -2- -3- -4- -S- -$- ORGA"/TISSUE EXAMINED NUMBER: IS is is is is is ---------------------------------------------------------------------------- --- --- --- -*- COAGULATING OLD (COI@ ............................N.U.MB.E.R . EXAMINED: 15 15 Is is is Is URETHRA (URI .....................................NU.M.BE.R EXAMINED: 15 Is Is Is Is Is KIDNEVIS ) ....................N.UM.BE.R EXAMINED: 0 0 0 1 1 a P), 0 0 0 1 1 0 TL) 0 0 0 1 1 0 SKIN FSKI .....................................NU.M.BE.R...EX.AMINED: 0 0 00 0 0 --DERMATITIS TL)@ 0 0 00 0 0 --ULCERA710H 7L). 0 0 00 0 0 SILEE!Cyllll .............................NU.M.BE.R.. EXAMINED: 0 0 00 0 0 - T. 'siibiA@'' YLi- 0 0 0 0 0 0 MAND104CEYlPtEVPRLNLSAIIMNI .............................NU.M.BE.R.. EXAMINED: LYMPHORETICULAR CELL 3). 1 1 0 0 0 0 00 1 1 0 0 TLi, 1 0 0 0 1 0 MESENTERIC LM INS) ....................... .....NUMBER EXAMINED: 0 0 0 0 0 0 LU NBAR/ILIAC LN ILM) ..................... ....NUMBER EXAMINED: 0 0 0 0 0 0 --HYPERPLASIA. LYMPHORETICULAR CELL TLJ, 0 0 0 0 0 0 END OF LIST INCIDENCE TABLE 33 OF SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES . AMIERICA. INC. MADISON. WISCONSIN U S.A. 22 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;G OUP-ALL-SCREEN-ALL;WEEKS-ALL DEATH-U;FINR D-ALL;iUB SIT-ALL --- N U H 8 E R - 0 F - A N I M A L S SEX: -----------M-ALE ------------ GROUP: -I- -2- -3- -4- -5- -6ORGAN/TISSUE EXAMINED ;;-T-O-P-O-F-L-I-ST---40---------------------------------------------NU-M-BE-R-: -----10----10 10 to 10 0 BRAIN IBRI .......:..............................N.UM.B.ER.. EXAMINED: 10 10 0 10 10 0 LUNG ILU) .....................................N.UM.B.ER...E.XAMINED: 10 10 10 10 10 0 LIVER ILI) ....................................N.U.MB.E.R ..EX.AMINED: --INFILTRATES.MONONUCLEAR CELL 10 10 to 10 10 0 - ]@ 1 0 1 9 7 0 l@ 1 3 1 1 1 0 2@ 1 1 1 0 2 0 TLD 10 10 10 10 to 0 --INFLAMMATION,GRAMULOMATOUS I to 10 10 10 0 1 2)@ 1 0 0 0 0 0 0 0 0 0 0 TL@ 10 10 10 to 10 0 --NECROSIS,COAGULATIVE --FATTY CHANGE CONTINUED ON NEXT PAGE 9 9 9 9 10 0 2) 1 1 1 1 0 0 TL@ 10 10 10 10 10 0 -) 10 10 9 10 10 0 3) 0 0 1 0 0 0 TL> 10 10 10 10 10 0 INCIDENCE TABLE 33 OF SEVERITY Of MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. 22 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;SCREEN-ALL;WEEKS-ALL DEATH-U;FIND-ALL;SUBSET-ALL N U M 13E R - 0 F - A N I M A L S SEX: -----------M-ALE -----------(IROUP: -I- -2- -3- -4- -S- -6- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 to 0 ------------------------------------------------------------------a----------- --- --- - 44 FROM PREVIOUS PAGE All LIVER (LI ........NUMBER EXAMINED: 10 10 10 10 to 0 BY AN ACCENTUATED -) 10 10 10 10 to 0 CENTRILOBULATED PATTERN IN WHICH THE NEPA70CYIES APPEAR 70 HAV TL)@ 10 10 10 10 10 0 A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE ROUNDED GIVING THE CELLS A MORE *PLUMP" APPEARANCE. EXCEPT FOR THIS *ENLARGED" APPEARANCE. THE CELLS ARE OTHERWISE "NORMAL". --"YPERPLASIA.BILE DUCT -P 10 9 10 to 10 0 2) 0 1 0 0 0 0 TL) 10 10 la 10 to 0 LF. TESTIS (TEO) ...............................N.U.MB.E.R . EXAMINED: 10 to 10 10 10 0 --ATROPHY/DEGENERATION 10 9 9 9 10 0 2@ 0 1 0 0 0 0 3) 0 0 1 1 0 0 TL@ 10 10 10 10 10 0 --INFLAMMATION, GRANULONATOUS -)- 10 10 10 10 10 0 TL) 10 to 10 10 10 0 PROSTATE --INFLIP=R Ti6h...6hi6N.iA@@fivi.. ...........N.UMBER EXAMINED: 10 10 10 10 10 0 1 10 10 10 1 0 2@ 2 0 0 0 1 0 5) 0 0 0 0 1 a TL), 10 10 10 10 10 0 co INCIDENCE TABLE 33 OF SEVERITY OF MICROSCOPIC OBSERVATIONS HAZLETON LABORATORIES . AMERICA, INC. MADISON, WISCONSIN U S.A. 22 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL-SCREEN-ALL:WEEKS-ALL DEATH-U;FIND-ALL;iUBSET-ALL U N 8 E R 0 F A N IM A L S SEX: -----------M-ALE ------------ OROUP: -I- -2- -3- -4- -5- -6- O-R-G-AN-/-TI-S-S-UE----EX-A-M-IN-E-D----------------------------------------N-U-MB-E-R:------1-0-----10-- 10 10 10 0 --- --- --- --- --- SEMINAL VESICLE (SV) ............................N.UM.B.ER.. EXAMINED: 10 10 10 10 10 0 COAGULATING OLD (CO- I ............................N.UM.BE.R.. EXAMINED: 10 10 10 10 10 0 URETHRA IUR) ..................................N.U.MB.E.R.-E.X.AMINED: 10 10 10 10 10 0 KIDNEY S) IDAN.O,.@i6 ...........................N.U.MB.E.R . EXAMINED: --IILATITI . L 0 0 0 1 0 0 P) 0 0 0 1 0 0 TL) 0 0 0 1 0 0 SKIN ISKI .....................................NU.M.BE.R...EX.AMINED: --DERMATITIS 1 0 0 0 0 0 S) 1 0 0 0 a 0 TL) 1 0 0 0 0 0 --ULCERATION S) 1 0 00 0 0 TL) 1 0 00 00 SPLEEN ISPI ...................................N.U.MB.E.R ..EX.AMINED: 1 0 0 0 0 0 --CY T. SEROSAL P) 1 0 0 0 0 0 TL) 1 0 0 0 0 a KAW0104CEY:PVPRLNLSAIIHN) LYM.PH.O.RE.T.IC.U.LA.N....CE.L.L ........ .....NUMBER EXAMINED:3) 00 00 00 00 11 00 TL) 0 0 0 0 1 0 14ESENTERIC LM (MS) ....................... .....NUMBER EXAMINED: 0 0 0 0 0 0 IIWI6329 Table 34 Summary of liomone Data Week 5 Mean so N 8 Mean SD N 14 Mean so N 22 Mean so N 0 20.1 1 22.6 1 b b T-5180 (Dym] 1 -10 30 Estradiol (ogImL) 21.0 b 0.42 2 - 26.8 4.56 4 b 34.2 29.1 - 6.99 6 b 20.9 26.7 1.16 4.17 3 2 28'.9 5.23 3 25.3 3.70 5 25.3 7.10 3 too 33.7 9.93 10 36.6 10.43 5 28.2 3.69 4 25.9 2.22 3 Testoster ne (nq/mL) 5 Mean 72,400 66,222 59,667 49,200 31,600 42 so 55,580.2 84,812.7 31,815.9 23,403.7 23,735.6 32 N 10 9 9 10 10 8 Mean 46,562 49,913 35,984 73,790 55,680 34 SD 39,398.1 66,500.6 22,768.7 103,947.1 35,655.4 38 N 10 10 10 10 10 a Animal were pair-fed with thi high-dose animals throughout treatment. b Mean is less than 20.0 pg/mL. HWI 6329- Table 34 (Continued) Summary of Hormone Oata Week 0 T-5180 (Dom) 10 30 io-O Testosterone (nq/mLl 14 Mean 24,578 35,649 31,478 23,577 20,413 19,4 so 12,656.3 27,614.6 21,909.0 19,074.1 14,625.6 16.0 N 10 10 10 10 10 22 Mean SD N 8,714 2,517.6 5 21,680 5,669.8 5 11,946 7,885.8 5 13,660 5,981.4 5 35,864 38,519.5 5 Mean SD N 8 Mean so N 14 Mean so N 22 Mean so N 0.5 0.19 10 0.5 0.12 9 0.4 0.07 9 0.4 0.17 5 Luteinizing(nq/mL) o.7 0.28 10 0.6 0.16 10 0.6 0.19 10 o.6 0.5 0.5 0.16 0.09 0.14 9 8 9 0.5 0.10 10 0.4 0.14 to 0.5 0.19 9 0.4 o.o5 4 0.5 0.12 5 0.5 0.32 5 0.5 0.12 8 0.5 0.12 10 o.6 0.45 10 o.6 0.22 4 a Animals were pair-fedwith the high-dose animals throughout treatment. HWI 6329-100 Table 35 Sumary of 'TestMaterial Residue Analysis Males -T-5180 (ppm) Grout) Week 4 6 5 Mean a SD N 6.5 1.05 8 55.4 8.08 9 103.5 159.3 a 14.38 30.16 a 8 10 a 8 Mean a 7.5 45.5 87.3 149.1 a so a 1.27 16.20 27.48 34.98 a N a 9 10 10 10 a 14 Mean so N a 7.1 41.2 a 1.15 12.98 a 10 10 70.3 137.6 a 16.18 33.83 a 10 10 a 22 Mean a SD a N a 1.2 1.09 1.303 3 1.64 0.918 3 2.45 0.849 2 a All values were below the limit of detection. 122 HWI 6329-ioo Table 36 Resultsof Method Validationfor Determinationof T-5180 in Rodent Food Expressed as the Percent of Recovery Theoretical Level (E)pm) 1 1 1 10 10 10 30 30 30 100 100 100 Analytical Assay Results (RDM) 0.810 1.08 0.760 DLY-1 Mean SD cv N 10.4 9.92 9.20 Mean 'SD cv N 30.1 34.2 30.6 Mean SD cv N 99.4 95.0 102 Mean SD cv (%) N Percent Theoretical 81.0 108 76.0 88.3 17.20 19.50 3 104 99.2 92.0 98.4 6.00 6.10 3 100 114 102 105 7.50 7.10 3 99.4 95.0 102 98.8 3.50 3.60 3 123 HWI 6329-100 Table 36 (Continued) Results of Method Validation for Determination of T-5180 in Rodent Food Expressed as the Percent of Recovery (Continued) Theoretical Level (pt)m) 1,000 1,000 1,000 Analytical Assay Results- (ppm) Day I (Continued) 977 972 1,072 Mean SD cv N Percent Theoretical 97.7 97.2 107 101 5.60 5.60 3 Day 2 1 -0.082 1 0.627 1 0.924 is 1.24 is 0.995 Ia 1.20 62.7 92.4 124 99.5 120 Mean 99.7 SD 24.60 cv 24.7 N 5 10 10.6 10 9.85 10 9.66 106 98.5 96.6 Mean 100 SD 5.00 cv 5.00 N 3 a Data are from reanalysis of sample. 124 HWI 6329-100 Table 36 (Continued) Results of Method Validation for Determination of T-5180 in Rodent Food Expressed as the Percent of Recovery (Continued) Theoretical Level (DVM) 30 30 30 100 100 100 1,000 1,000 1,000 Analytical Assay Results- (DRM) Day 2 (Continued) 28.8 28.0 28.3 Mean SD cv N 97.1 93.3 94.3 Mean SD cv N 1,006 955 940 Mean SD cv N Percent Theoretical (%I 96.0 93.3 94.3 94.6 1.30 1.40 3 97.1 93.3 94.3 94.9 2.00 2.10 3 101 95.5 94.0 96.8 3.70 3.80 3 125 IIWI6329 Table 37 Results of StabilityAnalyses Storage Conditions Day of mixing, 12119190 Replicate 0 I - 2 - Mean - 1.27 1.39 1.33 (133)' T-5180 ippm) 10 10.0 8.90 9.45 (94.5) 30 28.3 27.7 28.0 (93.3) Refrigerated,I day; I room temperature, 2 7 days Mean - 0.81 11.8 28.9 - 0.75 10.8 33.7 - 0.78 (78.0) 11.3 (113) 31.3 (104) Frozen, 2 wee@ks I - 0.75 2 - 0.78 10.2 12.2 28.7 29.5 Mean - 0.77 (77.0) 11.2 (112) 29.1 (97.0) Frozen, 7 weeks I - 1.20 2 - 1.47 11.1 9.44 26.6 26.6 mean - 1.34 (134) 10.3 (103) 26.6 (86.7) a Values in parenthesesare percent of theoretical. IIW1 6 Table 37 (Continued) Results of StabilityAnalyses Storage @onditione Day of mixing, 03/06/91 Room temperature, 8 days Replicate 0 1 T-5180 (1)1)m) 10 30 I - 0.68 9.09 28.9 2 0.76 9.62 29.7 Mean - 0.72 (72.0)' 9.36 (93.6) 29.3 (97.7) I - 0.74 9.40 29.5 2 - 0.76 9.72 29.7 Mean - 0.75 (75.0) 9.56 (95.6) 29.6 (98.7) a Storage'conditionreflect actual conditionsthat samples were stored from Weeks I through 8. b Values in parenthesesare percent of theoretical. IIWI 6329-100 Table 38 Results of Homogeneity Analyses Sample Location Replicate 0 1 T-5180 (ppm) 10- 30 105 Top I - 0.72 (72.0)' 11.6 (116) 31.3 (104) 104 2 - 0.65 (65.0) 12.0 (120) 29.8 (99.3) 106 Left I - 0.95 (95.0) 10.7 (107) 29.4 (98.0) 114 2 - 0.88 (88.0) 10.9 (109) 31.1 (104) 119 Right I - 1.21 (121) 12.8 (128) 34.2 (114) 107 2 - 1.54 (154) 11.6 (116) 30.5 (102) 106 Bottom I - 0.85 (85.0) 11.9 (119) 28.7 (95.6) 106 2 - 0.84 (84.0) 12.7 (127) 31.0 (103) 110 a Values in parentheses are percent of theoretical. OD HWI 6329-100 Table 38 (Continued) Results of Homogeneity Analyses* Sample -T-5180 (ppm) Location Replicale 0 1 10 .30 100 Top I - 0.84 (84 O)b - - - 2 - 0.88 (88.0) - - - Left I - 0.88 (88.0) - - - 2 - 0.86 (86.0) - - - Right I - 0.73 (73.0)' - - - 2 - 0.74 (74.0)' - - - Bottom I - 0.71 (71.0) - - - 2 - 0.87 (87.0) - - a Reanalyzed,01/07/91. b Values in parentheses are percent of theoretical. c Results are from a reanalysison 1/15/91. to IIWI6329-100 Table 39 Results of Dose Preparation Analyses gglek Repligatt 0 T-5180 (Dom) 10- 30 I I - 0.80 (80.0)- 11.6 (116) 28.9 (96.3) 2 - 1.19 (119) 11.4 (114) 29.5 (98.3) 2 1 - 0.80 (80.0) 9.73 (97.3) 32.2 (107) 2 - 0.91 (91.0) 10.8 (108) 33.0 (110) 3 1 - 0.91 (91.0) 10.1 (101) 34.9 (116) 2 - 0.74 (74.0) 11.5 (115) 31.4 (105) 4 1 2 4b 1 2 - 0.73 (73.0) 11.5 (115) 30.4 (101) - 0.74 (74.0) 9.88 (98.8) 30.6 (102) 0.88 (88.0) - - 0.82 (82.0)' - - 5 1 - 0.86 (86.0) - - 2 - 0.88 (88.0) - - 5b I - 1.10 (110) - - 2 - 1.17 (117) - - 6 1 - - 2 - 9.58 (95.8) - 10.4 (104) - a Values in parenthesesare percent of theoretical. b Archived sample. 100 111 (11 102 (10 100 (10 108 (10 98.4 ( 110 (11 98.0 102 (10 - - IIWI 6329-100 Table 39 (Continued) Results of Dose Preparation Analyses Week Replicate 0 1 7 1 - 2 - T-5180 (opm) 10 - 30 100 29.1 (97.0)' - 26.1 (87.0) - 8 1 - 2 - - - 97.3 96.6 9 1 - 0.70 (70.0) - 2 - 0.75 (75.0) - 10 1 - - 2 - - 10.3 (103) 9.01 (90.1) - 11 1 - - 2 - - - 27.7 (92.3) - 28.8 (96.0) - 12 1 - - 2 - - - - 90.1 - - 93.2 13 1 - 0.76 (76.0) - - - 2 - 0.72 (72.0) - - - 14 1 - - 9.34 (93.4) - - 2 - 9.45 (94,S) - - a Values in parenthesesare percent of theoretical. HWI 6329-100 APPENDIX A Protocol Deviations Protocol TP9321 Protocol Amendment No. I Protocol Amendment No. 2 Protocol Amendment No. 3 Material Safety Data Sheet 132 HWI 6329-100 Protocol Deviations Protocol Dose Preparation. "All dose preparations will be refrigerateduntil administration." Dose Analyses. "Four additional sets of samples will be taken from each dose preparation mixed for Week I...One set will be kept for at least the same period and under the same conditions that are expected to be used for the study, then analyzed." Special Analysis. Test Material Residue Analysis. "An aliquotof serum ...will be collected and stored until analysis for test material residue.' Special Analysis. Disposition. "Serum samples will be stored at approximately -70'C until shipment." Termination. "Animalswill be anesthetized with methoxyflurane, exsanguinated, and necropsied between 7:00 a.m. and 10:00 a.m." Gross Necropsy. Cut surfaces of the brain and spinal cord will be examined when tissues are trimmed. Actual Procedure During Weeks 1 through 8, diets were stored at room temperature until administration. None of the sets of samples mixed for Week I were stored under the same conditions as was used for Weeks I through 8, so a second stabilityanalysis was done at Week 12. All serum samples from the Week 22 collectionwere sent to Hazleton Washington for hormone analysis. The serum remaining after hormone analysis was returned to Hazleton Wisconsin for test material residue analysis. Due to an insufficient amount of serum remaining after hormone analysis, some groups had serum from less than 10 animals available for test material residue analysis. The location of serum sample storage for 3 collectiondates was not documented and cannot be verified. Two animals were not weighed before exsanguination. Three animals were necropsied after 10:00 a.m. The spinal card was not collected at necropsy; therefore, cut surfacesof the spinal cord were not examined when tissues were trimmed. These deviations are not expected to have affected the results of the study. 133 HAZLETON W IS C 0 N S IN 3201 OLTNSOAAN SOULEVAPTO OST Off,CE SOX IS&S 07 .IAD SON WTSCONSIN S37 CKW44000 Sponsor: 3M St. Paul, Minnesota PROTOCOL TP9321 St@,Ce$ CO Ca-. Study Title: 13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate(CAS No. 3825-26-1) in Male Rats November 30, 1990 Performing Laboratory: Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704 LaboratoryProiect Identification: HWI 6329-100 u 0 d s 18 10 & - G 0 aMv . j60an F ra 0 .U P 6 0 8 -2 A I A A 7 1 F 6 0 0 2 4 1 7 2 2 7 't0 e 0 3 9 5 6 04 A Z 91 A N' 0 S 134 HWI 6329-100 TP9321 Page 2 STUDY IDENTIFICATION 13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate(CAS No. 3825-26-1) in Male Rats Test Material Sponsor Study Monitor Study Director Study Location Proposed Study Timetable In-Life (Experimental)Start Date In-Life Termination Date Experimental Termination Date T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000 Roger G. Perkins, PhD, DAST 3M Toxicology Services Building 220-2E-02, 3M'Center St. Paul, Minnesota 55144-1000 Matthew J. Palazzolo, PhD, DABT Hazleton Wisconsin, Inc. P.O. Box 7545 Madison, Wisconsin $3707 (608) 242-2622 Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704 December 20, 1990 May 16, 1991 To be provided by amendment 135 1. Study 2. Purposes 3. Regulatory Compliance 4. Quality Assurance S. Test Material A. Identification S. Purity C. Stability 0. Storage Conditions E. Characteristics F. Reserve Samples G. Safety Precautions WWI 6329-100 TP9321 Page 3 13-Week Dietary Toxicity Study with T5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats To assess the subchronic toxicity of the test material when fed to rats for at least 13 weeks and to evaluate reversibilityof any toxic effects This study will be conducted in accordance with the Environmental Protection Agency Good Laboratory Practice Standards, 40 CFR 160. The protocol, study conduct, and final reportwill be audited by the Quality Assurance Unit in accordance with Hazleton Wisconsin, Inc. (HWI) Standard Operating Procedures (SOPs). T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) Jo be determined by the Sponsor To be determined by the Sponsor In a dry area, at room temperature Information on synthesis methods, composition,or other characteristics that define the test material is on file with the Sponsor. A reserve sample of each lot will be taken and stored in a freezer. These sampleswill be transferredto the Sponsor after completion of the inlife phase of the study to be retained in accordance with 40 CFR 160.195. Any remainingtest material will be returnedto the Sponsor after their authorization. As required by HWI SOPS, unless otherwise specified 136 HWI 6329-100 TP9321 Page 4 6. ExperimentalDesign A. Animals (1) Species (2) Strain/Source (3) Age at Initiation of Treatment (4) Weight at Initiation of Treatment (5) Number and Sex (6) Identification (7) Husbandry (a) Housing (b) Food (c) Water (d) Contaminants (e) Environment (f) Acclimation Rat Crl:CDOBR/Charles River Laboratories, Inc. Preferably4 to 6 weeks, but not older than 8 weeks Approximately 75 to 250 g 320 males Individually numbered ear tags Individual Certified Rodent Chow #SO02 (Purina Mills, Inc.) ad libitum, unless otherwise specified. The food is routinely analyzed by the manufacturer for nutritional components and environmental contaminants. Ad libitum. Samplesof the water are analyzed by HWI for total dissolved solids, hardness, and specified microbiologicalcontent and for selected elements, heavy metals, organophosphates, and chlorinated hydrocarbons. There are no known contaminants in the food or water that would interferewith this study. Environmental controls for the animal room will be set to maintain a temperatureof 72*F 6*, a relative humidity.of 50% +20%, and a 12-hour light/12hour da7k cycle. At least I week 137 HWI 6329-100 TP9321 Page 5 (8) Randomization Via computer-generated random numbers for assignment to groups. The animals will be weighed. The weight variation of the animals selected for the study will not exceed 2 standard deviations of the mean weight, and the mean body weights for each group will not be statisticalldyifferentat the 5% probabilitylevel. (9) Justification The rat is frequentlyused in safety evaluation studies as a representative of a rodent species. Male rats are used to characterizethe effects of the test material on testicular physiology. B. Group Designations Dose Level (I)DM) Number of Males' 0 55 2 1 55 3 10 55 4 30 55 5 1.00Ob.. 55 6 45 a 10 animals for Groups I through 5 (designatedas recoveryanimals)will be treatedfor at least 13 weeks, then treatment will be discontinuedand they will be observedfor reversibility, persistence,or delayed occurrenceof toxic effects for at least 8 weeks posttreatment. b Animals in Group 6 will be pair-fed with the high-dose animals (Group 5) throughout the treatmentperiod. Due to the pair-feedingrequirements,the pair-fed controlanimals (Group 6) will initiateon test 1 day after all other groups. C. Dosing Procedures (1) Dosing Route Dietary (2) Reason for Dosing Route To compare the test data with other studiesdosed in a similar fashion. (3) Dosing Duration At least 13 weeks 138 HWI 6329-100 TP9321 Page 6 (4) Dose Preparation (5) Dose Analyses (a) Homogeneity (b) Stability (c) RoutineAnalyses 0. Observationof Animals (1) CagesideObservations (2) Antemortem Observations Weekly. All diets will be mixed according to the mixing procedure. Dose concentrationswill be based on the test material as supplied. All dose preparationswill be refrigerated until being dispensed into food containers. By NWI. Additional samplesmay be assayed at the discretion of the Study Director. One sample each from all dose levels mixed for Week 1 will be taken from the top, bottom, and two opposing sides of the dose preparation and assayed for test material content. All samples will be stored in a freezeruntil analyzed. Four additional sets of samples will be taken from each dose preparation mixed for Week 1. One set will be analyzed on the day of mixing. One set will be kept for at least the same period of time and under the same conditions that are expected to be used for the study, then analyzed. The remaining two sets will be stored in a freezer and assayed after 2 and 7 weeks. All dose preparationswill be assayed each week for the first 4 weeks. Each week thereafter. one test material dose concentrationwill be selected sequentially. Samples from each 4week period will be analyzed as a group with a sample of the control diet. Mortality and moribunditycheck twice daily (a.m. and p.m.). Additional signs of poor health or abnormal behaviorwill be recorded as they are observed. At least once weekly, each animal will be removed from its cage and examined. 139 (3) Body Weights (4) Food Consumptions E. Special Analysis (1) Hormone Analysis (a) Methodof Collection (b) Disposition NWI 6329-100 TP9321 Page 7 Before initiationof treatment,on the first day of treatment,weekly thereafter, and on the day of necropsy Daily for the animalsthat will be pair-fed (Groups 5 and 6); weekly for all other animals. During recovery, Group S animals will be fed weekly. Serum samples will be collected from animals scheduled for sacrificeat each intervalfor analysisof estradiol, testosterone(total),and luteinizing hormone. Serum samples from 10 animals/group selected at random at each scheduled sacrificewill be analyzed from each sacrificeinterval during treatment. Serum samples from 5 animals/groupwill be analyzed at recovery. The followingmethodswill be used for the hormone analysis: Lu.teinizinghormone s radioimmunoassay E tradiol - DiagnosticProductsKit (DPCKE201) Testosterone - Diagnostic Products Kit (OPCTKTTZ) Nonfasted animals will be anesthetized with methoxyflurane;blood will be collected from the descending vena cavi and stored on ice (approximately4*C) until centrifugation. Serum samples will be stored at approximately -70*C until shipment. All samples will be shippedon dry ice to: Denise Novello Immunochemistry Division Client Services Section Hazleton Washington 9200 Leesburg Turnpike Vienna, Virginia 22182 140 HWI 6329-100 TP9321 Page 8 (2) TestMaterial Residue Analysis (3) HepaticPalmitoyl CoA Oxidase F. Termination (1) UnscheduledSacrifices and Deaths (2) ScheduledSacrifice G. Postmortem Procedures (1) Gross Necropsy Samples of liver, testes, lungs, and subcutaneous adipose from each animal at necropsywill be collectedand stored frozen for possible analysisfor testmaterial residue (at an additional cost to the Sponsor; method to be providedby the Sponsor). An aliquot of serum (see Section 6. E. 1. Hormone Analysis)will be collectedand stored for possible analysis for test material residue. A sectionof liver will be obtained from all animals at each scheduled sacrificeand will be flash frozen using liquid nitrogen. A sectionof liver from 5 animals/group,selectedat random at each scheduled sacrificewill be assayed for the level of palmitoyl CoA oxidase as an indicator of peroxisome proliferation. Necropsieswill be done on all animals that die or are sacrificedin a moribund condition. After at least 4, 7, and 13 weeks of treatment,animals (15/group)will be anesthetized with methoxyflurane, weighed, exsanguinated, and necropsied between 7:00 a.m. and 10:00 a.m. For recoveryanimals (10/group),thiswill be done after at least 13 weeks of treatment and at least 8 weeks without treatment. The necropsy will include an examination of: External surface of the body All orifices Cranial cavity External surfaces of the brain and spinalcord (Cut surfaceswill be examined when tissues are trimmed.) 141 HWI 6329-100 TP9321 Page 9 (2) OrganWeights (3) SpecialAnalyses (4) TissuePreservation Nasal cavity and paranasalsinuses Thoracic,abdominal,and pelvic cavitiesand viscera For each animal at scheduledsacrifice, the followingorgans will be weighed; paired organs will be weighed separately: Brain Liver Lungs Testes Accessory sex organs (seminal vesicle,prostate,coagulating gland,urethra) Organ-to-bodyweight percentages and organ-to-braiwneight ratioswill be calculated. Samplesof liver,testes, lungs,and subcutaneousadiposewill be collected from each animal for possible analysis for testmaterialresidue. An additionalsectionof liverwill be 'collectedfrom each animal for possible analysisof PalmitoylCoA. (See Section6. E. 3. Hepatic PalmitoylCoA Oxidase). The followingor representativesamples thereof,will be collected and preserved in 10% phosphate-buffered formalin,unless otherwisespecified. Samples of these tissueswill be preservedin glutaraldehydefor further processingif electronmicroscopic evaluationis desired. Lesions Brain Liver Lungs Testes Accessory sex organs (seminal vesicle,prostate,coagulating gland,urethra) 142 NWI 6329-100 TP9321 Page 10 (5) Histopathology 7. Reports. A. FinalReport (1) ExperimentalDesign and Methods (2) Results (3) StatisticaAlnalyses The above (as appropriate)will be embedded in paraffin,sectioned, stainedwith hematoxylinand eosin, and examinedmicroscopicallyfrom all animalsfrom which they are collected A finalreportincluding,but not limitedto, those itemslistedbelow will be submitted. One copy of the draft report and two copies of the finalreportwill be provided. As definedby the protocol,protocol amendments,and any protocol deviations Mortality Antemortem observations Body weights Cumulative body weight gains Food consumption Compound consumption Palmitoyl CoA Organ weights Orga.n-to-bodyweight percentages Orgah-to-brainweight ratios Macroscopic observations Microscopic observations Body weights Cumulative body weight gains Food consumption Organ weights Organ-to-bodyweight percentages Organ-to-brainweight ratios Palmitoyl CoA Hormone Analysis Groups 2, 3, 4, and 5 will be compared with Group 1; Group 6 will be compared with Group S. Statisticalmethodswill be those used by HWI (Attachment1). 143 HWI 6329-100 TP9321 Page II (4) Data to be reported (5) AnalyticalChemistry 8. Maintenanceof Raw Data, Records, and Specimens Incidenceof antemortem observations Mean body weights Mean cumulativebody weight gains Mean food consumptions Mean compound consumptions Mean group hormone values Mean Palmitoyl CoA Mean organ weights Mean organ-to-bodyweight percentages Mean organ-to-brainweight ratios Incidenceof macroscopic observations Incidenceof microscopicobservations Individualantemortemobservations(to includefate and date of death for each animal) Individualbody weights Individualfood consumptions IndividualPalmitoylCoA values Individualorgan weights Individualorgan-to-bodyweight percentages Individualorgan-to-brainweight ratios Individualmacroscopicobservations Individualmicroscopicobservations Methods and results (if done by HWI) Originaldata, or copiesthereof,wi,il be availableat HWI to facilitate auditingthe study during its progress and before acceptanceof the final report.'When the finalreportis completed,all originalpaperdata, all magneticallyencoded records, and a copyof the final reportwill be retainedin the archivesof HWI. All specimenswill be transferredto the Sponsor to be retained in accordance with 40 CFR 160.195. 144 PROTOCOL APPROVAL NWI 6329-100 TP9321 page 12 R00994**rrrG6*PPeqrekLins, PhD, DAST Study Monito 3M a thew J. Pa Zola, Study Director zolo@, Toxicology , UBT HazletonWisconsin, Inc. regperosseelnnt-aettI@-vf@a0-t QualityAssuranceUnit HazletonmisconsinlInc. Da RECEIVED DEC 0 6 in HLA-Madison 145 HWI 6329-100 TP9321 Page 13 Attachment I StatisticalAnalyses The statisticalmethods that will be used are described below. Levene'stest (Levene,1960) will be done to test for variancehomogeneity. In the case of heterogeneityof varianceat p 1 0.05, transformationswill be used to stabilize the variance. Analysis of variance (ANOVA) (Winer, 1971) will be done on the homogeneousor transformeddata. If the ANOVA is significant,Games and Howell Modified TukeyKramer test (Games and Howell, 1976) will be used for pairwise comparisons between groups. One-way ANOVA will be used (if applicable)to analyze body weights; cumulative body weight gains; food consumptions; Palmitoyl CoA and Hormone Analysis; organ weights; organ-to-bodyweight percentages;and organ-to-brainweight ratios. If the ANOVA shows significance for body weights at Week 0, one-way analysis of covariance (ANCOVA) (Winer, 1971) will be used to analyze body weights, with the initial body weight as the covariate. AlthoughLevene's test for variance homogeneitywill be done (see above), no transformationswill be used because covariance adjustment removes extraneous heterogeneity. If the ANCOVA is significant, Games and Howell Modified Tukey-Kramer test will be used for pairwise comparisons between groups. Group comparisonswill be evaluatedat the 5.0% two-tailed probabilitylevel. References Games, P. A., and Howell, J. F., *PairwiseMultiple Comparison Procedureswith Unequal N's and/or Variances: A Monte Carlo Study,* Jgurnal of Educational Statistics,1(2):113-125(1976). Levene, M., "RobustTests for Equalityof Variances,* @ontributionsto Probabilityand Statistics,(eds.)1. Olkin et &I., Ch. 25, pp. 278-292, StanfordUniversityPress: Stanford,California(1960). Winer, B. J., "Analysisof Covariance,"StatisticalPrinciples in Experimental Design, Second Ed., Ch. 10, pp. 752-812, McGraw-Hill: New York, New York (1971). Winer, B. J., "Design and Analysis of Single-FactorExperiments,n Statistical Principles in ExperimentalDesign, Second Ed., Ch. 3. pp. 149-260, McGraw-Hill: New York, New York (1971). 146 ki.ll,pHAZLETON W IS C 0 N S IN ':3i!_c iLli.E -LAO cs F s 50 s 53 7c HWI 6329-100 PROTOCOL TP9321 13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats HWI 6329-100 Sponso 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, MN 55144-1000 Study Monitor Roger G. Perkins, PhD, DABT Contractor Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, Wi 53704 Study Director Matthew J. Palazzolo, PhD, DABT Amendment NO. I.- This amendment modifies the following portions of the protocol: Effective December 10, 1990 1. Page 6. 6. ExperimentalDesign, C. Dosing Procedures, (4) Dose Preparation,Sentence3. To clarify the actual procedure, delete this sentence and replace with the following: All dose preparationswill be refrigerateduntil administration. e .1 S e 60 6 Z4 Ge 4A 71 60a 2 22 7 703956 2 147 Amendment No. 2 Page 2 HWI 6329-100 2. Page 6. 6. Experimental Design, C. Dosing Procedures, (5) Dose Analyses. (c) Routine Analysil. To clarify the actual procedure, delete this sectionand replacewiththe following: All dose preparations will be assayed each week for the first 4 weeks. Each week thereafter, the control diet and one test material dose concentration (selected sequentially) will be collected. Samples from each 4-week period will be analyzed as a group. 3. Page 9. 6. Experimental Design, G. Postmortem Procedures, (3) Special Analyses. To clarify the actual procedure, add the underlined as follows: (3) Special Analyses tscheduled sacrifice animals only) 4. Page 9. 6. Experimental Design, G. Postmortem Procedures, (4) Tissue Preservation, Sentence 2. To clarify the protocol, add the underlined as follows: Samples of these tissues (scheduled sacrifice animals only) will be preserved in glutaraldehyde for further processing if electron microscopic evaluation is desired. Effective December 31, 1990 5. Page 6. 6. Experimental Design, C. Dosing Procedures, (5) Dose Analyses. To include the collection of retention samples and a second homogeneity for Diet 2, delete this section and replace with the following: (5) Retention Samples Samples will be taken from the base diet (premix) and from each dose preparation and stored in a freezer for at least 6 months, unless used for analysis. At the discretion of the Study Director, these samples will be returned to the Sponsor or discarded. (6) Dose Analyses By HWI. Additional samples may be assayed at the discretion of the Study Director. (a) Homogeneity One sample each from all dose levels mixed for Week I will be taken from the top, bottom, and two opposing sides of the dose preparation and assayed for test material content. Due to a change in the mixing procedure for the I ppm level, one sample from the I ppm level mixed for Week 3 will also be taken from the top, bottom, and two opposing sides of the dose preparation and assayed for test material content. All samples will be stored in a freezer until 148 analyzed. Amendment No. 2 Page 3 HWI 6329-100 (b) Stability (c) RoutineAnalyses Four additionalsets of samples will be taken from each dose preparation mixed for Week 1. One set will be analyzed on the day of mixing. One set will be kept for at least the same period of time and under the same conditions that are expected to be used for the study, then analyzed. The remainingtwo sets will be stored in a freezer and assayed after 2 and 7 weeks. All dose preparations will be assayed each week for the first 4 weeks. Each week thereafter,the control diet and one test material dose concentration (selectedsequentially)will be collected. Samples fgom each 4-week period will be analyzed as a group. AMENDMENT APPROVAL Receiv--,, JAN 2 8 1"1 HoMeton Wisconsin Roger4. Perkins, PhD Diplomate, ABT Study Monitor 3M I//7/f/ Date Matt ew J. Pal zol WhD Diplomate, AST Study Director Toxicology Hazleton Wisconsin, Inc. Dati( Representat,TvO Date Quality Asur,ance Unit Hazleton Wisconsin Inc. (ADM/jle) 01/10/91 149 HAZLETON W IS C 0 N S IN 33 h i %A AN 9 0 '.JL EVA AD p c s 0 9 c Eco 1 0 x k4 A 0 SON YWIS NSIN S3707 HWI 6329-100 a ooft,4040 awgtav $*,.Cos Cc@-w. PROTOCOLTP9321 13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate(CAS No. 3825-26-1) in Male Rats NWI 6329-100 Sponsor 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, MN 55144-1000 Study Monitor Roger G. Perkins, PhD, DAST Contractor Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, Wl 53704 Study Director Matthew J. Palazzolo, PhD, DABT Amendment No. 2 Effective December 10, 1990 This amendment modifies the followingportion of the protocol: I. Page 9. 6. Experimental Design. G. Postmortem Procedures, (4) Tissue Preservation, and Amendment No. 1. Item 4. To clarify tissue collection for possible electron microscopicevaluation,delete this section and replace with the following: (4) Tissue Preservation The following or representative samples will be collected and preserved in 10% phosphate-buffered formalin, unless otherwise specified: Lesions Brain Liver Lungs Testes (one testis) Accessory sex organs (seminal vesicle, prostate, coagulating gland, and urethra) S G p: ISO 6 0 2 iLi -a A 7 i 502 2A 1 722 7 T 70 3 9 5 6 A Z IR A -V Amendment No. 2 Page 2 HWI 6329-100 Samples of the following tissues (scheduledsacrificeanimals only) will be preserved in glutaraldehyde for furtherprocessingif electron microscopicevaluation is desired: Brain Liver Lungs Testes (one testis) Accessory sex organs vesicle,prostate) (seminal AMENDMENT APPROVAL Roger OU Perkins, PhD Diplomate, ABT Study Monitor 3M Matthew J. Paaazzolo@ILthD Diplomate, ABT Study Director Toxicology Hazleton Wisconsin, Inc. @-epresentative Quality Assurance Unit Hazleton Wisconsin Inc. (ADM/jle) 01/21/91 129 Date Date Date ReceiVed JAN 3 11991 Hazleton Wisconsin 151 tiAZLrg@C(ON 'v@w I s c 0 N 5 1 N Ias 1 0 ofIcl Box ?Sit HWI 6329-100 PROTOCOL TP9321 13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate(CAS No. 3825-26-1) in Male Rats HWI 6329-100 Sponsor 3M Toxicology Services Building 220-2E-02.3M Center St. Paul, MN 55144-1000 Study Monitor Roger G. Perkins, PhD, DABT Contractor Hazleton Wisconsin,Inc. 3301 Kinsman Boulevard Madison, Wl 53704 Study Director Matthew J. Palazzolo, PhD, DABT Amendment No. 3 This amendmentmodifiesthe followingportionsof the protocol: Effective November30, 1990 I. Pace2. STUNY IDENTIFICATIONP.roposedStudyTimetable.Experimental TerminationDate. Because the finaldate of data collectionhas been determined,delete wTo be provided by amendment,and replace with the following: March 29, 1993 Effective April S. 1991 2. ace B. 6. perimentalDes an. E. Spacial Analysis(2) Test Material and Residue-Analysis,Sentence 2. At the Sponsor'srequestto analyzeserum for test materialresidue,delete this sentenceand replacewith the following: An aliquotof serum (see Section 6. E. I. HormoneAnalysis)will be collectedand storeduntil analysisfor testmaterialresidue. p ot 608 241 44" D, zz, 3 20 i "A -I 604 7.''' 152 Amendment No. 3 Page 2 AMENDMENT APPROVAL NWI 6329-100 Q4& koge-r Q Perkins, PhD Diplomate, AST Study Monitor 3M lo,lf Pal Olo, Oiplomate,ABWI Study Director Toxicology Hazleton Wisconsin, Inc. d. Representative Quality Assurance Unit Hazleton Wisconsin Inc. 3 Dati Date 1-1711,2 Date 153 3H General offices 3H Center St. Paulg MN 53144-1000 (612) 733-1110 buns No.t 00-617-3082 MATERIAL SAFETY DATA SHEET FACSIMILE COPY HWI-6329-100 DIVISION: INDUSTRIAL CHEMICAL PRODUCTS DIVISION TRADE NAMEs FC-143 FLUORAD Brand Fluaroch*oical Surf&ctant 3A I.D. NUMBERs 90-0211-0009-0 99-0211-0367-0 ZF-0002-0378-4 ISSUED: March 20, its? 90-0211-0991-9 SUPERSEDESs July 27, 1988 DOCUMENTs 10-3800-2 --------------------------------------------------------- - --------------------- :---- EXPOSURE LIMITS ---- I- INGREDIENTS CAS NO. PERCENT VALUE UNIT TYPE AUTN -------------------------------------------------------------------------------- Assonius Perfluarcalkyl Carboxyl&to Assonjus Perfluaro&lkyl Carbozyl&to Ammonius Perfluoro&lkyl C&rbozyl&to 3823-26-1 6130-43-4 21615-47-4 t5.0-100.0 0.1 < 5.0 *.1 < 5.0 0.1 oq/&3 TWA ACCIM oq/e3 TWA 3M "/&3 TWA 3n AO&Onius Perfluoro&lkyl Carboxyl&te "239-11-0 w < 5.0 0.1 "/&3 TWA 3m SOURCE OF EXPOSURE LIMIT DATAs ACGINS American Conference of Governomtal Industrial Hygienists 3RS 3M Exposure Guidelines -- ----- --- ---------------------- ------------ 2. PHYSICAL DATA -------- ----------- ---------------------------- BOILING POINTI .......*..*.....WA VAPOR PItESSUREs .............W.A. VAPORDENSITY.s........*...Ooo wa EVAPMTZON RATEs .........o.., WA SOLUBILITY IN WATERs...oo..ooo >10 % SPECIFIC GRAVITTs.o .... 0.3"-0.300 (Bulk) ------ - - ------ ----------------- PERCENT VOLATUK#.,* ... .....WA VOLATILEORGANIC$.#..........w.o PHI .........0*....0* ...0.000.0ca. 5.00 (0.3% Aquems) VISCOSITYI ............so....** WD APPEARANCE AM ODMI Light colored p"derl slight odor. 3. FIRE AM EXPLOSION HAZARD DATA -- ----------------------- -- FLASH Non-flaa"blo FLAMMABILITY LINIT - LELs..oos WA FLAMMABILITY LIMIT - UELs..... WA AUTOIGNITION TEMPERATUREs .....wv IEXTINGUISHINO FIEDIA& water, foas, COZ, P" Chosical 154 31vi MSOST FC-143 FLUORAD Brand FluarCChosical Surfactant Mar-20-19e9 PAGE 2 ------------------------------------------------------------------------------ HWI 6329-100 3. FIRE AND EXPLOSION HAZARD DATA (continued) ------------------------------------------------------------------------------ SPECIAL FIRE FIGHTING PROCEDURES: Non* Known UNUSUAL FIRE AND EXPLOSION MAZARDST Heat of fire may release ammonia and HF. -------------------------------------------------------------------------------- 4. REACTIVITY DATA -------------------------------------------------------------------------------- .STAOILITYI Stable INCOMPATABILITY - MATERIALS TO AVOIDT - NA HAZARDOUS HAZARDOUS POLYMERIZATIONS DECOMPOSITION Will Not Occur FIRODUC7Sj Thermal decomposition may produce toxic materials including W. -------------------------------------------------- m ----------m ------------------ 5. ENVIRONMEWTAL INFORMATION --------------M---------------------------------------------------- SPILL RESPONSES Observe precautions from other sections. Use toxic dust eask. Use met sweeping compound, or water to avoid dusting. Collect spilled material. Place in a closed container. RECOMMENDED DISPOSALS Mix with flammable material and incinerate in an Industrial or commercial facility. Combustion products will include W. Discharge spent solutions to a mastemater treatment system. Reduce discharge rate if tossing occurs. Since regulations va", consult applicable regulations or authorities before disposal. U.S. EPA Maiardous Vast@ Ne.1 None ENVIRONMENTAL DATA: Chemical Oxygen Demand (COO)I Nil (700 ag/gq)l 20-Day Biochemical Oxygen Demand (RODZO)I Will 96-Hr. LCSC, Blu"ill Sunfish (Lopoets macrachirus)s 569 eq/Li 96-Hr. LCSQ, Fathoad Hinnow(Pi"phalis proselas)e 766 sq/L; 4"r EC30, Daphnis sagnat 632 "/L; 14-Day ECSO (cell dry voight), Or*" Alga* (Solonastrus capricaroutus) 73 sq/L. SARA HAZARD CLASSI FIRE HAZARDS No PRESStMEs No ItEACTIVITYe No ACUTEs Yes CHRMC& Yes, --------------------- ---- --- - - ----------- ----- -------- -------- 6. SUGGESTED FIRST AID ---------------- EYE CONTACTS - ------- ---- - ------- Immediately flush with plenty of water. Continue a physician. SKIN CONTACTS for 10 slautes. Call Wash affected INHALATIONS area with soap and water. If symptoms occur, remove person to fresh air. If symptoms continue, call a physician. INGEST;ONT DO NOT IND= VOMITING. Give copious amounts of water. IMMIATELY call a physician or Poison Control Center. OTHER FIRST AID: -------------------------- ----- ---------------------- --- ---------- Abbrev:.ations: N/D - Not Determined Wi - Not Applicable 155 MSOS: FC-143 FLUORAD Brand Fluarochosical Surl&ctant 3M Mar-29-lt99 PAGE 3 ------------------------------------------------------------------------------ 6. SUGGESTED FIRST AID HWI 6329-100 --------------------------m-m --------------m-------------------m ----------- NOW ------------------------w ---------M ----------------------------------------- 7. PRECAUTIONARY IWGRMATION --------------------------------------------------------------mm----------- Use only in well ventilated areas with sufficient local exhaust ventilation to maintain airborne levels at recognized health and safety levels. Do hot breath* dust. Do not got powder or airborne dust in eyesl wear personal eye protection. Do not got on skini wear impervious gloves and protective clothing. V4sh hands thoroughly after use before eating or drinking. Do not take internally. Launder contaminated clothing before re-use. NOTEs FC-143 was fed to albino rats for 2 years; no compound Induced carcinogenicity was found in the study. There were statistically significant compound related benign testicular tumors. Based on current knowledge, the findings have no human health implications. *RXPREVENT MOISTURE CONTARIMTICN TO KEEP POWDER FREE FLOWING.*$* SPECIAL PROTECTIONI EYE PROTECTIONs Safety Goggles SKIN PROTECTIONT Rubber gloves, protective clothing. VENTILATIONT Local exhaust ventilation should be used to control airborn* dust. RESPIRATORY PROTECTIONs Toxic dust or supplied air rvspirator if dust Is not well controlled. ADDITIONAL INGREDIENTS ----------------------- EXPOSURE LIMIT$ EXPOSURE VALUE @*aT-. LIMITS TYPE AUTH Assonium Perfluoroalkyl Carboxylate 0.1 "/&3 TWA 3N SOURCE OF EXPOSURE LIMIT PATAT - ACOINT American Conference of Governmental Industrial Hygienists - 3hi 3M Exposure Guidelines ------- ------------------ - --- ----- 8. WEALTH HAZARD DATA ---------------- - -------- EYE CONTAC?s FC-143 can be Irritating to the eyes upon direct contact. Anteal studies Indicate FC-143 is moderately Irritating to the eyes. Airborne FC-143 "V cause eye injury consisting of corneal opacity. SKIN COWACTO FC-143 "y be absorbed through the skin. Slightly toxic when absorbed through the skin. FC-143 Is non-Irritating to the Skin. INHALATIONs FC-143 "y cause respiratory systes irritation from inhalation of dust. FC-143 can be considered moderately toxic by Inhalation on a single exposuret a median lethal concentration for a 4-hour exposure in the albino rat Is M_ailliqraas per cubic "ter. Repeated Inhalation exposure produced liver changes and elevated blood Organallmoride levels In rats. INOESTIONI FC-143 is considered mw*ratoly toxic frm a single oral aposure. The &cut@ oral L950 (rat) Is $40 ailllqr"s Per kilogram of :ody weight. FC-143 Is not torat"*nic (rats or rabbits). see ---------------------------------------- ------------ -- ------------------ Abbroviationss WD Not Determined WA - Not Applicable 156 3 RSDSO FC-143 FLUCRAD Irand Fluarachosical Surfactant 9&r-21-1999 PACE 4 ----------------------------------------------------------------------------- 8. HEALTH HAZARD DATA (coatinued) HWI 6329-100 ------------------------------------------------------------------------------ precautions for results @1 2 year study. -------------------------------------------------------------------------------- Abbroviationss WO Not Determined N/A - Not Applicablg ---------------------------------------------- ---- --- - ----- -------- -- Th* Information on this Data Sho*t represents our curro"t dgt& &ad b*st opinion as to the proper use and handling of this B&t*rt&l under normal conditions. Any use of the material which is not in Conformance with this 157 Data Shvet or which involves using the Baterial t" C006ination with aftr PP-ifteftsibilitvof the user. HWI 6329-100 APPENDIX B Individual Antemortem Observations IndividualBody Weights (g) Individual Food Consumption (g/wk) NOTE: Only observations other than normal are indicated in the Individual Antemortem Observations tables. 158 ANIMAL NUMBER 67451 67452 674c-2 67452 67453 67454 67454 674SS 67456 67456 674S7 674SS 67459 674S9 67460 67461 67461 67462 67463 67463 67464 67464 6746S 6746S 6746S 67466 67467 67468 67469 Hut 6381-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP I - 0 PPM T-SIGO - MALES UEEK 6 1 3 22 21 2 14 a 20 21 s 8 9 14 14 3 5 14 3 a 4 6 3 3 a 8 6 s 8 OBSERVATION ----------- INTERIM SACRIFICE 01/25/91 MALOCCLUSION ALOPECIA RECOVERY SACRIFICE OS/17/91 RECOVERY SACRIFICE 05/16/91 ALOPECIA TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 02/14/91 CHROMODACRYORRHEA RECOVERY SACRIFICE 05/16/91 INTERIM SACRIFICE 01/24/91 INTERIM SACRIFICE 02/14/91 SMALL MOVABLE TISSUE MASS TERIIINAL SACRIFICE 03/26/91 TERMINAL SACRIFICE 03/25/91 ALOPECIA INTERIM SACRIFICE 01/23/91 TERMINAL SACRIFICE 03/25/91 ALOPECIA INTERIM SACRIFICE 02/12/9) ALOPECIA INTERIM SACRIFICE 01/25/91 ALOPECIA BLOODY CRUST INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 01/2S/91 INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 02./13/91 LOCATION -------- RIGHT EYELID HIND RIGHT VENTRAL NECK 159 HWI 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP I - 0 PPM T-SISO - MALES ANIMAL NUMBER WEEK OBSERVATION ----------- LOCATION -------- 67470 67471 67472 67473 67474 67475 67476 67476 67477 67478 674?S 67479 67479 67479 67420 67481 67482 67482 674Z3 67483 67484 6748S 67486 674E,7 674Ek,l 6741,1'9 67490 14 22 a 14 a 14 4 6 6 4 a 3 A 22 8 a 6 14 16 21 a 14 5 14 14 s @-I TERMINAL SACRIFICE 03/26/91 RECOVERY SACRIFICE OS/17/91 INTERIM SACRIFICE 02/13/91 TERMINAL SACRIFICE 03/ZS/91 INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/22/91 MALOCCLUSION INTERIM SACRIFICE 01/2s/91 INTERIM SACRIFICE 01/2S/91 ALOPECIA INTERIM SACRIFICE 02/14/91 ALOPECIA MALOCCLUSION RECOVERY SACRIFICE 05/17/91 INTERIM SACRIFICE 02/12/91 INTERIM SACRIFICE 02/12/91 MALOCCLUSION TERMINAL SACRIFICE 03/22/91 ALOPECIA RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 01/23/91 TERMINAL SACRIFICE 03/2S/91 TERMINAL SACRIFICE 03/26/91 INTER!M SACRIFICE 01/24/91 RECOVERY SACRIFICE OS/16/91 160 ANIMAL NUMBER 67491 67492 67493 67493 67493 67493 67494 6749S 67496 67496 67497 67498 67499 67499 67499 67SOO 67SOI 67502 67SO3 67SO4 67SOS HWI 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP I - 0 PPM T-SISO - "ALES WEEI< 5 s 2 3 9 22 a a 20 21 22 a a 3 14 s 14 6 5 14 14 OBSERVATION ----------- INTERIM SACRIFICE INTERIM SACRIFICE 01/23/91 01/24/91 SORES ALOPECIA BLOODY CRUST RECOVERY SACRIFICE OS/17/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 02/12/91 WART-LIKE LESION RECOVERY SACRIFICE OS/16/91 RECOVERY SACRIFICE OS/17/91 INTERIM SACRIFICE 02/23/91 BLOODY CRUST ALOPECIA TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 01/23/91 TERMINAL SACRIFICE 03/2S/91 INTERIM SACRIFICE 01/2S/91 INTERIM SACRIFICE 01/24/91 TERMINAL SACRIFICE TERMINAL SACRIFICE 03/22/91 03/22/91 LOCATION -------- NECK NECK HEAD NECK 161 ANIMAL NUMBER ------ 67506 67507 67508 67SO9 67510 67511 67SIl 67511 67SIZ 67SI3 67SI4 67SIS 67SI6 67Sl7 67517 67sts 67519 67SZO 67SE0 67521 67521 67521 67521 67SZI 67S21 67522 67S23 67S23 HUI 6329-100 INDIVIDUAL ANTEMORTEN OBSERVATIONS CROUP 2 - I PPM T-5180 - MALES WEEK ---- 6 14 21 s 5 1 I s 6 ea 5 s 21 20 22 14 14 4 22 1 1 2 2 3 14 a 8 14 OBSERVATION ----------- INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/22/91 RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 01/24/91 INTERIM SACRIFICE 01/Z4/91 ALOPECIA BLOODY CRUST INTERIM SACRIFICE 01/24/91 INTERIM SACRIFICE 02/13/91 RECOVERY SACRIFICE OS/17/91 INTERIM SACRIFICE 01/24/91 INTERIM SACRIFICE 01/24/91 RECOVERY SACRIFICE OS/16/91 MALOCCLUSION RECOVERY SACRIFICE OS/17/91 TERMINAL SACRIFICE 03/26/91 TERMINAL SACRIFICE 03/26/91 MALOCCLUSION RECOVERY SACRIFICE OS/17/91 ALOPECIA BLOODY CRUST SORES BLOODY CRUST SORES TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 02/12/91 SOFT FECES TERMINAL SACRIFICE 03/26/91 LOCATION -------- NECK NECK BACK BACK NECK 162 ANIMAL NUMBER ------ 67s24 67SZ4 6752S 67526 67527 67528 67SP.9 67530 67530 67530 67530 67531 67S32 67S32 67532 67S33 67534 67S34 67535 67S36 67537 67S38 67S3$ 67S38 67S3$ 67538 67539 67S40 67S41 HUI 6399-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP 2 - I PPm T-5160 - MALES WEEK ---- 4 5 s 14 a 22 s 1 1 3 5 a 3 3 8 14 4 al 14 14 5 s 5 5 7 at a IB 14 OBSERVATION ----------- ALOPECIA INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 01/23/91 TERMINAL SACRIFICE 03/2S/91 INTERIM SACRIFICE 02/13/91 RECOVERY SACRIFICE 05/17/91 INTERIM SACRIFICE 01/24/91 ALOPECIA BLOODY CRUST SORES INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 02/12/91 ALOPECIA BLOODY CRUST INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/2S/91 ALOPECIA RECOVERY SACRIFICE OS/16/91 TERMINAL SACRIFICE 03/22/91 TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 01/24/91 ALOPECIA BLOODY CRUST BLOODY CRUST BLOODY CRUST RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/22/91 LOCATION -------- HEAD HEAD HEAD ABDOMEN LEFT FORELEG RICHT FORELEG 163 ANIMAL NUMBER ------ 67S42 67S43 67S43 67S43 67S44 6754S 67546 67547 67S48 67S49 67S49 67S49 67SSO 67551 67551 67552 67SS3 67SS3 67554 67SSS 67556 67557 67558 67SS9 67SS9 67SS9 67560 HUI 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP 2 - I PPM T-5180 - HALES WEEK ---- a 6 is 21 6 a a a ea 2 3 14 6 13 t4 14 2 14 a s 14 8 s 3 3 6 a OBSERVATION ----------- INTERIM SACRIFIICE Oe/13/91 MALOCCLUSION MISSING RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 01/2S/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/14/91 RECOVERY SACRIFICE OS/17/91 BLOODY CRUST ALOPECIA TERMINAL SACRIFICE 03/25/91 INTERIM SACRIFICE 01/ZS/91 SOFT FECES TERMINAL SACRIFICE 03/25/91 TERMINAL SACRIFICE 03/26/91 MALOCCLUSION TERMINAL SACRIFICE 03/25/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 01/23/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 01/23/91 ALOPECIA BLOODY CRUST INTERIM SACRIFICE 01/2S/91 INTERIM SACRIFICE 02/12/91 LOCATION -------TEETH BACK NECK 164 ANIMAL NUMBER 67S61 67561 67s6l 67S61 67S62 67S62 67S63 67563 67563' 67S64 67S64 6756S 67S66 67567 67S67 67S67 67S67 67S6$ 67S69 67570 67S70 67571 67S72 67S72 67S73 67S73 67574 67S7S 67S76 67577 67577 Hut 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP 3 - 10 PPM 7-5180 - MALES WEEK 2 2 2 22 4 6 2 2 14 3 14 6 5 16 16 Is 21 s 5 6 a 14 20 22 Is 2!:, 6 21 5 3 s OBSERVATION ----------- ALOPECIA SORES BLOODY CRUST RECOVERY SACRIFICE OS/17/9i ALOPECIA INTERIM SACRIFICE 01/2S/91 SORES BLOODY CRUST TERMINAL SACRIFICE 03/22/91 MALOCCLUSION TERMINAL SACRIFICE 03/25/91 INTERIM SACRIFICE 01/ZS/91 INTERIM SACRIFICE 01/24/91 MALOCCLUS-ION EPISTAXIS MISSING RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 01/24/91 INTERIM SACRIFITE 01/24/91 MALOCCLUSION INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/22/91 SWOLLEN RECOVERY SACRIFICE OS/17/91 MALOCCLUSION RECOVERY SACRIFICE 05/17/91 INTERIM SACRIFICE Oi/2S/9i RECOVERY SACRIFICE 05/16/91 INTERIM SACRIFICE 01/23/91 MALOCCLUSION INTERIM SACRIFICE 01/23/91 LOCATION -------HEAD HEAD NECK NECK TEETH TAIL 165 ANIMAL NUMBER 67S78 67S79 67580 67581 67582 67583 67584 67SSS 67SS6 67SS7 67SSS 67SSS 67SS9 67590 67s9o 67591 67S91 67S91 67S91 67591 67591 67S92 67593 67594 67S9S 67s9s 67596 67S97 NWI 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP 3 - 10 PPM T-SISO - MALES WEEK S. 21 a a a a a a a 8 a 22 21 4 s 3 3 3 3 4 5 5 21 a 3 5 14 s OBSERVATION ----------- INTERIM SACRIFICE 02/12/91 RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/12/91 INTERIM SACRIFICE 02/12/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/12/91 MALOCCLUSION RECOVERY SACRIFICE 05/17/91 RECOVERY SACRIFICE OS/16/91 ALOPECIA INTERIM SACRIFICE 01/24/91 SORES SORES BLOODY CRUST BLOODY CRUST ALOPECIA INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 01/24/91 RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 02/13/91 ALOPECIA INTERIM SACRIFICE 01/23/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 01/24/91 LOCATION -------- BACK HEAD BACK HEAD 166 ANIMAL NUMBER 67S98 67599 67S99 67600 67601 67602 67602 67603 67604 67605 67606 67607 67608 67609 67610 67611 67612 67613 67613 67614 67615 Hut 6329-100 INDIVIDUAL ANTE"ORTEM OBSERVATIONS CROUP 3 - 10 PPM T-SISO - MALES UEEK 14 3 5 14 6 2 a 14 14 14 a 14 14 22 14 14 a a 14 a 14 OBSERVATION ----------- TERMINAL SACRIFICE 03/26/91 MALOCCLUSION INTERIM SACRIFICE 01/23/91 TERMINAL SACRIFICE 03/26/Si INTERIM SACRIFICE 01/25/91 MALOCCLUSION INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/22/91 TERMINAL SACRIFICE 03/22/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/26/91 TERMINAL SACRIFICE 03/25/91 RECOVERY SACRIFICE 05/17/91 TERMINAL SACRIFICE 03/2S/91 TERMINAL SACRIFICE 03/25/91 INTERIM SACRIFICE 02/12/91 BLOODY CRUST TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/ZS/91 LOCATION -------- NECK 167 ANIMAL NUMBER ------ 67616 67617 67618 67619 67620 67621 67621 67621 67622 67622 67622 67623 67624 6762S 67626 67627 67628 67629 67630 67631 67632 67633 67634 67634 67634 67635 67636 NWI 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP 4 - 30 PPM T-5180 - MALES WEEK ---- 14 a 14 s 6 a 2 14 9 9 22 14 14 6 21 5 21 8 6 s 5 6 2 4 a 14 6 OBSERVATION ----------- TERMINAL SACRIFICE 03/2S/91 INTERIM SACRIFICE 02/13/91 TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 01/24/91 INTERIM SACRIFICE 01/2S/91 ALOPECIA BLOODY CRUST TERMINAL SACRIFICE 03/26/91 MALOCCLUSION EPISTAXIS RECOVERY SACRIFICE OS/17/91 TERMINAL TERMINAL SACRIFICE SACRIFICE 03/25/91 03/26/91 INTERIM SACRIFICE 01/25/91 RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 01/23/91 RECOVERY SACRIFICE OS/16/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 01/2S/91 INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 01/ZS/91 ALOPECIA BLOODY CRUST INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 01/2S/91 LOCATION -------HEAD RIGHT FOREPAW 168 ANIMAL NUMBER 67637 67638 67639 67640 67641 67641 67642 67643 67643 67644 6764S 67646 67647 67648 67649 67649 67649 67649 67649 67650 67651 676SI 67651 67652 676S3 676S3 676S3 67654 67fiss INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP 4 - 30 PPM T-5180 - MALES HUI 6329-100 WEEK 21. a a 5 2 14 a 12 14 8 14 8 a s 2 a 4 7 22 14 5 16 22 14 12 12 14 s s OBSERVATION ----------- RECOVERY SACRIFICE OS/16/gi INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 01/23/91 ALOPECIA TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 02/12/91 SMALL MOVABLE TISSUE MASS TERMINAL SACRIFICE 03/25/91 INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 01/24/91 SORES BLOODY CRUST ALOPECIA MALOCCLUSION RECOVERY SACRIFICE 05/17/91 TERMINAL SACRIFICE 03/22/91 ALOPECIA MALOCCLUSION RECOVERY SACRIFICE OS/17/91 TERMINAL SACRIFICE 03/ZS/91 WART-LIKE WART-LIKE TERMINAL LESION LESION SACRIFICE 03/2S/91 INTERIM SACRIFICE 01/24/91 INTERIM SACRIFICE 02/14/91 LOCATION -------- HIND LEFT VENTRAL NECK NECK HIND LEFT VENTRAL LEFT HIND PAW 169 ANIMAL NUMBER ------ 676r-%6 67656 676S7 676S7 676S8 676S9 67660 67661 67662 67663 67663 67663 67664 67664 67664 67665 67666 67666 67667 67667 67668 67668 67668 67669 67670 67670 67670 NWI 6329-104 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP 4 - 30 PPM T-5180 - MALES WEEK ---- s a 4 8 14 5 22 a a 4 4 5 7 is 21 s 2 22 5 8 s s 14 14 s 19 21 OBSERVATION ----------- SWOLLEN INTERIM SACRIFICE 02/14/91 AI:DPECIA INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 41/24/91 RECOVERY SACRIFICE 05/17/91 INTERIM SACRIFICE INTERIM SACRIFICE 02/12/91 02/13/91 SQUINTING CHROMODACRYORRHEA INTERIM SACRIFICE 01/23/91 ALOPECIA SMALL MOVABLE TISSUE MASS RECOVERY SACRIFICE 05/16/91 INTERIM SACRIFICE 01/23/91 MALOCCLUSION RECOVERY SACRIFICE OS/17/9i ALOPECIA INTERIM SACRIFICE 02/13/91 MALOCCLUSION RHINORRHEA TERMINAL SACRIFICE TERMINAL SACRIFICE 03/22/91 03/22/91 ALOPECIA SMALL MOVABLE TISSUE MASS RECOVERY SACRIFICE 05/16/91 LOCATION -------LEFT MIND PAW RIGHT EYE RIGHT EYE HIND RIGHT VENTRAL 14IND RIGHT VENTRAL 170 ANIMAL NUMBER NWI 6329-100 INDIVIDUAL ANTEMORTEN OBSERVATIONS GROUP 5 - 100 PPM T-5180 - MALES WEEK OBSERVATION ----------- LOCATION -------- 67671 3 67671 14 67672 11 67672 14 67673 a 67673 a 67673 4 67673 14 67674 21 6767S 4 6767S s 67676 12 67676 13 67676 is 67676 is 67676 is 67676 16 67676 Is 67676 20 67676 20 67676 20 67676 ea 67677 14 67678 21 67679 14 67680 a 676SI ;2 67681 6 67682 1 67682 1 67682 s 67(>83 ai MALOCCLUSION TERMINAL SACRIFICE 03/25/91 MALOCCLUSION TERMINAL SACRIFICE 03/2S/91 ALOPECIA BLOODY CRUST BLOODY CRUST TERMINAL SACRIFI.CE 03/26/9t RECOVERY SACRIFICE 05/16/91 ALOPECIA INTERIM SACRIFICE 01/24/91 SWOLLEN BLOODY CRUST SWOLLEN STIFF DOES NOT USE SWOLLEN MISSING THIN MUNCHED POSTURE NECROTIC RECOVERY SACRIFICE OS/17/91 TERMINAL SACRIFICE 03/26/91 RECOVERY SACRIFICE 05/16/91 TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 02/12/91 ALOPECIA INTERIM SACRIFICE 01/25/91 ALOPECIA BLOODY CRUST INTERIM SACRIFICE 01/23/91 RECOVERY SACRIFICE OS/16/91 HEAD RIGHT FOREPAW BOTH MIND PAWS TAIL RIGHT FOREPAW TAIL BOTH HIND LEGS BOTH FOREPAWS TIP OF TAIL TAIL NECK 171 ANIMAL NUMBER ------ 67684 67684 67618S 6768S 6768S 676P.5 67686 67687 67688 67688 67688 67688 67688 6768e 67689 67699 67690 67691 67691 67692 67693 67694 67694 67694 6769S 67696 67697 67698 67699 67700 HUI 6329-100 INDIVIDUAL ANTEHORTEM OBSERVATIONS GROUP S - 100 PPH T-SISO - MALES WEEK ---- 9 21 2 2 2 4 14 6 a 10 10 10 11 14 3 .5 5 10 14 5 5 2 2 21 14 5 14 6 a 14 OBSERVATION ----------- LOCATION -------- MALOCCLUSION RECOVERY SACRIFICE OS/17/91 ALOPECIA SORES BLOODY CRUST MORIBUND SACRIFICE 01/16/91 TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 01/2S/91 ALOPECIA THIN MALOCCLUSION FEW OR NO FECES SOFT FECES TERMINAL SACRIFICE 03/e6/91 CROOKED INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 01/23/91 MALOCCLUSION TERMINAL SACRIF.ICE 03/ZS/91 INTERIM SACRIFICE 01/24/91 INTERIM SACRIFICE 01/24/91 ALOPECIA BLOODY CRUST RECOVERY SACRIFICE OS/16/91 TERMINAL SACRIFICE 03/e2/91 INTERIM SACRIFICE 01/24/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 01/2S/91 INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/25/91 NECK NECK TIP OF TAIL LEFT FORELEG 172 ANIMAL NUMBER ------ 67701 67702 671'03 67704 67705 67706 67706 67707 67707 67708 6770P, 67708 67709 67710 67711 67712 67713 67713 67714 6771S 67716 67716 67717 67717 67718 67719 67719 67-1,20 NWI 4329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP S - 100 PPM T-5180 - MALES WEEK ---- 21 8 8 22 a 4 22 a a 1 I a 8 a 8 14 4 6 14 s 7 14 2 s a 3 a 14 OBSERVATION ----------- LOCATION -------- RECOVERY SACRIF.ICE OS/i6/9i INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/14/91 RECOVERY SACRIFICE 05/17/91 INTERIM SACRIFICE 02/13/91 ALOPECIA' RECOVERY SACRIFICE 05/17/91 ALOPECIA INTERIM SACRIFICE 02/12/91 ALOPECIA BLOODY CRUST NECK INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE OP-/12/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/26/91 ALOPECIA INTERIM SACRIFICE 01/25/91 TERMINAL SACRIFICE 03/2S/91 INTERIM SACRIFICE 01/24/91 ALOPECIA TERMINAL SACRIFICE 03/22/91 ALOPECIA INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 02/14/91 MALOCCLUSION INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/22/91 173 ANIMAL NUMBER ------ 67721 67722 67722 67723 67724 6772S Hut 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS GROUP S - 100 PPM T-5180 - MALES WEEK ---- 8 s a 22 a s OBSERVATION ----------- INTERIM SACRIFICE 02/13/91 MALOCCLUSION INTERIM SACRIFICE 02/12/91 RECOVERY SACRIFICE 05/17/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 01/23/91 LOCATION -------- 174 ANIMAL NUMBER ------ 67726 67727 67728 67729 67730 67731 67732 67733 67734 67735 67736 67737 67738 67739 67740 67741 67742 67743 67744 67745 67746 67746 67747 67748 INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 6 - 0 PPM T-5180 - MALES NWI 6329-100 WEEK ---- s 5 a 8 14 a 14 5 a 5 14 P, 14 a 14 s 5 a 14 5 8 14 14 14 OBSERVATION ----------- INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 61/2S/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/25/91 INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 02/12/91 INTERIM SACRIFICE 01/24/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/eS/91 INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 01/23/91 INTERIM SACRIFICE 01/24/91 INTERIM SACRIFICE 02/13/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 01/24/91 SWOLLEN TERMINAL SACRIFICE 03/eS/91 TERMINAL SACRIFICE 03/26/91 TERMINAL SACRIFICE 03/22/91 LOCATION -------- TAIL 175 ANIMAL NUMBER ------ 67749 677SO 67751 677SZ 677S3 677S3 677S4 677SS 677S6 677S7 6775e 677S9 67760 67761 67762 67763 67763 67764 67765 6776S 67766 67766 67767 67768 67769 67-170 HWI 6329-140 INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 6 - 0 PPM T-5180 - MALES WEEK ---- 14 a s a 0 14 14 14 5 a s 5 2 P, s 2 a 7 a 14 a s OBSERVATION ----------- TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE 02/13/91 INTERIM SACRIFICE 01/ZS/91 INTERIM SACRIFICE 02/14191 CHROMODACRYORRHEA INTERIM SACRIFICE 02/14/91 TERMINAL SACRIFICE 03/25/91 TERMINAL SACRIFICE 03/22/91 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 01/2S/91 INTERIM SACRIFICE 02/14/91 INTERIM SACRIFICE 01/24/91 TERMINAL SACRIFICE 03/22/91 INTERIM SACRIFICE Ol/eS/gl INTERIM SACRIFICE ALOPECIA INTERIM SACRIFICE 01/23/91 02/13/91 INTERIM SACRIFICE MALOCCLUSION INTERIM SACRIFICE 01/2S/91 02/13/91 MALOCCLUSION INTERIM SACRIFICE 02/12/91 TERMINAL SACRIFICE 03/eS/91 INTERIM SACRIFICE 02/12/91 INTERIM SACRIFICE 01/25/91 INTERIM SACRIFICE 01/2S/91 LOCATION -------- RIGHT EYE 176 INDIVIDUAL BODY WEIGHTS 101 13 WX DIETARY TOXICITY STUDY WITH T-5180 NUMBER 0 1 2 3 4 5 6 7 1 OROUP I MALES - CONTROL to 11 67451 209.1 285.0 320,4 301.1 412.3 444.5 - 07452 67453 187.1 217.1 236.1 210.1 294.4 330,7 333.3 380.8 3 9.1 398. :23.0 4S$.S 415.0 435.0 452.5 477.1 410.5 494.5 4 674S4 67455 206.2 200.4 26?.3 2S9.0 326.2 311.9 367.7 10.3 438.1 484.1 463.0 506 1 527.1 479.7 496.S 544.8 SIS.6 562.5 531 .8 577.7 540.1 S 5 61456 67457 117.5 243.1 284.7 350.3 32S.1 302.4 300.6 418. 388.1 430.3 407.7 452.8 421.4 A 4ss.? 0?458 202.7 201.4 254.7 251.9 297.1 304.5 328.4 340.3 356.4 375.7 -% 390.1 465'5 475*6 418"0 4 i i 81459 67460 186.5 210.0 243.1 266.0 294.7 331.3 373.9 401.2 411.5 421.3 429.6 436.5 454.2 a 463.1 6 461,8 a 411.6 67461 204.1 260.1 317.9 306.8 354.8 343.3 391.1 389.9 422.5 A 437.0 a 455.6 A 469.2 a 41S.3 501.6 a 507,9 4 5 67462 67463 212.2 205.1 269.5 270.0 324.1 31S.9 361.2 354.2 39S.9 424.9 4:7.0 473.5 494.2 520.A 7 540.1 a 555.6 5 67464 109.3 261.7 323.6 361.9 3 3.8 427.1 391.1 426.7 4 7.5 464.7 67465 67466 208.2 210.3 267.0 264.7 307.5 321.6 331.6 351.9 360:.10 387.0 4. 40240 414*3 67467 214.1 270.2 315.3 354.1 393.2 434.4 423.7 459:3 415.*6 67468 201.2 262.3 302.6 339.1 373.1 ob 67469 67470 208.3 270.6 324.1 363.3 391.9 431.S 453.3 47 .7 67471 196.9 210.3 255.3 270.8 312. 318.5 341.8 354.3 379.1 394.0 402.0 425.3 37.1 455.1 472 7 415 1 490.7 4 67412 67473 201.3 267.3 334.0 379.0 424.1 455.9 43.2 44 2 55 :61.3 504.3 492.1 517'.3 528'2 539.0 S 208.3 273.5 67474 205.5 203.1 337,6 311.: 371.5 355.S 423.2 392.4 446.5 424.0 "74. 1 442.2 492.2 451.2 S17.a1 a 542.A9 A 554 *9 Sao I s 07475 67476 201.3 213.3 272.3 2651.7 324. 321.0 367.0 361.2 407.2 420.8 449.9 471.S 487.7 510.1 523.7 i 541.2 S 6?4?7 104.0 2 0.2 305.3 349.0 394.2 390.7 425.S 425.4 6?471 67479 204.0 196.0 2tO.4 2 1.8 311.0 302.3 353.0 346.7 389.8 367.9 41S.? 439.1 451.0 67410 07411 208.9 197.3 266.4 255.1 315.3 29S.4 349.1 329.6 391.5 363.S 55.3 392.S 418.3 392.3 316.0 01.5 434.3 1908.4 430.6 453 0 423.-7 430 6 449.1 4SS.? 441.1 44 67412 67483 67414 189.7 216.6 241.4 277.3 284.6 323.0 324.S 354.9 3 382.8 413.7 .5 411.8 9 442 8 451 6 473.S 490.2 49 208.2 264.6 431 8 452.1 472.2 495.6 510.0 6?485 206.7 261.5 315.1 321.7 360@O 363.3 402.9 405.9 434.S 434.6 4552'4 470 473.6 479.4 520.8 S3 67486 200.5 261.3 30S.0 339.7 379.6 & Soo.$ 529.4 548.0 SOS.3 57 -67417 67488 193.1 223.0 252.4 291.9 299.1 34S.1 331.1 360.0 398.2 413 421 4 4SO 3 467.5 481.7 496 4 SO 67489 207.9 261,4 296.6 392.0 329.1 435.5 355.0 469.7 a 404. a 520'4 535'.0 sss 7 a 567 0 Sao's so 67490 67491 203.1 201.7 2!8.1 2 S.6 317.2 296.9 3S7.9 331.3 394.6 421.1 431 9 458'6 410 0 494.1 i 508.9 It S13.1 52 67492 226.8 290.9 341.2 385.4 365.9 424.6 i 67493 189.8 245.4 293.3 323.4 349.3 361.9 384.3 407.6 425.2 437.5 448.6 463.4 47 - DEAD ANIMAL AlffM NUMBER 14 Is 1$ OROUP 67451 674S2 67453 67454 674S5 674se 674S? 674si 674so 7460 :?461 07462 07463 67404 6746S 67460 67487 67468 67469 67470 67471 67472 67473 67474 6747S 67476 67477 07471 674?9 67410 67481 67482 67483 67414 67485 67486 67487 67481 67489 67490 67491 67492 67493 I MALES - CONTROL a S12.2 619.3 a S27.2 623.S a 533.6 631.6 Sll.g 525.8 540.4 S?1.3 a A a a a a a 490.6 581.4 605.5 509.0 514.2 556.4 564 8 580.1 545.7 404.3 SS3.5 497.2 566.5 494.4 - DEAD ANIMAL 17 0 S40.6 636.8 541.5 015.1 526 6 589.4 583.5 505.7 INDIVIDUAL BODY WEIGHTS (0 13 WK DIETARY TOXICITY STUDY WITH T-5180 WEEKS is 19 20 21 S41.4 641.2 543.8 554.7 $49.S SSI.2 Ss5.0 659.7 561.7 579.0 616.1 628:8 638.1 648.0 528 1 524 0 i 517 5 i 524 7 585.7 597.5 001.1 574.3 S07.9 sl?.l 596.0 509.9 SOI.3 511.0 00 ANIMAL NUMBER GROUP 67494 67495 67496 67497 87498 67499 67500 67501 67502 67SD3 67504 67505 0 2 I MALES - CONTROL 206 5 203.6 204.S 187.8 208.5 212.3 216,0 202.6 181.6 197.2 205.9 204.2 258.9 271.6 256.3 242.0 261.3 274.5 269.3 256@O 2SO.3 259@s 267.2 257.1 305.4 321.2 209.6 294.5 321.6 331.6 317.1 302.5 302.9 309.1 324.6 304.3 3 333.0 370.4 330.1 341.0 366.9 377,0 354.4 331.1 344.4 352.4 362.9 342.6 INDIVIDUAL BODY WEIGHTS IQ 13 WK DIETARY TOXICITY STUDY WITH T-5110 WEEK-S-- 4 -- 5 6 7 9 367.2 400.1 361.6 373.9 410.0 417.3 314.7 373.7 379.4 38?.S 403.4 37S.2 389.7 433.9 386.8 403.1 431.1 4S2.4 0 401.2 412.S A 437.1 402.8 414,4 454.0 404.1 421.1 461.3 479.0 % 41S.4 467.0 427.8 430.0 474.1 421.6 440.0 481.9 502.6 436.7 492.5 44S.2 435.4 4SO.2 512.8 455.A1 509.5 463.3 44S.2 478.2 536 65 i 475.5 522.6 416.7 10 11 4S2 7 413,5 SSI 8 473.9 463.8 501.2 560.4 a 492.4 SSO.5 494.1 573.0 S13.2 DEAD ANIMAL XHTW NUMBER GROUP 87494 67495 67496 67497 67428 67499 G?SOO 07501 07502 67503 07504 6750S INDIVIDUAL BODY WEIGHTS to 13 WK DIETARY TOXICITY STUDY WITH T-SIIO 14 15 is 17 to to wttK250 21 I MALES - CONTROL 481.9 521.3 480 8 533 S 411 1 S40.9 491 1 SS9.7 492.8 56S.3 49S.S 576.2 499.2 590.2 S92.s DEAD ANIMAL 00 C3 INDIVIDUAL BODY WEIGHTS (0 13 WK DIETARY TOXICITY STUDY WITH T-5110 NUMBER 0 1 GROUP 2 14ALES - 67506 olsoy $ISO$ 67509 G?SIO 67511 67512 67513 67514 0?515 6?516 67517 elsis 67519 67520 67S21 61522 67523 67524 67525 6?526 67527 67S2$ 67529 67530 67531 6?532 67533 6?534 57535 67536 6?537 07531 SYS39 67540 67541 61542 67543 67s44 6IS45 67546 67547 SIS43 202.0 204.4 199.8 223.6 201.3 221.4 200.3 207.9 220.2 192.0 201.3 212.5 201.8 201,2 216.4 202.4 199.3 223.1 ISS.2 214.8 196.8 195.9 197.6 201.8 223.5 213.1 213.2 201.2 211.0 197.8 201.6 180.7 192,3 201-S 217.1 207.1 194.3 200.0 198.6 200.9 212.6 210.6 190.8 256.0 259.1 259.4 287.3 2S6.4 271.S 248.9 264.3 282.1 240.4 260.5 275.3 254.0 2S4.3 270.9 259.1 263.8 216.3 235.3 277,2 254.3 257.5 255.2 250.5 279.8 277.5 271.2 261.0 210.1 2S2.0 264.6 232.0 256.2 2S$.3 283.2 278.1 245.4 261.2 254.3 2S9.5 268.7 271.9 243.8 DEAD ANIMAL 2 3 1.0000 PPM 299.4 309.3 308.2 342.4 303.0 316.2 292.0 305.0 334.1 287.8 311.8 324.4 303.7 301.1 317.2 307.2 311.8 342.2 283.5 330.4 302.3 312.7 298.8 300.5 330.0 329.9 320.2 310.1 330.S 203.3 315.1 275.7 313.8 317.8 343.3 323.7 285.0 317.1 298.2 301.4 311.3 313.7 211.1 331.8 343.6 346.5 390.3 342.0 343.6 32S.? 342.5 370.1 328.1 357.7 363.0 333.4 33S.4 355.2 339.0 373.0 380.9 321.3 370.3 334.8 353.9 338.7 341.7 372.2 374.2 361.2 3SO.8 374.8 341.9 340.2 301.0 357.9 361.2 393.6 363.3 314.6 35?.g 334.2 333.7 347.9 3S$.9 321.7 4 5 362.0 38$.g 388.2 432.1 377.5 374.0 361.0 370.8 402.1 365.9 392.0 390.6 3$S.0 372.7 383.0 373.1 412.3 419.7 357.0 409.9 366.9 389.7 365.0 380.9 401.1 418.4 399.2 3$S.7 408.6 375.9 385.2 337.9 397.1 391.6 439.3 393.8 351.1 397.7 361.2 360.2 384.1 383.1 353.2 394.8 400.5 406.9 390.0 400.7 421.4 428.7 342.5 38?.2 406.6 402.2 452.0 447.9 389.4 427:9 395.S 450.2 431.1 411.8 439.5 403.S 419.0 ft 422.9 431.8 477.1 421.7 377.2 432.2 389.4 380.7 418.2 405.5 386.3 6 413.5 429.5 421.2 411.0 425.4 4SI.8 445.? 394.7 414.2 421.7 423.6 424.7 46$.S 404.7 453.1 417.0 476.5 451.5 447.3 482.S 424.2 442.8 449 A 5 459:5 504.7 442.7 397.9 440.0 40OA0 442 5 432.'1 399.4 7 421.0 453.8 441.4 432.5 449.8 413.1 461.6 407.5 432.0 438.6 446.7 513.0 495.9 415.9 477.6 437.1 503.7 473.7 460.8 442.3 440.1 462.4 471,a4 416.9 532.4 4S$.2 417.0 4S?.? 421 2 458.0 439.0 417.4 8 461.3 461.3 474.4 505.7 491.6 41S.1 454.1 458.? 473.0 1% 513.2 433.a 9 449.2 415.0 504.9 460.3 469.3 A 491.5 412.7 a 479.9 433.4 9 481 5 416 9 413 7 529 6 soo.4 425.7 467.7 474.0 492.1 540 2 453.5 467.0 503,3 510.3 493.0 SOS.2 a 507.1 499.9 Soo a 1 4S2.7 10 11 494 0 504 6 5 491 8 502 5 s 513 1 525 6 S 543.2 564.3 5 sts.6 53S.1 5 432.2 448.7 4 474.0 417.6 4 485.5 496.5 4 514.9 524.2 5 SS3 9 575.8 5 46S.9 483.4 49 477.8 489 a 3 49 524.0 539.3 55 531.7 546,9 SS 491.4 516.8 S3 518.5 538.0 54 A S27.1 541.a1 55 522.5 S33.0 S4 511.2 514.02 Sl 46S.0 471.8 47 ANTWE NUMBER GROUP 14 is 2 MALES - 87506 6750? 67508 67509 67510 $7511 67S12 07513 87514 O?SIS 67516 67517 67518 67519 07520 67521 67522 SYS23 67524 SYS25 67526 61527 67528 07529 67530 67531 67532 6?533 6?S34 6753S 67536 67537 67538 GIS39 67540 67541 67542 67543 67544 67545 SIS46 $7547 B?S4$ 522.9 570.6 601.8 569.8 405.0 516.3 573.6 004.4 S12.2 492.5 DEAD ANIMAL 528.0 S84.1 017.9 577.3 S24.8 515.9 513.9 614.9 517.2 491,7 Is- 17 1.0000 PPM S34 4 538 2 600.2 $25.6 593.1 530.9 621.0 631.1 611.7 530.9 529.3 533.1 592.6 601.3 640.8 641.2 516.0 514.6 SOI.4 523.3 INDIVIDUAL BODY WEIGHTS 10 13 WK DIETARY TOXICITY STUDY WITH T-5180 to 19 20 21 537 3 549 3 SSO.2 622.6 645.0 612.0 531.1 637.9 OSS.4 621.2 S34.2 OSI.S $43.4 600.6 545.1 673.8 017.4 543.1 537.5 545.3 550.3 564.2 004.5 606.6 611.6 649.1 682.7 077.0 528.8 535.0 518.3 516.0 537.1 S46.7 550.0 IRTW NUMBER GROUP 7549 :75so SYSSI 67SS2 GISS3 67SS4 a sss 8 S;! $75 67SSI S?SS09 0756 0 1 2 MALES - 214.7 191.2 209.0 221.9 203.4 218,7 207.4 200.3 211.5 221.5 22S.0 206.7 272.0 241.5 276.8 292.2 2S?.3 276.1 261.9 258.7 2#3.3 279.3 200.1 261.9 2 3 1.0000 PPM 323.4 284.1 337.S 347.2 299.7 322.6 31$.S 307.7 330.2 331.7 349.2 310.4 381.1 327.9 380.0 382.1 343.4 364.1 356.1 343.1 374.3 373.2 403.7 345.0 INDIVIDUAL BODY WEIGSHTS (01 13 WK DIETARY TOXICITY TUDY W T" T-5180 WEEKs 4 5 7 a 9 10 40S.1 3SO-8 423,1 418.1 381.8 402.6 391.7 376.4 413.2 409.3 447,6 389.0 443.S 311.1 AS3.S 452.7 411.7 432i.t5 407.3 44S.2 482.6 428.8 465.0 479.8 4?S.1 436.4 4S4.6 ik 429.7 462.1 439.8 491.9 S02 9 S02.1 455.2 477.1 445.9 482.9 4SO.2 S20.7 541.0 S54.7 52149 S34*S 54063 S2$ 4 553'.1 566'7 460..$ 497.6 517.2 46S.? 493.6 494.9 it S69.0 S5268 S#2.0 527.6 507.7 DEAD ANIMAL ANTM NUMBER OROUP 67549 67s5o 67ssi 67SS2 ?SS3 :7SS4 eisss 07556 OYSSY 7551 selsso0 6756 --14 15 2 04ALES 15 17 1.0000 PPM INDIVIDUAL BODY WE107TSY 13 WK DIETARY TOXICITY S UD (OWITH T-SIIO WEEKS is 19 20 21 DEAD ANIMAL INDIVIDUAT BODY WE12HTS 13 WK DIETARY TOXICITY TUDY IWGITH T-5180 AW7W NUMBER 0 1 GROUP 3 MALES - 67561 67562 61563 67564 $?SOS sisse SYS67 07561 SYS69 67570 67s?l 6TS72 67573 67S74 sis7s 67576 675?7 67571 67519 S?SIO 67581 67512 67583 6?584 675$S 67S$6 67587 6?511 67sto SYS90 6?s9l 6?s92 67593 6IS94 6759S 67596 47s97 67591 67S09 67600 67601 67602 67603 204.6 211.3 214.7 221.2 195.9 203.2 213.0 20S.3 184.6 211.7 181.1 212.8 20S.3 199.3 193.0 200.4 207.3 206.4 189.0 213.4 210.1 206.1 196.8 209.4 188.2 182.4 219.6 188.3 194.2 197.4 195.1 190.1 197.7 211.1 199.2 185.0 186.5 186.3 209.4 212.4 210.7 220.7 216.0 259.7 272.4 212.2 213.S 250.4 263.0 271.2 2S$.S 242.7 293.2 232.9 270.3 2SI.4 2SI.0 249.S 251.1 260.2 260.1 24S.4 262.S 268.9 262.9 247.8 266.3 241.S 230.6 276.8 242.2 255.0 249.8 248.8 235.7 255.1 277.5 2S4.3 234.3 242.0 239.1 26S.? 27$.S 278.0 216.2 203.0 - DEAD ANIMAL 2 3 10.0000 PPM 300.1 320.4 2S6.6 333.9 295.4 234.2 323.1 226.2 292.3 340.0 212.4 31S.4 304.5 299.4 308.5 299.0 296.9 312.2 292.3 30S.2 318.3 311.0 288.9 321.2 28S.8 273.S 324.0 289.5 311.S 300.0 294.2 270.8 314.0 334.8 301.4 219.2 212.6 281.8 312.4 330.8 337.0 337.7 2S4.7 34S.2 366.1 378.8 376.S 332.0 3S4.0 306.6 343.7 320.7 382.5 320.6 351.4 333.8 332.0 359.2 334.9 319.0 348.2 333.3 341.1 3SS.3 353.2 330.2 367.0 324.1 306.7 366.3 326.9 3SS.5 331.0 333.7 299.2 36S.S 379.3 355.8 317.0 319.2 32S.0 344.9 364.1 384.8 368.3 389.0 4 374.6 401.4 417.4 413.2 360.S 393.1 402.9 377.9 3SO.7 421.5 350.2 310.9 374.4 38S.7 404.2 360.5 34?.9 380.2 368.3 370.6 391.2 388.3 369.7 408.5 360.7 336.0 405.7 364.6 318.7 364.0 359.8 321.0 409.3 417.9 390.3 346.3 356.4 360.2 311.S 398.1 434.6 409.1 430.8 WEEKS 5 0 401.9 433.2 453.1 443.2 390.0 a 43S.0 424.0 474.9 466.1 $1 ok 481.5 448.1 370.1 408.1 400.8 394.8 440.4 451.7 224.3 433.2 421.1 474.S 411.5 402.7 393.7 420.,3 408.9 396.1 438.6 307.3 361.7 432.6 400.2 429.1 438.5 425.7 416.1 446.7 433.8 418.0 464.1 410.1 312.0 447.3 41S.6 4S7.4 444.6 451.0 dk 375.3 Is 390.5 429.6 477.0 439.6 471.9 474.6 477.4 It 393.1 a 414.1 0 4S4.3 0 466.5 492.4 7 a 442.0 499 9 487 1 460.9 524 4 506.3 488.0 S14.7 47I.S 413.0 444.4 428.6 a 496.2 431.5 467.7 447.7 It 527.8 456.1 442.9 427.4 464.1 446.1 437.9 485.9 42S.9 398.2 474.0 439.1 473.9 46S.6 447,3 499.3 496 2 SOO.8 413.6 438 a 6 480.0 485 4 3 S14.'0 519.4 429.9 4SS 9 a 49S.3 543.4 9 to 11 479.7 552 0 3 $25.7 S3?.4 494.1 SO$ 0 S41.5 554.4 SOS.7 S90 3 SS4,6 i S70.44 445.9 474,5 4S9.9 a 548.2 458.1 484.1 46$.S S64.3 470.6 495.4 472.6 S21.7 479.1 494.7 soo.6 4SS.4 SIO.4 478.6 523.8 491.8 S36.3 S53.3 S58.4 581.3 444.1 476.01 516 & 1 a S66.5 456.2 484.S S36.6 570 7 474.2 Agg 4 7 553.3 a S83 I ANIMAL NLN40ER GROUP 14 is 3 MALES - 67561 $7562 $7563 67564 elses 67s66 7567 6 07I5S669, 7570 6 7571 SIS?2 GIS?3 7574 :7575 67576 67577 67571 07579 7580 687581 67582 75883 6675 4 7585 667s8$76 67S 67588 67589 67590 67591 07592 IS93 6 7s94 67595 67596 67597 07598 7599 6 7600 67601 67602 67603 54?.S 562.7 616.5 631.4 525.2 505.6 626'3 527.0 S13.4 64741 536.S 542.5 521.2 563.2 ol 521.9 568.1 630.7 548mO a a DEAD ANIMAL is 17- 10.0000 PPM S74.5 520.0 614.1 622.1 531.9 521.1 659*2 537.5 S29.2 67345 551.6 570.6 538.3 582.6 546.2 592.9 664*3 67645 INDIVIDUAL BODY WEII"Ts 13 WK DIETARY TOXICITY TUDY (WOlt" T-5110 -WEEKS is to 20 21 587.1 597.2 $05.7 613.9 012.9 636.4 037.4 52S.5 490.3 67468 i 532.4 500.8 087*8 539.9 516.0 704*0 544.0 S25.2 561.6 S74.3 583.0 551.4 597.8 1% 503.3 603.0 569.0 612.4 581.5 686.6 694.3 704.0 C)D XNTW NUMBER GROUP 07604 $?SOS 67606 6760? 67601 67609 67610 67611 67612 67ol3 0?614 sisis INDIVIDUAL BODY WEIG"TS 13 WK DIETARY TOXICITY STTM)Y IWGITH T-SIIO 0 1 3 MALES - 221.4 208.3 201.1 19?.3 194.? 21S.3 212.0 193.S 190.9 210.1 20S.6 219.0 273.8 264.9 263.2 2SI.6 2S2.3 216.2 260.1 246.0 246.S 269.S 270.2 273.3 2 3 10.0000 PPM 320.0 310.4 316.S 209.0 294.2 338.2 313.2 287.2 291.0 323.7 323.9 317.0 362.2 3S2.7 350.1 324.7 327.3 384.9 356.2 32S.4 330.4 364.7 362.2 35S.1 4 390.6 389.3 3$S.7 3SS.2 360.1 424.7 333.7 360.0 375.2 403.4 39S.9 379.8 421.1 41S.5 417.8 381.4 3#S.0 4S$.l 411.4 3$S.5 41S.6 431.1 429.0 403.3 6 440.7 433.9 437.0 397.7 403.3 483.1 429.1 402.7 434.7 460.1 45S.0 417.2 7 458.9 453.1 4SI.3 42S.2 421.S 514.6 445.3 429.2 4SO.6 484.1 474.5 437.1 1 480.3 484.1 a 444.9 434.5 S37.2 471.S 442.6 502.it0 449.0 9 500.2 S03.6 $% 462.3 450.0 SSI.5 488.6 464.9 6 523.S 469.5 10 511.1 516.3 47S A 5 404 7 582.8 SOI.4 478.3 S39.3 498.8 11 520.5 530.8 483 3 479.4 599.1 S12.2 492.2 1% ssi.1 It 500.4 DEAD ANIMAL co AN114AL NUMBER GROUP 67604 67605 67606 67607 7601 :7609 07610 67611 0?612 67613 076614 sy is 14 is 3 MALES - 1$ 17 10,0000 PPM INDIVIDUAL BODY WETGS"Ts 13 WK DIETARY TOXICITY TUD Y(WGIT" T-5180 WEEKS Is 19 20 21 632 1 635.4 OS9.0 669.0 682.8 696.0 711.2 728.0 DEAD ANIMAL OD co AWTW NUMBER 0 1 GROUP 4 MALES - 67616 67617 67618 67619 67620 67621 67622 67623 67624 6762S 67626 67627 67628 67629 67630 67631 67632 01633 57634 67635 67836 67637 67638 67639 67040 67641 67642 07043 07644 6764S 67646 07647 67641 67649 67650 67651 67652 67653 67654 67655 67656 67657 67651 209 2 104.1 211.5 212.1 222.7 207.7 220.9 193.7 193.3 196.2 207.7 198,0 220.8 219.9 217.2 193.2 207.5 203.4 204.2 ISS.9 197.1 189.6 193.1 218.7 187.1 183.5 209.5 119.4 215.3 210 8 206.2 185.9 209.1 184.2 205.4 21S.7 204 9 201.6 198.7 212.2 195.0 21S.1 199.3 268.0 236.0 274 8 269.5 291.6 265.9 278.8 245.3 249.2 251,6 264.S 252.6 290.2 281.1 276.2 248.7 261.1 266.0 266.0 249.1 255.0 238.3 254.5 278.4 247.9 230.8 277.1 237.6 27S.4 279.0 264.7 238.6 265.1 230.3 267.0 277.2 259.3 257.6 254.4 267@7 254 5 270.8 25S.8 - DEAD ANIMAL 2 3 30.0000 PPM 220.9 277.6 328.6 248.7 335.0 313.4 323.0 293.5 296.3 218.2 253.6 300.0 322.2 337.0 331.3 2S4.5 301.6 290.9 309.1 206.2 293.1 282.0 303.2 320.7 296.3 279.7 333.4 210.2 320.2 331.8 313.4 281.1 313.8 285.1 322.2 327.7 295.7 318.8 292.1 313.7 30S,7 322.4 295.0 357 8 310.5 370.4 351.7 390.3 354.5 366.6 313.7 332.8 327.7 334.6 334.2 306 3 379.1 375.9 324.6 335.5 375.3 349.1 336.2 326.0 314.7 344.9 366.8 335.3 315.2 370.7 309.8 3SI.0 380.4 353@3 317.4 346.2 321.0 364@3 348.5 327.0 362.2 298.0 351.8 341.2 354.5 328.6 INDIVIDUAL BODY WE12HTS 13 WK DIETARY TOXICITY TUDY IWGITH T-5180 - WEYKS 4 5 6 7 8 9 393.1 330.4 403.6 385.4 429.7 303.1 400.2 340.0 301.0 35S.1 366.3 363.9 427.7 407.8 411.2 356.8 364.7 41S.4 379.0 367.9 353.3 343.7 375.9 395.0 365.0 364.9 441.0 303.3 407.0 447.3 311.4 340.2 373.0 3SI.1 397.9 377.2 347.8 401.5 320.3 384.4 378.8 392.7 348.8 427.3 362.2 442.3 ON 450 9 428.'5 427.0 386.1 390.2 317.0 316.1 451.6 438.1 451.7 450.9 386.1 466.4 451.0 443.4 3$S.4 414.4 a 40S.? 470.61 4S4.1 4SI.2 401.2 404.1 31.].0 3 .3 404.1 421.3 ON 371.1 433.S 3SS.4 409.1 463.0 414.0 374.6 391.03 422.S 403.6 373.4 439.1 413.4 381.3 422.7 369.8 425.8 427.3 A 381.9 430.8 &39.6 It 390.5 4SS.3 371.0 421.S 493.9 432.0 394.2 41S.62 446.7 414.5 394.8 467.9 436.A7 414.0 440.5 373.4 473.1 404.0 488.2 480.4 454.5 399.4 434.0 a 42S.9 488 A 1 471 4 454.S 4SI.5 390.1 400.4 461.0 410.08 471.5 317.9 442.6 516.2 457.6 419.7 426 & 8 467 2 449.'5 411.7 480.8 459.0 443.3 456.4 391.1 494.4 6 505.4 501.2 471.4 414.3 452.6 a 442.2 514 a 7 479.3 414 0 444.S a 404.7 k 540.6 441 & 9 489'3 474 3 440 4 508.3 a 410.4 SIS.4 A 529.2 524 1 481.2 425.4 408.6 0 447.7 528 1 i 495.1 428 9 485.1 a 416.9 'k 564.0 466 9 502.9 489 0 453,6 525.7 a 417.9 10 525.7 $38.09 540.0 410.5 432.7 475.5 459.a 1 540 6 500.0 432 4 471.4 422.0 ses.2 487.7 510.6 500.4 465.6 543.5 430.7 11 540. a 561. 560. 497. 446. 495. a 472. 563. 513. 440. 493.9 & 434.9 A 519.1 a 504.1 525.1 SIS.4 477.4 551.1 a 436.8 co %0 ANIMAL NUMBER GROUP 14 is 4 MALES - 67616 67617 61618 07619 1620 :7621 67622 67623 67624 6762S 67626 7627 6 7621 0?629 67630 67631 7632 667633 67634 67635 67636 67637 6?638 Sil.3 S17.9 481.6 a 504.4 502.S 601.2 a a a 0 414.9 482 0 6077664309 7641 :7642 67643 07644 67 5 67646 7647 57641 67649 7650 r6,7651 ,6?6S2 67653 676a54 7 ss 7650 667657 67658 539.4 11 S44,3 a S43.3 5S6 2 - DEAD ANIMAL to 17 30.0000 PPM S2$.3 537.1 SIS.4 a 614.8 520.4 a 630.9 494.6 508.4 S47.4 570 5 559.0 579.6 lt4DIVIDUAL BODY WE143HTS 13 WK DIETARY TOXICITY STUDY IWOIT" T-SISO 3 is 19 20 21 53$.S 547.2 SS3.0 sso.6 522.0 636.8 53O.S 041.a4 S47.1 ase.A9 Sil.5 523.4 SIO.1 556,4 582 1 573.4 595 9 580.4 602 6 i 592.3 611.3 13 INDIVIDUAL WK DIETARY TBOOXIDCYITWYEIGSHTTUSDY (OJT" W T_Silo NUMBER 0 1 OROUP 4 MALES - 670S2 67660 $1661 67662 67663 67664 O?68S 67666 67667 67661 67669 67610 20S@9 200.1 181.3 208,6 199.1 214.8 194 6 219@7 201 6 210,7 209.S 203.2 270.1 26S 0 24S.3 263.3 25S.7 269.5 249.1 276.6 2Sg.6 277.6 266.9 263.7 - DEAD ANIMAL 2 3 30.0000 PPM 314 1 311.4 214.6 308.2 306.2 307.8 299.0 319.8 318.2 331.S 31S.2 320.3 35S.6 349.0 317.0 337.4 346.5 354.7 335.7 357.0 360.S 370.S 355@3 362.2 4 390.9 379.0 344.1 364.7 381.0 392.1 36?.S 319.5 396.2 401.1 381.3 391.8 5 a 7 1 - 409 0 363.8 386.4 427 4 2 413.2 428.6 410.9 407.4 416.7 424 7 3$S'S 397*1 446 6 0 i 430.1 449.9 443.1 423.9 443.4 444 8 40S'l 413.1 473 0 4so.3 475.0 462.3 439.6 462.3 466.8 S04.7 a 471.6 a 472.2 462.3 479.7 9 478.0 524 3 419.8 a 416.1 470.S 487.4 10 489.0 543.4 0. 496.1 Soo 0 8 48S.S SOS.4 li 401.7 556.61 SIO.6 514.6 49$.S S20.9 ANIFIAL NUMBER GROUP 67659 $7660 67661 7662 :7663 7664 66?065 7606 86 7667 07668 67669 $7670 - 14 4 MALES a Sle.5 is - a 519,4 607.1 S49.1 621.1 541 3 550.1 560.3 - DEAD ANIMAL is 17 3D.0000 PPm 4 530.6 0. 521.1 634.6 563.& 4 $43.3 S72.45 571.1 512.4 INDIVIDUAL BODY WEIGHTS 10 13 WK DIETARY TOXICITY STUDY WITH T-5180 Is 539.a0 19 a 545.3 20 0 S4$.O 21 562.0 650.0 s7s.l 661.2 a 584.7 876.7 dk Soo.$ eos.s S91.4 608.3 611.2 lndividlatBody Weight Data (9) Animal Nupbgr C67671 C67672 C67673 C6?674 C67675 C67676 C67677 C6?678 C6?679 C67"0 C67681 C67682 C67683 C67684 C67685 C67656 C67687 C67688 C6?689 C6?6" C67691 C67692 C6?693 C6?694 C67695 C67696 C67697 C67698 C67699 C67700 C67701 C6?702 C67703 C67704 C67705 C67M C67707 week 3 -L -L -7 a 9 10 11 12 100 ppm 1-5180 214.8 246.8 291.9 313.3 349.9 388.2 410.5 429.0 453.5 474.7 491.0 507.8 524.1 54 219.0 265.6 307.7 348.2 375.6 401.3 431.8 457.1 471.8 491.5 499.0 466.0 469.2 464 193.4 244.2 287.6 321.0 355.2 382.3 406.4 434.0 439.4 452.7 462.9 471.3 4B5.6 49 225.0 279.9 316.1 356.0 385.5 422.0 442.9 467.9 492.0 513.2 520.1 535.3 546.2 55 198.8 258.5 309.6 349.2 382.9 a 6 211.5 271.5 318.6 353.2 385.6 414.3 437.3 45?.4 472.0 469.8 442.3 448.1 443.1 411 200.7 254.1 298.6 337.7 371.3 403.9 424.8 447.5 465.9 486.1 497.2 507.1 515.9 530 189.1 238.7 281.0 320.7 352.5 305.2 408.6 431.3 442.1 457.9 471.0 481.6 493.0 501 187.2 230.0 273.1 297.3 318.5 336.6 348.8 372.1 384.1 395.1 406.4 419.0 431.4 43 192.3 242.8 283.7 308.4 341.5 380.8 404.3 421.7 195.2 241.4 288.7 310.4 337.8 369.6 199.2 248.7 290.8 322.4 420.6 197.4 250.4 299.5 340.1 369.1 398.9 420.0 441.8 463.4 483.1 497.2 506.7 522.1 530 206.5 251.4 292.2 326.1 355.4 380.0 395.6 413.5 422.2 432.8 432.2 446.3 450.6 462 199.0 240.3 274.5 302.1 a a 199.7 258.8 300.2 351.8 384.7 420.0 445.2 462.9 475.9 4".4 509.9 527.8 53?.9 550 206.3 257.9 304.4 332.0 356.1 382.2 * .* 4 a 186.4 232.S 272.3 304.5 322.0 335.7 352.9 372.5 383.5 397.1 339.1 371.5 376.8 400 183.6 243.2 290.3 331.3 362.0 212.9 251.3 297.6 328.0 344.1 193.6 234.5 261.1 272.9 291.4 313.1 325.8 348.3 359.4 373.8 358.8 378.8 389.3 402 212.4 2" .0 310.2 340.7 376.9 206.5 256.1 307.0 346.2 377.1 191.5 236.8 273.0 310.3 337.6 36;.l 39;.l 41;.S 43;.9 44;.B 46;.B 4?;.4 49;.s 512* 185.6 226.1 254.5 280.2 300.9 331.6 344.7 360.3 359.2 362.7 369.4 376.5 376.7 386 216.3 264.4 294.7 314.6 334.6 t a t a 214.8 259.3 293.5 320.8 342.3 366.0 389.8 403.3 412.3 429.5 441.1 456.5 461.9 481 199.3 245.1 286.3 322.5 357.0 390.7 188.8 239.1 280.2 321.9 356.9 395.9 421.7 438.6 210.4 256.0 296.3 329.1 361.9 404.0 428.0 452.3 475.2 486.0 508.7 526.4 536.1 554 195.9 238.1 285.8 321.2 245.6 373.3 397.9 410.7 425.7 438.8 454.1 463.4 4T3.3 484 200.8 266.3 314.3 353.2 379.7 411.2 433.5 454.2 212.8 262.9 296.3 335.6 372.6 405.2 431.3 460.0 207.8 255.6 294.9 331.2 354.5 385.8 408.3 424.5 446.6 464.2 478.9 494.8 507.0 513 191.5 249.5 305.8 350.7 387.2 422.4 447.1 475.4 201.8 252.9 296.7 321.3 345.3 373.9 400.2 417.1 430.4 448.8 463.9 470.4 482.4 496 206.6 258.8 314.0 354.9 387.6 423.8 457.5 485.8 Dead animat Individual Body Weight Data Animal Number Cfi?671 C61612 C6?613 c6y674 Cc:i':7?'6' C6?67 C6?676 C67:79 C67 80 C67681 C6?682 C 7683 C67304 C67685 C6?6:67 E6y C67::98 c:@ C Y690 C61691 167692 C67693 C67694 C6769S C67696 C67697 C6,16989 Er 769 C67700 Cfi?7O0Z1 671 CC67703 C6YY 04 6?YO5 06 Cr671 ('6?70? 14 570 9 41; 4 50; 3 538 1 463 1 520 7 48; 2 5.55 9 so; i Week 15 16 17 Is 19 zo 100 PPm V-5180 (Continued) 58;.3 42;.4 51;,9 60;.l 42; 9 52;.3 61; 4 43; 0 53;.S 61; 2 43; 9 53; 6 63;.6 44;.S 54-0.8 64;.S 36;.B S4;.S 39; 3 S48 6 477.4 560.3 486,5 567.0 490,3 5?6 1 503@O 578.9 508.S 587.5 509.8 S03.2 53; 1 596.5 56; 8 565 9 578 1 58;.9 502 1 sio I SIR 1 577 2 538 S 543 3 @46 6 @l 0 558 9 52*7 3 51,2 11 1,11 11 1172 2 1147 2 582 1 550 9 S91 2 SS3 I S94 6 Dead atiima I Individuat Body Weight Data (g) Animt NuRber C67708 C67709 C6?710 C67711 C6?712 C6?713 C6?714 C6?715 C67716 C67717 C6?718 C6?719 C6?720 C67721 C67722 C6"23 C67724 C6?725 0 1 210.3 189.5 202.2 208.5 218.0 223.7 193.3 212.3 209.2 195.6 220.2 220.8 228.9 215.7 201.0 223.9 208.6 199.7 263.9 236.2 255.4 256.3 266.5 273.9 225.3 260.9 259.6 245.5 271.5 271.9 287.7 258.7 246.5 269.3 262.8 241.6 311.3 264.2 303.9 307.0 314.2 312.8 242.0 301.3 298.1 289.8 316.8 317.6 329.9 2".S 292.9 316.3 298.8 278.2 352.5 290.1 340.7 337.4 354.4 335.1 2".B 328.4 323.7 316.8 354.5 351.6 375.2 334.7 330.7 346.6 335.0 309.1 382.3 308.3 369.7 465.6 356.0 351.8 280.3 359.2 348.7 343.7 387.1 381.8 413.8 363.2 359.6 375.1 364.1 332.4 week -5 -L -L a 100 ppe T-5180 (Continued) 423.7 333.7 397.1 392.9 420.6 375.9 298.9 454.4 350.2 418.2 412.8 446.0 0 315.6 483.4 365.3 436.1 433.4 471.9 & 344.6 493.9 354.3 371.6 417.6 413.0 460.1 391.5 380.1 402.8 399.5 391.3 & 443.0 436.7 491.3 415.9 403.7 421.7 427.3 403.3 461.5 462.0 523.8 434.9 421.3 441.7 452.2 418.3 545.3 456.3 9 511.8 369.5 431.1 575.0 465.9 10 527.4 382.6 439.4 600.5 479.9 11 539.8 396.5 448.6 619.9 491.8 12 559.6 403.9 457.3 641.7 497.2 1 576. 417. It 469. 660. 508. Dead animi IndividualBody Weight Data (9) Animal NLpnbre C6??Oe C67709 C67710 C67711 C6?7112 Cb7? 3 C61114 C6??14 (6yyl1s c 77 176 (671 C617198 C671 C61?201 C61?2 C6172? E61?23 C61?245 C6?72 Week 14 16 19 -ZL -ZJL 100 Dom T-5180 IContinued) 512- .4 520.9 526 0 S40 0 S43.7 541-.7 ssi@l 558.1 Dead animal %0 Ch IndividualBody Weight Data (g) Animal 0-ber C6??26 C6?727 C67728 C67129 C67730 C6??31 C67732 C67733 C6??34 C6?735 C67736 C61737 C6?738 C6?739 C67740 C67741 C6?742 C67743 C6?744 C67745 C6?746 C67747 C67148 C6?749 C6?750 C6?751 C6?752 C67753 C6?754 C67755 C67?56 C67157 C677se C67759 C67760 C6?761 Week 4 -L -L -L -L 10 202.6 202.t 204.1 226.? 212.8 222.3 226.3 229.1 216.1 217.5 217.2 216.5 220.6 202.3 211.2 218.7 195.6 213.0 205.2 217.5 209.2 ZIS.8 225.6 217.4 ?16.4 210.9 224.0 223.9 220.1 213.8 195.1 223.2 217.3 216.3 190.4 189.1 249.0 249.9 244.S 2SY.2 251.8 266.6 261.9 259.1 259.1 252.1 255.6 256.1 267.1 249.2 243.4 261.1 241.3 258.6 252.1 258.9 251.5 263.6 262.1 251.1 261.7 254.2 264.1 264.6 263.3 249,6 242.7 264.4 260.6 258.1 238.8 240.4 283.9 294.6 2@S.4 290.6 291.7 306.9 297.2 298.6 301.3 294.2 298.5 293.3 303.2 295.0 276.6 299.7 282.1 299.9 291.6 301.4 295.8 303.4 303.9 294.7 298 6 296.8 305.6 302.0 306.1 288.2 286.7 305.1 303.0 299.1 280.9 278.6 323 2 338.4 323.3 326.2 331.3 343.2 333.7 331.1 338.7 326.8 339.5 330.3 345.8 337.8 308.6 339.3 322.5 337.9 331.0 341.5 339.? 342.1 343.5 329.9 334.5 33S.8 348.3 343.8 344.2 329.7 330.0 354.1 342.5 344.5 317.0 319.5 3S4.3 366.4 348.9 355.1 3SO.1 374.3 36S.0 364.8 362.0 356.0 371.9 367.6 380.2 368.4 337.3 3?1.4 354.6 362.7 363.1 315.8 369.1 370.4 375.4 360.2 365.1 363.2 300.9 373.3 377.0 360.6 360.6 318.9 317.9 374.3 346.1 353.0 0 Dom T-5180 378.2 399.8 382.7 403.7 400.8 .. 386.3 404.9 408.9 408.9 401.8 359.1 e & 392.3 394.1, a 395.6 408.1 405.0 391.4 393.0 39;.2 425.9 401-S 420.3 423.4 401.7 e 422.0 431.0 425.1 425.6 372.8 410.9 416.8 409.0 426.4 426.5 407.9 418.7 41;.4 449.0 421.9 438.6 446.8 A 412.6 435.8 464.1 440.8 451.9 440.1 449.3 389.3 458.5 6 459.8 -40S.4 428.3 433.0 422.9 45?.3 442.5 426.3 436.3 4SO-3 438.3 47?.0 457.5 441.6 417.1 413.1 410.6 386.8 368.4 6 404.9 6 3?3.9 441.1 437.6 434.3 406.6 408.7 423.1 394.3 469.6 460.0 452.4 414.3 429.1 439.4 413.7 473.8 427.4 440.7 6 437.5 450.0 401.7 a 473.5 477.3 6 427.7 465.1 t 446.4 489.1 476.2 460.2 496.0 445,4 456.0 6 454.2 451.1 490.0 416.1 a 488.7 0 435.4 469.6 440.2 493.8 479.1 46S.8 501.1 452.2 461.7 463.9 466.0 500.3 493.9 502.3 451.2 479.3 459.1 508.S 491.1 471.9 514.4 460.6 473.5 474@O 477.? 514.2 S04.4 510.7 461.4 489.2 460.1 S23.4 501.1 48S.6 526.0 411.8 484.5 491.0 13 491. 525. S]4. t S26. SOO.? 464.5 534.2 532.6 492.7 Dead animal IndividualBody Weight Data (9) Anim I Owbor C6?762 C67763 C6?164 C6??65 C6?766 C67161 C67168 C67169 C6?1?0 0 2 Week -L _L -L 10 11 12 0 oym 1-5180 f ontinued) 215.4 2S?. 3 297.9 340.2 367.8 a 222.6 204.0 269.3 243.0 313.0 286.2 356.8 326.4 391.1 359.2 421.0 444.9 6 467.4 223.1 211.4 307.4 339.4 36?.l 394.2 413.9 429.3 198.8 244.2 286.8 326.4 3SZ.5 376.8 396.0 389.9 207.3 249.2 264.7 32S.1 357.2 394.9 417.3 433.8 453.9 466.4 472.7 488.2 492.5 50 208.0 250.3 290.9 333.5 360.4 392.S 413.4 435.0 224.8 258.1 301.7 346.1 377.8 196.4 246.8 286.3 320.3 342.3 Dead animal to OD INDIVIDUAL FOOD CONSUMPT107UICIWK 13 WK DIETARY TOXICITY S DY WIIH T-5180 Alffmi NUMBER 1 2 3 4 GROUP I MALES - CONTROL 67451 61452 67453 67454 674SS 67456 $7457 67458 674S9 67460 8?461 67462 67463 67464 $746S 67466 67467 67468 67469 674?G 67471 67412 67473 67474 67475 67476 07477 67478 67479 674$G 67481 61482 67483 67484 67485 61486 67417 67488 67489 67490 67491 67492 67493 172.1 163.4 183.7 176.0 173.9 162.3 171.2 161.3 149.1 162.0 163.8 170.3 170.0 168.6 160.3 179.5 163.4 170.4 174.4 167.9 177.0 181.6 178.3 166.7 180.9 158.1 165.2 162.1 148.3 172.6 157.8 146.S 179.0 106.2 179.4 166.2 165.5 lss.7 172.8 171.2 ISY 1 188.4 148.8 lls.o 171.1 201.0 191.6 203.2 171.S 170.8 171.4 165.G 181.1 113.9 184.9 172.6 189.9 166.9 187.3 170.6 169.4 185.6 181.0 at 210.9 197.9 ies.i 191.4 175.1 182.1 174.9 166.4 190.6 166.3 155.1 187.7 176.9 196.1 172.1 173.4 197.0 168.4 186.2 163.6 193.2 ISO.@ 198.8 176.4 210,6 218.7 216.5 114.9 179.3 180.5 173.6 181.1 117.5 lol.S 175,7 igo.s 172.2 186.8 177.1 176.0 192.S 191.3 185.6 216.5 2G9.l 195.1 200.2 179.9 193.8 184.4 114.2 2GO.0 175.5 IOS.S 00 am 201 0 171.1 179.7 205.7 171.3 194.1 171.2 203.0 159.9 209.5 190.1 217.7 222.6 222.0 1$? 9 181.4 186.0 178.9 193.4 201.1 196,9 184.7 190.9 178.3 200.3 187.5 IIG.1 196.3 192.1 %* 214.7 216.6 198,4 213.5 188.8 201.3 193.0 182 1 206.6 179.1 173,1 183.0 201.2 215.1 184.1 179.5 214,2 178.3 *a 182.5 209.5 163.8 a DEAD ANIMAL - INVALID VALUE - FOOD WASTED OR SPILLED 5 213.8 194.6 221.2 225.5 221.4 189.3 117 6 4 186 7 loo 6 198.6 197.6 190.8 182.3 196.4 191.7 1% 204.2 194.2 215.5 199.0 202.1 208.3 195.2 204.0 196.3 1?1.1 211.2 187,9 179.S 192.7 206@4 224.1 189 1 217 6 211.4 163.9 WEEKS 7 6-. 190.9 211.2 189.5 21S.1 191.1 its I 114 1 191.1 199.0 204.5 176.5 192.3 200.1 Jos s 170.2 lto.s 19S.5 198.6 172.S 186.2 2G2*.a2 191.3 216'1 212.7 195.8 207.4 20G.4 194.5 206.3 201.2 216.1 199.2 206.1 193.1 169.6 202.0 181.7 170.3 119.2 203.0 222.8 117 a 9 216.2 191.0 192.9 162.9 191.2 170.4 172.0 187.0 198.4 219.S 113.A 8 204.6 a 193.6 164.8 162.2 8 184.1 214.4 193.0 109.0 179.1 198 9 193 0 202 0 210.0 197.2 14219 157.9 192.4 222 a 1 183.2 IOS.S 187.0 158.7 9 193.6 216.9 193.3 175.7 184.2 20S A 7 198 2 198 S 219.0 205 6 2 i 169.8 172 0 204.1 221 A 9 194.1 209.9 189.8 164.1 10 182.3 2Gg.6 188.0 171.2 170.3 201 1 190 6 IOS.6 209.4 9 206.6 16S.6 111 0 190.9 222@9 187 1 199.0 a 186.9 lso,l 11 ISO 4 205.S 188.5 168.9 174.4 194.6 119.0 202.3 199.8 121.3 164 2 182.S 22314 182 8 194 6 178.6 161.1 12 175.3 loo.7 182.1 170.7 173.8 a IOS.4 107.0 208.6 6 203.6 116.2 169.5 186.9 215.40 106.8 179.9 167 5 to to INDIVIDUAL FOOD CONSUMPTION 13 WK DIETARY TOXICITY STUDGY/WWK1114 T-5180 NUMBER is 15 17 GROUP 67451 67452 67453 674S4 67455 67456 $7457 67458 $7459 01460 67451 61462 07463 61464 57465 87466 67467 7468 667469 7470 687471 67472 57473 67474 6747S 67476 7477 : 74?8 67479 67480 67481 07412 67483 I MALES - CONTROL a 185.2 200.8 A 182.9 192.3 178.0 195.0 184.8 184.2 lss 5 188.6 187.S 184 5 187.9 157.2 180.1 173i2 172 0 176.1 617::45 67486 67487 7488 667489 67490 67491 67492 67403 174.7 165.0 177.2 ISS.0 182.6 163.1 - DEAD ANIMAL is 19 183.2 203.2 A 196.2 207 0 iss.1 202.3 197 4 189.6 181.5 139.3 179.3 177.6 181.2 227.4 190.0 167.1 WEEK5 20 21 A 115.6 210 1 201.0 A 198.0 194..S 203.2 143.2 186.7 171.2 IRS 1 i 0 183.2 A 112.3 NUMBER GROUP 67494 6749S 67496 67497 67491 67499 67500 67501 67502 67S03 67504 67sos 1 2 3 I MALES - CONTROL 160.5 197 7 ISI.0 156.4 174 9 169.9 166 0 IS3.9 a& 17?.6 178.4 169.1 163.0 210.2 172 3 171.6 ISS.7 189.2 176.6 163.1 A& 181.8 191.8 185.0 106.7 93.7 180.4 171 0 194's 201.S 177.1 171.0 198.0 192.1 199.1 IOS.S 4 171.7 && 176.9 114.3 199.4 208.7 196.1 180.3 A& IOS.4 203.0 200.0 INDIVIDUAL FOOD CONSUMPTION y JO/WKI _Silo 13 WK DIETARY TOXICITY STUD WITH WEEKS 5 6 7 8 9 10 171.8 P& 182.4 191.0 201.S 213.S 1% 181.9 212.1 203.0 174.8 && %a 114.0 201.9 212.4 176.&6 210.0 203.0 les.9 206.2 IIS.1 182.7 20S.6 209.5 172.&8 207.6 196.5 179.1 180.3 203.9 174.46 200.0 192.3 176@6 179.6 201 3 IIS.4 204,7 190.5 111 0 177.3 204.A3 a 167.8 206.8 193.4 11 185.3 177.2 194.4 a 168.1 200.4 194.0 12 176.8 170.9 194 6 7 161.4 197.8 198.1 DEAD ANIMAL INVALID VALUE - FOOD WASTED OR SPILLED ANIKAL NUMBER ONOUP 67494 674$S 61496 67407 7498 6 7499 67soo 67501 67S02 :75503 7 04 67505 is I MALES 16 17 - CONTROL INDIVIDUAL FOOD CONSUMPTIO I (WKI _Sl%o 13 WK DIETARY TOXICITY SIUDI Wol is 19 WEEKS 20 21 162.7 171.4 IS7.8 112.3 165 2 180.6 181.1 ISS*3 109.1 166*9 18$.S 192.2 DEAD ANIMAL INVALID VALUE FOOD WASTED OR SPILLED INDIVIDUAL FOOD CONSVMPTION 10@WHKI 13 WX DIETARY TOXICIT STUDY Wl H -5180 ANTM NUMBEft 1 2 4 ONOT)P 2 MALES - 1.0000 PPM 67506 $7507 67so8 67509 67510 67511 67512 7513 6 ?514 7515 : 7516 67517 elsis 67519 7S20 a67521 7S22 6O?S23 67S24 67S2S 67526 6YS27 87528 7529 :7530 67531 6?S32 67533 67534 67535 67536 67S37 67538 SIS39 67540 67541 67542 67543 67544 6754S 67546 67547 67S4$ 159.2 162.6 166.6 185.7 IS3.8 157.7 ISS.4 167.0 178.6 119.2 161.0 169.4 165.S 171.0 164.9 ise.o 168.7 181.3 163.6 166.5 Is$.$ 157.8 156.4 145.7 177.9 172.9 167.7 177.9 167.3 161.5 174.8 145.2 167.7 162.9 187.9 110.3 141.0 168.2 164.7 163.6 158.2 165.9 156.3 168.1 171.7 1?0.7 199.1 164.7 163.7 160.3 187.7 187.7 183.4 172.8 179.4 180.3 178.1 182.1 162.6 197.2 192.4 178.8 177.7 161.5 168.2 160.9 103.2 188.8 183.5 181.9 195.7 180.0 171.8 172.2 152.9 187.3 177.8 208.0 201.2 ISI.3 190.9 170.9 168.7 160.6 172.1 165.9 178.8 179.7 190.9 209.0 177.5 186.4 173.4 178.7 189.4 194.5 190.9 192.0 *4 193.1 202.7 177.3 216.9 202.5 189.5 191.9 174.2 181.1 167.7 169.8 202.8 194.3 192.7 201.? 191.2 190.5 178.6 163.0 Itik 19S.6 223.2 *4 165.1 205.7 179.6 175.1 166.0 173.6 172.6 182.1 191.4 195.5 221.0 lst.0 175.6 119.3 Ill.$ IOS.2 210.1 192.5 191.5 am 204.6 6* 180.8 219.2 208.9 202.3 205.1 179.1 112.5 180.5 179.3 215.2 210.2 201.3 201.0 193.7 196.2 186.5 169.0 202.0 198.6 230.1 206,7 170.4 213.3 Ill.? 180.9 173.7 181.8 182.3 - DEAD ANIMAL . INVALID VALUE - FOOD WASTED OR SPILLED 5 195.7 192.5 185.3 195.7 a a 190.2 197.4 oil% a^ lo;os 229.7 212.4 a It 187.9 193.7 0* a It 212.7 211.0 218.5 199.1 202.6 200.3 o 204.2 202.4 237.1 210.7 176.0 219.6 183.0 179.8 182.0 183.1 193.5 WEEKS 9 7 8 9 103.7 186.7 185.7 191.8 179.8 186 2 176 0 111 4 127 6 112.6 197.8 194.4 193.5 183.7 220.1 207.6 a 1:0.9 1 1.2 196.6 215.6 203.9 215.3 193.8 195.7 IOS.0 219.9 199.9 224.7 206.6 176.2 189.9 178.A4 180.1 182.5 183.5 178.8 196.6 194.9 193.0 20;77 113 2 184'9 230.S 210.9 175.3 186.8 me 211.1 204.6 204.8 194.4 203.3 192.1 202.9 223.3 200.3 170.7 180.6 111.5 174.3 179.7 178.7 184 2 191.4 193.8 19;73 183 9 177'3 207.3 181.6 173 9 203.6 192.6 200.6 187.9 190'1 200.9 114 6 172.9 IOS 7 194.5 194.9 20; -9 192 's 188's & 217.7 179.5 6: 204.9 192.6 202.9 199.4 202*3 217.5 180 A 4 182.2 10 185 9 112 0 197 6 187.6 113.2 19;74 les.1 168.6 a 204.0 178.2 168*3 i a 199.2 189.3 186.7 200.2 191&4 209.8 174 a 1 175.5 11 176.3 180.1 200.3 194.7 lls.s 175.5 186.S 211.1 189.6 16463 194.1 188.9 184.3 203.S 21701 201 9 163.3 I?S.S 12 173.9 173.2 200.2 185.1 187.6 17S.0 184.9 0 199.s 119,4 168*4 i 198.1 177.7 201.6 200.2 20761 202.1 a 149.1 171.3 INDIVIDUAL FOOD CONSUMPTION (G/WK 13 WK DIETARY TOXICITY STUDY WITH I-SLIO ANTOT NUMBER GROUP Is Is 2 MALES - olsog ISO? 6sisos 57sog 61sio 67sil SIS12 6?513 ISIA : 7515 $isle 61511 $Isis 7519 167S2D 07521 1522 :7523 67S24 67525 61526 7527 5 7528 67529 67S30 67531 67532 67533 7534 567535 67536 67531 67531 7S39 667S40 67541 158.9 152.9 202.0 19S.2 189.6 ITS.$ 193.0 194.9 188.1 ISS.2 151.7 154.0 iTy.4 167.2 IN a 207.5 203.1 67543 67544 67545 67546 67547 67S4# 150.3 180.6 172.6 174.5 a DEAD ANIMAL 11 Is 1.0000 PPM 161.2 112.9 200.2 190.1 187.1 151.2 210.9 201.7 195.6 169.3 156.4 167.5 162 1 113.t 204.9 213.4 149.5 206.5 ISS.2 190.4 19 173.7 203 6 200 5 203 3 159.1 166.4 172 1 i 216 3 111 6 196.7 WTEKS 20 21 LIO.? 212 9 223 4 194 3 166.0 174.8 LIO.2 ITL.4 161,1 164.6 173 t i a 217 5 150 4 195.1 20S.1 Cs>@ AWTW NUMBER GROUP 67549 6?SSO 67551 STS52 6?ss3 67SS4 sisss 67550 G?SSY 67558 67ss9 67s6o 1 2 2 MALES - 171.1 151.6 177@S 192.1 164.6 le4f*.5 159,S 177.7 171.8 181.4 173.1 171.1 162.1 190.2 200.S 169.1 19S.0 197.4 171.9 114.4 lli.s 203.9 183.0 3 4 1.0000 PP" IOS.4 177.4 202.7 193.S tol.i 211.2 203.9 175.0 195.2 ise.9 21S.0 108.4 216.3 179.1 208.7 2014.65 214.9 210.S 173.3 198.6 193.6 219.8 201.2 INDIVIDUAL FOOD CONSUMPTION JO(WHKI 13 WK DIETARY TOXICITY STUDY Wi H -5190 WEEKS 5 -6 7 8 9 10 220.9 186.4 201.6 211.3 197.1 21S.6 111 a 206 4 22S.0 207.3 211.1 adh 215.5 175 9 201.1 198.0 228.1 a 203.0 210.2 1%@% 214.5 17S.8 198.1 194.1 221.1 192.1 214.5 201.4 171.4 217.5 a 106.6 216.8 181.4 187.3 217.0 & 184.3 210.2 191.3 17S.7 11 216.4 112.3 214.5 IOS.S 172-9 12 221.0 6 182.2 193.6 184.2 177.6 DEAD ANIMAL INVALID VALUE - FOOD WASTED OR SPILLED mffwr HUMOER ONOUP 67549 7sso : 75si 67SS2 6ISS3 7554 6SYSSS 7556 607ssl 75519 66iss 67s6o Is is 2 MALES 17 is 1.0000 PPM INDIVIDUAL FOOD CONSUMPTION (G(WHKI 13 WK DIETARY TOXICITY STUDY Wl H -5180 WEEKS21 le 20 DEAD ANIMAL INDIVIDUAL FOOD CONSUMPTIO (0 w 13 WK DIETARY 7OXIC17Y STUD! WI(HI(I-sloo Nt)t4BER 1 2 3 4 GROUP 3 MALES - 10.0000 PPM $?Sol SIS@2 6?s63 67564 alses 67566 67ss? als6i 67569 sisio 67571 elSY2 67S73 67s74 67S?S 67576 67577 7S7: :7S? 67510 67sol 67512 67S$3 07584 57SB5 67see 7S$7 6 7ses 67589 elsoo 67591 67S92 $7593 67594 87SOS 07596 ,87597 67591 67s99 67600 67601 67602 67603 163.3 175.1 191.6 186.9 183.7 160.3 170.3 169.5 161.7 196.5 152.2 163.7 163.9 149.8 171.6 154.1 158.1 164.4 IS9.4 162.8 183.9 154.2 160.3 166.2 150.0 Adk 0.0 150.5 168.6 151.7 IS4.5 134.S 170.8 181.0 165.6 157.2 151.8 100.3 162.7 112.9 182.9 173.7 184.7 177.3 188.3 200.1 204@4 its.1 112.9 186.1 li?.l 171.0 200.0 171.6 170.4 168.3 163.4 188.6 jes.s 154.7 168.7 171.8 166@9 177.S 165.8 16$.2 179.7 172.9 181'.n3 163.3 17S.9 173.6 167.4 138.2 188.8 197.8 111.1 167.5 158.7 170.6 176.8 196.2 196.1 187.2 282.6 187.0 199.3 208.5 202.1 at 184.1 193.6 181.6 174.9 200.4 167.5 173.7 170.6 167.0 199.6 170.3 154.1 173.4 ISS.2 177.8 189.9 184.1 176.6 193.0 109.7 110.5 192.7 117.3 177.3 157.0 214.2 202.1 199.1 174.4 174.3 179.3 180.2 196.6 213.2 184.5 122.5 195.6 207.3 213.9 21?.S &,% ios.s 192.6 loo.4 as 212.S 174.0 181.1 180.7 174.S 204.9 170.3 ISO.$ 173.7 193.2 179.7 114.3 179.4 192.6 194.7 171.2 120.1 177.0 llt.7 143.7 223.0 201.5 200.7 171.2 ob 184.9 189.6 198.9 223.2 201.8 214.1 DEAD ANT14AL INVALID VALUE - FOOD WASTED OR SPILLED 5 200.4 201.8 214.4 214.0 210.7 a 208.5 210.0 177.1 181.4 190.3 179.0 213.4 114.6 200.0 114.7 1%4 179.S 195.0 210.4 20;.2 189.3 198.3 222.1 209.8 176.1 193 A 1 206.4 232.2 204.3 219.3 WEEKS a I 196.2 a 209.4 216.3 200.3 177.0 177.0 183.2 184.2 a 21S.2 184.4 200.1 too.5 196,3 173,1 188.5 211.0 203.0 100.4 203.7 191.7 a 20S.6 202.4 209.9 197.7 179.7 180.3 177.7 gk 221.1 179.9 193.3 110.4 200.7 177.4 182.5 213.1 203.2 20;43 177.1 191.2 222 7 209 9 119 7 205.0 h 200.0 208.4 224 S 209 1 176.1 194 A 3 212.1 h 194.8 218.7 1 189.1 203.48 200.4 202.1 112.2 171.6 187.8 161.2 1$2.4 215 6 i 161.6 110.7 199 1 210.8 9 192.8 213.a9 201.4 217.0 ito.s 111.5 173.9 196.9 IS4.0 184.5 212 4 i 175.66 A 194.6 200 A 4 208.5 10 192.0 201.1 205.S 211.4 110.1 169.3 120.0 167.4 171.8 207 6 171.8 185.6 208 2 199.1 11 ISO.S 207.00 204.4 19$.S 180.4 ISI.S 210'*7 183*9 171.3 ISI.0 217 3 171.7 6 188.2 209 2 199.1 12 187.2 208.0 192.$ 194,0 177.1 159.4 114*S 170.3 176.6 215 4 184.8 21S 4 6 205.2 ANiP4AL NUMBER GROUP 07561 67562 67SG3 67564 G?SSS 67566 67567 07568 67569 675?0 7571 :?S?2 7573 :7574 als7s 67576 7577 :7sll 67579 67510 67511 GIS$2 61583 7514 :?Sss 67586 67517 67sof 67589 G?590 67591 07592 67593 7S04 :7SOS 07596 ,e67S97 7591 7599 :7600 67601 67502 67603 Is 16 3 MALES - 192.5 192.1 17 le 10.0000 PPM 193.0 196.4 IOS.0 iti.2 171.0 178.8 110.5 172.6 a 210.7 1?0.5 108.2 202.a9 174.7 167.6 202 8 i 180.9 116.4 214 a 9 i lti.4 182.9 113.1 161.2 163.5 162.3 175.4 163.3 172.1 179.1 188.6 dk 200.5 212.1 207.0 216.8 DEAD ANIMAL INVALID VALUE FOOD WASTED OR SPILLED c@ co INDIVIDUAL FOOD CONSUMPTION (G/WK 13 WK DIETARY TOXICITY STUDY WITH 1-5180 WEEK5 19 20 zi 195.1 184.5 102.5 186.3 184.2 IYS.? 14S.3 215 7 i 171.4 176.3 223 a 0 i 186.2 toy's 179.8 lti.1 1?7.7 117.1 171.0 179.1 177.1 217.2 200.4 AIUW NLPMOER GROUP 67604 6760S 67606 67607 67001 67609 67610 S7611 67612 07613 67014 67615 1 3 MALES - 17S.8 174.9 IS4.8 167,1 156.9 203 8 163.1 ISS.1 149.4 171.2 170.5 171.0 112am 0 172.1 170.6 170@2 213.1 176.0 173.1 158.4 188.2 114.5 174.2 3 4 10.0000 PPM 1880.4 3 178.7 179.5 167.1 225.9 190.9 171.7 174.0 20S.2 187.9 195.6 193.8 185.6 114.8 176.5 226.2 196.4 181.3 116.0 201.1 191.6 177.9 INDIVIDUAL FOOD CONSUMPTION (G/WK 13 WK DIETARY TOXICITY STUDY WITH 1-5180 ----VM5 5 6 7 1 9 10 11 198.4 201.2 192.1 193.S 173.2 223.S 200.$ &ib 192.9 212.6 193.3 176.0 187.7 193.4 182.6 ist.6 170.7 225.1 190.9 174.6 116.7 210.5 194.5 1?4.3 lie.7 190.0 183.6 191.0 173.2 218.1 195.0 177.1 189.2 200.9 192.3 179.5 107.9 188.2 119.4 4 166.6 203.6 192.3 175.3 202 7 172.2 196.3 191.S 00 195.8 170.2 216.8 196.2 180.1 208.0 a 177.1 186.8 llgi.k S 115.0 114.0 221.S iti@3 179.a2 209.4 111.1 113.6 113.2 111.1 168.9 211.4 184.1 174.2 it 201.0 a 181.2 12 185.4 114.7 112.0 103.6 206.6 182.1 178.0 a 202.8 183.9 DEAD ANIMAL INVALID VALUE - FOOD WASTED OR SPILLED ANTWU NUMBER GROUP 07604 67605 67606 6776087 67609 67610 67611 67612 07613 07614 676is 15 le 3 MALES - 17 is 10.0000 PPM INDIVIDUAL FOOD CONSUMPTION (G/WK 13 WK DIETARY TOXICITY STUDY WITH 1-5180 WEEKS 19 20 21 193.6 les.1 203.2 205.1 210.9 215.9 217.1 DEAD ANIMAL INDIVIDUAL FOOD CONSUMPTION JO/WK 13 WK DIETARY TOXICITY STUDY W17H I-SISO NUMBER GROUP sisto 67617 $7611 6?619 67620 07621 07622 07623 07024 762S :7626 67627 67628 67629 67630 67631 67632 7033 :7634 07635 67636 67637 07638 67639 67640 67641 67642 67643 67644 6764S 67646 67647 87848 67649 7650 6676st 676S2 61653 7654 667ess 67656 6les? 616st 1 2 4 MALES - 175.4 151.1 172.1 173.4 20S.3 171.7 170.7 IS9,7 159.9 109.9 167.2 ISS.2 102.2 lso.i isi.0 153.8 158.4 108.0 IOI.S 111.0 lse.s 152@6 171.0 174.8 165.7 too@s 183.0 160.5 177.2 182.6 172.6 147.9 169.9 177.3 110.0 159.S 153.3 IS2.2 IGS.6 171.7 168.6 160.4 188.5 160.7 194.1 177.8 210.5 230.3 187.3 169.0 170.0 *& 108.7 167.2 231.2 187.5 193.2 lel.S ies.o 180.7 2S9.6 172.6 180.5 160.9 176.5 110.4 110.4 189.6 195.4 IST@2 183.3 192.9 181.9 142.1 180.4 172.2 190.9 271.3 163.2 177.6 158.7 Ill.? 186.7 172.0 242.0 3 4 30.0000 PPM 193.3 161.6 218.3 184.1 223.6 207.6 190.4 202.0 182.7 A& 204.5 230.5 21S.6 195.1 17;83 IIS.3 170.4 175.8 202.3 80.8 208.4 106.3 177.9 13.2 194.8 182.7 164.7 170.7 193.9 206.8 189.9 181.6 201.4 44 110.3 218.3 191.8 166.0 105.8 am 198@l Its,? 168@6 182,9 28.9 117.0 187.1 L89.4 166.1 lo;*i 190.2 174.4 181.6 202.3 190.8 222.0 les.9 178.0 215.7 IOS.6 186.2 170.0 174.5 207.S %* 190.0 190-S 114.6 139.5 ISI.4 202.1 200.4 172.6 190.5 206@S 202.1 204@4 163.4 194.8 151.7 204.0 191.0 201.8 167.0 o DEAD ANIMAL o INVALID VALUE - FOOD WASTED OR SPILLED 5 20S.1 182 5 1%@ 249.5 219.0 203.0 181.1 101.1 a& am ik 211.0 194.7 226.9 a a 222.0 202.3 200.0 lie.0 113.1 212.1 215.5 a 222.1 246.1 *ik 229.4 251.3 ah 174.7 A 210.4 203.2 200.9 168.7 61% ok 203@7 174.2 20S.3 172.6 WEEKS 6 7 205.6 to$ S 207.1 114 8 21S.1 197.7 112.4 191.1 .1 177.5 a 209.3 193.3 215.6 197.1 114.6 185.4 a 177.1 a 201.S 201.1 200.1 203.7 .6 179.6 213.9 216.0 a IBS.2 205.8 150.1 101.3 228.9 so 174.6 A 242.2 206.6 194.3 171.0 to gk 202.2 184.0 201.8 ISS.4 202.? 201.7 0. 171.9 227.9 *ik a 194.4 205-S 159.6 116.6 225.2 201.5 173.3 A *A 207.9 200.8 17$.S 207.1 0 198.4 194,1 toe's 160's 8 203.0 210,7 190.7 all 182.3 172.2 197 i2 197.4 111 2 190.3 6 153.7 220 7 173.1 203.1 191.0 174@9 197.8 163.1 9 204 9 215.0 189.6 ISS.2 115.1 k 170.6 204.a3 107.2 180 6 7 i 203.2 ISS.2 22O.hS 182.6 204.4 204.1 174.5 208.8 166.4 10 11 12 206 a 209 3 200.8 209 9 200.0 206.3 115.3 Okok 176.8 a ISS.4 It 200.0 209.8 167.3 ll;*4 174.S a 200.2 200.4 112.0 ll;*9 i 168.1 % 191.5 110.7 ISS.4 a llo.s 110.2 A 173.9 196.7 A IS4.1 190.1 a 193.0 203.0 198.9 172.4 202.6 too 1 154.8 0. 217.3 195.4 ISS 7 i 207.8 190.1 201.3 194.4 170.2 198.0 189.5 203.2 114.4 171.2 201.7 165.8 164.6 ISI.1 ANIMAL NUMBER GROUP sisis Is to 4 14ALES - IT to 30.0000 PPM INDIVIDUAL FOOD CONSUMPTIOI JO(WKI _Stlo 13 WK DIETARY TOXICITY STUD Wt WEEKS 19 20 21 7620 : 7621 67922 07623 67624 07625 67626 07021 57628 7629 :7630 7631 6 7632 67833 97534 6763S 67630 07631 07638 7639 867640 07541 67642 67643 6?68:4 67 5 7646 6 7047 67043 67649 67650 07651 ?SS2 I67053 7654 6 7655 57656 7657 607858 IGS.1 160.2 172.0 162.2 169.6 a 17$.S 170 3 i ISS.4 111 1 196.0 176 i1 191.4 177. 4 168.2 126.2 175.8 189.a3 192 9 190.0 177.9 194 3 i DEAD ANIMAL INVALID VALUE - FOOD WASTED OR SPILLED IGS.9 190 a i 190.7 196.2 191 5 lso.3 t$4 t i 147.0 196.8 188.&4 tl3.7 % 206.4 191.02 INDIVIDUAL FOOD CONSUMPTION IQ/WK 13 WK DIETARY TOXICITY STUDY WITH 1-5180 NUMBER 1 2 GRDK)P 4 MALES - 87639 67660 $7661 67662 67563 67664 67065 aloos $7087 Glass a7e69 al6yo 177.1 169.6 isi.s 172.1 ISS.9 173.1 IS2.6 110.5 IGS.? 112.0 169.2 167.3 110.1 175.9 ISS.5 171.1 IIS.5 173.3 167.0 181.2 179.9 196.7 170.$ 188.4 DEAD ANIMAL 4 30.0000 PPM 191.S 114.1 163.0 182.2 172.2 184.6 173.9 186-S 187@7 109.8 17S.0 199.2 201.0 ise.5 ISS-0 187.2 193.6 192.7 179.S 192.4 19S.3 205.1 194.0 103.1 5 192.6 167.7 187.0 200.2 1% 198.7 196.9 211.9 20S.3 200.3 WEEKS 6 7 jts.4 162.7 180.a4 192.&9 lse.7 I$S.t 174.5 193.7 201.3 113.5 ISS-0 171.it0 191.4 A 102.4 196.7 112.0 181.2 202,2 a 9 isi.9 187.5 195.a8 114.7 1?9 3 117 0 190.3 196.S a 196.3 174 6 1 19S.'2 196.7 179.0 124.0 a 115.6 176@9 loo.1 192.9 it 173.S 194.2 A 112.7 17617 194.6 191.8 12 172.5 192.8 A 185.8 153.0 116.7 190.6 INDIVIDUAL FOOD CONSUMPTION (GIWKI 13 WK DIETARY TOXICITY STUDY WITH T-5180 ER GROUP 67659 sleso flool 07662 67663 67604 fiess 67866 else? yesI : 7869 67670 Is 16 4 MALES - a 168.3 171.00 193.1 a 170.0 197.9 a 189.6 loo.5 191.1 - DEAD ANIMAL 17 Is 30.0000 PPM 171 i 1 113 0 a 191.5 0. loo.s 199.0 6 194.5 is$.$ 199.3 WEEKS 19 go 21 III 1 i 171.6 0 179.4 209.1 204.9 204 s i 197.5 203.4 198.4 192.1 IndividualFood ConstmptionData (9) Animi Nui*er C67671 C67672 C6?673 C6?674 C67675 C67676 C6?677 C6?678 c6?679 C6?680 c67681 C67682 C67683 c67684 C6?685 C67666 C67687 C6?688 C6?689 C6?690 C67691 C6?692 C67693 C67694 C67695 C67696 C67697 C6?6" C6?6" C67700 C6?701 C67702 C6?703 C67704 C67705 C67M C67707 1 136.9 148.2 141.5 163.9 159.5 168.0 148.3 144.4 150.2 139.5 155.6 146.2 153.5 153.2 146.1 160.5 154.6 141.0 152.7 155.0 147.4 167.5 154.3 155.5 138.7 154.1 160.6 145.8 161.8 163.0 151.3 170.0 168.4 159.5 156.4 141.4 167.4 153.8 158.8 156.4 169.5 183.4 180.9 161.3 154.4 163.2 149.0 175.9 162.7 1".9 166.8 150.2 166.6 171.4 154.8 163.5 161.2 143.7 1?6.8 1".9 162.5 141.2 157.4 167.1 153.9 171.3 16?.? 161.9 175.8 175.0 170.5 174.6 154.2 180.2 164.1 174.3 1".l 1".l 193.2 1".4 117.2 160.0 170.8 158.0 182.4 175.8 173.5 1?9.9 153.3 188.9 172.6 178.1 179.2 176.0 137.4 186.7 184.3 181.1 155.0 166.9 160.2 170.2 186.5 187.3 169.9 183.1 193.4 200.7 196.0 157.9 197.6 172.6 178.4 181.1 1".7 200.1 200.4 160.1 176.6 1?9.8 169.9 180.4 186.7 IDI.? 163.5 1".4 179.2 177.0 IBY.9 175.0 155.6 197.1 193.5 181.0 157.8 170.1 162.8 184.S 205.0 195.5 174.5 186.1 207.0 194.0 202.8 164.8 205.4 6 198.3 186.7 192.5 206.0 207.6 213.7 189.3 182.5 178.8 ISS.2 183.0 e 180.9 189.0 1".2 164.9 174.8 189.3 185.4 188.4 201.4 & 211.0 166.7 182.2 173.7 179.6 175.9 183.4 e 194.6 182.2 159.9 207.7 195.2 1" .8 167.? 171.8 191.9 195.6 219.2 209.0 103.6 191.5 221.1 204.2 209.3 176.8 202.4 ISS.S 186.8 162.8 189.6 215.1 204.9 182.9 190.0 236.3 198.2 204.2 176.3 213.3 Week 7 -L 100 mm 1-51 192.8 184.5 197.3 1".2 0 207.5 186.2 177.5 178.7 179.5 188.2 174.1 160.2 200.4 187.8 184.3 170.9 172.3 180.0 181.8 186.7 ln.9 1?6.8 178.4 182.9 a 169.1 159.3 159.2 189.1 156.0 e 186.5 e 196.8 201.6 173.1 188.2 215.3 1".7 206.5 170.2 210.8 180.3 148.7 0 1?9.8 189.4 1".4 168.5 157.5 185.3 195.0 t?2.0 162.5 149.6 -L 190.2 182.7 181.5 205.0 166.6 191.7 172.4 183.5 IT7.9 169.9 189.1 * 102.3 165.4 IW.9 147.0 It 188.4 2M.8 178.0 207.5 171.5 10 193.4 177.5 IT7.2 189.3 123.2 179.6 168.7 176.3 173.8 185.6 128.5 137.7 187.1 148.7 184.9 1".4 1?6.9 205.2 173.0 12 13 1".l 126.0 176.9 194.3 136.2 174.8 165.9 17'3.1 200.1 144.7 185.2 195.8 6 142.0 176.3 165.2 178.8 192.8 127.9 176.1 190.9 t 114.8 173.7 155.6 170.5 167.2 159.6 & 181.9 134.4 & 174.2 162.1 185.3 156.4 1".a 155.4 e 184.S 153.3 142.1 150.9 151.2 162.8 146.4 1".? 1".T 176.7 186.0 147.9 6 188.5 203.6 179.2 184.5 142.0 e 183.2 6 e 197.6 170.6 203.7 a 164.9 1".S & 1".1 187.4 167.8 Deed animal Invalid value - food wasted or spitted. r%b Ln Individual Food Consumption Oat& Animal 11to6or CGIGII C67612 c 7673 C:7674 E:7:15 c 1 16 C6?617 C67678 C6?679 C61680 C67681 C6?682 C61683 C67604 C6?665 C6?686 C57687 C61688 C6?689 C 1690 C:?691 C6769Z C67693 C6?694 C6762S C6?696 C6?691 C6?698 C6?699 c 7700 C:1?01 C67702 C6?703 C6?704 C6??OS C6?706 C6?107 -week 14 16 17 18 19 _LL _LL 100 ow 1-5100 (Contlnuedi 180.8 114.6 a 153.5 192.1 0 140.6 IS9.2 192.1 132.2 & 158.7 196.1 140.4 157.5 204.1 144.1 164.5 201.1 IS4.3 & 157.2 195.S 0 63.3 154.6 196.4 165.5 IS9.5 165.7 161.8 114.2 lez.s 167.5 177.6 162.6 171.0 17;.0 178.4 173.9 173.4 164.7 165.4 171.6 146.6 174.2 165.6 187.2 189.3 189.5 195.1 193.9 193.5 169.9 182.4 175.7 170.8 183.1 102.4 177.1 113.9 203.7 a 164.5 212.2 17;.S 217.0 201.1 ll;.9 16;.S 190.9 17;.5 196.7 16;.B 200.1 16;.S 192.9 17;.4 Dead animal IndividualFood ConsuRption Data (9) Animi Nu*er C67MB C6?709 C67710 C6"11 C67712 C67713 C67?14 C6?115 C6?716 C67717 c67718 C6?719 C6?720 C67721 C67722 C6?723 C67724 C67725 165.6 142.6 160.7 157.5 159.0 169.9 136.7 154.7 159.4 152.4 1".0 161.3 182.5 154.2 149.4 163.0 158.4 149.2 175.4 ISS.6 1?9.7 1?6.2 169.8 180.9 135.5 163.? 161.9 162.7 176.5 164.Y 195.5 148.0 161.0 173.4 163.9 1".o 196.6 1?5.8 203.1 178.1 1".S 192.6 140.7 ITT.1 165.4 1".8 193.6 180.0 224.0 171.9 176.3 166.4 191.7 189.0 201.2 170.8 200.4 181.0 19?.6 193.4 134.9 193.1 169.6 176.5 199.1 181.8 235.4 171.2 164.0 166.3 19?.6 lae.9 210.9 181.9 206.5 185.9 183.8 1".9 153.4 200.9 176.1 205.2 194.9 25?.0 187.4 176.1 194.3 211.3 Week -L -L -L 10 100 Use T-5180 (Contimied) 214.5 177.6 205.6 181.9 210.2 153.4 167.7 204.0 191.1 244.2 179.3 180.9 IN-0 208.9 220. 1 168.2 196.7 178.2 206.5 154.7 e 1".2 197.0 189.3 238.1 170.6 180.0 183.0 208.0 190.6 118.7 193.4 179.2 207.1 155.5 ol 160.8 & 201.6 136.1 242.0 145.1 129.6 IY6.6 1?5.9 215.7 a 158.3 165.0 242.9 177.1 212.5 160.1 t 162.1 243.4 172.1 205.5 159.0 138.4 244.8 173.8 213.2 158.6 & 160.7 240.6 170.3 218.5 157.0 162.0 236.2 174.3 Dead anioat Invalid value food wasted or spitted. Animi Nupgber C67706 C67M C67710 C67?11 C67?12 C6?713 C67714 C6?715 C67716 C6771? C6?718 C67719 c6rm c6ml C67722 C6?723 C6?724 C67M Individual Food ConstmiptfonDate (9) Wftk 14 15 16 17 18 19 20 1 100 wo T-5180 (Continued) 159.9 isi.3 179.2 170.1 1?9.2 173.7 171.5 li?.4 Deed animi Invalid value - food wastod or spitted. co lndividtoaFlood ConsumptionData (9) Animi C61726 CGI?21 Csl?28 C6?729 C6?730 C6?731 CG?732 C67733 C67734 C6?735 C6?736 C67737 C6?138 CS?739 C6?140 C6?741 C6?742 C67143 CG?144 C6??45 C67746 C67141 C67148 CG?749 C6?150 C6??Si C6?152 C6?753 C67IS4 C6?755 c677s6 C67IS7 C67758 C6?7SS C67760 - Week -L -L -L 0 pm 7-5180 jS3.0 IS4.9 154.6 154.9 154.1 154.9 154.9 152.6 IS3.0 154.? 152.9 154@3 IS3.0 153.9 154.9 154.9 153.4 ISI.9 IS4.9 154.9 IS4@9 IS4.9 IS4.9 154.9 154.9 154.9 154.9 154.9 154.9 154.9 150.4 154.9 154.9 154.9 152.9 165.0 165.0 16S.0 165.0 165.0 16S.0 165.0 16S.0 165.0 165.0 165.0 165.0 16S.0 165.0 165.0 165.0 IGS.0 165.0 16S.0 16S.0 165.0 16S.0 16S.0 16S.0 165.0 165.0 165.0 16S.0 165.0 165.0 162.8 165.0 165.0 165.0 160.0 179.3 112.3 179.3 179.3 176.3 179.3 179.3 1?9.3 179.3 179.3 119.3 119.3 179.3 119.3 179.3 119.3 179.3 178.6 179.3 179.3 179.3 119.3 179.3 179.3 1?9.3 179.0 1?9.3 119.3 119.3 179.3 176.7 119.3 179.3 179.3 166.0 184.6 104 @9 104.9 154.2 182.6 184.5 104.9 184.4 180.0 184.9 184.7 184.9 184.9 164.9 184.9 184.9 184.9 170.9 164.9 164.9 184.9 184.9 184.9 164.7 184.9 184.4 184.9 184.5 164.9 175.3 181.2 184.9 184.9 164.9 176.0 193.7 193.7 190-S 193.1 193.7 162.2 193.7 193.7 179.6 193.7 154.4 180,0 166.6 193.7 193.1 193,7 193.0 193.7 0 193.? 193.0 123.5 166.4 183.4 a 193.7 e 179.9 190.8 190.8 190.8 174.7 190.8 181.1 163.? 1".B 150.1 175.9 175.6 1. 190.8 190.8 190.8 190@8 172.6 a 190.8 190.0 ob 161.0 176.0 190.8 188.6 158.6 185.2 188.6 100.6 166.2 188.6 IYY.6 158.0 180.6 ISS.9 160-9 161.0 0 18e.6 188.0 188.6 181.6 163.7 t 108.6 181.3 184.9 153.0 175.1 187.5 il;.6 10;.$ 0 184.3 182.5 164.0 A 164.0- 176.4 e 154.2 183.8 183.6 183.8 162.4 156.9 170.3 I?;.? 104.8 6 185.1 IBS-1 & 165.9 117.3 181.6 185.1 183.6 ISS-1 184@o IBS.1 155.7 176.3 le;.o 10 17;.$ & IIS.9 176@i 160.3 166.3 171.9 175.9 174.9 176.1 175.8 116@l 159 3 161.8 17;.S 173.1 172.9 [6e.9 0 112.4 113.4 113.0 1?3.4 173.4 112.5 173.4 160.1 165.9 16-0.7 117.0 177.1 158.0 a 161.3 175@2 177.0 174.9 1??.2 fl?.l 177.2 155.8 164.3 ll;.2 171.3 171.7 & 15?.S 168.1 171.2 163.4 171.7 0 ISS.5 Dead animal. Spilled feed. IndividualFood ConsumptionData (9) AnlwAl 2 _a_ 4 -L vft* -Z- JL -@L -IL -iL AL 0pom1-5180(Continued) C67761 148.8 165.0 179.3 184.9 C67762 154.9 16S.0 1?9.3 184.9 C67763 154.9 165.0 179.3 184.7 1931 190.8 & 188.5 C67764 143.9 16S.0 179.3 184.9 C6??6S 154.9 165.0 IS4.7 169.5 179.5 66 164.7 C67766 154.9 165.0 119.3 160.0 183.9 168.5 153.6 E67767 149.2 165.0 119.3 184.9 192.9 164.9 179.3 176.1 177.9 110.2 161.7 163.2 161.9 C67168 IS4.9 16S.0 179.3 184.9 193.7 190.8 182.S C6?769 154.9 164.9 179.3 164.9 C67770 IS3.0 163.3 166.8 169.6 Dead anim 1. Spilled feed. HWI 6329-100 APPENDIX C IndividualClinical Chemistry Values 221 ANIMAL NUMBER INDIVIDUAL CLINICAL CHEMISTRY MALES WEEK S PCOAO IU/0 DATA GROUP: I C$7461 C67461 C67486 C67491 CBYSOO MEAN S.D. N GROUP: 2 C67524 C67S2S C$7530 C075ss C67558 MEAN S.D. N GROUP: 3 CS?576 CG?577 C$7591 colsos C67599 MEAN S.D. N DOSE LEVEL: 0 9 8 0 I I I s s DOSE LEVEL: I a 9 a 10 12 9 1.7 s DOSE LEVEL: 10 11 is to 17 is 14 3.6 s DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: PPM INDIVIDUAL CLINICAL CHEMISTRY DATA MALES WEEK 5 ANIMAL NUMBER ------ PCOAO IU/0 -------- GROUP: 4 DOSE LEVEL: 30 DOSAGE UNIT: PPM C$7627 is CG?631 21 C$7632 43 C67603 Is C0766S 27 MEAN S.D. N 24 11.4 s GROUP: S DOSE LEVEL: 100 DOSAGE UNIT: PPM C$7602 54 C67619 28 C07690 41 Col?l? 43 C6772S Is MEAN S.D. 37 14.6 GROUP: 0 C67726 CS?733 C$7741 C67745 CB?762 KEAN S.D. N DOSE LEVEL: 0 9 I I I I I .4 s DOSAGE UNIT: PPM ANIMAL NT#OER INDIVIDUAL CLINICAT CHIMISTOY MALES WEEK I PCOAO IU/0 DATA GROUP: I C$7493 CG?480 Co?4$1 CG?404 Co?495 MEAN S.D. GROUP: 2 colszz cols3i ClIS32 Co?540 ce7soo MEAN S.D. n GROUP: 3 colsis C$7513 COYS84 celsil CG?612 "[AN S.D. DO$[ LEVEL: 0 9 s 7 4 7 I I.s s DOSE LEVEL: I 7 1 I 4 8 I .8 5 DOSE LEVEL: 10 to is 2S 14 Is is S.5 s DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: FFM INDIVIDUAL CLINICAL C"EHISTITYDATA HALES WEEK I AN114AL NUMBER ------ PCOAO IU/0 -------- GROUP- 4 DOSE LEVEL: 30 DOSAGE UNIT: PP14 CO?634 3$ Col$42 32 co?444 30 colosi 49 C$7661 Is MEAN S.D. 32 12.2 GROUP: S C*7480 C67707 cslyoo C$7719 CS?722 MEAN S.D. N GROUP: I C$7731 C$7134 C67737 C07760 C67761 MEAN S.D. DOSE LEVEL: 100 ss 8 107 46 S3 S4 ..3S.3 s DOSE LEVEL:0 9 7 5 I I.S s DOSAGE UNIT: PPM DOSAGE UNIT: PPM 4Ln ANIMAL "UMBER INDIVIDUAL CLINICAL CHEMISTRY MALES WEEK 14 PCOAO IU/0 DATA GROUP: 1 C$7460 C$7462 C67473 C67417 co7sol MEAN S.D. N GROUP: 2 C67S2$ COYS33 COIS4$ cs7ssl coiSS3 MEAN S.D. N GROUP: 3 c67s64 C$7608 colsio C$7611 C670is MEAN S.D. N DOSE LEVEL: 0 6 7 s 7 7 4 .0 s DOSE LEVEL: I it 7 6 7 0 I 1.9 s DOSE LEVEL: 10 8 13 8 10 9 10 2.1 s DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: PPM ANIMAL NUMBER INDIVIDUAL CLINICAL CHEMISTRY MALES WEEK 14 PCOAO IU/0 DATA GROUP: 4 C$7616 Col$23 c67643 CS?652 ceiGS3 MEAN S.D. GROUP: 5 C67671 C07672 Col$91 C6?700 C$7714 MEAN S.D. N GROUP: 6 C$7730 C@7738 call4e C67754 C67767 MEAN S.D. DOSE DOSE DOSE LEVEL: 30 11 Is le 10 Is 14 3.4 s LEVEL: 100 to 14 12 20 23 IT .4.S 'S LEVEL: 0 7 6 s s 4 5 1.1 5 DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: PPM ANIMAL NUMBER INDIVIDUAL CLINICAL CHEMISTRY MALES WEEK 22 PCOAO ZU/0 DATA GROUP: I C$7453 C674se C97483 CG?490 C$7496 MEAN S.D. n GROUP: 2 Cf?508 ce7sto C@7534 C67538 c6?s43 MEAN S.D. GROUP: 3 C67567 CG?5?S C$7579 celSO9 C67593 14EAN S.D. N DOSE LEVEL- 0 6 7 7 7 8 I s DOSE LEVEL: I 7 7 10 0 6 7 1.6 ..s DOSE LEVEL: 10 a 7 7 7 5 I 1.1 s DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: PPM OD INDIVIDUAL CLINICAL CHEMISTRY 14ALES WEEK 22 ANIMAL NUMBER ------ PCOAO IU/(k -------- DATA GROUP: 4 Cf?626 CB?628 CO?637 Cl?664 cole7o MEAN S.D. N GROUP: S C87674 C$7671 cey::3 C61 4 COYTOI MEAN S.D. DOSE LEVEL: 30 9 6 7 1 7 7 1.1 s DOSE LEVEL: 100 7 a I 7 I I .4 ..s DOSAGE UNIT: PPM DOSAGE UNIT: PPM '"HAZLETON W W IS C 0 N S IN POST OFFICE BOX 75AS MADISON. WISCONSIN 53707-7545 Sponsor:. 3M St. Paul, Minnesota a CORPdW410 Lowaio,@ Sewes Ce-ma@v FINAL REPORT Study Title: 13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate(CAS No. 3825-26-1) in Male Rats Data Recuirement: Guideline 82-1 Autho : Matthew J. Palazzolo, PhD, DAST Date: May 17, 1993 Performing Laboratory: Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704 Laboratory-ProlectIdenufication: HWI 6329-100 VOLUME 11 OF 11 Page 230 of 657 P h0 ne 608-241-4471 FOR EXPRESS-k4AIL DELivEFtv 3301 KINSMAN 8OULEVARD Fa x 608 PAADISON. WISCONSIN 2A 1 72 7 7 53 704 CONTENTS VOLUME 11 OF 11 APPENDIX D IndividualAbsolute Organ Weight Data (9) Individual Organ-to-Body Weighttp;rcentages IndividualOrgan-to-Brain Weigh atios IndividualAnimal Pathology Data APPENDIX E Quality Assurance Statement IndividualHormone Analysis Data APPENDIX F Results of Individual Test Material ResidueAnalyses APPENDIX G Method Validation for FC-143 in Feed Method Validation for T-5180 In JtatSerum HWI 6329-100 232 233 2S4 275 295 620 621 622 629 630 644 645 651 231 HWI 6329-100 APPENDIX 0 IndividualAbsolute Organ Weight Data (g) Individual Organ-to-Body Weight Percentages IndividualOrgan-to-Brain Weight Ratios IndividualAnimal Pathology Data 232 HAZLETON LABORATORIES .AMERICA, INC. MADISON. WISCONSIN U S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA (0) S WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLTN)ES: SEX-ALL;ONOUP-ALL;WEEKS-ALL DEATH&I;suBSETeALL OR - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. fiESTIS-, TEI - NT. TESTIS. SO - ACCESS/SEX ORO SEX DOSE AN114AL TERMINAL GROUP NUMBER BODY WT 191 - - O-R - - - - -Li- - - - - L-U - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- 1 1 14 1 I m 1 m 1 m 1 m I m I m I m 1 I I m I mI C67451 C67457 C07461 C67404 CS?467 CB?461 C67476 C67477 C6?486 C67419 C67491 C67492 COYSOO C6?502 CS?503 447.6 3?S.? 409.0 425.9 420.S 398.0 419.9 420.1 397.0 382.7 381.1 451.9 401.7 409.0 418.8 2.2017 2.0693 2.1204 2.0479 2.1977 2.0281 2.07SI 1.9s79 2.1131 2.1238 1,9307 2.1730 2.0014 2.0260 2.0221 17.2631 15.3007 16.3219 17.2S?O 14.6161 14.9008 IS.SSOY 16.9781 18.3091 14.7941 14.4250 23.3031 15-5389 16.5645 15.SO99 1.7496 1.4270 1.5015 1.5181 1.6460 I.S$4s 1.4231 1..Glol 1.5415 .8718 1.4507 1.7054 1.5614 1.4831 1.6163 1.7809 1.5370 l.sris 1.0126 1.5841 1.7321 1.8496 1.4154 1.5448 1.8265 1.5006 1.7399 1.0913 1.66ss 1.6037 1.7540 1.5080 1.6495 l.asos 1.5651 I'lO$4 1.8101 1.3961 1.5492 1.7413 I.SI$2 1.8369 1.6935 1.0489 1,0466 2.6091 2.4754 2.1661 2.3400 2.1690 2.S$29 2.1944 2.3930 2.S$54 2.6769 2.7210 3.2461 2.4648 2.6034 2.6669 NU-MB-ER- -IN -GR-OU-P:- - - - - Is- - - - -Is- - - - - -Is- - - - -is- - - - - -15 - - - - -Is MEAN: STANDARDDEV: 411.1 21.7 2.0732 .0812 16.3408 2.1378 1.5138 .2006 1.0442 .12SO 1 0523 .1273 is- - - - - - - - - - 2.6801 .2257 -mm----22-----CC$6-7755-0190--------4369-40@.-l4--------21.-.09-9300-47-----1-7-.4-50-0-------1.-68-9-0 ------1-.7-28-7------1-.-70-32-------2.-80-4-1 -------------------------------- m 2 co7sil m 399.2 1.9731 14.2679 14.2649 1.6098 1.4519 1.6477 1.6229 1.6491 1.6199 3.1326 2,9169 2 C67514 m 2 celsis 440.2 396.0 2,1070 1.9549 16.1693 14.9406 1.5906 1.4492 1.8038 1.7679 2 6602 m 2 C67524 378.6 2.0306 15.3071 1.3105 1.5900 1.3102 1.5946 1.3303 2.2112 2.S370 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S,A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IG) 5 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL.WEEKS-ALL DEATH-I;SU13SET-ALL DR - BRAIN Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS, TEL - RT. TESTIS. SO - ACCESSISEX ORG SEX DOSE ANIMAL TERMINAL ----G-RO-U-P---N-UM-B-E-R---B-OD-Y--W-T --fg-l---------B-f-t-------------L-i-------------L--U--------------T-E-O--------------TE-L-------------S-o------------------------------------------ 2 C6752S m 2 C67529 t4 2 Co?530 2 C6753? m 2 C67S44 m 2 C67550 m 2 CGISSS m 2 C67558 m 2 C67SS9 434.7 404.5 422.6 359.6 387.7 387.9 422.1 433.7 488.4 1.8031 2.1694 2.1161 1.9303 2.0234 1.9827 2.0072 2.1439 2.0513 17.1185 14.7411 14.7171 12.1515 12.9862 14.1445 15.7892 17.5586 18.2277 1.5590 1.4026 I.SS$7 1.4473 1.4677 1.5451 I.S?76 L-.63S$ 1.0181 1.8906 1.7652 1.5715 1.6041 1.5412 I.SO24 1.4981 1.0699 1.5713 1.1599 1.7033 1.5950 1,6330 1.6271 1.4836 1.4914 1.6140 1.5797 2.7211 2.5456 2.6391 2,0156 2.S267 2.526S 2.7610 2.634S 3.5584 NU-MBE-R - -IN -GR-OU-P:- - - - -Is- - - - -is- - - - - -Is- - - - -Is- - - - - -is- - - - -I-s - - - - -Is- - - - - - - - - - - MEAN: STANDARD DEV: 413.9 34.S 2.027S .0842 IS.4495 1.7139 1.5312 .0989 1.4222 .1410 1.6168 .1243 2.0799 .3S92 ------------------------------------------------------W-----------------------w -------------------------------- m 3 C67562 433.1 2.0610 19.3861 1.4976 1.7701 1,7361 3.1278 m 3 C67565 390.4 1.9776 16.3602 1.4804 1.5178 1,5533 2 2069 m 3 C67566 m 3 CG?561 421.7 397.0 2.1092 1.9801 19.6938 17.9401 1.4756 1.3867 1.7084 I'si3i 1.7142 I.S234 2.6099 2 9710 m 3 C67569 * 3 C67574 389.8 396.1 2.0620 2.0008 16,0008 ie.isio 1.3330 1.4023 1.7269 1.8672 1.7674 1.8099 2 6631 3@4002 * 3 C67576 387.9 1.8902 17.4704 1.3139 1.6187 1.6220 3 1808 m 3 C67577 363.6 2.0559 16.0948 1.2149 1.5117 1.6657 2.3630 m 3 C67590 391.4 2.2534 17.2091 1.4866 I.SS2$ 1 S279 2.S?67 m 3 cols9i 379.3 1.9980 16.6659 1.4326 1.5782 l,s4ss 2.8492 m 3 C57592 341.6 2.1769 14.0101 1.2991 1.5136 1.5352 2.6531 m 3 c67s9s 414.9 2.0247 19.3969 1.5582 1.8551 1.2029 3.SS99 CONTINUED ON NEXT PAGE 44 HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ABSOLUTE OROAN WEIGHT 5 WEEK SACRIFICE DATA 10) ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GIOUP-ALL:WEEKS-ALL DEATH-I:SuBSET-ALL ON - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. F[STIS.- TEI - NT. TESTIS. SO - ACCESSISEX ORO SEX DOSE GROUP 3 m 3 m 3 AN114AL TERMINAL -- --- -- - -- -- -- -- - -- -- - -- --- -- -- - -- -- - --- NUMBER 13ODY WT (9) on Ll LU TEO TEI so ----------------------------------------------------------------------------------------------- cols9y C67599 C67601 310.0 407.5 480.6 2.0027 1.9288 2.1563 10.4221 20.7114 21.1925 1.4474 1.5289 1.?OSI 1.4949 1.7114 1.6701 l.ssol 1.6142 1.7340 2.6560 2.3845 3.3149 NU-MBE-R - -IN -GR-OU-P: - - - - -15- - - - -is- - - - - -is- - - - -i-s - - - - I-S - - - - -is- - - - - -15 - - - - - - - - - - - MEAN: 398.4 2.04S2 17.8942 1.4426 l.s4s4 I.OS20 2.8350 STANDARD DEV: 32.0 '0966 2.1294 .1122 .1241 loss .4016 ---------------------------------------------------------------------------------------------------------------- m 4 C67619 409.2 2.0962 26.3509 I.S62l I.S?38 l.s9l7 2.4021 m 4 CG?620 489.8 2.0154 2S.3547 1.4847 l.so6a 1.4304 3.1823 m 4 CB?625 m 4 C67627 371.2 396.2 2.1277 2.0441 20.1581 20.7728 1.5611 1.510S I.GS35 1.5123 l@674S 2.6636 m 4 C87630 4S7.0 2.0448 27-0511 I-SI23 1.0356 1.5975 1.6673 2.SIOI 2.9991 4 C67631 m 4 C67632 381.1 380.S 2.1312 1.9220 22.4129 18-2926 1.4438 1.3554 1,4191 l.s971 1.4oss 2.9913 m 4 C67633 4S3.7 2.1741 2S.6081 1.7S94 1.7336 I.Si$3 1.7207 2.39S3 3.2850 m 4 C6?636 385.3 2.0247 21.9217 I.SI17 1.5013 1.4497 2.4796 m 4 cole4o m 4 CO?648 397.0 392.0 1.9866 2.1307 23.9939 21.0322 1.3627 1.4050 1.5585 l.osl4 I.S$53 2.9113 m 4 c876s4 333.5 1.9196 21.1401 1.2542 1.5679 1.5671 1.5519 2.6953 2.7574 m 4 C6?659 416.0 2.1717 24.7761 1.6564 1.5126 1.4907 2.3142 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IG) 5 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL.GROUP-ALL.WEEKS-ALL DEATH-I;SUBSET-ALL on - a AIN Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - RLF. TESTIS. TEI - RT. TESTIS, SO - ACCESS/SEX ONG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT (9) - - e-ft- - - - -Li- - - - - L-U - - - - -TE-O - - - - -TEI- - - - -so- - - - - - - - - - --------------------------------------------------------------------------------------------------------------- m 4 C67663 m 4 C6766S 413.9 317.8 2.0214 2.0135 21.1079 27.2819 1.49S6 l.siso 1.6313 l.7s6s 1.6s23 2.3713 1.7867 2.4346 NU-MB-ER- -IN -GR-OUP-: - - - - -i-s - - - -15- - - - - -Is - - - - -Is- - - - - -is- -' - - MEAN: 403.3 2.0604 23.2303 1.4932 1.5874 STANDARD DEV: 35.5 .0759 2,8382 .1231 .0916 15- - - - - Is- - - 1.5104 lots 2.6973 .3136 --------------------------------------------------------------------------------------------------------------- m 5 C67675 401.9 2.0458 29.9182 1'.4415 1.5379 1.5878 2.7SIS m 5 C67611 372.9 2.0276 25.9118 1.4507 1.6202 1.0029 2.?151 m 5 C67682 373.0 2,14SS 26.9262 1.3795 1.5366 l.s994 2.2357 t4 5 C6768? m s C67689 313.1 392.4 2.2291 2.0018 22.9772 27.4930 1.4440 1.4362 1.5900 1.4394 l.70S4 1.4378 2.4616 m 5 C67690 365.2 1.9734 2S.5643 1.4011 l.s479 1.5556 2.2311 2@45SI m 5 C67692 m 5 C67093 m 5 CG?696 390.9 402.0 350.6 2.OS12 2.OS24 1.8946 26.0918 22.4973 24.8083 1.6213 1.432S 1.2528 1.7223 l.s36l 1.0194 1.7144 1 5114 1.5699 2.8217 3.1287 2.3663 m 5 C67091 m 5 C6?713 389 6 373.3 2.0041 2.2337 23 9691 2S.1610 1.3583 1.4625 l.es9i 1 6212 2.8625 m s C677is 387.8 1 9920 26.0457 1.4115 I-OSSY 1.7886 1.6381 l@1534 2.SISS 2.7715 m s C67717 361.0 2,0368 2S.1591 1.3601 1.6172 1 6543 2 2787 m 5 C6772S 346.6 1.9221 22.2038 l@3277 1.7531 1,1120 2.636S NU-MB-ER - -IN -GR-OU-P: - - - - -14- - - - -14- - - - - -14 - - - - -14- - - - - -14 - - - - -14- - - - - -14 - - - - - - - - - - - MEAN: 378.4 2@0437 25.4377 1.4140 I.S794 1.6260 2.6319 STANDARD DEV: 18.4 .0996 1.9900 .0828 .1704 .1013 .2S48 ---------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IG) S WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TA13LE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEAIH-I;SUBSET-ALL ON - BRAIN Ll LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS.- TEI - NT. TESTIS. SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT (9) - - O-N - - - - -Li- - - - - -LU - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - ---------------------------------------------------------------------------------------------------------------- N 0 CS?726 a C67727 m 6 C67733 N 6 C6773S m 6 C67741 m 6 C67742 m 6 C67745 m 6 C67751 m 6 C$7757 N 6 c677s9 6 C67761 m 6 CS?762 8 C67764 m 6 Col?69 m 6 C67770 371.4 308.9 384.1 383.7 393.S 386.0 396.0 317.0 403.1 402.3 391.9 388.8 392.0 402.7 362.5 2.0124 2.OIS4 1.1316 1,9243 1.9498 1.9377 2.0090 2.0547 2.1807 2.0779 2.0687 2.0710 1.9914 2.1204 2.0136 10.4989 IS.3142 16.7275 17.OSOI 15.6120 16.1264 15.1591 14.2522 16.2709 15.2773 15.4561 16.7sts 16.5557 11.3SO2 13.0996 1.4624 1.0498 1.5339 I.S4$2 1.5300 1.2100 l,.sls9 I.S?78 I.S378 1.4004 1.4140 1.5928 1.7763 1.7430 1.5011 1.2929 1.6110 1.7651 l.slo7 1.6557 1.6971 1.7713 1.4513 1.6187 1.5286 1.5977 1.6769 1.0393 1.6996 i.slos 1.3702 1.5435 1.7714 1.4701 1.7410 1.6902 1.7371 1.4922 1.6301 1.490S 1.5925 1.6002 1.6390 1.7401 1.4855 2.4971 2,1367 1.6099 2.2190 2.8005 2.1718 2.4131 2.4998 2.9101 1.977S 2-6961 2.6627 2.4532 2.2768 2.6226 NU-MB-ER - -IN -GR-OUP-: - - - - -Is- - - - -Is- - w - - -15 - - - - -Is- - - - - -IS - - - - -Is- - - - - -is- - - - - - - - - - - MEAN: 388.9 2.0172 15.8344 l.s376 1.8102 1.6001 2.4898 STANDARD DEV: 11.1 OIS7 1.1305 .1206 .1234 .1212 .3S?g ------------w --------------------------------------------------w------------------w---------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IG) I WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TA13LE INCLUDES: SEX-ALL;GROUP-ALL:WEEKS-ALL DEATH-2;SUBSET-ALL OR - BRAIN SLI LIVElt, LU - LUNG ORGAN ABBREVIATION: TEO - LF. tE TIS,- TEI - RT. TESTIS. SO ACCESS/SEX ORO !EX DOSE ANIMAL TERMINAL - -- -- -- -- -- - -- ---G-ROU-P---N-UM-B-E-R---BO-D-Y--W-T--(9-)------O-R ---------L-l--------L-U---------TE-O------------T-E-I------------s-o ------------------------------------- m I C6745S m I C674S$ 463.7 4SO.9 1.9797 2.0532 15.5440 l.esss 1.5477 I.S$14 2-yols I C67463 m 1 C67465 m 1 C67466 412.6 422.1 490.3 2.2372 2.1366 2.0832 16-06S17 16. 94S IS.4909 19.3221 1.5344 1.7812 1.4707 1.4311 1.6369 1.6%OS 1.8221 I.Sgoo 1,7104 1.7656 2.SS26 3.SSIO 2.S4$4 m 1 C87469 I C67472 494.0 sis.8 2.OISS 21.344S I.Sess 1.5484 1.6613 1.6171 1.7176 3.1184 3.0237 m I C67474 478.1 2.0210 2.0492 21.OSIO 17.5871 1.5394 l.sesi 1.97ss 1.7169 . i.gs5s 1.7102 3.3SSO m 1 CO?471 m I C67410 474.1 459.0 2.OSOS 2.1222 16-6694 16.4soo 1'.61043 1.6764 1.6441 2.3959 3.464S 1 CG?411 1 43S.1 2.OOSI IS.4702 1.3 40 l.s4l4 1.7231 1.5818 1.72S2 1.6941 2.1279 2.S2?6 Co?484 m I CG?494 480.7 4S2.1 2.1016 2.1980 18.2573 16.7S26 1.7544 1.64$S 1.7484 1.0606 1.7861 2.9813 m 1 CO?495 m 1 Co?498 485.5 soe.o 2,0187 lt.0970 1.$OS4 1.6692 1.6441 1.7320 3.0165 3.4893 2.1556 21.SOST 1.5832 I.STBS I.S?39 3.3224 NUMBER IN-GROUP: -- - 15 - - - -15 - - - - Is- - - - -is - - - - 15 - - - -is - - - - Is- - - - - - - - - - - MEAN: STANDARD DEV: 473.1 26.0 2.0923 .0696 17.7610 2.1244 I.S$29 .1077 1.6801 .1110 1.7031 IOS2 3.0673 .3617 -mm------22 -----Cc06-77-Ss-Ol62---------4-444-01-..1-4---------2-.-0-32-3-------1-5-.2-1-3-3--------I-.S-9-7-3--------1-.6-8-4-1 --------1.-0-0-19--------3-.-1-53-8------------ " 2 C67522 525.9 2.0451 2.0794 ls.lsoo 21.9998 1.6105 1,5873 1.5562 1.7694 1,4831 1.7859 3 0679 m 2 C67527 m 2 C67531 495.1 524.8 2.2223 2.0324 16-3032 19.1474 1.7404 l.7954 1.8030 3.3206 3.2105 m 2 C@7532 484.2 2.0003 17.3673 I.SS64 1.3694 1 5163 1.7019 1.5311 1.7102 2,7387 3.0757 CONTINUED ON NEXT PAGE co HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ABSOLUTE ORGAN WEIGHT 8 WEEK SACITIFICE DATA IG) --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH*2;SUIRSET-ALL ON - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. F[STIS,- TEI - NT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL ----GROUP NUMBER BODY WT (g) - - -BA - - - - -Li- - - - - L-U - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - m 2 -- --------------------------------------------------------------------------------------------------- c67s39 sio.? 2.0356 17.4985 1.60 9 1.7637 1.7937 2 C67540 m 2 C07542 2 C6754S 552.7 432.9 436.6 2.1032 1.0704 2.OSII 24.6735 14.974S 16.0053 1.18 3 1.57 2 1.6493 1.6001 1.6418 1.7024 3.3431 3.0235 2.2701 m 2 CG?546 M .2 Col$47 476.9 464.1 2.IIS7 16.19so I-SliSO I.S I 1.7131 1.6888 1.8184 1.6887 3.1652 2.7014 m 2 C67SS4 496.4 2.1074 2.1660 IS.3SY4 19.4011 1-:511 1. 1 I.S7$8 1.1070 1.6541 2.5313 2 C675SY m 2 ce7seo SOS.0 472.1 2.IOSS 17.0301 1.72 2 7846 1.0770 1.1211 3.S907 3.0050 2.0403 17.4734 1'.SI9S4 I..S902 1.5396 3.0806 NU-MB-ER- -IN -GR-OU-P: - - - - -is- - - - -Is- - - - - -15 - - - - -is- MEAN: - -- - -- -- -- -- -- -- - -- - --- - Is is Is 414.0 2.0739 17.6791 I-Bill 1.0866 1.6875 3.0990 STANDARD DEV: 36.6 .0649 2.7374 .1110 .0121 .10?6 .4021 -m-----3 ----CO-Y-ST-O-------4-4-2-.7--------2.-2-4-$-S -------w-------------------------------------------------------------------- m 3 ca7s?l 466.6 2,0477 18.0879 17.5462 1.4029 I-soge 1.8392 1.7192 3,9661 m 3 C6?580 m 3 C6?Sll 441.8 483.8 2.0828 19.3210 1.5312 1.774S 1.0539 1.6667 1 s6sl 3.2620 3.6804 m 3 C67512 m 4S?.3 1 933S 2.1754 l8.s77o 22.3152 1.7077 1.5590 1.7079 l.s470 1 6913 1.5773 3.4546 3 C67SI3 m 3 C67584 m 3 C675$S 67S86 453.8 497.1 4so.? 2.0247 2,0062 2.0252 18.1334 24.1724 19.3391 1.4036 1.80709 1.50641 1.4211 2.0352 1 SSS3 1 4119 1.9161 l@5890 3.1744 3 S?08 3.S310 3.0661 m 3C m 3 co7sel 413.7 493.0 2.0110 2.2704 IS.8206 19.S4?3 1.4 1.5:3 1 6005 L@SISS 3@5940 m 3 C67594 m 3 C67602 S23.8 504.0 2.0620 2.0971 21.29SS 1.6331 I.O7:S64 s 1.7142 1.7988 3 9945 3.9196 20.2097 1.6142 1.9214 1.8826 3.0233 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES . AMERICA. INC. MADISON. WISCONSIN U S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IG) I WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLWES: SEX-ALL;GIOUP-ALL:WEEKS-ALL DEATH-2;SUBSET-ALL OR BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO LF. TESTIS.- TEI - ItT. TESTIS, SO ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL - -- ----GROUP --N-U-MB-E-R---B-O-DY--W-T--1-9-1 ------OR--------------L-i---------L-U----------TE-O------------T-E-I------------so------------------------------- t4 3 m 3 CO?606 ce76l2 475.9 409.9 2.0932 2.0627 16.7144 1.2771 1.7080 i.isle 2.9217 m 3 CS?614 419.4 2.0062 19.8334 19.1089 1.45S0:29 I1.-6S2e0lSe I 695S 2.9461 1.6179 3.SS$e N UMBER IN-GROUP: 14EAN: - - -Is 470.9 -- -Is - - - 2.0152 Is- - - - -is - - - - -Is- - - - -15' - - - - -is- - - - - - - - - - - STA "DAND DEV: 28.6 .0881 19.419S 2.0972 1.5339 .1297 1.262664 . 96 1.6904 .1397 3.4448 .3649 -N-----4----C-S-?6-1-7-------4-21-.-8-------1-.-9-1-41-----------------:-------------------------------------------------------------- m 4 CG?629 SOS.2 2.1037 22.6410 27.1705 1.4212 I.SO19 I.Sele 2.4218 3.6044 m 4 CG?634 N 4 C67631 475.1 418.9 2.1873 24.6474 I.SOS4 1,8013 1.72942 1.195S 1.1230 2.IS63 3.0844 m 4 C67639 N 4 C67642 C6?644 479.0 S04.7 456.2 2.1911 2.OS44 2.ISOS 2.0121 26.5405 26.8281 30.2508 21.SOII 1.6116 1.4064 1.4 1.2S 2 1.46 7 1.9760 1.7243 1.4910 2.0156 1.7436 3.2016 3.2112 3.0769 m 4 m 4 C$7646 m 4 C$7647 471.6 2.0739 24.2SO2 1.7329I ISS2 1.7144 1.8384 1,7213 3.22S2 3.1011 m 4 C670SS 432.6 479.S 2.1070 2.1033 19.5653 23.8742 I.Sil 1.6199 1.61is 1.6080 1.6969 2.7296 m 4 C676se m 4 CS?657 405.4 469.9 2.0411 2.1091 21.4435 1.3888 1.900162 l.sois 1 9595 3.6047 2.0750 m 4 Co?eel 412.9 2.IS41 21.9$SS 17.9125 I.S92937 1.4 S 2.01 1.5281 1.9089 I.S247 3.2783 2.5619 CONTINUED ON NEXT PAGE coo HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IG) I WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GN OUP-ALL;WEEKS-ALL DEATH-2;SU13SET-ALL OR - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS,- TEI - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL ----G-RO-U-P---N-U-MB-E-R---B-OD-Y--W-T--(-91--------O--R--------------L-i--------------L-U--------------T-E--O -------------T-EI-------------s-o------------------------------------------ 4 C67662 m 4 C67667 431.7 494@5 2,0559 2.1607 20.07101 1.3732 1.5487 l.sll3 3.2091 2S. 940 1.4690 1.8?19 1.7338 3.5244 NUMBER IN-GROUP: MEAN: - - - -is 466.9 28.7 -is - - 2.1032 - is- - 24.0114 - -Is - 1.492012 - - Is- - 1.7163 - -15 - 1.7911 - - Is- - - - - - - - - - - .0650 3.5791 .1 7 .1649 3.1306 STANDARD DEV: .2401 .3184 ---------------------------------------------------------- m 5 C67680 m s C67699 430,9 2 0122 30.3922 n ------------------------------------------------------ 1.5506 1.5587 l.s2l6 2.8374 m s C67702 451.9 462 8 1.9812 1 9730 23.6452 25.0007 1.6440 1.7193 1.7874 1,6?SO 2.S243 5 CS?703 m 5 C67705 473 9 486.8 2.1139 2.0859 27.2361 1.6009 I.SS36 1.7440 I-SIS2 1.6116 3.1093 3.1259 m s C67707 N 5 497.S 2.1938 26.482S 30@2278 Il@-6SO35604 G7 S70 1. 721 l@S958 1 7930 2.sg2s C$7708 m 5 C67709 505.8 364,7 2.2070 2.1310 24.6949 23.2540 1 5957 1 6074 l@S917 3.1442 2.6153 m 5 C67710 m 5 C67711 m 5 C67718 453@l 448.5 479.7 2.0112 1 9929 2 1511 31.2147 23.6196 34,0653 1.3845 l@4683 .9224 1.5717 1.6261 1.8449 1.8353 I.SS77 1.6789 1.1308 2.3407 3.0033 2,4993 m 5 C67719 m s C61721 4?6.3 451.9 2.1768 30.9586 I-SO1?O 1.4320 1.6091 1 5203 1.6220 2.9666 2 9267 2.OISS 28.S4$4 1.4 sa l.87s6 1 8333 2.8522 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IG) 8 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEAT"-2; SUBSET-ALL OR - OR AIN Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS, TEI - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL ----GROUP NUMBER BODY WT (g) - - O-N - - - - -Li- - - - - L-U - - - - -TE-O - - - - T-EI- - - - -so- - - - - - - - - - -- --------------------------------------------------------------------------------------------------- 5 C07722 m 5 CS?124 429.8 472.1 1.0111 2.1162 25.0187 31.7972 1.5412 l.s794 1.8711 1.7S2? 1.6720 1.7111 2.9523 3.279S N@HiEft IN-GROUP: MEAN: STANDARD DEV: - - - -Is - - - -15 - - - - 15- - - - -Is - - - - IS- 459.0 2.0155 27.7557 1.5208 1.68$S 34.2 .0808 3.5077 .1904 .126S -15 - - - - IS- - - - - - - - - - 1.0533. 2.8513 .1070 .2775 ---------------------------------------------------------------------------------------------------------------- m 6 C67721 m 6 CG?729 m 6 C67731 438.1 460.1 44$.S 2.0153 2.0389 2.0231 16.4sis 19.8395 16.1915 1.3873 1.4861 1,6026 1.0152 1.7331 1.7209 1.6s$o 1.8101 1.7494 3.6721 3.10?1 3.5167 m a CS?734 m 6 C67737 416.5 460.5 2.0770 2.1044 15.569S 15.9088 1.4530 1.6SIO 1.5371 1.5238 3.0402 a C67739 m a C6?743 467.4 2.1329 16.0550 1.4oss 1.5674 1.4698 I.SI73 1,4410 3.1073 2.0103 6 C67750 438.9 448.0 2.2613 1.9796 17.6532 IS.5428 1.69si 1.3S20 1.62SI 1.4122 I.S2$0 13.4660EX 2.2069 N 6 C67752 m 6 C67753 m 6 C677se 490.0 475.7 445.6 2.1100 1.9936 2.1977 lg.l?19 21.3720 14.0527 I.SI44 I.SOS3 1.5914 1.7674 1.6930 1.5823 1.73S6 1.7542 l@6445 2.6175 2.6611 3.1131 2.9296 m 6 C67763 m 6 C67765 479.9 439.3 2.0385 2.1596 18.8615 16.4702 1.6338 1.7457 1.7633 1.7636 2.4933 I.ssis 1.5485 2.9217 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA, MADISON. WISCONSIN U.S.A. INC. INDIVIDUAL APPENDIX D ABSOLUTE ORGAN WEIGHT DATA (01 I WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATHo2;SUBSET-ALL ORGAN ABBREVIATION: OR - BRAIN S L LIVER, LU - LUNG TEO - LF. tE TiIs.- TEI - RT. TESTIS, SO - ACCESS/SEX ORO SIX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT 191 - - O-R - - - - -Li- - - - - L-U - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - ---------------------------------------------------------------------------------------------------------------- 14 a C67760 m 6 C67768 392.4 437.3 1.9598 2.0700 12.4009 17.7753 1.4526 1.7744 1.5927 1.8209 1.5625 1.7633 2.1564 2.7252 NU-M-BE-R -IN-GR-O-UP-: - - - -I-s - - - -is- - - - - -is- - - - -is- - - - - -Is - - - - -14- - - - - -15 - - - - - - - - - - - MEAN: 449.3 2.0862 16.9078 l.s6ls 1.6305 1.6335 2.8106 STANDARD DEV: 24.9 .0907 2.2232 .1317 .1177 .1111 .4391 -------------------------------------------------------1------------------------------------------------------- HAZLETON LABORATORIES AMEMICA. INC. MADISON, WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA (0) 14 WEEK SACRIFICE ----------------------------------------------------------------------------------------------------------------- TABLE INCLWES: SEX-ALL;GROUP-ALL WEEKS-ALL DEATH-T;S UBSET-AL@ OR - BRAIN Li LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF, FIESTIS-. TEI - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL ----GRO-UP----NU-M-B-ER---B-O-D-Y--WT--1-9-1--------O--N --------------L-i--------------L-U--------------T-E-O------------T--E-I-------------S-o----------------------------------- m 1 C67454 m 1 C67459 m 1 CG?460 m I C67462 m I CS?470 m 1 Co?4?3 m I C67475 m I C07482 m 1 C67415 m I CG?487 m I CG?418 m I C67499 m I C67501 m 1 COYS04 m 1 ce7sos SSO.7 495.8 S31.9 596.7 519.7 lo.s S667.4 511.8 5 S2935..46 21.2 6577.0 511.0 600.3 S48.8 2.1122 2.1392 2.4141 2.1057 2.0425 1.9927 2.3230 2.14$S 2.2lS4 2.3802 2.2904 2.20140 2.1 25 2.1334 2.1167 20.7989 15.7049 20.9863 18.3091 20.1604 23. 9641 20.4091 16.2988 21.0922 17.4732 22.4633 11.5020 11.9192 21.1053 19,6743 1.6404 l.s29 1.6768 1.410 1.518 I.S$42 1.7191 1,.GSI I.OISI l.s73S 1.7742 1.6464 1.00!7 1.84 48 1.511 .7305 I.IOS4 1.6212 1.7111 1.1097 1.7392 1.90is 1.9007 1.0466 1.71SO i.es4o I.SIS7 I.S330 1.1115 1.6138 1.7609 1.0211 1.6850 1.7438 1.7907 1.7830 1.9175 1.8097 1.9120 1.6766 1.623S 1.1021 1.5214 1.7134 1.6212 3.5431 2.9019 4.1448 4.2501 3.3156 3.524S 3.5652 3.1952 2.9746 4.2201 3.9901 3.1600 3.3014 2.6144 3.3370 ------- NU-M1-3EN- I-N -GR-OUP-: MEAN: - - - -Is- - - - -Is- - - - - -15 - - - - -Is- - - - - -Is- - - - -Is- - - - - -is- - - - - - - - - - - STANDARD DEV: 551.7 41.1 2.1914 .1113 19.7299 2.2290 I.OS43 .1403 1.7591 .1174 1.7572 .1126 3.S217 .4974 -m-----2 ----C-67-5-0-7------5-2-?-.-0-------3-.1-3-2-2-----16-.-9-7-92------1-.-51-7-S------------------------------------------------------------ m 2 Co?518 m 2 C:?Slg 467.2 SIS.4 2.0086 14.0143 1.3781 1 9512 1.6312 1.9094 1.7067 3@1032 3.1439 m 2 C667S21 m 2 557.6 2.1131 2.2002 16.7043 16.9153 1.6032 1.7320 1,16823 1.5 41 1.0267 1 6421 3,0481 C 7523 m 2 C 7526 613.6 2.1781 20.83S3 1.7937 1.8941 l@8579 3,4940 3.2076 513.2 2.1598 IS.4771 1.5033 1.6993 1.7274 2.9030 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ABSOLUTE ORGAN WEIGHT 14 WEEK SACRIFICE DATA ((I) --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GIIOUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL BR - OR AIN LI.- LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. fiEST . TEI - RT. TESTIS, SO - ACCESS/SEX OR(I SEX DOSE ANIMAL TERMINAL ----GR-O-UP----N-UM-B-E-R ---BO-D-Y--W-T --19-1--------O--R--------------L-i---------------LU--------------T-E-O-------------T-E-I-------------s-o---------------------------------------- m 2 COYS33 m 2 C67535 MISSING 2.013SEX ll.S414EX 1.49$OEX 1.6960EX 1.6749EX 3.5576Ex m 2 C67S36 549.3 570.3 2.1027 2.1616 17.0119 18.4336 1.6975 1.68 590 1.9135 1.8364 1,0137 1.8860 3.S420 3.0024 2 C67541 m 2 CG?549 562.1 609.S 2.1370 2.2603 16,4104 23.4192 1. 520 1.7373 1.5904 1.8229 1.5660 3.7192 m 2 csyssi m 2 C67552 m 2 C67SS3 583,4 610.2 Soo's 2@3262 @2.0215 2.1217 17.3152 23.8034 17.9913 I.SIS4 1.1710 1,7719 1.7933 2.0816 1.7765 1.8225 1.1257 2.5860 1.7760 3.9714 4.3740 3.$IOS 3.SO94 m 2 CG?556 532.3 2.2605 16.9166 1.4190 1.5240 1.5304 3.7276 NUMBER IN-GROUP: MEAN: STANDARD DEV: - - - -14 - sss.1 42.0 -14 - 2@2279 .2745 1-4-- - Is 035S 2.8120 -14- - - - - 1-4- - - - -14 - - - - 14- - - - - - - - - - - 1.6008 1.7329 1.8269 3.4797 .1816 .2279 .2499 .4208 ---------------------------------------------------------------------------------------------------------------- * 3 CS?563 * 3 C67564 * 3 C67571 3 631.7 S69.9 491.6 2.1397 2.1277 2.1255 26.7202 20,0061 17.3730 1.8608 11.S4629381 1.8004 1.8240 1,9603 1 7096 1.7241 1 9607 3.8610 3.9433 4.2992 m cal5ge * 3 C67598 511.5 S34.3 2 2403 2.0731 20,2379 22.4725 1.4079 1.6083 1,6654 1.6629 1 6369 4.5671 * 3 C67600 * 3 C6?603 595.1 2.2575 24-7003 1.7639 1.7801 1.6661 1 7600 2.8754 3.5697 * 3 C6?604 $09.4 S56.1 2.1709 2.1210 22.3011 19.9391 1.7242 1.7130 1.6844 l@9040 1,6867 1.8820 3.2306 m 3 C67605 * 3 C67607 S73.2 527.1 2.2442 2.0524 22.2101 1.6579 1.7716 1 7867 3 4395 3.2634 * 3 C67608 * 500.4 2.0934 17.8774 17.2484 .9333 1.5348 1.8299 1.8529 1.8216 1.7698 2 5374 3.2045 3 C67610 548.1 2.4094 18.4583 1.7268 1.7350 1.7383 4,0026 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IG) 14 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES! SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL OR - BRAIN Li - LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. TEST S. TEI - NT. TESTIS. SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WY 191 - - O-R - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -so- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- * 3 Co?ell *3 c6?6l3 529.2 583.2 2.1843 2.2002 18.1888 21.3304 1.420S 1.7412 1.6407 1.0482 1.7118 3.9513 * 3 Cs?ols 529.4 2.1700 16.8536 1.6116 1.8900 1.6651 1.1593 3.1010 3.2206 NU-MB-ER - -IN -GR-OUP-: - - - - -is- - - - -15- - - - - -is- - - - -is- - - - - -Is- - - - -Is- - - - - -Is - - MEAN: STANDARD DEV: S52.7 40.7 2.1740 .0896 20.4410 2.$GS7 I.S$03 .22SO 1.7767 .1017 1.?Slg .0772 3.5435 SS35 --------------------------------------------------:-@------------------------------------------------------------- * 4 C67616 * 4 coleis *4 C6?621 SIB.$ 596.6 607.0 2.1783 2.1651 2.2076 26.1955 22.0185 25.0527 1.6784 1.0356 1.7479 2.0033 1.7610 1.0589 1.9311 1.7267 1.630S 3.7400 3.9577 2,4959 4 C67023 m 4 c67624 4?0.7 530.7 2.1070 2.1104 17.9421 26.4596 1.5822 1.4814 1.0701 1.?078 1.6628 3.7735 m 4 C67635 544.9 2.OS9S 20.2680 1.5667 1.1801 1.7554 1.9577 3.3864 3.0409 m 4 C67641 m 4 C67643 545 4 4SS.6 1.93S4 2.0137 24,7112 17.6129 1.2912 1.7374 l.s79S 1.7110 1.6706 1 7418 2.S436 3@7702 m 4 C67645 m 4 C67650 621.6 556@3 2 2238 2.1319 24.2640 28.ZO76 l@6907 1.7301 1.9867 1.7603 2 0271 1.7022 3.1797 3,4488 m 4 C67652 506 3 2.0904 21.0718 1.5510 1.8529 1 8162 4 0830 m 4 C67653 m 4 C67651 592@l 444 1 2 0977 2.1339 28.IS$3 19.2486 1.8177 1.5343 1.8186 1.7072 1 1649 1.6782 3.3819 3.2944 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA (0) 14 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL BR - BRAINFESLI LIVER. LU - LUNG ORGAN A813REVIATION: TEO - LF. TIS.- TEI - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL -- -- -- - --- -- - --- - -- - -- -- - --- -- -- - -- -- -- - ----G-RO-U-P---N-UM-B-E-R---BO-D-Y--W-T--(9-)------1-3R---------L-l---------LU---------T-E-O --------T-EI--------s-o-------------------------------- m 4 C67668 m 4 C67669 414.9 S23.S 2.0351 2.OGIS 14.2?SO 24.OS67 I.S2SI 1.5117 1.6947 1.6942 2.9082 - -- - - - -1 - - - 1.712S 1.77$S 3.6146, NUMBER ,.IW GROUP: 5 15 is- '*- - -Is - - - - 15- -15 - - - - Is MEAN: STANDARD DEV: S38.2 2.1081 22.?367 1.6101 1.7771 I.IISO - -- -- - -- -3.3760 56.1 .0724 4.2128 .1316 .1216 .1197 .4782 -------w -----------------------: ---------------------------------------------w---------------------------- m 5 C67671 5 C67672 550.6 459.5 2.2283 2.1400 21.9610 20.0820 1.7189 1.4046 1.9706 1.9721 3.5D$7 m s CGYS?3 m 5 C67677 501.8 2.3368 23.3503 I.SO36 1.6796 1.7336 1.6773 1.70$S 3.3349 3.3136 m 5 CG?6?9 S32.1 43?.4 2.1273 2.0367 23.0049 28.2020 1.43?6 1.5392 1.622S 1.8515 1.6322 l@7670 2.6019 m s Col$$G m s C67618 MISSING 412.5 2.1931EX 2S.9412EX 2.2203 1.62S3EX 1.8143EX 1.126$EX 3.OS$4 3.1612EX m 5 C67691 405.9 1.9935 25.7S41 19-6478 1.4103 1.2989 1.5410 I.SO29 1.6223 1.4089 2.3141 2.8857 m 5 C67895 m 5 C67697 m 5 C67700 N5 3$S.9 481.7 558.6 2.1189 2.1143 2.0545 21.7197 30.4173 34.0432 1.3370 1.4679 1.7753 1.8195 I.IIS9 2.0474 1.7250 1.8220 1.9584 3.0418 4.1308 4.1528 C67712 m 5 C6?714 583.8 41S.5 2.2038 2.IS74 33.3805 28.6577 1.8828 1.4941 1.0780 1.6633 1.8628 4.1255 1.7522 3.2741 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA IGI 14 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEXaALL;GRDUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL OR - BRAIN Ll LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS.- TEL - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL ONOUP NUMBER BODV WT 191 - - O-R - - - - -Li- - - - - -LU - - - - -TE-O - - - - -TEL- - - - -So- - - - - - - - - - --------------------------------------------------------------------------------------------------------------- N 5 COYYIO 464.7 2.2060 21.3462 l.sole 1.8894 1.6342 2.1111 5 C67720 658.1 1.9403 36.3471 I.SO44 1.7913 1.7716 4.40SO NU-MB-ER - -IN -GR-OU-P: - - - - -14- - - - MEAN: 419.1 STANDARD DEV: 79.0 ----------------------m--------- m 6 C67730 6 CS?732 m a CS?736 m 6 C67730 m 6 CO?740 m 6 C67744 m 6 C67746 m 6 C67747 m 6 C67741 m 6 C67749 m 6 CB?754 m 6 C6775S m 6 C677SS 490.1 S32.2 516.S 523.1 466@7 503.7 474.3 S36.4 503.4 495.6 S46,3 473.7 490.0 -14- - - - - -14 - - - - -14- - - - - -14 - - - - -14- - - - - -14 - - - - - - 2.1342 26.7846 1.5123 1.7576 1.7373 3.3047 .1044 S.4675 .1401 IS47 .1460 .0309 -----m ---------m---------m -----M ---------- 2.0387 2.2336 2.1141 2.1391 2.1000 2.2132 2.1136 2.1360 2.1320 2.1303 2.1798 2,1551 2.0271 16.3SOO IS.6333 15.8738 17.0909 15.2555 16,5820 14.3227 20.1881 IS.9957 16.2SOO 18.1830 13.9650 16.321S 't.5931 I.S?Ss 1.4705 1.6276 1.3691 1.5761 1.7248 1.4971 1.62S2 1.5980 1.7299 1.7195 1.5033 1.6753 2.OS43 1.7049 1.950S 1.6609 1.6972 1.0021 1.9121 1.6997 1.6322 1,7984 1.7220 1.5453 m -----------M--------------- 1.6759 1.0868 3.3606 2.2448 1.7359 1.7333 1.6399 1.7301 3.2384 3.2127 3.096S 4.6774 1.5768 I.S406 1.7396 3,4281 3.5361 3,7221 1.5716 2.8874 1.8016 4,4033 1.7385 2.2831 I.SS3S 3.0065 CONTINUED ON NEXT PAGE 60 co HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S,A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA 101 14 WEEK SACRIFICE ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL GROUP-ALL;WEEKS-ALL DEAT"-T';S USSET-ALL OR - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. FIESTIS-. TEI - Nt. TESTIS. SO - ACCESS/SEX ORO SEX DOSE ANT14AL TERMINAL GROUwP---N-UM-B-E-R---B-Ow-D-Y--W-T--19-1---------O-N--------------L-i--------------L-U--------------T-E-O------------ -TEI- - - - -So- - - - - - - - - - - s C677so m 6 CO?767 496.1 2.1114 15.84S2 I.SOSO4 w ----------------------w ----------------- 1.5840 1.5650 S03.0 2.2220 16.1837 1.536 1.69so 1.7503 2.76SY 3.0260 N u-mi-3c-p I-N -GMR-EOAU-NP::- - - - -S0I3s-.5- - - -152-.1-30-4 Is 15 Is Is Is - - - - - - - - STANDARD DEV: 23.7 .0594 16.3033 1.6241 I.SI75 .1018 1.7117 .1311 1.6903 .1204 3.2597 .663S ---------------------------------------------------------------------------------------------------------------- HAZLETON MADISON, LABORATORIES . AMERICA. WISCONSIN U S,A. INC. INDIVIDUAL APPENDIX D ABSOLUTE ORGAN WEIGHT DATA (G) 22 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GIIOUP*ALL;WEEKS-ALL DEATH-U;SUBSET-ALL ORGAN ABBREVIATION: 13R - BRAIN Ll LIVER. LU - LUNG TEO - LF. TESTIS.- TEL - RT. TESTIS. SO - ACCESSISEX ORO SEX DOSE ANIMAL TERMINAL - -- -- -- --- I GROUP NUMBER BODY WT (9) OR Ll - - L-U - - - - -TE-O - - - - -TEL- - - - -so- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- m I C87452 m 1 C67453 S$4.9 666.3 2.IISS 2.1303 18.03SI I.SISO 1.0430 1.7469 4.3278 N I C67450 564.9 2.1450 23.6464 17.3831 l.lss3 1.59232 1.0776 1.4105 2.0904 1.4tol 3.5066 3.5651 m I C67471 1 C67479 m I C67483 653.1 489.7 SIS.4 2.2174 2.0754 2.2214 23.0661 14.35so 19.795S 1.6 03 1.4153 1.5677 1.7540 1.5307 1.7633 1,7921 l.sloi 1.7407 3.1614 2.4799 3.75$S m I C67490 m I C67493 m I C67496 610.6 510.4 507.2 .2.0230 2.0441 2.0397 19.8409 15.5493 15.7533 1.6371 L-5130 1.50719 1.7820 1.7096 1.7336 1.8633 1.0119 1.1395 3.1663 2.9256 I C67497 597.7 2.2377 19.OOSS 1.5 23 1.6288 1.664S 3.2306 3.9372 NU-MBE-R - -IN -GR-OUP-: MEAN: - - - -10- 571.1 - - -10- - 2.1328 - - -10 - - lt@6432 - -10- - - - - -10 - - - -10 - - - - -10 - - - - - - - - - - STANDARD DEV: 61.6 .0822 3.0867 1.5981 .1050 1.0934 .1536 1.7544 .1811 3.5540 .5410 ---------------------------------------------------------------------------------------------------------------- m 2 CS?SOI m 2 C07513 m 2 C67516 S63.4 680.3 671.8 2.1800 2.29S7 2.2907 11.0139 21.4301 19.8095 1.7534 1.90SO0 I.OS2 1.546S I.OS22 1.1136 1.6334 1.7142 1.8377 3.8153 3.9260 2 colsi? m 2 C67520 all.$ SSI.1 2.2742 2.2930 18.9402 15.6840 1.6369 1.6511 2.0113 1.7258 2.1674 1.7081 3.2904 4.2763 4.2444 2 CB?521 m 2 COYS34 m 567.3 610@9 2.2437 2.1679 17.2706 17.1901 l.sato 1.7502 1.7632 1.8921 1 7366 1.1614 3.6242 3.5062 2 C67s38 692.6 2.2904 20.1106 1.9530 1.8505 1.7197 3.8643 CONTINUED ON NEXT PAGE C)- HAZLETON MADISON, LABORATORIES AMERICA, WISCONSIN U.S.A. INC. INDIVIDUAL APPENDIX D ABSOLUTE ORGAN WEIGHT DATA IG) 22 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-U;SUBSET-ALL ORGAN ABBREVIATION: OR - BRAIN Ll LIVER, LU - LUNG TEO - LF. TESTIS.- TEL - RT. TESTIS, SO - ACCESS/SEX ORG SEX DOSE AN114AL TERMINAL GROUP NUMBER BODY WT 19) - - -13R - - - - -Li- - - - - -LU - - - - -TE-O - - - - -TEL- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- m 2 C67SA3 m 2 C67548 S46.2 560.7 2.2020 2.2262 IS.9S93 17.9184 1.7226 1.5154 1.4962 1.7379 1.5067 1.7006 2.3864 3.5671 NU-MBE-R - -IN -GR-OUP-: - - - - -10- - - - -10- - - - - -10 - - - -to - - - - -10 - - - -10 - - - - -10 - - - - - - - - - - MEAN: STANDARD DEV: 607.1 St.1 2.2465 .0496 18.4373 1.7410 1.741S .1541 1.7559 .1607 1.7592 .1739 3.6582 .5408 ---------------------------------------------------------------------------------------------------------------- 3 C67561 m 3 C07S07 3 CSYS72 N 3 C67573 m 3 C67575 m 3 calSY9 3 C67ses m 3 colsig m 3 C67593 m 3 C67609 NUMBER IN GROUP: MEAN: STANDARD DEV: 612.8 2.2294 11.9096 1'.5853 1.3128 1.6840 4.2129 640.3 553.3 S26.7 2.3313 2.2251 2.1454 11.9256 18.4045 14.1221 1.66577 1.7 is 1.5733 1.7147 1.0419 1.7071 1.1104 1 5651 1.1022 3.2180 3.3023 3 6631 729.2 2.2754 22.2539 1.7949 2.0338 2 0447 3.4610 S$7.0 571.7 2.1446 2.IIS7 18.3910 17.7967 1.6293 1 5797 I.YS70 1.7759 3.1273 830.8 2.1870 21.7957 2.1373 1.5047 2.023S 1,SG63 2.1192 3.SG72 3 6165 709.5 730.9 2.0594 2.2589 20.5211 IS.OIS2 l.ssoo 1.5208 1.8691 1 8484 3.7032 w 1.7566 1.8681 3.2434 10- - - - -10 - - - - to-:- - - -10- - - - - -10 - - - - -10- - - - - -to - - - w 629.2 2@2042 18.9203 1.6800 1.7391 1.7991 3.5191 73.4 .0774 2.2961 .1827 .2194 .1134 .2931 - -- - --- ---------------------------------------------------------------------------------------------------------------- m 4 C67622 m 4 C67626 563.6 2.1055 21.3009 I.S222 1.8721 2.0008 3 87S9 m 4 C67628 560.2 666.5 2.0641 2.2129 18 6121 21.4594 1.634S 1.7619 1.1775 1.8651 1.1955 1.8660 4 2949 4.4556 CONTINUED ON NEXT PAGE 04 HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ABSOLUTE ORGAN WEIGHT DATA (01 22 WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-U;SUBSET-ALL OR - 04AIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS,- TEI - PT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL ----G-R-OU-P---N-U-M-BE-R---B-O-DY--W-T--(-g-)--------O-R--------------L-i---------------L-U --------------TE--O------------T--E-I-------------So----------------------------------------- m 4 C67637 S29.9 2.1437 22.6290 1.6894 I.S?30 1.6060 3.3451 m 4 c67649 601.S 2.1373 17.4747 I.SIS3 1.2237 1.9322 4.0803 m 4 C67651 614.9 2.1395 20.760S 1.6285 1.8001 1.7725 3.2S37 m 4 C67660 ssi.1 2.3756 20,6451 1.72S? 1.7347 1.7106 3.8045 m 4 CGI$64 694.3 2.1989 20.9493 2.0241 1.6828 1.8949 4.3942 N 4 C$7666 609.1 2.2312 19.4S7S 1.6434 1.4773 1.4605 4.0238 m 4 C87670 620.S 2.2129 24.0443 1.7924 1.9480 1.9042 4.0490 NU-M-BE-RI-N-G-RO-UP-:- - - -1-0 - - - -10- - - - - -10 - - - - - 7-10 - - - -10 - - - -10 - - - - -10 - - - - - - - - - MEAN: 601.1 2.1122 20.7334 1.6937 1.705S 1.7913 3.9577 STANDARD DEV: 51.2 .0863 1.1917 .1476 .2244 .1655 .40SO ---------------------------------------------------------------------------------------------------------------- m s C67074 m 5 CG?076 m 5 C67670 N s C6?613 m 5 C67614 m s CO?094 m s C67701 m s C67704 OSS.4 399.4 SS2.S 593.0 490.9 59S.7 541.8 S97.4 2.2178 2.2129 2.0649 2.14S7 2.0711 2.2788 2.0228 2.OOS3 21.6330 IS.9177 19.4957 17.4588 14.1733 20.0263 19.0210 19.9$SO 1.6911 1.4120 1.4101 1.5412 1.4S41 1.7747 1.0734 l.s285 1,9654 1.7404 1.72?S I.SS34 1.6sso 1.8411 1.73S3 1.6107 1.0083 1.6794 1.7204 I.S?33 1.7138 1.7210 1.7206 1.0761 3.9142 4.SS?4 3.6056 3.639t 2.4774 4.1212 4.8046 3.02SI CONTINUED ON NEXT PAGE t%3 HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ABSOLUTE ORGAN WEIGHT DATA (0) 22 WEEK SACRIFICE ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL; GROUP-ALL;WEEKS-ALL DEATH-U.SUBSET-ALL OR - BRAIN Ll LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. fiESTIS-. TEL - RT. TESTIS. SO - ACCESS/SEX ORO SEX DO$[ ANIMAL TERMINAL ----G-RO-U-P---NU-M-B-E-R --B-O-D-Y--W-T --(2-)--------O--N--------------L-i--------------L-U--------------T-E-O-------------T-E-L-------------S-o------------------------------------------ 5 C67706 m 5 CS?723 572.9 561.3 2.1820 2.1428 21.SO33 10.9088 1.5610 I.SGS9 1.7092 2.0423 1.6788 1.9492 3.429S 3.2844 N-UM-BE-RI-N-GR-O-UP-: - - - -10- - - - -10- - - - - -10 - - - - -t-o - - - - -10 - - - - -10- - - - - -10 - - - 14EAN: STANDARD DEV: SSO.0 69.1 2.134S .0909 11.6190 2.446S l.s9l9 .1130 I.YSOO IS09 1.735S .1118 3.6939 .6923 ---------------------------------------------------------------------------------------------------------------- "AZLETON LABORATORIES . AMERICA. INC. MADISON, WISCONSIN U S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIG"T 5 WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;OROUP-ALL;WEEKS-ALL DEATHol;SUBSETBALL GR - BRAIN SLI LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF, tE TIS.- TEI - RT. TESTIS. SO - ACCIESS/SEX ORO SIX DOSE ANIMAL TERMINAL --- -- -- - -- -- -- -- - -- - -- -- --- -- -- - -- - --- - - ----GRO-U-P ---NU-M-B-ER---B-O-D-Y--WT---(9-1------a-n---------L-l--------L-U---------T-EO---------TE-I--------s-o------------------------------- I CS?451 m I C67457 447.6 375.7 .4919 .5sol 3.8570 4.0747 .3909 .3798 .3979 .3919 .5829 m 1 C67461 409.0 .5184 3.9907 .3 .4001 .31'59 .4014 .4033 .7121 .7001 m 1 C67404 1 C67467 m 1 C67468 425.9 420.5 398.0 .4101 .5226 SO97 4.0519 3.4025 3.7430 .3566741 .3914 .3981 .3716 .3767 .43S2 .3875 .3722 .4s44 .5494 .6023 .6490 m 1 C67476 m 1 C67477 419.9 420.1 .4942 .4661 3.7770 4.0414 .33 9 ...3S86, .4405 .3369 .4311 .3325 .6893 m 1 C57480 m 1 CG?489 397.0 382.7 .5324 .5550 4.1081 .3883 .3891 .3902 s69s .6512 1 C67491 388.1 .4995 3.8659 3.7168 .2296 .3738 .4773 .3107 .4561 .3912 .699S .7029 I C67492 m I COTSOO 4SI.9 401.7 .4809 .4982 s.issy 3.8603 .3774 .3187 .3$SO .4065 .7182 m I CS?502 m I C67SO3 409 0 418.8 .4955 .4828 4.0500 3.7249 .3626 .3859 .4210 .4072 .3129 .4216 .4032 .3932 .6136 .6365 .0368 NU-MBE-R - -IN -OR-OUP-: - - - - -i-s - - MEAN: 411.1 STANDARD DEV: 21.7 -Is- - - - - -Is- - - - -15- - - - - -15 - - - .5053 3.9610 .3076 .4007 .0260 .3714 .0413 .0332 -15 - - - - is- - - - - - - - - - - .402S .0309 .6530 .0545 -m----2-----CO-Y-S0-9-------4-64-.-1---------.4-1-54------3-.-7-54-3-------.-30-3-4------.-3-7-1-9 ------------------------------------------------- 2 coisto m 2 C67511 m 2 CG?514 390.4 399 2 440.2 .5355 .4944 .4786 3.6s47 3.5734 3 6732 .4123 .3655 .3613 .4221 .4065 .4098 .4224 .40SI .4016 .6033 .1024 .7307 .6061 m 2 C67515 m 2 C67524 396.0 376.6 .4937 S392 3.7729 4.1973 .37 1 .356ot .4017 .3479 .4027 .3S32 .565731?4 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 5 WEEK SACRIFICE PERCENTAGES --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEXOALL;OROUP-ALL;WEEKS-ALL DEATN-I:SU13SET-ALL ORGAN ABBREVIATION: OR BRAIN Ll LIVER, LU - LUNG TEO LF. TESTIS.- TEI - NT. TESTIS, SO - ACCESS/SEX ORG SIX DOSE ANIMAL TERMINAL GROUP ----- NUMBER ------ BODY WT ---- -- (9) --- - - -OR - - - - -Li- -* - - - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - --- --- m 2 C67525 m 2 C67529 m 2 CS?530 434.7 404.S 422.6 .435S .5363 .5007 4.0990 3.6443 3.4827 .3586 .3467 .3688 .4349 .4364 .3138 .4279 .4211 .3774 .6260 .6293 .6247 m 2 C67537 m 2 CeIS44 359.6 387.7 .5361 S219 3-SYSS 3.349S .402S .3786 .4461 .4S41 .5605 m 2 ce7sso 387.9 .5129 3.6464 .3983 .3975 .3873 .4197 .382S .6517 .6Sl3 N 2 C67555 2 CGISSI 422.1 433.7 .47SS .4943 3.7406 4.0486 .3738 .3772 .3549 .3$SO .3S33 .6541 N 2 ceiSS9 411.4 .4200 3.7321 '.3313 .3232 .3721 .3234 .6074 .?286 NU-MB-ER - -IN -GR-OUP-: - - - - -is- - - - -is- - - - - -15 - - - - -is- - - - - -IS - - - - -is- - - - - -15 - - - - - - - - - - 14EAN: STANDARD DEV: 413.9 34.S .4927 .0416 3.7297 .2293 .3710 .0216 .3933 .0350 .3922 .0350 .6472 OOSY --------------------------------------------------------------------------------------------------------------- m 3 C67562 m 3 C6756S m 3 c67s66 433.1 390 4 421.7 .4751 .5066 SO02 4.4689 4.1906 4,6701 .34S2 .3807 .3499 .4080 .3888 .40si .4002 .3979 .4065 .7210 SSS3 .6119 m 3 C67568 m 3 C67569 397.0 389.8 .4988 .5290 4.5189 4.1049 .3493 .3420 .3813 .3837 .7484 m 3 CB?574 396.8 .5042 4 0703 .3534 .4430 .4706 .4534 .4ssl .6134 .8569 m 3 Co?576 m 3 C67577 m 3 Co?590 387.9 363.6 391@4 .4873 .5654 .5757 4.5038 4.4265 4.3961 .3307 .3534 .3798 .4173 .4350 .3967 .4181 .4581 .3904 @8221 .6499 .6583 m 3 C6752t m 3 C67592 379.3 341.8 .5260 .6369 4 3939 4 0989 .3777 .3803 .4161 .4075 .7512 m 3 C67595 414.9 .4880 4.6751 .3756 .4428 .4471 .4492 .4345 .7762 .8sto CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC, MADISON. WISCONSIN U.S.A, INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 5 WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-I;SUBSET-ALL OR - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS.- TEI - RT. TESTIS, SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL ----G-RO-U-P---N-U-MB-E-R---B-O-DY--W-T--(-9-)---------OR--------------L-i---------------L-U --------------TE--O------------T--E-I-------------so------------------------------------------ m 3 C67597 m 3 ce7soo 380.0 407.5 .5270 .4733 5.10113 S. 843 .3809 .3934 .4007 .6919 m 3 C67601 410.0 .4487 4.5552 .3?52 .3548 .4200 .4133 .3610 S$52 .6197 NUMBER IN-GROUP: MEAN: STANDARD DEV: -is - - - -Is - - - - 15- - - - -Is - - - - 15- - - - -Is - - - - Is- - - - - - - - - - - 391.4 32.0 .5162 .04?4 4.4846 .3152 .3025 0162 .4142 .0310 .4160 .0290 .7122 .0910 ----------------------------------------------------------------------------------------------------------------- 4 C67619 m 4 C67620 409.2 469.8 .5123 .4290 6.4396 5.3969 .3117 .3848 .3907 Selo m 4 C:762S. m 4 C 7627 N 4 C67630 3?1.2 396.2 4S7.0 .5732 SI59 .44?4 5.4305 5.2430 5.9193 .3100 .4207 .3112 .3309 .3207 .44S4 .3117 .3S?9 .3064 .4SIt .4032 .0774 .7176 .6487 m 4 C67631 m 4 CG?632 311.1 386.S .5592 S.Olli .31 9 .3720 .3641 .3688 .6563 .7867 m 4 C67833 m 4 C67636 453.7 335.3 .4973 .4792 .5255 4.7329 5.0443 5.6895 6.0431 .3So,l .3878 .3942 .4132 .3121 .3896 .3928 .3793 .3763 .6197 .7240 .6436 m 4 C67640 m 4 C67648 m 4 397.0 392.0 .5004 s4sl 5.5114 .3432 .3514 .3926 .4213 .3993 .3998 .7333 .6876 C@7654 N 4 csloso 333.5 416.0 .5966 .5237 6.3319 5.9sss .37 .39:2' 4701 .4653 .8268 .3636 .3583 .5563 CONTINUED ON NEXT PAGE 00 HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT S WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-I:SUBSET@ALL OR - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS.- TEI - NT. TESTIS, SO - ACCESS/SEX ONG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY Wt fg) - - I-RR - - - - -Li- - - - - L-U - - - - -TE-D - - - - T-EI- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- m 4 C67663 m 4 CG?665 413.9 387.8 .4184 5.0991 .3613 .3941 .3992 S?29 SI92 7.1898 .3907 .4S.29 .4607 .6278 HU-14-BER- -IN -GR-OU-P: - - - - -I-s - - - -i-s - - - - -Is - - - - -I-s - - - - -Is- - - - -is- - - - - -Is- - - - - - - - - - - MEAN: 403.3 S142 5.7682 .3713 .3962 .3944 .6710 STANDARD DEV: 3S.5 .0444 .0020 .0278 .0291 .0414 .0761 --------------------------------------------------------------------------------------------------------------- 5 C676?S * 5 C67681 408.9 372.9 SO03 .5437 7.3161 6.0417 .3S25 .3890 .3761 .4386 .3113 .6729 * s C67682 373 0 .5752 7.2188 .36 1 .4120 .4291 .4281 .7281 .7602 * 5 C67687 s C67619 m 5 CG?690 383.1 392.4 365.2 .5819 slot .5404 5.9977 7.0064 7.0001 .37:9 .3060 .3837 .4166 .3691 .4231 .44S2 .3664 .4260 .642S s7os .6723 m s COT692 m 5 Co?693 390.9 402.0 S247 sios 6.6748 5.5963 .4141 .3SO3 .4406 .3821 .4316 .7214 m s C07696 3SO.6 .5404 7.0760 .3s73 .3107 .3760 .4470 .7783 .0749 s C$7698 319.6 S144 S.IS22 .3416 .4260 .4161 .7347 m 5 COI?13 373.3 .5914 6.9277 .3911 .4443 .4381 .6739 m 5 C077is m 5 C67717 317.8 361.0 .5139 SS42 0.7163 6.969S .3658 .3792 .4612 .4480 .4779 .7105 m s C6772S 346.6 .5548 6.6011 .3131 .50SI .4S$3 .4939 .6312 .7607 N@MiEn IN-GROUP: MEAN: STANDARD DEV: -14 - - - -14 - - - - 14- - - - -14 - - - - 14- - - - -14 - - - - 14- - - - - - - - - - - 378.4 S409 6.7292 .3739 .4179 .4308 .69se 18.4 .030S .4938 .0181 .0475 .0359 OS79 ----------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 5 WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GMOUP-ALL;WEEKS-ALL DEATH-I;SUBSETMALL 64 - BRAIN Ll LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. fiESTIS-, TEI - RT. TESTIS. SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY Wt 19) - - -ON- - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- 6 C67726 m 6 CG?727 371.4 388.9 .5411 SI$2 4.4424 3.9371 .3938 .4242 .3481 .4142 .3705 .3969 .6723 .7294 m 6 C67733 384.1 .4769 4.3550 .3993 .4s95 .4612 .4191 m 6 C6773S m 6 C67741 6 C67742 383.7 393.5 386.0 .5015 .495S .5020 4.4453 3.9675 4 1778 .403S .3118 .3316 .3937 .4208 .4397 .3831 .4426 .4379 S7$3 .7117 .7440 N 6 CB?745 6 CO?751 396.0 387.0 .5073 .5309 3.8212 3.6827 .3121 ...4077 .4491 .3?SO .4318 .6094 m 6 C67757 403.1 S410 4.0304 .3815 .4016 .3856 .4046 .6459 .7221 m 6 C677sg m 6 C67761 402.3 391.9 .5165 .5270 3,7975 3.9439 .3481 .3608 .3949 .3705 .4915 m 6 C6?762 381.2 .5327 4.3103 .4097 .4077 .4313 .4064 .4116 .6810 .6849 m 6 C6?764 m 6 Col?69 392.0 402.? .5010 .526S 4.2234 4.30$S .4531 .4328 .4182 .4221 .4181 .62se m 6 C67770 302.5 .5555 3.6137 .4141 .4332 .4321 .4091 .5654 .7235 HU-M1-3EN- -IN -GR-OU-P: MEAN: - - - -I-s 388.9 - - -Is- - - .5111 - -is- - 4@0714 - -15- - .39SS - - -Is- - .4139 - -Is- - - - - -is - - - - - - - - - - - STANDARD DEV: 11.1 .0207 ..2734 .0319 .0288 .4113 .0214 .6401 .0943 ------------- -------------------------------------------------------------------------------------------- tn co HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT I WEEK SACRIFICE PERCENTAGES ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL ORGAN A13OREVIATION: OTREO -_ORLAF. SLIS- LIVER. LU - LUNG '"fE TI TEI RT. TESTIS. SO - ACCESS/SEx olto SEX DOSE ANIMAL TERMINAL -- - GROUP N 1 NUMBER BODY WT (9) 8 It - -------------------- - -----L-i--------------L-U----------m --T--E-O--------T-E-I--- SO m C6745S 463.7 .:2S$9 3.3522 .3S?o .3338 -----M --------------------- Co?450 14 1 C07463 4so.9 482.6 .s 4 .4636 3-SO321 3.4 16 .3403 .3630 .342S .3S26 SOSS Soot 1 CB?405 422.1 SO62 3.7173 .3064 .3484 .3439 .3544 .7373 m 1 Co?466 N 1 C67469 490.3 494.0 .4249 .4201 3.9410 .2019 .4317 .31so .4113 .3300 .6037 .0413 m 1 C$7472 516.8 .3912 4.3207 4.06147 .3236 .2979 .3303 .3477 .6121 m I C67474 1 C87471 470.1 474.1 .4286 .432S 3. YIS .. ..3274 .3823 .3591 .3842 .3723 .0492 .7103 1 C67410 1 4s9.0 .4624 3.SISO 3.5139 384 .30is .3S3@ .3754 .3469 .31SO .7308 C67411 m 1 C67414 43S.1 486.7 .4$IS .4332 3.SS69 3.7SI2 .3S43 .36S2 .3895 .6161 Slog CG?494 C6749S 4S2.1 .4862 3.7055 .360S .3646 .3592 .3673 .3670 .3837 .8140 .6672 m 1 C67498 4$S.5 soe.o .4158 .4260 3.72?S 4.2SO7 .343 .31209 3430 -.3116 .3SOY .3110 .7187 .$See NUmMB-ER- -IN -GR-OUP: - - - -is- - - - -Is- - - - - Is - - - - -Is 14EAR: STANDADR DEV: 473.1 26.0 .4436 .0311 3.7451 zlso .33S2 .0249 is- - - - -is- - - - - -is- - - - - - - - - - - - .3561 .3609 .0471 .0291 .02SO OSSY 2 C67500 m 2 C67S12 m 2 C:7S22 m 2 C 7527 m 2 C:7S31 m 2 C 753 CONTINUED ON NEXT 441@4 440.1 s25,9 495.1 524.8 484.2 PAGE ----- .4604 .4647 .39S4 .4419 .3873 .4131 ------ 3.4406 3.6015 4.1833 3.2929 3.7819 3.Slel -----------------------m------------------------------- .30 3$IS .3765 .7145 .3811: .3S36 .3370 .0971 .30 8 .3365 .3396 .6314 .3S31 .3626 .3642 .0485 .2966 .2328 .3023 .3sis .2917 .3532 S219 .63S2 Ln w HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT I WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL OEATH-2;SUBSET-ALL ORGAN ABBREVIATION: ON - BRAIN, Ll - LIVER. LU - LUNG TEO - LF. TESTIS. TEI - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE GROUP ANIMAL NUMBER TERMINAL BODY WT (9) - - B-R - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -so- - - - - - - - - - -m--m m m m m m m -2-----C-67-5-3-9-------5-1-0-.-7---------.-39-1-6------3-,-4-26-4--------.3-1-4-6-------.-3-4-S3--------.3-5-1-2--------BS-4-6---------------------- 2 C6?540 2 C67S42 SS2.7 432.9 .3los .4552 4 4642 3.4591 .3360 .3641 .2984 .38'3S .2270 .3933 .5470 S244 2 C6?545 2 c67s46 436.6 476.9 .4099 .4436 3.66S9 3.39S9 .3575 .3226 .4014 .3541 .4165 .3S41 .?2SO .5679 2 C67547 2 C67554 464.4 496.4 .4534 .4365 3.3041 3.9091 .3SS4 .3254 .3397 .3439 .3560 .3378 .5446 .7233 2 C675s? 2 C67seo 505.0 472.1 .4169 .4322 3@3723 3.1012 ".3424 .3358 .3S34 .3168 .3606 .3261 .7139 .7796 - -- -- - -- --- -- NUMBER IN-GROUP: MEAN: STANDARD DEV: -is - 484.0 36.6 15 .4304 .0298 Is 3.6394 .3334 -is - - - - Is- - - .32ss .3501 .0281 .0283 -15 - .3SO3 .0323 is- - - - - - - --- .6419 .0139 -------------------------------------------------------------------------------------------------------------- m 3 C67S70 m 3 C67578 442.7 466.6 .5079 .4389 4,OISS 3.7604 .3169 .3236 .4155 .3803 .4042 .3S72 Igsg .6991 3 C67510 441@8 .4714 4.3732 .3466 .2385 .3543 .8330 m 3 C67581 483.8 .3996 3.1398 .3530 .3S30 .3SIO m 3 C67582 m 3 c67s83 45?,3 453.8 .4757 .4462 4.0798 4,1502 .3409 .3383 .3449 .6942 m 3 CGIS84 497.1 .4036 4.8627 .3093 .3635 .3133 .4094 .3111 .3855 .7169 .7103 m 3 C67S$S m 3 C61586 450.7 413.7 .5032 4.2909 .3343 .3451 .3S26 6803 m 3 colsol 493.0 .4605 3.8242 3 9650 .3S41 .3171 .3869 .3441 .3809 .8102 m 3 C67594 523.8 .3937 4,0656 .3111 .3409 .3434 @7413 m 3 C67602 504.0 .4162 4.0099 .3203 .3812 .3735 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT I WEEK SACRIFICE PERCENTAGES ----------------------------------------------------------------:----------------------------------------------- TA13LE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL OR - BRAIN Ll LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS.- TEI - MT. TESTIS. SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT 19) - - O-R - - - - -Li- - - - - -LU - - - - -TE-O - - - - -TEI- - - - -so- - - - - - - - - - ---- ----------------------------------------------------------------------------------------------------------- m 3 C67605 N 3 C67612 47S.9 469.9 .4391 .4390 3.S269 4.1782 .288S .3339 .3716 .3003 .6130 m 3 c676l4 489.4 .4222 4.0476 .2980 .3551 .3311 .3608 .3306 .6271 .7288 NU-MB-ER - -IN -GR-OU-P: - - - - -i-s - - - -Is- - - - - -Is- - - - MEAN: 470.9 .444S 4.1240 STANDARD DEV: 20.6 .0349 .3702 15 - - - - -Is- - - - .3261 .3S30 .0246 .0429 Is- - - - - 1-5 - - - .3592 .7340 .0242 .0900 ---------------------------------------------------------------------------------------------------------------- m 4 C67617 N 4 CG?629 421.1 SOS-2 ..44166247 5S..32781021 ..3231753 .3712 SS48 .1406 N 4 C67534 m 4 C67638 475.1 .4604 S.1178 .39169 .3SOO .3640 .3?S2 .3837 .5654 .6402 N 4 C67639 m 4 C67642 C67644 488.9 479.0 504.7 456.2 .4412 .4289 .4261 .4411 5.4280 S.6009 5.9938 .3290 .2936 .2924 .2996 .412S .3416 .3050 .4201 .34ss .6563 .0704 .6096 N4 N 4 C67646 478.6 .4333 4.7132 S.0612 .2743 .3621 .4067 .3SI2 .4030 .3S97 .7070 .6480 m 4 C67647 m 4 C67655 432.6 479.5 .4171 .4386 4 5227 4.2373 .3033 .3378 .3741 .3923 .6310 m 4 C676S6 465.4 .4386 4.607S .2984 .3353 .4084 .3298 .4210 .7518 SI41 m 4 C676S7 m 4 C67661 469.9 412.9 .4616 .5217 6.1614 4.3382 .3404 .3448 .4280 .3703 .4062 .3693 .697? .6222 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT I WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLWES: SEXnALL;GROUP&ALL;WEEKS-ALL DEATH-2:SUBSET-ALL BR - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS.- TEI - RT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL ----G-R-O-U-P--N-U-M-B-E-R--B-O-D-Y-W-t--(-9-)-------O--R--------------L-i---------------LU--------------T-E--O ------------T--EI-------------S-o-------------------------------------------- * 4 C07662 * 4 Coles? 431.7 494.S .4782 .4369 4S.2613652 .31 1 .3sel .3sol .7435 .2971 1 .3381 .3SOO .7127 NU-M1-3ER- -IN -GR-OU-P: - - - - -Is- - - - -I-s - - - - -Is- - - - -Is- w MEAN: 466.0 .4SIO S.1314 - - - -is- - - - -Is- - - - - -Is- - - - - - STANDARD DEV: 28.7 .0274 S317 .31 o2?91 3682 -.0342 .38si .0598 .6720 .072S -m-----s ----C-6-70-8-0-------43-0-.-9--------.-w4-6-7--0-------7-.-O-S3-2--------------------------------------------------------------- m 5 C67099 m5 451.9 .4314 5.2412 .3599 .3038 .3617 .395S .3531 .3707 .6585 ssoo CS??02 m 5 CS?703 402.8 473.9 .4263 .4461 5.4021 S.7472 .371S .3s .33S7 .3274 .6711 m s C0770S m 5 C67707 486.1 49?.S .42$S 5.4401 .30:2' 3080 .3404 .3541 .3271 Gsoo S326 m s COY?01 SOS.$ .4410 .4363 6.0750 4.8123 .3329 .31SS . 542 .3171 .3604 .3147 .6320 m 5 Col?09 m s C67710 364.7 453.1 SI43 .4S93 6.3762 .37of .44s9 .4271 SI71 .6418 m s CO?711 m 44$.S .4443 0.9046 S.2820 .3241 .20SY .4072 .4092 .370S .4082 .6621 .5573 5 Collis m s COYIIO 479.7 476.3 .4484 .4570 7.1014 6.4998 .327 .32 6 29$S .3169 .6114 s CS?721 4SI.0 46is 6.3174 .313930 -.3371 .41so .3405 .4057 .6145 .6312 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A, APPENDIX D INDIVIDUAL ORGAN-TO-BODY WEIGHT PERCENTAGES I WEEK SACRIFICE --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX*ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSETeALL OR - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION:TEO - LF. TESTIS-, TEI - AT. TESTIS, SO ACCESS/SEXONG SEX DOSE ANIMAL TERMINAL - - - - - - - - - -- -- --- ----GR-O-U-P--N-U-M-B-E-R-B-O-D-Y--W-T-(-9-)------O-R------m-----L-----------------L-U --------------TE--O------------T--E ----------So------------------------- m 5 C67722 429.8 .4600 5.8210 .3506 .3818 .3890 .6869 5 CS??24 472.1 .4413 6.73S3 .3345 .3713 .3624 .6947 -- N@HiEn ImN -GR-04J-P:- - - - -is- - - m -I- - - IS- - - s- - - - - I-5- - - Is m - - -is- - - - - MEAN: STANDARD DEV: 459.0 34.2 .4ses .0374 S.OS$7 .7118 .3321 .0415 .3699 .0404 .3620 .0342 .822S .0562 -----M ---m m a m 6 Na 14 a m a 6 6 0 m 6 m 6 m 6 m6 m6 ------------------------------------------------------------------------------------------------------ C$772$ 438.1 .4S03 3.7492 .3162 .3681 .3774 .8369 C67729 400.1 .4425 4.30SA .3227 .3761 .3646 .8743 C$7731 C67734 44$.S 416.S .4512 .4987 3.6101 3.7312 .3s?3 .3489 .3837 .3691 .3901 .3659 .7841 .7299 C67737 460.S .4700 3.4S47 .3S$S .3404 .329S .6921 C$7739 46?.4 .4563 3.43SO .3009 .3145 .3084 .4301 CO?743 438.9 SIS2 4.0221 .3862 .3703 .3413 s2ll cal7so 441.0 .4419 3.4494 .3018 .3152 3-OOSSEX S$43 C$7752 490.0 .4449 3.8514 .3233 .3007 .3S42 S431 C67IS3 475.7 .4191 4.4927 .337S .3559 .3688 .6091 Col?Sl 445.6 .4032 3.2883 .3571 .3SSI .3691 .6s7s C67763 C$7765 470.9 439.3 .4241 .4910 3.9303 3.7493 .3404 .3974 .3674 .3S32 .3675 .3S2S -Sit$ .66si CONTINUED ON NEXT PAGE 40 HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-70-BODY WEIGHT I WEEK SACRIFICE PERCENTAGES --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL BR - BRAIN Ll LIVER. LU - LUNO ORGAN A68REVIATION: TEO - LF. tiESTIS-. TEI - OT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL GROUP NUMBER * 6 cells$ * 0 CSYIGI NU-MB-ER - -IN -GR-OUP-: - MEAN: STANDARD DEV: TERMINAL BODY WT 19) - - O-R - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - - ------ --------- --------- -------------------------------------------------- 392.4 437.3 .4994 .4734 3,1603 4.0648 .3702 .40se .40s$ .4178 .3912 .4032 .7279 .6232 - - -Is- - - - -Is- - - - - -15 - - - - -Is- - - - - -Is- - - - -14- - - - - -Is- - - - - - - - - - 449.3 .46s4 3.7548 .3483 .3636 .3641 .6439 24.9 .0292 .370S .032S .0279 o2s4 .1000 ---------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-70-BODY WEIGHT 14 WEEK SACRIFICE PERCENTAGES ----------------------------------------------------------------------------------------------------------------- TABLE INCLT#DES: SEX*ALL;GROUP-ALL;WEEKS*ALL DEAT"-T:SUBSET-ALL ON - BRAIN Ll LIVER. LU - LUNG ORGAN ABOOTEVIATION: TEO - LF. fiESTIS-. TEI - WT. TESTIS. SO - ACCESSISEX ORO SEX DOSE AN114AL TERMINAL GROUP NUMBER BODY WT 19) - - o-n - - - - -Ll- - - - - L-U - - - - -TEO - - - - -TEI - - - -so - - - - - - - - - - --------------------------------------------------------------------------------------------------------- N I C67454 m 1 CG?459 569.7 495.8 .3830 .4315 3.6509 3.1676 .2190 .3031 .3091 .0219 m I C67460 m I 531.0 .4539 3.9455 .3086 .3152 .3041 .3041 .3674 .3168 Sla? .7792 C67462 1 C67470 596.7 Sig.? .3S29 .3930 3.0111 3.1804 .2497 .2921 .2915 .2922 .7139 m I CS?473 m 1 C67475 aio.s .3264 3.9254 .2S 2 .3482 .2149 .3446 .2922 .6515 .5773 m I C67482 567.4 511.8 .4094 .4198 3.5970 3.1146 .31S03 .3241 .33SI .3714 .3379 .3712 .6213 m 1 CG?4$S m 1 C67487 595.6 523.4 .3720 .4548 3.S413 3.3384 .3333 .326 3210 .6243 .4994 m I CS?418 m 621.2 .3687 3.6161 .3000 .2856 .327,? .2063 -.3203 .2613 .1004 .6423 I C67499 m I CGISOI S77.0 511.0 .3820 .4271 3.2060 3.7024 .2853 .3148 .3147 .3124 .6690 m 1 C67SO4 1 C6750S 600.3 548.8 .35S4 3@sist .30 7 .3000 .3029 .2991 .2971 .6461 .43SS .3$Sl 3.5850 .2765S .2941 .2967 .0001 NUMBER IN-GROUP: MEAN: STANDARD DEV: - - - -Is 558.7 41.1 -15 - - - - is- - - - -15 - .3944 .0377 3.5293 .2101 ..0229319, Is- - - - -Is - - - - IS- - - - - - - - - - - 3162 -.0292 .3100 .0296 .6327 .0937 -m----2-2----CC-067-75-15-10-7-----S4-26-77-..-02--------.-59-4-3-----3-.-2-2-19-------.-2-01-0-------.3-7-0-2------.-3-6-2-3 ------.-5-18-1------------------------------ m 2 colS19 515.4 .4299 .4100 2.9996 3.2410 .2950 .3111 .3491 .2255 .36s3 .3544 .6729 m 2 C67521 m 2 C67523 SS7.6 613 6 .3946 .3SSI 3.0461 .3106 .284 .2945 .5914 .6266 m 2 CG?526 513.2 .4208 3.3956 3.0158 .2923 .2929 .30871 .3311 .3028 .3366 .5228 .5657 CONTINUED ON NEXT PAGE Am Ln HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 14 WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL,GROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL SEX DOSE GROUP ANIMAL TERMINAL NUMBER BODY WT 191 ------ ------- ORGAN ABBREVIATION: -- -- -- -- BR Li ON BRAIN Ll LIVER. LU - LUNG TEO Lf. F[STIS-. TEI AT. TESTIS. SO - ACCESS/SEX ONG LU TEO TEI so - - - - - - - - - 2 C67533 MISSING 2 C67535 m 2 CG?536 549.3 570.3 .3830 .3790 3.0970 3.2323 m 2 c67s4l m 2 c67s49 m 2 C67S51 562.1 609.5 S$3.4 .3802 .3708 .3011 2.9319 3.1424 2.9800 m 2 CG?SS2 010.2 .3313 3.9108 m 2 C67553 560.6 .37$S 3.2093 m 2 C67sso S32.3 .4247 3.1780 - -- -- -- -- - .3090 .2909 3 2S950 2 2 1700 1 919 3 161 2 Sao .3484 . 210 .21 9 .2991 .3074 .3411 .3169 .2863 .3414 .3307 .2710 .2990 .3129 .4231 .3168 .28?S .4448 s26S .6741 .0516 .7497 .624S .6421 .7003 N@HiElt IN-GROUP: - - - -14 MEAN: S55.1 STANDARD DEV: 42.0 14 .4037 OS13 14 - - - - -14 - - - - 14- - - - -14 - - - - 14- - - - - - - - 3.2358 .2192 .3124 .3295 .6273 .3001 .0329 .0304 .0392 .0841 -m-----3 ----C-67-S-0-3 ------$-3-1-.7---------.-33-8-7-----4-.-2-29-9-------.-29-4-6-------.2-$-S-o------------------------------------------------- m 3 c67s64 m 3 C67S?i 569.9 491.6 .3733 .4324 3.SIOS .27 5 .3201 .2706 .302S .0123 .6919 m 3 C67S96 m 3 511.S .4380 3 5340 3.9566 .28956S2 .27 .3988 .325 .3785 .874S 3200 C67sgs m 3 C67600 534.3 SOS.1 .3810 .3793 4.2060 4.1506 .3 .2964 31126 -.3118 .1930 .5382 m 3 CG?603 m 3 C$7604 609.4 .3562 3.6607 0'0 .2820 -.279691 .2 4 .2957 .2768 SO98 .5301 m 3 C67$GS 556.1 573 2 3814 3915 3.5855 3.1741 .3 so .208 2 .3424 .3091 .3384 .6115 m 3 cal6ol m 3 C67808 527.1 3894 3.3917 .179, 3472 .3117 .3456 .5693 .4814 m 3 C07610 SOO.4 S48.1 4183 4396 3.4469 3.3677 .30 17 .316s 1 .3703 .316S .3S3? .3172 .6404 .7303 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES . AMERICA, INC. MADISON, WISCONSIN U S.A. INDIVIDUAL APPENDIX 0 ORGAN-TO-BODY WEIGHT 14 WEEK SACRIFICE PERCENTAGES --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUPeALL;WEEKS-ALL DEATH-T;SUBSET-ALL ON - 811AINtESLI LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TIS.- TEI - AT. TESTIS. SO - ACCESS/SEX ONa SEX DOSE ANIMAL TERMINAL ----GROUP --N-U-MB-E-R---B-O-DY--W-T--(-9-1------- B-R - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -so- - - - - - - - - - --------------------------------------------------------------------------- m 3 Co?ell 3 C67613 529.2 S$3.2 .4128 .3773 3.S623 3.657S .2S$4 .2991 .3100 .2020 .3235 .7480 m 3 CSYSIS 529.4 .4099 3.123S .3044 .3S70 .2$SS .3512 .5311 .6210 NU-MB-ER - -IN -GR-OUP-: - - - - -Is- - - - -15- - - - - -15 - - - - -is- - - - - -is- - - - -i-s - - - - Is - - - - - - - - - - MEAN: STANDARD DEV: SS2.7 40.7 .3951 .0296 3.6883 .3238 .2156 .0329 .3234 .0343 .3189 .030S .0454 .1221 --------------------------------------------------------------------------------------------------------------- 4 Co?eis m 4 coldis m 4 C67621 m 4 CO?623 m 4 C67624 4 C6703S m 4 C67641 m 4 CG?643 m 4 C67645 m 4 C67050 N 4 C07652 m 4 COYS53 m 4 cals5i 586.8 596.8 607.0 470.7 536.? S44.9 S45.4 455.6 621.6 556.3 506.3 522.1 444.L .3712 .3629 .3037 .4476 .3932 .3780 .3S49 .4574 .3s7l .3832 .4129 .3S43 4$OS 4.4641 3,8248 4.1273 3.8111 4.9301 3.7197 4.5321 3.8659 3.9035 5.0706 4.1619 4.8739 4.3343 .2190 .2?42 .2810 .3361 .2760 .2175 .2367 .3813 .2120 .3110 .3003 .3070 .345S .3414 .2953 .2733 .3561 .3182 .3450 .2896 .3771 .3196 .3164 .3680 .3186 .3844 .3291 .2894 .2610 .3533 .3271 .3S$3 .3063 .3123 .3261 .3060 .3587 .31SO .3779 .637S .6634 .4112 .8017 .6310 ssll .4664 .1275 sits .6200 .1068 SY12 .7418 CONTINUED ON NEXT PAGE INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 14 WEEK SACRIFICE PERCENTAGES HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL:WCEKS-ALL DEATH-T;SUBSET-ALL ON - BRAIN Li LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS.- TEI - NT. TESTIS. SO - ACCESS/SEX ONG SEX DOSE GROUP ANIMAL NUMBER TERMINAL BODY WT (91 - - -on - - - - -Li- - - - - -LU - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - - ------------------------------------------------------------------------------------------------ 4 C$7668 m 4 C67669 484.9 S23.S .4191 .3931 2.9439 4.SSS4 .3147 .3021 .34$S .340S It@*iEnIN-GROUP: - - - -ts - - - -Is - - - - IS- - - - -Is - - - - IS' MEAN: S3$.2 .39S4 4.2106 .3018 .3327 STANDARD DEV: 56.8 .0401 ssoo .0348 .0322 .3494 .5991 .3397 .6943 - -- -- -- -- -- -15- - - - - is .332S .0391 .0321 .1240 --------------------------------------------------------------------------------------------------------------- m s C67671 sso.6 .4047 .4ss7 S.2s99 4..3704 .3122 .30SI .3s79 .3ass .3S12 .30so .6373 .72S$ 5 C67672 m s C67673 459.5 Sol.$ .46sy 4 . S33 .2996 .345S .340S .8743 t4 s C67617 s C67679 532.1 437.4 .3991 .4656 4 . 63234 0 4476 .2702 .3519 .3049 .4233 .3067 .4040 .4190 .6992 m 5 celese MISSING m s C67681 412.5 .5383 6.2434 .3410 .3736 .3933 .5780 m s C67091 40S.9 .4911 .5491 4.2406 S.. 646S .3200 .34as .3703 .4?IS .3471 .4470 .7109 .7900 m 5 C6769S m s C67697 315.9 4it'i .4389 0 . 3146 .3047 .3770 .3712 IS75 m s C67700 551.6 .3671 .3775 6 . 0944 s . 7178 .3178 .2882 .366S .3217 .3506 .3191 .7434 .7067 s COIlt2 m s C67714 513.8 41S.S SI92 6 8072 .3596 .4003 .4217 .7810 CONTINUED ON NEXT PAGE 64 00 HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 14 WEEK SACRIFICE PERCENTAGES ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEXoALL;(IROUP-ALL;WEEKS-ALL DEATHAT.SUBSET-ALL 13N - BRAIN Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - Lf. iES71S, TEI - RT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP NUMBE$T BODY WT (9) - - -OR - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - - ----------------------------------------------------------------------------------------------------- m 5 C67716 m 5 CB?720 464.7 658.1 .4747 .2948 4.593S S.S230 .3438 .2210 .3635 .2722 .3sl7 .2703 .6049 .669S N"ER IN-GROUP: - - - - 14 - - - - 14 - - - - 14 - - - - -14 - - - - 14- MEAN: STANDARD DEV: 410.1 79.0 .4466 .0113 S.4947 .8403 .3136 .0357 .3653 .0426 -14 - - - - 14- - - - - - - - - - - .3610 .0464 .0910 G946 ----------------------------------------------------------------------------------------------------------------- 6 8 m 0 m 6 m 6 m 6 m 6 6 *6 *8 0 m 6 14 0 CB?730 C67732 CO?736 C57731 Cal?40 C67744 C67746 C$7747 C67748 CO?749 C077s4 CO?755 Co??So 490.1 532.2 sis.s $23.1 466.7 503.7 474.3 S36.4 503.4 495.6 546.3 473.7 490.0 .4160 .4197 .4093 .4091 .4500 .4394 .4456 .3982 .4235 .4298 .3990 .4sso .4137 3.3362 2.9375 3.0733 3.2072 3.2611 3.2920 3,0198 3,8943 3.1?75 3,2790 3.3204 2.9481 3.3323 .2901 .2147 .3112 .2934 .3130 .3637 .2792 .3221 .3224 .310? .3030 .3068 .3418 .3460 .3417 .3729 .35s9 .3369 .3370 .3005 .3376 .3293 .3292 .3037 .3154 .3420 .3733 .3361 .3314 .3sl4 .34SI .3324 .2172 .3456 .3171 .3311 .3070 .3170 .6869 .4218 .6270 .6142 .063S .9288 .7229 BS92 .7394 SI26 .1060 .4120 .6136 CONTINUED ON NEXT PAGE 40 APPENDIX 0 INDIVIDUAL ORGAN-TO-OMY W11014T PERCENTAGES 14 WEEK SACRIFICE HAZLETON LABNA70RIES AMERICA, INC. 14ADISON. WISCONSIO U.S.A@ ---------------------------------------------.--.-.-.-.-.-.--.-----------------------.-.-.-..................... TABLE I#CLWES: SEX*ALL;GAOUP*Att;wEEXS-ALL DEAT14*T;SUBSEt-ALL BA - BRAIN Li LIVER, LU - LUNG ORGAN ABBRIVIATTO#: TEO - LF, F[STIS-, Tfl - At. TESTIS, SO - ACCESS/SIX ORO SEX DOS( AN114AL TERMINAL GROUP NUMBER BODY WT 191 ............... ....co)?So 495,1 Ce??67 S03.# - - OR - - - - - - ---Ll--- - -- - LU ..... - -TE-O - - - - - ifI' S0 -1....................................... 4256 .4410 3,is3s 3.2117 33se @3040 3153 .3366 @3iss .3474 .55?S .$OOS t4@*iERtu-0ioup: - - - ^15 . . . .is - - - - is. . . . .1 5 I*Atl: S03.S @42$0 3.2341 .31so STANDARD DEV: 23,7 .0111 @2274 .0247 Is' .3400 @0224 .3360 @0216 is - - - -- - - - - .$410 '1741 ................................................................................................................ HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 22 WEEK SACRIFICE PERCENTAGES ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL GROUP-ALL;WEEKS-ALL DEATH-U@SU'BSET-ALL DR - BRAINTESLI - LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. TIS, TEI - RT. TESTIS, SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP HUMBER BODY WT (91 - - B-A - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - - ----------------------------------------------------------------------------------------------------------------- * I C674S2 * I C6?453 * 1 CG?456 1 C67471 S$4.9 666.3 S64.9 653.8 .3169 .3209 .3797 .3392 3.1027 3 5489 3,0772 3.5280 .2$S9 .2784 .2820 .2478 .2910 .2961 .2497 .2683 .3092 .3137 .2496 .2741 @7601 .5313 .6312 .5906 I CG?472 m 1 Co?413 m 1 CG?420 m I C67493 * 1 CG?496 * 1 C67497 419.7 615.4 610.6 510.4 507.2 597.7 .4231 .3610 .3313 .400S .4021 .3744 2.9316 3.2167 3@2494 3.046S 3,1059 3.1798 .3033 .2547 @2681 ..2904 .2961 .2631 .3126 .2165 '2911 .3350 -3418 .2725 @322? .2829 .3060 .35SO .3627 .27$S .5064 .6107 .6332 .5732 .6369 .6587 N@HIER IN-GROUP: - - - -to - - - -10 - - - - 10- - - - -10 - - - - 10- - - - -10 - - - - 10- - - MEAN-. sit.1 .3720 3.2077 @2776 .2940 .3054 .0145 STANDARD DEV: 61.6 .0330 .1976 .0187 .0280 .0357 .0114 ----------------------------------------------------------------------------------------------------------------- * 2 C67501 * 2 C67513 S63 4 680.3 .3869 .337S 3.3S?l 3.isii .3112 .2801 .274S .2429 .2899 .2S20 .6772 .5771 * 2 C67516 678.8 .3375 2.91$3 .2876 .2775 .2107 .4147 * 2 C67517 * 2 Ce?520 * 2 CO?521 * 2 C67534 * 2 C67531 618.8 SSI.L 567.8 eto.2 692.8 .3675 .416L 3952 3S40 3307 3.0601 2.84S9 3.0417 2.8139 2.9137 .264S .3009 .2762 @2$GS .2820 .32so .3132 .3105 .3097 .2672 .3503 -3099 .3058 .2483 .6911 .7702 SS79 CONTINUED ON NEXT PAGE hO HAZLETON LA130RATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 22 WEEK SACRTFICE PERCENTAGES --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES@ SEX-ALL.GROUP-ALL,WEEKS-ALL BEATH-U@SUBSET-ALL ORGAN ABBREVIATION: at GRAIN Li - LIVER. LU - LUNG TEO LF. TESTIS, TEL - RT. TESTIS, SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY Wt (91 - - 1-3R - - - - -Ll- - - - - -LU - - - - -TE-O - - - - -TEL- - - - -So- - - - - - - - - - --------------------------------------------------------------------------------------------------------------- * 2 CSIS43 * 2 csys4l SAS.2 560.7 .4033 .3970 3,LOSO 3.2012 .3lS4 .2103 .2730 .3100 .2750 .3044 .4362 .6363 NU-MB-ER- -IN -GR-OU-P: - - - - -10- - - - -10- - - - - -10 - - - - -t-o - - - - -10 - - - - -10- - - - - -10 - - - - - - - - - - MEAN-. 601.1 .3727 3.0414 .287S .2904 .2912 .60si STANDARD DEV: St.1 .0311 .1717 .0169 .0266 .0306 .0989 ------------------------------------------------------------------------------------------------------- m 3 C67561 m 3 C67567 m 3 C67572 OL2 .8 640.3 553@3 .3638 .3641 .4022 3.0858 2.9ssy 3.3263 .2587 .2605 .3166 .2142 .2787 .2980 .2748 .2837 .2129 .6875 .5135 .5961 3 C67573 S26.7 .4073 2.6814 .2917 .3241 .3232 .695S 3 C67575 m 3 ca?s7o m 3 C67588 m 3 c67s89 m 3 c67s93 m 3 C67009 729@2 S17.0 S?t.? 630.8 709.S 730.9 .3120 .3653 .3823 .3467 .2903 .3091 3,0518 3.1342 3.1122 3.4552 2.8923 2.4730 @2461 .2776 .2763 .3388 .2199 .2011 .2719 .2293 .2632 .3201 .2634 @2403 .2104 .3025 .2740 .3361 .260S .2sss .4155 .6S20 .6240 S733 S2L9 .4438 NU-MB-ER - -IN -GR-OU-P: - - - - -10- - - - -10- - - - - -10 --- - - -10- - - - - -10 - - - - -10- - - - - -to- --- - - - - - - - - MEAN: 629.2 .3543 3.0162 .2701 .2711 .2874 .5714 STANDARD DEV: 73.4 .03gy .2176 .0408 .034S .02S9 .0179 ---------------------------------------------------------------------------------------------------------------- 4 CG?622 m A C67626 m 4 CG?621 S63.6 SOO.2 666.S .3736 .3685 .3320 3.7194 3.3224 3.2197 .2701 .2911 .2644 .3322 .33SI .2791 .35so .3384 .2900 .6977 @7667 CONTINUED ON NEXT PAGE 14 f@a HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BODY WEIGHT 22 WEEK SACRIFICE PERCENTAGES ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEAT"-U;SUBSET-ALL ON - BRAIN Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS. TEI - NT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT 19) - - O-R - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- m 4 C67637 4 C67649 4 C@7651 m 4 C67660 m 4 C6?664 m 4 C67665 m 4 C67670 529.9 601.S 614.9 SS7.8 694.3 609.1 620.S .404S .3553 .3479 .42S9 .3167 .3663 .3SBO 4.2704 2 9OS2 3.3762 3.7013 3.0173 3.104S 3.8750 .3188 .2SIS .2641 .3094 .2915 .2928 .2889 .2968 .2034 2929 .3110 .2424 .242S .3139 .3031 .3212 .2883 .3192 .2441 .2398 .3069 .6313 .6784 S291 .6$21 .6320 .6606 .6S2S NU-MB-ER- -IN -GR-OUP-: - - - - -10- - - - -10- - - - - -10 - - - - -10-.1- - - - -10 - - - - -10- - - - - -10 - - - - - - - - - - - MEAN: 601.8 .3647 3.4661 .2821 .21SO .2996 .6s$o STANDARD DEV: 51.2 .0320 .4278 .0214 .0431 .03?S .0504 ------------------ s C$7674 * 5 celsis * s C676is 5 C67683 m 5 C67684 m s C67094 m s C67701 m 5 COT704 --------w -----------------------------w ------------------------------------------------------ $5s.4 399.4 .3314 SS41 3.3007 3.9854 .2580 .4030 .2999 .43S$ .2912 .4205 S$72 1.1436 S$2.S .3737 3.5266 .2SS2 .3127 .3114 SS26 S93 0 420.9 59S.7 .3618 .4219 .3125 2.9441 2.8872 3.3611 .2599 .2962 .2979 .2636 .3371 .3091 .26S3 .3491 .2902 .6137 S047 .6911 S41.8 597.4 .3733 .33S7 3.5120 3,34S3 .3089 .2559 .3203 .2696 .3127 .2806 .8868 SI$2 CONTINUED ON NEXT PAGE HAZLETON MADISON. LABORATORIES AMERICA. WISCONSIN U.S.A. INC. INDIVIDUAL APPENDIX 0 ORGAN-10-BODY WEIGHT 22 WEEK SACRIFICE PERCENTAGES --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SIX-ALL;GROUP-ALL;WEEKS-ALL DEATH-U;SUBSET-ALL ORGAN ABBREVIATION: SR - 13RAIN Ll - LIVER. LU - LUNG TEO - LF. F[STIS. TEI - AT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY Wt 421 - - O-R - - - - -Lt- - - - - -LU - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - --------------------------------------------------------------------------------------------------------------- m s CGI?06 m s CGI?23 S?2.9 561.3 .3110 .3111 3.7639 3.0124 .2737 .2968 .2913 .36.39 .2230 .3473 Sol$ slsi NU-MBE-R - -IN -GR-OUP-: - - - - - 10- - - - - to-, - - - - -10 - - - - - 10- - - - - -10 - - - - - 10- - - - - -10 - - - MEAN: SS6.0 .3004 3.3642 .2006 .3210 .3167 .6792 STANDARD DEV: 60.8 .0624 .3538 .0447 .0499 .04SS .1950 --------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT 5 WEEK SACRIFICE RATIOS ----------------------------------------------------------------------------------------------------------------- TABLE ITICLLIDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-I;SUBSET-ALL OR - BRAIN S Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. tE TIS, TEI - RT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT fol - - O-R - - - - -Li- - - - - L-U - - - - -TE-O - - - - -TEL- - - w -So- - - - - - - - - - - --------------------------------------------------------------------------------------------- ----------- m 1 C674si m 1 CS?451 m 1 C$7461 m I C67464 m 1 CG?467 m I C67468 m I C6?476 447.6 375,7 409.0 425.9 420.5 398.0 419,9 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 7.8411 7.3980 7.6076 8.4267 6.682S 7.3446 7.6429 .7947 .6190 .7081 .7413 .7490 .7010 .0851 .0089 .1421 .7444 .7874 .7201 SS3$ .1913 .7967 .7287 @7779 Soso .7122 .8914 .1723 l@ilso 1.2929 l@3517 1.1420 I.3oss 1.2731 1.3948 m I CS?477 m I CG?416 I C67489 m 1 CG?491 m I C61492 I cslsoo * 1 C67502 * I COYS03 420,1 397.0 382.7 301.1 4SI.9 401.7 409.0 418.1 .1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 8.67 716 7. iss 0,9602 7.4406 10.7239 7.7640 1.1735 7,1147 " 273 .7293 .4138 .7413 .7848 .?102 .7310 .7903 .7220 .7308 .1000 .7740 .0007 .1451 .8218 .7931 .7134 7329 .8232 .7131 .14S3 .1462 .8136 .8143 1.2222 l@2231 1.2604 1.4071 1.4930 1.2315 1.2146 l@3189 N@MilER 110-GROUP: MEAN: STANDARD DEV: -is - 411.1 21,7 -15 - - - - 15- - - - -15 - - - - 15- - - - -Is-- - - 1.0000 7.8802 .7309 .7932 .7971 .0000 @9350 .0969 .0541 OS62 Is- - - - - - - - - - 1.2925 .091.6 --------------------------------------------------------------------w------------------------------------------- m 2 C67509 m 2 co7sio m 2 ce7sit m 2 COYS14 m 2 C67515 m 2 C67S24 464.8 390.4 399.2 440,2 396.0 376.6 1.0000 1.0000 1.0000 l@0000 1.0000 1.0000 9.0382 6.12S4 7.2271 7.6741 7.0426 7.7844 .1741 @7701 .7391 @IS49 .1010 .6493 .8954 .7182 .8222 .$SOL .1136 .64s2 .8822 .7889 .8207 .839L .1157 .655L 1.4S24 1,4986 1,4718 L 2664 1 1311 1.2404 CONTINUED ON NEXT PAGE 00 Ln MAZLETGN LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT 5 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL.GROUP-ALL;WEEKS-ALL DEATH-1 SUBSET-ALL ORGAN ABBREVIATION: OTReo --BRLAFI.N,,ESLIS- LIVER, LU - LUNG TI . TEI - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP "UMBER BODY WT 191 - - s-o - - - - -Li- - - - - -LU - - - - -TE-G - - - - -TEL- - - - -So- - - - - - - - - - ---- --------------------------------------------------------------------------------------------------------- N 2 m 2 m 2 N2 N 2 N 2 m 2 m 2 t4 2 C67525 C67529 C67530 C67S37 C67544 ceisso C6755S C67sss coiSS9 434.7 404.5 422@6 3S9.0 387.7 387.9 422.1 433,7 408.4 1.0000 1.0000 1.0000 1.0000 1.0000 1,0000 1.0000 1.0000 1.0000 9.4123 6.7050 0@9552 6.6614 6@4180 7.1019 7@$463 0.1900 1.8859 .823S .646S .7366 .7401 .72S4 .7765 .7860 ..7630 .7881 .0917 .8137 .7484 .1310 @7617 .7SSI @7484 .7719 .7694 @982S .7151 .7537 .8460 .804t .7450 .7430 .7521 @7701 1.4274 1.1734 1.2475 1.0442 L.2417 1.2891 l@37SS 1.2218 1.7347 M64L'n IN-GROUP: MEAN: STANDARD DEV: - - - -Is - - - -I-s - - - - IS - - - - -Is - - - - 15- - - - -15 - - - - is- - - - - - - - - - - 413.9 1.0000 7.6323 .7S@4 BoIS .7990 1.3224 34.S .0000 .9170 .0575 .0794 @0?42 .1743 ---------------------------------------------------------------------------------------------------------------- N 3 C67S02 m 3 C67ses m 3 C67566 m 3 cols6i m 3 C07569 m 3 C67574 m 3 C6?SYG m 3 C67577 m 3 C67590 3 Co?59L m 3 COYS92 3 C67595 433.8 390.4 421.7 397,0 389.8 396.8 387,9 363.6 391.4 379.3 341.8 414.9 1.0000 1.0000 1.0000 1.0000 1,0000 1.0000 1.0000 l@0000 1.0000 1.0000 1.0000 l@0000 9.4062 8.2721 9.3371 9.0602 7.7598 1.0723 9.2426 7,8286 7,6369 8.3413 6.43S# 9.5801 .7266 Isie .6996 .7003 .6465 .7009 .6951 .62SO .6S97 .7170 .5971 .7696 .0519 .7675 .8100 .764S .0375 .9332 .1564 .7693 .0891 .7899 .69S3 .9t62 .8424 .7154 .1127 .?GO4 .2571 .9046 .8531 lt02 .6710 .7735 .7052 .1905 1.5170 1.11so 1 2374 1.5()04 1.2919 t.6004 1.6870 t.t494 1.1435 1.4260 1.2188 L.7512 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT S WEEK SACRIFICE RATIOS ----------------------------------------------------------------------------------------------------------------- TABLE lt#CLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-I;SUBSETEALL ORGAN ABBREVIATION: OR BRAIN Ll - LIVER. LU - LUNG TEO LF. fESTIS, TEI - RT. TESTIS, SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WY 19) - - O-R - - - - -Li- - - - - L-U - - - - -71-0 - - - - -TEL- - - - -so- - - - - - - - - - - ----------------------------------------------------------------------------------------------------------------- * 3 CGIS97 * 3 C61sge * 3 C67601 380.0 407.5 480.6 1.0000 1.0000 1.0000 9.6983 10.7416 10.IS2$ .7227 .7927 .7908 .7664 .1173 .7745 .7774 .8732 .8046 1.3262 1.2363 I.S373 N@MIEN Iti-(IROUP: MEAN: STANDARD DEV: - -- -- - -- -- -is - - - -Is - - - - IS- - - - -Is - - - - IS- - - - -Is - - - - IS 391.4 1.0000 1.7711 .7063 .1064 .1095 1.3197 32.0 .0000 1.1310 .0573 .0739 .0644 .2163 ----------------------------------------------------------------------------------------------------------------- * 4 C07619 * 4 C67620 * 4 C6762S m 4 C67627 409,2 469.8 311.2 316.2 1.0000 1.0000 1.0000 1.0000 12.S?00 12.5$OS 9.4741 10.1623 .7452 .7367 .7340 .7390 .7507 .747S .7771 .139$ .7627 .7142 .7170 .1115 1.1459 l.s79o 1.2sl9 l@2513 m 4 C6?630 457.0 1.0000 13.2292 .1396 .7999 IIS4 1.4667 m 4 C$7631 321.1 1.0000 IO.Si$6 .677S .6662 ss9s 1.4069 m 4 C67632 m 4 CO?633 m 4 cole3o m 4 C61640 m 4 CG?841 m 4 C67654 m 4 CO?659 316.5 453.7 3$S.3 397.0 392.0 333.5 416.0 1.0000 l@0000 1.0000 1.0000 1.0000 1.0000 1.0000 9,S175 11.7787 10.8271 12.0779 10.1241 10.6253 11.3720 .7052 .6023 .7501 SSS9 OS?o .6304 .1603 .1310 .7974 .7415 .784S .7729 .7180 .6943 .7900 .7915 .7160 .7980 .7334 .7800 .6842 1 2403 I'Slio 1.2247 1 4ess 1,2614 1 3$Ss 1.0622 CONTINUED ON NEXT PAGE INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT 5 WEEK SACRIFICE RA710S HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SIX-ALL;GROUP-ALL;WEEKS-ALL DEATHol;SUBSET-ALL OR - BRAIN Ll LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS.- TEI - NT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL (IROUP NUMBER TERMINAL BODY WT (91 - - O-R - - - - -Li- - - - - -LU - - - - -TE-D - - - - -TEI- - - - -So- - - - - - - - - - - ---- -----------;------------------------------------------------------------------------------------------------ 14 4 CG?663 14 4 CG?66S 413.9 387.1 1,0000 1.0000 10-4422 13.147S .7399 .7524 .8070 .1724 .9174 .1174 1.1731 1.2091 t4@OiE-R -1"-(I-Ro-up-: MEAN: STANDARD DEV: is 403,3 3S.5 I-S 1.0000 .0000 Is 11.2764 1.3499 1-5 .7242 .04S2 is.7713 .0513 -is .7679 Oslo is1.3096 .1497 --------------------------------------------------------------------------------------------------------------- s C676TS 408@9 1.0000 1.0000 14.0242 12.1795 -.'7040 .7iss .7517 .1030 .7761 .790S 1.34so 1.3391 m s calsoi m s C67612 372@9 373.0 1.0000 12.5501 .6430 .7162 .74ss 1.3217 m 5 C67617 313.1 392.4 1.0000 1.0000 10.3078 13.7341 .6471 .717S .7100 .7191 .?$Sl .7183 1.1043 1.1184 m 5 C67689 5 C67690 36S.2 1.0000 12.SS44 .7100 .7144 .7883 1.2441 s ceiGO2 390,2 1.0000 12.7203 .7904 .1397 .8358 1.37se 14 s CGTG23 C67606 402@O 3so.6 1.0000 1.0000 10-9615 13.0942 .0080 .6612 .7484 .57so .7354 .8216 1.5244 1.2490 s m 5 C67698 389@6 1.0000 11.9600 .6771 .8282 .8089 1.4213 m s C67713 m 5 CO?71S C67717 373,3 307.1 361.0 1.0000 1.0000 1.0000 11,5777 13.0609 12.13S26 .0547 .7118 .8720 .7426 .89is .7940 .7334 .9300 .1122 1.1262 1.3943 1.1111 m s m 5 C67725 346.6 1.0000 11.9117 .6905 .9117 .8904 1.3712 NU-14B-ER- -lf4-GR-OU-P: MEAN-. - - - -14- 374.4 - - -t-4 - l@0000 - - -14 - - 12.4713 - -14- - - .6925 - -14 - - .7734 - -14- - - .7971 - -14 - - 1.2900 - - - - - - - - STANDARD DEV: 11.4 .0000 1.0996 .0383 OIS2 .0604 .1328 ---------------- ----------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX 0 ORGAN-TO-BRAIN WEIGHT S WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-I;SUBSET-ALL OR - BRAIN Ll - LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS. TEI - RT. TESTIS, SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP "UMBER BODY Wt 191 - - O-R - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- m 6 C67126 m 6 CG?727 N 6 C67133 m 6 C6??3S m 6 C67141 m 6 Co?142 m 6 C6774S m 6 C67751 m 6 C67757 m 6 C677sg m 6 C67761 m 6 C67762 m 6 C6?164 m 6 Co?769 m 6 C67770 371.4 388.9 384.1 383.7 393.S 386.0 396.0 387.0 403.1 402,3 391,9 381.1 392.0 402.7 362.S 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1,0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.1216 7.5986 2.1327 s@8639 1.0070 8.3224 7.s4sg 6.9364 7.4613 7.3523 7.4714 8.0920 4.3136 1.1825 G.sosG .7247 .8120 .13TS .8046 .7147 .8606 .7546 ..Isle @7052 .5739 .613S .7691 .4220 .8220 .74SS .642S .7993 .963,7 .7151 .1492 .8758 IIS2 .7063 .7423 .7645 .7123 .1097 .1232 .801S .7799 .6139 .7650 .9671 .7640 .6932 .8723 IGSO .7282 .7474 .7173 .7691 .7727 .8230 .8206 .?371 1.2409 1.4075 .8190 1.1531 1.4363 1.4121 1.2011 1.2166 1.3341 .9517 1.3033 1.2057 1.2312 1.0738 1.3024 NU-MB-ER - -IN -GR-OUP-: - - - - -Is- - - - -Is- - - - - -is- - - - -is- - - - - -Is- - - - -i-s - - - - -Is- - - - - - - - - - - MEAN: 318.9 1.0000 7.86s6 .7631 .0000 .7951 1.2333 STANDARD DEV: 11.1 .0000 .69so .0650 .0767 .0771 .1674 ---------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT I WEEK SACRIFICE RATIOS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;OROUP-ALL;WEEKSPALL DEATH-2;SUBSET-ALL OR - BRAIN Ll - LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. fiESTIS. TEI - NT. TESTIS. SO - ACCESS/SEX ORO SEX DOSE ANIMAL GROUP HUMEN TERMINAL BODY WT (91 - - O-N - - - - -Li- - - - - -LU - - - - -TE-O - - - - T-EI- - --- -so- - - - - - - - - - - ------ww -----------------------------------------------------w--------------------------------------------------- m I C6745S 403.1 1.0000 7.1517 .8302 .7811 .8023 1.3949 I C6?4S$ 450.9 1.0000 7.8221 .7473 .7072 .7744 1.2432 * I C$7463 412.6 1.0000 7.4175 .7904 .7418 .704S I.SO04 * I CS?465 422.1 1.0000 7.3430 .0083 .#528 .8204 1.1027 * I C$7466 * I CO?469 420.3 494.0 t.0000 1.0000 9@2?SS 10.2140 .6870 7702 .7433 .8004 .7766 .8276 l.s2s? 1.4569 * 1 CG?472 CO?474 S16.8 478.1 1.0000 1.0000 10.416S 1.5124 .?OIS .. .7638 .9772 .8370 .9121 .1681 l.asod 1.6572 * I * 1 CG?479 474.1 t.0000 8.1294 . 7824 .1176 .8021 1.6196 * I CS?480 4S9.0 1.0000 ?.?SI4 .6522 .1110 .1129 1.332S * 1 C67411 * 1 C67424 43S.L 416.7 l@0000 1.0000 7@3869 I.ssls .73S7 .8320 YS$3 .8292 .8019 .8411 1.2064 1.4172 * I C67494 4S2.1 1.0000 7,6217 .7soo .75SS .7480 1.3724 1 C67495 415.S 1.0000 8,9647 .8250 .8269 .8510 l@7215 m I C6?491 S06.0 l@0000 9.9701 .7345 .7314 .7301 I.S413 NUMBER IN GROUP: MEAN: STANDARD DEV! -Is - - - -is - - - - -is - - - -is- - - - - -Is- - - -Is- - - - - -Is- - - - - - - - - - - 473.1 1.0000 8,4900 .7571 .8042 IIS3 1.4672 26.0 .0000 1,06$S .0530 .0614 .0608 .1780 --------------------------------------------------------------------------------w ------------------------------ m 2 CS?506 m 2 C67siz m 2 C$7522 m 2 C$7527 m 2 C67S31 m 2 C67S32 441.4 440.1 525.9 49S.1 524.8 494.2 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 7.4858 7.IS02 10.S?99 7.3362 9.76ss 1.6823 .7860 .8217 .7033 .7808 .7658 .6846 .8287 .7609 .8509 .8079 .7805 .8508 .1177 IZS2 .8589 .8113 .7533 .8550 I.SS18 1.5001 l.s969 1.4447 1.3475 1.5376 CONTINUED ON NEXT PAGE 04 HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT I WEEK SACRIFICE RATIOS ------------------------------------------------------------------------------------------------------------ TABLE INCLUDESSEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL ON - BRAIN Ll - LIVER. LU - LUNG ORGAN ABBREVIATION TEO - Lf. TESTIS. TEI - NT. TESTIS. SO - ACCESS/SEX OftG SEX DOSE AN114AL TERMINAL - -- -- -- -- -- -- - -- -- -- -- - -- -- -- --- --- -- - -- GROUP NUMBER BODY WT 191 BR Ll LU TEO TEI so ---------------------------------------------------------------------------------------------------------------- m 2 C67539 m 2 c97s4o m 2 C67542 m 2 C67545 m 2 CO?546 m 2 C57S47 m 2 C67SS4 m 2 CG?SS7 m 2 C67560 510.7 SS2.7 432.9 436.6 476.9 464.8 496.4 SOS.0 472.1 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.5902 11.7314 7.5997 1.6010 7.OS47 7.2174 9.OS70 8.0214 s.se4i .7094 .8245 .10Is .7608 .7212 .7831 .74S4 .8213 .:7770 'le$4 .7842 .:41S .6 1 .1982 .1492 .7871 .2474 .7794 .1112 .720S .1640 .1963 .7982 ISS2 .7740 .1649 IS46 1.6423 1.4376 1.1521 1.5427 1.2101 1.2011 1.6sit 1.7122 1.1040 NU-MB-ER- I-" -GR-OU-P:- - - - -is- - - - -is- - - - - i-s- - - - -Is- - - - - -Is- - - - -Is- - - - - -Is- - - MEAN: 414.0 1.0000 S.S2S3 .7799 .1136 .$140 1.4939 STANDARD DEV: 36.6 .0000 1.21?7 .045S .0305 .0sly .1863 ---------------------------------------------------------------------------------------------------------------- m 3 C$7570 m 3 c67s78 m 3 C67580 m 3 cslsli m 3 C67512 3 C07583 m 3 C67584 m 3 CS?SIS m 3 co7sos m 3 C6?S87 m 3 C57SS4 m 3 C67002 442.7 466.6 441.1 483.1 4S?.3 453.8 497.1 4SO.7 413.7 493.0 S23.8 504.0 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 8.0444 I.Se$7 9.2173 9.6080 10.2560 9.3011 12.0489 9.S492 7.6002 8.6096 10.3275 9.6338 .6239 .7373 .7352 .1832 .7166 .0932 .9001 '7441 7037 .6215 .7920 .769S Iiso .1666 sosi .8833 .7111 .7022 1.0145 .7680 .7669 .7472 IGS9 .91so Tosl .1139 Isis .1704 .7251 .6973 .9551 .7840 .7570 isso .8724 .8974 1.7439 l.s930 1.7612 1 7167 1.4592 1.7636 1.7600 l@5140 1 7266 1,7594 1.9009 1.4412 CONTINUED ON NEXT PAGE INDIVIDUAL APPENDIX 0 OROAM-70-BRAIN WEIGHT I WEEK SACRIFICE RATIOS HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKSmALL DEAT#4-2;SUBSETPALL OR - BRAIN Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. iES71S. Itl - RT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL - - O-R - - - - -Li- - - - - L-U - - - - -YE-O - - - - -TEI- - - - -So- - - - - - - - - - - ----GR-O-U-P---NU-M-B-ER---B-O-D-Y---W-Y---(-9-) ------------------------------------------------------------------------------------ 3 C67606 475.9 1.0000 $.oils 9.51!3 BIOS .7806 .1440 .2019 .$874 @$220 1.39se 1.4216 m 3 CS?612 3 C67614 469.9 489.4 1.0000 1.0000 $.So I .7os7 .7843 7830 1.1262 li@mi[R lt4CRoup: -is - is Is- - 9.3301 I$ .7377 Is- .1004 is - - - - Is- - - - - - - - - - .8117 l.6s24 MEAN: STANDARD DEV: 470.9 28.0 1.0000 .0000 111031 .079S .1154 .0727 .1631 ------------------------------------------------------ ---------------------------------------------------------- m 4 CO?617 428.8 1.0000 11. 12 .7103 .8022 1.2206 1.8166 4 CG?629 505.2 1.0000 12.491,Sa .7139 .8882 .8563 .7906 .2010 .1334 I.3s7# 1.4101 m 4 C67634 m 4 C67638 4 cals3o 475@l 480.9 4?9@o 1.0000 1.0000 1.0000 1,0000 11,2614 12,1129 13.0588 14.0069 .73ss .6846 .6853 .$$Is .9510 .1018 .6805 .9$11 .$to$ 1.4644 I.SG31 1.4301 m 4 C67642 m 4 C67644 S04-7 456 2 l@0000 10.6162 .8218 .9220 .9137 8300 1.6029 1.4953 m 4 c67646 470.6 432.6 1.0000 1.0000 it. 59 9.629ls9 .8356 .74S9 .2267 .7682 .8054 1.29SS m 4 C67647 m 4 C67655 m 4 C67656 479,5 465@4 460.9 0000 I1..0000 1,0000 11.2SS7 lo.sos: 13.362 .7702 .8804 .737S .764S .9312 .9272 .7519 .9600 .6800 1.713$ 1.3tO6 1 5114 m 4 C67657 m 4 C6766L 412.9 1,3155 .0608 .7097 .7071 1.1926 mm CONTINUED ON NEXT PAGE HAZLETON LABORA70RIES AMERICA, INC. MADISON. WISCONSIN U.S.A. INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT I WEEK SACRIFICE RATIOS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUIRSET-ALL OR - BRAIN Ll - LIVER, LU - LUNG ORGA14 A813REVIATION: TEO - LF. TESTIS. III - MY. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANlt4AL TERMINAL -- -- --- - -- -- -- -- -- - -- - -- -- -- -- - -- -- -- --- GROUP NUMBER BODY WT 191 OR Ll LU TEO TEI so --------------------------------------------------------------------------------------------------------------- 4 CS?062 431.7 1.0000 9.7330 '6679 .7S33 .7351 m 4 C67661 494.S 1.0000 11.9378 '6769 .7736 .8024 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - --- - - - - - - 1.5613 1.6311 --- - -- -- -- -- NUMBER IN GROUP: MEAN: STANDARD BEV: Is 466 9 28,7 is 1.0000 .0000 Is 11.4408 l.slos Is .70$3 osig Is .8172 .0$64 is IS43 .1342 Is 1.400S .1662 ----------------------------------------------------------------------------------------------------------------- s C67680 s C67699 s C6?702 s C67703 5 Co??05 t4 s Cal?07 m 5 CS?708 m s C67709 m 5 CS?710 m s cellit m s C67718 m s C67719 m 5 C67721 430.9 451.2 462.1 473.9 486.8 49?.S SOS.$ 344.7 4S3.1 441.S 479.7 476.3 4SI.9 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 l@0000 1.0000 1.0000 1.0000 1.0000 IS.1040 li.osso 12.6714 12.8143 12.0960 13.7711 11.1894 10.9122 IS.0320 ll.lg7G 15.8362 14.2221 13.6890 .8298 .8714 .7999 .721S .7SSO .7230 .6407 .705S .4621 .7306 .7199 .6780 .7746 .9022 .7174 .82so .7944 .6076 .7283 .7931 .186S .9209 .0657 .7392 .8994 IS62 .8454 .7610 .795S .76so .1113 .7212 .7310 .1067 .9111 .7068 .74si .1191 1.4101 1.2741 1.5759 1.4787 1.2429 1.4332 i.ilso 1.0924 1.4431 1.2541 1.3?91 l@344S 1.3676 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. MADISON, WISCONSIN U.S.A. INC. INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT I WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL GROUP-ALL;WEEKS-ALL DEATH-2;SU* 13SET-ALL 13R - BRAIN Ll - LIVER, LU - LUNG ORGAN A13OREVIATION: TEO - LF. TESTIS, TEI - RT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT 19) - - -ON - - - - -Li- - - - - -LU - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - ---------------------------------------------------------------------------------------------------------------- 5 C6?722 429.s 1.0000 12.6217 .7780 .84)S .1440 1.4902 m 5 C6?724 4?2.1 1.0000 15.0256 .7463 .8212 .8086 l.s49? -- - -- -- -- -- -- --- -- -- - -- -- -- -- -- -- -- - -- -- - -- -- -- -- - -- --- - NUMBER IN GROUP: MEAN: STANDARD DEV: Is 459.0 34.2 Is l.ouuo .0000 Is 13.3008 1.51?S Is .7205 .0920 is .1111 .0722 Is .7939 .0604 Is 1.3615 .1361 -------------------------------------------------------;-.-:------------------------------------------------------ m a C67728 m 6 C67729 m 6 CB?731 m 6 CGY?34 m 6 C67?3? 6 C67739 6 C67?43 m 6 C$7750 m 6 C67752 m 6 C57753 m 6 C67758 m 6 CS?763 m 6 C6716S 438.8 460.8 44$.S 416.5 460.S 467.4 438.9 441.0 490.0 47S.7 445.6 479.9 439,3 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 8.1833 9.7305 S.0005 7.4061 7.3SO2 7.5273 7.8067 7.$Sl$ I.GS68 10,7203 0.6673 9.2S26 7.6268 .0884 .7292 .7919 .6990 .7628 .6seA .7496 .6830 .7268 .8052 .7241 'Sots .1013 .1015 .8500 IS03 .7401 .7242 .6191 .7117 .7134 .8107 .8422 .7200 .8650 .7116 .8217 .0240 .1644 .733? .7010 .67so .6761 6.8024EX .7961 .8799 .7483 lost .7170 1.8221 1.5239 1.7377 1.4637 1.4726 .9425 1.0113 1 3222 1.2207 1.5967 1.3330 1 2231 1.3529 CONTINUED ON NEXT PAGE OR co 4@ INDIVIDUAL APPENDIX D ORGAN-TO-DRAIN WEIGHT I WEEK SACRIFICE RATIOS HAZLETON LABORATORIES AMERICA, INC. "ADISO@4. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-Z;SUBSET-ALL 80 - BRAIN Ll LIVER, LU - LUMO ORGAN ABBREVIATION: TEO - Lf. TESTIS.- TEI - NT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE OROUP ANT14AL TERMINAL NUMBER 1300Y WT (9) - - OR - - - - -Li- - - - - L-U - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - --------------------------------------------------------------------------------------------------------------- m 6 C$7766 m 6 COYTOI 392.4 437.3 1.0000 1.0000 0.3270 I.S$71 .7412 .8572 .8127 .8826 .7973 Isis 1.4SIS 1.3les it@SiE-lno,-t(im-,o-u-p.-. - -i-s - - - -15- - - 1.0000 - -is- - - - -is- - - .7415 - -Is- --- .7131 -14- - - .7823 - -is- - 1.3804 - - - - - - - - MEAN-. STANDARD DEV: 449.3 24@9 .0000 1.1411 'OSSS .0672 .0727 .2390 --------------------------------------------------------------------------------------------------------------- HAZLETON LABORATORIES AMERICA, MADISON. WISCONSIN U.S.A. INC. INDIVIDUAL APPENDIX D ORGAN-TO-BRAIN WEIGHT 14 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL ORGAN ABBREVIATION: ON - BRAINTESLI - LIVER. LU - LUNG TEO - Lf. TIS, TEI - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY Wt (9) - - O-R- - - - -Li- - - - - -LU - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- I C67454 569.7 1.0000 9.5312 .75AS .7930 .8069 l@6236 m 1 CS?459 495.1 1.0000 7.3415 .7151 .8440 .8518 1.3598 I C67460 531.9 1.0000 1.6932 .6946 .0716 .6980 1.7169 m I C67462 596.7 1.0000 1.7330 .7076 .1451 .1281 2.0231 m I C67470 519.7 1.0000 9,8734 .7433 .8860 .0767 1.6576 m I C6?473 sio@s 1.0000 12.0263 .7150 .8721 1.7687 m 1 C6747S 567.4 1.0000 8.7157 .7702 .8116 .12S4 1 5347 m 1 CG?482 m I C67485 m I C87487 511.8 59S.6 523.4 1.0000 1.0000 1.0000 7.5861 9@S207 7.3411 .7720 :8960 .6611 .8147 .8787 .720S .8842 .$630 .1044 1,4872 1,3427 1.7733 m I CS?488 621.2 1,0000 9.0076 .7746 .7221 .7088 1.7421 I CG?499 577.0 1 0000 8.3947 .7470 .8234 .1180 1.7SI4 * 1 C67501 511.0 1,0000 8.6686 .7371 .7024 .7003 1.5127 * I C67504 600.3 1.0000 9.8928 .8531 IS24 .8359 1.225S 1 C67sos 548.8 1.0000 9.2949 7142 .7624 .7692 1.5165 - - -- --- -- -- -- --- - -- -- -- -- --- - -- -- - -- - --- -- - -- -- -- - -- -- -- NUMBER IN GROUP: MEAN: STANDARD DEV: Is 558.7 41.1 is 1.0000 .0000 15 9.0327 1.2.007 is .7557 .0009 is .8053 .0723 Is .8044 .0708 Is 1.6064 .2047 ---------------------------------------------------------------------------------------------------------------- 2 C67507 527.0 1.0000 5 4209 .4845 .6229 .6096 .9907 m 2 C67511 467.2 1.0000 6.9771 .6861 .8121 .8497 1 5052 m 2 C67519 SIS.4 l@0000 7.9051 .7587 .5500 .8645 1 4425 m 2 C67521 557.6 1.0000 7.7199 .7872 .7200 .7463 l,slio m 2 CS?523 613.6 1.0000 9.5627 .8233 .8693 .8521 1 4122 m 2 C6?526 513.2 1.0000 7.1660 .6960 .7868 .7998 1.3441 CONTINUED ON NEXT PAGE co crt HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ORGAN-TO-BRAIN WEIGHT 14 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL-,GROUP-ALL:WEEKS-ALL DEATH*T;SUBSET-ALL OR - BRAIN LIS- LIVER, LU - LUNG OROAN ABBREVIATION: TEO - LF. TEST . TEI - Rt. TESTIS, SO - ACCESS/SEX ONG SEX POSE ANT14AL TERMINAL OROUP NUMBER BODY Wt 191 - - O-R- - - - -Li- - --- - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - --------------------w------------w----------------------m ---------------------------------------------------- * 2 c67s33 MISSING * 2 C07535 S49.3 m 2 C67S36 570.3 m 2 C67S41 S62.1 m 2 C67S49 609.5 m 2 CG?SSI * 2 C67SS2 * 2 C67SS3 S$3.4 610.2 S60.6 * 2 co7sss 532.3 1.0000 1.0000 1.0000 1.0000 1.0000 .1.0000 1.0000 1.0000 1.067S s@5278 7.7119 10.3011 1.4736 11.8041 8.4797 7.4136 Ioso .7675 .8666 .7686 .6772 .5703 -:8351 6277 .9074 .8406 .7442 loss .7709 1.0297 .1373 .8742 .0075 .1725 .1328 .8003 .7040 1.2792 .8371 .0770 1.0797 1.3190 1.7?31 1.7570 1.8103 1 $$so 1.6965 1.6490 N@MIERIN GROUP: MEAN: STANDARD DEV: 14 - - -14- - - - - -14 - - - - -14- - - - - -14 - - - - -14- - - - - -14 - - - SSS.1 1.0000 8.1901 @7252 .7144 .8300 I.S795 42.0 .0000 1.5593 .1075 .1209 .1528 .2402 ---------------m ---------------------------------------------------------------------------------------- m 3 cels$3 m 3 C67S$4 m 3 c67s7l m 3 C67596 3 C67598 m 3 COYBOO m 3 C67003 m 3 C67604 m 3 C6760S m 3 C67607 m 3 C67601 m 3 C67610 631.7 569.9 491.6 511.5 534.3 595.1 609.4 556.1 573.2 527.1 500.4 S48.1 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 12.4871 9.4027 8,1736 9.0336 10@8400 10.9414 10.2780 9.4008 9,1969 I.YIOS 8.2394 7.6610 .8697 .7378 .6699 .6284 .7758 .7814 .7942 .8076 .7311 .4547 .7332 .7167 .1414 .1573 .9223 .7434 .1021 .7185 .77so .8977 .7894 .0916 .8851 .1201 .7990 .1103 .8754 .7307 .1037 .7796 .7770 .1873 .7962 .8875 .8454 .7215 1.1077 I.IS33 2 0227 2.0319 1.3170 1.5813 1.4111 1 6216 1 4S42 1 2363 1 S308 l@6612 CONTINUED ON NEXT PAGE c@oj HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ORGAN-TO-BRAIN WEIGHT 14 WEEK SACRIFICE RATIOS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-T; SUBSET-ALL 13R - BRAIN S Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. tE TIS, TEI - RT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT 191 - - -OR - - - - -Li- - - - - L-U - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - - - ----------------------------------------------------------------------------------------------------------------- m 3 C67611 m 3 C67813 m 3 C67615 529.2 583.2 529.4 1.0000 1.0000 1.0000 8.6475 9.6948 7.7666 .0503 .7946 .7427 .7511 .7491 .8710 .7137 .7SOO Isol 1.8122 1.4097 1.5164 NU-MB-ER - -IN -GR-OU-P: - - - - -15- - - - -Is- - - - - -Is- - - - -Is- - - - - -Is - - - - -is- - - - - -Is- - - - - - - - - - - MEAN: 552.7 1.0000 9.AIIS .7284 .1191 .9074 1.6211 STANDARD DEV: 40.7 .0000 1.3310 .0912 .0655 .0532 .2354 ----------------------------------------------------: ------------------w------------------------------------------- m 4 Ce?sle m 4 C67011 m 4 C87621 * 4 C67623 * 4 C67624 4 C6763S m A C67641 m 4 C6?643 m 4 C6764S m 4 C67650 518.8 596.6 807.0 470.7 S36.7 544@g S45.4 455.6 621.6 550.3 1.0000 1.0000 1 0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 12.0257 10,5392 11.3484 8,51ss 12.5377 9.8416 12.7716 8.4527 10.9111 13.2312 .7705 .7ss4 .7918 YS09 .7020 .7607 .8671 .8331 .7603 oils .9191 .0137 .7514 .795S .8092 .9129 .8161 .8245 .8934 .8257 .1865 .7975 .7386 .7192 .1311 .9506 .8632 .1359 oils .7984 1,7173 1.8210 1.1306 1.7909 1.6046 l@47as 1.3143 1.8094 1 4298 1.6177 m 4 C07652 m 4 C676s3 m 4 C87658 506.3 592.1 444.1 1.0000 1.0000 1.0000 10,0803 13.?SYI 9.0204 .7420 less .7190 .8864 .8994 .1000 .1688 .8190 .7864 1.9S3S 1.6122 1.5438 CON71NUED ON NEXT PAGE f%3 co co HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ORGAN-TO-BRAIN WEIGHT 14 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL BR - BRAIN Ll - LIVER. LU - LUNG ORGAN ABBREVIATION: TEO - LF. TESTIS. TEL - RT. TESTIS, SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL - --- -- - -- -- -- -- -- - -- -- -- -- -- -- -- - -- -- -- - GROUP NUMBER BODY WT 19) SN Ll LU TEO TEI so ---------------------------------------------------------------------------------------------------------------- m 4 C$7668 m 4 CG?669 414.9 S23.5 1.0000 1.0000 7.0144 11.689S .7497 .76?3 .0327 .8647 .832S .1027 1.4290 1.7831 NU-MB-ER- -IN -GR-OU-P: - - - - -Is- - - - -Is- - - - - -Is- - - - -15- - - - - -Is- - - - -Is- - - - - -Is - - - - - - - - - - - MEAN: S3$.2 1.0000 10.7811 .7632 .9430 .8428 1.6014 STANDARD DEV: so.$ .0000 1.9014 .0498 .0498 os5s .2198 ---------------------------------------------------------------------------------------------------------------- * s C67611 * 5 C67672 SSG.$ 459.5 1.0000 1.0000 12.9969 9.3041 .7714 .6564 .8944 .7849 .$ISO .1131 I.S?46 I.SS$4 * s C67673 * 5 C6?677 m s CGTGIS m 5 C67686 Sol.$ 532.1 437.4 MISSING 1.0000 1.0000 1.0000 9.9924 10.8141 13.6469 .6434 .6758 Tssl .7412 .7627 .9091 .7311 .7673 .8676 1.4480 1.2231 1.5016 t4 s C67668 412.S 1.0000 II.S994 @63S2 .6941 .1307 1.0731 m 5 celegi 405.9 1,0000 9.8559 esis .7S39 .7067 1,4476 m s C$7695 31S@9 1.0000 10.2835 6310 'IS$? .8141 1.4312 m 5 C67697 411.7 1.0000 14.3$SS .6943 .1589 .8611 l@9537 m s CGIIOO 558's 1.0000 16.5701 '1641 .9965 SS32 2.0213 s COYI12 S$3.8 1.0000 15.1461 .7636 .8522 .84S3 I $?go m 5 C67714 415.5 1.0000 13,2834 6925 .7710 .8122 1.5176 CONTINUED ON NEXT PAGE co %D HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. APPENDIX 0 INDIVIDUAL ORGAN-TO-GRAIN WEIGHT 14 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL GROUP-ALL;WEEKS-ALL DEATH-1@SUBSET-ALL ORGAN ABBREVIATION: OTREO --BRLAfI.NTESLI - LIVER, LU - LUNG TIS. TEI - RT. TESTIS, SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WT (91 - - -OR - - - - -Li- - - - - L-U - - - - -TE-O - - - - -TEI- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- 5 C67?16 5 C67720 464.7 658.1 1.0000 1.0000 9.0?64 18.7327 @7242 .?YS3 .76st .9232 .7401 .9187 1.2743 2.2707 NU-MBE-R - -IN -GR-OU-P: - - - - -14- - - - -14- - - - - -14 - - - - -14- - - - - -14 - --- - -14- - - - - -14 - - - 14EAN -. 419. 1 1.0000 12.612L .7096 .82ss .1154 1.5840 STANDARD DEV: 79.0 .0000 2.8123 .0686 .0849 o7s9 .3327 --------------------------------------------------------- m a C67730 m 0 C67732 m 6 C67?36 m 6 C57738 420.1 532.2 SL6.5 523.1 t.0000 1.0000 t.0000 1.0000 8.0202 6.9991 7.5015 7.9871 .7414 .7054 .6950 .7606 m 6 Cal?40 466.7 1.0000 7.2645 .0520 m a CS?144 m a ce?746 m 6 C67747 6 C67748 * 6 CO?749 * 6 C677S4 6 C67755 m a C677SG S03.7 474.3 536,4 503.4 495.6 S46.3 473.7 490.0 1.0000 1.0000 1.0000 L.0000 1.0000 1,0000 1.0000 L.0000 7.4923 6.7?64 9@7794 7.5027 7.8284 4.3416 6.4100 1.0551 .7125 .1160 .7012 .7623 .7501 .7930 .7979 .7416 ------m ---------------------------------------- .821? .1220 1.6513 .9197 .8895 i.ooso .1348 .8211 l.s3l$ oils .8100 I.SO14 .7909 .1109 1.474S .7669 .7853 2.1134 .7580 .7460 t.6223 .7S47 .7213 1.6555 .7972 .8159 1.74se .7062 .8250 .7377 .8297 1.3554 2.0200 .7994 .8067 1. 564 .7023 .7664 1.4132 CONTINUED ON NEXT PAGE to c@ HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ORGAN-TO-BRAIN WEIGHT 22 WEEK SACRIFICE RATIOS --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GRDUP-ALL;WEEKS-ALL DEATH-U;SUBSET-ALL so - ORAt" Ll - LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. FESTIS. TEL - RT. TESTIS. SO - ACCESS/SEX ORG SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODYWY 19) - - O-N- - - - -Li- - - - - L-U - - - - -71-0 - - - - -TEI- - - - -So- - - - - - - - - - --------------------------------------------------------------------------------------------------------------- m 1 c6l4s2 m 1 COYA53 m I C674S6 m 1 CS?411 I C67419 m I C67413 I C6?420 m I CG?493 1 CG?426 m 1 C67497 564.9 606.3 S64.9 653.8 489.7 61S.4 610.6 510.4 507.2 597.7 1.0000 1.0000 1.0000 I.DOOO 1.0000 I.ODOO 1,0000 1,0000 l@0000 1.0000 8.2525 ii.osts 8.1040 10.4023 6.9111 1.9113 9.8017 7.6069 7.7233 1.4933 .1390 .1071 .7428 .7307 Tisy .7057 .8092 -,.7402 .7363 .7026 .7S20 .9248 .6s76 .7910 .7315 .7938 .1809 .8364 .8499 .72?9 .7993 .9776 .6514 .8002 .7613 .7836 .923S .8864 .9018 .7438 1.9802 1.6713 1 $624 1.7414 1.1949 1.6920 1 9112 1.4312 1.5039 1,759S NU-MB-ER- -IN -GR-OU-P:- - - - -10- - - - -10- - - - - -10 - - - - -10- - - - - -10 - - - - -10- - - - - -10 - - - - - - - - - - MEAN: 57$.1 1.0000 6.7217 .7490 .7952 .2243 1.6634 STANDARD DEV: 61.0 .0000 1.3187 .0512 .0799 .0966 .22SS --------------------------------------------------------------------------------------------------------------- m 2 CGISOI m 2 C67513 m 2 C67SI6 m 2 C67517 * 2 C6?520 * 2 C67521 m 1^ C67534 m 2 C61538 563.4 680.3 oil's oil.$ ssi,i S67.8 610.9 692.6 1.0000 1.0000 1.0000 1.0000 1 0000 1.0000 1.0000 1.0000 8.6761 9 3371 8.6478 8.3283 6.8399 7.6974 7 9294 8@8109 .8043 .1302 .8521 .7198 .7231 .6911 .8073 .8527 .7094 .7197 .1223 .8144 .7526 .7858 .8079 .7493 .7467 .8022 @7740 .$SSG .7508 1.7501 1 7102 1.4304 1.8104 1 $$to 1,6153 1.6S*5 l@6872 CONTINUED ON NEXT PAGE -- HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. APPENDIX D INDIVIDUAL ORGAN-TO-BRAIN WEIGHT 22 WEEK SACRIFICE RATIOS --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-U;SUIRSET-ALL Bft - elAIN Ll - LIVER, LU - LUNG ORGAN ABBREVIATION: TEO - LF. fiESTIS. TEL - PT. TESTIS. SO - ACCESS/SEX ORO SEX DOSE ANIMAL TERMINAL GROUP HUM13ER BODY WT (91 - - O-R - - - - -Li- --- - - -LU - - - - -TE-O - - - - T-EI- - - - -So- - - - - - - - - - ----------------------------------------------------------------------------------------------------------- m 2 c6ls43 m 2 C67SAS 546.2 560.7 1.0000 1.0000 7@egoo 1.0803 .7120 .6107 .6192 .7107 '6140 .7666 1.0133 1.6026 NUMBER IN GROUP: MEAN@ STANDARD DEV: - - -10- - - - -10- - - - - -to- - - - -10- - - - - -10 - - - - -10- - - - - -to- - - - - - - - - - SOT.L L.0000 8.2047 @7751 @7$15 .7830 l@6271 58.1 DOOO .7112 .004S .0680 .0746 .2294 -------------w ------------------------------------------------------------------------------------------------ 3 C07561 m 3 C67567 N 3 c6?s72 m 3 C67573 m 3 C6?575 3 C67s79 * 3 CG?Sgl * 3 CG?Slg * 3 C67523 * 3 C67609 612.1 640.3 SS3.3 526.7 729.2 587.0 SIL.7 630.1 709.S 730.9 1.0000 1.0000 1.0000 1.0000 1.0000 1,0000 1.0000 1.0000 1.0000 1.0000 8.4819 1.1110 1.2713 6.5128 9.7802 8.5788 I.t423 9.9660 9@9646 8.0018 .7ls4 .1172 .7333 .7118 .7597 .7227 .9773 .757S .6732 .5119 .765S @7410 .7957 .1938 .8193 .6804 .92S2 .9076 .7770 .7554 .7791 @7034 .7934 .1986 .8281 .7106 .9693 .8975 .8270 1.1197 1.4104 l@4841 1.1074 1.5231 1.7846 1,0321 1.5536 1.7082 1.43S$ NU-MB-ER- -IN -OR-OU-P: - - - - -to- - - - -10- - - - - -10 -. - - - -10- - - - - -10 - - - - -10- - - - - -10 - - - - - - - - - - MEAN: 629.2 L.0000 8.5888 .7026 .7903 .1161 1.6320 STANDARD OEV: 73.4 .0000 I.OS99 .083S .1042 .0152 .1649 --------------w ---------------------------------------------------------------------------------------- m 4 C#1622 m 4 C67620 m 4 CS?628 563.6 S60.2 666.5 t@0000 1.0000 1.0000 10.1161 9.0171 9.6974 .7230 .7919 .7962 .8801 .9096 .8421 .9503 .9183 .8432 1 1401 2.0108 2.013S CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA, MADISON. WISCONSIN U.S.A. INC. APPENDIX D INDIVIDUAL ORGAN-YO-BRAIN WEIGHT 22 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-U;SUBSET-ALL ORGAN ABBREVIATION: 13R - BRAIN Ll LIVER, LU - LUNG TEO - LF. FESTIS,- TEI - AT. TESTIS, SO - ACCESS/SEX ORO SIX DOSE ANIMAL TERMINAL - -- -- -- -- --- - -- -- -- - -- -- - -- -- -- -- -- -- -- - GROUP HUMBER BODY WT 19) SR Ll LU TEO TEI so ---------------------------------------------------------------------------------------------------------------- m 4 t4 4 m 4 m 4 m 4 m 4 fA 4 C67637 C67049 C67651 C67660 C67S64 C6?666 C57670 520.9 001.5 614.9 5s7.8 624,3 009.1 620.S 1.0000 1.0000 1.0000 1,0000 1.0000 1.0000 1.0000 lo.ssoo 1.1761 9.7034 8.6908 9.S272 8.7206 10.865S .7881 .7090 .7512 .7264 920S .7306 .9100 .7331 .5725 .84'17 .7302 .7553 .6621 .1103 .7492 .9040 .82*5 .7495 .7709 .6546 .860S l.s6o4 1.9091 1.5208 1.6015 1.9914 1.8034 1.8297 - - - - - - - - - - - - - - - - - - - - - - - - - - - - --- - - - - - - - - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: 10 10 10 10 10 10 10 MEAN: 601.8 1.0000 9.5071 .7703 .7828 .8229 1.8158 STANDARD DEV. 51.2 .0000 .8621 .0611 .1097 .0917 .1973 ---------------------------------------------------------------------------------------------------------------- m 5 C67674 m 5 CG?676 m s C67678 m s C67683 m 5 C67684 m s C67694 m 5 C67701 m s C67704 655.4 399.4 552.5 593.0 490.9 595.7 541.1 S97.4 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 0.7543 7.1931 9.44is 8.1365 6,1434 8.7881 9.4068 9.9561 .7625 .72$S .6829 .7183 .7021 .7788 .8273 .7622 .0862 .786S .1366 .7216 .7991 .8079 .1579 .8032 .1604 .7sts .8332 .7332 .1275 .7510 IS30 .83se 1.7649 2.0640 1.7461 1.6960 1.1962 1.80$S 2.37S2 1.5438 CONTINUED ON NEXT PAGE HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. APPENDIX 0 INDIVIDUAL ORGAN-TO-BRAIN WEIGHT 22 WEEK SACRIFICE RATIOS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX-ALL;GROUP-ALL.WEEKS-ALL DEATH-U;SUBSET*ALL IBA - BRAIN Lt - LIVER. LU - LUNG ORGAN A13OREVIATION: TEO - Lf. TESTIS. TEL - NT. TESTIS. SO - ACCESS/SEX 000 SEX DOSE ANIMAL TERMINAL GROUP NUMBER BODY WY 191 - - 6-0 - - - - -Li- - - - - L-U - - - - -TE-O - - - - T-EL- - - - -So- - - - - - - - - - ---------------------------------------------------------------------------------------------------------------- 5 C67706 m 5 C6??23 S72.9 561.3 1.0000 1.0000 2.9796 7.8910 .7184 .7774 .7831 .9531 .7692 .9097 l.s7t3 1.5328 NU-MB-ER - -IN -GR-OU-P: - - - - -10- - - - -10- - - - - -10 - - - - -10- - - - - -10 - - - - -to- - - - - -10 - - - - - - - - - - - 14EAKi 556.0 L.0000 8.7300 .74SI .1242 .1140 L.1299 STANDARD DEV: 69.8 .0000 1.1471 .0434 .0027 OS63 .3198 ------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANit4AL HUMBER@ C614SL SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. FIRST INTERIM DATE Of DEATH: 01/2s/gl STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: 4 DATE AND TIME Of t4ECROPSY: 01/25/91 9:11 PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY DE ----------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R ORGAN NAME ---------------------- (GRANSI -------- TO BODY WEIGHT -------- WEI-G-HT---R-AT-I-O ST ---- BRAIN (BRI 2.2017 .4919 % 1.0000 WEIG LIVER ILI) 17.2031 3.8570 % 7.8411 WEI( LUNG (LUI 1.7496 .3909 % .7947 WEIG LF EST[ TEO) 1.7109 .3979 % .8080 WEI( ItT: TTESTISS TELI 1.7S40 .3910 z .7967 WEIG ACCESS/SEX OIG (SO) 2.6091 S$29 % 1.1850 WEI( ---------------------------------------------------------------------------------------------------------------- 0a A PA TH0 L0 0 Y 0 0 S ER VA 710 NS * 0 A O-R-GA-N---NA-M-E -------------K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---D-IS-T-R-I1-3-UT-I-O-N ---------G-R-O-SS---FR-E-E--T-E-X-T --C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-HO-L-O-GI-C--------- GENERAL COMMENT JOC GROSI: -ALL itiS6Ei/6ft6A'NS*Ni)ltit.AL. . . . . . . . . . ... . . - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATIOII: B,RAIN.IB( RI. COAGULATING GLO (CGI. LF. TESTIS (TEOI. LIVER ILIJ, LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE -------U--E-T-H----u-p-)----------------------------------------------------------------------------------------------- to 4-" INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C07452 SEX: MALE DOSE GROUP: I SACRIFICE S7A7US: SCHEDULED. FIRST POST-RE DATE OF DEATH: STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL 13ODY WEIGHT: 5 DATE AND TIME OFo5N/EjC7R/O9P1SY: OS 17/91 8:21 PROSECION: DEBBIE PINKIL RECORDER: JOE N. KIZZ POST-FIX WEIGHER: NOT AVAILAS(E PATHOLOGIST: DR. TOM PALMER WEIGHER: DENNIS HOFF ---------------------------------------------------------------------------------------------------------------- A13SOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0n ORGAN NAME ---------------------- IGRAMS) -------- 70 BODY WEIGHT 1%) -------- WEIGHT RA710 -------- sI ---- BRAIN 1L8li 2.1855 3869 % 1.0000 WEIO LIVER 18.03st 3.1027 X 8.2S2S WEIG LUNG ILUI I.SISO .2859 % .7390 WEIG LF TES is E 1.6436 .2910 % .7S20 WEIG ItT@ TES'TIS ITTE?L 1.7409 .3092 % .7993 WEIG ACCESS/SEX 0 0 ISO) ---------------------------------- 4.3278 .7661 % 1.9102 WEIG --- - - - - -- - -------- -.------- - - - --S----;-;-;----------------- P A T N 0 L 0..0 Y 0 0 S E 0 V A t 1 0 N O-R-GA-N---NA-M-E-------------K--E-Y-W-OR-D-S-. --S-E-VE-R-I-TY-,---D-IS-T-R-IB-U-T-IO-N-----------O-R-O-SS---FR-E-E--I-E-X-T--C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-HO-L-O-GI-C--------- GENERALCOMMENT 04(OICSI-:ALC iliS6Ei/6RdAiS'N6RkAE - -- ----- -- ---- ---- - ---- --- -- --THE FOLLOWING TISSUES WERE UNNE04ARKABLE AT MICROSCOPIC EXAMINATION: B:Alg,lBRI. COAGULATING OLD ICO), LF. TESTIS IYEO), LIVER (LI). LUNG ILU). PROSTATE IPP), SEMINAL VESICLE -------U--E-T ----J-U-R-)------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. 14ADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- A14lt4AL NUMBER @ C674S3 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. FIRST POST- DATE OF DEATH: OS/16/91 STUDY DAY OF DEATH: 141 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: DATE AND Ilt4E Of NECROPSY: OS/16/91 7@27 PROSECTON: DEBBIE PINKEL RECORDER: JOE N@ KI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: JACK SERFO ------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 OR-G-AN---N-AM-E-------------- JORAMS) -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- s BLRIAVIENR 2.163:3 23. 4 4 .3209 % 3.5489 % 1.0000 WE ii.osps WE LUNG ILU) 1. IkSS3 .2714 % .8677 WE LF. TESTIS TEO 1.9776 .2968 % .9248 WE AT. TESTIS TEI 2.0904 .3137 % .0776 WE ------A-C-C-ES-S-/-S-EX---O-i-a--I-S-O-)--------------3.-5-8-6-6-------------------------S3-8-3--%--------------1-.-67-7-3------------W-E--------- . * 0 a P A 7 " 0 L 0.0 Y 0 8 S E P V A T 1 0 N S A A A OVIOAN -N-A-ME--------------K-E-Y-WO-R-D-S.---S-EV-E-R-IT-Y-.---DI-S-T-RI-B-U-TI-O-N----------G--R-O--S-S---FR-E-E---TE-X-T---CO-M-E-NT-S----/---H-I-ST-O-P-AT-"-O-L!-O-G--I---- GENERAL LIVER COMMENT ILI) 0100CSI: -ALi iliS6Ei/6R6AiS*NbRf@A@ . . . . .I. .I . .. .. . .. .. .. . .. MICRO: -SLIGHT. FOCAL -MiCiOili.6OiG6LATiVi .. .. .. . - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - BRAIN 161t).COAGULATING OLD ICGI. LF. TESTIS ITEO). LUNG ILUI. PROSTATE (PR). SEMINAL VESICLE (SVI, URE --------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. HADISO", WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C674S4 SIX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. TERMINAL SAC DATE OF DEATH: 03/26/91 STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TERMINAL 13ODY WEIGHT: DATE AND TIME Of NECROPSY: 03 26/91 0:22 PROSECTON: STEVE VAN ADESTINE RECORDER@ JILL BEVA POST-FIX WEIGHER: NOT AVAILAO(E PATHOLO(IIST: ON. TOM PALMER WEIGHER: JOE N, KIZ --------------------------------------------------------------------------------------------------------------- ABSOLUTE OROAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN MAHE (GRAMSI TO BODY WEIGHT 4%1 WEIGHT RATIO s ---------------------- -------- -------- -------- BRAIN (OR) 2.1122 .3130 % 1.0000 WE LIVER ILI) 20.7989 3.GSOO % O.S312 WE LUNG Lul 1.6464 .2190 % .754S WE LF. TEITI (TEOISOI 1.730S .3038 % .1930 WE RT. TESTIS (TEL 1.7609 .309t % .0069 WE ACCESS/SEX 090 3.5431 .6219 % 1.6236 WE ---------------------------------------------------------------------------------------------------------------- I ob a A. P A T H 0 L 0.0 Y 0 0 S E 0 V A 7 1 0 N S 0 4 A OROAN NAME KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC -------------------------------------------------------------------------------------------------------- GENERAL C0144ENT G(AGOCSI:-Ali il@S6Ei/6RdAiS'N6Ri4Ai . . . ... . . - -THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- 14-1CRO-SCO-PIC- EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN (80(1,,COAGULATING GLO (CGI. Lf. TESTIS (TEO). LIVER (LI). LUNG (LUI. PROSTATE IPRI. SE14THAL VESTCL URETHRA U -------- ------------------------------------------------------------------------------------------------------ co INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C674SS SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SC14EDULED SECOND INTERIM DATE Of DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK Of DEATH: 9 TERMINAL 6ODY WEIGHT: 4 DATE AND TIME OF NECROPSY: 02/14/91 7:45 PROSECTOR: KEVIN BILLINGS RECORDER: JILL BEVAN POST-FIX WEIOHER: NOT AVAILABLE PATHOLOOIST: DR. TOM PALMER WEIGHER: VAL QUINN ----------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIOHT ORGAN NAME ---------------------- JORANS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- GROAN TO BRAIN WEIGHT RATIO -------- 0R ST ---- BLRIAVTER a 1,9707 .4269 % 1.0000 WEIG " iLlItl IS.5440 3.3522 % 7.8517 WEIG LUNG (LUI I'ssss .3s?o % .1362 WEIG LF TESTIS TEO 1.5477 .3338 % .7118 WEIG RT@ TESTTS TEI l@S$84 .3425 % .8023 WEIG ACCESS/SEX OIG lsol 2.7$IS .5955 % 1.3949 WEIG ----------------------------------------------------------------------------------------------------------------- A e a P A T N 0 L 0.0 Y 0 0 S E R V A t 1 0 N S 11& A ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPAT"OLOGIC ---------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LIJ GAOaCsi:-ALi iliS6Ei/6R6AiS'Ni)ltl@Ai . .. . .. . .. ... .. * 'INhFIiLTRtATAESi.MO*NO6NTI6CL'EAiR A 'CELL THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN (SRI, COAGULATING OLD ICO), LF. TESTIS (TIOI, LUNG ILUI. PROSTATE IPRI, SEMINAL VESICLE ISVI. URETH ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- A14INAL NUMBER: C674SG SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST POST-N DATE OF DEATH: OS/16/91 STUDY DAY OF DEATH: 141 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: DS 18/91 7:41 PROSECTOlt: DEBBIE PIRKEL RECORDER: JOE N. KIZ POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER: JACK SERFOR ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 O-R-GA-N---NA-M-E -------------- JOITANS) TO BODY WEIGHT WEIGHT RA710 s7 -------- -------- -------- BRIAINit 1L84li L VE LUNG ILU) Lf TESTIS ItT: TESTIS 17YEE?i 2.14so 17.3131 l.s932 1.4los 1.4101 3797 % 3:0772 1 .2120 % .2497 % .2490 1 1.0000 WEI 1.1040 WEI .7421 WEI .6576 WEI .0574 WEI ACCESS/SEX 0 0 ISO) 3.S6S8 .0312 Z 1.6624 WEI ---------------------------------------------------------------------------------------------------------------- a a I. P A I H 0 L 0-0 Y 0 8 S E R V A I 1 0 N S & A & ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC --------------------------------------------------------------------------------------------------------- GENERAL COMMENT c al(ols-tA:Li iliS&Ei/6lt6AiS*t461tkA@ THE- FO-LLOW-lt4-G -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COPIC EXAMINATIONBURRAIN OR) COAGULATING GLD ICO). LF. IESIIS IIEOI. LIVER ILI). LUNG ILU). PROSTATE IPR), SEMINAL VESICL ETHRI (Uil -----------------------------------------------------------------------------------j---------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ----------------------; ------------------------------------------------------------------------------------------ ANIMAL NUMBER: C$7457 DATE Of DEATH: 01/24/91 SEX: MALE DOSE GROUP: I STUDY DAY OF DEATH: 36 SACRIFICE STATUS: SCHEDULED. FIRST INTERIM STUDY WEEK OF DEATH: 0 TERMINAL BODY WEIOHT: DATE AND TIME OF NECROPSY: (24/91 8:38 POST-FIX WEIGHER: NOT AVAILAoSl E PROSECTON: KEVIN 13ILLINGS PATHOLOGIST: DR. TOM PALMER RECORDER: VAL QUINN --------------------------------------------------------------------------------W-E-IG-H-E-R:---D-E-BB-I-E--P-I-N-K --------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 ---------------------BRAIN (BRI -------2-0693 .3087 -------ssol % WEIGHT RATIO -------1.0000 ST --WEI( LIVER (LI) LUNG (LUP 15 1.4270 4.0747 % .3791 % 7.3980 WEI( LF TESTIS TE RT: TESTIS TE? l.s37o 1.5080 .4091 x .0890 WEIG .7421 WEIO ACCESS/SEX 010 ISO) 2.67s4 .4014 % .7121 % .7217 WEIG 1.2929 -----------------------------------------------------------------------------------------------W-E-IG---------------- ORGAN NAME PA TN0 L0 0 Y 00 S ER VA T10 NS 01 0 KEYWORDS, S0EVmER*ITY. DISTRIBUTION GE-NE-R-A-L --C-O-MM-E-N-T ----------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-H-OL-O-G-IC--------- GI(OICSI-:ALi iliS6Ei/6R6AhS*N6ltt@Ai . . ... . . THE- FO-LLOW-ING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - UBRREAITNHRIBR(IUi)COAGULATING OLD (CGI. LF. TESTIS ITEO). LIVER ILI). LUNG (LU). PROSTATE IPR SEM INAL VESICLE ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES . AMERICA. INC. MADISON. WISCONSIN U S.A. ---------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C67458 DATE OF DEATH: 02/14/91 SEX: MALE DOSE GROUP: i STUDY DAY OF DEATH; SY SACRIFICE STATUS: SCHEDULED. SECOND INTERI STUDY WEEK OF DEATH: 9 DATE AND TIME OF NECROPSY: 02/14/91 1:32 PROSECTOR: PUT" SCHMITZ TERMINAL BODY WEIGHT: POST-Fix WEIOIIER: NOT AVAILABLE PATIFOLOGIST; DR. TOM PALMER RECORDER: JILL BEVAN ------------------------------------------------------------------------------WE-I-G-HE-R-:---V-AL--Q-U-IN-N----------------- ORGAN NAME ABSOLUTE OROAN WEIO"T (GRAMS) 070AN WEIGHT RELATIVE OROAN TO BRAIN 0 ---------------------BRAIN 0 -------- 0 BODY WEIO"T IZI -------- WEIO"T RATIO -------- ST --- LIVER IL*ll 2.0532 16.0617 .4554 2 1.0000 WEI LUNG LF. TEITLIUI f TE (TE0II 1.5344 1.6309 3.5021 % .3403 X .3030 2 7.8221 WEI .7473 WEI .7972 WEI NT. TESTIS ACCESS/SEX ORO Sol 1.5900 2.5526 .3520 % .7744 WEI -------------------------------------------------------------------.-5-0-6-1 -%--------------1-.-2-4-32-------------WE-I-------- ORGAN NAME -------------- --GENERAL COMMENT (ac a aa PA T "0 L0 0V KEYWORDS. SEVERITY. DISTRIBUTION 0 8 S E ItV A T 1 0 N S * a e GROSS FREE-TEXT COMMENTS / "ISTOPATHOLO(ITC -------------------------------------------- anosi; -ALi iliS6Ei/6#6AiS'N61ti4Ai . . . . . . . . . . . . . . . . ... . . T HE FOLLOWI BRAIN NO TISSUES WEItE-UNftEMARKABLE A T 141Clt0 SCOPIC EXAMINATI 0 m: - - - - - - - - --- -- u Bit) COAGULATING GLD I lual ICO). LF. TESTIS (TEO). LIVER (Lll. LUNG (LU). PROSTATE (Pot). SEMINAL VESICLE ----------------------------------------------------------------------------------------------------------------- C3 r..) INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES A14ERICA, INC@ MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C674S9 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED, TERMINAL SAC DATE OF DEATH: 03126191 STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIO"T: DATE AND TIME OF NECITOPSY: 03/26/91 9:10 PROSECTON: DEBBIE FINKEL RECORDER: JILL SEVA P-O-S-T--F-I-X --W-EI-G-H-ER-:---N-O-T -A-V-A-IL-A-1-3L-E----------------P-AT-H-O-LO-G-I-ST-:---D-O-. --T-OM--P-A-LM-E-R----------------W-E-I-GH-E-R-:--J-O-E----M-.---K--I--Z------ ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- s BRAIN JORI 2.1392 .43is % 1.0000 WE LIVER Ll ls.7049 3.1616 % 7.3415 WE LUNG (LUI 1.5298 .3086 % .7151 WE LF TESTIS TEO 1.8054 .3641 % .8440 WE RT.* TESTIS tEt 1,8218 .3614 % WE ACCESS/SEX 010 ISO)-------------2-.-9-0-89-------------------.-5-8-67--%--------------1-.-3-59-8-------------WE------------ a s a P A T H 0 L0 0 Y 0 8 S E N V A T 1 0 N S I * 0 ORGAN NAME KEYWORDS, SEVERITY DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGI -----------------------------------:----------------------------------------------------------------- GENERAL GENERAL COMMENT OIGOCSI : -ALi iliS&Ei/bit6AAS'N61tkAi INFORMATION (XXI . . . . . . . . . . GROSS: NECROpsi hoie: *)H,AS'SIVE*N,TR*AL*H,IND,*RI,GH*Tl,N*OTE,D,I*NC*L,IN,IC,AL FOUND AT NECROPSY - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - BRAI,N,(87),,COAGULATING OLD (COI. LF. TESTIS ITEO). LIVER (LI), LUNG (LUP, PROSTATE IPP). SEMINAL VESIC -------U-R-E-I---A--U------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NU14BER: C67460 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED TERMINAL SACO DATE OF DEATH: 03/2S/91 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: DATE AMD TIME OF NECROPSV@ 03 2S/21 7:31 PROSECTON: JULIE A. LARSEN RECOROER@ JOE M. KIZ POST-FIX WEIGHER: NOT AVATEAS(E PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN ------------------------------------------------------------------------------------------------------------ ORGAN NAME --------------------- ABSOLUTE ORGAN WEIOHT (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 ST --- OIAIMIt 1L8li L VE LUNG JLUI LF TESTIS RT@ TESTIS TEO11 if 2.414t 20.9803 1.6769 1.6212 I.GBSO ds3o % 3.0455 % .3LS2 % .3048 x .3169 % 1.0000 WEI 1.6932 WEI .6946 WEI .6716 WEI .6910 WEI ACCESS/SEX ------- --O-I-G---(-S-O)---------------4-.-1-4-41--------------------.7-7-9-2 -X--------------1-.-7-1-69---- ----------WE-I--- . a ;; PA TH 0 L00 Y 0 8 SfN VA T10 NS ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS HISTOPATHOLOGIC -------------------------------------------------------------------------------------------------------- GENERAL COMMENT GI(OICSI:-ALL iliS6Ei/61tdAiS'ti6RitAC LIVER (Lil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -MINIMAL. FOCAL INFILTRATES.MONONUCLEAR CELL THE FOLLOWING TISSUES WERE UNREt4ARKABLE AT MICROSCOPIC EXAMtHATION7 BRAIN ION), COAGULATING GLO (CGI. LF. TESTIS (TEOI. LUNG (LU), PROSTATE IPAI. SEMINAL VESICLE (SVI, URET --------------------------------------------------------------------------------------------------------------- ob INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, MADISON. WISCONSIN U.S.A. INC. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C87461 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST INTERI DATE OF DEATH: 01/23/91 STUDY DAY OF DEATH: 35 STUDY WEEK OF DEATH: 5 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 01/23/91 8:34 PROSECTON: JULIE A. LARSEN RECORDER: JILL SEVA POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: OR. TOM PALMER WEIGHER: GAIL M. NO --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMSI TO BODY WEIGHT (%I WEIGHT RATIO s ---------------------- -------- -------- -------- BRAIN LIVE LUNO ICONI 1 (LU) 2.1204 16.3219 1.5015 5114 % 3.9907 % .3611 % 1.0000 WE 7.6976 WE .7081 WE LF. TESTIIS OIIEOIISOI 1.57$S .38SO % .1444 WE PT. TEST S TE 1.649S .4033 % .?779 WE ACCESS/SEX (1 2.1661 .7001 % 1.3sl7 WE --------------------------------------------------------------------------------------------------------------- a a It P A T " 0 L 0 0 Y 0 9 S E R V A T 1 0 N S 0 * A ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTICIN GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI ---------------------- ---------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LI) GAGOCSI: -iLi iliS6Ei/6R6AhS*NbRi4AL M16R6:'-iLiQAT* eO@Ai .. .. .. - ---- ----- --- ---- ---- -- --- ----- -- --- - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN IBRI. COAGULATING OLD ICOI. LF. TESTIS (TEO). LUNO (LU), PROSTATE (PRI, SEMINAL ...,;ICLE(SVI. URE -------- ------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. MADISON. WISCONSIN U.S.A. INC. ---------------------------------------------------------------------------------------------------------------- ANIMAL NU14BER: C$7402 DATE OF DEATH: 03/25/91 SIX: 14ALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. TERMINAL SACR STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 DATE AND TIME OF NECROPSY: 03(25/91 9:50 PROSECTOR: JULIE A. LARSEN TERMINAL BODY WEIOHT: RECORDER: JOE N. KIZ P-O-S-T--F-I-X---W-EI-G-H-ER-:---N-O-T--A-VA-I-L-A-O --E------------------PA-T-H-OL-O-G-IS-T-:----DR-.---TO-M--P-A-L-ME-R------------------W-E-IG-H-E-R:----S-TE-V-E--V-A-N--A------- ORGAN NAME ABSOLUTE (GROARMGSA)N WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- s7 --- BRAIN LIVER 2.1057 .3529 % 1.0000 WEI LUNG (LUI 11.3191 1.4899 3.0011 X .2497 % 8.7330 WEI .7076 LF TESTIS ItT@ TESTIS ITTEO11 E 1.7111 1.7431 .29$S % WEI .8451 WEI ACCESS/SEX 0 0 Sol --------------------------------- 4.2601 .2922 % .7139 % .8281 WEI 2.0231 WEI 7 ------------------------------------------------------------------------------ aa a PA T"0 L0 0 Y 0B S EN VA T 10 NS aa a ORGAN NAME ---------------------- KEYWORDS. SEVERITY, DISTRIBUTION GROSS --------------------------------------- FREE-TEXT COMMENTS / HISTOPATHOLOGIC GENERAL COMMENT ------------------------------------:------ LIVER (LI) . G.AOo.Sil.:.-A.Li. i.ti.S6.Ei./b.Ot.dA.h.S*N.6R.i.4A.i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -SLIGHT. FOCAL -INFILTRATES.MONONUCLEAlt CELL THE- FO-LLOW-ING - T-ISSU-ES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - --------B-R-A-IN---(-O-R-),--C-O-A-G-U-LA-T-I-N-G--O-L-D---(C-O-)-.--L-F-.--T-E-ST-I-S---(-TE-O-)-,--L-U-N-G--I-L-U-).---PR-O-S-T-A-TE---(-P-R-I-.--S-E-MI-N-A-L--V-E-S-I-CL-E---(-S-V-).--U-R-E-T ------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?463 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED SECOND INTERI DATE OF DEATH: 02/12/01 STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02112/91 9:30 PROSECTOR: KEVIN BILLINGS RECORDER: DENNIS "OF POST-FIX WEIGHER: NOT AVAILABLE PATHOLO(IIST: OR. TOM PALMER WEIGHER: PATHOLOGY I ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE M AN TO BRAIN 0 ORGAN NAME (GRAMS) TO BODY WEIGHT WEIGHT RATIO s ---------------------- -------- -------- -------- BIAIRM 16L0il L VE LUNG (LUI LF. TESTIS (TEO 2.2372 16.5945 1.7612 1.659S .4634 % 3.4386 X .3664 % .3439 X 1.0000 WEI 7.417S WEI .7904 WEI .?410 WEI OT. TESTIS (TEI 1.7104 .3544 % .764S WEI ACCESS/SEX 090 ISO) 3.5580 .7373 % I.S904 WEI ---------------------------------------------------------------------------------------------------------------- oba a P A T N 0 L 0 0 Y 0 8 S E R V A T 1 0 N S m b 0 ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION OPOSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC --------------------------------------------------------------------------------------------------------- GENERAL COMMENT (;I'OOCS-IA:L@ iliS6Ei/6R6AhS*N6Ri4Ai - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BURRAINHRA(ORI 1, COAGULATING OLD ICGI, Lf. TESTIS (TEOI. LIVER ILIP. LUNG (LU). PROSTATE (PRI. SEMINAL VESICL ---------E------U------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7404 DATE Of DEATH: 01/2S/91 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. FIRST INTERIM STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 0 TERMINAL BODY WEIGHT: DATE AND TIME OF UTCROPSY: 01/25/01 8:08 PROSECTOR: NANCY DIEDRIC14 RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. T014 PAL14ER WEIGHER; PATHOLOGY D ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- JORANS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 ST ... BRAIN JORI 2.0479 .4801 % 1.0000 WEI LIVER Ll 17.2sio 4.OSIO % 1.4267 WEI LYN(I LLUI I.Siol .3564 X .7413 WEI L TE Ti TEOI 1.6126 .3786 % .1874 WEI ItT: TESTIS TE 1.650S .387S % lose WEI ACCESS/SEX 010 ISO) 2.3400 .5494 % 1.1426 WEI ---------------------------------------------------------------------------------------------------------------- 0.* t P A T H 0 L 0 0 Y 0 0 S E R V A T 1 0 N S A * A ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC ----------------------------------------------------------------------------------------------7 ------ GENERAL COM14ENT LIVER (LI) 04(OICSI: -ALi iliS&Ei/6lt6AhS*N6Ri4Ai .. .. .. .. .. MICRO: -MINIMAL. F6CAL' -liFiLiltATiS'M6N6N6C@EAtt'CiLi -- ----- - .. .. .. - - - -- - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- BRAIN IBRI. COAGULATING OLD ICO), LF. TESTIS ITEO). LUNG ILU). PROSTATE IPRI. SEMINAL VESICLE (SVI. URET ----------------------w-------------------w-------------W------------------------------------------------- 40 INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7465 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. SECDI#D INTERI DATE Of DEAIH:0?2413491 STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL BODY WEIGHT: DATE AND TIME EC OPSY: 02 13/91 9:04 PROSECTOR: DENNIS tffFt4AN RECORDER: KEVIN BILL POST-FIX WEIGHER: NOT AVAILAB(E PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ------------------- JORAMS) -------- 70 BODY WEIGHT 1%) -------- WEIGHT RATIO -------- sT --- BIAIR" 1L0li 2.1366 S062 1 1.0000 WEI LV ls.6409 3:7113 % 7.3439 WEI LUNG ILUI 1.4707 .3414 % .8883 WEI LIRFT@ TEESSTTIISS TEOI 1.1221 .4317 % .8528 WEI TEIISOI 1.70SO .4113 % .8264 WEI ACCESS/SEX JO 2.S4$4 .0037 % 1.1927 WEI --------------------------------------------------------------------------------------------------------------- . A It 0 P A I H 0 L 0--(iY 0 0 S E N V A T 1 0 N S * A * O-R-GA-N---NA-M-E-------------K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-,---DI-S-T-RI-B-U-TI-O-N----------G--R-O--S-S---FR-E-E--T-E-X-T --C-O-M-14-E-N-TS---/---HI-S-T-OP-A-T-HO-L-OG-I-C-------- OINERAL COMMENT 04(olCsl-:ALL' iliS6lEi/6R6AiS'N6Ri4Ai . . . . - -THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNN-E04A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - BRAIN"Itl"I R1, COAGULATING OLD ICO). LF. IES71S 17EO). LIVER ILI). LUNG ILU). PROSTATE 1PIti,SEMI"AL VESICL -------U-RE-T -----u------------------------------------------------------------------------------------------------ APPENDIX D INDIVIDUAL AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: CO?466 SIX: KALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. SECOND INTENT DATE OF DEATH: 02/13/91 STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL BODY WEIOHT: DATE AND T114E OF NECROPSY: 02 13/01 I:OS PROSECTOR: JULIE A. LARSEN RECORDER: KEVIN BILL POST-FIX WEIO"ER: NOT AVAILAIB(E PATHOLOGIST: DR. TOM PALMER WEIOHER: JILL BEVAN ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WIEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (ORANSI -------- TO BODY WEIGHT 1%1 -------- WEIGHT RATIO -------- ST --- BRIAINR 1L80li 2@0132 .4249 % 1.0000 WEI L VE 19.3228 1.4311 3.9410 % .2919 % 9.27SS WEI .0870 WEI LUNG (LU) LF TESTIS ITEOISOI 1.5484 .3158 % .7432 WEI RT* TESTILrS OITEI 1.6171 .3300 % .7760 WEI AC6ESS/S X 0 3,1714 .6483 % I.S2S? WEI ---------------------------------------------------------------------------------------------------------------- . & 11a P A I N 0 L 0 0 Y 0 0 S E R V A I 1 0 N S A s A O-R-GA-N--N-A-M-E -------------K--E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---D-IS-T-R-IB-U-T-IO-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-"O-L-O-GI-C-------- GENERAL COM14ENT (11'OCSI-:ALi iliS6Ei/6R6AkS'N61tkA@ . . . . . * * * . . . . . . . . - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMII-IAT-TON:- - - - - - - - - - - - - - lURlElTtHlRIlI"IIT1U,,COAGULATING OLD (CGI. LF, TESTIS (TEO). LIVER ILIJ. LUNG ILU), PROSTATE IPRI, SEMINAL VESICL --------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67467 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST INTERIM DATE OF DEATH: 01/2S/91 STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH-. 6 TERMINAL IODY WEIGHT: DATE AND TIME OF NECROPSY: 01 25/91 0:40 PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PAL14EN WEIGHER: PAT14OLOGY D ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO GRAIN 0 ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- ST ... BIAIRN 1L0li 2.1917 S220 % 1.0000 WEI L VE 14,6861 1,6460 3.492S % .3914 % 6.612S WEI .7490 WEI LUNG ILU) Lf. TESTIS 17E I.S$41 .3161 % .7208 WEI RT. TESTIS IT[0il 1.5651 .3722 % .7122 WEI -------AC-C-ES-S-/-SE-X---O-RO----S-O-)--------------2.-1-6-9-0-------------------.6-8-2-3--% ---------------------1--.-3--0-55-------------W-EI----- @ ola & P A I " 0 L 0 0 V 0 8 S E P V A T 1 0 N S A a A O-R-O-AN---NA-M-E -------------K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R-O--S-S---FR-E-E--T-E-X-T --C-O-MM-E-N-TS----/---"I-S-T-OP-A-T-HO-L-O-GI-C---- GENERAL COMMENT GI(OICSI-:iLi iliS6Ei/6R6AiS*N6Ri4Ai .. ... . - -THE- FO-LLO-WINO- -TISS-UES- WE-RE -UNR-E14A-RKA-BLE - Al- MI-CROS-COP-IC - EX-A141-HAT-ION:- - - - - - - - - - - - - - BRAIN,,11I3RI COAGULATING OLD (CO), LF. TESTIS (TEO). LIVER (LI), LUNG (LU). PROSTATE (PRI. SEMINAL VESICL -------U-,T-ET-H-----U*-)---------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67468 DATE OF DEATH: Ot423/91 SEX: t4ALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST INTERIM STUDY DAY OF DEATH: 2S STUDY WEEK OF DEATH: 5 TERMINAL 6ODY WEIGHT: 3 DATE AND TIME OF ECROPSY: 01/23/91 8:43 PROSECTOR: NANCY DIEDRICH RECORDER: JILL BEVAN PosT-rix WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: GAIL M. NOVA ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R ORGAN NAME ---------------------- JORAMS) -------- To BODY WEIGHT -------- WEIGHT RA710 -------- sI ---- BRAIN (OR) 2.0288 .5097 % 1.0000 WEIG L (LIJ 14.9008 3.7439 % 7.3446 WEIO LUNG LUI (TEO, 1.584S 1.7321 .3911 % .4352 % .7810 WEIG .8538 WEIG LFIVE:ELTI RT, TESTIS ITELI 1.8084 .4544 % .8914 WEIG ------A-C-C-ES-S-/S-E-X---0-0-0---(-SO-)-----@---7 -------2-.-S-$-29--------------------.6-4-9-0--% --------------1-.-21-3-1------------W-E-IG------------ a e Ob P A T " 0 L 0-0 Y 0 0 S E R V A T 1 0 N S A * * O-R-GA-N---NA-M-E -------------K--E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S--S --F-RE-E---7E-X-7--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-IC--------- GENERAL COMMENT AGCI G OSS: -A.LL. T.IS.S 6Ei/606AiS'N6RilAi LIVER (LIJ t4l6R6:'-i4l4ll4Ai.'F6CA*L' * * ' * * ' ' * ' ' :IhFiLiltiTiS*M6N6N6CiEAR'CiLL - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN (BR), COAGULATING GLD ICO). Lr, TESTIS ITEO). LUNG ILU). PROS7A7E IPRI, SEMINAL VESICLE (SV), URETT ----------------------------------------------------------------------------------------------------------------- APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7469 DATE Of DEATH: 02/13/91 SEX: 14ALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED SECOND INTERI STUDY DAY OF DEATH: S6 DATE AND TIME OF NECROPSY: 02(13/91 9:17 STLN)Y WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILAO E PROSECTOR: RUTH SCHMITZ RECORDER: KEVIN BILL ------------------------------------------P-A-TH-O-L-OG-I-S-T:---D-R-.--T-OM--P-A-L-ME-R----------------W-E-IG-H-E-R-: --J-I-L-L --BE-V-A-N---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- ST BRAIN LIVER 2.0755 21.344S .4201 % 4.3207 % 1.0000 10.2840 --- WEI WEI LUNG ILU) LF TESTIS 1.5986 1.6613 .3230 % .3303 % .7702 WEI RT@ TESTIS ITTEE101 ACCESS/SEX 0 0 Sol 1 7176 3.0237 .2477 % .8004 .8276 WEI WEI( ------------------------------------------------------------.-6-12-1--i-t-------------1-.-4s-o-o------------W-E-I-(--------------- ORGAN NAME PA TH0 L0 0 Y 0 8 SE RV A T 10N S KEYWORDS. S*EV'ER4ITY, DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENT; -/a HISTOPATHOLOOIC OEMERAL COMMENT --------------------------------------------- LIVER ILI) 0100CSI;-ALi iliS6Ei/i)ftdAAS*N6RilA@ . . . . . . . MldR6: '-i4lilkC. -INFILTRATES.MONONUCLEAR CE.LL . . . . . . THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - BRAIN (ON). COAGULATING OLD (CO). LF. TESTIS (TEO), LUNG (LUI. PROSTATE (PRI, SEMINAL VESICLE (SVI. URET" ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?470 SEX: 14ALE DOSE GROUP: I DATE OF DEATH: 03/26 91 STUDY DAY OF DEATH: 97 DATE AND TIME Of NEC40PSY: 03(26/01 S:SS SACRIFICE STATUS: SCHEDULED TERMINAL SACRI STUDY WEEK OF DEATH: 14 TERMINAL 6ODY Wfl()HT: S POST-FIX WEIGHER: NOT AVAILA13 E PROSECTOR: STEVE VAN ADESTINE PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-IG-H-E-R:---J-O-E --N.--K-T-ZZ-I---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R ---------------------- -------- TO BODY WEIGHT (z) -------- WEIGHT RATIO -------- ST BRAIN (13R) LIVER ILI) 2.0425 .3930 % 1.0000 ---- ILUI 20.1604 I.Siol 3.8104 % WEIG 9.8734 WEIG LLUFN.GE TE TI TEO1 1.8097 .2921 % .7433 WEIO RY. TESTIS TE ACC SS/SEX io lsol 1.7907 .3412 % .3440 % .8160 .8767 WEIGI WEIOF -------------------------------------3-.3-$-S-e-------------------.6-5-1-S--% --------------1-.6-5-7-6------------W-E-IG-H---------------- ORGAN NAME P A T " 0 L 0.43Y 0 9 S E R V A T 1 0 M S 6 a A KEYWORDS. S*EV'ER*ITY. DISTRIBUTION I O-E-ME-R-AL---C-O-MM-E-NT------c-----------------------------------------G--R-O--S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---"I-S-T-O-PA-T-H-OL-O7-G--IC-------F- GA(olsl:-ALi il@S6lEi/i)ltdAiS'N6ltkAi .. .. ... . THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNN-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BURRAIN IBRI COAGULATING (Uil OLD (CG). LF. TESTIS ITEOI. LIVER (LIP, LUNG (LU), PROSTATE (PRI. SEMINAL VESICLE ------------------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. MADISON. WISCONSIN U.S.A. INC. ---------------------------------------------------------------------------------------------------------------- ANlt4AL NUMBER: C67471 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST POST-N DATE OF DEATH: 05417191 STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: DATE AND TIME Of ECROPSY: 05 17/91 0:31 PROSECTOR: RUTH SCHMITZ RECORDER: JOE N. KIZ POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER: DENNIS HOFF ---------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE (GROARMGSAN) WEIGHT ORTGOAN BODWYIETOWNETIGHRTELAT1I%V)E ORWGEAINGHT TO RABTRIAOIN 0 ---------------------- BRAIN 0 -------2.2174 -------.3392 % -------1.0000 ST --WEI LIVER ILRIJ LUNG ILUI 23.0661 1.6203 3.S2$0 X 10.4023 WEI LF TESTIS RT@ TESTIS ITTEE011 1.7540 1.7921 .2472 % .2683 % .2741 % .7307 WEI .7910 WEI .8012 WEI ACCESS/SEX 0 G Sol 3.8614 .5906 % 1.7414 WEI ---------------------------------------------------------------------------------------------------------------- . A a i% P A I H 0 L 0 0 Y 0 8 S E N V A I 1 0 N S A A A ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION ---------------------- ---------------------------------------G-R-O-SS---F-R-EE---T-EX-T---C-O-M-ME-N-T-S----/---HI-S-T-O-PA-T-"-OL-O-O-I-C ----- GENERAL COMMENT PROSTATE IPRI GI(OICSI:-AL@ iliS6Ei/i6N6AiS'N61ti4AE . .. .. .. . MICRO: -SLIGHT. 00@A@ THE FOLLOWING ---- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATIO-N: - - - - - - - - - - - - - - - BRAIN IBRI, COAGULATING OLD (CGI. LF. TESTIS (TEO), LIVER (LIJ. LUNG ILU). SEMINAL VESICLE (SV). URETHRA ---------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C@7472 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. SECOND INTER DATE OF DEATH: 02/13/91 STUDY DAY OF DEATH: 50 DATE AND TIME OF NECROPSY: 02/13/01 7:50 STUDY WEEK OF DEATH: I TERMINAL BODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PROSECTON: JULIE A. LARSEN PATHOLOGIST: DO. TOM PALMER RECORDER: KEVIN BIL --------------------------------------------------------------------------------W-E-IG-H-E-R:---J-I-LL--B-E-V-AN----------------- OltOAN NAME ABSOLUTE (GORRAGMASN) WEIGHT ORTGOANBODWYEIGWHETIGHTRELATIVE ORGAN TO BRAIN 0 ---------------------- -------- -------- WEI-GH-T---R-AT-I-O ST --- BRAIN LIVER ILONII 2.0210 .3912 X 1.0000 WEI LUNG (LUI 21.0510 l.s394 4.0747 X .2979 % 10.41$S WEI .76is WEI LF TESTIS ItT: TESTIS j TTE0E1 1.9755 1.9$SS .3123 1 .9772 WE[ ACCESS/SEX 0 Sol 3.3SSO .3842 % .6492 % .9821 WEI l.esoo WEI ---------------------------------------------------------------------------------------------------------------- ORGAN NAME a a 0. P A T H 0 L 0'0 Y 0 9 S E P V A T 1 0 N S ita 0 KEYWORDS. SEVERITY. DISTRIBUTION ----------------------------------------------------------G--R-O--S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-IC-------- GENERAL COMMENT GI(OICSI-:ALi iliS6E@/bRdAiS'N6ltilAE ' * . . . . . . . .. . ... ** ' - -THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNN-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - - OU,,TTEATIHNRJON(U1R.I COAGULATING OLD (CO), LF. TESTIS (TEO). LIVER (LI LUNG (LU) . PROSTATE (PR 1. SEMINAL VESICL -------- ------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LA130RATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67473 DATE OF DEATH: 03/2S/91 SEX: 14ALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED, TERMINAL SACR DATE AND TIME OF NECROPSY: 03 STUDY DAY OF 25/01 1:02 DEATH- 90 PROSECTOR: STUDY WEEK OF DEATH: JULIE A. LARSEN 14 TERMINAL BODY WEIGHT: P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-OT--A-V-A-TL-A-B-(E-----------------P-AT-H-O-LO-G-IS-T-:---D-R-. --T-OM--P-ALMER RECORDER: JOE M. KIZ WEIGHER: STEVE VAN A --- ------------------------ --- ---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT IGRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 BRAIN TO BODY WEIGHT IX) -------- WEIGHT RATIO -------- ST --- LIVER ILOll 1 ::27 23 41 .3204 % 1.0000 WEI LUNG 1.5642 3.9254 % 12.0263 WE[ LF, TEI'TUtl ITE1O PT. TESTIS (TE 1,7392 .2562 % .2849 % .7850 .1728 WEI WEI( ACCESS/SEX DAG isol 1 7838 3@S24S .2922 % .89si WEI( --------------------------------------------------------------------.57-7-3--% --------------1-.7-6-8-7------------W-E-I(------- ORGAN NAME PA TN0 L0 0 Y KEYWORDS. SEVERITY. DISTRIBUTION 0 0 S E R V A I 1 0 N S a ok0 ONOSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC ;@-RD-1-0-/-C-E-R-V----L--N------------------------------------------------------------------------------------------- SRMO"IS!-iAiGi MICRO: -MODERATE, .. .. . MULTI-FOCAL . . . . . .LE.F.T:.UP. T.O.1.6 X. 1.0.X.I.MM. I.F.OR.CO.M.PA.R.I.S.ON.R. 5 X 4 X 3 MM) ICAP -HYPERPLASTA, LYMPHOOIRTICULAN CELL THE- FO-LLOW-ING TISSUES WERE-UNREMARKABLE AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BuRAIN ION()UA) COAOULATING GLD (CGI. LF. TESTIS (TEO). LIVER ILI). LUNG (LU). PROSTATE (Plt),SEMINAL VESICLE ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: CG?474 DATE OF DEATH: 02/14/91 SEX: MALE DOSE GROUP: I SAC!IFICE STATUS: SC14EDULED, SECOND INTERI STUDY DAY OF DEATH: 57 DATE AND TIME OF NECROPSY: 02 14/91 9:04 STUDY WEEK OF 0 ATH: 9 TERMINAL BODY WEIGHT: POST-FIX WEIGHER: NOT AVAILAO(E PROSECTON: DENNIS 14OFF14AN RECORDER: JILL BEV AN ------------------------------------------P-A-T-HO-L-O-GI-S-T:---D-R-.--T-O-M--PA-L-M-ER-----------------WE-I-G-HE-R-:--G-A-I-L--M-. -N-O-V--------------- ORGAN NAME ABSOLUTE ORGAN W[IONT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIOHT OftOAN TO BRAIN 0 ---------------------BRAIN (OR) LIVER ILI) -------- 2.0492 17.S$71 -------.4216 % WEIGHT RATIO -------1.0000 ST --WEI( LUNG ILU) LF. TESTIS (TE 1.5651 3.6705 % .3274 % I.S$24 .7038 WEI( WEIG RT. TESTIS ITEI0I 1.7109 1.1102 .3s9l % .3723 % .8378 WEIG -------AC-C-ES-S-/-SE-X---O-RO-----So-l--------------3-.-3-95-9--------------------7-10-3--%---- .8687 I.SS72 WEIG a lkdk - - - - - - - - - - - - -------------WE-I-G -- OROAN -NAME ------------ KEYWORDS. P A T H 0 L 0.0 Y -SEVERITY. DISTRIBUTION 09S F P VA T 1 0N S a a & GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC OINERAL COMMENT c - ------------ --------------------------------------------- (iloost:-ALi itiS6Ei/61tdAiS'N6RkAi . . . * .. .. .. .. . .. .. . .. ... .. T HE FO-LLO-WINO- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BUpRAINNot I"IItl, COAGULATING (ILD (CO). LF. TESTIS ITCOI. LIVER ILIJ. LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE ET A U I ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, 14ADISON, WISCONSIN U.S.A. INC. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67475 DATE OF DEATH: 03 22491 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED TERMINAL SACR STUDY DAY OF DEATH: 93 DATE AND TIME OF 4EC OPSY: 03/22/91 3:50 STUDY WEEK OF DEATH: 14 TERMINAL IODY WEIGHT: POST -FIX WEIGHER: NOT AVAILABLE KEVIN BILLINGS PPAROTHSEOCLOTGORI:SDTR:. TON PALMER RECORDER: JOE N. KIZ ------------------------------------------- ----- ------ --------------------------------WE-IGHER: JILL BEVAN ORGAN NAME ABSOLUTE ORGAN WEIGHT ---- OR TOGAN WEIGHT RELATIVE -------- ---- ----ORGAN TO BRAIN ---------------------- JORAMS) BODY WEIGHT 121 WEIGHT RATIO 0 BRAIN 2.3230 -------- -------- ST --- LIVER IOL'I'L 20.4091 .4094 % 1.0000 WEI LUNG LF. TEILTIUI (TIEOI 1.7021 l@9015 3.5970 % .3153 % .33SI % 8.7057 WEI .7702 WEI .1116 RT. TESTIS ITEI ACCESS/SEX ORO (Sol 1.0115 3.5652 .3379 % WE 11 .8254 WE -----------------------------------------------------------------6-2-1-3- % - -- -- -- -- - l.-s-3-4-7--------------WE-I-- ORGAN NAME P A T N 0 L 0-0 Y 0 8 S E It V A T 1 0 N S %a & ------- ------- - KEYWORDS. SEVERITY. DISTRIBUTION ---------------------------------------GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC GENERAL COMMENT c --------------------------------------- LIVER (LI) ()lost: -ALL itiS6Ei/6R6A*NS'N60t'NA.L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .NECROPSY NOTE: )@TItIMMiNd 60AMiNi:' i)NiY'OhE.PiE6E.Oe AT T114E OF TRIMMING. @l@Ei i --- --- ----- --- ----- --- THE FOLLOWINO TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION-. BURRAEITN"RiBRjIuilCOAOULATING OLD ICO). LF. TESTIS (TEO), LIVER ILI). LUNG (LU). PROSTATE (Pltl. SEMINAL VESICLE ---------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?470 SEX: MALE DOSE GROUP: I DATE OF DEATH: 01125191 STUDY DAY OF DEATH: 37 DATE AND TIME OF NECROPSY: 01 SACRIFICE STATUS: SCHEDULED. FIRST INTERIM STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: 25101 8:39 POST-FIX WEIGHER: NOT AVAILAO(E PROSECTON: DENNIS HOFFMAN RECORDER: JILL BEVAN -----------------------------------------P-A-TH-O-LO-G-I-ST-:---D-R-.--TO-M--P-A-L-ME-R----------------W-E-IG-H-ER-:---P-A-TH-O-LO-G-Y---D --------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) OR2AN TO BRAIN 0 ---------------------BRAIN -------- -------- W IGHT RATIO -------- sI --- LIVER I LORI 2.0751 15.8591 .4942 x 1.0000 WEI LUNG ILU) 1.4231 3.7770 % .3389 % 7.6429 WEI LF- TESTIS AT@ TESTIS TEO) TEIISO) 1.0496 .440S % GIse WEI .8913 WEI ACCESS/SEX 010 1.8101 2.8944 .4311 % 6003 % .0723 WEI -----------------------------------------------------------------------------------------1-.3-9-4-1------------W-EI------- ORGAN NAME -------------GENERAL COMMENT KEYWORDS, SEVERITY, P ADISTTRHIBU0TILON0 0 Y 0 8 S E P V A T 1 0 N S 1%9.* GROSS FitEE-TEXT COMMENTS / HISTOPATHOLOGIC -------------------------------------------- 04'OCSI:-ALi iliS6Ei/6lt6AiS'NbRitAi . . . . . . . . . . . . . . . . . ... . . THE FOLLOWING TISSUES WERE-UNREMARKABLE "UlItAEI I AT MICROSCOPIC EXAMINATION: - - - - - - - - - - - - - - NHRI"Itu'iCIIULATING OLD ICGI. LF. TESTIS (TEO). LIVER (LI), LUNG (LU). PROSTATE 1PItI.SEMINAL VESICLE --------------------------------w ------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES . AMERICA, INC. MADISON. WISCONSIN U S.A. ---------------------------------------------------------------------------------------------------------------- AN104AL NUMBER: C67477 DATE OF DEATH:OF01425/91 SEX: MALE DOSE 13ROUP: I SACRIFICE STATUS: SCHEDULED FIRST INTERIM DATE AND TIME STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 ECROPSY: 01(2S/91 9:31 PROSECTOR: NANCY DIEDRICH TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-EI-G-H-ER-:---P-A-TH-O-L-OG-Y---D --------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT IGRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ---------------------BRAIN 0 -------- TO BOD-Y--W-E-IG-H-T- 1%) WEIGHT RATIO -------- ST --- LIVER ILRIJ l.e0s?9 1 Is. 78 .4601 1.0000 WET LUNG ILU) 1.6197 4.0414 '%X .3$Sf X 1.0716 WEI LF. TESTIIS ITE RT. TEST S ITE0II 1.4154 .3360 % .8273 WEI .7229 WEI -------A-C-C-ES-S-/-SE-X----0-0-0----Sol --------------2.1.-..33.99.3608.....................53.6.3.92.6.5_..%x. .7134 WEI ..........-.-.-1-.-2-22-2-------------WE-I---- ORGAN NAME P A T " 0 L 0 0 Y 0 8 S E R V A T 10 N S A a * KEYWORDS. SEVERITY. DISTRIBUTION ----------------------------------------------------------G-RO-S-S FREE-TEXT COMMENTS / "ISTOPATHOLOGIC GENERAL COMMENT --------------------------------------------- 04(OICSI-:ALi iliS6Ei/6R6A*NS*N6Ri4Ai THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BURRAEITNHRA(BR(IUAICOAGULATING GLD ICGI. LF. TESTIS (TEO), LIVER (LI). LUNG ILU). PROSTATE IPRI, SEMINAL VESICLE ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67478 DATE OF DEATH: 02/14/91 SEX: MALE DOSE GROUP: I STUDY DAY OF DEATH: 57 SACRIFICE STATUS: SCHEDULED. SECOND INTERIM DATE AND TIME OF NECROPSY: 02/14/01 8:01 STUDY WEEK OF DEATH: 9 PROSECTOR: DENDNeIS NOFF14AN TERMINAL BODY WEIGHT: 4 POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: . TOM PALMER RECORDER: JILL BEVAN WEIGHER: VAL QUINN ----------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO SPAIN 0R IGNAMS) TO BODY WEIGHT 1%) ---------------------- -------- -------- WEIGHT RA710 -------- ST ---- BRAIN 0 LIVER ILRIJ 2.0505 16.0694 .4325 % 1.0000 WEIG LUNG (LU) 1.6043 3.5140 % .3384 % 8.1294 WEIG .7124 LATF@ TTEESSTTIISS ITTEE O11 1.6764 1.6448 .3530 % WEIG .8170 WEIG ACCESS/SEX 0 0 SO) 3.4645 ------------------------------7---- .3409 % .7308 x .1021 WEIO 1.8890 WEIG ------------------------------------------------------------------------------ ORGAN NAME a b 6 P A T H 0 L 0-0 Y KEYWORDS, SEVERITY. DISTRIBUTION ----------------- 0 8 S E R V A T 10 N S A * A GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC GENERAL COMMENT -------------------------------------------------------------------------n ---------- I(olcs-lA:Li iliS6Ei/bit6AiS'Ni)RkAi - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - 1UR3ERTAIHNRAIBNIIU,,COAGULATING OLD (COI. LF. TESTIS (TEO). LIVER (LI). LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA MAZLE70N LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CB?479 DATE OF DEATH: 05417191 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE AND TIME OF ECROPSY: STUDY DAY OF DEATH: 140 OS 17/91 1:41 PROSECTOR: STUDY RUTH WEEK OF DEATH: SCHNITZ 22 TERMINAL 600Y WEIGHT: 480.7 POST-FIX WEIGHER: NOT AVAILAO(E PAT14OLO(IlST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE --------------------------------------------------------------------------------W-E-IG-H-E-R:---D-E-NN-I-S--H-O-FF-M-A-N ------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 AG A --------------------BRAIN 1L871 -------2.0754 -------- WEIGHT RATIO -------- S TA T -------- LIVER LUNG fLU) Lf. TESTIS 14.3551 1.4153 1.5307 .4238 % 2.9316 % .3033 x 1.0000 6.9171 .7157 WEIGHT T WEIGHT T WEIGHT 7 NT. TESTIS ITTEE?L ACCESS/SEX 0 G l.sloi .3126 % .3227 % .73?S .7613 WEIOHT T WEIGHT T ------- ---------S-O-)--------------2-.4-7-9-9-------------------.5-0-6-4--% --------------1.-1-9-4-9------------W-EI-G-H-T --T-------------- ORGAN NAME PA TN0 L 00 V 0 8S E RV AT 10 NS AA A KEYWORDS. ;E*VEROITY. DISTRIBUTION ----------------------------------------------------------G-RO-S-S FREE-TEXT COMMENTS / "ISTOPAT"OLOGIC FIND GENERAL COMMENT ------------------------------------------------- GlaoCSI: -AL@ il@S6Ei/6lt6AiS'N6Rki LIVER (LI) . . . . . . . . . . . . . . . . . . . . . . . . . . .. .. . "ICRO: -MINIMAL. FOCAL PROSTATE (PR) . . . . . . . . . . . . . . . . . . . . . . . . -.li.Fi.Li.lt.AT.iS'M6N6N6CiEAR*CiLi MICRO: -SLIGHT, MULTI-FOCAL -INFLAWAT10i. *CAR6NiC'A6TiVi . . . . . . . THE FOLLOWING - - -- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - --------BRA-I-N--I-B-R-I,--C-O-A-GU-L-A-T-IN-G---OL-D--I-C-O-).--L-F-.--T-ES-T-I-S--(-T-EO-)-.--L-U-NG---IL-U-I-.--SE-M-I-N-A-L --VE-S-I-C-LE---(-SV-I-.--UR-E-T-H-R-A --(-U-R-)------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. pm -------------------------------------------------------------------------------------------------------------------- ADANIMAL NUMBER: C67480 SEX: 04ALE DOSE GROUP: I TE OF DEAT":00F24EI2/91 STUDY DAY OF DEATH: 55 DATE AND TIME CROPSY: 02(12/91 7:Si SACRIFICE STATUS: SCHEDULED. SECONDI INTERIM SA STUDY WEEK OF DEATH: I TERMINAL BODY WE G"T: 4so. POST-FIX WE113HER: NOT AVAILAS E PITOSECTORI KEVIN BILLINGS PATHOLOO :ST: DR. TOM PALMER RECORDER: DENNIS HOFFMAN --------------------------------------------------------------------------------W-EI-G-H-ER-:---P-AT-H-O-LO-G-Y---DE-P-A-R ---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R 0 ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T BRAIN (OR) LIVER ILI) 2.1222 .4624 % 1.0000 ------WEIO"T 16.4500 1.3140 3.5139 % 7.7SI4 WEIGHT LIFUNGTEILTUII '(TEOI RT. TESTIS OATEII 1.7231 .30is 1 .3754 % .6522 .8119 WEIGHT WEIGHT ACCESS/SEX G (SO) 1.1252 2.$279 .3?59 % .6161 % .8129 1.3325 WEIGHT -----------------------------------------------------------------------------------------------W-E-IG-H-T ----------------- ORGAN NAME KEYWORDS. ;EV'ER6ITY. P ADISTTR"IBU0TILON0 0 Y 0 0 S E R V A T 1 0 N S a a a ----------------------------------------------------I------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---"I-S-T-O-PA-T-H-OL-O-G-IC----F-IN-E------ GENERAL COMMENT JOCI . . . . . . . . . . . . . . . . . ..... . . . . . . . . . . . . . . . . . . . . . . . . . LIVER (LI) G OS : -ALL TISSUES/ORGANS NORMAL THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN IBR). COAGULATING GLD ICO). LF. TESTIS (TEO). LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE (SVI, URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67411 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. SECOND INTERIM SACI DATE OF DEATH: STUDY DAY OF DEATH: 55 STUDY WEEK OF DEATH: I DATE AND TIME OF EC OPSY: 02(12/91 9:07 PROSECTOR: KEVIN BILLINGS TERMINAL BODY WEIGHT: 435.1 POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: ON. TOM PALMER RECORDER: KEVIN BILLINGS --------------------------------------------------------------------------------W-EI-G-HE-R-:---P-AT-H-OL-O-G-Y --D-E-P-AR-T----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN WEIGHT RATIO ---------------------- -------- -------- -------- BRAIN (OR) LIVER fLI) 2.0951 15.4762 .4$IS % 3.5569 % 1.0000 7.3869 LUNG ILU) 1.5414 .3543 % .7357 LF. TESTIS (TEOI 1.5811 .3652 X .7583 #tT. TESTIS ITEII ACCESS/! SO) 1.6948 2.5276 .3095 % lot$ ------------------------------------------- ------- - - - - - - --s-o-o-o--%-- - - - - --- 1.'2064 0 R0 A S TA Tt --------- WEIGHT TA WEIGHT TA WEIGHT TA WEIGHT TA WEIGHT TA WEIGHT TA ORGAN NAME P A T N 0 L0 0 Y KEYWORDS. ;EOVERITY, DISTRIBUTION 0 8 S E R V A T 1 0 N S ---;-;-;---------------------- ----------------------------------------------------------G--R-O--S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-HO-L-O-GI-C----FI-N-D-I------- GENERAL C"ENT LIVER (LI) GI(OICSI: -ALL TISS6[i/61tdAiS'N61ti4A'L M.ICRO:. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -MINIMAL. FOCAL -INFILTRATES.MOI#ONUCLEAR CELL THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN IBRI, COAGULATING OLD (COI, LF. TESTIS (TEO). LUNG ILU), PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA (U ---------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. pa -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?412 SEX: MALE DOSE GROUP: I DATE OF DEATH: 03122101 STUDY DAY OF DEATH: 93 STUDY WEEK OFSACDREIAFTIHC:E 14STATUS: SCHEDULED. TERMINAL SACRIFIC DATE AND TIME OF NECROPSY: 03(22/91 9:19 POST-FIX WEIGHER: NOT AVAILAO E KEVIN BILLINGS TERMINAL BODY WEIGHT: sit. RECORDER: JOE N. KIZZIEE PRAOTSHEOCLTOOGRI:ST: DR. T014 PALMER -------------------------------------------------------------------------W-E-I-GH-E-R-:--J-I-L-L---EV-A-N------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ---------------------- BRAIN JOR) LIVER ILTI -------- 2.1415 10.2911 TO BODY WEIGHT 1%) -------.4198 % WEIGHT RATIO -------110000 STA T -------WEIGHT T LUNG ILU) LF. TESTIS OITEOISOI 1.6587 3.1148 % .3241 % 7.5161 .7720 WEIGHT WEIGHT TI AT. TESTIS TEI ACCESS/SEX G 1.9007 1.1997 3.1952 .3714 % .3712 % .0243 X .8847 .1142 WEIGHT I WEIGHT T 1.4872 WEIGHT T ORGAN---N-AM-E--------------KEYWO?tos. SEVERITY. P DAISTTRHIBU0TILON0 G Y' 0 0S ER VA T 10N S A* a GROSS FREE-TEXT COMMENTS / --------------------------------------- HISTOPATHOLOG[6 FIND GENERAL COMMENT c ------------------------------------------------- GIGOSI: -iL@-iliS6Ei/6RdA'NS'N*OR'NAL' ..... . . . . . . . . . . . . . . . . . . . . . . . . . THE- FO-LLO-WING- T-ISS-UES WERE UNREMARKABLE - AT- MI-CRO-SCOPIC .EiAMiNATION -- - - - - - - - - - - - - - - 13RAIN OR) COAGULATING GLD (CGI. LF. TESTIS ITEO) -------U-RETHRI (UAI LIVER ILIJ. LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE ISV ---------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67483 SEX: MALE DOSE (IROUP: I DATE OF DEATH: 05/16/91 DATE AND TIME OF NECROPSY; STUDY DAY OF DEATH: 148 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: POST-FIX WEIGHER: NOT AVAILOASB(lCe/ol 8:00 PROSECTOR: JULIE A. LARSEN PATHOLOGIST: DR. TOM PALMER zzGIs. RECORDER: JOE M. KI IEE --------------------------------------------------------------------------------W-E-I-GH-E-R-: --J-A-C-K --S-ER-F-OR-T------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE OROAN TO BRAIN ---------------------- -------- TO BOD-Y--W-E-IG-H-T- 1%) WEIGHT RATIO BRAIN I LIVER (OLRil 2.2214 .3610 % -------1.0000 LUNG ILUI 19.7955 3.2167 X 1.9113 LF. TESTIS TEO,ISO. 1.5677 1.7633 .2S4? Z .?OS7 RT. TESTIS OITE ACCESS/SEX 0 1.7407 .2105 % .2829 % .7931 .7138 -------------------------------------3-.7-5-8-5-------------------.6-1-0-7--% --------------1-.0-9-2-0-------- 0 Ra s TA T ------- WEIGHT WEIGHT WEIGHT WEIGHT WEIGHT WEIGHT ORGAN NAME P A T " 0 L 0 (1Y' 0 8 S E R V A T 1 0 N S ----------------------KEYWORDS. ;EVAERaIIY. DISTRIBUTION ;-;-;--------------------- ------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND GENERAL COMMENT c ------------------------------------------------- Glaost: -ALL iliS6Ei/6R6AiS*N6lt*MA@ LIVER (LI) . M.IC.RO.: .-M.IN.IM.AL.. .FO.CA.L . . . . . . . . . . . . . . . . . . -INFLAMMATiOi.dRAN6L6MAT6Ui THE FOLLOWINO . . . . . .. - - - - T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - 13RAIN ISR). COAGULATING OLD (CO), LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE -----------------------------------------------------------------------------------------(-S-V-I-.-U-R-ET-H-R-A --I---------------- INDIVIDUAL APPENDIX D ANlt4AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C@7484 DATE Of DEATH: 02/12/91 SEX: 14ALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA STUDY DAY OF DEATH: 55 DATE AND TIME OF NECROPSY: 0A 2(12/91 1:13 STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: 416. POST-FIX WEIGHER: NOT AVAIL 0 E PROSECTOlt: NANCY DIEDRICH ECONDER: DENNIS HOFFMAN --------------------------------------PA-T-H-OL-O-GI-S-T-:---DR-.--T-O-M--P-AL-M-E-R ----------------WR-EI-G-HE-R-:---P-AT-H-OL-O-G-Y --D-E-P-AR---------------- OROAN NAME ABSOLUTE ORGAN WEIGHT JORANS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Aa --------------------BRAIN OR -------2.1016 TO BODY WEIGHT 1%) -------.4332 % WEIGHT RATIO -------1.0000 S TA T -------WEIGHT T LIVER ILZI LUNG LU 11.2SI3 1.7544 3.7512 % .2005 X I.SSIS .1320 WEIGHT TI LF. TEITII EO11 OT. TESTIS JTTE 1.7484 .3592 % .1292 WEIGHT WEIGHT T ACCESS/SEX ------- 0 SO) 1.7861 2.9183 .3670 % .6140 % .8471 1.4172 WEIGHT T --- -----------------------------------------------------------------------------W-EI-G-HT---T-------------- OROAN NAME P A T H 0 L 0 0 Y 0 0 S E A V A T 10 H S 6 0 6 ---------------------K--E-Y-WO-R-DS-,---;-E-AV-E-RO-I-T-Y,--DI-S-T-RI-B-U-TI-O-N----------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND GENERAL COMMENT c ------------------------------------------------- 01(olst:-ALi iliS6Ei/bltdAiS'N6RkA@ THE FOLLOWING TISSUES WERE UNREMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BUNREATIHNR ION()U.RICOAOULATING OLD (CO). LF. TESTIS (TEOP, LIVER ILIJ. LUNG ILU). PROSTATE IPRI. SEMINAL VESICLE ISV --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. pa --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C674$S DATE OF DEATH: 03/22/91 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED. TERMINAL SACRIFICE STUDY DAY OF DEATH: 93 DATE AND TIME OF RECROPSY: 03(22/01 7:50 STUDY WEEK Of DEATH: 14 PROSECTON:, KEVIN BILLINGS TERMINAL BODY WEIGHT: s9s.6 P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-OT--A-V-AI-L-A-S--C----------------PA-T-HO-L-OO---S-T-:--D-R.---TO-M--P-A-L1-4-E-R ---------------WR-EEI-CGO-HR-EDR-E:R-:--J-IJL-OLE--MB-.E-V-AKN-IZ-Z-I-EE------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT JORANSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 A0 A ---------------------BRAIN JOR) -------2.2154 -------.3720 % WEIGHT RATIO -------1.0000 S TA T -------WEIGHT T LIVER ILI) LUNG ILUI LF. TESTIS RT. TESTIS ITTeElol 21.0922 1.9151 1.9466 1.0120 3.5413 % .3333 % .3208 % O.S207 .8900 .8787 WEIGHT 7 WEIGHT I WEIGHT T ACCESS/SEX 0 0 Sol 2.9746 .3210 % .4994 x .1630 1.3427 WEIGHT T -----------------------------------------------------------------------------------------------WE-I-GH-T---T ---------------- ORGAN NAME P A T " 0 L 0 0 Y. 0 0 S IEP V A T 1 0 N S a * A KEYWORDS. ;E*VERITY.DISTRIBUTION ----------------------------------------------------------G-RO-S-S FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FIND GENERAL CON14ENT ------------------------------------------------- OACOICSI-:AL@ itiS6Ei/6RdAiS*Ni)RkAi - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - - - BURREATIHNR JOR(IU,P)COAGULATING GLD (CO). LF. TESTIS (TEO). LIVER ILI). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE ISV --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NU04BER: COYAOS SEX: 14ALE DOSE GROUP: I DATE OF DEATH: 01423/91 DATE AND TIME OF STUDY DAY OF DEATH: 35 STUDY WEEK OFSACDREIAFTIHC:E S STATUS: SCHEDULED FIRST INTERIM SAC ECROPSY: 01 23/91 S:AS POS7-FIX WEIGHER: NOT AVAILAO(E PROSECTOR: NANCY DIEDRICH TERMINAL 601)Y WEIGHT: 397. ECORDER: JILL BEVAN ----------------------------------------P-AT-H-O-LO-G-I-ST-:---D-R-.--T-01-4-P-A-L-ME-R----------------WI-E-IG-H-E-R:---G-A-IL--M. NOVAK ORGAN NAME ABSOLUTE OROAN WEIGHT ORGAN WEIO"T RELATIVE ------------ ORGAN TO BRAIN ---------------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO 0Ra STA T BRAIN 2.1138 -------- ------- LIVER I8L;1 LUNG (LUI 16.3091 .5324 % 4.1081 % 1.0000 7.7iss WEIGHT LF. TESTI TEO1 l.s4ls I.S448 .3883 % .7293 WEIGHT WEIGHT RT. TESTIS ITE ACCESS/SEX ORO ISO) I.S42 .3191 % .3902 % .7308 .7329 WEIOHT ----------------------------------------------2-.-5-$-S-4----------- .6512 % 1.2231 WEIGHT T WEIGHT T OROAN NAME -------------- - GENERAL COMMENT c KEYWORDS. P A T " 0 L 0 0 V -0--8- S-E--R--V-A--T--1--0-N--S----a-a--a------------------------- EVERITY. DISTRIBUTION GROSS FREE-TEXT COMENTS / HISTOPAT"OLOGIC FIND ---- ------------------------------------------------- Gloost: -ALi itiS6Ei/61tdAhS'N61ti4AC .. . .. ... .. .. . .. .. ... .. THE FOLLOWING TISSUES WERE -UIA-RE14-ARKA-BLE- AT- MI-CROS-COP-IC - EX-AMIN-ATION: - - - - - - - - - - - - - - - BURREATHIRNA O(RU)il COAGULATIONLGDICOI,LF.TESTISITEO)L.IVER(LIP.LUNGILUI.PROSTAT(EFRI.SEMINAVLESICL(ESV --------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATOOTES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANlt4AL NUMBER: CS?487 DATE Of DEATH: 03/25/91 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHED LED TERMINAL SACRIFI STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 DATE AND TIME OF NECROPSY: 03 2SI91 8:19 PROSECTON: JULIE A. LARSEN TERMINUAL 6ODY WEIGHT: 523 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIE --------------------------------------------------------------------------------------W-E-I-(I-H-E-R-:-J-O-E--M.--K-I-Z-ZI-E-E--------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ---------------------- -------- TO BOD-Y--W-EI-G-H-T- 1%) WEIGHT RATIO -------- S IA ------ BRAIN 10L9IJ LIVER 2.3102 17.4732 AS48 % 1.0000 WEIGHT LUNG (LU) 1.573S 3'3314 % .3006 % 7,3411 WEIGHT Lf, TESTIS OITEOJISOI RT. TESTIS TE 1.7150 1.6766 .3277 % .8611 .720S WEIGHT WEIGHT ACCESS/SEX G 4.2208 .3203 % .1064 % -------------------------------------------------------------- .7044 1.7733 WEIGHT WEIGHT ----------------------------------------------------- ORGAN NAME -------------GENERAL COMMENT IGASOSCI: THE FOLLOWING TISSUES P A I H 0 L 0 (1 Y KEYWORDS. ;EAVER*ITY, DISTRIBUTION 0 6 S E R V A I 1 0 N S 0. * a GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIN ------------------------------------------------ -ALL ifisuEi/i)R6AiS'N6RitA@ . .. .. .. .. .. . .. . .. .. .. .. - -- ----- --- -- --- ---- --- --- -- WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: UBRREATIHNRI Bl(tUlR.I COAGULATING GLD (CCI). LF. TESTIS (TEO). LIVER (LIJ. LUNG (LUI. PROSTATE IPRI. SEMINAL VESICLE (S -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?488 SEX: KALE DOSE GROUP: I DATE OF DEATH: 03426/91 STUDY DAY OF DEATH: 97 DATE AND TIME OF ECROPSY: 03(26/91 7:22 STUDY WEEK OFSACDREIAFTIHC:E 14STATUS: SCHEDULED. TERMINAL SACRIFI TE OMINAL BODY WEIGHT: 621 POST -FIX WEIGHER: NOT AVAILAS E PROSECTON: STEVE VAN ADESTINE ECORDER: JILL BEVAN ---------------------------------------PA-T-HO-L-O-GI-S-T-: ---DO-.--T-O-M--P-AL-M-ER-----------------IWE-I-G-H-ER-:---JO-E--N. KIZZIEE ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE -------------------ORGAN TO 13RAIN ------------------- JORAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO 0 Ra S TA BRAIN JOR) LIVER (LI) 2.2904 22.4033 .3017 % -------1.0000 -----WEIGHT LUNG ILU) 1.7742 3.6101 % 9.1076 WEIGHT Lf. TESTTII,SS OITEO ISO. 1.6540 .2856 % .7748 Wlio"t AT. TE TE ACCESS/S X a 1.623S .2663 % .2613 % .?221 .7018 WEIGHT ------- -----------------------------3-.-99-0-1-------------------.-6-42-3--%--------------1-.-74-2-1------------WW-EE-IIGG-HH-TT--------------- ORGAN NAME P A T H 0 L o a Y' 0 0 S E N V A I 1 0 N S * a * ---------------------K--E-Y-WO-R-D-S,---*S-E-Vm-ER-*I-TY-.---D-IS-T-R-IB-U-T-IO-N----------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl GENERAL COMMENT ------------------------------------------------ (il'o'st:-ALi iliS6Et/6R6AiS'N6Ri4Ai . .. .. .. . .. .. .. THE- FO-LLOW-ING- -TISSUES WERE -UNREMARKABLE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - UBRRETAHIRAN(O(NUi)lCOAGULATIONLGDICOI.LF.TESTIS(TEO).LIVERILI).LUNO(LU).PROSTAT(EPRI.SEMINAVLESICL(ES ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COYASO DATE Of DEATH: OF01424@09PI SEX: MALE DOSE GROUP: I STUDY DAY OF DEATH: 36 SACRIFICE STATUS: SCHEDULED FIRST INTER114 SAC DATE AND TIME EC SY: /24/91 9:27 STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: 382. POST-FIX WEIGHER: NOT AVAILAoBlLE PROSECTOI:,KEVIN BILLINGS PATHOLOO S : DR. TOM PALMER RECORDER: VAL QUINN WEIGHE -------------------------------------------------------------------------------------R-:--D-E-BB-I-E--P-I-R-KE-L------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra -------------------BRAIN JORI -------- TO BOD-Y--W-EI-G-H-T- 1%) WEI-O-"T---R-AT-I-O S TA T ------- LIVER ILIP 2.1238 14.7940 .5550 % 3.l6s9 % 1.0000 6.9602 WEIGHT LUNG ILU) LF. TESTIS ITTEEO, .1788 1.026S .2290 % .4138 WEIGHT WEIGHT RT. TESTIS 1.7403 .4773 % .4561 % .1400 WEIGHT ------A-C-CE-S-S-/S-E-X---0--0--I-S-O-)--------------2.-6-7-6-9-------------------.6-9-2-5 -%---------------1.-.28-02-034-2------------WW-EEII-GG-HHTT------------------ ORGAN NAME PA TN0 L0 0 r 0 8S ER VA T 10 NS a* * KEYWORDS. ;EAVERITY, DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENTS / "ISTOPAT"OLO(IIC FINE GENERAL COMMENT ------------------------------------------------ LIVER ILI) GI(OICSI-:ALi itiS6Ei/6R6AiS'N6RAA@ M16R6: '-Alili4A@. :IiFiLiRATiS'M6N6N6CiEAR*CiL@ - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - -------B-R-A-IN---IO-R-)-.-C-O-A-G-U-LA-T-I-N-G --O-LD--I-C-O-)-. --LF-.--T-E-ST-I-S--I-T-E-OI-.--L-U-N-G--(-LU-)-.--P-R-OS-T-A-T-E --(-PR-)-.--S-EM-I-N-A-L--V-ES-I-C-L-E--I-S-VI-.--U-R-ET-H-RA---I---------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL HUMBER: C$7490 DATE OF DEATH: OS 16vi SEX: MALE DOSE GROUP: I STUDY DAY OF DEATH: 14$ SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE AND TIME OF 4EC OPSY: 05 16/91 7:11 STUDY WEEK OF DEATH: 22 PROSECTOlt: (IAIL M. NOVAK TERMINAL 6ODY WEIGHT: $10.6 POS-T---FI-X--W-E-I-GH-E-R-:--N-O-T--AV-A-I-LA-B-(-E----------------P-A-TH-O-L-OG-I-S-T:---O-N-.--T-OM--P-A-L-ME-R----------------l-wE-tC-EOIN-GD-HEE-RR:-:-J-AJ-COK-E --NS ,-I-TF-K'-ON-Z'T-I-EC----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R0 A ---------------------BRAIN ION) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T -------- LIVER (LI) 2.0230 19.8409 .3313 % 1.0000 WEIGHT T LUNG ILU) LF. TESTIS TE IT. TESTIS TEI0 1.6371 1.7120 1.1683 3.2494 % .2681 % .2918 % .3060 % 9.1077 .8092 .1809 .9235 WEIGHT T WEIGHT T WEIGHT I ------A-C-CE-S-S-/S-E-X---0-10---I-S-OI---------------3.-8-6-43--------------------.0-3-32 % 1.9112 WEIOHT T WEIGHT T ------------------------------------------------------- ORGAN NAME KEYWORDS, ;E*VER*ITYP. DAISTTRHIBU0TILON0 0 Y 0 0 S E A V A T 1 0 N S * & 0 -------------------------------------------------------- GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC FIND GENERAL COMMENT 7 --- ------------------------------------------------- LIVER (LI) THE-FO-LLOW-ING (il'O'SI-:ALi iliS6Ei/6R4AiS'N6Ri4AE . . . . .. .. .. ... . .. .. . ... ... .. .. .. . ... . ... .. . .. .. .. MICRO: -SLIGHT, FOCAL -INFILTRATiS:M6N6N6CiEAR*CiLi . . . . . . . . . . TISS-UES -WE-RE-UNNE-MARK-AOL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATION: -- --- --- --- --- BRAIN (OR). COAGULATING OLD ICOI. LF. TESTIS ITEO). LUNG ILU). PROSTATE IPRI,.SEMINAL VESICLE ISVI. URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HMAAZDLIESTOONN, WLIASBCOORNATSOIRNIES U.SA.MAER.ICA. INC. Pit -------------------------------------------------------------------------------------------------------------------- AP#tl4AL NUMBER: C87491 SEX: 04ALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE OF DEATV:OfOI423/91 STUDY DAY OF DEATH: 35 DATE AND TIM ECROPSY: 01 23/91 1:57 STUDY WEEK OF DEATH: 5 TERMINAL 6ODY WEIGHT: 381. POST-FIX WEIGHER: NOT AVAILAO(E PROSECTON: NANCY DIEDRIC14 PATHOLOGIST: DR, TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-EI-G-H-ER-:---G-AI-L--M-.--N-OV-A-K------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ---------------------- -------- TO BOD-Y--W-EI-G-H-T- WEIGHT RATIO -------- S TA T ------- 011AIN (ON) 1.9387 .4995 % 1.0000 WEIGHT LIVER ILI) LUNG ILUI 14.42SO 1.4507 3.7168 % 7.4406 WEIGHT LF. TESTIS I TE 1.5008 .3738 % .3867 % .7483 .7740 WEIGHT WEIGHT RT. TESTIS TEI0 ACCESS/SEX 010 ISOI 1.5182 2.7210 .3012 % .7831 WEIGHT ------------------------------------------------------------.7-0-2-9--% --------------1-.4-0-7-1------------W-E-IG-H-T ----------------- ORGAN NAME * a* PA TH0 L00 0 0 S E ItV A T 1 0 N S . . . ---------------------K--E-Y-WO-R-D-S.---S-E-V-ER-I-TY-.---D-IS-T-R-IB-U-T-IO-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---"I-S-T-O-PA-T-H-OL-O-O-IC----FI-N------- GENERAL COMMENT GIGOCSI:-ALi itiS6Ei/6R6AiS*NbR*MAL . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . . - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - - - BURRAEITNHRA gaJlUAICOAGULATING OLD (CGI, LF. TESTIS ITEO). LIVER (Lll. LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE (S -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. ll#C. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7402 DATE DF DEATH: 01/24/91 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 38 STUDY WEEK OF DEATH: 6 (24/21 1:36 PROSECTOR I JULIE A. LARSEN TERMINAL &ODY WEIGHT: 4SI.1 POST-FIX WEIGHER: NOT AVAILAoSl E PATHOLOCI S:T: OR. TOM PALMER ECONDER: VAL QUINN -------------------------------------------------------------------------W-R-EI-G-HE-R-:--D-E-81-3-TE--P-I-RK-E-L------------------ OROAN NAME ABSOLUTE ORGAN WEIGHT (ONAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT OROAN TO BRAIN 0 R0 0 - ------------ -------- -------- WEIGHT RATIO -------- STA T -------- BRAIN JOR) LIVER (LI) 2.1730 23.3031 .4109 % 1.0000 WEIOHT T LUNG JLUI LF. TE Ti TE 1.70S4 S.IS67 % .3774 % 10.7239 .7848 WEIGHT T WEIGHT T RT. TESTIS TEI0 1.7399 1.8369 .3$SO % .40$S X .1007 WEIGHT T ------A-C-C-E-SS-/-S-E-X--0-1-0---IS-O-)--------------3-.-2461 .7183 % .1453 1.4930 WEIGHT T WEIOHT T OROAN NAME KEYWORDS. ;-;---P--A--T--H--O--L--0--0--Y-'----O--B--S--E--R--V-A--T--1--0--H--S;EVERITY. DISTRIBUTION --------------------- - ------------ - - ------- GROSS-FREE-TEXT-COW*NTS--/--HISTOPATHOLOGIC-FIND KIDNEY(SP (KD) . . . . . . . . . . . . . . . . . . . . ..... . . . . . . . . . . . . . . . ... . . . . . . . . ONOSS: -LARGE PELVISIES) MICRO: -PRESENT -RIGHT: BOTH COLLECTED LIVER (LI) -OILATATIOM,PELVIC . M.IC.RO:. .-M.OD.ERA.TE.. . F.OC.AL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -INFLAMMTION.GRANULONATOUS THE- FO-LLOW-INO- T-ISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - BRAIN IOR). COAGULATING OLD ICO), LF. TESTIS ITEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SVI, URETHRA I -------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC, MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7493 DATE OF DEATH:OOSF417@91 SEX: 14ALE DOSE GROUP: I STUDY DAY OF DEATH: 149 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE AND TIME EC OPSY: 17/91 7;03 STUDY WEEK OF DEATH: 22 PROSECTON: RUT" SCHMITZ TERMINAL body WEIGHT: sio. POST-FIX WEIGHER: NOT AVAILA0B5(E PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE WEIGHER: JACK SERFORT -------------------------------------------------------------------------------------------------------------------- ABSOLUTE OR13AN WEICIHT ORGAN NAME ---------------------- (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIOHT (U) OROAN TO BRAIN WEIGHT RATIO 0R a S TA I BRAIN B -------2.0441 5.s493 -------400S It 3:0465 % -------1.0000 ------WEIGHT LIVER Li LUNG LF. TE I FTEOI l 1.5130 1.7096 .2964 % 7.6069 .7402 WEIGHT WEIGHT AT. TE TIS ITEII ACCESS/SEX 0 0 Sol 1.1110 .33SO % .3SSO % .1344 .1164 WEIOHT WEIGHT -------------------------------------2-.9-2-S-O-------------------.-57-3-2--% --------------1-.4-3-1-2------------W-E-IO-"-T----------------- ORGAN NAME PA T "0 L0 0 V KEYWORDS. SEVERITY. DISTRIBUTION ------------------ 0 8 S ER VA T 10N S GROSS FREE-TEXT COMMENTS HISTOPATHOLOCIIC FIM SKIN (SKI --------------------------------------------------------------------------------------. . . . . . . . . . . . . . . . . . . . . . . ..... . . . . . GROSS: -ABRASIONIS)/ULCERATION(S) -MID VENTRAL'CiR@16Ai iE6l6N: iliGiE'AiRADiD'AiEA MICRO: -SEVERE, FOCAL TAN AND HOIST (CAP 61 -DEftt4ATITIS -SEVERE, FOCAL -ULCERATION THE- FBOR-LALION-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - URETHNI"(U,41 COAGULATING OLD ICO). tF. TESTIS (TEO). LIVER ILI). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE IS -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. pw -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C@7494 DATE OF DEATH: OF024EI4191 SEX: MALE OSE GROUP: I STUDY DAY Of DEATH: D SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE AND TIME CROPSY: 02(14/91 8:54 S7 STUDY WEEK OF DEATH: 9 PROSECTON: DENNIS HOFFMAN TERMINAL 6ODY WEIGHT: 4S2. POST-FIX WEIGHER: NOT AVAILAB E PAT"OLOCIIST: DR. TOM PAL14EN RECORDER: JILL BEVAN --------------------------------------------------------------------------W-E-IO-H-E-R:---V-AL--Q-U-I-NN--------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) 0110AN WEIGHT RELATIVE ORGAN TO BRAIN 0R 0 ------------------BRAIN (ON) -------- TO BOD-Y--W-EI-G-H-T- (%I WEIGHT RATIO -------- S TA T ------- LIVER (LI) 2.1980 16.IS26 4862 % 3'705S % I.OODO 7.6217 WEIGHT LUNG .(LU) Lf. TESTIS T NT. TESTIS 'ITEEOII 1.0485 1.6606 1 6441 .3646 % .3673 % .3637 X .?SOO Isss .7480 WEIGHT WEIGHT WEIGHT ------A-C-CE-S-S-/S-E-X---0-a---(-S-o-l--------------3-o-i-g-s------------1-------.-6672 % 1.3724 WEIGHT WEIGHT ------------------------------------------------------ DROAN NAME P A T " D L0 0 Y 0 9 S E P V A T 1 0 N S a a A KEYWORDS. ;E*VE'RITY. DISTRIBUTION -------------------------------------------------------G--R-O--S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-O-PA-T-H-OL-O-G-IC----FI-N------- GENERAL COMMENT PROSTATE (PR) GI(OICSI-:ALi itiS6[i/61tdAiS'146R'MA'L. . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . . M.IC.RO.: .-S.LI.GHT.. . F.OC.AL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -INFLAMMATIDN. CHRONIC ACTIVE THE FOLL&il@M -TISS-UES- WE-RE-UNNE-NAII-XABL-E - AT- MI-CROS-COP-IC - IE-XA1-41MA-TION--- - - - -------B-RAIN (OR). COAGULATINO OLD (CO). LF. TESTIS (TEO). LIVER (LI). LUNG (LU). SEMINAL VESICLE ISV). URETHRA (UR --------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC, MADISON, WISCONSIN U.S.A. PR --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7495 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED SECOND INTERIM SAC DATE OF DEATH:00F2412/91 STUDY DAY OF DEATH: 55 DATE AND TIME ECROPSY: 02(12/91 9:11 STUDY WEEK OF DEATH: I TE RHINAL 6ODY WEIOHT: 4$S.5 POST-FIX WEIGHER: NOT AVAILAS E PROSECTOR: RUTH SCHMITZ PATHOLOGIST: DR. TOM PALMER RECORDER: DENNIS HOFFMAN -------------------------------------------------------------------------------W-E-IO-H-ER-:---P-A-TH-O-LO-G-Y---DE-P-A-RT----------------- ORGAN NAME -------------------- ABSOLUTE ORGAN WEIGHT IGRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT OROAN TO BRAIN WEIGHT RATIO 0 R0A STA T -------- -------- -------- -------- BRAIN ILO1Otl LIVER 2.0117 18.0970 .4151 % 1.0000 WEIGHT T LUNG (LU) LF. YE tl$ 1.6054 3.7275 % .3430 % 8.9647 .1250 WEIGHT T WEIGHT T STIES OITEO ISO, RT. TE TE 1.6692 1.7320 .3438 % .1269 WEIOHT T ------A-C-C-ES-S-/-S --X----0-------------------3.-4-8-9-3-------------------.7-1-8-7--% --------------1-..71-25-68-50------------WW-EEII-GG-HHTT---TI---------------- ORGAN NAME PA IH0 L0 0 r KEYWORDS. ;EV*E0RITY. DISTRIBUTION ---------------------- 0 0 SE RV A T10 N S a aa GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND ---------------------------------------------------------------------------------------- OENERAL COMMENT LUNG (LU) GAOOCSI:-ALi ifiS6Ei/6R6AiS'N6*kA@ .. ... .. ... .. .. .. .. .. .. .. .. . .. .. . ... .. . GROSS: NECROPSY NOTE: )TRIMMING COMMENT: LUNO iEdTiOi OF TRIMMING MICRO: -TISSUE MISSING i4liSiN6 ift6M'WiT*T THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE AT MICROSCOPIC EXAMINATION: BRAIN [BR). COAGULATING OLD ICOP. LF. TESTIS ITEO). LIVER (LI), PROSTATE IPAI, SEMINAL VESICLE (SVI. URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NLN40ER: CG?496 SEX: MALE DOSE GROUP: I DATE OF DEATH: 05 16491 STUDY DAY OF DEATH: 141 STUDY WEEK OFSACDREIAFTIHC:E 22STATUS: SCHEDULED FIRST POST-ITIECOV DATE AND TIME OF 4EC OPSY: 05/16/91 7:2S PROSECTOR: JULIE A. LARSEN TERMINAL 6ODY WEIGHT: S07.2 POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER ITECONDER: JOE N. KIZZIEE -----------------------------------------------------------------------------WE-I-G-HE-R-:---JA-C-K--S-ER-F-O-RT---,----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIOHT RELATIVE ORGAN TO BRAIN 0N 0A -------------------- TO BODY WEIGHT -- ----- WEIGHT RATIO ---- --- STA T BRAIN (BRI LIVER ILI) 2.0397 .4021 % 1.0000 -------- LUNG (LU) LF. TESTIS '(TEO IS.7533 I.SO19 1.7336 3.IOSS % .2961 % .3411 % 7.7233 .7363 WEIGHT T WEIGHT T WEIGHT T AT. TESTIS ACCESS/six 04011, Sol 1.8395 .3027 % .8499 .9018 WEIGHT TI WEIGHT -------------------------------------3.-2-3-0-6-------------------6-3-6-9 --% ---- 1.5839 WEIGHT TO ------------------------------------------------ ORGAN NAME P A T H 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S a ft* -------------- KEYWORDS. ;E*VERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FINDI GENERAL COK*NT -------------------------------------------------- GAGOCSI:-ALi iliS6[i/6R6AiS'N60AAi . . . . .. .. ... . .. .. .. . .. ... .. . GENERAL INFORMATION (XXI . . . . . . . . . . . . . . . . ORO ss NECROPSY tooiIE' 'WAlti-ElkIE'LiOSN-i (-HEA- - I#O.TiD.lk6LiNiCAL'OiSilt4Ai INADVERTENTLY NOT C=NTED ON AS TO ABSENCE OR PA -- - - - - - -- - - - - - OF NECROPSY THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC UIPlEITt"AIlt"lll(IU EXAMINATION: -- ---- ---- -- ---- , ICOAGULATING OLD ICO). LF. TESTIS (TEO). LIVER (LI). LUNG (LU). PROSTATE (pit).SEMINAL VESICLE (SVI ---------------------------------------------------------------------------------------------------------------------- APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?497 SEX: MALE DOSE GROUP: I gAtE OF DEATH: OS/17/91 STUDY DAY OF DEATH: 149 DATE AND TIME OF NECROPSY: 05 SACRIFICE STATUS: STUDY WEEK OF DEATH: 22 1?/Ol 7:27 POST-FIX WEIGHER: NOT AVAtLAe(E PROSECTON: NUT" SCHNITZ PATHOLOGIST: DR. TOM PALMER SCHEDULED FIRST POST-RECOV TERMINAL AODY WEIGHT: S97.7 RECORDER: JOE M. KIZZIEE WEIGHER: JACK SERFORT ORGAN NAME ABSOLUTE ORGAN WEIGHT DROAN WEIGHT RELATIVE ORGAN TO BRAIN ---------------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO 0R 0A S TA T BRAIN ferl 2.2377 --- -------- LIVER ILI) LUNG (LU) LF. TESTIS ie.ooss 1.5723 1.0288 .3744 % 3.1791 % .2031 % 1.0000 8.4033 .7026 WEIGHT T WEIGHT T WEIGHT T ItT. TESTIS ITT'Eol ACCESS/SEX 0 0 ISO) 1.664S .272S % .2785 z .7279 .7438 WEIGHT T -------------------------------------3-.-93-7-2-------------------.-65-1-7--%--------------I---IS-9-5------------WW-EE-IIGG-HH-TT---TT --------------- O-R-GA-N--N-A-M-E -------------K-EYWORDS. P A T N 0 L 0 (1Y' 0 8 S E R V A T 1 0 N S ;E*VERITY, DISTRIBUTION aaa ------------------------------------G-R-OS-S FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FIND LIVER ILI) . . . . . . . . . . . . . . . . . . . . . . . ------------------------------------------------- MICRO: -SLIGHT. FOCAL SPLEEN ISPI . . ... .. dR6Si:'-6YiTiSi - - - - - - -MICITO-: -PRESENT - -- - - :VitAiNAL'S6RiA6E:iliffliE'CiSi.*2'MkiN.DiAilEiEi.'r CLEAR FLUID ICOLLECTED) -CYST. SEROSAL THE FOLLOWING TISSUES WERE UllitEt4ARKAOEL AT N ICRC-)SCO-PIC- EX-AMI-NATI-ON:- - - - - - - - - - - - - - - --------BR-AIN ION). COAGULATING OLD (COI. LF. TESTIS (TEOI. LUNG (LU), PROSTATE (Piti. SEMINAL VESICLE ISVI. URETHRA (t --------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C@7491 SEX: MALE DOSE GROUP: I DA E OF DEATH: 02/13/91 STUDY DAY OF DEATH: 56 DATE AND TIME OF NECROPSY: 02 13/91 ?:So SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA STUDY WEEK OF DEATH; I TERMINAL AODY WEIGHT: SOO, POST-FIX WEIGHER: NOT AVAILAB(E PROSECTOR: DENNIS HOFF14AN RECORDER:,IEVIN BILLINGS ------------------------------------------P-AT-H-O-LO-G-I-ST-:---D-R-. --T-00-4-P-A-L1-4-E-R---------------W-E-IG-H-ER-:-----L-L--BE-V-A-N -------------------- OROAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra --------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- SIA I ------- BRAIN (OR) LIVER (LI) LUNO (LU) 2.ISSG 21-5017 1.5132 .4260 % 4.2SO? % 1.0000 9.9781 WETONT WEIOHT LF. TESTIS AT. TESTIS TEL 1.5765 .3129 % .3116 % .734S .7314 WEIGHT WEIGHT ACCESS/SEX olatiolsol I.S?39 3.3224 .3110 % .6s$s % .7301 1.5413 WEIGHT -----------------------------------------------------------------------------------------------W-E-I-O"-T---T --------------- ORGAN NAME P A T H 0 L 0 0 Y, 0 0 S E R V A T 1 0 N S 0 ita KEYWORDS. ;E*VEaRITY. DISTRIBUTION ----------------------------------------------------------B-R-OS-S FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FIND LUNG ILU) . . ------------------------------------------------- 6R6S@:'-t4OtTiE6 * ' * * . MICRO: -UNREMARKABLE * * ' ' * * ' 'ALiL *L6OSE1S:* ARED6 AAND6 6DAR1K 'RiED ' ' * . . . . . . . HISTOPATHOLOGICAL COMMENT: )-NO MICROSCOPIC CORRELATE TO GROSS OBSERVATION. - -TH-EF-OLL-OW-INT-GIS-SU-EWSE-RE-UN-RE-MA-RKA-BAL-TEM-ICR-OS-CO-PEI-XCAM-IN-AT-IO-N-- - - - - - - - - - - - - BRAIN(OR),COAOULATIGNLGO(CO).LF.TESTIS(TEO)L.IVER(LI).PROSTAT(EPRI.SEMINAVLESICLEISVI.URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C07490 SEX: MALE DOSE GROUP: I DATE OF DEATH: OF03426191 STUDY DAY OF DEATH: 97 DATE AND TIME ECROPSY: 03 26/91 8:27 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: Sly.0 PGST-FIX WEIGHER: NOT AVAILAO(E PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN ------------------------------------------P-AT-H-OL-O-C-II-S-T-: --D-R.---TO-M--P-A-LM-E-R----------------W-E-IG-H-E-R:---J-O-E --H.--K-I-ZZ-I-E-E------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R0 A --------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T BRAIN ILB1R1 LIVER 2.2040 18.5020 .3120 % 1.0000 -------WEIGHT T LUNG ILU, LF. TE tIS TE 1.6464 3.2066 % .28S3 % 2.3947 .7470 WEIO"T T WEIGHT TO PT. TESTIS 'TEI ISO 1 1.8157 1.3028 .3147 % .3124 % .8231 .8110 WEIGHT TO -------AC-C-ES-S-/-SE-X---0--Q-------------------3-.8-6-0-0-------------------.-66-9-0--% --------------1-.-7S-I-4------------WW-EE-IIGG-HH-TT --TT-OO---------------- OROAN NAME P A T " 0 L 0 0 Y- 0 B S E R V A I 1 0 N S Ita a KEYWORDS. ;E*VERITY, DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOOIC FINDI GENERAL COMMENT -------------------------------------------------- 0400CSI:-AL@ iliS6Ei/6R6AhS*N6Rki - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - THE FOLLOWING TISSUES WERE UNRE14ARKABLE AT MICROSCOPIC EXAMINATION: BURREATIHNRA IB(RUIR.I COAOULATING GLO (COI. LF. TESTIS (TEO). LIVER (LIP, LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE (SVI ---------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AHEITICA. INC. KADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NU14BER: C$7500 DATE OF DEATH: 01/23/91 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED STUDY DAY OF DEATH: 35 STUDY WEEK OF DEATH: 5 FIRST INTERIM SAC DATE AND TIME OF NECROPSY: PROSECTO!: DENNIS HOFF14AN TERMINAL 600Y WEIGHT: 401. POST-FIX WEIGHER; NOT AVAILA0S1(E23/21 7:50 PATHOLOO ST: DR. TON PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-IG-H-E-R-: --G-A-IL--M-.--N-O-VA-K------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 Na 0 ---------------------- -------- -------- WEIGHT RATIO -------- S TAT -------- BRAIN 18*1 LIVER ILI) 2.0014 15.5389 .4912 % 1.0000 WEIGHT 7 LUNG 5614 3.8613 % .3187 % 7.7640 WEIGHT 7 LF. TESTI RT. TESTIS TE TEIISO, I1.-6913 1.693S .4210 % .7802 .8451 WEIGHT T WEIGHT T ACCESS/SEX 0 a 2.4641 .4216 % .6136 % .1462 1.231S WEIGHT T WEIGHT T --------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 0 Y' 0 9 S E N V A T 1 0 N S a @%9. XEYWORDS. ;E*VERITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-IC----FI-N-D------- GENERAL COMMENT c (ilaost:-AL@ iliS6Ei/bRdAiS'N6Rt@A@ .. . .. ... . .. .. -- - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BURNAEITNHRI On()Ui)COAGULATING OLD (CO). LF. TESTIS (TEOP, LIVER (LI), LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE ISV --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HMAAZDLIESTOONN. WLIASBCOORNATSOIRNIES U.SA.MAER.ICA, INC. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUM13EN: C67501 SEX: 14ALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF DEATH: 03/25/91 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMI NAL 6ODY WEIGHT: 511. DATE AND TIME OF NECROPSY: 03(2S/91 9:31 PROSECTON: JULIE A. LARSEN RECORDER: JOE M. KIZZIEE P-O-S-T--F-I-X --W-EI-G-H-ER-:---NO-T--A-V-AI-L-A-O--E----------------PA-T-H-OL-O-G-IS-T-:---0-4.--T-O-M--P-AL-M-E-R----------------W-EI-G-HE-R-:---S-TE-V-E--V-A-N-A-D-E-ST----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ---------------------- -------- TO BOD-Y--W-EI-G-H-T - WEI-GH-T---R-AT-I-O S TA T ------- BRAIN 1L0I0J LIVER LUNG ILU) 2.1125 11.9192 1.6087 4271 % 3.* 1024 % .3148 % 1.0000 0.8616 .7371 WEIGHT WEIGHT WEIGHT LF. TESTIS RT. TESTIS TEIISO) l.s330 1.5214 .3000 % .2901 % .7024 WEIGHT ACCESS/SEX 0 a 3.3014 .6461 % .7003 I.SI27 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME A e a P A T " 0 L 0 0 Y* 0 0 S E R V A T 1 0 N S 0 A A KEYWORDS, SEVERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC FIM ------------------------------------------------------------------------------------------------------------- GENERAL COMMENT c (II(OISI-:ALi iliS6Ei/6lt6AiS*N6RkAi THE- FO-LLOW-INO- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - BRAIN.113(RI,,COAGULATING OLD ICO). LF. TESTIS (TEO), LIVER (LI). LUNG (LU). PROSTATE (PR). SEMINAL VESICLE IS URET" u -------------------------------------------------------------------------------------------------------------------- o-n INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. p MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CGISOZ SIX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA DATE OF DEATH: 01/25/91 STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 TEITHINAL 6ODY WEIGHT: 409 DATE AND ll"E OF HICROPSIT: 01 2S/91 I:SO PROSECTON-. 14ANCY DIEDRICH RECORDER: MA14CY DIEDRIC POST-FIX WEIGHER: NOT AVAILAG(E PATHOLOGIST: DR. TO" PALMER WEIOHER: PATHOLOGY DEPA ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIOHT ORGAN NAME JORAMS) ---------------------- -------- ORGAN WEIGHT NELATIVE TO BODY WEIGHT (%I -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 pa S TA ...... BRAIN ION) 2.0269 .49SS % 1.0000 WEIOHT LIVER ILII 16.5645 4.OSOO x 8.1735 WEIGHT LUNG Lu 1.4831 .3629 % .?311 WEIGHT LF. IIEITII' FTEOI I.GGSS .4072 X .1210 WEIGHT NY. IESTIS IIEL) 1.6419 .4032 % .1139 WEIGHT ACCESS/SEX ONG ISO) 2.6034 .0365 % 1.2144 WEIGHT ----------------------------------------------------------------------------------------------------------- Ita P A t " 0 L 0 0 Y 0 0 3 E 0 V A T 1 0 N S A & & ORGAN NAME KEYWORDS. SEVERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLO(ITC ft ------------------------------------------------------------ ----------------------------------------------- GENERAL COMMENT .. .. . .. .. .. .. ... ... . . ... . ... . OI(OICSI-:AL@ iliS6Ei/61tdA*NS*N6ltitAi LIVER ILI) iO6Ai . . . . . . . . . . * *INiFIiLTiRATAESi,MO*"O6NU6CL6EAR1 A *CEiLLi * ' * ' ' * * ' * - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN ION$. COAGULATING OLD ICGI, Lf. YESTTS (TEO). LUNG (LU). PROSTATE (FRI. SEMINAL VESICLE ISVI. URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AN[RICA. INC. p MADISON, WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7503 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED FIRST INTERIM $A DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 36 STUDY WEEK OF DEATH: 6 TERNI AL 6ODY WEIGHT: 418 DATE AND TIME Of NECROPSY: 01(24/91 1:19 PROSECTON: JULIE A. LARSEN RECORDER: VAL QUINN POST-FIX WEIG14EN: NOT AVAILAS f PATHOLOGIST: DR. TOM PALMER WEIGHER: DEBBIE FINKEL ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 RG S TA ------ BRAIN (SRI 2.0221 .4121 % 1.0000 WEIGHT LIVETREI (LI) LUNG LUI IS.5999 1.6163 3.7240 % .3859 % 7.7147 .1993 WEIGHT WEIGHT Lf. 11 ITTEIOJI 1.8037 .3120 % .7931 WEIGHT RT. TESTIS 1.6466 .3932 % .1143 WEIGHT ACCESS/SEX 00 SO) 2.8669 .6360 % 1.3119 WEIGHT -----------------------------------------------------------t------------------------------------------------------- a & It P A I H 0 L 0 0 V 0 8 5 E P V A T 1 0 N S * m a OROAN NAME KEYWORDS. SEVERITY.DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Ft ---------------------------------------------------------------------------------------------------------- GENERAL COMMENT Glo'st:-iLi itiS6Ei/6N6AiS'NbR - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNN-E04A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - BRAIN ),,COAGULATING OLD (COI. LF. TESTIS (T[01. LIVER (LIP. LUNG ILUI. PROSTATE IPRI. SEMINAL VESICLE I -------U-R-E-T"-R-i-ew-(-u--------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA H,KZLEION LABORATORIES AMERICA, INC. MADISON. WISCONSIN U,S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C61SO4 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED TERNIIIAL SACRIFI DATE Of DEAT14: 03122191 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TER04INAL 6ODY WEIGHT: 600 DATE AND TIME Of NECROPSY: 03 22/61 8:31 PROSECTOlt-. KEVIIO BILLINGS RECORDER: JOE M. KIZZIE POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: 01. T014 PALMER WEIGHER: JILL BEVAN ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- (GRANSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 pa S IA ------ BRAIN LIVER ILONII 2,1334 21,1053 .3SS4 % 3.SISS N 1.0000 9.8928 WEIGHT WEIGHT LUt4G ILU) Lf. TE I I[ 1.2414 1.8105 .3067 % .3029 X .0631 .8524 WEIGHT WEIGHT AT, TEStTIiSs ITEOI 1.1434 .2911 % .63s$ WEIGHT ACCESS/SEX 0 4 isol 2.6144 .43SS % 1.22SS WEIGHT ------------------------------------------------------------------------------------------------------------ a a a PA IH 0 L 0 0 V 0 8 S E R V A I 10 N S A 0 a ORGA14 NAME KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC Ft ------------------------------------------------------------------------------------------------------------ GENERAL COMMENT GIOWSI: -ALi iliS6Ei/6RdAiS*N6RkAi LIVER ILIJ . ill6R6:'-AlilkAi. *FOCAL - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNN-E04A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN 484). COAOULATING OLD (CO). Lf. TESTIS ITEO), LUNG ILUI. PROSTATE (FRI. SEMINAL VIESICLE ISVI. URETHRA -----------------------------------------7------------------------------------------------------------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. KADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER:C67505 SEX: MALE DOSE GROUP: I SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE DATE OF DEATH: 03/22/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 93 3(22/91 1:22 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: S4$.$ POST-FIX WEIGHER: NOT AVAILA0B E PROSECTOR; RUTH SCHMITZ PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE --------------------------------------------------------------------------------W-E-IG-H-E-R-: --J-I-L-L --BE-V-A-N--------------------- ORGAN NAME --------------------- ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%1 ORGAN TO BRAIN WEIGHT RATIO 0P0 A S TA I -------- -------- -------- -------- BRAIN OR LIVER ILIJ 2.1107 19.6743 .38s7 % 3.5850 X 1.0000 I WEIGHT I LUNG ILU) EF. TESTIS ITEEOli 1.5111 .2755 % 9.2941 .7142 WEIGHT I WEIGHT AT. TESTIS 1.0138 1.6212 .2941 it .2967 % .7624 WEIGHT T ------A-C-CE-S-S-/S-E-X---0-0----S-O)---------------3.-3-3-7-0 -------------------.6-0-8-1 % .7692 l.s76s WEIGHT TI WEIGHT ------------------------------------------------------ ORGAN NAME ; a a P A T H 0 L 0 0 Y 0 8 S E R V A T 10 N S a e h KEYWORDS, EVERITY, DISTRIBUTION ----------------------------------------------------------G--R-O--S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-IC----FI-N-D------- GENERAL COMMENT ac oiosl:-ALi iliS6Ei/6RdAiS*N6Rt4A@ ... .. .. . .. . .. ... .. - -THE- FO-LLO-WING- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - -- -EXA14INATION: - - - - - - - - - - - - - - - BRAIN OR) COAGULATING GLD ICOI. LF. TESTIS ITEO). LIVER (LIJ. LUNG (LU). PROSTATE (PRI, SIE14INALVESICLE lsv UNETHRI (Ui) --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COISOO SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE Of DEATH: STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 TERMINAL 600Y WEIGHT: 441. DATE AND TIME 0 FO'4E'C 4iOPSY: O2tl4/91 8:42 PROSECTON: RUTH SCHMITZ RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAB E PATHOLOGIST: ON. TOM PALMER WEIGHER: VAL QUINN --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R (I ORGAN NAME ---------------------- (GRAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- STA T ------- BRAIN JLOIRJ 2,0323 4004 % 1.0000 WEIGHT LIVER IS.2133 3.* 4466 % 7.4158 WEIGHT LONG (LUI 1.5973 .3019 % .7860 WEIGHT LF. TESTIS TE 1.6141 .3$IS X .1207 WEIGHT RT@ TESTIS TE,0 l@osto .3765 % .8177 WEIGHT ACCESS/SEX 01'a ISO) 3.1538 .714S % l.ssio WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T N 0 L 0 0 Y 0 9 S f P V A T 10 N S a a ORGAN NAME KEYWORDS, ;EV.E%R*ITY. DISTRIBUTION OltOSS FREE-TEXT COMMENTS /* HISTOPATHOLOGIC Ff -----------------------------------------------------7--------------------------------------------------------- GENERAL COMMENT G@'OMCSI-:ALE iliSOEi/i)ltdAiS'NbRkAi - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON.-- - - - - - - - - - - - - - - - BRA COAGULATING OLD (COI, LF. TESTIS (TEOI. LIVER (Lll. LUNG (LUI, PROSTATE (PRI. SEMINAL VESICLE (S URET'H"OVI,R(u,i) -------------------------------------------------------------------------------------------------------------------- tn c@ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67507 DATE Of DEATH: 03/22/91 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE STUDY DAY Of DEATH@ 93 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: S27. DATE AND TIME OF NECROPSY: 03/22/91 8;24 PROSECTOR: KEVIN BILLINGS RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST; DR. TOM PALMER WEIG14ER: JILL BEVAN -------------------------------------------------------------------------------------------------------------------- ORGAN NAME --------------------- ABSOLUTE ORGAN WEIGHT (GRAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 R0 S TA I ------- BRAIN ION) 3.1322 .5943 X 1.0000 WEIGHT LIVER LI) 16.9192 3.2219 % S.4209 WEIGHT LUNG (LU) 1.5115 .2800 x .484S WEIGHT LRFy.. TTEESSTTIISS I(LTEO) 1.9sl2 .3102 % .6229 WEIGHT TEI 1 1.9094 .3023 1 '6096 WEIGHT ACCESS/SEX ONG ISO) 3.1032 Sale % .9907 WEIGHT -------------------------------------------------------------------------------------------------------------------- KEYWORDS. ;E;E;ITYP A I N 0 L 0 6 Y@ 0 8 S E R V A I 1 0 N S 0 11 11 ORGAN NAME . DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FINE -------------------------------------------------------------------------------------------------------------- GENERAL COMMENT (1400CSI:-iL@ il@S6Ei/6N6A'NS'146RI@Ai - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN SRI COAGULATING OLD ICGI. LF. TESTIS ITEOI, LIVER (LIJ, LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE IS -------U-N-E-TH-R-I--J-U-A-I--------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COISOO DATE Of DEATH: 05/16/91 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV STUDY DAY OF DEATH: 148 STUDY WEEK OF DEATH: 22 TE ITHIPIAL DODY WE113HT: S63. DATE AND TIME OF NECROPSY: 8:52 PROSECTOR: GAIL M. NOVAK RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILAOBS(Ele/ol PATHOLOGIST: DR. TOM PALMER WEIGHER: JACK SERFORT -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIOHT RATIO -------- 0 pa S TA T ------- BRAIN LIVER ILBRII LUNG (LU) LF. TESTIS OITIE?ISO) RT. TEST S TE 2.1100 18.9139 1.7534 1.5465 1,6334 .3$69 % 3.3s7l % .3112 X .2745 % .2199 % 1.0000 8.6761 .8043 .7004 .7493 WEIGHT WftGHT WEIGHT WEIGHT WEIGHT ACCESS/SEX 0 3.$IS3 .6772 % i.isoi WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T N 0 L 0 0 Y 0 8 S E R V A t 10 N S 4 A A ORGAN NAME KEYWORDS. *SEV&ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Ft ------------------------------------------------------------------------------------------------------------- GENERAL COMMENT c OAGO.SI.-:A.Li. i.li.S6.Ei/.61tdAiS'M6ltilA@ LF. TESTIS (TEOI MICRO: -SLIGHT, FOCAE -AiR6PhYioiGimiitATiOi . . . . . . THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN (OR). COAGULATING OLD (CO). LIVER (LI). LUNG ILU). PROSTATE (PA). SEMINAL VESICLE (SVI. URETHRA IURI -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR 14ADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C6?509 SEX: MALE DOSE (INOUP: 2 SACRIFICE STATUS: SCHEDULED. FIRST INTERIM SAC DATE Of DEATH: Ot/24/91 STUDY DAY Of DEATH. 36 STUDY WEEK OF DEATH: 8 TERMINAL 13ODY WEIGHT: 464. DATE AND TIME OF NECROPSY: PROSECTOlt: DENNIS HOFFMAN RECORDER: VAL QUINN POST-FIX WEIGHER@ NOT AVAILA0BL1E/24/91 $:So PATHOLOGIST: DR. TOM PALMER WEIGHER: DEBBIE PIRKEL -------------------------------------------------------------------------------------------------------------------- ABSOLUTE 09(IAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 9a O-R-GA-N---NA-M-E------------ JORAMSP -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T ------- BRAIN JOR) 1.9301 .4154 % 1.0000 WEIGHT LIVER L[i LUNG (LU) ll@4500 1.0890 3.7S43 % .3034 s 9.0382 .8741 WEIGHT WEIGHT Lf. TESTIS 0ITEOI 1,1281 .3719 % IOS4 WEIOHT RT@ TEST S TEIISOI 1.1032 .3664 % .8822 WEIGHT ------A-C-CE-S-S-/S-E-X-----Q -------------------2.-8-0-4-1-------------------.6-0-3-3--% --------------1-.4-5-2-4-----------------W--E-I--G-H-T-------- P A T N 0 L 0 0 V' 0 6 S E R V A T 1 0 H S A A A O-R-GA-N---NA-M-E-------------K--E-Y-WO-R-D-S.---;-E-*V-E-R-IT-Y-.--D-IS-T-R-IB-U-T-IO-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-OP-A-T-HO-L-O-GI-C----FI-N------- GENERAL LIVER COMMENT GR(O0S'1: -A.L@ il@S6Ei/blt6A'NS*N'ORMAL (LIJ . . . . . . . . . . . . . . . . . . MIC*OI -MINIMAL, FOCAL .. .. .. li-FiL-ilt-iTi-S*.M-6tt-6N6-C@E-AR'-CiLi- - - - - - - - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE AT MICROSCOPIC EXAMINATION: BRAIN (ON). COAGULATING OLD ICOI. LF. TESTIS (TEO), LUNG (LU). PROSTATE IPPI, SEMINAL VESICLE ISVI, URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLE70N LABORATORIES AMERICA. INC. PR MADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67SIO SEX: MALE DOSE GROUP: 2 SACRIFICE S7A7US: SCHEDULED. FIRST INTERIM SAC DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 34 STUDY WEEK OF DEATH: a TERMINAL BODY WEIGHT: 390. DATE AND 71HE OF NECROPSY: 01(24/91 9:09 PROSECTON: DENNIS WWF04AN RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: DR. TOM PALMER WEIOHER: DEBBIE PIRKIL -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R G O-R-G-AN---NA-M-E -------------- (ONAMS) -------- TO BODY WEIGHT -------- WEI-GH-T---N-A7-1-0 S7A 7 ------- BRAIN JLOIRJ 2.207:04 1.0000 WEIGHT LIVER 14. 9 3:GS.47.. 6.8254 WEIOHT LUNG LLUI LF. TE Ti OITEO 1.6098 1.6477 .4123 % .4221 % .7701 .7812 WEIGHT WEIGHT RT. TESTIS TE 1.6491 .4224 % .7889 WEIO"T ACCESS/SEX 0 (SO) 3.1326 .8024 % 1.4986 WEIGHT ------------------------------------------------------:--------------------------------------------------------------- PA T H 0 L 0 0 V 0 9 S E R V A T 10 H S ORGAN NAME 14EYWORDS, ;EAVEARITY. DISTRIBUTION --------------------------------------------------------- GROSS FREE-YEXY COMENT; &/* HISTOPAY14OLOGIC FIN ------------------------------------------------ GENERAL LIVER COMMENT .1o.cst:-ALE iliS6Ei/61tdAiS*N6*itA@ .. . .. . . (LI) . . . . . I . . . . . . . . . . . . . . . . . . . . MICRO: -SLIOHT, FOCAL -TiFiLiftATiS'.M6N6Nl)CEEAR*CiLE THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN IBR). COAGULATING OLD ICO). LF. TESTIS ITEO), LUNG ILU). PROSTATE IPA). SEMINAL VESICLE ISVI. URETHRA --------------------------------------------------------------- -----------------------@-7 ------------------------- tn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICik, INC. PRI MADISON, WISCONSIN U S A, ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COISII SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACR DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 36 STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: 399.2 DATE AND TIME OF NECROPSY@ /OL 1:23 PROSECTOR: KEVIN BILLINGS RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAIL:I(E24 PATHOLOGIST: DR. TOM PALMER WEIGHER: DEBBIE PINKEL --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OROAN WEIGHT RELATIVE ORGAN TO BRAIN 0R0 A ORGAN NAME --------------------- IGRANSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T -------- BRAIN JLONli .4944 % 1.0000 WEIGHT T LIVER 141.!0143: 3.5734 % 7.2271 WEIGHT 7 LUNG ILUI 1.4519 .3ass % .7391 WEIGHT T LF. TESSTIS ED AT. TE OITTEI 1.6229 1.6192 .40$S % .4058 % .8222 @8207 WEIGHT T WEIGHT T ACCESS/SEX a ISO) 2.9169 .7307 % 1.4?71 WEIGHT T -------------------------------------------------------------- ---------------------------------------------------- P A T " 0 L 0 0 V 0 8 S E R V A T 10 N S A 6 6 ORGAN NAME XEYWOROS. ;EV'ERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPAT"OLOGIC FIND ---------------------- ---------------------------------------------------------------------------------------- GENERAL COMMENT GIGOCSI: -ALi iltS6lEi/bOtdAiS'N6Rt4Ai .. .. .. . .* .. .. .. . - -THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN SRI COAGULATING OLD ICGI, LF. TESTIS ITEOI. LIVER (LIJ. LUNG (LUI, PROSTATE (PRI. SEMINAL VESICLE (SY URET"RI (UAI --------------------------------------------------------------------------------------------------------------------- o-n 4Ln INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C07512 SEX: MALE DOSE (IROUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SAC DATE OF DEAT":0?2413/91 STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: 440.1 DATE AND TIME ECROPSY: 02 13/91 1:00 PROSECTOR: RUTH SCHMITZ RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN ------------------------------------------------------------------------------------------------------------------ ORGAN NAME ------------------- ABSOLUTE ORGAN WEIGHT -(-G-R-A-MS-)-- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 R0 A S TA T ------ BRAIN ILBlfA LIVER LUNG (LU) LF. TESTIS OITEOISOI 2,0451 ls.lsoo 1.680S I.SS62 .4047 Z 3.40is x .3118 X .3536 X 1.0000 7.7SO2 .8217 .7609 WEIGHT T WEIGHT I WEIGHT t WEIGHT T Rt. TESTIS TEI 1.4831 .3370 % .72S2 WEIGHT T ACCESS/SEX a 3.0879 .0911 % 1.5001 WEIGHT I --------------------------------------------------------------------------------------------------------------------- KEYWORDS. ; ORGAN NAME EVERITY. DISTRIBUTION GROSS FitEE-TEXT COMMENTS HISTOPATHOLOGIC FIND ------------------------------------------------------------------------------------------------------------- MANDIRICERVLM . 4R""OIS:.-iAi.Gi. . . . . . . . . . . . . ..... -.LE.F.T:.-UT.OP-1-0X-It X*S*t*@ iF6R'C6f4OAili0iild"i MICRO: -MODERATE, MULTI-FOCAL 6 X 4 X 2 MR) (CAP 6 -HYPERPLASIA. LYMO"ORITICULAR CELL THE- FO-LLO-WINO- T-ISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - BRAIN (OR). COAGULATING OLD (CO). LF. TESTIS ITEO), LIVER (LI), LUNG (LU). PROSTATE IPRI, SEMINAL VESICLE ISV URETHRA JUR) --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67513 SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE OF DEATH: 05/17/91 STUDY DAY Of DEATH: 149 STUDY WEEK Of DEAT14: 22 TERMINAL 6ODY WEIGHT: 680.3 DATE AND TIME OF NECROPSY: OS(17/91 1:07 PROSECTOR: PUT" SCHNITZ RECORDER: JOE N. KIZZIEE POST-FIX WEIOHER: NOT AVAILAS t PATHOLOGIST: DR. TOM PALMER WEIGHER: JACK SERFORT --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- -(-G-R-A-MS-)-- OROAN WEIGHT RELATIVE TO BODY WEIOHT -------- OROAN TO BRAIN WEIGHT RATIO -------- 0 Ra A STA T -------- BRAIN JOR) 2.29S? .3375 % 1,0000 WEIOHT T LIVER TLIJ 21.4360 3.1511 % 9.3371 WEIGHT TI LUNG LUI 1.9058 .2801 .8302 WEIGHT LF. TEI ITE 1.6522 @2429 .7197 WEIGHT TI RT. TEST1I1S TEI0I 1.7142 .2520 X .7467 WEIGHT ACCESS/SEK 0 0 SO) 3.9260 .51?1 % t.7102 WEIGHT T --------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S 0 * & ORGAN NAME KEYWORDS, ;E*VERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC FIND -------------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LI) -- -- -- GI(OICSI-:4L@ itiS6Ei/61tdAiS'N6RAA@ . .. .. .. .. . ... . .. .. .. . .. .. . .. .. .. .. .. . ... .. .. MICRO: -MINIMAL, FOCAL -INFILTRATES.MONONUCLEAR CELL - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - THE FOLLOWING TISSUES WERE UNNE14ARKABLE AT MICROSCOPIC EXAMINATION: BRAIN JOR). COAGULATING OLD (CO). LF. TESTIS (TEOI. LUNG ILU). PROSTATE (PRI, SEMINAL VESICLE (SVI, URETHRA I --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C07514 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACO DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 36 STUDY WEEK OF DEATH; 6 TERMINAL 600Y WEI(INT: 440. DATE AND TIME OF NECROPSY: (24/91 7:03 PROSECTOR; JULIE A. LARSEN RECORDER: VAL QUINN POST-FIX WEIGHER: H07 AVAILAoSl E PATHOLOGIST: OR. TOM PALMER WEIGHER-. DEBBIE PIRKEL ----------------------------------------------------------- -------------------------------------------------------- ORGAN NAME ---------------------- ABSOLUTE ORGAN WEIGHT JORAMSP -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Ra STA I ------- BRAIN JLONIJ 2.1070 4786 % 1.0000 WEIGHT LIVER 16.1093 3.*6132 % 7,6741 WEIGHT LUNG (LU) 1.5906 .3613 % .75 9 WEIGHT Lf. TESTIS ITEO) 1,6031 .4096 % .$se4 t WEIGHT RT. TESTIS ITEI 1.7679 .4016 % .8391 WEIGHT ACCESS ISO) 2.6622 .6061 % 1.2664 WEIGHT -------------- ------------------------------------------------------------------------------------------------------- a A & P A I H 0 L 0 0 Y. 0 8 S E R V A I 1 0 N S 0 ok & ORGAN NAME ---------------------- K-E-Y-W-O-R-D-S-.--S-EV-E-R-I-T-Y-,--D-I-S-T-R-I-B-UT-I-O-N----------G-R-O-SS---F-R-EE---T-EX-T----CO-M-M-EN-T-S----/---H-IS-T-O-P-AT-H-O-LO-G-I-C----F-I-M --- GENERAL COMMENT GI(OICSI-:iLi iliS6Ei/6ft6AiS'ti6ftkA@ LIVER (LI) - -714-E FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-A141-HATI-ON:- - - - - - - - - - - - - - - BRAIN JOR). COAGULATING GLD (CO). LF. TESTIS ITEO), LUNG ILUI. PROSTATE IPRI, SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67515 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED. FIRST INTERIM SAC DATE OF DEATH: 01424/91 STUDY DAY OF DEATH: 36 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: 396@ DATE AND TIME OF ECROPSY: /24/91 7:16 PROSECTOR: JULIE A. LARSEN RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILAoBtLE PATHOLOGIST-. ON. TOM PALMER WEIGHER: DER13TE PINKEL -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- -(-G-R-A-MS-)-- OR"N WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIO"T RATIO -------- 0 R0 S TA T ------- BRAIN JLORli 141.9549 .4937 % 1.0000 WEIGHT LIVER .9406 3.7729 % 7.0420 WEIGHT LUNG ILU) 1.4892 .3761 % .7611 WEIGHT LF. T I E t.s2o6 .40LY % .1136 WEIGHT NT, TEESSTTISS j TIE101 1.5946 .4021 % Ilsy WEIGHT ACCESS/SEX a SO) 2.2112 .5544 x 1.1311 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A I N 0 L 0 0 V, 0 8 S E R V A T 1 0 N S A a sk ORGAN NAME KEYWORDS, ;EOVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIN ------------------------------------------------------------------------------------------------------------- GENERAL LIVER COMMENT GAOOCSI:-iL@ itiS6Ei/6lt6AkS'N6ltkA@ ILI) . . . . . MICRO: -i4lklk@.'M6Lil:F6C@L . . . . . . . . . . -.liFiLiRiTiS'M6N6N6CiEAR'CiL@ - -THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-A14-1HAT-ION:- - - - - - - - - - - - - - - BRAIN JOR), COAGULATING OLD ICO), LF. TESTIS (TEO). LUNG ILU). PROSTATE IPRI. SEMINAL VESICLE ISVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C6?Sl$ SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED. FIRST POST-RECOV DATE Of DEATH: 05/16/91 STUDY DAY OF DEATH: 148 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: $71.1 DATE AND TIME Of NECROPSY: 05(16/91 8;17 PROSECTON: JULIE A. LARSEN RECORDER: JOE N. KIZZIEE POST-FIX WEIOHER; NOT AVAILAB E PATHOLOGIST: DR. TOM PALMER WEIGHER: JACK SERFORY --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OROAN NAME ---------------------- (GRAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- ORGAN 70 BRAIN WEIGHT RATIO -------- 0N0 A S TA T -------- BRAIN JORI 2.2907 .337S % 1.0000 WEIGHT T LIVER (Lll 19.8095 2,9113 % 8.6478 WEIGHT I LUNG (LU) 1.9520 .2170 % .1521 WEIGHT T LF. TESTIS I-TE .277S % .8223 WEIGHT 7 RT. TESTIS ITEO11 1'..:"3167 .2701 % .8022 WEI(IHT T ACCESS/SEX ORO ISO) 3.2904 .4847 % 1.4364 WEIGHT I --------------------------------------------------------------------------------------------------------------------- V P A 7 H 0 L 0 0 V - 0 9 S t R V A 7 1 0 N S & 1%A ORGAN NAME KEYWORDS, ;EVIE';IT . DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC FIND -------------------------------------------------------------------------------------------------------------- GENERAL COMMENT GR"OSCl: -AL@ iliS6Ei/6R6A4S'N61ti4Ai .. . ... .. . .. THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT^ MICROSCOPIC EXAMINATION: BRAIN 13*1 COAGULATING GLD (CGI, Lf. TESTIS ITEO). LIVER (LI). LUNG ILUI. PROSTATE (PR), SEMINAL VESICLE (SV URE7HRI JOA) --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL MU14SER: C67517 SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV DATE OF DEATH: 05/17/91 STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: sit. DATE AND TIME OF NECROPSY: 05/17/91 8:33 PROSECTON: JULIE A. LARSEN RECORDER: JOE M. KIZZIEE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. 7004 PALMER WEIGHER: DENNIS HOFF14AN -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGA" TO BRAIN 0 0 (1 ORGAN NAME -------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA T ------- BRAIN (OR) 2.2742 .30?S 1 1.0000 WEIGHT LIVER ILI) 18.9402 3.0601 % 6.3263 WEIGHT LUNG ILUP 1.6309 .2645 % .7198 WEIGHT LF. T[SSIII 17IEO Nt. T El 2.0113 2.1674 .3250 % .3SO3 % .2244 .9530 WEIGHT WEIGHT ACCESS/SEX 0 0 ISO) 4.2763 .6911 % 1.8804 WEIGHT ------------------------------------------------------------------------------------------------------------------- P A I H 0 L 0 0 Y' 0 8 S IE N V A T 1 0 N S a a a ORGAN "AM[ ---------------------- KEYWORDS. ;E*VER6ITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOTC FIN --------------------------------------------------------------------------------------- OENERAL LIVER COMMENT (LIP G@'30(S:L-:ALi iliS6Ei/6R6AiS*N6RAAi ..... .. .. . .. .. .. .. .. .. . .. .. .. .. .. ... . .. .. .. . .. .. . MICRO: -MINIMAL. FOCAL -INFILTRATES.1401#ONVCLEAR CELL - -THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-A141-HATI-ON:- - - - - - - - BRAIN JOR), COAGULATING OLD ICO). LF. TESTIS (TEO). LUNG (LU). PROSTATE (POP, SEMINAL VESICLE ISVI. URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PA MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?Stl SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF DEATH: 03426/91 STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: 467. DATE AND TIME OF ECROPSY: PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAI LA036(E2$/Ol 0:04 PATHOLOGIST: ---------------------------------------------- ------ ON. TOM PALMER ----- ---------- WEIGHER: JOE N. KIZZIEE --------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0A a ORGAN NAME -------------------- (GRAMSI -------- TO BODY WEIGHT (7L) -------- WEIGHT RATIO -------- S TA T ------ BRAIN 2.0016 .4299 % 1.0000 WEIGHT LIVER IOLRII 14.0143 2.9096 % 6.9171 WEIGHT LUNG ILU11 1.3711 .29SO % .6161 WEIGHT LF. TE T 'ITEOI 1.6312 .3491 % .8121 WEIGHT AT. TESTIS O(TEII 1.7067 .36s3 it .1497 WEIOHT -------AC-C-E-S-S/-S--X---R-O---IS-O-)--------------3-'-1-43-9-------------------.-07-2-9--% ---------------I-.--S-O-S2-------------W-EI-G-HT------ PA IH 0 L00 V 0 0 SE RV A TI* " S ORGAN NAME KEYWORDS, ;E;E;ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS* */* HISTOPAT"OLOGIC Fl ------------------------------------------------------------ -------------------------------------------- GENERAL COMMENT Jac GROSI: -ALi itiS@Ei/6it6AiS'N6ftilAi --- -- -------- --- --- --- --- --- --- --- ----THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BRI. COAGULATING OLD (CO). LF. TESTIS (TEOI. LIVER ILII. LUNG ILU), PROSTATE (PRI. SEMINAL VESICLE IS URETHRA JUN) --------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON, WISCONSIN U.S,A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C87SIO SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF DEATH: of03426/91 STUDY DAY Of DEATH; 97 STUDY WEEK OF DEATH: 14 TERMINAL 600Y WEIGHT: SIS. DATE AND TIME ECROPSY: 03(26/91 8:43 PROSECTOR: DEBBIE PINKEL RECORDER: JILL BEVAN POS7-FIX WEIGHER: N07 AVAILAS E PATHOLOGIST: DR. T014 PALMER WEIGHER: JOE 11. KIZZIEE -------------------------------------------------------------------------------------------------------------------- ORGAN MA14E ---------------------- ABSOLUTE ORGAN WEIGHT (GRANSO -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0a a STA T ------- BRAIN IDR) 2,1131 .4100 % 1.0000 WEIGHT LIVER IL]i 16.7043 3.2410 % 7.90si WEIGHT LUNG (LU) 1.0032 .3111 % .7517 WEIGHT LF. 71STIS TEO 1.1623 .22SS % ssoo WEIGHT RT, TESTIS TEIISO) 1.8261 .3544 X .864S WEIGHT -------A-CC-E-SS-/-S-EX----0-1-0---------------------3-.-04-*-1---------------7--------.-5-9-1-4 --% ---------------1-.-44-2-5--------------W-EI-G-HT------ P A T " 0 L 0 0 Y 0 9 S E A V A 'r1 0 N S a & a ORGAN NAME KEYWORDS. ;E*VE*RITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl ---------------------- --------------------------------------------------------------------------------------- LF. TESTIS ITEO) GROSS: MICRO: -iMiL@ -MODERATE, -MODERATE. DIFFUSE FOCAL -ii i iO*t4@ lf*ON C*OM*PAR'I'SO'NRIGHT' *IS'2"S'X IS'X'10' :ATROPHY/DEGENERATION INFLAMMATION, GRAMULOMAYOUS LIVER (LI) .... ... . .. .. .. .. .. . .. .. . .. .. .. .. .. .. . .. .. . .. ... . . MICRO: -MINIMAL. FOCAL -INFILTRAYES.MDNONUCLEAR CELL THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMARK-ABL-E - AT- MI-CROS-COPI-C - EX-A141-HATI-ON:- - - - - - - - BRAIN (DR), COAGULATING GLD (CO), LUNG ILUI, PROSTATE (PR). SEMINAL VESICLE ISVI, URETHRA (URI -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. p MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67S20 DATE OF DEATH: OS/17/91 SEX: MALE DOSE OROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST POST-RECO SYUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: SSI DATE AND TIME OF NECROPSY: 8:02 PROSECTOR: JULIE A. LARSEN RECORDER: JOE N. KIZZIE POS7-FIX WEIGHER: NOY AVAIL250(Ely/ol PATHOLOGIST: DR. YOt4 PALMER WEIGHER: JACK SERFORT ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- JORAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Aa S--T--A-- BRAIN 2.2930 4141 % 1.0000 WEIGHT LIVER ILBRII IS.8940 2'.84so x 6.8399 WEIOHT LUNO LU i.essi .3009 x .7231 WEIGHT Lf. Yfilli I' 1.72S$ .3132 x .7526 WEIGHT PT. TESTIS ITEEDI 1.7011 .3099 % .7449 WEIGHT ACCESS/SEX ORO ISO) 4.2444 .7702 % 1.9sio WEIGHT ------------------------------------------------------------------------------------------------------------------- P A T N 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S A A h ORGAN NAME KEYWORDS. *SEV*ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPAT"OLOQIC Fl ----------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LI) 0100CSI: -iLi iliS6Eili)lt6AiS*N6RilAi MICRO: -iLiG*"T*.iO@Ai . . . . . . -SLIGHT. MULTI-FOCAL -NiCiOili.6OiG6LiTiVi -HYPERPLASIA,BILE DUCT .. .. .. THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON;- - - - - - BRAIN IOR). COAGULA71NO OLD ICO). Lf. TESTIS 17EO), LUNG ILU). PROSTATE IPR). SEMINAL VESICLE ISV). URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. p MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C67521 DATE OF DEATH: 03/22/91 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFI STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: SS? DATE AND TIME OF NECROPSY: 03/22/91 8:43 PROSECTOR: KEVIN BILLINGS RECORDER: JOE N. KIZZIE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra O-R-GA-N---NA-M-E -------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIOHT RATIO ------- S--T--A-- BRAIG IDR) 2.2002 .3940 Ix 1,0000 WEIOHT LIVE (Lll 16.9153 3.0461 % 7.7199 WEIGHT LUNG (LUI 1.7320 .3106 % .7172 WEIGHT LF. TESTIS (TEO) I.S$41 .2141 % .7200 WEIGHT RY. TESTIS O@TEIISOI 1.6421 .2945 % .7463 WEIGHT ACCESS/SEX 0 3.4940 ---------------------------------------------- ---- ----- - - .6266 % 1.5810 WEIGHT - ---------- --- - - -- ---------------------------- a 0 * P A T H 0 L0 0 00S ER VA T 10NS 6 * 6 O-R-GA-N---NA-M-E -------------K--E-Y-WO-R-D-S,---S-E-VE-R--IT-Y-.--D-I-S-T-RI-B-U-TI-O-N----------O--N-O-SS---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---"I-S-T-O-PA-T-H-OL-O-G-IC----Fl------ GENERAL CONP4ENT 0400CSI:-ALi iliS6Ei/6R6AiS'146R'MA*L . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN,,IBII,,COAGULATING OLD (CGI, LF. TESTIS (TEO). LIVER (LI). LUNG (LU). PROSTATE (PR). SEMINAL VESICLE -------U-RE-T------u---------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CO?522 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: S2S. DATE Of DEATH: 024ic2491 DATE AND TIME OF OPSY: PROSECTON: RUTH SCHMITZ RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILA0B2(I12/91 7:49 PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY DEPAR ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELA71VE ORGAN TO GRAIN 0 RG ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA T ------- BRAIN ILSl*f 2.0794 .39SA % 1.0000 WEIGHT LIVER LUNG (LUI ITE01 21.9998 I.S$73 1.7694 4.1833 % .3011 % .3365 % lo.s799 .7633 IS09 WEIGHT WEIGHT WEIGHT LF. TESTIS RT. TESTIS E 1.7859 .3390 % .1519 WEIGHT ACCESS/SEX 040T 'ISO, 3.3206 .6314 % l.s969 WEIGHT ------------------------------------------------------------------------------------------------------------------- KEYWORDS. ; O-R-GA-N--N-A-M-E ------------------------E-VE-R-I-TY-.---DI-S-T-R-IB-U-T-IO-N----------G--R-O-S--S---FR-E-E--T-E-XT---C-O-MM-E-NT-S-------HI-S-T-OP-A-T-HO-L-O-GI-C----FI-N------- GENERAL COMMENT GI'OOCSI-:ALi iliS6Ei/i)it6AkS"N61tt@Ai. . . . .. - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - - BRA COAGULA71NO (ILD (C(ii,Lf. TESTIS ITEO), LIVER (LI). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (S -------U-R-E-T'H-"R-I-"-lu-,-k-l--------------------------------------------------------------------------------------------------- Cp% INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANT14AL NUMBER: C67S23 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED. TER14INAL SACRIFIC DATE OF DIA7H: of03/26i9l STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 613. DATE AND TIME NEC OPSY: 03 26191 8:32 PROSECTON: DEBBIE PIRKEL RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST-. DR. TOM PAL14ER WEIGHER: JOE M. KIZZIEE ------------------------------------------------------------------------------------------------------------------- ORGAN NAME ---------------------- ABSOLUTE ORGAN WEIGHT (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) -------- ORGAN TO GRAIN WEIGHT RATIO -------- 0 Ra SIA I ------- BRAIN 1L8li 2.1718 35SI % 1.0000 WEIGHT LIVER 2o.83s3 3:39SG % O.S$27 WEIGHT LUNG ILUI 1.7937 .2923 % @1233 WEIGHT LF. If It OITI'L 1.8941 .3087 % .8603 WEIGHT RT. TESTIS TEL I.IS79 .3021 % .1527 WEIGHT ACCESS/SEX a ISO) 3.2076 S228 % 1.4722 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A I N 0 L 0 0 Y' 0 9 S E R V A I 1 0 N S a a * ORGAN NAME ---------------------- KEYWORDS, ;E*VE*RITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC FIM --------------------------------------------------------------------------------------- GENERAL COMMENT GIGOCSI: -iLi iliS6Ei/6N6A4S'%60tkAi - -THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-A14I-NAT-ION:- - - - - - - - - - - - - - - BRAIN OR) COAGULATING OLD (COP, LF. TESTIS ITEOI. LIVER (LIJ. LUNG (LU). PROSTATE (PO). SEMINAL VESICLE (S UNEINRI IU*l ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?524 SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACR DATE OF DEATH: OF01423/91 STUDY DAY OF DEATH: 3S STUDY WEEK OF DEATH: S TERMINAL 6ODY WEIGHT: 376.6 DATE AND TIME ECROPSY: 01(23/91 7:10 PROSECTOR: DENF41S HOFFMAN RECORDER: JILL BEVAN POST-FIX WEIOHER: NOY AVAIL AB E PATHOLOGIST: DR. TOM PALMER WEIGHER: GAIL M. NOVAK --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OROAN WEIGHT RELATIVE ORGAN 10 BRAIN 0R0 A OROAN NAME --------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIO"T RATIO -------- S tA T -------- BRAIN R 2.0308 S392 % 1.0000 WEIGHT T LIVER IOLII is.so7i 4.1973 % 7.7844 WEIGHT 7 LUNG Lo 1.3115 .3sol % .6493 WEIGHT 7 LF. TELLTII (TEOI 1.3102 .3479 % .64s2 WEIGHT 7 PT. TESTIS ITEI 1.3303 .3532 % Gssl WEIGHT T ACCESS/SEX ORO ISOI 2.5370 .6737 % 1.2494 WEIGHT 7 --------------------------------------------------------------------------------------------------------------------- KEYWORDS, ; ORGAN MAKE [VERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS "ISTOPATHOLOOIC FIND -------------------------------------------------------------------------------------------------------------- GENERAL COMMENT OIGCI: os -ALL il@S6E@/61tdAiS'N6RitAL . .. ... THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MICROSCOPIC EXAMINATION: BRAIN IDA), COAGULATING OLD (CGI. LF. TESTIS (TEO). LIVER (LIJ, LUNG (LU), PROSTATE (PR). SEMINAL VESICLE ISV UREIHR JUR) --------------------------------------------------------------------------------------------------------------------- OD INDIVIDUAL APPENDIX D ANIMAL PATMOL043Y DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON, WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUM13ER: C6752S SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED. FIRST INTERIM SAC DATE Of DEATH: 01/23/91 STUDY DAY OF DEATH: 3S STUDY WEEK OF DEATH: 5 TERMINAL BODY WEIGHT: 434. DATE AND TIME OF NECROPSY: (23/91 7:22 PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-OT--A-V-AI-L-A-oOl--E----------------PA-T-H-OL-O-G-IS-T-:---D-R.---TO-M--P--A-L--M-E-R------------------W-EI-G-H-E-R:---G-A-IL--M-.--N-OV-A-K-------- ABSOLUTE DOTGAM WEIGHT DROAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ORGAN NAME ----------------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO ----7 --- S TA T ------- BRAIN JLORli 1.1931 .43$S % 1.0000 WEIGHT LIVER LUNG ILU) 17.111S 1.5590 4.0990 % .3586 % 9.4123 .823S WEIGHT WEIGHT LF. TESTIS (TEO) 1.8906 .4349 % .9917 WEIGHT RT. TESTIES OitEllSol ACCESS/S X 0 1.8599 2.7211 .4279 % .62$0 % .0825 1.4374 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- KEYWORDS, ; O-R-OA-N---NA-M-E -----------------------E-VE-R-I-TY-.--D-I-S-T-RI-B-U-TI-O-N----------G-R--O-S--S--F-R-E-E--T-EX-T---C-O-MM-E-NT-S-------"I-S-T-OP-A-T-HO-L-O-GI-C---F-I-N------- GENERAL COMMENT G@OOCSI: -AL@ tTiS6Ei/6lt6AiS*W6Ri4Ai - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-At4l-NAT-IO14-: - - - - - - - - - - - - - - - BRAIN 1,,COAGULATING OLD (CGI. LF. TESTIS ITEO). LIVER ILI), LUNG (LU), PROSTATE (PA). SEMINAL VESICLE (S -------U-R-ET-H-RI-O-Rt-u----------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. p MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67526 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFI( DATE OF DEATH: 03/25/01 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: 513 DATE AND TIME OF NECROPSY: 03(25/91 10:07 PROSECTOR: JACK SERFORT RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: DR. TOM PALMER WEIGHER: STEVE VAN ADES ------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE 70 BODY WEIGHT ORGAN TO BRAIN WEIGHT RATIO 0 Ra S TA ---------------------- -------- -------- -------- ------ BRAIN 1L8IJ 2.IS9$ .4201 % 1.0000 WEIGHT LIVER LUNG ILUI 'o 15.4771 1,5033 3.0158 % .2929 % 7.1600 .6960 WEIGHT WEIGHT LF. TE Ti OITEIISO. 3 .3311 % .7860 WEIGHT RT. TESTIS TE '1.7629?94 .3366 % .7998 WEIGHT ------A-C-CE-S-S-/S-E-X----(-1-------------------2.-9-0-3-0-------------------.5-6-5-7--% --------------1.-3-4-4-1------------W-EI-G-HT------------------ y P A I N 0 L 0 0 Y- 0 8 S E R V A I 1 0 N S A ftA ORGAN NAME KEYWORDS. S'E.V0E.RI*T . DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Ff ------------------------------------------------------------------------------------------------------------- GENERAL COMMENT GIOGCSI:-ALi iliS6Ei/6R6AiS*N61ti4A@ - -THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN IBRI, COAGULATING GLO ICO). LF. TESTIS ITEO). LIVER (LI). LUNG ILU). PROSTATE IPRI, SEMINAL VESICLE IS URETHR JUR) -------------------------------------------------------------------------------------------------------------------- 14 INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLE70H LABORATORIES AMERICA. INC. Pal MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7527 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: DATE OF DEATH: 02413VI STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I DATE AND TIME OF IC OPSV' 02(13/91 1:51 PROSECTON: RUTH SCHNITZ SCHEDULED SECOND INTERIM SAC TERMINAL 6ODY WEIGHT: 495.1 RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILAB E PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- -J-O-R-AM-S-)-- OROAN WEIGHT RELATIVE TO BODY WEIGHT IX) -------- ORGAN TO BRAIN WEIGHT RA710 -------- 0R 0A S 7A 7 -------- BRAIN JLOIRJ 2.2223 .4419 % 1.0000 WEIGHT 7I LIVER 16.3032 3.2929 % 7.3362 WEIGHT LUNG (LU) 1.7484 .3531 % .7808 WEIGHT 7 EF. TESTIS ol@TI0IIS.. I.IOS4 .3026 % .1079 WEIOHT T RT. TEST S IE 1.8030 .3642 % .8113 WEIONT I ACCESS/SEX 0 3.2105 .6415 % 1.4447 WEIO"T T --------------------------------------------------------------------------------------------------------------------- ; a it P A T H 0 L 0 0 Y, 0 0 S E N V A T 1 0 N S a 0. k ORGAN NAME KEYWORDS, EVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPA7HOLOGIC FIND ------------------------------------------------------------------------------------------------------------- GENERAL COMMENT 0100CSI:-ALi iliS6Ei/6R6AiS'N6RkA@ . . . .. .. . .* * . .. ... . - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN JOR) COAGULATING OLD ICO). LF. TESTIS (TEO), LIVER ILII. LUNG ILU). PROSTATE IPR). SEMINAL VESICLE ISV URETHO (ui) --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANTMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CB?528 DATE OF DEATH: 05/17/91 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: Sg?.l DATE AND TIME OF NECROPSY: 05 17/91 7:30 PROSECTON: JULIE A. LARSEN RECORDER: JOE H. KIZZIEE POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PAL14EN WEIGHER: JACK SERFOR7 --------------------------------------------------------------------------------------------------------------------- ABSOLUTE OROAR WEIGHT ORGAN WEIO"7 RELATIVE ORGAN TO BRAIN 0A 0A ORGAN NAME ---------------------- JORAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T -------I- BRAIN 11L3lRi 2.2437 3952 % 1.0000 WEIGHT LIVER 17.2708 3.'0417 % 7.6274 WEIGHT 7 LUNO (LUI 1.5680 .2702 % .6981 WEIGHT T LF. TESTIS I-TEOI 1.7532 .310S % .7858 WEIGHT 7 OT. TESTIS OITEIISOI 1.7366 .30SO % .7740 WEIGHT I ACCESS/SEX a 3.6242 .6383 % 1.6153 WEIOHT 7 --------------------------------------------------------------------------------------------------------------------- KEYWORDS. ; ORGAN NAME EVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS "ISTOPATHOLOGIC FIND ---------------------- ---------------------------------------------------------------------------------------- GENERAL COMMENT 0400CSI: -AL@ itiSOEi/6R6AkS*N6Ri4Ai LIVER (LI) . i4l6Pt6:'-AthIkA@, 'liFiLiltA7iS'M6N6N6CifAlt'CiLi --- -- -- ------ ---- --- -- ---- --- --- -- ----- THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (ON), COAGULATINO OLD ICO). LF. TESTIS (TEO). LUNG ILU). PROSTATE IPRI. SEMINAL VESICLE (SVI. URETHRA I -------------------------------------------------------------------------------------------------------------------- r,3 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PRI MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMALNUMBER:C67S29 DATE Of DEATH: 01/24/21 DATE AND TIME OF NECROPSY: SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACO STUDY DAY OF DEAT14: 36 STUDY WEEK OF DEAT14: 6 IfRHINAL ADDY WEIGHT: 404. 1(24/01 8:47 PROSECTON: KEVIN BILLINGS RECORDER: VAL QUINN POSI-FIX WEIGHER: NOT AVAILA0S E PATHOLOOIST: DO. 7014 PALMER WEIGHER: DEBBIE PINKEL -------------------------------------------------------------------------------------------------------------------- ORGAN NAME ------------- ABSOLUTE ORGAN WEIO"T JORAMS) -------- OROAN WEIOHT RELATIVE TO BODY WEIGHT 1%) -------- OROAN TO BRAIN WEI-(-I-"T--R-AT-I-O S0IAAa I ------- BRAIN JLORli LIVER 2.1694 14.7411 S363 % 3.'6443 % 1.0000 9,79so WEIGHT WEIGHT 7 LUNG LLUI LF. TE TIS JTEO11@,'o, 1.4026 1.76s2 .3407 % .4364 % .640S .8137 WEIGHT WEIGHT NT. YESTI TE 1.7033 .4211 % .7851 WEIGHT ACCESS/SEX 0 2.S4S6 .6293 % 1.1734 WEIGHT I -------------------------------------------------------------------------------------------------------------------- P A I n 0 L 0 0 Y' 0 9 S E N V A I 1 0 N S e & 1% ORGAN NAME KEYWORDS. S*EVAER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FIN[ ------------------------------------------------------------------------------------------------------------- GENERAL COMMENT c 04(olst:-ALi iliS6Ei/61tdAiS*N&i4Ai . . .. .. . THE- FO-LLO-WINO- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COPIC EXA14INATION: BRAIN Be) COAGULATING OLD ICO), LF. TESTIS ITEOI. LIVER (LI). LUNG ILUI, PROSTATE (PRI. SEMINAL VESICLE (S% UREIHRI IU*l --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PA MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67S2O DATE OF DEATH: 014i2isi SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC STUDY DAY OF DEATH@ 35 STUDY WEEK OF DEATH: S TERM114AL ADDY WEIGHT: 422. DATE AND TIME OF OPSY: (23/01 9:20 PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAoSi f PATHOLOGIST: DR. TOM PALMER WEIGHER: GAIL M. NOVAK - ---------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- (GRAMS) -------- DROAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Ra S--T--A--I- BRAIN 1L0li LIVER LUNG (LU) 2,1181 14.7178 -1.SS 1? 5007 3.4327 % .3623 1 1.0000 6.9SS2 .7366 WEIGHT WEIGHT WEIGHT LF. TES IS IYTEEIIDI l.s79s .373$ % .1464 WEIGHT AT. TESITS l.soso .3774 % .7537 WEIGHT ACCESS/SEX 0 0 Sol 2.6398 .6247 % 1.241S WEIGHT -------------------------------------------------------------------------------------------------------------------- P A 7 H 0 L 0 0 V' 0 8 S E P V A I 1 0 M S A I I ORGAN NAME KEYWORDS, *SE;ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIN ------------------------------------------------------------------------------------------------------------- OEMENAL COMMENT GI(OICSI-:ALi iliS6lEi/6R6AiS*t#6fliiAi LIVER (LI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141COO: -MINIMAL, FOCAL -INFILTRATES,MOMO"UCLEAR CELL THE FOLLOWING TISSUES WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-A14-1HAT-ION:- - - - - - - BRAIN (OR), COAGULATING OLD (CO). LF. TESTIS (TEO), LUNG (LUI. PROSTATE (PRI. SEMINAL VESICLE ISV), URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PRI MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- AN104AL NUMBER: C07531 DATE Of DEATH: 02/12/91 SIX: MALE STUDY DAY OF DEATH: DOSE 55 GROUP: 2 SACRIFICE STATUS: STUDY WEEK OF DEATH: I SCHEDULED, SECOND INTERIM SAC TERMINAL BODY WEIGHT: S24.$ DATE AND TIME OF NECROPSY: 02(12/91 8:2S PROSECTON: KEVIN BILLINGS PATHOLOGIST: DR. YON PAL14EN RECORDER: DENNIS HOFFMAN WEIGHER: PATHOLOGY DEPAR P-O-S-T--F-I-X --W-EI-G-H-ER-:---N-OT--A-V-AI-L-A-O--E--------------------------------------------------------------------------------- ABSOLUTE 0110AN WEIGHT ORGAN WEIGHT RELATIVE ORGAN 10 BRAIN 0 aG ORGAN NAME (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T ------- ---------------------- BRAIN 2.0324 3173 % 1.0000 WEIGHT LIVER (IOLRII LUNG LUI 19.1474 1.5564 3'7819 % .2280 % 9.?$SS .7658 WEIGHT WEIGHT LF. TEITI OITEOJISOP 1.5803 .3023 % .?SOS WEIGHT RT, TESTIS TE 1.5311 .2917 % .7533 WEIGHT ------A-C-C-ES-S-/S-E-X-----a-------------------2-.-73-8-7--------------------------S--21--2--%--------------1-.-3-4?-S-------------WE-I-GH-T--------- a A PA T N 0 L 0 0 Y 0 9 S E R V A T 10 N S # & A O-R-GA-N--N-A-M-E -------------K--E-Y-WO-R-D-S,---;-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-OP-A-T-"O-L-O-GI-C---F-I-N------- GENERAL COMMENT . . . . .*. . I . . . . . . . . . . . . . . . . . . . . . . . . G@(OICSI-:ALL iliS6fi/6R6AiS'N6Ri4Ai - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-A14-INA7-1ON:- - - - - - - - - - - - - - - BRAIN JOR), COAGULATING GLD ICGI, LF. TESTIS ITEO). LIVER (LIP. LUNG ILU), PROSTATE IPRI. SE14INAL VESICLE IS URETHR (Un I INDIVIDUAL APPENDIX D AN104AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCOHSI" U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COYS32 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE OF DEATH: OF02412/91 STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: 414. DATE AND TIME CROPSY: 02 12/91 9!44 PROSECTON: KEVIN IIILLINGS RECORDER: DENNIS 14OFFMAN POST-FIX WEIGHER: NOT AVAILAB(C PATHOLOGIST: 00. TOM PALMER WEIGHER. PATHOLOGY DEPAR --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- IGRANSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (Z) -------- ORGAN TO BRAIN WEIGHT RATIO ----- 0 Ra S TA I BRAIN JLONli 2.0003 .4131 % 1.0000 @ilGHT LIVER 17,2673 3.5868 % 1.6823 WEIGHT LUNG ILUI LF. TE It It 1.3694 1.7019 .2121 % .3515 % .0840 .1508 WEIGHT WEIGHT *T. TESTI TE,0 1 7102 .3532 % IS50 WEIGHT ACCESS/SEX S OIG isol 3.0?S? .6352 % I.S376 WEIGHT -------------------------------------------------------------------------------------------------------------------- V P A T N 0 L 0 0 V. 0 0 S E R V A I 1 0 N S ORGAN NAME KEYWORDS. ;E;E;TT . DISTRIBUTION GROSS FREE-TEXT COMMENTS'% &/ 6 HISTOPATHOLOGIC Fl ------------------------------------------------------------------------------------------------------------ GENERAL COMMENT c C.ICO*Sl-:iL@ iliS6Ei/6ft6AiS'N61tkA@ THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-E04A-RKA-BLE - AT- MI-CROS-COPIC EXAMINATION: BRAIN 1,,COAGULATING GLO (CO). LF. TESTIS ITEO). LIVER ILI), LUNG ILU). PROSTATE IPP). SEMINAL VESICLE IS UPETHRIBR(U -------- -------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA PR HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67S33 SEX: 14ALE STUDY DAY OF DEATH: DOSE 96 GROUP: 2 STUDY WEEK SACRIFICE STATUS: OF DEATH: 14 SCHEDULED. TERMINAL SACRIFIC TERMINAL BODY WEIGHT: NOT EN DATE Of DEATH: 03/25/91 DATE AND TIME OF NECROPSY: (2S/91 9:23 PROSECTON: JACK SERFORT RECORDER: JOE N. KIZZICE POST-FIX WEIGHER: NOT AVAILA0133 E PATHOLOGIST: DR. 70H PALMER WEIOHER: STEVE VAN ADEST ------------------------------A-BS-O-LU-T-E---OR-G-AN---W-EI-G-HT------OR-G-AN---WE-I-G-HT---R-EL-A-71-V-E------O-R-GA-N---TO--G-R-AI-N-----------0--p-a----------- ORGAN NAME (GRAMS) TO BOD-Y--W-EI-G-H-T - 1%) WEIGHT RATIO -------- STA T ------- ---------------------- ------- BRAIN (SRI 2.0130 1.0000 1.1927 EXCLUDE EXCLUDE LIVER ILIJ II.S414 1.4900 ------- .7180 EXCLUDE LUNG (LUI LF. TESTIS -ITEO1 1.6940 -------- .8140 .1038 EXCLUDE EXCLUDE RT. TESTIS TE 1.6749 3.SSIS 1.7074 EXCLUDE -------AC-C-ES-S-/-SE-X---O-R-G---IS-O-)----------------------------------------------------------------------------------------- P A I " 0 L0 0 T 0 8 S E R V A I 10 H S ORGAN NAME KEYWORDS. ;EOVERITY. DISTRIBUTION GROSS FREE-TEXT COM"ENT; */* "ISTOPATHOLOGIC FIN ----------------------------------------------- ------------------------------------------------------------ GENERAL GENERAL COMMENT GIGOCSL:-AL@ INFORMATION (XXI GROSS: iliS6Ei/6lt6AiS*N6RP*4AL . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . . .. .. .. .. .. .I . .. .. . .. . .. .. .. .. .. .. . .. .. .. .. . NECROPSY NOTE: )@TERHIMAL BODY WEIOHT INADVERTENTLY NOT TAKEN PRI COLLECTION AND NECROPSY - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN OR) COAGULATING OLD (CO). LF. TESTIS ITEO). LIVER (LI). LUNG ILU). PROSTATE IPA). SEMINAL VESICLE IS -------U-R-E-TH-R-I--J-U-A-I-------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PA MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7534 SEX: MALE DOSE OROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST POST-REBCIOD DATE OF DEATH: 05/16/91 STUDY DAY OF DEATH: 141 STUDY WEEK OF DEATH: 22 TETMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 05 16/91 8:20 PROSECTOR: DEBBIE PIRKEL RECORDER: JOE M. KIZZIE POST-FIX WEIGHER: NOT AVAILAO(I PAY14OLOGIST: DR. TOM PALMER WEIGHER: JACK SERFORT ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME (GRAMS) ---------------------- -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Ra S TA ------ BRAIN (BRI 2.1879 .3549 % 1.0000 WEIGHT LIVER (LI) 17.1901 2.8139 % 7.9294 WEIGHT LUNG LUI 1.7502 .2865 % .1073 WEIGHT LF. TEIT OITE 1.1921 .3097 x .8721 WEIGHT RY. TESTIS T E ol 1.8614 .3047 % IS98 W[lamt ACCESS/SEX 13 ISO) 3.5869 SI72 % 1.6546 WEIGHT ------------------------------------------------------------------------------------------------------------------- P A 7 H 0 L 0 0 Y' 0 0 S E N V A T 1 0 N S A A A OROAN MA14E KEYWORDS. 6SEV6ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Ft ----------------------------------------------------------------------------------------------------------- GENERAL COMMENT Gi'OCSI:-ALL il@SOE@/6R6AkS'N6RkAi - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN JORI, COAGULATING OLD ICO), LF. TESTIS (TEO). LIVER ILI). LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE URETHR JUR) ------------------------------------------------------------------------------------------------------------ co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATOITIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67535 SEX: MALE DOSE GROUP: 2 DATE OF DEATH: 03/22/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 93 03 22/91 1:10 PROSECTOR: STUDY WEEK RUTH OFSACDREIAFTIHC:E 14STATUS: SC14EDULED TERMINAL SACRIFIC TERMINAL 6ODY WEIGHT: S49. POST-FIX WEIGHER: NOT AVAILAO(E SCHNITZ RECORDER: JOE N. KIZZIEE ------------------------------------------PA-T-H-OL-O-G-IS-T-:---D-R.--T-O-M--P-A-LM-E-R----------------W-EI-G-H-ER-:---J-IL-L--B-E-V-AN--------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) OROAN TO BRAIN 0 aa ---------------------BRAIN (OR) -------- -------- WEIGHT RATIO -------- S TA T ------- LIVER ILI) LUNO ILU) 2.1087 17.0119 1.097S .3139 % 3.0970 % 1.0000 8.0675 WEIGHT WEIGHT LF. TESTIS 1.913S .3090 % .1050 WEIGHT NT. TESTIS ITTEEOIJ ACCESS/SEX 0 (1 SO) 1.9137 .3484 % .3414 % .0074 .0075 WEIGHT WEIGHT -------------------------------------3-.-5-4-2--0------- - - - - - .6448 % - ------ ----- - - - -- - 1.6797 WEIOHT I ORGAN NAME P A 7 N 0 L00 V KEYWORDS. ;EOVERITY. DISTRIBUTION 0 0 S E N V A T 1 0 N S---;-;-;--------------------- ----------------------------------------------------------O-R-O-SS FREE-TEXT COMMENTS / "ISTOPAT"OLOGIC FIND OENERAL COMMENT ------------------------------------------------- GAGOCSI:-ALi iliS6Ei/bltdAiS*N6RitAi . . . . ..... . . . . . . . . . . . . . . . . . . . . . . . . . - -THE- FO-LLOW-THO- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - - BURREATI"NRI BRIIUOICOAGULATING OLD ICUP, LF. TESTIS (TEO). LIVER ILI). LUNG (LU), PROSTATE (PRI, SEMINAL VESICLE (SV --------------------------------------------------------------------------------------------------------------------- to INDIVIDUAL APPENDIX D ANlt4AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?536 SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED. TERMINAL SACRIFIC STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: S?O. DATE Of DEATH: 03/22/91 DATE AND TIME OF NECROPSY: 03 22/OL 1-.35 PROSECTOR: RUTH SCHMITZ RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PAL14ER WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R a ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S IA T ------- BRAIN JON 2.1616 .3790 % 1,0000 WEIGHT LIVER LUNG ILU) 11.4330 I.GSOO 3.2323 % .2909 % I.S278 .7675 WEIGHT WEIGHT LF. TESTIS 'ITEO) 1.8364 .3220 % .8490 WEIGHT RY. TESTIS O@TELISO) 1.1160 .3307 % .8725 WEIGHT ACCESS/SEX 0 3.0024 .5265 % 1.3190 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T " 0 L 0 0 Y 0 8 S E N V A T 10 H S ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION --------------------------------------------------------- GROSS FREE-TEXT COMMENTS "ISTOPATHOLOGIC FIM ------------------------------------------------ GENERAL COMMENT GI(OICSI: -iL@ iliS6E/60tdAiS*N61tkAi . . . . . . * . . * * . .. .. . - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - - - GRAIN,,tiBll,,COAQULATING GLO (CGI. LF. TESTIS (TEO), LIVEN (Lll. LUNG (LUI. PROSTATE IPRI, SEMINAL VESICLE (S -------U-PE-T------u----------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S,A. -------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C$7537 SEX: HATE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC STUDY WEEK Of DEATH: 6 TERMINAL 6ODY WEIGHT: 3S9. DATE OF DEATH:,0F1424/91 STUDY DAY OF DEATH: 36 DATE AND TIME ECROPSY: 01(24/91 9:00 PROSECTOR: KEVIN BILLINGS RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILAS E PATHOLOGIST: DR. TOM PALMER WEIGHER: DEBBIE PINKEL -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIOHT RELATIVE ORGAN TO BRAIN 0Ra ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA T ------- BRAIN on 1.9303 .5301 % 1.0000 WEIGHT LIVER ILII 12.$SIS 3.SISI % 6.6014 WEIOHT LUNG JLUI LF. TE RT@ TESTTI,S E?l ITTE 1.4473 1.6041 1.8330 .4025 % .4461 % .4541 % .7498 .8310 .8460 WEIGHT WEIGHT WEIGHT ACCESS/SEX 0 0 Sol 2.OISS ........5.0.0S..%.............1...04.4.2...............W.E.IG.HT..... --------------------------------------------------- P A T H 0 L 0 0 Y 0 8 S f N V A I 10 N S & * A O-R-OA-N---NA-M-E -------------I--(-E-YW-O-R-DS-.--;-E-AV-E-R-IT-Y-.--DI-S-T-RI-B-U-TI-O-N----7-7------ GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC Ft ------------------------------------------------ KIDNEY(S) (KDI GR6Si:*-iAiGi OEiViSiEit' * * * * * ' * * * ' * *RIiGHAT:' i007i" *CO6tt1EC*YE.D .. .. . . MICRO: -PRESENT -DILATATION.PELVIC THE- FO-LLO-WING- -TISS-UES- WE-RE -UNN-E04A-RKA-13LE- AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON-.- - - - - - BRAIN COAGULATING OLD (CO). LF. TESTIS (TEO). LIVER ILII. LUNG ILU). PROSTATE IPR). SEMINAL VESICLE IS -------U-RE-T-----(-U-R-I--------------------------------------------------------------------------------------------------- C)O INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES At4ERICA. INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CGIS3$ SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE OF DEAT14: 05/16/01 STUDY DAY OF DEATH: 141 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: 692.6 DATE AND TIME OF NECROPSY: OS t$/91 1:37 PROSECTON: GAIL M. NOVAK RECORDER: JOE H. KIZZIEE POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOOTST: DR. TOM PALWA WEIGHER: JACK SERFORT ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO GRAIN 0 P0 A ORGAN NAME ---------------------- (GRAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S IA T -------- BRAIN ILBINJ 2 2904 .3307 % 1.0000 WEIGHT T LIVER 20.1006 2.9137 % 8.8109 WEIGHT TI LUNG (LUI l@9530 .2820 % .8527 WEIGHT I LF. TESTIS OITE?ISOI 1.850S .2672 % .8070 WEIOHT OT. TESTIS TE 1.7197 .2483 % .7508 WEIGHT T ACCESS/SEX 0 3.8643 sslo % 1.0172 WEIGHT T ------------------------------------------------------7------------------------------------------------------- P A T " 0 L 0 0 Y 0 8 S t R V A T 10 N S 0 A & O-R-GA-N---MA-W--------------K-E-Y-WO-R-D-S.---'S-E-V*E-R-'I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R-O--S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-IC----FI-N-D------- GENERAL LIVER COMMENT GI(OICSI-:ALE iliS6Ei/6lt6AhS*N61ti4AL .. . .. .. (LIJ . . . . . . . . . . . .. .. .. . .. .. .. . .. .. .. . ... . .. .. . ... .. .. MICRO: -SLIGHT, FOCAL -INFILTRATES.MONONUCLEAR CELL THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN (SRI. COAGULATING OLD (COI, LF. TESTIS (TEOI. LUNG (LU), PROSTATE (PR). SEMINAL VESICLE ISV), URETHRA -------- ----------------------------------------------------------------------------------------------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. p MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL HU14BER: CS?539 SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM S TERMINAL 6ODY WEIGHT: SIO DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: S7 STUDY WEEK OF DEATH: 9 PROSECTON: KEVIN BILLINGS RECORDER: JILL BEVAN DATE AND TIME OF NECROPSY: 02 14/91 7:58 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER: VAL QUINN ---------------------------------------- ------------------------------------------------ - ---- --- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Aa ORGAN NAME (GRAMS) ------ TO BOD-Y--W-E-I-GH-T- 1%1 WEIGHT RATIO ------ STA -----------BRAIN ---- 0 2.03SO .3986 % 1.0000 I;i-t-C-H-T LIVE 10LIJ 17.49$S 3.4204 % .?194 WEIGHT WEIGHT LUNG ILU) LF. TESTIS '(TEO) 1.8089 1.7637 .3146 % .34S3 % .8664 WEIGHT WEIGHT NT. TESTIS ITEII 1.7937 .3512 % SS46 % .1112 1.6423 WEIGHT ------A-C-CE-S-S-/S-E-X---O-RO---(-S-O-)--------------3.-3-4-31-------------I------------------------------------------------- ftA ft P A T " 0 L 0 0 y 0 0 S E N V A T 1 0 N S a 0 0 O-R-GA-N---NA-M-E--------------KE-Y-W-OR-D-S-. --S-E-VE-R-I-TY-,---DI-S-T-RI-B-U-TI-O-N------------ GROSS FREE-TEKT COMMENTS / "ISTOPATHOLOGIC ri ----------------------------------------------- GENERAL COMMENT c GI(olst:-AL@ iliS6Ei/6RdAkS'N6RAA@ LIVER ILI) :IiFiLiNATiS*N6N6N6CiEAlt'CiL@ - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - BRAIN IBRI. COAGULATING OLD (CO). LF. TESTIS 17EO). LOHO ILU). PROSTATE IPP). SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------------------ co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. pp MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- AN104AL NUMBER: CS?540 SEX: 14ALE DOSE ONOUP: 2 SACRIFICE STATUS: SCHED LED SECOND INTERIM SA DATE OF DEATH: 02/12/91 STUDY DAY OF DEATH: SS $TTODY WEEK Of DEATH: I TERMIUmAL iODY WEIGHT: 552. DATE AND TIME OF NECROPSY: 02(EI2/91 9:42 POST-FIX WEIGHER: NOT AVAILAO PROSECTON: NANCY DIEDRICH PATHOLOGIST: DR. TOM PAL14EN RECORDER: DENNIS HOFFHAP -------------------------------------------------------------------------W-EI-O-H-ER-:---P-A-TH-O-LO-G-Y---DE-P-A-R --------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 pa OROAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- STA T ------- BRAT N IBN11 LIVER L 2.1032 24.8735 .3$OS % 4.4042 % 1.0000 11.7314 WEIOHT WEIGHT LUNG ILUI 1.1603 .3366 % .1145 WEIGHT LF. TESTIS OITEEOIISOI 1.6493 .2984 % .7842 WEIGHT AT. TESTIS T 1.6410 .2970 % .780S WEIGHT ------A-C-CE-S-S-/S-E-X----0--------------------3.-0-2-3-S -------------------.5-4-7-0-%---------------1.-4-3-70-------------WE-I-G-HT------------------ OkA * P A T " 0 L 0 0 Y' 0 B S E N V A T 1 0 X S 6 a a ORO-AN---N-AM-E-------------K--E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.--D-I-S-TR-I-B-UT-I-O-N----------G-RO-S-S FREE-TEXT COMMENTS / HISTOPAT"OLOGIC Ft 14ANDIB/CERV LN ------------------------------------------------ SN"O"IS:.-iAiC;i. . . . . . . . . . . . . . ..... .L.E.F.T:.U.P-TO-15.X 10.X.S.M.M (-FO.R.CO'M-PAI'tt'iSlOdihi MICRO: -MODERATE, MULTI-FOCAL x 2 MM) CAP 61 HYPERPLALIA. LYMPIFORETICULAR CELL THE- FO-LLC-WI"O- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT MICROSCOPIC EXAMINATION: BRAINHRIBRI, COAGULATING OLD (CO). LF. TESTIS (TEO). LIVER (LI). LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE (S URET (URI -------- --------------------------------------------------------------------------------------------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67541 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF DEATHI 03/22/91 STUDY DAY Of DEATH: 03 STUDY WEEK OF DEATH. 14 TER141MAL 600Y WEIGHT: 562. DATE AND TIME OF NECROPSY: 03/22/91 0:42 PROSECTOR: KEVIN BILLINGS RECORDER: JOE M. KIZZIEE PO-S-T--F-I-X--W-E-IG-H-E-R:---N-OT--A-V-A-IL-A-B-LE-----------------P-AT-H-O-LO-G-I-S'-T----D-R-. --TO-M--P-A-LM-E-R----------------W-E-IG-H-E-R:---J-I-LL---BE-V-A-N -------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S--T--A--T- BRAIN JLONli LIVER 2.1370 16.4804 .3102 % 2.9319 % 1.0000 7.7119 WEIGHT WEIGHT LUNG ELLUI I.IS20 .3205 % .8066 WEIGHT LF. T TI 1.5904 .2829 % .7442 WEIGHT PT. TESTIS TE l.se6o .2710 X .7328 WEIGHT ACCESS/SEX 01TOcollsol 3.7492 .8741 % 1.7731 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T " 0 L 0 0 Y, 0 0 S E A V A T 1 0 N S a a a ORGAN -NA-M-E -------------K-E-Y-WI-D-I-TD-S-.-;-E-&V-E-&-RI-T-Y.--D-IS-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-O-PA--T-"--O-L--OG-I-C---F-l--- GENERAL COMMENT GI(OICSI-:iL@ itiS6Ei/6RdAiS'N6RAA@ . .. .. .. - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN,,IBII,,COAGULATIMO OLD fco 1. LF. TESTIS (TEO). LIVER (LIJ. LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE IS -------U-RE-T------u------------------------------------------------------------------------------------------------ co L" INDIVIDUAL APPENDIX 0 AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7542 DATE OF DEATH: 02413/91 SEX: MALE DOSE GROUP: 2 STUDY DAY OF DEATH: SO SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE AND TIME OF CROPSY: 02(13/91 8:13 STUDY WEEK OF DEATH: I PROSECTON: RUTH SCHNITZ TERMINAL 600Y WEIGHT: 432. P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-OT--A-V-A-IL-A-D--E----------------P-AT-H-OL-O-O-IS-T-:---D-R.---TO-M--P-A-LM-E-R----------------RW-EE-CIOG-RH-DE-ERR:-:--J-IKL-ELV-I-NB-E-VB-AIN-LL-I-N-GS----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORANS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (1) ORGAN TO BRAIN 0Ra ---- ------ ----- WEIGHT RATIO S TA T BRAIN (OR) LIVER ILI) 1.0704 14.974S .4552 % -----WEIGHT LUNG ILU) LF. TESTIS 1.5792 3.4SOI % .3048 % 7.5997 .8ols WEIGHT WEIGHT RT. TESTIS TEI0I 1:6601 1.7024 .383S % .842S WEIGHT ACCESS/SEX 0 a Sol 2.2701 .3033 1 .5244 % .1640 WEIGHT -------------------------------------------------------------------------------I.-I-S-2-1------------WE-I-G-HT------------------ ORGAN NAME P A T H 0 L 0 0 Y- 0 8 S E A V A T 1 0 N S .4a a KEYWORDS, S'EV6ER'ITY. DISTRIBUTION G-E-N-E-R-A-L-C-O-M-M-E-N-T----c-----------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-OP-A-T-HO-L-O-OI-C----FI-N-[------- GA'oosl:-ALi fliS6Ei/6lt6AiS'M61ti4AE. . . . THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON- - - - - - - - - - - - - - - BUPREATIHNRI OR()OA)COAGULATING OLD (CO). LF. TESTIS (TEO). LIVER (LI), LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE ISV --------------------------------------------------------------------------------------------------------------------- OD APPENDIX D INDIVIDUAL AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ADANIMAL NUMBER: C67543 TE OF DEATHE: orOS4EIS/91 SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE AND TIM CROPSY: STUDY DAY OF DEATH: 141 STUDY WEEK OF DEATH: 22 8:22 PROSECTON: GAIL TERMINAL 6ODY WEIGNT: IZ146.2 POST-FIX WEIGHER: NOT AVAILgSB(Ele/ol M. NOVAK -----------------------------------------P-A-T-HO-L-O-GIST: DR. TOM PALMER RECORDER: JOE m, x IEE WEIGHER: JACK SINFORT ------------------------------------------------------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R 0A ---------------------- -------- TO BODY WEIGHT -------- WEIGNT RATIO -------- S TA T BRAIN LIVER 2.2020 16.OS93 .4033 % 1.0000 -------WEIGHT T LUNG ILU) 1.7220 3.IOSO % 7.0990 WEIGHT T LF. TE tis OITEOJISO) RT. TESTIS TE 1.4962 .3154 % .2739 % .7220 SY92 WEIGHT T ACCESS/SEX 0 I.SO67 2.3164 .2759 % 6840 WEIGHT T WEIGHT T -------------------------------------------------------------4.3-.6-.9-.-%.-.-.-.-..........1'.0.1.33 WEIGHT TJ O-R-GA-N--N-A-M-E -------------K--E-Y-WO-R-D-S.---;-E-VE-R*--IT-Y-.P--DAI-S-TT-RH-IB-0U-T-ILO-N0--0--V----0--0-G-RS-OS-ES R VA T 1 FREE-TEXT 0 N S -;-;-;--------------------COMMENTS / HISTOPAT"OLOOIC FINDI GENERAL COMMENT - ------------------------------------------- - - - - -GI'-O'C-sl-:A-LE iliS6Ei/6R6AiS'Ni)RkAE - -- . .. . .. ... .. . .. .. .. .. .. . THE FOLLOWING TISSUES WERE UNREMARKABLE AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - - BURREATI"NRiiBRJIUA)COAGULATING OLD (COI. LF. TESTIS (TEO). LIVER (LI). LUNO (LUI. PROSTATE (PRI. SEMINAL VESICLE ISVP ---------------------------------------------------------------------------------------------------------------------- OD 4 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUM13EN: CS7S44 DATE OF DEATH: 01/25/91 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACR STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 DATE AND TIME OF NECROPSY: 01 2S/91 8:44 PROSECTON: NANCY DIEDRICH TERMINAL 6ODY WEIGHT: 317.7 POST-FIX WEIGHER: NOT AVAILAB(E PATHOLOGIST: 01. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-EI-G-H-ER-:---P-AT-H-O-LO-G-Y---DE-P-A-R ----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) OftOAN WEIGHT RELATIVE ORGAN TO BRAIN 0 N0 A ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- STA T -------- BRAIN (ON) LIVER (LI) 2.0234 12.9862 .5219 % 1.0000 WEIGHT T LUNG (LUI 1.4677 3.3405 % .3780 X 6.4110 .7254 WEIGHT I WEIGHT LF. TESTIS TE AT. TESTIS TE 1 I.S412 1.6271 .39?S % .4197 .7617 T WEIGHT T ACCESS/SEX 01 0 olsol 2.S267 X Isil % .8041 1.2417 WEIGHT T -----------------------------------------------------------------------------------------------W-EI-G-HT---I---------------- ORGAN NAME P A T H 0 L 0 0 Y' 0 6 S t A V A T 1 0 N S kt a A KEYWORDS. S*EV0ERAITY. DISTRIBUTION --------------------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-OP-A-T-"O-L-O-QI-C---F-I-N-D------- GENERAL COMMENT c LIVER (LI) al(ols-tA:Li fliS6Ei/61ldAiS'N.6P.tk.AE. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M16NO: -MINIMAL, MULTI-FOCAL -INFILTRATES.MOMONUCLEAR CELL - -THE- FO-LLOW-ING- T-ISS-UES -WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - -------B-R-A-I-N --I-ON-)-.--CO-A-G-U-L-AT-I-N-G--G-L-D--(C-G-)-.--L-F.--T-E-S-TI-S---(T-E-O-)-.-L-U-N-G--(-L-U)-,--P-R-O-ST-A-T-E--(-P-RI-.--S-E-M-IN-A-L--V-E-SI-C-L-E--I-S-V-I,--U-R-E-T-HR-A------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C07545 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA( DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 DATE AND TIME OF NECROPSY: 02(14/91 7:37 PROSECTOR: DENNIS HOFFMAN TERMINAL AODY WEIGHT: 436. RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: DR. TOM PALMER WEIGHER: VAL QUINN -------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIOHT #GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN WEIO"T RATIO 0 Ra 0 S IA I ---------------------- -------- -------- -------- -------- BRAIN (ON) LIVER (LI) 2.0517 .4099 % 1,0000 I WEIGHT I LUNG (LU) le.oOS3 1.5610 3.6659 % .35?S % 1.8010 .7608 WEIGHT I LF. TESTIIS OITEO JISOI 1.7831 .4014 % .8691 WEIGHT I WEIGHT NT. TEST S TE ACCESS/SEX 0 1.8184 3.1652 .416S X .7250 % .8863 1.5427 WEIGHT TI WEIGHT --------------------------------------------------------------------------------------------------------------------- PA I"0 L0 0 Y 0 0 SE RV AI 10N S A aa ORGAN NAME ---------------------- KEYWORDS. ;EVAER*ITY. DISTRIBUTION ----------------------------------t------ GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOOIC FIND ------------------------------------------------- GENERAL COMMENT c 01(ol.st:.-A.L@. i.li.S6.Ei/.6R.6A.iS.*N6.Ri.lA.E . LUNG (LU) . . . GROSS: NiCiOOSi liOiE: )Tili*4il#6 6Oi4MiNi: 'OiE'SiCil6N'Oi El@Ei AtiSiNd iR AT TIME OF TRIMING - -THE- FO-LLOW-ING- T-ISS-UES - WERE - --UNREMARKABLE - ---- --- -AT MICROSCOPIC EXAMINATION-. - - - - - - - - - - - - - - - BRAIN SRI COAGULATING URETHRI (Uil OLD (CG). LF. TESTIS (TEO), LIVER (LI). LUNG (LU). PROSTATE IPP). SEMINAL VESICLE (SV --------------------------------------------------------------------------------------------------------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANlP4AL NUMBER: COYS4$ DATE OF DEATH: 02413491 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I DATE AND TIME OF EC OPSY:,02(13/01 9:03 PROSECTOR:,,RUTH SCHNITZ TERMINAL 6ODY WEIGHT: 476. POSI-FIX WEIGHER: NOT AVAT AIB E PAT"OLOG S : DR. TOM PALMER RECORDER: KEVIN BILLINGS --------------------------------------------------------------------------------W-E-IG-H-E-R:---J-I-LL--B-E-V-AN--------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0Ra ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA 7 ------- BRAIN 1L8;l LIVER 2.IIS7 .4436 % 1.0000 WEIGHT LUNG ILU) 16.1950 1.538S 3.3050 1 7.BS47 WEIGHT LF. TESTIS TEO1 1.4111 .3226 X .3541 x .7272 WEIO"T RT. TESTIS TE ACCESS/SE x 010 ISO) 1.6887 .3541 % .1912 .7912 WEIGHT WEIGHT -------------------------------------2.-7-0-0-4-------------------.5-0-7-0-%---------------1.-2-1-01-------------WE-I-G-HT------------------ ORGAN NAME P A T N 0 L 0 0 V 0 B S E N V A T 1 0 N S a ik a KEYWORDS. ;E*VERITY. DISTRIBUTION G-E-NE-R-AL---C-O-MM-E-NT-----------------------------------------------O--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-OP-A-T-HO-L-O-GI-C---F-I-N------- LIVER (LI) GI(OICSI-:AL@ iliS6Ei/bit6AiS*N6RM*AL' .. . . . . . . . . . . . . . . . . . . . . . . . . . . -INFILTRAT.ES..MO.NO.NUC.LE.AR CELL THE- FO-LLOW-THO- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - BRAIN JOR). COAGULATING OLD ICG). LF. TESTIS (TEO), LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C67S47 DATE OF DEATH: 02114191 SEX: MALE DOSE GROUP: 2 STUDY DAY OF DEATH: 57 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE AND TIME OF NECROPSY; 22(14/91 8:59 STUDY WEEK Of DEATH: 9 PROSECTOR: KEVIN BILLINGS TER14INAL 6ODY WEIGHT: 484. POST-FIX WEIGHER: NOT AVAIL 0 E PATHOLOGIST: 00. TOM PALMER RECORDER: JILL BEVAN WEIGHER: VAL QUINN ------------------------------------------------------------------------------------------------------------------- ORGAN NAME ---------------------- BRAIN (OR) LIVER ILIJ LUNG LF. TE LIUII fTE01 RT. TESTTS (TELI ACCESS/SEX ORG ISOI ABSOLUTE 00(IAN WEIGHT (GRAMS) -------- 2.1074 IS.3574 1.8518 I.Slis 1.6548 2.5313 ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 N0 S IA T ------- .4s34 1.0000 WEIGHT 3.3041 1 7.2874 WEIGHT .3SS4 X .7838 WEIGHT I .3397 % .7492 WEIGHT 7 .3560 % .7852 WEIGHT T S446 % 1.2011 WEIGHT T ------------------------------------------------------- ORGAN NAME PA TH 0 L00 Y 0 0 SE NV A T10N S KEYWORDS. ;E'VERITY. DISTRIBUTION G-E-NE-R-A-L --C-O-MM-E-N-T ---(-O-C-----------------------------------------G--R--OS--S---FR-E-E--T-E-XT---C-O-MM-E-NT-S-------HI-S-T-OP-A-T-HO-L-O-GI-C----FI-N-D------- GROSI: -AL@ iliS6lEi/bltdAiS*N6lti4Ai. . . . .. .. .. .. .. .. . .. .. .. ... THE- f0-LLO-WIN-G T-ISS-UES- WE-RE -UNR-EMARKA OLE AT MICROSCOPIC EXAMINATION: - --- -- ---- -- BUNREATIHNRI BRJIUA) COAGULATING GLD (CGI. LF. TESTIS (TEO). LIVER (LIJ, LUNG ILUI. PROSTATE IPRI. SEMINAL VESICLE lsv -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CSIS4$ DATE OF DEATH:OOSF417/91 SEX: MALE DOSE GROUP: 2 STUDY DAY OF DEATH: 149 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE AND TIME ECROPSY: 05 17/91 7:09 STUDY WEEK OF DEATH: 22 PROSECTOR: DEBBIE PINKEL TERMINAL 6ODY WEIGHT: Sao. POST-FIX WEIGHER: NOT AVAILAO(C PATHOLOGIST: ON. TOM PAL14EN ItECORDElt: JOE N. KIZZIEE -------------------------------------------------------------------------W-E-I-(I-H-E-R:--J-A-C-K--S-ER-F-O-RT------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 Ra ------------------- -------- -------- WEIGHT RATIO -------- STA T ------- BRAIN JOR) LIVER ILI) 2.2262 17.9184 .3970 % 3.2082 % 1.0000 1.0103 WEIGHT WEIGHT LUNG ILU) LF. TESTIS T NT. TESTIS OITE'I*ISO, I.SIS4 1.7319 .2703 % .3100 X .6807 .7107 WEIGHT WEIOHT ACCESS/SEX 0 1.7066 3.5678 .3044 % .7666 WEIGHT T ------------------------------------------------------1-----------.--6-3-6-3--%--------------1-.-6-02-8-------------WE-I-GH-T---T------- ORGAN NAME P A T " 0 L 0 0 Y 0 8 S E N V A T 10 N S * h * KEYWORDS. ;E&VERNITY. DISTRIBUTION GENERAL- -C-O-MM-E-NT------c----------------------------------------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-O-IC----FI-N-D------- LUNG (LU) Gloost: -AL@ iliS6Ei/61tdAiS*N6RI@AE dit6si:' *NE,CN*O,PSY* 1,#O*TE:* C*OM*ME,N,T:,M,IS,SI,NG,FR*O,*MWE*T T*I'SS'UE'A'T'TIME THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON-- - - - - - BUNREATIHNR JOR()U.RICOAGULATING OLD (CG), LF. TESTIS (TEO). LIVER (LI). LUNO (LUI, PROSTATE (PRI. SEMINAL VESICLE ISV --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL AIPPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67549 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED. TERMINAL SACRIFIC DATE OF DEATH: 03/25/01 STUDY DAY OF DEATH: 90 DATE AND TIME OF NECROPSY: 03/2S/91 9:12 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: Soo. POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: JACK SERFORT PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE --------------------------------------------------------------------------------W-EI-G-HE-R-:---S-TE-V-E--V-AN--A-D-E-ST----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN S0INAaI ---------------------BRAIN JLOIRJ -------2.261093 -------.3701 % WEI-GH-T---R-AT-I-O 1.0000 ------WEIGHT LIVER LUNG ILU) 23.4 2 1.7373 3.0424 % .2850 % 10.3611 .7686 WEIGHT WEIGHT LF. TESTIS RT. TESTIS T 011 229S 11-:22 .2991 % BooS WEIGHT ACCIESS/SEX 0 0 Sol 3.9714 .2990 % .6510 % .1063 I.YS70 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 0 Y' 0 0 S E R V A T 1 0 N S 0 & .0 KEYWORDS. ;E*VERITY. DISTRIBUTION G-E-NE-R-AL---C-O-MM-E-NT-----------------------------------------------G--R-O--S-S---F-RE-E---TE-X-T--C-O-M-KN-T-S----/---HI-S-T-O-PA-T-H-OL-O-G-I-C ---FI-N-I------ LIVER (LI) GI(OICSI: -ALE iliS6Ei/i)R6AiS*N6RkA@ . . . . .- MICRO: klii*MAL*.,F,OCA,L -'IN*FI,L*tRA*TE,S.*M,ON,ONU,CL,EA,R,CE,L*L THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - -------B-R-A-IN---IB-R-I-.--CO-A-G-U-L-AT-I-N-G--O-L-D --(C-o-l-.--LF-.--T-E-S-TI-S--I-T-E-O-).--L-U-N-G--(-L-U)-.--P-R-OS-T-A-T-E--(-P-RI-.--S-E-M-IN-A-L--V-E-SI-C-L-E--I-S-V-I.--U-R-ET-H-RA------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67550 DATE OF DEATH: 01/2S/91 SEX: t4ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED STUDY DAY Of DEATH: 37 STUDY WEEK OF DEATH: 0 FIRST INTERIM SAC DATE AND TIME OF NECROPSY: 01(25/01 1:10 PROSECTON: DENNIS HOFFMAN TERMINAL 6ODY WEIGHT: 387. RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAS E PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY DEPAR -------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT IGRANS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN WEIGHT RATIO 0 0a ---------------------- -------- -------- -------- S TA T ------- BRAIN 1L8IJ 1.9897 S129 % 1.0000 WEIGHT LIVER 14.1445 3.6464 % 7.1019 WEIGHT LUNG LF. TE Ti TE 1.5451 1.5024 .3983 % .3173 % .776S .7ssl WEIGHT NT. TESTIS TEI 1.4836 .3825 % .7456 WEIGHT WEIGHT ACCESS/SEX 0 0 SO) 2.526S .$SI3 % 1.2698 WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 0 Y@ 0 0 S E N V A T 1 0 N S A a lb KEYWORDS, ;E*VERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPAT"OLOGIC FIN ------------------------------------------------------------------------------------------------------------ GENERAL COMMENT (1400CSI-:ALi iliS6Ei/6lt6AiS*N6ltkA@ - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN JOR ),,COAGULATING OLD (CO), LF. TESTIS (TEO). LIVER (LI). LUNG (LU), PROSTATE IPO), SEMINAL VESICLE IS URETHRA IU -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMALNUMBER:C67551 DATE OF DEATH: 03/25/91 SIX: MALE DOSE GROUP: 2 STUDY DAY OF DEATH: 96 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE AND TIME OF NECROPSY: 03/25/91 10:08 STUDY WEEK OF DEATH: 14 PROSECTON: JULIE A. LARSEN TERMINAL BODY WEIGHT: S$3.4 RECORDER: JOE N. KIZZIEE P-O-S-T--F-IX---W-EI-G-HE-R-:---NO-T--A-VA-I-L-A1-3-L-E ----------------PA-T-H-OL-O-G-IS-T-:---0-4.--T-0-1-4-P-AL-M-E-R----------------W-EI-G-H-ER-:---ST-E-V-E--V-AN--A-D-E-ST-I----------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R0 A ORGAN NAME ---------------------- JORAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA I -------- BRAIN ILO1N1 LIVER 2.3262 17.3SS2 .3917 % 1.0000 WEIGHT T LUNG (LU) 1 5754 2.9800 % .2700 % 7.4736 .6772 WEIGHT T WEIGHT I LF. TESTIS TEOI AT. TESTIS 0 TE 1.7933 1.82S7 .30?4 % .3129 % .7709 .7841 WEIGHT T ACCESS/SE X 10 ISO) 4.3740 .7497 % 1.8003 WEIGHT 7 WEIGHT T --------------------------------------------------------------------------------------------------------------------- P A I H 0 L 0 0 Y@ 0 0 S E R V A T 1 0 N S a a a O-R-GA-N---NA-M-E-------------K--E-Y-WO-R-D-S,---;-E-6V-E-4R-IT-Y-.--DI-S-T-RI-B-U-TI-O-N----------G-RO-S-S RREE-TEXT COMMENTS / HISTOPAT"OLOGIC FIND ------------------------------------------------GENERAL COMMENT (OC GROSI: -AL@ iliS'UE@/6R6AkS'Piblti4A@ .. .. .. THE- FO-LLO-WING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - - BURREATIHNR JORf)u,n)COAGULATING OLD (CGI, LF. TESTIS (TEO). LIVER (LI), LUNG ILUP, PROSTATE (PA). SEMINAL VESICLE (SV INDIVIDUAL APPENDIX D ANIMAL PATIIOLDOY DATA HAZLETON LABORATORIES AMEftICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67552 DATE OF DEATH: 03/26/91 SEX: 14ALE D9OSE (INDUP: 2 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC STUDY DAY OF DEATH; 1 DATE AND TIME OF NECROPSY; 03 26/91 9:08 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: 610, POST-FIX WEIOHER: NOT AVAILAO(E PROSECTON. STEVE VAN ADESTINE PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN ---------------------------------------------------------------------------------------W-EI-G-H-E-R:----JO-E--N-.--K-I-ZZ-I-E-E--------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORANS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 0a ---------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA T BRAIN (SRI LIVER ILI) 2.0215 23.1634 .3313 x 1.0000 ------WEIGHT LUNO ILU) LF. TESTIS OITIEOJISOI 1.1710 2.0816 3.9108 % .1919 % 11.8041 SI93 WEIGHT WEIGHT AT, TEST S TE 2.5160 .3411 % 1.0297 WEIGHT ACCESS/SEX 0 3.$IOS .4238 X 624S % 1.2792 WEIGHT T ------------------------------------------------------------------------------------------1-.-1-1-50--------------W-EI-G-H-T---T--- ORGAN NAME ------- ------GENERAL COMMENT KEYWORDS. P A T H 0 L 0 0 Y' EVERITY. DISTRIBUTION 0 0 S E P V A T 1 0 Pf S a 0 e GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND ------------------------------------------------- LIVER (LI) 01'OCSI:-AL@ iliSuEi/6R6AiS'N61tkAi .. .. .. . .. .. .. . .. ... .. .. .... .. .. . .. .. . .. ... .. . .. .. .. . .. .. .. .. . ... . .. .. . MICRO: -SLIGHT. FOCAL -NECROSIS.COAGULATIVE THE FOLLOWING TISSUES WERE -UNREMARKABLE BRAIN 3*). COAGULATING AT MICROSCOPIC EXAMINATION: --- -- ---- --- --- ------------- ---- OLD -------------- ICOI, ----- LF. --- TESTIS ------ ITEO). ------ LUNG ---- (LU). ----- PROSTATE -------- I-P-P-)-.- S-E-MI-N-A-L-- V-E-S-IC-L-E-- I-S-V-I-.- U-R-E-TH-R-A -- - cm INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67553 DATE OF DEATH: 03/25/91 SEX: 14ALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED TERNI STUDY DAY OF DEATH: 96 DATE AND TIME OF NECROPSY: 03/25/91 8:30 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEINGAHLT:SACRISFOTO POST-FIX WEIGHER: NOT AVAILABLE PPAROSECTOR: JULIE A. LARSEN RECORDER: JOE N. KIZZIE --------------------------------------------T-HO-L-O-GI-S-T-: --D-R-.--T-01-4-P-A-L-ME-R----------------W-E-IG-H-E-R:---S-T-EV-E---VA-N--A-DE-S---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S--T--A-- BRAIN IBR) LIVER (LI) 2.1217 17.9913 .3715 % l@0000 WEIGHT LUNG ILUI LF. T TI 1.7719 3.2093 % .3101 % 1.4797 .13SI WEIGHT WEIGHT RT. TESTIS ITTE?I 1.7765 1.7760 .3169 % .1373 WEIGHT ACCESS/SEX 0 G SO) 3.5994 .3168 % .6421 % .8371 1.69@S WEIGHT -----------------------------------------------------------------------------------------------W-E-IG-H-T ----------------- ORGAN NAME P A T N 0 L 0 0 Y. 0 8 S E R V A T 1 0 N S KEYWORDS, S*EVaER&ITY. DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENT; */* HISTOPAT"OLOGIC Fl GENERAL COMMENT ------------------------------------------------ LIVER (LI) (il(o.lc.s-lA.:Li. i.li.S6.Ei./6.lt.6A.iS.*N.61t.kA.@ . . . . . . . . . . . . .. .. .. .. M16RO: -MINIMAL. FOCAL -INFILiRATis,,M*ONO,NU,C,LEA,R,CE'LL, THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - -------B-R-A-IN---J-OR-)-,--CO-A-G-U-L-AT-I-N-G--G-L-D--(-CG-I-,--L-F.--T-E-S-T-IS---(T-E-O-)-.--LU-N-G--(-L-UI-,--P-R-O-ST-A-T-E--I-P-R-I.--S-E-M-IN-A-L--V-E-SI-C-L-E--I-S-V-I-. -U-R-E-TH-R-A----------------- INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C07554 DATE OF DEATH: 02/13/01 SEX.- MALE DOSE GROUP: 2 SACRIFICE STATUS: ICHEDULED STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I E SECOND INTERIM SA DATE AND TIME OF NECROPSY: 02(13/91 7:45 PROSECTOlt: DENNIS ROFFMAN tt4lNAL IOOY WEIGHT: 496. RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILAO E PATHOLOOIST: DR. TOM PALMER WEIGHER: JILL BEVAN ----------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN WEIGHT RATIO 0 Ra i ---------------------- -------- -------- -------- S TA T -------- BRAIN LIVER 2.1668 19.4011 .4365 x 3.9090 N 1.0000 WEIGHT T LUNG ILU) LF. TESTIS 1.6152 .3254 % i.gs7o .7454 WEIGHT T WEIGHT T RT. TESTIS ITTEE011 1.7070 770 .3439 X .3378 % .7171 .7740 WEIGHT T ------A-C-CE-S-S-/S-E-X---0--0---S-O-)--------------3'-..S-eo-o-l-------------------.7-2-33 % i.es7i WEIGHT t WEIGHT T r ------------------------------------------------------ ORGAN NAME dk A 01 P A T H 0 L 0 a Y 0 0 S E R V A T 1 0 N S KEYWORDS. SEVERITY. DISTRIBUTION A& A ----------------------------------------------------------G--R--O-S-S---F-RE-E---TE-X-T--C-O-M-14-E-N-TS---/---H-IS-T-O-PA-T-1-4O-L-O-OI-C---F-IN-D------- GENERAL COMMENT c .. .. .. .. . .. .. . .. .. .. .. .. .. . ... .. 01(olst:-AL@ iliS6Ei/6R6AiS'N6RkA@ THE- FO-LLO-WING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE AT MICROSCOPIC EXAMINATION-. UBRREATIHNRA ORJ)UA) COAGULATING OLD (CO), LF. TESTIS (TEO). LIVER ILIJ. LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE (SV -------- --------------------------------------------------------------------------------------------------------- OD INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. KADISON. WISCONSIN U S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67555 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE Of DEATH: 01423/91 STUDY DAY OF DEATH: 3S DATE AND TIME OF ECROPSY: 01(23/91 1:08 PROSECTON: STUDY WEEK OF DEATH: NANCY DIEDRICH 5 TERMINAL 6ODY WEIGHT: 422. POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN WEIGHER: GAIL M. NOVAK -------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (ORAMS) OftOAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN WEIGHT RATIO S0 INA0 I ---------------------- -------- -------- -------- ------- BRAIN ( 2.0072 .4755 % 1.0000 WEIGHT LIVER (OLRil 15.7892 3.7406 % 7.1663 WEIGHT LUNG ILUI 1.5776 .3731 % .7060 WEIGHT LF. TESTIS TEO 1.4911 .3S4$ % .7464 WEIO"T RT. TESTIS TEI 1.4914 .3533 % .7430 WEIGHT ------A-C-CE-S-S-/S-E-X---0-10---I-S-O-)--------------2.-7-6-1-0-------------------G-S-41---% --------------1-.3-7-5-5------------W-E-IG-H-T ----------------- ORGAN NAME P A T " 0 L 0 0 Y- 0 8 S E R V A T 1 0 N S e . * KEYWORDS, *SEVAER*ITY. DISTRI13UTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOOIC FIM ------------------------------------------------------------------------------------------------------------- KIDNEYIS) IKDI I . . . . . . . . . . . . . . . . . . . ..... . . . I . . . . . . . . . . . . . . . . . . . . . GROSS: -LARGE PELVIS(ESP -RIGHT: BOTH KIDNEYS COLLECTED MICRO: -PRESENT -DILATATION.PELVIC LIVER ILII . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -SLIGHT, FOCAL -INFILTRATES,MONONUCLEAR CELL THE- FO-LLOW-INO- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN JOR). COAGULATING OLD (CGI. LF. TESTIS ITEO). LUNG (LUI. PROSTATE FPRI. SEMINAL VESICLE (SVI, URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PAT"OLDOY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U-S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COISSO DATE OF DEATH: OF03426/91 SEX: MALE DOSE GROUP: 2 STUDY DAY OF DEATH: 97 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE AND TIME ECROPSY: 03 26/01 8:35 STUDY WEEK OF DEATH: 14 PROSECTOR: STEVE VAN ADESTINE TERMINAL 6ODY WEIGHT: 532. POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN WEIGHER: JOE M. KIZZIEE ------------------------------------------------------------------------------------------------------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Na ---------------------BRAIN ILO1N1 -------2.2605 TO BODY WEIGHT -------.4247 X WEIGHT RATIO -------1.0000 STA T ------WEIGHT LIVER LUNG ILU) 10.9106 1.4190 3.1700 % 7.4830 WEIGHT LF. TESTIS NT. TESTIS ITTEEIOI 1.5240 .2606 % .2463 X .6277 .0742 WEIO"T WEIGHT ACCESS/SEX 000 (SO) 1.5304 3.7276 .2875 % .7003 % .6770 1.6490 WEIGHT WEIGHT --------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A I " 0 L 0 0 Y- 0 8 S E A V A T 1 0 N S 0 6 A KEYWORDS. ;E;ENOITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---"I-S-T-O-PA-T-"-OL-O-G-I-C ---FI-N-D------- GENERAL COMMENT GA(OICSI-:ALi iliS6[i/61ldAiS*N61tl@Ai - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON-- - - - - - - - - - - - - - - BURREATI"NRL BR(IUA)COAGULATING OLD (CO), LF. TESTIS (TEO). LIVER (Lll. LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SV -------- ---------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C67SS7 SEX: 14ALE DOSE ONOUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM S DAIE Of DEATH:OF2/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 DATE AND TIME NECROPSY: 22(14/91 7:29 PROSECTOR: DENNIS HOFFMAN TERMINAL 6ODY WEIGHT: SOS RECORDER: JILL BEVAN P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-OT--A-V-A-IL--8--E----------------P-AT-H-O-LO-G-I-ST-:---D-R-.--T-OM--P-A-LM-E-R----------------W-E-I-GH-E-R-: --V-A-L--QU-I-N-N-------------------- ABSOLUTE ORGAN WEIGHT OROAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ORGAN NAME -------------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- STA ------ BRAIN LIVER IOLRII 2-IOSS 17.0301 4169 % 3'3123 % 1.0000 1.0884 WEIGHT WEIGHT LUNG ILU) LF. TESTISS 0ITEO ISO. 1.7292 1.7848 .3424 % .3S34 % .0213 .8476 WEIGHT WEIGHT RT. TESTI TE 1.1211 .3600 X .8649 WEIGHT -------AC-C-ES-S-/-SE-X-----0-------------------3-.6-0-5-0-------------------.-71-3-0--% --------------1-.-71-2-2------------W-E-IG-H-T---------------- ORGAN NAME P A T N 0 L 0 0 V 0 9 S f ItV A T 1 0 N S KEYWORDS. ;EV'ERITY, DISTRIBUTION GROSS FREE-TEXT COMMENT; */& HISTOPATHOLOGIC Ft ----------------------------------------------------------------------------------------------------------- GENERAL COMMENT c LIVER (LIJ GI(OI.SI.:-A.L@. i.li.S6.Ei./6R.dA.iS.'N.bl.ti4.A@. . . . . . . . . 141@RO: -MINIMAL. FOCAL -IhFiLiRATiS*M6t46N6CiEAft'CiLi THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - BRAIN (OR). COAGULATING OLD (CO). LF. TESTIS ITEO), LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE ISVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZTETON LABORATORIES AMERICA, INC. 14ADISON, WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67551 DATE OF DEATH: 01/23/91 SEX: MALE D3OSE GROUP: 2 STUDY DAY OF DEATH: 5 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACK DATE AND TIME OF NECROPSY: (23/91 8:20 STUDY WEEK OF DEATH: S PROSECTOR: JULIE A. LARSEN TERMINAL 6ODY WEIGHT: 433.7 POST-FIX WEIGHER: NOT AVAILAoSl E PATHOLOGIST: ON. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-IG-H-E-R-: --G-A-I-L --t4-.-N-O-VA-K------------------- ORGAN NAt4E ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 N0 A ---------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- STA T -------- BLIRVAEIRN JLOINJ 2.1439 17.5586 4943 % 1.0000 WEIGHT T LUNG (LU) 1.0351 4.*0488 % .3772 % 8.1900 .7630 WEIGHT T LF. TESTIS OITEO NT. TESTIf.S TEI 1.6699 .3850 % .7719 WEIGHT T WEIGHT T ACCESS/S X 0 isol 1.0140 2.634S .3721 % .6074 % .7528 WEIGHT T -------------------------------------------------------------------------------1-.2-2-8-1------------W-E-IG-H-T --T---------------- OROAN NAME P A T H 0 L 0 0 Y* 0 0 S E A V A T 1 0 N S KEYWORDS. ;EAVEaRITY. DISTRIBUTION Ob a a ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND GENERAL COMMENT ------------------------------------------------- LIVER ILI) GI(OICSI-:AL@ iliS6Ei/6*6AiS*N6Ri4Ai INFTAMMATION.GRANULONATOUS THE- FO-LLO-WING- T-ISS-UES - WE-RE -UNR-EMARw KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - BRAIN (DR). COAGULATING OLD (CGI. LF. TESTIS (TEO). LUNG (LU), PROSTATE (PA). SEMINAL VESICLE (SVI, URETHRA I --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- AN104AL NUMBER: C67559 SEX: 14ALE DOSE GROUP: 2 DATE Of DEATH: 0142S/91 DATE AND TIME OF ECROPSY: STUDY DAY OF DEATH: 37 /25/91 7:58 SACRIFICE STATUS: SC EDULED FIRST INTERIM SAC STUDY WEEK Of DEATH: 6 TENRMINAL 6ODY WEIGHT: 401. POST-FIX WEIGHER: NOT AVAILAoBLlE PROSECTOlt: DENNIS HOFFMAN PATHOLOGIST: DR. TOM PALMER RECORDER: NANCY DIEDRICH --------------------------------------------------------------------------------W-EI-G-H-ER-:---P-AT-H-O-LO-G-Y---DE-P-A-R ---------------- ORGAN NAME ABSOLUTE ORGAN WEIOHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 na ---------------------BRAIN JOR) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- STA T ------- LIVER ILI) LUNG ILU) 2.OS13 11-2277 1.6181 .4200 % 3.7321 1 1.0000 8.1159 WEIGHT WEIGHT LF. TESTIS TE 1.5783 .3313 79 .7111 WEIGHT RT. TESTIS TEI0 1.5797 .3232 % .7604 WEIGHT ------A-C-C-ES-S-/S-E-X---0-1-(-i--I-SO-)--------------3-.-SS-8--4----- .3234 % .7286 % .7101 1.7347 WEIGHT WEIGHT - - - - - - - - - - ------ ---- - - - - --- ORGAN NAME P A IH 0 L 00 Y KEYWORDS. ;E*VERITY. DISTRIBUTION 08 SE N V A T 1 0N S -------------------- ----------------------------------------------------------G-RO-S-S FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FIN GENERAL COMMENT c ------------------------------------------------ GI(OISI:-ALi iliS6Ei/6R6AiS'N6R*MAL' . . .. ... . .. .. .. . . THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNN-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - UBRREATI"NR JOR()U.R)COAGULATING GLD ICO). LF. TESTIS (TEO). LIVER ILIP. LUNG FLUI. PROSTATE (PRI. SEMINAL VESICLE ISV --------------------------------------------------------------------------------------------------------------------- APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67SOO DATE OF DEATH: 02412/91 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE AND TIME OF STUDY DAY OF DEATH: 5S ECROPSY: 02(12/91 1:22 PROSECTOR: STUDY WEEK OF DEATH: RUTH SCHNITZ I TERMINAL 6ODY WEIGHT: 472. POST-FIX WEIGHER: NOT AVAILA13 E PATHOLOGIST: DR. TOM PALMER RECORDER: DENNIS HOFFMAN ---------------------------------------------------------------------------W-E-IO-H-ER-:---P-A-TH-O-LO-G-Y---DE-P-A-R --------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (ORAMS) ORGAN WEIO"T RELATIVE OROAN TO BRAIN 0 p0 --------------------- -------- TO BODY WEIO"T -------- WEIOHT RATIO -------- S--T--A--T- BRAIN IBRI LIVER ILII 2.0403 .4322 % 1.0000 WEIGHT LUNG ILU) LF. TESTIS OITEOI 17.4734 1.5854 I.S902 3.7012 % .33S$ % .3361 % i.se4i .7770 .7794 WEIGHT WEIGHT RT. TESTIS ACCESS/SEX TEL a ISO) I.S396 .3201 % .7S46 WEIGHT WEIO"T I -------------------------------------3.-6-8-0-6-------------------.7-7-9-6--% --------------1-.8-0-4-0------------W-E-IG-H-T--T---------------- ORGAN NAME P A T H 0 L 0 0 V 0 B S E R V A I 10 N S KEYWORDS, ;EAVER*ITY. DISTRIBUTION OE-N-ER-A-L--C-O-MM-E-N-T----IG-C-I-: ---------------------------------------O--R--OS--$---FR-E-E--T-E-XT---C-O-M-ME-N-TS-------HI-S-T-OP-A-T-HO-L-O-GI-C----F-IN-D------- OROS -AL@ ilS6Ei/bltdAiS'N61titAi THE- FO-LLO-WING- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON-.- - - - - - - - - - - - - - BURRAEITNHRAIBR(IU.N) COAGULATING GLD ICUI, LF. TESTIS ITEO), LIVER ILI). LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE (SV --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67561 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST POST-RECO DATE OF DEATH: STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 DATE AND TIME OF ECROPSY: 05 17/91 8:09 PROSECTON: DEBBIE PINKEL TEITMINAL 6ODY WFIGHT: $12 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE WEIGHER: JACK SERFORY -------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT (t) ORGAN TO BRAIN WEIGHT RATIO 0 RG --------------------- -------- -------- -------- S TA T ------- BRAIN JLOINJ LIVER 2.2294 10.6096 3831 % 3.'OISI % 1.0000 8.4119 WEIGHT LUNG ILU) ED 1.5853 .2S$7 IL .7111 WEIGHT WEIGHT LF. TESTIS 1 1.3128 .2142 % .5189 WEIGHT OT. TE:STIS ITTE ACCESS/SEX ORO ISO) 1.6140 4.2129 .2741 Z .6175 % IS54 1.8897 WEIGHT ------------------------------------------------------------------------------------------------WE-I-GH-T----------------- ORGAN NAME P A T H 0 L 0 0 Y- 0 0 S E P V A T 1 0 N S 1 0 KEYWORDS. ;EVaER*ITY, DISTRIBUTION ----------------------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/-%--HI-S-T-O-PA-T-H-OL-O-G-IC----FI-N------- GENERAL COMMENT AGCI: os LF. TESTIS (TEO) -AL@ iliS6Ei/61tdAiS NORMA E MICRO: I;ObEiAiE,' MULTI-FOCAL -ATROP"Y/DEGENERATION THE- FO-LLO-WING- -TISS-UES- WE-RE -Ut4-REMA-PtK-ABLE- AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON-.- - - - - - -------B-R-A-I-N--I-O-R)-.--C-O-AG-U-L-A-TI-N-G--O-L-D--(-C-GI-,--L-I-V-ER---(L-I-)-.-L-U-N-G--(-L-U)-.--P-R-O-ST-A-T-E--(-P-R-I.--S-E-N-TN-A-L--V-E-S-IC-L-E---(S-V-I-,-U-R-E-TH-R-A ---(U-R-I----------------- tn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67562 SIX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED. FIRST INTERIM SACR DATE OF DEAIH:,0F1425491 STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 DATE AND TIME EC OPSY: 01(25191 1:28 PROSECTON: JULIE A. LARSEN POST-FIX WEIGHER: NOT AVAILAS E TE RMINAL BODY WEIGHT: 433.8 RECORDER: JILL BEVAN ------------------------------------------P-AT-H-O-L-OG-I-S-T:---0-4-.--T-OM--P-A-L-ME-R----------------W-E-I-GH-E-R:---P-A-T-HO-L-OG-Y---D-EP-A-R-T ---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN WEIGHT RATIO 0 R0 A ---------------------- -------- -------- -------- S TA T -------- BRAIN JLORli LIVER 2.0610 .4751 % 1.0000 WEIGHT T LUNG ILU) LF. TESTIS 19.3861 1,4976 1.7701 4.4019 % .3452 % .4010 % 9.4002 .7266 .1589 WEIGHT T WEIGHT T WEIGHT T RY. TESTIS TE 1.7361 .4002 % ------A-C-CE-S-S-/S-E-X---O-IT-G-EO-I-ls-o-l--------------3.-1-2-7-$-------------------.-72-10 % .8424 1.5170 WEIGNI T WEIGHT T I------------------------------------------------------ a a a P A T H 0 L 0 0 Y 0 8 S E R V A T 1 0 N S a * ok O-R-G-AN---NA-M-E-------------K-E-Y-WO-R-D-S,---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND ------------------------------------------------- GENERAL COMMENT c LIVER ILI) GAGOSI: -ALi iliS6Ei/bRdAkS*N6RkA@ HYPENTR.OP."Y. . ".EP.ATO.CE.LL.ULAR: CHARACTERIZED BY AN CEt4TRILOSULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE "PLUMP' APPEARANCE THIS 'ENLARGED" APPEARANCE, THE CELLS ARE OTHERWII THE- FO-LLO-WING- T-ISS-UES-WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - - BRAIN (BRI, COAGULATING GLD (CO), LF. TESTIS ITEO). LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE (SVI, URETHRA (U ---------------------------------------------------------------------------------------------------------------------- C) 0% INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORA70RIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL HUM13ER: C67563 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE DATE OF DEATH; 03/22/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 (22/91 1:01 PROSECTOR: KEVIN BILLINGS TERMINAL 6ODY WEIGHT: $31.1 POST-FIX WEIGHER: NOT AVAIL203 E PATHOLOGIST: DR. T014 PALMER RECORDER; JOE N. KIZZIEE WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I ORGAN TO BRAIN WEIGHT RATIO 0 R0A S TA T ---------------------- -------- -------- -------- -------- BRAIN (OR) 2.1397 .3387 % 1.0000 WEIGHT T LIVER ILI) 26.7202 4.2299 % 12.4871 WEIGHT 7 LUNG (LU) LF. TESTIS OITEOIISOI RT. TESTIS TE 1.8608 1.1004 1.7096 .2946 % .2$SO % .2700 % .1697 .8414 .7990 WEIGHT t WEIGHT T WEIGHT T ACCESS/SEX 0 3.8080 .8123 % 1.1077 WEIGHT T --------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 a Y' 0 8 S E N V A T 1 0 N S a a a KEYWORDS. S*EVAER*ITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND -------------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER ILI) GA(OICSI:-iL@ iliS6Ei/6R6AiS*N6RP4A@ . .. .. ... .. . .. .. .. . .. .. .. . MICRO: -MINIMAL -NiPiRilt6P@Y' 'HE*PA'TOC*EL*L'ULA*R''C:"'AR*AC'TE*R'IZE*D'BA'NY CENTRILOBULATED PATTERN IN WHIC14 THE "EPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL @ORDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP* APPEARANC THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTHERWI THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - BRAIN ION). COAGULATING OLD ICO). LF. TESTIS (TEO). LUNG (LUI. PROSTATE IPRI, SEMINAL VESICLE (SVI. URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U,S.A. PR --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C07564 DATE OF DEATH: 03/25/91 SEX: MALE DgoOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE STUDY DAY OF DEATH: DATE AND TIME OF NECROPSY: 03/25/91 9:14 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT:. POST-FIX WEIGHER: NOT AVAILA13LE PROSECTON: JULIE A. LARSEN PATHOLOGIST: DR. RECORDER: JOE N KIZ'zIE --------------------------------------------------------------TO-M--PALMER ABSOLUTE ORGAN WEIGHT --------------------------W-E-IG-H-E-R:----S-TE-V-E--V-A-N--A-D-ES-T-I---- ORGAN NAME ---------------------- (GRAMS) ORGAN WEIGNT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 R0A BRAIN -------- -------- WEIGHT RATIO S TA T 2.1277 -------- -------- LIVER IOLRII LUNG ILU) 20.0061 1.5698 .3733 % 3.SI05 % .275S 2 1.0000 9.4027 .7370 WEIGHT T WEIGHT T LF. TESTIS T RT. TESTIS OIT'EIOISO, 1.8240 1.7241 .3201 % .$S?3 WEIGHT T ACCESS/SEX a 3.9433 .3025 X .1103 WEIGHT T WEIGHT T -----------------------------------------------------------------.-89-1-9--%----------------I.-I-S-3-3-------------W-E-IG-H-T---T------- ORGAN NAME ---------------------- KEYWORDS, ;EVaERI*TY. P A T "0 L0 DISTRIBUTION 0 Y' 0 8 SE R VA T 10 NS ---------------------------------------GROSS FREE-TEXT COMMENT; */ A HISTOPAT"OLOGIC FIND GENERAL COMMENT ------------------------------------------------- LIVER ILI) 04'OCSI: -iL@ iliS6Ei/61tdAiS'N6RitAi . .. . .. .. .. . A16R6:'-AlilAAi,'H6Lil'F6CAL' MINIMAL '* :IiFiLiftATiS:M'ON*ON'UCL*EA'R'CiLL' -"YPERTROP"Y "EPATOCELLULAR: CHARACTERIZED BY AN CENTRILOSULATED PATTERN IN WHICH THE "EPATOCYTES A AITOMUANEDED HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ING THE CELLS A MORE "PLUMP' APPEARANCE THIS_'ENLIGNVOED" APPEARANCE. THE CELLS ARE OTHERWIS - -THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - --- --- -- ---- - BRAIN (BRI. COAGULATING GLD (CGI. LF. TESTIS (TEO). LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE ISVI. URETHRA fU ---------------------------------------------------------------------------------------------------------------------- cc INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C6756S SIX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA DATE Of DEATH: 01/2S/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 37 STUDY WEEK Of DEATH: 6 (2S/91 $:SO PROSECTON: NANCY DIEDRICH TERMINAL DOOV WEIGHT: 290 RECORDER: JILL BEVAN P-O-S-T--F-I-X --W-EI-G-H-ER-:---NO-T--A-VA-I-L-AoB-l--E----------------P-AT-H-O-LO-G-I-ST-:---D-R.---TO-M--P-A-LM-E-R----------------W-EI-G-H-ER-:---P-AT-H-O-LO-G-Y---DE-P-A---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ita -------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA ------ BRAIN 0 1.9776 .5066 x 1.0000 WEIGHT LIVER Ll 16.3602 4.1906 % 1.2728 WEIGHT LUNG (LU) 1.4864 .3807 % .7516 WEIGHT LF. TESTIS TEO RT. TESTIS 0 1[1 1.5171 1.5533 .3818 % .3979 % .76?S .7054 WEIGHT ACCESS/SEX IQ ISO) 2.2069 SGS3 % I.IIS9 WEIGHT WEIGHT ------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T " 0 L 0 0 Y' 0 0 S E A V A T 1 0 N S KEYWORDS. ;E*VERITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-;--*-/-*--H-IS-T-O-PA-T-H-OL-O-G-IC----F-l ------ GENERAL COMMENT LIVER (LI) Ol'OOCSI-:ALi iliS6lEi/6lt6AiS'N6Ri4Ai . .... .. .. . .. .. .. .. .. . .. . . MICRO: -MINIMAL. FOCAL -liFiLiRATiS'.M6N6N6Ci-EAR*CiLi -MINIMAL -HYPEITTROPHY "EPATOCELLULAR: C14ARACTERIZED BY A CENTRILOSULATED PATTERN IN WHICH THE HEPATOCYTEI A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE *PLUMP" APPEARAI THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTHEM THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON.-- - - - - - - - - - - - - - - BRAIN IBRI, COAGULATING GLD (CG). LF. TESTIS (TEO). LUNG (LUP. PROSTATE (PR), SEMINAL VESICLE (SVI. URETHRA ---------------------------------------------------------------------------------I---------------------------------- to INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67566 SEX: MALE DOSE GROUP: 3 DATE OF DEATH: 01/24/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 36 01 24/91 9:14 PROSECTOR: SACRIFICE STATUS: STUDY WEEK OF DEATH: 6 KEVIN BILLINGS SCHEDULED FIRST INTERIM SAC TERMINAL 6ODY WEIGHT: 421. RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER ---------------------------------------------------------------------------------------W-E-IG-H-E-R:----D-EB-B-I-E --P-I-N-K-EL---------- ORGAN NAME ABSOLUTE ORGAN WEIGHT OROAN WEIGHT RELATIVE ORGAN TO BRAIN 0a0 ---------------------- (GRAMSI -------- TO BODY WEIGHT (11 -------- WEIGHT RATIO -------- S TA T BRAIN (LOIRJ LIVER I 2.1609932 .5002 % 1.0000 ------WEIGHT LUNG ILUP Is. 1 1.47s6 4.6701 % 9.3371 WEIGHT LF. TESTIS $tT. TESTIS ITEO1 ITE 1.7004 1.7142 .3499 % .4051 1 .4065 % .6996 .8100 .8127 WEIGHT WEIGHT WEIGHT ACCESS/SEX 0 0 ISO) 2.8099 .6189 % 1.2374 -------------------------------------------------------------------------------------------------------W-E-I-GH-T--------- ORGAN NAME KEYWORDS. ; SE RV A T10 N S ---------------------- -----------E-VE-R-I-T-Y.---D-I-S-TR-I-B-UT-I-O-N-----------GROSS FREE-TEXT COMENTS HISTOPATHOLOGIC FIN GENERAL COMMENT G AOOCSI : -A.LL. T.IS.SUE.S/.OR.GAN.S . .NO.RMA.L . . . . . "* . . .--.-.--.--.--.--.--.--.--.-----------.--.--.--.--.--.--.-.--.----- LIVER ILI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -ml THAL. MULTI-FOCAL -MIN"114AL -INFILTRATES.MONONUCLEAR CELL -"YPERTROP"Y HEPATOCELLULAR: CHARACTERIZED BY At CENTITILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MNE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANC THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTHERWI THE- FO-LLOW-ING - -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN (SRI. COAGULATING GLD (CGO. LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA I -------- ------------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67S07 DATE Of DEATH: SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST POST-RECO DATE AND TIME OFOS41E6C/R9O1PSY: STUDY DAY OF DEATH: 148 STUDY WEEK OF DEATH: 22 PROSECTOR: DEBBIE PIRKEL TEITHINAL 600Y WEIGHT: 640 POST-FIX WEIGHER: NOT AVAILAOSS(Ele/91 1:09 PATHOLOGIST: DR. TOM PALMER RECORDER: JOE M. KIZZIE ---------------------------------------------------------------------------------WE-I-GH-E-R-:---JA-C-K--S-ER-F-O-RT------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSO ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra -------------------- -------- TO BODY WEIO"T (%I -------- WEIGHT RATIO -------- S IA ------ BRAIN JLORil LIVER LUNG (LU) 2.2313 11.9256 1.6677 .3641 % 2.$SS? % .260S X 1.0000 8.1180 IIS4 WEIGHT WEIGHT WEIGHT LF. TESTIS TEO NT. TESTIS tEl 1.7147 1.1164 .2781 2 .2131 2 .1655 .7791 WEIGHT ------A-C-CE-S-S-/S-E-X---0-1-0---IS-O-)--------------3-.-2$-8-0--------------------sl-3-s--x--------------1-.-41-0-4------------WW-EE-IIOG-HH-TT----------------- OROAN NAME P A T H 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S 4 6 A KEYWORDS, ;EOVER*ITY. DISTRIBUTION -GE-N-ER-A-L ---CO-M-ME-N-T ----------------------------------------------G--R--O-S-S---FR-E-E---TE-X-T---CO-M-M-EN-T-S---/---H-IS-T-O-P-AT-H-O-LO-G-I-C---F-IN-------- LIVER ILI) GI(OICSI:-ALL iliS6Ei/6RdAkS'Pi6Rl4A@ . ... ... ... ... .. .. . .. .. . .. .. . .. .. .. . .. .. . .. .. . ... .. MICRO: -MINIMAL, FOCAL -INFILTRATES.MONONUCLEAR CELL THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - BRAIN JONI, COAGULATING OLD (COI, LF. TESTIS (TEOI. LUNG ILU). PROSTATE IPRI. SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. pp -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CB?568 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDU0L,ED FIRST INTERIM SAC DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 36 DATE AND TIME OF NECROPSY: 01/24/91 7:55 STUDY WEEK OF DEATH: 6 TEItHl AL 6ODY WEIGHT: 397. PROSECTOlt: KEVIN BILLINGS RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: ON. TOM PALMER WEIGHER: DEBBIE PINKEL -------------------------------------------------------------------------------------------------------------------- ORGAN NAME --------------------- ABSOLUTE ORGAN WEIGHT JORAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) OROAN TO BRAIN WEIGHT RATIO 0 Ra S TA 7 -------- -------- -------- ------- BRAIN JLOIRJ LIVER 1?'..:g4o01 .4988 x 1.0000 WEIOHT LUNG ILU) 1.3807 4.5180 % .3493 % 0.0602 .7003 WEIGHT WEIOHT LF. TESTIS .'ITTEJIIS., R7. TESTIS I I.SI31 I.S234 .3813 % .3837 % .7645 WEIGHT ACCESS/SEX 0 2.9710 .7484 % .7694 1.5004 WEIGHT 7 WEIGHT T --------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 0 Y 0 0 S E N V A T 10 N S a A a KEYWORDS. ;EOVERITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-O-PA-T-"-OL-O-G-IC----FI-N-D------- OEMENAL COMMENT LIVER (LI) 10@OOCSI-:ALi iliS6Ei/6ft6AiS*N6R'NA'L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .... . .. .. .. .. .. .. .. . .. .. . .. .. MICRO: -MINIMAL -"YPENTROP "EPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBUL""ATE D PATTERN IN W141CH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANC - - - - - - - THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTHERWI THE F0 LLOW I140 TISSUES WERE -UNREMARKABLE A-T MICROSCOPIC EXAMINAT ION-: - - - - - - - - - - - - - - -------B-R-A-I-N--I-D-R-).--C-O-A-GU-L-A-T-IN-G--O-L-D--I-C-O-I.--L-F-.--TE-S-T-I-S--(-T-EO-I-.--L-UN-G--(-L-U-I-.--PR-O-S-T-AT-E---(P-R-I-.--S-EM-I-N-A-L -V-E-S-I-CL-E---(-SV-I-.--U-RE-T-H-R-A--I------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: COIS69 SEX: MALE DOSE GROUP: 3 DATE OF DEATH: 01/24/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 36 (24/01 7:29 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA STUDY WEEK OF DEATH; 6 TERMINAL 6ODY WEIGHT: 319 POST-FIX WEIOHER: NOT AVAILAoBl E PROSECTOR: JULIE A. LARSEN RECORDER: VAL QUINN ------------------------------------------PA-T-H-OL-O-G-IS-T-:---D-R.---TO-M--P-A-L1-4-EN----------------W-E-IG-H-E-R:---D-EB-B-I-E --P-I-NK-E-L----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (ONAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ---------------------BRAIN (041 -------- TO BODY WEIGIII -------- WEIGHT RATIO -------- S TA LIVER (LI) 2.0620 16.0008 .5290 % 1.0000 LUNO ILU) LF. TESTIS T NY. TESTIS TEI 1,3330 1.7269 1.7674 4.1049 % .3420 % .4430 % 7.7598 .646S .8375 WEIGHT WEIGHT WEIGHT ACCESS/SEX 0 a Sol 2.6638 .4534 % .0134 % .$sit WEIGHT -------------------------------------------------------------------------------1.-2-9-10-------------WE-I-G-HT------------------ O-R-G-AN---NA-M-E-------------K-EYWORDS. ; IN0 P A T N 0 L 0 G Y' 0 8 S E A V A T 1 0 N S a a a EVERITY. DISTRIBUTION ------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC Ff GENERAL COMMENT ------------------------------------------------ LIVER ILI) GIOOCSI:-ALi iliS6Ei/61tdAiS'N6Rk@ . . . . :' * . * '' '* ** * *. .. .. . .. .. ... . 1416lt6':-AlilkAi. . . . . -.IN.FL.AI@.MATiOi.6RAN6L6MAT6ui THE FOLLOWING TISSUES WERE-UNREMARNABLE AT MICROSCOPIC EXAMINATION: - - - - - - - - - - - - - - -------B-R-A-I-N --(-OR-)-.--CO-A-G-U-L-AT-I-N-G--O-L-D--(-CO-I-.--LF-.--T-E-S-T-IS---(T-E-O-)-.--LU-N-G--I-L-UI-.--P-R-O-ST-A-T-E--I-P-R-I.--S-E-M-I-NA-L--V-E-S-IC-L-E--(-S-V-I-.-U-R-ET-H-RA------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMCRICA, INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C67570 DATE Of DEATH: 024EI4/91 SEX: MALE DOSE GROUP: 3 STUDY DAY OF DEATH: 57 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SO DATE AND TIME OF CROPSY: 02(14/91 7:40 STUDY WEEK OF DEATH: 9 PROSECTOR; RUT" SCHNITZ TERMI NAL 6ODY WEIGHT: 442. POST-FIX WEIGHER: NOT AVAILAS E PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-I-GH-E-R-: --V-A-L--Q-UI-N-N--------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 Ra ---------------------BRAIN JOLNli LIVER -------2.2485 18.0879 -------SO79 % 4.0851 % WEIGHT RATIO -------- 1.0000 8.0444 S TA T ------WEIGHT LUNG ILU) LF. TESTIS ITEO 1.4029 1.8392 .3169 % .0239 WEIGHT WEIGHT NT. TESTIS TEIISO) 1.7892 .4155 % .4042 % .1180 .79S7 WEIGHT -------AC-C-ES-S-/-SE-X---o-i-a-------------------3-.9-6-6-1-------------:------I-O-S9 % 1.7639 WEIGHT WEIGHT ------------------------------------------------------ O-R-G-A-N--N-A-ME-------------K-EYWORDS. PA T"0 L0 0 Y ;EAVEARITY, DISTRIBUTION 0 0 S E N V A T 10 N S A 4 A GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC Ft OENERAL COMMENT IGC --------------------------------------------------------------------------------------- LIVER ILI) GROSI: -kAi&C@Uil6N* . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . M161tb:'-i4lili4A@ -HYPENTROP"Y "EPATOCELLULAR: CHAR.ACT.ER.IZ.ED. .BY. A. CENTRILOSULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAN THIS 'ENLARGED" APPEARANCE. THE CELLS ARE OTHERW - -THE- FO-LLO-WING- -TISS-UES -WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - 7 - - - - - - - -------B-R-A-IN--I-B-R-)-.--CO-A-G-ULATING OLD (CO). LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE (SVI, URETHRA ---------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67571 DATE OF DEATH: 03/22/91 SEX: MALE DOSE GROUP: 3 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 93 03(22/91 7:41 STUDY WEEK PitOSECTORI RUT" OFSACDREIAFTHI:CE 1S4TATUS: SCHEDULED TERMINAL SACRIFI( TERM114AL 6ODY WEIGHT: 491 POST-Flx WEIGHER: NOT AVAILAO E SCHNITZ ------------------------------------------P-A-TH-O-L-OO- S:T: DR. TON PALMER R ECORDER: JOE N. KIZZIEE ---------------------------------------WE-I-G-H-ER-:---JI-L-L--B-E-VA-N-------------- ORGAN NAME -------------------- ABSOLUTE ORGAN WEIGHT _!GRAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT III ORGAN TO BRAIN 0 Ra BRAIN SRI 2.12SS -------- WEIGHT RATIO -------- S TA T ------- LIVER ILI LUNG LU11 17@3730 .4324 % 3.5340 % 1.0000 WEIGHT LF. TEIT TEO11 1.4238 1.9603 .2890 % 1.1736 .6699 WEIGHT WEIGHT AT. TEST s ITE ACCESS/SEX' 040 ISOI 1.8607 .3988 % .3715 % .9223 WEIGHT -------------------------------------4-.-29-9-2--------------------17-4-5--%---- IYS4 2.0227 WEIGHT ------------------------------------W-E-IG-H-T-------- O-R-GA-N---NA-M-E--------------KEYWORDS, SEVERITY. P ADISTTRNIB0UTILON0 0 r 0 8 S E N V A T 1 0 N S a * a GENERAL COMMENT JOCI ------------------------------------G--R--O-S-S---FR-E-E--TEXT COMMENTS / HISTOPATHOLOGIC FIN LIVER ILI) 0 OS : -ALL TISSUES/ORdAiS.N6ltilA@ . . . -.-.--.--.--.-.--.--.--.-.--.--.--.-.--.--.--.-.--.--.----- A16R6:'-m NINAL.'FOCAL' -MIINIMAL . . . . . . . . . :.IN.FIL.TR.AT.ES,.MO.NON.UC.LE.AR-.CE.LL - - . . . . . . . . . . HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED BY A CENTAILOBULATEI) PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDEP GIVING THE CELLS A MORE 'PLUMP" APPEARAN THIS ' NLARGED' APPEARANCE. THE CELLS ARE OTHERW THE FOLLOWING TISSUES WERE-UNREMARK-ABLE- - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON ---- - - - - - - - - - - - - - -------B-R-A-I-N --I-SR-)-.--CO-A-G-U-L-AT-I-N-G--G-L-C-)-(-C(-i-l-.-L-F.--T-E-S-T-IS---(T-E-O-I-.--LU-N-G--(-L-U-I.--P-R-O-S-TA-T-E--(-P-R-I-,-S-E-M-I-NA-L--V-E-S-IC-L-E--I-S-V-I-.--UR-E-T-H-R-A ----------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: Ce?572 SIX: MALE DOSE GROUP: 3 DATE OF DEATH: OS417191 STUDY DAY OF DEATH: 149 DATE AND TIME OF ECROPSY: OS 17/91 7:18 SACRIFICE STATUS: SCHEDULED. FIRST POST-RECOV STUDY WEEK OF DEATH: 22 TE RMINAL BODY WEIGHT: 553. POST-FIX WEIGHER: NOT AVAILAD(E POTOSECTOR: DEBBIE PIRKEL PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE --------------------------------------------------------------------------------W-E-IG-H-E-R:---J-A-C-K --S-ER-F-OR-T------------------- ORGAN NAME ABSOLUTE ORGAN WIEIGHT ORGAN WEIGHT RELATIVE ---------------------- (GRAMS) -------- TO BODY WEIGHT (%) -------- BRAIN (80) LIVER (LI) LUNG ILU) 2.22SI 18.404S .4022 % 3.3263 % LF. TESTIS EO1 1.7sis 1.6489 .3108 % RT. TESTIS TE ACCESS/SEX 0 1.5651 .2980 % .2829 % ---------------------IS-O-)--------------3--.--3--0-2--3----------------------s--o--o-t- %- ORGAN TO BRAIN WEIGHT RATIO -------- 1.0000 8.2713 .7172 .7410 .7034 1.4841 0R a STA T ------- WEIGHT WEIGHT WEIGHT WEIGHT WEIGHT WEIGHT O-R-GA-N---NA-M-E-------------K-EYWORDS, P A T N 0 L 0 0 Y * 0 0 S E P V A T 1 0 N S------&--,--------------------;EAVERITY. DISTRIBUTION ------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPAT"OLOAIC FIN GENERAL COMMENT AOCI . . . . ..... .--.-.--.--.--.-.--.--.-.--.--.--.-.--.--.--.--.-.--.--.--.-.--.--.------ LUNG ILU) G OS : -ALL TISSUES/ORGANS NOR14AL THE FOLLOWING TISSUE-S WEItE-UNRE14ARKABLE NECROPSY NOTE: )TRIMMIN(I COMMENT: TWO LtFi iOiEi 6F'L6Nd iO6N6 IN BOTH_PROCESSED. ONE IN BLOCK 42, AND ONE IN BLOCK AT MICR-OSCOPIC EXAMINATIO-- --- --- -- --- --- -------BU-RREATIHNRI- BR(IUi)COAGULATING OLD (COI, LF. TESTIS -------------------------i--- (TEO), LIVER (LIN)-.LUNG (LU), PROSTATE (PRI, SEMINAL VESICLE (SV ---------------------------------------------- Ch INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. pa -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7573 DATE OF DEATH: SEX: MALE I)OSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST POST-RECO% DATE AND TIME OFOS41E7C49O1PSY: STUDY DAY OF DEATH: 149 OS 17/91 7:40 PROSECTOR: STUDY WEEK OF DEATH: DEBBIE PINKEL 22 TER14INAL 6ODY WEIGHT: S2$. POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE -------------------------------------------------------------------------------W-E-IG-H-E-R:---J-A-C-K --SE-R-F-OR-T------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (ORAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 pa ----------------BRAIN JLOIRJ LIVER -------- 2.14S4 14.1228 -------.4073 % 2.6814 % WEIGHT RATIO -------1.0000 S TA T ------WEIGHT LUNG ILU) LF. TESTIS ITIO I S733 .2987 % 6.5121 .7333 WEIGHT WEIGHT MT. 11'.STIS TEI 1.7071 1.7022 .3241 % .7957 WEIGHT ------A-CCESS/SEX 0 ISO) 3.6831 .3232 % .69ss % .7934 1.7074 WEIGHT WEIGHT --------------------------------------------------------------------------------------------------------- ORGAN NAME P A I " 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S 1%* a KEYWORDS, ;E'VERATTY, DISTRIBUTION -----------------------------------------------------G-RO-S-S FREE-TEXT COMMENTS / HISTOPAT"OLOGIC Fl BRAIN (04) -----------------------------------------------. . . . . . . . . . . . . . . . . . . . . . ... . . . . . . . . . . . . GROSS: MICRO: -TISSUE MISSING NECITOPSY flOiE: -,TRIMMING COMMENT: MISiIAG'FiOA ilet fliS6E'Ai ilA GENERAL COMMENT c GI(OISI: -ALi iliSuEi/6R6AiS'N6*itAi . . . .. .. . .. . . .. .. . . THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - COAGULATING GLD ICO). LF. TESTIS ITEOP. LIVER ILI). LUNG (LUI, PROSTATE IPRI. SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?574 SIX: MALE DOSE GROUP: 3 DATE Or DEATH: DATE AND TIME OF0142E5C/R9O1PSY: STUDY DAY OF DEATH: 37 (25/91 1:22 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC STUDY WEEK OF DEATH: 6 TE MINAL 6ODY WEIGHT: 396. POST-FIX WEIGHER: NOT AVAILAoOl E PROSECTON: DENNIS HOFFMAN PATHOLOGIST: DR. TOM PAL14EN RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-EI-G-H-ER-:---P-AT-H-O-LO-G-Y---DE-P-A-R -------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSP ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 Ra ---------------------- -------- -------- WEIGHT RATIO -------- STA T ------- BRAIN ILO1R1 LIVER 2.0000 .5042 % 1.0000 WEIGHT LUNG (LU) ls.isio 1.4023 4.0703 1 .3534 % 8.0723 .7009 WEIGHT LF. TESTIS TE NT. TESTIS TEI0 1.8672 1.8099 .4706 % .9332 WEIGHT WEIGHT ACCESS/SEX 010 ISO) 3.4002 .4561 1 .8569 % .9046 1.0994 WEIGHT -----------------------------------------------------------------------------------------------W-EI-G-HT------------------ ORGAN NAME ; a * P A T H 0 L0 0 Y 0 0 S E A V A T 1 0 N S 0 A A KEYWORDS. EVERITY. DISTRIBUTION G-E-NE-R-AL---C-O-MM-E-NT------c------------------------------------!@-------- G-R-O-SS---FR-E-E--T-E-X-T --C-O-M-ME-N-T-S ---/---HI-S-TO-P-A-TH-O-L-OG-I-C---F-I-N-I ------ LIVER ILII 01'03sl:-iLi iliS6Ei/6R6AiS'N6NkAL MICRO: -MINIMAL. FOCAL .. ... .. . .. .. . .. . .. .. .. .. . -MINIMAL -INFILTRATES.MONONUCLEAR CELL -HYPEItTitOPHY HEPATOCELLULAR: . .. . .. ... . CHARACTERIZED BY A CENTITILOSULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLUMP* APPEARAN THIS "ENLARGED' APPEARANCE. THE- FO-LLO-WING- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - THE CELLS ARE OTHERW BRAIN (13R). COAGULATING GLD ICOI. LF. TESTIS ITEO). LUNG (LU), PROSTATE (PRI, SEMINAL VESICLE (SVI, URETHRA -------------------------------------------------------------------------------------------------------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67S75 SEX: 14ALE DOSE GROUP: 3 DATE OF DEATH: OS/16/91 STUDY DAY OF DEATH: 148 DATE AND TIME OF NECROPSY: OS 16/91 7:14 SACRIFICE STATUS: SCHEDULED FIRST POST-REC STUDY WEEK OF DEATH: 22 TERMINAL 600Y WEIGHT: 729 POST-FIX WEIGHER: NOT AVAILAB(E PROSECTOR: DEBBIE PINKEL RECORDER: JOE N. KIZZIE ------------------------------------------P-AT-H-O-LO-G-I-ST-:---D-R-. --T-OM--P-A-L-14-E-R---------------W-E-IG-H-E-R:---J-A-CK---SE-R-F-OR-T------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ----------------------- (GRAMSI -------- TO BOD-Y--W-EI-G-H-T- (%I WEIGHT RATIO -------- S TA BRAIN 16Lli LIVE LUNG ILU) 2.27S4 22.2S39 1.7949 .3120 % 3.OSII % .2461 % 1.0000 9.7102 ------ WEIGHT WEIGHT LF. TE TIS OITEO AT. TESTIS TE 2.0338 .2789 % .7881 .1931 WEIGHT WEIGHT ACCESS/SEX 0 (SO) 2.0447 3.4670 .2804 % .1980 WEIGHT ------------------------------------------------------------.4-7-S-5--% --------------1-.5-2-3-7------------W-E-IG-H-T ---------------- O-R-G-AN---N-AM-E-------------K-EYWORDS. P A T H 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S ;EV'ER'ITY. DISTRIBUTION GROSS FREE-TEXT COMMENT; &/a HISTOPATHOLOGIC Fl GENERAL COMMENT JOCI -------------------------------------------------------------------------------------. .. . .. . .. .. G OS : -ALL TISSUES/ORGANS NORMAL. . . . . ..... . . . . . . . . . . . . . . . . . . . . . . . . LIVER (LI) i4l6lt6:*-iLiGAT'iO@Ai -NECROSIS.COAGULATIVE - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON-.- - - - - - - - - - - - - - - - -------B-R-A-IN---(-ON-)-.--CO-A-G-U-L-AT-I-N-G--G-L-D --(C-G-O-.--L-F.--T-E-S-T-IS---(T-E-O-I-.-L-U-N-G--(-L-UI-.--P-R-O-ST-A-T-E--I-P-R-I.--S-E-M-IN-A-L--V-E-S-IC-L-E--(-S-V-I-, -U-R-E-T-HR-A----------------- INDIVIDUAL APPENDIX D ANIMAL PAT40LOOY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S A. PR -------------------------------------------------------------------------------------------------------------------- ADANIMAL NUMBER: C67576 TE OF DEATH- 01/23/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 3S 01(23/91 8.25 PROSECTOR: STUDY WEEK OF DEATH: DENNIS HOFFMAN 5 TERMINAL 6ODY WEIOHT: 387. P-O-S-T--F-I-X--W-E-IG-H-E-R-: --N-O-T--AV-A-I-LA-S--E----------------P-A-T"-O-L-O-(I-I-S-T:--D-R-.--T-O-M--PA-L-M-ER-----------------RE-C-O-RD-E-R-: --J-I-L-L--BE-V-A-N------------------- WEIGHER: GAIL M. NOVAK ORGAN NAME ABSOLUTE ORGAN WE143HT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 Ra -------------------BRAIN 1L8;l -------- -------- WEIGHT RATIO -------- STA T ------- LIVER 1.8902 17.4704 .4873 % 4.SO38 % 1.0000 0.2426 WEIGHT LUNG ILU) LF. TESTIS TE 1.3139 1.6117 .3387 % .69si WEIGHT WEIGHT PT. TESTIS OITEIOISO, ACCESS/SEX 0 1.6220 .4173 % .4181 % .8564 .8581 WEIGHT WEIGHT -------------------------------------3-.-18-1-1-------------------.-82-2-1--%--------------1-.-68-?-0-------------WE-I-GH-T----------------- O-R-GA-N---NA-M-E-------------K-EYWORDS, PA IH0 L0 0 Y ;E'VE4RITY, DISTRIBUTION 0 0 S IER V A T 1 0 N S 0 * A GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC FINC GENERAL COMMENT ------------------------------------------------- G400CSSI-:A-L-L-.-T-l-iS-6-l-Ei-/-i-)R-d-A-k-S*-N-6-Rk-E------------------- LIVER (LI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -MINIMAL . .. .. .. . . .. .. .. .. -"YPENTROPHY "EPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE *PLUP4P* APPEARANC THIS "ENLARGED* APPEARANCE, THE CELLS ARE OT"ERWI THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - -------B-R-A-I-N (ON). COAGULATING OLD ICO). LF. TESTIS (TEO). LUNO (LU). PROSTATE IPRI. SEMINAL VESICLE (SVI. URETHRA ---------------------------------------------------------------------------------------------------- APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. HADISDN, WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUM13ER: C67577 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA DATE OF DEATH: 01/23/91 STUDY DAY OF DEATH: 3S STUDY WEEK OF DEATH: S TERMINAL DODY WEIGHT: 363 DATE AND TIME OF NECROPSY: 01(23/91 9:46 POST-FIX WEIGHER: NOT AVAILAS E PROSECTON: JULIE A. LARSEN PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-I-GH-E-R-:--G-A-I-L --M.--N-O-V-AK----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra --------------------- -------- TO BODY WEIGHT 421 -------- WEIGHT RATIO -------- S TA ------ BRAIN JOLRli LIVER 2.0559 SGS4 % 1.0000 WEIGHT LUNG ILU) 16.0948 1.2149 4.426S % .3s34 % 7.1286 .02SO WEIGHT WEIGHT LF. TESTIS 'ITED) RT. TESTIS OITEII 1.5817 1.6657 .4350 % .4sol % .7693 .8102 WEIGHT ------A-C-CE-S-S-/S-E-X----G---I-S-O-)--------------2.-3-6-3-0-------------------.6-499 % 1.1494 WEIGHT WEIGHT ----------------------------------------------------- ORGAN NAME PA T"0 L0 0 Y KEYWORDS. ;E*VERITY.DISTRIBUTION -------------------------------------------------------- GENERAL COMMENT c 0 8 S E R V A T 1 0 N S INa a GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Ft ----------------------------------------------- LIVER ILI) 01'03.st.:-i.L@. f.li.S6.Ei./6.1td.Ai.S*.N6.RA.A@. . . . . . . . . M16RO: -MIN114AL -"ipift*TR,OPH,Y*.H*E*PA*TOC,EL*L,ULA*R*':CH*AR,AC,TE*R*IZE*D,B,YA, CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLUMP' APPEARAN THIS "ENLARGED" APPEARANCE, THE CELLS ARE OTHERW TH-EF-OLL-OW-IN-TGIS-SU-EWS-ERE-UN-RE-MAR-KA-BAL-TEM-ICR-OS-CO-PEI-XCAM-IN-AT-IO-N:- - - - - - r - - - - - - BRAINJOR).COAGULATIONLGD ICO).LF. TESTISITEO)L,UNGILU).PROSTATIEPRI.SEMINAVLESICLE(SVI.URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COIS?L DATE Of DEATH: 02/12/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED. SECOND INTERIM SA DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 55 STUDY WEEK OF DEATH: I (12/91 9:36 PROSECTON: RUTH SCHNITZ TERMINAL BODY WEIGHT: 466. POST-FIX WEIGHER: NOT AVAILA0B2 PATHOLOGIST: DR. TOM PALMER RECORDER: DENNIS HOFFMAN --------------------------------------------------------------------------------W-E-IG-H-ER-:---P-A-TH-O-L-OG-Y---D-EP-A-R---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0a a ---------------------- -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- STA T ------- BRAIN 8 LIVER ILRII 2.0477 .4389 % 1.0000 WEIGHT LUNG (LUI 17.5462 1.5098 3.7604 % .3236 % 2.5687 .7373 WEIGHT Lf. TESTIS .1,T1EO RT. TESTIS 1.774S 1.6661 .3103 % .1660 WEIGHT WEIGHT ACCESS/SEX 0 13 ISO) 3.2620 .3S?2 % .6991 % .8139 1.5930 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME PA IN0 L0 0 I(EYWORDS. ;EAVERITY, DISTRIBUTION 08 S ER VA T 10 NS *a a G-E-NE-R-AL---C-O-MM-E-NT-----------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-OP-A-T-HO-L-O-GI-C----FI-M------- GI(OICSI-:AL@ iliS6E@/6ft6AiS*N6Rki LUNG (LU) . . G.ROSS: . . .. .. .. . .. .. .. . .. .. .. .. .. . MICRO: -TISSUE MISSING 'NE'CN'O*PS,'YNO*TE*:)*TR*TM'M*INCG'*O'ME,N*T:'LUNG' iE6TiOA ittiSiNd ift6N'WiT' OF TRIMMING THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE AT MICROSCOPIC EXAMINATION: BRAIN (BRI. COAGULATING GLD ICOI, LF. TESTIS (TEOI. LIVER ILI), PROSTATE (PRI. SEMINAL VESICLE (SV). URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67S79 DATE Of DEATH: OS/16/91 SEX: 14ALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST POST-RECO DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 148 STUDY WEEK OF DEATH: 22 8:33 PROSECTOR:, DEBBIE PIRKEL TERMINAL 6ODY WEIGHT: 587 POST-FIX WEIGHER: NOT AVAILA0S5(Ele/91 PATHOLOO ST: DR. TOM PALMER RECORDER: JOE N. KIZZIE --------------------------------------------------------------------------------W-EI-G-HE-R-:---J-AC-K--S-E-RF-O-R-T------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R a -------------------BRAIN -------2.1446 TO BODY WEIGHT 1%) -------.3853 % WEIGHT RATIO -------1.0000 STA -----WEIGHT LIVER ILBRII LUNG ILU) 11.3980 1.6293 3.1342 % I.Slas WEIGHT LF. TESTIS 1.7570 .2770 % .2993 % .7s97 WEIGHT RT. TESTIS -1T1E1,? ACCESS/SEX 0 0 ISO) 1.7759 3.1273 .3025 % .0103 .8211 WEIGHT WEIGHT ------------------------------------------------------------O-S-20---% --------------1-.7-1-4-6------------W-E-IG-H-T ---------------- ORGAN NAME aA A PA T"0 L00 V 0 B SE R VA T10N S A A a KEYWORDS. SEVERITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-14-E-N-TS---/---"I-S-T-O-PA-T-H-OL-O-G-IC----F-l ------ GENERAL COMMENT LIVER (LI) . G.IG.OC.SI.:-A.LL. i.li.S6.E@./6.RdA.iS.'N.6R.kAi. . . . . . . . . . . . . . . . . . . . . . . . . . . .. .. .. .. .. .. MICRO: -SLIGHT. FOCAL -I$fFILTRATES.MONONUCLEAR CELL -- --- ------ -- ----- --- -- -- ---- --- --- --- - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: -------B-R-A-IN---(-80-)-.--CO-A-G-U-L-AT-I-N-G--G-L-D--(-CO-I-.--L-F.--T-E-S-T-IS---(T-E-O-P-.--LU-N-G--(-L-U-).--P-R-O-ST-A-T-E--I-P-R-I-. -S-E-M-IN-A-L--V-E-S-IC-L-E--(-S-V-I-,-U-R-E-T-HR-A---------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67510 SEX: 14ALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED. SECOND INTERIM SA DATE OF DEATH: 024,13/91 STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I DATE AND TIME OF CROPSY: 02 13/91 9:11 PROSECTOR: DENNIS HOFFMAN TERMINAL BODY WEIGHT: 441. POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOOIST: ON. TOM PALMER RECORDER: KEVIN BILLINGS WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------------------- OROAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN WEIGHT RATIO 0 Ra --------------------- -------- -------- -------- S TA T ------- BRAIN LIVER ILS*il 2.0126 4714 % 1.0000 WEIGHT LUNG ILU) 19.3210 I.S312 4:3732 % 9.2773 WEIGHT LF. TESTIS OITEOJISO) 1.0539 .3406 % .23$S % .73S2 .5001 WEIOHT WEIGHT RT. TESTIS If ACCESS/SEX G 1.5651 3.6804 .3543 % .7sis WEIGHT -----------------------------------------------------------------.8-3-3-0--%---------------1-.-7-6-72--------------W-EI-G-H-T ---------- A * 0 P A TH0 L0 0 Y 0 8S ER V A T10 N S 0 AA OROAN NAME ---------------------- KEYWORDS, SEVERITY. DISTRIBUTION ----------------------------------- GROSS FREE-TEXT COMMENTS HISTOPAT"OLOGIC LF. TESTIS (TEO 7 --- --------------- --------- FINE AROSi:*-iMAL@ MICRO: -MODERATELY SEVERE. DIFFUSE 10 X 11 .X .20. M.M .(F.OR COMPARISON RIGHT IS 10 X 13 -ATROPHY/DEGENERATION THE FOLLOW-ING - -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - BRAIN (OR). COAGULATING OLD ICGI, LIVER ILI). LUNG (LUP, PROSTATE (PR), SEMINAL VESICLE (SVI. URETHRA (URI ---------------m --------m ------------- ---------------M--M ------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67S$l DATE _OF DEATH: 02/13/91 SEX: 14ALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SAC DATE ANID TIME OF NEC70PSY: STUDY 02(13/91 DAY OF DEATH: 8:30 56 STUDY WEEK OF DEATH: I TERMINAL 6C)DY WEIGHT: 413.8 POST F X WEIGHER: NO AVAILAO E PROSECTOR: RUTH SCHMITZ PATHOLOOIST: DR. TOM PALMER I RECORDER: KIEVIN BILLINGS - ---------------------------------------------------------------------------W-E-I-GH-E-R-: --J--L-L--BE-V-A-N-------------------- OROAN NAME ABSOLUTE ORGAN WEIGHT JORAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R0 A --------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA I BRAIN (BRI LIVER ILI) 1.933S .3996 % 1.0000 -------I- LUNG,,ILU11 18.5770 1.7077 3.8398 % 9.6010 WEIGHT I WEIGHT LF. T (TEO) RT. TESTIS ITEII 1.7079 1.6983 .3s3o % .3s3o % .8132 .8833 WEIGHT I WEIGHT T ACCESS/ Sol 3.4546 .3sto % .1784 WEIGHT 7 ------------------------------------------------------------.-7-14-1--%--------------1-.-7-86-7-------------WE-I-GH-T---I-------------- ORGAN NAME P A T H 0 L 0 0 Y 0 8 S E R V A I 1 0 N S It ----------------------K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G-RO-S-S FREE-TEXT COMMENTS HISTOPATHOLOGIC FIND GENERAL COMMENT @GCI . . . . . . . . . . . . . . . . . .* ------------------------------------------------- LIVER (LI) G OSS: -ALL TISSUES/ORGANS NORMAL ... . . . . . . . . . . . . . . . . . . . . . . . . . i4l@it6:'-'Mlhli4A@ -*"'YPE'R*TNO'PH'Y''HE*PA'TOC'EL*LU*LAit:*C"*AR*AC*TE'R'lZiD'BiAN CENTIIILOBULATED PATTERN IN WHICH THE NEPATOCYTES A A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE "PLUMP" APPEARANCE - - - - - - - - - - - - - - - - - - - - - - - T-HIS -*EN-LAR-GED'- A-PPE-ARAN-CE,- T-HE -CEL-LS -ARE- OT-HER-WII THE FBORLALIONWIN(Gon)TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXA141HATION: -----------------.-C-O-AG-U-L-A-T-IN-G--G-L-D--(-C-GI-.--L-F-.--T-ES-T-I-S--(-T-EO-)-.--L-U-NG--(-L-U-)-.-P-R-O-S-T-AT-E--(-P-R-)-.--SE-M-I-N-A-L --VE-S-I-C-LE---I-SV-I-.--U-RE-T-H-R-A--(-U--------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C67582 DATE OF DEATH: 02/13/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I DATE AND TIME OF NECROPSY: 02(13/91 8:31 PROSECTON: DENNIS HOFFMAN TERMINAL 6ODY WEIGHT: 457. RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILAR E PATHOLOGIST: JULIE A. LARSEN WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 R0 ---------------------- -------- -------- WEIGHT RATIO -------- S TA T ------- BRAIN ION? LIVER LI) 2.1754 22.3lS2 .4757 % 1.0000 WEIGHT LONG ILU) 1.5590 4.8708 % .3409 % 10.2soo .7160 WEIGHT WEIGHT LF. TESTIS RT. TESTIS ITTEEOIJ 1.5470 .3313 % .7111 WEIGHT ACCESS/SEX 0 0 ISO) 1.5773 3.1744 .3449 % .6942 % .72SI 1.4S02 WEIGHT -----------------------------------------------------------------------------------------------W-E-IG-H-T----------------- ORGAN NAME P A T H 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S KEYWORDS. ;E*VERITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-;----/-*--HI-S-T-OP-A-T-H-OL-O-G-IC----F-IN-C------ GENERAL COMMENT IGCI: LIVER (LI) os -ALi il@S6E@/61tdAiS'N6Rki . .... .. .. .. MICRO: -SLIGHT. FOCAL -MINIMAL .. .. .. .. .. . .. .. . .. . .. ... .. . .. .. . .. .. . -INFILTRATES,MONOMUCLEAR CELL -HYPERTROPHY "EPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATEI) PATTERN IN WHICH THE HEPATOCYTES A 04ORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE "PLUMP" APPEARANC THIS 'ENLARGED" APPEARANCE,, THE CELLS ARE OTHERWI THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN (OR). COAGULATING GLD (CGI. LF. TESTIS ITEO). LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE ISVI, URETHRA I --------------------------------------------------------------------------------------------------------------------- Ch INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67583 SEX: MALE DOSE GROUP: 3 DATE OF DEATH- 02/12/91 STUDY DAY OF DEATH: 55 DATE AND TIME OF NECROPSY; 02 12/91 2-10 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA STUDY WEEK OF DEATH: I TERMINAL 6ODY WET NT: P-O-S-T--F-I-X --W-EI-G-H-ER-:---N-OT--A-V-AT-L-A-O--E-------------------PP-ART-OHS-OE-LCO-TGO-IR-S:T-:--KE-VD-IR-N.--BTOIMLLIPNAGLSMER 453. ECORDEft: DEN 1N0 IS HOFFMAN ABSOLUTEORGAN WEIGHT --- -------------ORGAN WEIGHT ------ -W-E-I-G-HE-R-:---P-A-TH-O-L-OG-Y---D-EP-AR ORGAN NA14E ---------------------- (()RAMS) -------- RELATIVE TO BODY WEIGHT OR2AN TO BRAIN W IGHT RATIO 0R a BRAIN 2.0247 -------- -------- S TA T ------- LIVER 11.8334 .4462 % 1.0000 WEIGHT LUNG LU11 LF. TELT 1.4036 4.IS02 % .3003 % 9.3011 WEIGHT IS ITIO AT. TEST TE 1.4211 .3133 % .6932 .7022 WEIGHT ACCESS/SEX 0 a 1.4119 3.5708 .3111 % .6973 WEIGHT WEIGHT ------------------------------------------------------------.7-8-6-9 % 1.7638 WEIGHT ORGAN NAME P A T H 0 L 0 0 V --0--8--S-E--R--V--A-T--1--0--N-S----a--a-a------------------------- ---------------------K--E-Y-WO-R-D-S,---;-E-IV-I-E-R*-I-TY-.-DI-S-T-RI-B-U-TI-O-N-----GENERAL COMMENT IGCI: GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIM LIVER ILII os -iLi iliS6Ei/6R6AiS'N6RAA@ ................................ MICRO: -AlililA@ -HYPENTROP14y' AEOAiO@EiL'LAR: 'CHARACTERIZ[O'BY A CENTRILOBULATED PATTENNUIN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERI ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANC - - - - - - - - - - - - THIS *ENLARGED- APPEARANCE. THE CELLS ARE OTHERWI THE- FO-LLO-WING- -TISS-UES - WE-PE-UNRIEMARKABLE AT MICROSCOPIC BRAIN (OR). COAGULATING GLD ICGI. LF. TESTIS EXAMINATION: - - - - - -- - - - - - - - -------------------- ---------------- ------------------(-TE-O-)-.--L-U-NG--- (-L-U-)-.--P-R-OS-T-AT-E---I-P-R-I-,-S-E-MI-N-A-L --V-E-SI-C-L-E---(-SV-I-,-U-R-ET-H-RA---- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7514 SEX: MALE DOSE GROUP: 3 DATE OF DEATH: 02412491 DATE AND TIME OF EC OPSY: STUDY DAY OF DEATH: 55 STUDY SACITIFICE STATUS: WEEK OF DEATH: I SCHEDULED SECOND INTERIM S POST-FIX WEIGHER: 12/91 9:46 NOT AVAI LA0O2(E PROSECTON: OUT" SCHNITZ TERMINAL 6()DY WEIGHT: 497 RECORDER: DENNIS NOFFMA ----------------------------------------P-A-T-HO-L-O-GI-S-T-: --D-R-.--T-O-M--PA-L-M-ER-----------------WE-I-GH-E-R-: ---PA-T-HO-L-O-GY---D-E-PA---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO 0Ra STA BRAIN JLOIRJ 2.0062 -------- ------ LIVER 24.1724 .4036 % 1.0000 WEIGHT LUNG ILU) EF. TESTIS EO11 1.8070 2.03S2 4.8627 % .3635 % 12.09489 . 007 WEIGHT AT. TESTIS 'ITE 1.9161 .4094 % 1.0145 WEIGHT WEIGHT ACCESS/SEX 040 ISOI 3.S3lO .3$SS % ------------------------------------------------------------.-7-103 % ossi WEIGHT -------------------------1-.-76-0-0------------W-E-I-GH-T--------- ORGAN NAME -------------- KEYWORDS. ;E; & P A T " 0 L 0 a y ERITY. DISTRIBUTION 0 8 S IEItV A T 1 0 N S *a & GENERAL COMMENT JOCI . .. .: G-R-O-SS---FR-E-E--T-E-X-T --C-O-M-ME-N-T-S---/---"I-S-T-O-PA-T-"-OL-O-G-IC----F-IN------- LIVER ILI) 0 OS -ALL TISSUES/ORGANS NORMAL . .. .. .. . . ... .. . .. .. .. .. . .. . LUNG (LU) . . . . . -.HY.PER.TR.OP".Y . . ".EPA.TO.CE.LLU.LA. . R: CHARACTEltiZiD'BY' A" CENTRILOSULATED PATTERN IN WHICH THE NEPATOCYIES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLUMP' APPEARAN .. ... . .. .. .. .. .. .. . .. .. .. GROSS: THIS "ENLARGED- APPEARANCE. THE CELLS ARE OTHERW MICRO: -TISSUE MISSING NECITOPSY NOTE:* )*TilkNiN6 60t@MiNi:.L6Nd @E@TiOi AliSiNd iRbm'WtT' Of TRIMMING THE FBORLALIONWIN(GB TISSUES WERE UNREMARKABLE AT MICROSCOPIC E;AHINATION: - - - - - - - - - - - - - - Ri. COAGULATING GL() (COP, LF. TESTIS (TEO), ----------------------------------------------------R-O-S-T-AT-E---(-PR-I-.--S-EM-I-N-A-L--V-ES-I-C-L-E--(-S-VI-.--U-R-ET-H-R-A--(-U-i-tl--------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABONATOITIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67585 DATE Of DEATH: 02/14/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA STUDY DAY OF DEATH: 57 DATE AND TIME OF NECROPSY: 02(14/91 8:13 PROSECT07 STUDY WEEK OF DEATH: 9 TERMINAL 6ODY WEIGHT: 450. POST-FIX WEIGHER: NOT AVAILAS PATHOLOO S:T:KEVDIR.N BILLIHOS TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-IG-H-E-R:---V-A-L--QU-I-N-N--------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0R a ---------------------BRAIN (OR) -------- -------- WEIOHT RATIO -------- S TA T ------- LIVER ILI) 2.0252 19.3391 .4493 % 1.0000 WEIGHT LUNG ILU) LF. TESTIS ITEO,ISO) NT. TESTIS TE 1.5009 I.SS53 1.5890 4.2900 % .3343 % .3451 % O.S492 .7441 .7680 WEIGHT WEIGHT WEIOHT ACCESS/SEX 0 3.0661 .3526 % .8803 % .7846 WEIGHT -------------------------------------------------------------------------------I-.S-I-4-0------------W-E-IO-H-T ----------------- OROAN NAME P A T H 0 L 0 0 Y' 0 0 S E N V A T 1 0 N S A A a KEYWORDS. SAE@E*RITY. DISTRIBUTION ----------------------------------------------------------O-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIN GENERAL COMMENT c ------------------------------------------------ LIVER ILI) Gloost: -ALL iliS6Ei/60tdAiS'N6RkA@ - .. .. .. .. .. .. .. . .. .. . .. .. MICRO: -MINIMAL .. .. .. .. .. . .. . .. .. .. .. -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED BY A CENTRILOBULA TED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A HONE 'PLUMP" APPEARAN THIS 'ENLARGED* APPEARANCE, THE CELLS ARE OTHERW THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNN-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - -------B-R-A-IN---(-OR-)-.--CO-A-G-U-L-AT-I-N-G--O-L-D--I-CG-I-.--L-F.--T-E-S-T-IS---IT-E-O-)-. --LU-N-G--(-L-U-I.--P-R-O-SwT--A--T--E----(--P--R-I--.--S-E-MI-N-A-L--V-E-SI-C-L-E--(-S-V-I.--U-R-E-T-HR-A INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7580 DATE OF DEATH: SEX: MALE DOSE GROUP: 3 STUDY DAY OF DEATH: 56 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE AND TIME OF0'4!C'R'90'PSY: 13/91 8:3s STUDY WEEK OF DEATH: I PROSECTOR: JULIE A. LARSEN TERMINAL 6ODY WEIGHT: 413. POST-FIX WEIGHER: NOT AVAI LA020/LE PATHOLOGIST: DR. TOM PAL14ER RECORDER: KEVIN BILLINGS WEIGHER: JILL BEVAN ----------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIONT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN To BRAIN 0 Ra ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S--T--A--T- BRAIN LIVER 8 ILRIJ 2.0816 -S032 % 1.0000 WEIGHT LUNG LU11 (TEIO 15.8206 11 .464se 3.8242 % 7.6002 WEIGHT LF. 7E T .600 ltl- IE TIS ITE .3541 % .3069 % .7037 .7619 WEIGHT WEIGHT ACCESS SEX ONG ISO) 1 5751 3.SO40 .3109 % $Sol % .7570 WEIGHT ---------------------------------------------------------------------------------------1-.-72-6-6------------W-E-IG-H-T--------- ORGAN NAME KEYWORDS. ;EVERITY. P DAISTTRHIBU0TILON0 a Y 0 0 S E R V A T 1 0 N S ------------------------------------------------------------- GROSS FREE-TEXT COMMENT; 4/* HISTOPATHOLOGIC FIN ------------------------------------------------ GENERAL COMMENT (Gc LIVER fLI) GROSI: -ALL iliS6Ei/6RdAiS*N6RitA@ .. .. .. . .. .. . .. . .. .. .. .. . .... .. .. . .. .. .. .. .. . .. . .. .. . .. .. .. .. .. . . .. .. .. .. . MICRO: -MINIMAL -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED BY A CENTRILOBULATED PATTERN lt4 WHICH THE IIEPATOCYTES A MORE NOMOOENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE -PLUMP- APPEARAN THIS 'ENLARGED- APPEARANCE. THE CELLS ARE OTHERW THE FOLLOWING TISSUES WERE -UNREMARKAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - --------BRAIN IBRI-.--C-O-AG-U-LA-T-I-NG----G-L-D -- I-C-G-I-.--L-F-.--T-E-S-TI-S--- (-T-E-O-)-,--L-U-NG--- I-L-U-I-.-P-R-O-ST-A-TE---(-P-R-I-.-S-E-M-IN-A-L--V-E-S-IC-L-E---(-SV-I-.-U-R-ET-H-RA--- ib INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7587 SEX: MALE DOSE GROUP: 3 DATE OF DEATH: 02412 91 STUDY DAY OF DEATH: 55 DATE AND TIME OF EC@OPSY: 02 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA STUDY WEEK OF DEATH: I TERMINAL 600Y WEIGHT: 12/91 1:59 POST-FIX WEIGHER: NOT AVAILAO(E PROSECTOR I RUTH SCHNITZ PATHOLOO S:T: Da . TOM PALMER 493. RECORDER: DENNIS HOFFMAN --------------------------------------------------------------------------------W-E-I-GH-E-R:---P-A-T-HO-L-OG-Y---D-EP-A-R---------------- OROAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Na ----------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- STA T BRAIN JONI LIVER ILIJ 2.2704 19.5473 .4605 % 1.0000 ------WEIOHT LUNG (LUI LF. TESTIS OITEO 1.5032 3.9450 % .3171 % 8.0090 .698S WEIGHT NT. TESTIS IISOI TE 1.0964 1.7142 .3441 % .7472 WEIGHT WEIGHT ACCESS/SEX a 3.904S .3477 % .8102 % .75so WEIGHT -------------------------------------------------------------------------------1.-7-5-94-------------W-EI-G-HT------------------ ORGAN NAME KEYWORDS. ; S E I V A T 10 N S --------------------------------E-V-ER-I-T-Y.---D-IS-T-R-IB-U-T-I-ON----------G-R-OS-S FREE-TEXT COMMENTS HISTOPATHOLOGIC FIN GENERAL COM14ENT ------------------------------------------------ LIVER ILIP (IAGOCSI:-ALC iliS6E@/6R6AiS*N6ltt4AE .. . ... .. . . . .... .. . .. . MICRO: -MINIMAL . .. .. .. ... .. .. .. . ... . .. .. .. .. . .. .. -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED BY A CENTRILODULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER WOUNDED GIVING THE CELLS A MORE *PLLN4P' APPEARAN THIS 'ENLARGED" APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLOW-ING-, BRAIN -TISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - - ----------------.-C-O-A-G-U-LA-T-I-N-G--O-LD---(C-O-)-.--LF-.--T-E-S-TI-S--(-T-E-O-).--L-U-N-G--(-L-U)-.--P-N-O-ST-A-T-E--(-P-R-I.--S-E-M-IN-A-L--V-E-SI-C-L-E--I-S-V-I-, -U-R-E-T-HR-A----------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7581 DATE OF DEATH: 05/17/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST POST-RECO@ DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: 571. POST-FIX WEIGHER: NOT AVAILA0O5(E17/91 7:31 PROSECTON: DEBBIE PIRKEL PATHOLOGIST: DR. TOM PALMER IIECORDER: JOE N. KIZZIEE --------------------------------------------------------------------------------W-E-I-GH-E-R:---J-A-C-K --S-ER-F-OR-T------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRANSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0na ---------------------BRAIN (BRI -------2.1057 -------.2123 % WEIGHT RATIO -------1.0000 S IA T ------WEIGHT LIVER (LIP LUNG ILU) LF. TESTIS 17.7967 1 5797 3.1129 % .2763 % 8.1423 .7227 WEIGHT WEIGHT AT. TESTIS ITTEE101 l.so4i 1.5663 .2632 % .6884 WEIGHT ACCESS/SEX 0 0 SO) 3.S672 .2740 % .6240 % .7166 WEIGHT -------------------------------------------------------------------------------1-.6-3-2-1------------W-E-IG-H-T ----------------- ORGAN NAME P A I H 0 L 0 0 V 0 0 S E ItV A T 1 0 N S a * b KEYWORDS. *SEV*ER4ITY. DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMNENTS / HISTOPATHOLO()IC FIN GENERAL COMMENT ------------------------------------------------ 'Di'OCSI-:ALL iltS6Ei/6R6AiS'N61tkA@ . . . . ... . . . . . . - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - UBNREATIN JOR(IU*ICOAGULATING OLD (COP. LF. TESTIS (TEO), LIVER (LI). LUNG (LU). PROSTATE (PRI. SE14INAL VESICLE IS -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- AHtt4AL 14UH13EN: CSISIO SEX: MALE DOSE GROUP: 3 DAIE OF DEATH: DATE AND TIME OF0541E6C/R9O1PSY: STUDY DAY OF DEATH: 141 6/91 7:47 PROSECTOR SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: 630. POST-FIX WEIGHER: NOT AVAILAOSS(EL I OAIL M. NOVAK PATHOLOO S: T : DR. TOM PALMER RECORDER: JOE IZZIEE -----------------------------------------------------------------------------WE-I-GH-E-R:----JA-C-K--"-S-il-t-F'-O-R-T ---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra -------------------- -------- TO BODY WEIGHT (Z) -------- WEIGHT RATIO -------- S TA T BRAIN I LIVER (OLR il 2.1170 .3487 % 1.0000 ------WEIGHT LUNG ILU) 21.79SI 2.1373 3.4SS2 % 9.9660 WEIGHT LF. TESTIS TEO11 2.0235 .3318 % .3208 % .9773 WEIGHT RT. TESTIS ITE ACCESS/SEX ORG (SO) 2.1199 3.61as .3361 % .9252 .9693 WEIGHT WEIGHT ------------------------------------------------------------.5-7-3-3--X --------------l-.6-s-3-6------------W-E-IG-H-T ----------------- ORGAN NAME KEYWORDS. *SEV*ERaITY. P ADISTTRHIB0UTILON0 0 Y' 0 8 S E N V A I 1 0 N S a a a -------------------------------------------------------G-R-OS-S FITEE-TEXT COMMENTS / HISTOPATHOLOGIC FIN GENERAL COMMENT c ------------------------------------------------ LIVER ILI) Glaost: -AL@ iliS6Ei/61tdAiS'N6ftP4A@ . . . . . .. .. . .. . . M.IC.RO:. .-M.OD.ERA.TE.. . F.OC.AL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GENERAL INFORMATION (XXI . . . . . . . . . . . -FATTY CHANGE GROSS: NiCiO@Si i4OiE:* )*AhlkA@ ORiOi iO*EiSAN6UiNATiOi T14-E FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN IBRI, COAGULATING GLD (CGI. LF. TESTIS (TEOI, LUNG ILU). PROSTATE (PR). SEMINAL.VESICLE (SVI, URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67SBO SEX: MALE DOSE GROUP: 3 DATE OF DEATH: 01/24/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 36 (24/91 8:58 SACRIFICE STATUS: SC14EDULED FIRST INTERIM SA STUDY WEEK Of DEATH: 6 TERMINAL 6ODY WEIGHT: 391 POST-FIX WEIGHER: NOT AVAILAoSt E PROSECTOR: JULIE A. LARSEN PATHOLOGIST: DR. TOM PALMER RECORDER: VAL QUINN ---------------------------------------------------------------------------------WE-I-G-HE-R-:--D-E-B-BI-E---PI-N-N-EL----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE OROAN TO BRAIN 0 Ra -------------------- -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- S TA BRAIN ILBINJ LIVER 2.2534 .5757 % 1.0000 -----WEIGHT LUNG (LU) LF. TESTIS TE 17.2091 1.4160 I.SS2$ 4.3968 % .3790 % .3967 % 7.6369 .6597 WEIGHT WEIGHT RT. TESTIS El0 ACCESS/SEX 0'10 ISO) 1.5279 2.S767 .3904 X .6861 .6710 WEIGHT WEIGHT ------------------------------------------------------------O-S-1-3 --% --------------1-.1-4-3-5------------W-E-IG-H-T---------------- ORGAN NAME P A T H 0 L 0 0 Y 0 0 S E R V A T 10 N S A 6 A KEYWORDS. ASEV*ER*ITY. DISTRIBUTION G-E-NE-R-AL---C-O-MM-E-NT-----------------------------------------------G--R--O-S-S---F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-H-OL-O-G-IC----F-T ------ 0100CSI:-iL@ iliS6Ei/6RdAhS*N6R'NAL* . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . - - - - - - -- -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BURREATIHNRI BR(IU*)COAGULATING OLD IC(l).LF. TESTIS (TEO). LIVER (LI), LUNG (LU), PROSTATE (PRI. SEMINAL VESICLE IS -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLDGY DATA HAZLETON MADISON. LABORATORIES AMERICA, WISCONSIN U.S.A. INC. ------------------------------------------------------------------------------------------------------------------- ADANIMAL NUMBER: COTS I SEX: MALE DOSE GROUP: 3 TE OF DEATH: 01/2!/oi STUDY DAY OF DEATH: 35 DATE AND TIME OF NECROPSY: 01/23/91 1:49 SACRIFICE STATUS: STUDY WEEK OF DEATH: 5 SCHEDULED FIRST INTERIM SA TEITMINAL 600Y WEIGHT: 379 POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: JULIE A. LARSEN PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN ---------------------------------------------------------------------------------------W-E-I-GH-E-R-:---G-A-IL---N.--N-O-V-A-K -------- ORGAN NAME -------------------- BRAIN (BRI LIVER ILI) LUNG (LU) LF. TESTIS RT. TESTIS ACCESS/SCX TEO1 0 TE la ISO) ABSOLUTE ORGAN WEIGHT (GRAMS) -------- 1.9910 10.66so 1.4326 1.5712 1.545S 2.1492 ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0R0 S TA ------ S268 % 1.0000 WEIGHT 4.3939 % 1.3413 WEIGHT .3777 % .7170 WEIGHT .4161 .7899 WEIGHT .40?S 773S WEIGHT 7512 % 1.4260 WEIGHT ----------------------------------------------- ORGAN NAME P A I N 0 L 0 0 r 0 0 S E R V A T 1 0 N S Ok a a ---------------------- K-E-Y-WO-R-D-S,----S-EV-E-R-IT-Y-,---D-I-ST-R-I-B-UT-I-O-N -----------GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl LIVER ILI) ----------------------------------------------. . . . . . . . . . . . . . . . . . . . . . ... . . . . . . . . . . . . MICRO: -MINIMAL -HYPENTROP"Y HEPATOCEEL6LAR:' 'CH'Aft'AC*TE*R'IZE*D*B*Y CENTRILODULATED PATTERN IN WHICH THE HEPATOCYTE A "ORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDE SKIN (SKI ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARA .. ... .. .. .. . . . . . . . . . . . . . . .THIS. 'ENLARGED" APPEARANCE. . THE CELLS ARE OTHER (]ROSS: -CRUSTED AREA(SI -MID DORSAL THORACIC RiGiOi:6AiK*RiD'CiUiTiD'AiE MICRO' -MODERATE. FOCAL IS X 10 MM ICAP 61 -DERMATITIS HISTOPATHOLOGICAL COMMENT: )FOCAL SUBACUTE TO CHRONIC DERNATITIS WITH EPID - --- - AND F6cAL EXUDATE FORMATION. FOCAL GRANULOt4ATO - - - - - - - - - - - - - - - - IN- TH-E D-ERMI-S -AND- AR-OUND- H-AIR- FO-LLIC-LES-. - - - APPENDIX 0 INDIVIDUAL ANIMAL PATMOLOOY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7591 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SAC DATE OF DEATH: 01423/91 DATE AND TIME OF STUDY DAY OF DEATH: 35 STUDY WEEK OF DEATH: 5 ECROPSY: 01 23/91 1:49 PROSECTOR: JULIE A. LARSEN POST-FIX WEIGHER: NOT AVAILAO(E TERMINAL BODY WEIGHT: 379. RECORDER: JILL BEVAN ------------------------------------------P-AT-H-OL-0-1-31-S-T-: --O-N-. --T-OM--P-A-LM-E-R----------------W-E-IG-H-E-R-: --G-A-IL--M-.--N-O-VA-K------------------ THE FOLLOWINGTISSUESWERE UNREMARKABLEAT MICROSCOPICEXAMINATION: BRAIN JOR).COAGULATINGOLD (CO).LF. TESTISITEOP.LUNG ILU).PROSTATE(PR),SEMINALVESICLE(SVI.URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN -U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C57SO2 DATE OF DEATH: 01/24/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHED LED FIRST INTERIM SACR DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 36 (24/91 7:47 STUDY WEEK OF DEATH: 6 TERMINANL 6ODY WEIGHT: 341.8 POST-FIX WEIGHER: NOT AVATLAoSl E PIIOSECTOlt: JULIE A. LARSEN PATHOLOGIST: DR. TOM PAL14EN RECORDER: VAL QUINN --------------------------------------------------------------------------------W-E-IG-H-E-R:---D-E-BB-I-E--P-I-R-KE-L------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R0 A ----------------------BRAIN (BPI -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- S IA I -------- LIVER ILI) 2.1769 14.0101 .4369 % 4.0989 % 1.0000 6.4351 I WEIGHT LUNG ILUI LF. TE TI OITEOJISOI 1.2998 1.5136 .3803 % S97l WEIGHT 7 WEIGHT T RT. TESTIS If ACCESS/SEX 0 1.53s2 .4428 % .4492 % SOS3 .7052 WEIGHT T WEIGHT 7 -------------------------------------2-.S-S-3-1-------------------.-77-62 % 1.2108 WEIGHT T KEYWORDS. ------------------------------------------------------ ORGAN NAME SEVERITY. DISTRIBUTION V A I10 N S G-E-NE-R-AL---C-O-MM-E-NT------c-----------------------------------------G--R--OS--S---FR-E-E--T-E-XT---C-O-MM-E-NT-S-------HI-S-T-OP-A-T-HO-L-O-GI-C----FI-N-D------- 01(olst: -ALi itiS6E@/6lt6A@S*N6Ri4Ai .. .. . .. .. THE- FO-LLO-WING- -TISS-UES- WE-RE -UNN-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON-- - - - - - - - - - - - - - BURREATIHNR IBR(IU.R)COAGULATING GLO (CG). LF. TESTIS ITEO). LIVER (LI). LUNG (LU). PROSTATE (PA). SEMINAL VESICLE ISV --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANT14AL NUMBER: C67593 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED. FIRST POST-RECO DATE OF DEATH: OS4 91 STUDY DAY OF DEATH: 141 DATE AND TIME OF 1E6C4OPSY: OS(IS/91 7:43 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: 709 POST-FIX WEIGHER: NOT AVAILAS E PROSECTOlt: JULIE A. LARSEN PATHOLOOIST: OR. TOM PALMER RECORDER: JOE N. KIZZIEE ---------------------------------------------7 -----------------------------------------W-E-I-GH-E-R-: --J-A-C-K--SE-R-F-OR-T----------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) OROAN WEIGHT RELATIVE ORGAN TO BRAIN 0 n0 ---------------------- -------- TO BOD-Y--W-EI-G-H-T - WEIO"T RATIO -------- S TA T ------- BRAIN 1001 LIVER (LI) 2.OS94 .2903 % 1.0000 WEIGHT LUNG ILU) 20.S211 2.8923 % 9.1646 WEIGHT LF. TESTIS (TEO) l.saoo 1.1691 .2199 % .2634 % . sis WEIGHT NT. TESTIS (TEI) ACCESS/SEX ORG ISO) 1.0414 3.7032 .260S % .9076 .897S WEIGHT WEIGHT ------------------------------------------------------------.-52-1-9--% --------------1-.7-9-8-2------------W-E-IG-H-T----------------- ORGAN NAME P A T " 0 L0 0 Y 0 8 S E N V A T 1 0 N S KEYWORDS. ;E*VE0RITY. DISTRIBUTION -------------------------------------------------------- OROSS FREE-TEXT COMMENT; 4/0 "ISTOPATHOLOGIC Ft OENERAL COMMENT 77 --- ------------------------------------------------ GAOOCSI: -AL@ iliS6Ei/6Ft6AhS'N6R@A@ THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - OURRAETIHNRIOTU 1,,COAGULATING GLD ICO). LF. TESTIS (TEOI. LIVER (LI). LUNG ILUI. PROSTATE (PRI, SEMINAL VESICLE (S -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- A141MAL NUMBER: C6752S SEX: MALE DOSE GROUP: 3 DATE OF DEATH: 01/23/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 3S 01 23/91 SACRIFICE STATUS: SCHEDULED. FIRST I TERIN SA STUDY WEEK OF DEATH: 5 TERMINAL BODY WEIGN HT: 414 POST-FIX WEIGHER: FOOT AVAILAO(E 8:04 PNOSECTOR: DENNIS HOFFMAN RECONDEN: JILL BEVAN ---------------------------------------------P-A-T-HO-L-O-GI-S-T-:----DN-.---TO-M--P-A-L-ME-R------------------W-E-IG-H-E-R:----GA-I-L--M-.--N-O-V-AK--------- ORGAN NAME ABSOLUTE OROAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO ONAIN 0 na ----------------------- -------- TO BODY WIEIGHT (%I -------- WEIGHT RATIO -------- STA BRAIN LIVER ILBRli 2,0247 19.3969 .4880 % 4.6?Sl % 1.0000 o.slot ------ WEIGHT WEIGHT LUNG LLIII LF. T TIS 1.5512 l.essi .3756 % @4471 % .7696 WEIGHT NT TEST TE 1.8029 .4345 % .9162 .190S WEIGHT -------A-CC-E-SS-/-S-EX----0-10-T-E-O-ls-o-l----------------3-.5-5-9-9---------------------.-$S-a-o--%---------------l-.-i-s-$-2-------------WW-EE-IIGG-HH-TT-------- ORGAN NAME ------------ --------GENERAL COMMENT P A I H 0 L 0 0 Y, 0 0 S E R V A I 1 0 N S a 6 1, KEYWORDS, *SEV-ERI-TY, DISTRIBUTION ---------------------------------------OROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl ----------------------------------------------- LIVER ILI) 0100CSI: -ALL iliS61E@/6R6AkS'N6RiiAE . M.IC.RO.: .:M.IN.IM.AL., .FO.CAL. . . . . . . . . . . . . . -.IN.FIL.TR.ATE.S..MON.ON.UCL.EA.R . . .CELL . . . . . . . . . MINIMAL -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED BY CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDE ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARA THIS "ENLARGED" APPEARANCE. THE CELLS ARE OTHER THE- FO-LLOW-INO- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN (OR). COAGULATING OLD ICUI. LF. TESTIS ITEOI. LUNG ILUI, PROSTATE (PRI. SEMINAL VESICLE ISVI, URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER- C67S94 SEX: MALE DOSE GROUP: 2 DATE OF DEATH: 02/13/91 STUDY DAY OF DEATH: 56 DATE AND 71ME'OF NECROPSY: 02 13/91 8:23 SACRIFICE STATUS: SC EDULED SECOND INTTERIM S STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGH S23 POST-FIX WEIGHER: NOT AVAILA13(E PROSECTON: DENCN)IlSt HOFFMAN PATHOLOGIST: . TOM PALMER RECORDER: KEVIN BILLING -------------------------------------------------------------------------W-E-I-GH-E-R-:---JI-L-L--B-E-VA-N------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0p a -----------------13RAIN ION) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA ------- LIVER (LI) 2.0620 21.29SS .3037 % 1.0000 WEIGHT LUNG .(LU) LF. TESTIS TEO1 1.6331 4.0056 % .3118 % 10.3276 .7020 WEIGHT RT. TESTIS TE I.IISS 1.7988 .3409 x .8659 WEIGHT WEIGHT -------ACCESS/SEX 010 isol 3.9196 .3434 % .7483 % .1724 WEIGHT ----------------------------------------------------------------------1-.-9-0-09-------------WE-I-O-HT---------------- OR-OA-N---NA-M-E-------------I-(EYWOMDS. *SEVaER*ITY. P A T "0 L0 DISTRIBUTION 0 Y' 0 9 S EA VA T 10 N S a a4 ------------------------------------G-RO-S-S FREE-TEXT COMMENTS / "ISTOPAT"OLOGIC FIN GENERAL COMMENT JOCI . . . . . . . . . . . . . . . . .I ------------------------------------------------ 0 OS : -ALL TISSUES/OR ANS NORMAL .... . . . . . . . . . . . . . . . .I . . . . . . . . . LIVER (LI) .. .. . .. .. .. .. . .. .. . HYPERTROPHY "EPATOCELLULAR: CHANA6TiRiZiD'BY' A' CENTRILOBULATED PATTE N IN WHICH THE "EPATOCYTES A MORE HOMOGENEOUS CYT4OPLASM AND THE CELL BORDER MOUNDED GIVING THE CELLS A MDRE 'PLTIMP' APPEARAN THIS *ENLARGED- APPEARANCE. THE CELLS ARE OTHEAW THE FOLLOWING T-IS-SUES-WERE-UNRIEMARKABLE BRAIN I AT MICROSCOPIC- E-XAM-INAT-ION-: - - - -- - - - -- - - - - - - - -------- JIM). COAGULATING OLD (CGI. LF. TESTIS (TEO). LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUM13IR: C67596 DATE Of DEATH: 03426/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TERMINAL SACRIFI DATE AND TIME OF ECROPSY: 03/26/91 7:41 PROSECTOR: STEVE VAN ADESTIME TERMINAL 6ODY WEIGHT: 511 RECORDER: JILL BEVAN P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-OT--A-V-AI-L-A-BL-E-----------------P-AT-H-O-LO-G-I-ST-:---D-R-. --T-ON--P-A-LM-E-R----------------W-E-I-GH-E-R-: --J-O-E--H.--K-I-Z-Z-IE-E---------------- ORGAN NA14E ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT IX) ORGAN TO BRAIN WEIGHT RATIO 0 Ra -------------------- -------- -------- -------- S TA ------ BRAIN 1L071 LIVER 2.2403 20.2379 .4380 % 1.0000 WEIGHT LUNG ILU) LF. TESTIS 1.4079 3.9566 % .2752 % 9.0336 .6284 WEIGHT WEIGHT I OITEOISOI RT. TEST S TEI 1.6654 1.6369 .3256 % .3200 % .7434 .7307 WEIGHT ACCESS/SEX 0 4.5678 .1920 % 2.0389 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME KEYWORDS, 6SEV0ERAITY. P ADISTTRHIB0UTILON0 0 Y- 0 8 S IER V A T 1 0 N S ---------------------------------------------------------G--R--O-S--S --FR-E-E--T-E-X-T--C-O-M-ME-N-T-;--0-/-*--HI-S-T-OP-A-T-H-OL-O-G-IC----F-it------- GENERAL COMMENT c LIVER (Lil G@(olsi:-ALi iftS6Ei/6RdAiS'N6Rk4 ... .. . ... .. . .. .. .. . .. .. . .. .. . .. .. .. .. . .. .. . .. . ... . MICRO: -MINIMAL, FOCAL -INFILTRATES,MONOMUCLEAR CELL -MINIMAL -HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED BY A CENTRILOBULATIED PAITERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER PROSTATE (PRI ROUI#DED GIVING THE CELLS A MORE *PLUMP' APPEARAN THIS "ENLARGED' APPEARANCE, THE CELLS ARE OTHERW . .. .. .. .. .. .. . .. .. . .. .. . .. . .. .. .. .. . MICRO: -SLIGHT, FOCAL -INFLAMMATION. CHRONIC AdTiVi THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - -------B-R-A-I-N--JOR). -C-O-A-GU-L-A-T-IN-G---OL-D--I-C-O-)-. -L-F-.--T-E-ST-I-S--(-T-E-O)-,--L-U-N-G--(-LU-)-.--S-EM-I-N-A-L--V-ES-I-C-L-E--I-S-VI-.--U-R-ET-H-R-A--J-U-R)----------------------- INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. po -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COISST SEX: MALE DOSE GROUP: 3 DATE OF DEATH: 01/24/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 36 /24/91 1:20 SACRIFICE STATUS: STUDY WEEK OF DEATH: 6 SCHEDULED FIRST INTERIM SAC TERMINAL 6ODY WEIGHT: 310. PROSECTOR: DENNIS NOFF14AN POST-FIX WEIGHER: NOT AVAILABoLlE PATHOLOGIST: DR. TOM PAL14EN ECORDER: VAL QUINN --------------------------------------------------------------------------------------W-E-IG-H-E-R:----D-EB-B-I-E --P-I-N-K-EL---------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) OROAN WEIGHT RELATIVE ORGAN 70 BRAIN 0 Na -------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO STA T BRAIN LIVER 19L0li 2.0027 19.4228 .5270 % S.1113 % -------- 1.0000 9.6913 ------- WEIGHT WEIGHT LUNG ILU) LF, TESTIS TEO 1.4474 1.4949 .3800 % .3934 % .7227 WEIGHT AT. TESTIS TEI ACCESS/SEX 010 ISO) 1.5568 .4097 % .7464 .7774 WEIGHT WEIGHT ----------------------------------------2-.S-S-GO----------------------.6-9-8-9--%---------------1-.-3-2-62--------------W-E-IG-H-T--------- ORGAN NAME KEYWORDS. iw m A P A T H 0 L 0 0 V' 0 9 S E R V A T 1 0 N S SEVERITY, DISTRIBUTION haa ---------------------- GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC FINE GENERAL COMMENT JOCI ---------------------------------------------------------------------------------------- LIVER ILI) 0 OS : -ALi iliS6lEi/6R6AiS'N6RkA@ .. .. .. .. . ... .. . .. .. . .. .. .. .. .. .. .. . .. . .. .. .. .. . .. .. . .. .. . MICRO: -MINIMAL -HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED BY AN CENTRILOSULATED PATTERN IN WHICH THE fiEPATOCYtEs A MORE "OMOOENEOUS CYTOPLASM AND THE CELL BORDERS RTOHUINSDED OIVING THE CELLS A MORE 'PLUMP' APPEARAt4C - - - - - - - - - - - - - - - - -- - - - - - - - 'ENLARGED' APPEAltANCE. THE -CCLLS-ARE OTHERWI THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (OR). COAGULATING OLD ICGI, LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE ISVI. URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7596 DATE OF DEATH: 03/26/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFI STUDY DAY OF DEATH: 97 DATE AND TIME OF NECROPSY: 03 26101 1:13 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: 534 POST-FIX WEIGHER: NOT AVAILAB(C PROSECTOR: DEBBIE PINKEL PATHOLOGIST: ON. TOM PALMER RECORDER: JILL BEVAN - ----------------------------------------------------------------------W-E-IG-H-E-R:---J-O-E-N-.--K-I-Z-ZI-E-E--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0na -------------------BRAINR JLOIRJ -------- TO BODY WEIGHT -------- WEI-GH-T---R-AT-I-O S TA ------ LIVE 2.0731 22.4725 .3880 % 1.0000 WEIGHT LUNG LU) LF. TELTI 1.6013 4.2060 1 .3010 % 10.1400 .7758 WEIO"T WEIGHT AT. TESTIS TEI0I 1.6629 1.6661 .3112 % .8021 WEIGHT ACCESS/SEX 0 a (Sol 2.8754 .3118 % S3$2 % .803? WEIGHT --------------------------------------------------------------------------------------1-.-3-17-0-------------WE-I-GH-T-------- ORGAN NAME ---- ------- KEYWORDS. ;E*VERAITYP, DAISTTRHIBU0TILON0 0 Y 0 8GRSOSES 4FRVEEA-TETXT1 0CONMMSENTS a a/ a HISTOPAT"OLOOIC Fl GENERAL COMMENT ----------------------------------------------- G@GOCSI: -A-L-L -TIS'S'UE'S/'ORG'A'NS'N'ORKAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . LIVER ILI) THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE -- --AT MICROSCOPIC -"YPEIRiR6PhY. @[OA-TOC-EL-L-ULA.R:.-C"'AR'AC'TE'RIZED-BY A CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTEI A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLtN4P' APPEARAP THIS -ENLARGED- APPEARANCE. THE CELLS ARE OTHER@ --- -- -- --- --- --- ---EXAMINATION: -------B-RAIN IOR). COAGULATING (ILD ICO). LF, TESTIS (TEO), LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SV). URETHRA ---------------------------------------------------------------------------------------------------------- L4 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67S99 DATE OF DEATH: 01/23/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 35 01 23/91 9:17 PROSECTOR: STUDY WEEK OF DEATH: DENNIS HOFFMAN 5 TERMI NAL 1 ODY WEIGHT: 407 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-IG-H-E-R-: --G-A-I-L --M.--N-O-VA-K----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra BRAIN LIJ TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA ------ LIVER 1.9288 20.7104 .4733 % 1.0000 WEIGHT LUNG ILU) LF. TESTIS TEO1 1.5289 5.0843 Ix .3752 % 10.7416 .7927 WEIGHT WEIGHT RT. TESTIS TE 1.7114 1.6042 .4200 % .8173 WEIGHT ------A-C-CE-S-S/-S-E-X ---0-1' -0--I-S-O-)--------------2--.-3--1--4--S-- .4133 % 5152 % .8732 1.2303 WEIGHT WEIGHT --------------------------------------------------------------------- OR.3AN NAME -------------- GENERAL COMMENT LIVER ILI) act GIOS KEYWORDS. P A I " 0 L 0 0 Y 0 8 S E R V A T 10 N S a - - ;EVERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FIN . . . . . -.-.- .--.-.--.--.-.--.--.--.-.--.--.--.-.--.--.-.--.--.--.-.--.--.--.-.------- -ALL TISSUES/ORGANS NORMAL . .. .. .. . .. .. .. . .. .. .. .. .. . . ... .. .. HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED By ii CENTRILOBULATED PATTE " IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTaOPLASM AND THE CELL BORDER GENERAL INFORMATION ixxi GROSS: ... . . ... . .. ROUNDED GIVING THE CELLS A MDRE 'PLUMP' APPEARAN THIS *ENLARGED" APPEARANCE, THE CELLS ARE OTHERW HiCiOOSi iOiE:' )'AiliqAi61ii).Pil6R.T6 iXiA*N'GU*IN*AT'I'ON* THE- FO-LLOW-IN 8-0R, -TISS-UES- WE-RE BRAIN ( -UNR-EMA-RMA1-3LE- AT- MI-CRO-SCOP-IC - EX-AN I NA T10 N: - - - - - - - - - - - - - - - -------- . COAGULATING GLD (C(li. LF. TESTIS ITEO). LUNG (LU). PROSTATE IPR). SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C67000 DATE OF DEATH: 03/26/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIF STUDY DAY OF DEATH: 97 STUDY WEEK Of DEATH: 14 DATE AND TIME OF NECROPSY: 03/26/91 9:26 PROSECTOR: DEBBIE PIRKEL TE tMINAL 6ODY WE IGHT: SO POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-I-O-HE-R-:--J-O-E--H-.--KI-Z-Z-I-E --------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R (GRAMS) TO BODY WEIGHT ---------------------BRAIN -------- -------- WEIGHT RATIO -------- S TA ----- LIVER ILBRII 2.257S 24.7003 .3793 % 4.ISOB % 1.0000 WEIGH LUNG (LU) LF. 'TESTIS ITEO) 1.7639 1.7801 .2904 % 10.9414 .7114 WEIGH WEIGH NT. TESTIS O'ITEIISO) ACCESS/SEX 1.7600 .2991 % .2957 % .7815 .7796 WEIGH WEIGH ------------------a-------------------3-.-56-9-7--------------------SO-O-$ --%--------------l-.-s-s-l-3------------WE-I-GH---------------- ORGAN NAME ItA 01 P A T N 0 L 0 0 V 0 8 S I R V A T 1 0 N S A A 0 KEYWORDS. SEVERITY. DISTRIBUTION ----------------------------------------------------------G--R--O--S-S--F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---"I-S-T-O-PA-T-"-OL-O-G-I-C ---F------ GENERAL COMMENT LIVER ILI) GI(OICSI-:AL@ iliS6Ei/6RdAiS*N61tkA@ . ... .. .. ... .. .. .. . .. .. .. .. .. . .. . .. . .. .. .. MICRO: -MINIMAL. FOCAL -INFLAMMATION,GRANUL16MAT*OU'S -Ml INAL -HYPERTROPHY NEPATOCELLULAR: CHARACTERIZED BY CENTRILODULATED PATTERN IN WHICH THE NEPATOCVTE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDE ROUNDED GIVING THE CELLS A MORE "PLUMP' APPEARA THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHER THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN IBRI. COAGULATING OLD (CO). LF. TESTIS (TEO), LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE (SVI, URETHRA ------------------------------------------------------------------------------------------------------------------- 4.n INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?Gol SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SC EDULED. FIRST INTERIM SAC DATE OF DEATH: 01/25/21 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 /2S/91 8:41 PROSECTOR:, JULIE A. LARSEN TENtMINAL BODY WEIGHT: 410. POST-FIX WEIGHER: NOT AVAILAoBlLE PATHOLOO ST: DR. TOM PAL14ER RECORDER: JILL BEVAN WEIGHER: PATHOLOGY DEPAR -------------------------------------------------------------------------------------------------------------------- OROAR NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 Ra ---------------------BRAIN IoLnIJ -------2.1563 -------.4487 % WEIGHT RATIO -------1.0000 ST A T ------WEIGHT LIVER LUNG ILU) 21.892S l.7051 4.SSS2 % .3S40 % 10.IS28 WEIGHT Lf. TESTIS ()ITE1O1 RI. TESTIS TE 1.6701 .3475 % .7908 .774S WEIGHT WEIGHT ACCESS/SEX 0 (SO) 1.7349 3.3149 .3610 X .0897 % .8046 l.s373 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 0 Y@ 0 8 5 E R V A T 1 0 N S KEYWORDS. ;E*VE0RITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T --C-O-M-ME-N-T-;--*-/-*--HI-S-T-OP-A-T-HO-L-O-GI-C----F-IN-C------- COAGULATING OLD Gical . . . . . . . . . . . . . . . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . . os . NECROPSY NOTE: )-TRIMMING COMMENT: MISSING FROM WET TISSUE At TIME MICRO: -TISSUE MISSING GENERAL COMMENT LIVER (LI) PROSTATE IPRI SEMINAL VESICLE 101'OCSI: -ALL iliS6Ei/6RdAiS'N6R'MAi . .. . .. .. . .. .. MICRO: .-illiIAA@ -HYPERTROPHY* "EPATOCELLULAR: 'C"ARACTERIZED-13Y AN CENTRILODULATED PATTERN IN WHICH THE "EPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE "PLUMP' APPEAltANC THIS -ENLARGED" APPEARANCE, THE CELLS ARE OTHERWI . .... .. . .. .. .. . .. .. .. . .. .. . .. .. .. . .. .. . ... . . ... . GROSS: MICRO: -TISSUE MISSING NECROPSY NOTE: >TRIMMING COMMENT: MISSING FROM WET TISSUE AT TIME (SVI . . . . . . . . . . . . . URETHRA JURP MICRO: -TISSUE MISSING HiCiOOSi . ... .. .. . .. .. . .. . GROSS: HiCiOOSi hOiE* )Tilt@MiN4 60AMiNi:'MiSiliG'FiOI4 @$Ei il@S6E'Ai ilite iOiE*: )TOIAMiNd 60@*4iNi:'MiSiliG*FiCO4 t4Ei iliS6E'Ai ilke cn APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- AtilMAL NUMBER: C67601 DAIE OF DEAIH: 01/25/91 SIX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACIT STUDY DAY OF DEATH: 37 DATE AND TIME OF NECROPSY: 01(25/91 8:41 STUDY WEEK OF DEATH: 6 TERMINAL 600Y WEIGHT: 480.6 POST-FIX WEIGHER: NOT AVAILAO E PROSECTOR:,,JULIE A. LARSEN PATHOLOO S : DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-EI-G-H-ER-:---P-AT-H-O-LO-G-Y---DE-P-A-RT----------------- ORGAN NAME h a 1% P A T H 0 L 01(iy 0 0 S E R V A T 1 0 N S (CONTINUED) ---------------------K--E1Y-W-RD-S-,---S-E-V-ER-I-T-Y-. --D-IS-T-R--B-U-TI-O-N---------G-R-O-SS FREE-TEXT COMMENTS / ;I;T;PATHOLOGIC FIND URETHRA JUR) . . . . ------------------------------------------------- MICRO: -iliS6E*MiSiliG' . . . . . . . . . . . . . . . ... . . . . . . TH-EF-OLL-OW-IN-TGIS-S-U-EWS-ERE-U-NR-EM-AR-KAA-BTLME-ICR-OS-CO-PEI-XCA-MIN-AT-I-O-N - - - - - - - - BRAIN IOR), LF. TESTIS ITEO). LUNG (LU) ---- -------- ----------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- ANIMAL flUMBER: CG?602 SEX: MALE DOSE GROUP: 3 DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 DATE AND TIME OF NECROPSY: 02(14/91 8:4$ SACRIFICE STATUS: SCHEDULED. SECOND INTERIM SA STUDY WEEK OF DEATH: 9 TERMINAL BODY WEIGHT: 504. POST-FIX WEIGHER: NOT AVAILAD E PROSECTON: KEVIN BILLINGS RECORDER: JILL BEVAN ------------------------------------------P-AT-H-O-LO-G-I-ST-:---D-R.---T-OM--P-A-LM-E-R----------------W-E-IG-H-E-R:---V-A-L --QU-I-N-N --------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSO ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 0a ---------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S--T--A--T- BRAIN ILFIJR LIVER LUNG (LU) 2.0978 20.2097 1.9142 .4162 % 4.0099 % 1,0000 9.0338 WEIGHT WEIGHT LF. TESTIS ItT. TESTIS TEII 1.9214 .3203 X .3812 X .7695 .9159 WEIGHT WEIGHT 0 ACCESS/SEX 0 a SO) 1.8826 3.0233 .373S % .5999 % .1974 1.4412 WEIGHT -----------------------------------------------------------------------------------------------W-E-IG-H-T----------------- ORGAN NAME PA TH0 L0 0 Y 0 0 S EN VA I 10N S &* A KEYWORDS. ;E*VERITY, DISTRIBUTION G-E-NE-R-AL---C-O-MM-E-NT---------------------------------------------------G-R-O-SS---FR-E-E---TE-X-T--C-O-H-WN-T-S----/---H-IS-T-O-PA-T-"-O-LO-G-I-C---FI-N------- LIVER (LI) 0400CSI:-iLi itiS6Ei/6R6AkS'N6RitAi . M.IC.RO.: .-M.IN.IM.AL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -HYPERTROPHY "EPATOCELLULAR: CHARACTERIZED BY At CENTRILOSULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE "PLUMP' APPEARANC THIS 'ENLARGED" APPEARANCE. THE CELLS ARE OT"ENWI THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-A141-MAT-ION:- - - - - - - - - - - - - - - BRAIN (BRI, COAGULATING OLD (CGI, LF. TESTIS (TEOI. LUNG ILU). PROSTATE IPR). SEMINAL VESICLE (SVI. URETHRA I ------------------------------------------------------------------------------------------------------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67803 DATE OF DEATH: 02122191 SEX: MALE DOSE GROUP: 3 STUDY DAY OF DEATH: 93 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFI DATE AND TIME OF NECROPSY: 03/22/91 9:38 STUDY WEEK Of DEATH: 14 TE RMINAL 6ODY WEIGHT: 609 POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: KEVIN BILLINGS PATHOLOGIST: DR. T014 PALMER RECORDER: JOE W. KIZZIE ---------------------------------------------------------------------------------------W-E-I-GH-E-R-:---J-I-LL---BE-V-A-N----------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JUNA"SL ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 na ----------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA ------ BRAINR JLORli LIVE 2.1709 .3S12 % 1.0000 WEIGHT LUNG (LU) LF. TESTIS 22.3081 1.7242 1.6144 3,6607 % .2820 X .2164 % 10.2760 .7942 .77se WEIGHT WEIGHT WEIGHT AT. TESTIS TEI ACCESS/SEX 0 0 SO) 1.6807 3.2306 .2761 X .5301 .7770 WEIGHT ----------------------------------------------------------------------%----------------1.-4-1-8-1-------------W-E-I-GH-T-------- P A T H 0 L 0 0 Y' 0 0 S f P V A T 1 0 N S b a e ORGAN NAME ---------------------- K-E-Y-WO-R-D-S,----;-E-*V-E-R-IT-Y-,--D-I-S-TR-I-B-UT-I-O-N-----------GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl GENERAL COMMENT ----------------------------------------------- LIVER ILI) GI(OICSI: -ALE il@S6Ei/61tdAiS*N6R?@AE . . . . ... ... .. .. . . ... . .. .. .. . .. .. . .. .. .. .. .. . .. .. .. .. . MICRO: -MINIMAL -"YPENTROPHY "EPATOCELLULAR: CHARACTERIZED BY 0 CENTRILOBULATED PATTERN IN WHICH T14E HEPATOCYTEI A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP* APPEARAP PROSTATE (PRI ... ... . MICRO: -SLIGHT. THIS 'ENLARGED" APPEARANCE. THE CELLS ARE OTHEft . . . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MULTI-FOCAL -INFLAMMATION. CHRONIC ACTIVE THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNRE-MAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATIO-N: - - - - - - - - ------BR-A-I-N---IB-R-I-.--C-O-AG-U-L-A-T-IN-G---O-L-D--I-C-O-)-.--L-F.---T-ES-T-I-S---(T-E-O-)-.--LUNG (LU), SEMINAL VESICLE ISV). URETHRA JUNI ---------------------------------------------- APPENDIX 0 INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67604 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF DEATH: 03/22/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 (22/91 9:10 PROSECTOR: RUTH SCHNITZ TERMINAL 600Y WEIGHT: S56. POST-FIX WEIGHER: NOT AVAILA0S3 E PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE ---------------------------------------------------------------------------------WE-I-G-HE-R-:---JI-L-L--B-EV-A-N-------------------- ORGAN NAME A13SOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ---------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA T ------- BRAIN (OR) LIVER ILI) 2.1210 .3814 % 1.0000 WEIGHT LUNG (LU) 19.0391 3.SISS % 9.4001 WEIGHT LF. TESTIS 01TE?Isol 1.7130 1.9040 .3010 x .3424 X .1076 .0977 WEIGHT RT. TESTIS TE ACCESS/SEX 0 1.8120 3.439S .3384 % .8873 WEIGHT WEIGHT ------------------------------------------------------r------------s--i-l-s--%--------------1-.-6-21-0-------------WE-I-GH-T--------- ORGAN NAME P A T H 0 L 0 0 Y 0 8 S E A V A T 10 N S KEYWORDS. ;E*VERITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S-S---F-RE-E---TE-X-T--C-O-M-ME-N-T-; --&-/-*--HI-S-T-OP-A-T-HO-L-O-OI-C----F-IN-C------- GENERAL COMMENT c LIVER (LI) Ol'oost: -iL@ iliS6Ei/6R6AhS'N61tkA@ . . .. MICRO: .. .. .. - . -SLIGHT. FOCAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -MINIMAL -INFILTRATES,MONONUCLEAR CELL -HYPENTROPHY "EPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOOENIOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANC THIS 'ENLARGED* APPEARANCE, THE CELLS ARE OTHERWI THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON.-- - - - - - - - - - - - - - - BRAIN JOR). COAGULATING OLD ICO), LF, TESTIS ITEO). LUNG ILUI. PROSTATE (PRI. SEMINAL VESICLE ISV), URETHRA I ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HMAAZDLIESTOONN. WLIASBCOORNATSOIRNIES U.SA.MAER.ICA, INC. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CGIGOS SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFI DATE OF DEATH:,03F 426/91 STUDY DAY OF DEATH: 97 DATE AND TIME ECROPSY: 03(26/91 9:39 STUDY WEEK OF DEATH: 14 TE RHI NAL 6ODY WEIGHT: S73 POST-FIX WEIGHER: NOT AVAILAO E PROSECTOR: DEBBIE PIRKEL PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN WEI(INER: JOE M. KIZZIEE ------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN WEIGHT RATIO 0 R (I ---------------------- -------- -------- -------- STA ------ BRAIN JOR) LIVER (LI) LUNG ILU) F. TESTIS 2.2442 22.2101 I.GS79 .3915 % 3.8748 % .2892 % 1.0000 9.8989 .7388 WEIGHT WEIGHT WEIGHT L RT. TESTIS TEII 1.7710 1.7167 .3091 % .3117 % .7194 .7902 WEIGHT WEIGHT ------A-C-CE-S-S/-S-E-X ---0--a--(-S-O-)--------------3.-2-6-3-4-------------------.-56-9-3--% --------------1-.4-S-4-2------------W-E-IG-H-T ---------------- ORGAN NAME P A T H 0 L 0 0 Y' 0 8 S E A V A T 1 0 N S 0 a a KEYWORDS. S*EV*ER0ITY. DISTRIBUTION GROSS FREE-TEXT CCM4ENTS / HISTOPATHOLOGIC Fl ------------------------------------------------------------------------------------------------------------ GENERAL COMMENT LIVER ILI) OA(OICSA-:ALE iliS6Ei/6RdAiS'N6Ri4A@ .I... .. .. I .. .. .. .. .. .. . .. . . .. .. . .. ... . .. .. . .. .. .. MICRO: -MINIMAL -"YPENTROPHY NEPATOCELLULAR: CHARACTERIZED BY A CENTRILOSULATE() PATTERN IN WHICH THE "EPATOCYTES A "ORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAN THIS "ENLARGED" APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN JOR). COAGULATING GLD (COI, LF. TESTIS ITEO). LUNG (LU). PROSTATE IPR). SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. t4ADISON, WISCONSIN U.S.A, ------------------------------------------------------------------------------------------------------------------ AN114AL NUMBER: CB?606 DATE OF DEATH: 02/14/91 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED SECOND INTERIM STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 0 TEITNI MAL 6ODY WEIGHT: 47 DATE AND TIME OF NECROPSY: 02/14/91 8:34 PROSECTOR: KEVIN BILLINGS RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TON PAL14ER WEIOHER: VAL QUINN ------------------------------------------------------------------------------------------------------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT III ORGAN TO BRAIN WEIGHT RATIO 0R S TA ---------------------- -------- -------- -------- ----- BRAIN (ON) LIVER ILI) 2.0932 16.7844 .4398 % 3.S2$9 % 1.0000 WEIGH LUNG LUI LF. TE Ti TEO 1.2778 .2485 % 8.0115 .610S WEIGH WEIGH 1 RT. TE TIS ITE 1.7686 1.1576 .3716 % .3903 % .8449 .8874 WEIGH ------A-C-CE-S-S--S-E-X--O-R-O---ls-o-l--------------2-.-92-1-7-------------:------------.--8-13-9--%--------------1-.-3-9-SI-------------WW-EEII-GG-HH ------- ORGAN NAME P A I " IDL 0 0 V 0 0 S E R V A T 1 0 N S 0 0 a KEYWORDS. ;EV*ER0ITY. DISTRIBUTION .. GROSS FREE-TEXT COMMENTS / HISTOPATHOLOCKIC F ------------------------------------------------------------------------------------------------------------ GENERAL COMMENT c LIVER ILI) GI(olst:-AL@ iliS6Ei/6ft6AiS'N61ti4A@ . . .. . . .. . . .. . . .. . . .. . .. . .. . . . . . .. .. . . .. . . . .. . .. .. MICRO: -MINIMAL -"YPENTROPHY ITEPATOCELLULAR: CHARACTERIZED BY CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTE A MORE HOMOGENEOUS CYTOPLAS14 AND THE CELL BORDE ROUNDED GIVING THE CELLS A MORE "PLUMP* APPEARA T"IS_*ENLARGED* APPEARANCE. THE CELLS ARE OTHER - -- - -- - - -- --- - THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON-- BRAIN (ON), COAGULATING OLD (CO). LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE ISVI. URETHRA ------------------------------------------------------------------------------------------------------------------ t@3 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7607 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRI DATE OF DEATH: 03/26/91 STUDY DAY OF DEAT"@ 97 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: S DATE AND TIME OF NECROPSY: 03 26/91 8:05 PROSECTON: STEVE VAN ADESTIME RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: 04. TOM PALMER WEIGHER: JOE M. KIZZI - ------------------------------------------------------------------------------------------------------ ORGAN NAME ---------------------- ABSOLUTE ORGAN WEIGHT (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0R ST ---- GRAIN JOR11 2.OS24 .3894 % 1.0000 WEIG LIVER L 17.1774 3.3011 % 8.7105 WEIG LUNG JLUI .9333 .1771 % .4547 WEIG LF. TE TI 1.0299 .3472 % .1910 WEIG RT. TESTIS TE 1.8216 .3450 % .087S WEIG -------ACCE-S-S/-S-E-X ---01-0-T-EO-l-s-ol---------------2-.5-3-7-4-------------------.-4-81-4--X--------------1-.-2-36-3-------------WE-I-G------------- PA TH 0 L0 0 Y 0 8 S E NV A T10 N S A& A ORGAN NAME KEYWORDS. S*EV*ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC -------------------------------------------------------------- --------------------------------------------- GENERAL COMMENT GI(OICSI-:iLi iliS6Ei/6lt6AiS'N6RkA@ - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN,,,IBIRI,,COAGULATING OLD (CO). LF. TESTIS ITED), LIVER ILI). LUNG ILU). PROSTATE IPR). SEMINAL VESICLE UPET u -------- ------------------------------------------------------------------------------------------------------- i.n w INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67608 SEX: HATE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF DEATH: 03/25/91 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 DATE AND TIME OF NECROPSY: 03(2S/91 1:36 PROSECTON: JACK SERFORT TERMI MAL 6ODY WEIGHT: Soo. POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST! DR. TOM PALMER RECORDER: JOE N. KIZZIEE ---------------------------------------------------------------------------------------W-E-IG-H-E-R-: ---S-TE-V-E---VA-N--A-D-E-ST-------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) OROAN TO BRAIN WEIGHT RATIO 0 R (I SIA I --------------------- -------- -------- -------- ------- BRAIN LIVER ILBNli 2.02493: 17 8 .4113 % 3.4489 1.0000 WEIGHT LUNG ILU) I.S341 % .3067 % 8.2394 .7332 WEIGHT WEIGHT LF. TESTIS .ITIEEIIISO, RT. TESTIS I.7IS2: 1. 69 .3703 % .3s37 Issl WEIGHT ACCESS/SEX 0 3.204S -------------------------------------------------------------- % .6404 % .84S4 1.5308 WEIGHT WEIGHT ----------------------------------------------------- a a a P A I H 0 L 0 0 Y 0 8 S E R V A I 1 0 N S ok a it ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION ---------------------- ----------------------------------- GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl" --- ------------------------------------------------ GENERAL COMMENT LIVER ILI) 0100CSI: -ALi iliS6Ei/bRdAAS*N61tkA@ -MINIMAL -INFILTRATES,MONONUCLEAR CELL -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED BY A CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARAN THIS_"ENLARGED' APPEARANCE, THE CELLS ARE OTHERW - --- -- --- - THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON.-- BRAIN IBRI. COAGULATING OLD ICG). LF. TESTIS ITEO). LUNG ILU), PROSTATE IPRI. SEMINAL VESICLE ISVI. URETHRA ----------------------------------------------------------------------------------------------------------------- ,-n INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA MHAADZILSEOTONN. WLIASBCOORNATSOIRNIES U.SA.MAER.ICA. INC. Pol --------------------------------------------------------------------------------------------------------------------- ANIMALNUMBER:C67609 SEX: MALE DOSE CIROUP: 3 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE Or DEATH: OS/17/91 STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL AODY WEIGHT: 730.9 DATE AND TIME OF NECROPSY: 8:53 PROSECTOR: JULIE A. LARSEN RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILA0S5(1E7/91 PATHOLOGIST: Oft. TOM PALMER WEIGHER: DENNIS HOFFMAN ----------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0A0 A ORGAN NAME ---------------------- (GRAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T -------- BRAIN ( LIVER (OLRil 2.2519 18.0752 3091 % 2*4730 % 1.0000 8.0018 WEIGHT 7 WEIGHT T LUNG (LUI 1.5208 .2081 % .6732 WEIGHT T LF. TESTIIS OITEO11 NT. TEST S TE 1.7ses 1.8611 .2403 % .2SSB % .7776 .8270 WEIOHT T WEIGHT ACCESS/SEX ------- ---a --lsof --------------3.-2434 ------------------.-4-438 % 1.4358 T WEIGHT I --------------------------------------------- a & a P A T H 0 L 0 0 V ' 0 0 S E 0 V A T 1 0 N S a e ft O-R-G-AN---NA-M-E-------------K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R-O--S-S---F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-H-OL-O-G-IC----F-IN-D------GENERAL COMMENT 04(OICSI-:ANiPtiL'OiEiE ... . . . . . . . . . . . . . . . . . . . . . . . . . . ---- ------ ---- --- -- --- --- -- --- --- ---- -- THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BURREATIHNR@ IBJRUIA) COAGULATING OLD (CO), LF. TESTIS (TEO). LIVER ILIJ, LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SV -------- ---------------------------------------------------------------------------------------------------------- 4-n INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABONA70RIES AMERICA. INC. 14ADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?610 SEX: MALE DOSE GROUP: 3 SACITIFICE STATUS: SC"ED LED TERMINAL SACRIFICE DATE OF DEATH: 03/25/91 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 DATE AND TIME OF NECROPSY: 03 25/91 0:58 PROSECTON: JULIE A. LARSEN TERNINUAL iODY WEIGHT: S41.1 POST-FIX WEIGHER: NOT AVAILAB(E PATHOLOGIST: DR. TOM PALMER ITECONDER: JOE M. KIZZIEE WEIGHER: STEVE VAN ADESTI --------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRANSI ORGAN WEIGHT ITELATIVE ORGAN TO BRAIN 0* 0A ---------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- STA I -------- BRAIN JONI LIVER 2.4094 18.4S$3 .4396 % 3.3677 % 1.0000 WEIGHT I LUNG (LU) LF. TESTIS ITEO) 1.7268 .31SI % 7.6010 .7167 WEIGHT TI WEIGHT OT. TESTIS TEII 1.73SO 1.7303 .319S % .3172 % .7201 .721S WEIGHT TA WEIGHT ------A-C-CE-S-S-/S-E-X---01-0----S-ol---------------4.-0-0-26--------------------7-3-0-3 -%----- 1.6612 TA WEIGHT TA -------------------------------------------------- ORGAN NAME P A I " 0 L 0 0 Y' 0 8 S E ItV A T 1 0 N S a % A KEYWORDS, SEVEITITY. DISTRIBUTION -------------- GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FINDI GENERAL COMMENT -------------------------------------------------- oa'oc-sALlL:iliS6Ei/61tdAiS*R6NkAC . . . . . . . . . . . . ... . . . . . . . . . LIVER ILI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -MINIMAL -HYPENTROPHY "EPATOCILLULAR: CHARACTERIZED BY AN CENTRILOSULATED PATTERN IN WHICH THE HEPATOCYTES A A MORE HOMOGENEOUS CYTOPLASM AND THE CELL DOITOERS ROUNDED GIVING THE CELLS A MORE 'PLTMP' APPEARANCE THIS *EIILARGED" APPEARANCE. THE CELLS ARE OTHERWIS THE- FO-LLO-WINGBRAIN I -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MICROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - - - OR). COAGULATING OLD ICO). LF. TESTIS (TEO). LUNG fLU). PROSTATE (PRI, SEMINAL VESICLE ISVI. UltETHItAJU --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- AN114AL NU14BERi CG?Slt SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF DEATH: 03/25/91 STUDY DAY OF DEAT14: 06 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WE1014T: 520. DATE AND TIME Of NECROPSY: 03(2S/21 9:21 PROSECTOR: JULIE A. LARSEN RECORDER: JOE H. KIZZIEE POST-FIX WEIGHER: NOT AVAILAS E PATHOLOGIST: DR. TOM PALMER WEIGHER: SIEVE VAN ADEST ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN MA14E ---------------------- (GRANSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- ORGAN TO GRAIN WEIGHT RATIO -------- 0 Ra S TA T GRAIN Bt 2.L$43 .4121 % 1.0000 WEIGHT LIVER ILII LUNG ILU 18.8888 t.4205 3.5693 % .2484 % 8.6475 .6503 WEIGHT WEIGHT LF. TE TEO 1.6407 .3100 x .7511 WEIGHT AT. TESTTI,S TEI 1.7118 .323S % .7837 WEIGHT ACCESS/SEX 0 I0 SO) 3.9s$3 .7480 % 1.1122 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 Y' 0 0 S E R V A T 1 0 N S A * A ORGAN NAME KEYWORDS, SE:ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC FIN ------------------------------------------------------------------------------------------------------------ GENERAL LIVER COMMENT 0100CS1 : -iLE iliS6Ei/6R6AiS'N6ltkAL . . . . ....* . . . . . . . ILZI . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -MINIMAL, FOCAL TiFiLiftAT*ES'."6ON6ONUCLE iR'CiLi -MINIMAL -HYPERTROP HEPATOCELLULAR: CHARACTERIZED BY A CENTRILOSUL"YATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAN THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - --- --- - BRAIN IOR). COAOULATINO GLD (CO). LF. TESTIS ITEO), LUNG (LUI. PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA ----------------------------------------------------------------------------------------------------------------- .Mb INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- Al#ll4ALNUMBER: CS7612 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE OF DEATH: 02/12/91 STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL IOOY WEIGHT: 469. DATE AND TIME Of NECROPSY: 02(12/91 S:02 PROSECTOlt: NANCY DIEDRICH RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILAO t PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY DEPAR -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 pa ORGAN NAME ----------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S--T--A--T- BRAIN ON 2.0627 .4390 % 1.0000 WEIGHT LIVER ILIJ 19 6334 4.1782 X O.Si3 WEIGHT LUNG ILUI I.S$19 .3339 % .7606 WEIGHT LF. TE t TEO 1.6686 .35SI % .1089 WEIGHT RY. TESTIS ITEI ILS95S .3601 % .4220 WEIGHT ACCESS/SEX 0 0 $01 2.9461 .6271 % 1,4216 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S a a * O-R-G-AN---NA-M-E -------------K--E-Y-WO-R-D-S.---;-E-AV-E-R-IT-Y-.--DI-S-T-RI-B-U-TI-O-N----------G--R--O--S-S--F-RE-E---TE-X-T--C-O-M-EN-T-S----/---"I-S-T-O-PA-T-H-OL-O-O-T-C ---F-tH-i------ GENERAL COMMENT (II(OICS-IA-Li iIiS6Ei/6RdAiS'N601AAi - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN IBR) COAOULATING OLD ICO). LF. TESTIS (TEO). LIVER ILI). LUNG (LU), PROSTATE IFNI. SEMINAL VESICLE IS -------O-R-E-T-HR----(U-i-l----------------------------------------------------------------------------------------- Ln co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C07613 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE Of DEATH: STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TEWMI tiAL 6ODY WEIOH7: 583. DATE AND TIME OF034EIC14O9P,SY: 03(22/91 7:24 PROSECTOR: KEVIN BILLINOS RECORDER: JOE N. KIZZIEE POST-FIX WEIOHER: NOT AVAILAB E PATHOLO(IIST: Dlt. T004 PALMER WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WIEIGHT OROAR NAME ---------------------- (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Aa STA T ------- BRAIN IBN11 2.2002 .3773 % 1.0000 WEIGHT LIVER L 21.3304 3.SS75 X 9.6941 WEIGHT LUNG ILUI 1.7482 .2998 % '7946 WEIGHT LF. TE TI OITEO ISO) 1.6482 .2120 % .7491 W[IOHT OT. TESTIS TE 1.66si .2lS5 X .7561 WEIGHT ACCESS/SEX a 3.1010 S310 % 1,4007 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 Y' 0 8 S E N V A T 1 0 N S a iba ORGAN NAME KEYWOOTOS. ;EAVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FIN ------------------------------------------------------------------------------------------------------------- GENERAL COMMENT GIGOCSI:-ALi il@S6Ei/61tdAiS*N6lti4Ai - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN ION), COAGULATING OLD ICO). LF. TESTIS (TEO). LIVER (Lil. LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE (S URETHRA (Ult) ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67014 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED SECOND INTERIM S DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 DATE AND TIME OF NECROPSY: 02 14/91 8:15 PROSECTON: DENNIS 14OFFMAN TERMINAL 6ODY WEIGHT: 489 POST-FIX WEIGHER: NOT AVAILAB(E PATHOLOGIST: DR. TOM PAL14ER RECORDER: JILL BEVAN WEIGHER: VAL QUINN ------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN WEIGHT RATIO 0 na -------------------- -------- -------- -------- STA ------ BRAIN 0 LIVER IL71 2.0662 .4222 % 1.0000 WEIGHT LUNG u LF. TE Ti I TEI 19.8089 1.4s$2 1.620S 1.6179 4.0476 % .2980 % .3311 % O.SI71 Iosl .7143 WEIGHT WEIGHT WEIGHT AT. TEST .3306 % -------AC-C-ES-S-/-SE-X---0--G---S-O-)--------------3-.-56-6-6-------------------.-72-88 % .7830 1.7202 WEIGHT WEIGHT ------------------------------------------------------- ORGAN NAME PA T"0 L 00 Y KEYWORDS. SAEV*ERAITY. DISTRIBUTION ---------------------- 0 0 SE NV A T10 N S A &* GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOAIC fl --------------------------------------------------------------------------------------- KIDNEYISI IKO) . . . LIVER (Lil GROSS: -iAiGi OE@ViSilEil MICRO: -PRESENT . . . . . . . ;.- - -- BiLATiltAL' * * ' * ' ' * * * * * * ' * . . * -DILATATION.PELVIC ... ... . ... . .. .. .. . .. .. . .. ... . . ... .. . .. .. . .. .. .. ... MICRO: -MINIMAL -"YPENTROPHY "EPATOCELLULAR: CHARACTERIZED BY A CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MORE "OHMENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLTIMP' APPEARAN THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OT"ERW THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON.-- - - - - - - - - - - - - - BRAIN (OR). COAGULATING GLD ICOI. LF. TESTIS ITEO). LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE ISVI, URETHRA ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. pp -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?$IS SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF BEATH:003F425@91 DATE AND TIME EC OPSY: STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH; 14 (2S/91 8:11 PROSECTON: JACK SERFORT TERMINAL 6ODY WEIGHT: 529. POST-FIX WEIGHER: NOT AVAILA0S3 E PATHOLOGIST: DR. TOM PALMER RECORDER: JOE M. KIZZIEE WEIGHER: STEVE VAN ADEST -------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN WEIGHT RATIO 0a a ------ STA T ------ BRAIN (841 LIVER (LIJ 2.1700 le.ts3o ---.4;99 % 1.0000 WEIGHT LUNG ILU) LF. TESTIS 1.6110 3.1835 % .3044 % 7.7606 .7427 WEIGHT WEIGHT AT. TESTIS ITTEE101 1.8900 1.8593 .3570 % .3512 % .1710 .8581 WEIO"T ACCESS/SEX 0 0 Sol 3.2008 .6216 % I.Si$4 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- OROAN NAME P A I H 0 L 0 0 r 0 0 S E N V A T 1 0 N S *a a KEYWORDS, ;E*VERITY.DISTRIBUTION O-E-NE-R-AL---C-O-MM-E-NT-----------------------------------------------G--R--O--SS---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-O-PA-T-H-OL-O-G-IC----FI-N------- LIVER ILI) 04'03CSI-:iLL iliS6Ei/61tdAiS'N6RiIAL . . . MICRO: --AlilkA@ "Y'PE,N T*,OP,HY "E *PA*TOC*E L*L,ULA,P C "AN, AC, TE*P Z E,D D'Y A, CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTES A MORE NOMOOENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARAN THIS "ENLARGED* APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-C-ROS-COP-IC - EX-A041-14A-TION-: - - - - - BRAIN ION). COAGULATINO OLD (CO). LF. TESTIS ITEO). LUNG ILUI. PROSTATE (PRI, SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. X401SON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMALNUMBER:Cg?ele DATE OF DEATH: 03/25/91 SIX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE AND TIME OF NECROPSV: STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 (25/91 1:41 PitOSECTON: JULIE A. LARSEN TERMINAL 6ODY WEIGHT: SIG. POST-FIX WEIGHER: NOT AVAILA003 E PATHOLOGIST: 04. TOM PALMER RECORDER: JOE It. MI zZIEE --------------------------------------------------------------------------------------W--E--I-G-H--E--R-: --S-T-E-VE---VA-N--A-DE-S-T----- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra -------------------BRAIN 6 -------2.1783 TO BODY WEIGHT -------.3712 % WEIGHT RATIO -------1.0000 STA T ------WEIGHT LIVER ILKII LUNG (LU) 26.19SS 1.8784 4.4441 % .2800 % 12.0257 WEIGHT RLTF.. TESTIS 2.0033 .3414 % .7705 .9197 WEIGHT WEIGHT TEI ACCESS/SEX 0 (1 ISO) 1,9311 3.7408 .3291 % .637S % .1065 WEIGHT ------- ---------------------------------------------- 1.7173 WEIGHT ORGAN NAME --- ------- GENERAL COMMENT PA T"0 L 0a Y KEYWORDS. ;EAVER*ITY. DISTRIBUTION 0 8 S E R V A T 10 N S a a 6 GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOOIC FIN ------------------------------------------------ GIO'Csl:-AL@ iliS6Ei/61tdAiS'N6Rk@ LIVER ILI) . . . . . . . . . . . . . . . . ... . . . . . . . . MICRO: :AMII'NNlt4AL.*FOCAL' INAL . . . . . .liFEA"TiOi.6itAN6L"T6Ui -HYPERTROPHY NEPATOCELLULAR: CHARACTERIZED BY A CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTES A WAC HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPIARAN THE FOLLOWING TISSUES WERE-UNREMARKABLE THIS 'ENLARGED* APPEARANCE. THE CELLS ARE OTHERW 6 -- ---- - AT 141CROSCOPIC EXAMINATION: - - - - - - - BRAIN JOR). COAGULATING GLD (CO). LF. TESTIS (TEO). LUNG ILU). PROSTATE (PA). SEMINAL VESICLE ISVI, URETHRA ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HMAAZDLIESTOONN. WLIASBCOORNATSOIRNIES U.SA.MAER.ICA, INC. pp -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7617 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHED LED SECOND INTERIM SA DATE OF DEATEH:00F2413/91 STUDY DAY OF DEATH: S$ STUDY WEEK OF DEATH: I DATE AND TIM ECROPSY: 02(13/91 7:24 PROSECTOlt: RUTH SCHMITZ TERMIUnAL iODY WEIGHT: 421. POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: DR. TOM PAL14EN RECORDER: KEVIN BILLINGS ---------------------------------------------------------------------W-E-I-GH-E-R-: --J-I-L-L --BE-V-A-N------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO GRAIN WEIGHT RATIO 0 R (I ST A T ------------------- -------- -------- -------- ------- BRAIN 1.9841 4027 % 1.0000 WEIGHT LIVER IOLRII LUNG LF. TELLTIUI T'E1O 22.5410 1.4213 I.Sole 5'.2801 % .3315 X .3712 % 11.4112 .7163 .8022 WEIGHT WEIGHT WEIGHT AT. TESTIS TE ACCESS/SEX JO ISO) 2.4218 .5641 % 1.2206 WEIGHT ------- ----------------------------3-.-6-04-4-------------------.-8-40-6--2--------------1-.-8-1-06-------------WE-I-G-HT---------------- ORGAN NAME ; Ita P A T H 0 L 0 0 V 0 0 S E N V A T 1 0 N S A * A KEYWORDS. EVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIN ---------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LI) GAOGCSI: -ALi iliS6Ei/6R6AiS'N&i4AE MICRO: .-i4lilhAE -HYPENTROPHY, HEPATOCELLULAR: *CHARACTERIZED*BY A CENTRILOBOJL TED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MAE 'PLt$MP* APPEARAN THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN [SRI. COAGULATING (ILD ICUP, LF. TESTIS ITEOI. LUNG ILU). PROSTATE (PRI, SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CSIGIS SEX: 14ALE DOSE GROUP: 4 DATE OF DEAIH:00F3422VI DATE AND 71ME EC OPSY: STUDY DAY OF DEATH: 93 STUDY (22/91 8:54 PROSECTOR I WEEK SACRIFICE STATUS: OF DEATH: 14 SCHEDULED TERMINAL SACRIFIC TERMINAL 6C)DY WEIGHT: S96. POST-FIX WEIOHER: NOT AVAILA0O3 E RUTH SCHMITZ PATHOLOCI S:T: DR. TOM PALMER RECORDER: JOE M. KIZZIEE --------------------------------------------------------------------------------W-E-I-GH-E-R-:--J-I-L-L--B-EV-A-N-------------------- OROAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORWOEAN TO BRAIN 0 a0 --------------------- -------- TO BODY WEIOHT 1%) -------- IGHT RATIO -------- SIA T BRAIN (OR) LIVER (LI) LUNG (LU) 2.1651 22.81$S 1,63S$ .3029 % 3.8241 % .2742 % 1.0000 10.5392 ------- WEIGHT WEIONT LF. TESTIS ITTEE?L AT. TESTIS 1.7618 .2053 % .75S4 .1137 WEIOHT WEIGHT ACCESS/SEX 0 0 SO) 1.7261 3,9577 .2894 % .797S WEIO"T ------------------------------------------------------------.-06-3-4--% --------------1-.8-2-8-0------------W-E-IG-H-T--T---------------- ORGAN NAME ; oba P A T H 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S h A A KEYWORDS. EVERITY. DISTRIBUTION ---------------------- OROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND GENERAL COMMENT JOCI ---------------------------------------------------------------------------------------- LIVER ILI) G OS : -ALi iliS6Et/6R6AkS'N61tkA@ . M.IC.RO.: .-S.LI.GH.T. . F.OC.AL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -MINIMAL -INFLAMMATION,ARAMULONATOUS -HYPENTROPHY HEPATOCELLULAit: CHARACTERIZED BY AN CENTRILOSULATED PATTEIN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CY OPLASH AND THE CELL @OftDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEAIANC THIS 'ENLARGED' APPEARANCE, THE CELLS ARE OTH NWI THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATI-ON:- - - - - - - - - - - - - - - BRAIN (BA). COAGULATING OLD (CO). LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANT14ALNUMBER:Ce?elo DATE OF DEATH: 01 24491 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SC EDULED FIRST INTERIM SACI STUDY DAY OF DEATH: 36 STUDY WEEK OF DEATH: 6 DATE AND TIME OF 4EC OPSY: 01(24/91 8:44 PROSECT01t; JULIE A. LAITSEN TENRMINAL IODY WEIGHT: 409. POST-FIX WEIGHER: NOT AVAILAO E PAT14OLOGIST: DR. TOM PALMER RECORDER: VAL QUINN WEIGHER: DEBBIE PIRKIL ----------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 pa 0 ----------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TAT -------- BRAIN a LIVER Li LUNG ILU) 2.0962 26.3509 .5123 % 6.4390 % 1,0000 12.S701 WEIGHT T WEIGHT T LF. TESTIS 1.5621 .3817 % .7452 WEIGHT I ItT. TESTIS ITTIJOI 1.5730 I.Sot? .3140 % .3907 % Isol .7627 WEIGHT T WEIGHT 7 ACCESS/SEX Oto ISOI 2.4021 5870 % --------------------------------------------------------------------- I.IASO WEIGHT T ------------------------------------------------ ORGAN NA14E P A T N 0 L 0 0 Y , 0 8 S E ItV A T 1 0 N S * 0 Is KEYWORDS. ;E*VER&ITY. DISTRIBUTION -GE-N-ER-A-L ---CO-M-ME-N-T ----------------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T --C-O-M-ME-N-T-S ---/---H-IS-T-O-PA-T-H-OL-O-G-IC----F-IN-D------- LIVER ILI) 0100CSI:'-iLEitiS6Ei/6R6AiS*N6Ri4AE . . .. .. . .. ... .. . ... ... . ... .. .. . .. .. .. . .. . .. .. .. . .. .. .. . ... . .. .. .. MICRO: -MINIMAL. MI)LTZ-FOCAL -INFILTRATES.MONONUCLEAR CELL -MINIMAL -"YPENTROPHY "EPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATED PATTERN 110 WHICH THE "IEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE "PLtN4P* APPEARANC THIS 'ENLARGED" APPEARANCE. THE CELLS ARE OTHERWI - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - -I - - - - - - - ------BR-A-I-N--(-O-N-).--C-O-AG-U-L-A-TI-N-G--O-L-D--(-C-G-).--L-F-.--T-ES-T-I-S--I-T-EO-)-.--L-O-NG---IL-U-)-.--PR-O-S-T-AT-E---IP-R-I-,--S-EM-I-N-A-L --V-ES-I-C-L-E --(-SV-I-.--U-R-ET-H-R-A----------------- -sib at%.n INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR --------------------------------------------------------------------------------------------------------------------- AN114ANLUMBER:C$7620 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACA DATE OF DEATH: 01/25/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 (25/91 0:01 PROSECTON: DENNIS HOFFMAN TEITMIHAL BODY WEIGHT: 469.8 POST-FIX WEIGHER: NOT AVAILAoOl E PATHOLOGIST: ON. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-EI-G-H-ER-:---P-AT-H-O-L0-4-3-Y --D-EP-A-RT----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0R 0A --------------------- -------- -------- WEIGHT RATIO -------- S TA T BRAIN ILBINJ LIVER 2.OIS4 4290 % 1.0000 LUNG ILU) Lf. TESTIS olTEOIISOI 25.3547 1.4847 l.so6a 5:3969 % .3160 % .3207 % 12.SSOS .7367 .747S WEIGHT T WEIGHT T AT. TESTIS TE ACCESS/SEX 0 1.4394 3.1823 .3084 % .7142 WEIGHT 7 WEIGHT T ------------------------------------------------------------.8-7-74--%---------------1.-5-79-0-------------W-EI-G-H-T--T---------------- ORGAN NAME P A TH0 L0 0 Y 0 9 S ER VA T 10N S KEYWORDS. ;E&VEARITY. DISTRIBUTION AA A O-E-ME-N-AL---C-O-MM-E-NT--------------------------------------------------G-R-O-SS---F-RE-E--T-E-X-T--C-O-M-ME-N-T-S ---/---HI-S-T-OP-A-T-HO-L-O-CI-I-C--F-I-N-D------- GAGOCSI:-ALC iliS6Ei/61tdAiS'N6RkAE ' * . . ... . . . . . . . . . . . . . . . . . ... . . . . . . . LIVER (LI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -SLIGHT. FOCAL -SLIGHT -INFLAMWTION.GRANULO$4ATOUS -HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED BY AN CENTITILODULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AMD THE CELL BORDERS WOUNDED GIVING THE CELLS A MORE "PLUMP* APPEARANC THIS 'ENLARGED' APPEARANCE, THE CELLS ARE OTHERWI THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN (OR). COAGULATING OLD (CG). LF. TESTIS (TEO). LUNG ILU). PROSTATE IPR), SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------------------------- cn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67621 DATE OF DEATH: OF03426191 SEX: MALE DOSE (INOUP: 4 STUDY DAY OF DEATH: 97 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICI DATE AND TIME ECROPSY: (26/91 7:39 STUDY WEEK OF DEATH: 14 PROSECTOR I PlItKEL TERMINAL 6ODY WEIGHT: 607. POST-FIX WEIGHER: NOT AVAILA0B3 E PATHOLOO S:T:DEBDBRI.E TOM PAL14EN ITECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-I-GH-E-R-:--J-O-E--M.--K-I-Z-ZI-E-E----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE 70 BODY WEIGHT (%I ORGAN TO BRAIN 0 Aa A ---------------------BRAIN 1L8IJ -------- -------- WEIGHT RATIO -------- S TA I -------- LIVER 2.2076 2S.0527 .3637 X 1.0000 WEIGHT I LUNG ILU11 1.7479 4.1273 % .2180 % 11.3414 .7918 WEIGHT TI LF. TE T RT. TESTIS TEI 1.0589 .2733 % .7514 WEIGHT WEIGHT 7 ACCESS/SEX 0 G Sol 1.630S 2.4959 .2686 % .4112 % .7316 1.1306 WEIGHT T -----------------------------------------------------------------------------------------------W-EI-G-HT---T---------------- ORGAN NAME P A T H 0 L 0 0 Y, 0 B S E R V A T 1 0 N S a * a KEYWORDS. ;EAVERITY. DISTRIBUTION G-E-NE-R-AL---C-O-MM-E-NT-----------------------------------------------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-WN-T-S----/---"-IS-T-O-P-AT-H-O-LO-G-I-C---F-IN-D------- LIVER ILI) GA0O2S1: -AL@ iliS6Ei/i)NdAiS'N6RkA@ .... . . . . -THE- FO-LLOW-ING- iql6lt6:' HI,NNl,MA*L,*F*OCA,L, :Ml INAL -- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MICROSCOPIC -"YPERTROPHY NEPATOCELLULAR: C14ARACTERIZED BY AN CENTRILOBULATED PATTERN IN W141CH THE HEPATOCYTES A MORE "OMDOENEOUS CYTOPLASM AND THE CELL BORDER$ ROUNDED GIVING THE CELLS A MORE 'PLUMP* APPEARANC - - - - TH-IS -'EN-LAR-GED"- A-PPE-ARAN-CE.-- T-HE -CEL-LS -ARE- OT-HER-WI EXAMINATION: BRAIN (BRI. COAGULATING OLD ICGI, LF. TESTIS (TEO), LUNG ILU). PROSTATE IPRI, SEMINAL VESICLE ISV). URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER. C67622 DATE OF DEATH: SIX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE AND TIME STUDY OF054Elc7R/901ps0y5:(17/91 DAY OF DEATH: 149 STUDY 7:25 PROSECTOR I T RUTH WEEK OF DEATH: SCHNITZ 22 TERMINAL 6ODY WEIGHT: S63.4 POST-FIX WEIGHER: NOT AVAILAO PAT14OLOO ; DR. TOM PALMER RECORDER: JOE N. KIZZIEE W-E-I-GH-E-R-: ---J-A-CK---SE-R-F-O-R--T -.- -- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R0A --------------------7 -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- S TA T -------- BRAIN JLOIRJ LIVER 2.IGSS .3736 % 1.0000 WEIGHT T LUNG ILU) 21.3009 I.S222 3.1794 % .2701 % 10.1161 WEIGHT I IF. TESTIS RT. TESTIS ITTEEDll 1.1721 2.0001 .3322 % .7230 .8891 WEIGHT T WEIGHT T ACCESS/SEX 0 a Sol 3.17se .35so % 6177 % .$SO3 WEIGHT T -----------------------------------------------------------------------------------------1.-1-4-0-8------------W-E-IG-H-T ---I------ ORGAN NAME KEYWORDS. T H 0 I 0 0 Y- 0 0 S E R V A T 1 0 N S EVERITY.-DISTRIBUTION ------- ----------- ------- ORoSS-FREE-TEXT-COMMENTS--/--HISTOPAT"OLOGIC-FlIID GENERAL COMENT 01'0(S:I:-AL@ -iliS6Ei/6R6AiS*N6lti4Ai .. . . . . . . . . . . . . . . . . . . . . . . . . THE BFORLLAOIWNINGTISSUESWEREUNREMARKOAL E AT M -C1R OSCOPIC EXAMINATION: - - - - - - - - - - - - - - URETHR@L an()Ukl COAGULATING OLD (C(l).IF. TESTIS (TEO). LIVER (LI). LUNG (LUI. PROSTATE (PRI. SEMINAL VESICLE ISV --------------------------------------------------------------------------------------------------------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA MHAADZILSEOTONN, WLIASBCOORNASTIONRIES U.SA.MAER.ICA. INC. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL HU14BER: C$7623 DATE OF DEATH: 03 91 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL AODY WEIGHT: DATE AND TIME OF 4t2sc4opsy:03(25/91 8:58 PROSECTON: JULIE A. LARSEN 470. RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILAR t PATHOLOGIST: 04. TOM PALMER WEIGHER: STEVE VAN ADEST -------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (ORAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I ORGAN TO BRAIN WEIGHT RATIO 0Ra S TA T ---------------------- -------- -------- -------- ------- BRAIN IL1O1R 2.1070 3:4474 % 1.0000 WEIGHT LIVER 11.9421 oil$ % l.slss WEIGHT LUNG (LU) LF. TESTIS TE I.S$22 1.6761 .3301 % .3561 % ISO$ .795S WEIGHT WEIGHT 07. TESTIS TEI0 ACCESS/SEX 010 ISO) 1.9628 3.773S .3533 % .8017 % .7192 WEIGHT -------------------------------------------------------------------------------1-.7-9-0-9------------W-E-IG-H-T ----------------- P A T H 0 L 0 0 Y' 0 B S E R V A T 1 0 N S O-R-GA-N---MA-K-E -------------K--E-Y-W-OR-D-S-.--;-[-*V-E-AR-IT-Y-.--D-IS-T-R-IB-U-T-IO-N----------O--N--O-S-S---F-RE-E---TE-X-T--C-O-M-ME-N-T-S-a-A-/-*--"-IS-T-O-PA-T-H-O-LO-G-I-C ---F-IM------GENERAL COMMENT LIVER (LI) GI(OICSI-:ALi iliS6Ei/61tdAiS*N6R*MAL. . . .... ... .. .. . .. .. .. . .. .. MICRO: -MINI14AL . .. .. . -HiPiltilt6PiY' 'HE*PA*TOC*EL'L'ULA*R''C:'HAR'AC'TE*R*IZE'D'BA'*Yt CENTAILOGULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANC THIS 'ENLARGED' APPEARANCE, THE CELLS ARE OTHERWI THE- FO-LLO-WING- -TISS-UES -WE-RE-UNRE-MARK-ABLE- - AT- MICROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - - - BRAIN IGN). COAGULATING OLD ICO), LF. TESTIS (TEO). LUNO (LU). PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. p -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67624 SEX: MALE DOSE GROUP: 4 DATE Of DEATH: 03 26491 STUDY DAY OF DEATH: 97 DATE AND TIME OF 4EC OPSY: 03/26/91 7:55 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: 536. POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: STEVE VAN ADESTINE PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN ---------------------------------------------------------------------------------WE-I-G-HE-R-:---GA-I-L--M-. --NO-V-A-K ----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 nG ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T BRAIN 8 LIVER ILIII 2.1104 .3932 % 1.0000 ------WEIGHT LUNG (LU) 26.4S96 1.4814 4.9301 % 12.5377 WEIGHT LF. TESTIS RT. TESTIS TT"tOl 1.7078 .2760 % .3182 % .7020 .8092 WEIGHT WEIOHT ACCESS/SEX 01(i ISO) 1.7SS4 3.3164 .3271 % .8311 WEIGHT ------------------------------------------------------------.-6-31-0--%--------------1-.-6-04-6-------------WI-E-IO-H-T ---------------- ORGAN NAME P A I N 0 L 0 0 Y 0 8 S E R V A T 10 N S KEYWORDS. SEVERITY, DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENTS HISTOPAT"OLOGIC FIN GENERAL COMMENT JOCI ------------------------------------------------ G OS : -A.LL. T.IS.SUE.S/.OR.GAN.S . .NO.RM.AL . . . . . ..... . . . . . . . . . . . . . . . . . . . . . . . . . LIVER (LI) A16R6:*:MIN IMAL.'FOCAL . . . . SLIGHT -INFLAMMATION.GRANULONATOUS -HYPERTROPHY NEPATOCELLULAR: CHARACTERIZED BY A CENTAILOBULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAN THIS 'ENLARGED" APPEARANCE, THE CELLS ARE OTHERW THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN ION), COAGULATING GLD (CO). LF. TESTIS (TEO). LUNG ILO). PROSTATE (PR). SEMINAL VESICLE ISVI, URETHRA ------------------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COIS25 DATE OF DEATH: 01/25/91 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 0:22 POTOSECTON: JULIE A. LARSEN TE TMINAL 6ODY WEIGHT: 371 POST-FIX WEIGHER: NOT AVAT LA01(2E5191 PATHOLOGIST! DR. T014 PAL14ER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-IG-H-ER-:---P-A-TH-O-L-OG-Y---D-EP-A---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 pa ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA ------ BRAIN LIVER ltelill 2.1277 S732 % 1.0000 WEIGHT LUNG LUl@ 20.1581 5.430S % 9.4741 WEIGHT LF. TELT TEO1 RY. TESTISx 0 I[ 1'.'665131S .4207 % .4454 % .7340 .7771 WEIGHT WEIGHT ACCESS/SE la ISO) 1.8745 2.6636 .4511 % .7170 WEIGHT ------------------------------------------------------------7-1-76---% ---- 1.2SIO WEIGHT ----------------------------------------------- ORGAN NAME P A T H 0 L 0 a Y' 0 8 S E R V A T 1 0 N S & a a KEYWORDS. SEVERITY, DISTRIBUTION -------------- GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl GENERAL COMMENT ----------------------------------------------- GI'OCSI: -AL@ fliS6E@/dRdAiS*N6RI*4A*L LIVER ILI) . . . . . . . . . . . . . . . . . . . . .. .. .. . .. .. . ... .. . MICRO: -MINIMAL -"YPENTROPHY "EPATOCELLULAR: CHARACTERIZED BY CENTNILOBULATED PATTERN IN WHICH THE NEPATOCYTE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BOODE ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARA THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTHER T "E F0 LLOW INO TISSUES WERE-UNREt4ARK- ABLE AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - -- - - - - - - ------B--R-A-IN--- -(-ON-)-.--C-O-A-GU-L-AT-I-NG-----O-L-D -- (-C-O-I-,-- L-F-.--T-E-S-TI-S--- (-T-E-O-)-.--L-U-NG---(-L-U-I-.--P-R-OS-T-AT-E----I-P-R-)-.--S-E-MI-N-A-L--V-E-S-I-CL-E---(-SV-I-.---UR-E-TH-R-A---- INDIVIDUAL APPENDIX D ANIMAL PATMOLOOY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7026 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST POST-RECO DATE OF DEATH: 05/16/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 141 STUDY WEEK Of DEATH: 22 1 7:58 PROSECTON: DEBBIE PINKEL TERMINAL 6ODY WEIGHT: 560 POST-FIX WEIGHER: NOT AVAILA0B5(lE a/9 PATHOLOGIST: 04, TOM PALMER RECORDER: JOE H. KIZZIE --------------------------------------------------------------------------------W-E-I-GH-E-R-: --J-A-C-K --S-ER-F-OR-T------------------ ORGAN NAME ABSOLUTEonaAWHEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra ---------------------- (GRAMSI -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- STA ------ BRAIN I LIVER (aL"il 2.0641 .3685 % 1.0000 WEIGHT LUNG ILU) 11.6121 1.6345 3.3224 % .2911 % 0.0171 .7919 WEIGHT LF. TES I NT. TESTTISS ITTEEIOI 1.1775 I.Ioss .33SI X .9090 WEIGHT WEIO"T ACCESS/SEX 0 0 SO) 4.2949 .3384 % .7667 X -------------------------------------------------------------- .9113 2.0101 WEIGHT WEIGHT r ---------------------------------------------------- ORGAN NAME PA T N0 L00 Y KEYWORDS, ;E*VERITY. DISTRIBUTION ---------------------- 0 8 S EN VA T 10N S *A 0 GROSS FATE-TEXT COMMENTS / HISTOPATHOLOCIIC Fit GENERAL COMMENT --------------------------------------------------------------------------------------- 0100CSI:-ALi itiS6Ei/61tdAiS'N6RilAi - -THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON- - - - - - - - - - - - - - - - BRAIN IONI) COAGULATING OLD (CO). LF. TESTIS (TEO). LIVER (LIJ. LUNG (LUI. PROSTATE IFNI, SEMINAL VESICLE (S URE fitit Uhl -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67627 DATE OF DEATH: 01/23/91 SEX: 14ALE DOSE (IROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST I TERIM SAC STUDY DAY OF DEATH: 35 DATE AND TIME OF NECROPSY: 01(23/01 8:37 STUDY WEEK OF DEATH: 5 TER14INAL 6ODY WEIGNT: 306. POST-FIX WEIGHER: NOT AVAILAB E PROSECTOR: DENNIS HOFFMAN PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------W-E-I(-I-H-ER-:--G-A-IL--M-.--N-O-VA-K----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSO ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra -------------------- -------- TO BODY WEIGHT IXI -------- WEIGHT RATIO -------- S--T--A--T- BRAIN ( LIVER (OLRil LUNG ILU) 2.0441 20.7728 1.510S .5159 % 5.2430 1 1.0000 10.1623 WEIGHT WEIGHT LF. TE Ti RY. TESTISS ITTEEOIJ 1.5123 .3112 % .3817 % .7390 .7391 WEIGHT WEIGHT ACCESS/SEX 0 a (SO) 1.5975 .4032 % .?$is WEIGHT -------------------------------------2.-S-7-01--------------------.6-4-1-7--% --- 1.2573 WEIGHT -------------------------------------------- ORGAN NAME ; * 0 P A T N 0 L 0 0 Y. 0 0 S E R V A T 1 0 N S KEYWORDS, EVERITY, DISTRIBUTION ---------------------------------------------------------G-R-OS-S FREE-TEXT COMMENT; */* HISTOPATHOLOGIC FIN GENERAL COMMENT IGCI: ------------------------------------------------ LIVER ILI) os -AL@ iliS6E/6RdAiS'N6RAA@ . .. .. .. . .. .. .. . . . ... .. . .. .. .. .. .. .. .. . .. .. . .. . .. .. .. .. . .. .. MICRO: -SLIGHT -HYPERTROPHY HEPATOCELLULAR: CHARAdTiltiZiD*B*Y A' CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTES A 14ORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CILLS A MORE -PLUMP' APPEARAN THIS 'ENLARGED' APPEARANCE, THE CELLS ARE OTHERW TH F LLOWI(NOGR,TIS UE WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON: BRAIN . COAGULATING GLD ICU). Lf. TESTIS ITEO). LUNG (LU). PROSTATE IPRI. SE14INAL VESICLE (SVI, URETHRA ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67628 DATE OF DEATH: OS/16/91 SEX: 14ALE DOSE GROUP; 4 SACRIFICE STATUS: SCHEDULED. FIRST POST-RECOV DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 148 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: 666. P-O-S-T--F-I-X---WE-I-G-HE-R-:---N-O-T--A-V-A-T-L-A0O-5(-lE-e-/-91----7-:-0-1--------PPA-RTO-HS-OEL-COT-GO-IRS-:T-:--J-U-LDI-RE.---AT.-OM--LA-PRA-SL-EMN-ER------------------R-E-C-O-RD-E-R-:---J-OE---N.--K-I-Z-Z-IC-E-------- WEIGHER: JACK SERFORT ORGAN NAME ABSOLUTE ORCIAN WEIGHT ORGAN WEIGHT RELATIVE OROAN TO BRAIN 0 Ra ---------------------- IGRAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T BRAIN LIVER (BRI (L 2.2129 21.4S94 .3320 % 3.2197 % 1.0000 0.6974 ------WEIGHT LUNO ILU) LF. TESTIS EO11 1.7619 I.SGSI .2644 % .2798 % .7962 .1428 WEIOHT WEIGHT WEIGHT RT. TESTIS TTE ACCESS/SEX 0 0 SOI 1.8650 4.4556 .2800 % .6085 % .8432 2.0135 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 (1 Y' 0 8 S E R V A T 1 0 N S KEYWORDS. ;E*VERITY. DISTRIBUTION ---------------------- ---------------------------------------G-R-O-SS---F-R-EE---T-EX-T----CO-M-M-EN-T-;---*-/-*--H-IS-T-O-P-AT-"-O-LO-G-I-C----F-I-NE---- GENERAL COMMENT JOCI . . . . . . . . . . . . . . . . . ..... . . . . . . . . . . . . . . . . . . . . . . . . . 0 OS : -ALL TISSUES/ONGANS NORMAL LIVER (LI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -SLIGHT. MULTI-FOCAL -NECROSIS.COAGULATIVE THE- FO-LLOW-ING - -TISS-UES - WE-RE -UNR-E14A-RKAB-LE - AT- MI-CROS-COPI-C - EX-AMTH-ATIO-N: - - - - - - - BRAIN (OR). COAGULATING OLD ICO). LF. TESTIS (TEO). LUNO ILU), PROSTATE IPRI, SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------------------------- APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- AN104AL NUMBER: CS?629 SEX: 14ALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM S DATE OF DEATH: 02/14/21 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 DATE AND TIME OF NECROPSY: 02 14/91 7:16 PROSECTOR: RUTH SCHNITZ TERMINAL 13ODY WEIGHT: 505 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-I-GH-E-R-: --V-A-L--Q-UI-N-N-------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Na ---------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA ------ BRAIN ION) LIVER ILI) 2.1037 27.170S .4164 % 1.0000 WEIGHT LUNG ILU) 1.5019 5.3712 % .2973 % 12.OISS .7139 WEIGHT WEIGHT LF. TESTIS RT. TESTIS TEI 1.8013 1.19ss .3S$B % IS63 WEIGHT ACCESS/SEX 0 a ISO) 2.IS63 .37S2 7L .5654 % .9010 1.3S7$ WEIGHT WEIGHT ------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 0 Y 0 8 S E A V A T 10 N S 0 & 0 KEYWORDS.;E*VERITYD.ISTRIBUTION -------------------------------------------------------- GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl GENERAL COMMENT c 7 ---- --------------------------------------------- LIVER (Lil Ol'oost:-ALE iliS6Ei/6R6AiS*N6ltkAE . . . . .... .. .. .. .. .. . .. .. .. . .. .. . .. .. .. . .. .. . .. .. .. .. .. MICRO: -MINIMAL -HYPERTROP"Y HEPATOCELLULAR: CHARACTERIZED BY A CIENTITILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAN THIS_'ENLARGED' APPEARANCE. THE CELLS ARE OTHERW -- ---- --- - TH-EF-OLL-OW-IN-TGIS-SU-EWS-ERE-UN-RE-MAR-KA-BAL-TEM-ICR-OS-CO-PEI-XCAM-IN-AT-ION- BRAIN(OR).COAGULATIONLGD (CO).LF.TESTIS(TEO).LUNG(LU).PROSTATFEPRI,SEMINAVLESICLE(SVI,URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67630 DATE OF DEATH: SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED. FIRST INTERIM SA DATE AND 71ME OF0142E5C/R9O1PSY: STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 PROSECTON: DENNIS HOFFMAN TERMINAL BODY WEIGHT: 457 POST-FIX WEIGHER: NOT AVAILA01(2t5/91 8:42 PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-IG-H-E-R:---P-A-TH-O-L-OG-Y---D-EP-A---------------- OROAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R0 ---------------------BRAIN JOR) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA ------ LIVER ILI) 2.0441 27.OSII .4474 % 5.9193 % 1.0000 13.2292 WEIGHT LUNG ILU) LF. TESTIS OITEOI 1.5123 1.63SG .3309 % .7396 WEIGHT WEIGHT RT. TESTIS TEI 1.6673 .3579 % .3648 % .7999 WEIGHT ------A-C-CE-S-S-/S-E-X----0---I-S-O-)--------------2.-9-9-9-1-------------------.6-5-6-3--% --------------1.-.4-161-65-74------------WW-EE-IIGG-HH-TT ----------------- ORGAN NAME KEYWORDS. *SEV*ERAITY. P ADISTTRHIBU0TILON0 0 Y* 0 0 S E R V A T 1 0 N S A A & ----------------------------------------------------------G--R--O--S-S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-H-O-LO-G-I-C ---F-t ------- GENERAL COMMENT JOCI . . . . . . . . . . . . . . . . . ..... . . . . . . . . . . . . . . . . . . . . . . . . . LIVER ILI) G OS : -ALL TISSUES/ORGANS NORMAL -SLIGHT -HYPERTROPHY. HEPATOCELLULAR: CHARACTERIZED BY A CENTRILOSULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLUMP* APPEARAN THIS "ENLARGED" APPEAltANCE,. THE CELLS ARE OTHERW THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - - BRAIN IBR), COAGULATING GLD ICO). LF. TESTIS ITEO). LUNG ILU). PROSTATE IPR). SEMINAL VESICLE (SVI. URETHRA ------------------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67631 SEX: 04ALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE OF DEATH: 01/23/91 STUDY DAY Of DEATH: 35 STUDY WEEK OF DEATH: 5 TERMINAL 600Y WEIGHT: 381. DATE AND TIME OF NECROPSY: 01/23/91 9:39 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL BEVAN P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-O-T -A-V-A-IL-A-B-LE-----------------P-A-TH-O-L-OG-I-S-T:---D-R-.--T-ON--P-A-L-14-E-R---------------W-E-I-GH-E-R-:--G-A-I-L--M.--N-O-V-AK------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT OROAN TO BRAIN 0Ra ---------------------- -------- -------- WEIGHT RATIO -------- S TA T ------- BRAIN ILBIJ 2.1312 SS92 % 1.0000 WEIGHT LIVER 22.4129 5.0811 % lo.sies WEIGHT LUNG ILU) LF. TESTIS T. TESTIS I TTEE10 1.4438 1.4198 1.405S .3789 % .3126 % .:?Is . 002 WEIGHT WEIGHT A ACCESS/SEX 0 0 ISO) 2.9983 .3018 % .7047 % es9s 1.4069 WEIGHT WEIGHT ------- ----------------------------------------------------------------------------------------------------- ORGAN NAME KEYWORDS. ; A * P A I H 10L 0 0 V, 0 8 S E R V A T 1 0 N S EVERITY. DISTRIBUTION ikh,ft ----------------------------------------------------------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-1-4O-L-O-GI-C---F-IN------- GENERAL COMMENT LIVER (Lll -THE- FO-LLO-WING- GIGOCSI: -ALi iliS6Ei/6ft6AiS'N6Ri4AE ... .. .. ... .. . .. .. .. . .. .. . MICRO: -MINIMAL, MULTI-FOCAL -liFiLiNATiS:M6N6N6CEEA*'CiLE -MINIMAL -HYPENTROPHY HEPATOCELLULAR: . .. CHARACTERIZED BY A CENTNILOBULATio PATTERN IN WHICH THE 14EPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLUMP" APPEARAN - - - - - - - T-HIS -'EN-LAR-GED'- A-PPE-ARAN-CE.-- T-HE -CEL-LS -ARE- OT-HEA-W -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MICROSCOPIC EXAMINATION: ------B-R-A-IN---JO-R-)-.--CO-A-G-U-LA-T-I-N-G--O-L-D--(C-O-)-.--L-F.--T-E-S-T-IS--(-T-E-O-)-. -L-U-N-G--I-L-U-),--P-R-O-ST-A-T-E--I-P-R-),--S-E-M-IN-A-L--V-E-S-I-CL-E--(-S-V-I-,--UR-E-T-H-RA----------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA MHAADZILSEOTONN, WLIASBCOORNATSOIRNIES U.SA.MAE.RICA. INC. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67632 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE OF DEATH: 01/23 ol STUDY DAY OF DEATH: 35 STUDY WEEK OF DEATH: S TERMINAL 6ODY WEIGHT: 316. DATE AND TIME OF NEC40PSY: 01(23/91 I:S3 POST-FIX WEIGHER: NOT AVAILAS E PROSECTON: DENNIS HOFFMAN PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------W-E-IG-H-E-R-: --G-A-I-L --M.--N-O-V-AK------------------ ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMSI ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN WEIGHT RATIO 0 Ra ---------------------- -------- -------- -------- STA T ....... BRAIN ILGIRJ LIVER 1.9220 11.2926 .4973 % 4.7329 % 1.0000 9.SI75 WEIGHT WEIGHT LUNG ILU) 1.35S4 .3SO7 % .7052 WEIGHT LF. TESTIS TEO RT. TESTIS ici 1.5971 I.Si$3 .4132 x .3928 % .9310 .7900 WEIGHT WEIGHT ACCESS/SEX 010 ISO) 2.39S3 .6197 % 1.2453 WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T " 0 L 0 0 Y ' 0 0 S E N V A T 10 N S a a & KEYWORDS, ;E@EARITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOAIC FIM ------------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER ILI) GIGOCSI:-ALi iliS6Ei/6*6AiS*"6Ri4A@ . ... ... .. .. .. .. . ... . .. .. . .. .. . .. .. .. . .. .. . . MICRO: -SLIGHT -HYPERTROP"Y NEPATOCELLULAR: C"ARA@TiNiZiD'B*Y A' CENTRILOBULAirED PATTERN IN WHICH THE "EPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLUMP" APPEARAM THIS "ENLARGED" APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN JOR). COAGULATING OLD (CO), LF. TESTIS (TEOI. LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE ISVI, URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. Pit --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7033 SEX: MALE DOSE GROUP: 4 DATE OF DEATH: 00F 142S/91 STUDY DAY OF DEATH: 37 DATE AND TIME ECROPSY: tA01(2S/91 1:30 SACRIFICE STATUS: SC EDULED FIRST INTERIM SACR STUDY WEEK OF DEATH: 6 TE"0MINAL 6ODY WEIGHT: 4S3.7 POST-FIX WEIGHER: NOT AVAI 0 E PROSECTOR I NANCY DIEDRICH PAT"OLOO S:T: 00. TOM PALMER RECORDER: JILL BEVAN -------------------------------------------------------------------------------W-E-IG-H-ER-:---P-A-TH-O-L-OG-Y---D-EP-A-RT----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 P0 A -------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- STA T BRAIN JLOIRJ LIVER 2.1741 .4792 % 1.0000 ----T- LUNG TELLUI 2 S.6081 1.7s94 5.6443 % .3878 % 11.7717 .80 3 WEIGHT T LF. TI RT. TESTIS 1 TEIISO) 1.7336 .3821 X .7997 4 WEIGHT T WEIGHT T ACCESS/SEX 0 G 1.7207 3.28SO .3783 % .7240 % .7915 WEIGHT T --------------------------------------------------------------------------------------I-.-S-l-i-o------------WE-I-GH-T---T-------- ORGAN NAME KEYWORDS. ;E&VER*ITYP. ADISTTRHIBU0TILON0 0 Y. 0 8 S E A V A T 1 0 N S a 6 a K-I-D-N-E-Y-(-S-)--(-K-O-)---.--.--.---.-.--.--.--.-.--.--.--.-.--.--.--.-.--.--.--.-.-.-.-.-.--G--R--O-S-S---F-RE-E---TE-X-T--C-O-W-MN-T-S----/---H-IS-T-O-PA-T-H-OL-O-GI-C----F-IN-D------- GROSS: -LARGE PELVISIES) 'ItiGAT'ji0i"'KiDiEiS*C6LiE6Tit)i LIVER ILI) MICRO: -PRESENT -DILATA ION,PELVIC . M.IC.RO:. . .. .. -MINIMAL. . .. FOCAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MINIMAL -INFILTRATES.MONONTPCLEAR CELL -HYPERTROPHY HEPATOCELLUTAN: CHARACTERIZED BY AN CENTAILOSULATED PATTERN IN WHICH THE HIEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE *PLTIMP" APPEARANC SPLEEN ISP) . . . . . . . . . . . . . . . . . . . . . . . . . THIS 'ENLARGED' APPEARANCE-, THE CELLS ARE OTHERWI GROSS: -RAISED FOCUSII)/AREA(S) MICRO: -UNREMARKABLE :AiOiG'MiRdli:*SiNdLi iE6 AN6 iAi iliM*AiEA i i i COLLECTED - - - - - - - - - - - - -HISTOPATHOLOGICAL COMMENT: )NO MICROSCOPIC CORRELATE TO GROSS OBSERVATION. - --- -- --- --- --- -- -- -- APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER; C67633 SEX: 14ALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE OF DEATH: 01/25/91 STUDY DAY OF DEATH: 37 DATE AND TIME OF NECROPSY: 01(25/91 1:30 STUDY WEEK OF DEATH: 0 TE RMINAL 6ODY WEIGHT: 453. POST-FIX WEIGHER: NOT AVAILAO E PROSECTOR: NANCY DIEDRICH PATHOLOGIST: DR. T014 PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------WE-I-G-HE-R-: --P-A-T-HO-L-O-GY---D-E-PA-R---------------- THE FOLLOWINGTISSUESWERE UNNE14ARKABLAET MICROSCOPICEXAMINATION: BRAIN (ON).COAGULATINGOLD (CO).LF. TESTIS ITEOI.LUNG (LU).PROSTATE(PRI.SEMINALVESICLE ISVI.URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- AN104AL NUMBER: C67634 DATE OF DEATH:002412 91 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SAC DATE AND TIME F STUDY DAY OF DEATH: SS EC40PSY: 02(12/91 9:24 PROSECTOI STUDY WEEK OF DEATH: I NUTH.SCHNITZ TERMINAL 6ODY WEIGHT: 47S.1 POST-FIX WEIGHER: NOT AVAILAS E PAT"OLOCI S:T: DR TOM PALMER RECORDER: DENNIS MYFNAN -------------------------------------------------------------------------------WE-I-GH-E-R-: ---PA-T-HO-L-O-GY---D-E-PA-R-T---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 A0 A ---------------------BRAIN JORP -------2.1173 TO BODY WEIGHT (Z) -------.4604 % WEIGHT RATIO -------110000 S TA T -------WEIGHT T LIVER (LI) LUNG (LU) 24.6474 I.SOS4 S.1871 % .3169 % 11.2004 WEIOHT T LF. TESTIS OT. TESTIS I TTEE01 1.7292 1.8230 .3040 % .8182 .7906 WEIGHT T WEIO"T T ACCESS/SEX 0 0 SOI 3.0144 .3037 % .0492 % .8334 1.4101 WEIOHT T -----------------------------------------------------------------------------------------------W-E-I-O"-T---T --------------- OROAN NAME P A T H 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S % d. a KEYWORDS. ;EAVE'RITY. DISTRIBUTION -------------------------------------------------------- OROS$ FREE-TEXT COMMENTS / HISTOPATHOLOOIC FIND GENERAL COMMENT 7 --- ------------------------------------------------- GAGOCSI:-ALi iliS6lEi/6RdAhS'N6ltkAE . ... . . . . . . . . . . . . . . . . . . . . . . . . . . LIVER ILI) LUNG (LU) 'HE"PA'TOC'EL'LU*L*A**:C*HARACTERIZED'BY AN CENTAILOBULATED PATTERN IN WHICH THE "EPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANC . . . . . . . . . . . . . . . . . . . . . . . . . . .TH.IS. '.EN.LA.RGE.D". .AP.PE.ARA.NC.E.. THE .. CELLS . .. ARE .. OTHERWI . . GROSS: NECROPSY NOTE: )TRIMMING COMMENT: LUNG SECTION 14ISSING FROM WET T MICRO: -TISSUE MISSING OF TRIMMING THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON.-- - - ------BRAIN (OR). COAGULATING OLD ICGI. LF. TESTIS (TEO), PROSTATE (PRI. SEMINAL VESICLE ISVI, URETHRA (URI ----------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?635 DATE Of DEATH: 03/24/91 SEX: MALE DOSE GROUP: 4 STUDY DAY OF DEATH: 97 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFI DATE AND TIME OF NECROPSY: 03/26/91 9:40 STUDY WEEK OF DEATH: 14 PROSECTOR: STEVE VAN ADESTIME TE RHINAL 6ODY WEIGHT: 544 POS7-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN -----------------------------------------------------------------------------W-E-I-G-HE-R-:--J-O-E--1-4.--KI-Z-Z-IE-E---------------- ORGAN NAME - ------------ ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGNT RATIO -------- 0 Ra S TA ------ BRAIN 10L4IJ LIVE 2.OS95 .3780 % 1.0000 WEIGHT LUNG ILU) 20.2688 1.5667 3.7197 % 9.8416 WEIGHT LF. TESTIS RT. TESTIS 1.891501 .2875 % .34so % .7607 .9129 WEIGHT WEIGHT TEI ACCESS/SEX 0 a ISO) 71 31-0409 .3S93 % .9506 WEIGHT ------------------------------------------------------------s-s-li---% --------------1.-4-7-6-S ------------W-EI-G-HT----------------- ORGAN NAME * % a P A 7 H 0 L 0 0 Y. 0 B S IEP V A lr1 0 N S A 0 A KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl - ----------------------------------------------------------------------------------------------------- GENERAL COMMENT c LIVER ILI) 01(olsi:-ALi iliS6Ei/61tdAiS*N61tkAE .. .. ... .. . .. .. .. ... . . ... . .. .. . . ... .. . MICRO: :HlHINAL. FOCAL -INFILTRATES,MONONUCLEAR . .. .. .. . .. . CELL MINIMAL -HYPERTROPHY "EPATOCELLULAR: CHARACTERIZED BY CENTRILODULATED PATTERN IN WHICH THE HEPATOCYTE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL OORDE ROUNDED GIVING THE CELLS A MORE *PLTIMP' APPEARA THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHER THE- FO-LLO-WING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN IBRI, COAGULATING GLD ICGI, LF. TESTIS (TEO), LUNG fLU), PROSTATE IPRI, SEMINAL VESICLE ISVI. URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67636 SEX: 14ALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE Of DEATH: 01/25/91 STUDY DAY OF DEATH: 37 DATE AND TIME OF NECROPSY: 01(2S/91 7:45 STUDY WEEK OF DEATH: 6 TERMI MAL 6ODY WEIGHT: 315. POST-FIX WEIGHER: NOT AVAILA E PROSECTOR: DENNIS HOFF14AN RECORDER: JACK SERFORT ---------------------------------------------P-A-T-HO-L-O-GI-S-T-:----DR-.---TO-M--P-A-L-ME-R------------------W-EI-G-H-ER-:----P-AT-H-O-LO-G-Y---D-E-PA-R-------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0n a ---------------------- -------- TO BODY WEIGHT (1) -------- WEIGHT RATIO -------- S TA T ------- BRAIN (OR) LIVER (LI) 2.0247 21.9217 S25S % s.619S % 1.0000 10.8271 WEIGHT WEIGHT LUNG ILU) LF. TESTIS OITEI1I3S.. 1.5187 1.5013 .3942 % .3196 % .?Sol .74is WEIGHT PT. TESTIS TE 1.4497 .3783 % .7160 WEIGHT WEIGHT -------A-CC-E-SS-/-S-EX------0---------------------2-.4-7-9-6---------------------.0-4-3-6--%---------------1-.-2-2-47--------------W-E-IG-H-T--------- P A T H 0 L 0 0 Y. 0 8 S E P V A T 1 0 N S ORGAN NAME ---------------------- K-E-Y-W-OR-D-S-. ---;-EA-V-E-RI-T-Y-.--D-I-S-TR-I-B-UT-I-O-N-----------ONOSS FREE-TEXT COMMENT; */* "ISTOPATHOLOGIC FIN OEMENAL COMMENT ------------------------------------------------ LIVER ILI) (31'03CSI-:iL@ iliS6Ei/6R6AiS*N6Ri4AE .. ... . .. ... . .. .. .. .. .. . .. . MICRO: -MINIMAL -Hipinin6p,HY, *HE*PAT,OC,EL*L,ULA*#:,C,HAR*AC,TE,R,IZE,D,BY*A* CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER WOUNDED GIVING THE CELLS A MORE 'PLt$MP' APPEARAN THIS "ENLAROID' APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLOW-INO- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATIO-N: - - - - - - - - - - - - - - - BRAI-N---(B-R-I-,--C-O-AG-U-L-mA-TI-N-G---O-L-D--I-C-OI-,---LF-.--T-E-S-T-IS---(-T-E-OI-,---LU-N-G---(L-U-)-.--P-R-O-ST-A-T-E---(-P-RI-.--S-E-M-I-N-AL---V-E-SI-C-L-mE------(S-V-I-.--U-Rm E-T-H-R-A-- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?637 DATE OF DEATH: 05/16/01 SEX: MALE DOSE 13ROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 141 1-43 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: 529. PDST -FIX WEIGHER: NOT AVAT LA0O5L/16/91 PROSECTOR: DEBBIE PIRKEL RECORDER: JOE M. KIZZIEE ---------------------------------------P-AT-H-O-LO-G-I-ST-:---O-N-.--T-OM--P-A-L-ME-R----------------W-E-IG-H-E-R:---J-A-C-K --SE-R-F-OR-T--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT IGRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 R0 - -------------BRAIN oni -------- TO BOD-Y--W-E-IG-H-T- 1%) WEIGHT RATIO -------- STA T ------- LIVER ILI 2.1437 22.6290 .4045 x 1.0000 WEIGHT LUNG LU11 E, LF. TEIT OJT 1.6194 4,2704 X .3118 % 10.5560 .7811 WEIGHT WEIGHT PT. TESTIS TEI0I@O, 1.5730 1.6060 .2060 1 .7339 WEIGHT -------ACCESS/-S-EX-----0--------------------3.-3-4-5--1-- - - - - .3031 % .6313 % .7492 1.5604 WEIGHT WEIGHT ORGAN NAME -P A- T- "- -0 -L 0- 0- Y-'- - -- -- - -- -- --0-9-S-E-R-V-A-T-1-0-N-S---;-;-; KEYWORDS, ;E*VER*ITY. DISTRIBUTION -------------------- -----------------------------------------------------?-R-OS-S FREE-TEXT COMMENTS / "ISTOPATHOLOOIC FIM GENERAL COMMENT 0400CSI:-ALi iliS6Ei/6R6AiS*N6Rt@Ai .. . .. .. .. .. .. . .. . .. .. I. . - - - - - - - - - - - - - - - - - - - - - - - --------------------------------------------------------------- THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BURRAEITNHR JOR()U*lCOAGULATING GLD (CGI. LF. TESTIS (TEOI. LIVER (LIJ, LUNG (LUI. PROSTATE (PRI, SEMINAL VESICLE (S -------- ----------------------------------------------------------------------------------------------------------- 00 INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C97838 SIX: MALE DOSE GROUP: 4 DATE OF DEATH: 02/13/91 DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH; 56 3/91 9:00 STUDY WEEK OFSACDREIAFTIHC:E I STATUS: SCHED LED SECOND INTERIM SAC TERMINNAL 6ODY WEIGHT: 488.9 POST-FIX WEIOHER: NOT AVAILA0O2(El PROSECTOR:, TJULIE A. LARSEN RECORDER: KEVIN BILLINGS - ---------------------------------P-A-TH-O-L-OO---S--:--DR-.--T-O-M--PA-L-M-ER-----------------WE-I-G-HE-R-:---JI-L-L--B-E-VA-N-------------------- OROAN NAME ABSOLUTE ORGAN WEIGHT IONAMS) ORGAN WEIGHT RELATIVE OOGAN TO BRAIN 0 P0 A ------------------- -------- TO BODY WEIGHT (1) -------- WEIGHT RATIO -------- STA T BRAIN JLOIRJ LIVER 2.1911 .4412 % 1.0000 LUNG,,ILU, 26.s4os 1.6116 S.4216 % 12.1120 WEIGHT T LF. TIS OITEOIISOI RY. TESTIS TE 1.4647 .3298 % .2996 % .73ss seIs WEIGHT T WEIGHT T ACCESS/SEX 0 1.4910 3.2086 .30so x .6805 WEIGHT T ------- ----------------------------------------------.-05-6-3--% --------------1-.4-8-4-4------------W-E-IG-H-T--T--------------- ORGAN NAME - ------------KEYWORDS, GENERAL COMMENT P A T " 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S a Is ;E*VER*ITY,-DISTRIB-U-T-IO-N----------GROSS FREE-TEXT COMMENTS HISTOPATHOLOGIC FINDI -------------------------------------------------- LIVER (LI) Ol'OOCSI-:iLi itiS6Ei/6R6AiS'N6Nl4A@ SLIGHT -liFiLiNATiS*m6N6N6cEEAit'CiLi -HYPENTItOP14Y "EPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTES A A NNE HOMOGENEOUS CYTOPLASM AND THE CELL 13ORDERS ROUNDED GIVING THE CELLS A MDRE *PLUMP" APPEARANCE THIS 'ENLARGED* APPEARANCE, THE CELLS ARE OTHERWIS THE FOLLOWINGOR, TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION.BRAIN ( ----------------.--CO-A-G-U-L-AT-I-N-G--O-L-D --IC-O-)-.--LF-.--T-E-S-TI-S---(T-E-O-I-.-L-U-N-G--I-L-U)-.--P-R-O-ST-A-T-E--(-P-R-).--S-E-M-I-NA-L--V-E-SI-C-L-E--(-S-V-I-, -U-R-E-T-H-RA---(U---------------- gn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7632 DATE OF DEATH: 02/13/91 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED SECOND INTER114 SA DATE AND TIME OF NECROPSY: 02 STUDY DAY OF 13/91 8:10 DEATH: S$ PROSECTOlt: STUDY WEEK OF DEATH: DENNIS HOFF14AN I TERMINAL 6ODY WEIGHT: 479. P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-O-T -A-V-A-IL-A-R-(E-----------------P-AT-H-O-LO-G-I-ST-:---D-R-.--T-OM--P-A-LM-E-R----------------WR-EE-ICG-OHR-EDR-E:-R:--3-1K-EL-VLI-N-BE-VB-AI-NL-LI-N-G-S ---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R a ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S IA T BRAIN (ON) LIVER (LI) 2.0544 .4219 % 1.0000 LONG (LU) 26.8281 1.4064 S.8009 % .2936 % 13.0581 .6846 WEIGHT LF. TESTIS IT NT. TESTIS TEI0 1.9760 2.0156 .412S % .9618 WEIGHT WEIGHT ACCESS/SEX 0 0 isol 3.2112 .4208 % .6704 % .9811 1.5631 WEIGHT WEIGHT -------------------------------------------------------------------------------------------------------------------- O-R-GA-N---NA-M-E -------------K-EYWORDS. ; a Is P A T " 0 L 0 0 Y' 0 0 S E P V A T 1 0 N S A a Ak EVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC FIN GENERAL COMMENT --------------------------------------------------------------------------------------- LIVER (LI) GAOOCSI: -iLi iliS6Ei/6ft6AiS'N61ti4A@ . . . . . ... . . . . . . . MICRO: -AlilhA@ -"YPENTROP"Y' "EPATOCELL6LAlt*: *CHARACTERIJIE*D*BY A CENTRILODULATED PATTEAN IN WHICH THE "EPATOCYTES A NONE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ITOUNDED GIVING THE CELLS A MORE 'PLUMP' APPEATAN THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - - 13RAIN (OR). COAGULATING OLD (CO). Lf. TESTIS (TEO), LUNG (LU). PROSTATE IPA). SEMINAL VESICLE ISVI. URETHRA ------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. M.A.D.IS.O.N., ..WI.S.C.ON.S.IN....U..S...A........-.-.-.-.-.------------------------------------------------------------------P-A----------- ANIMAL NUM13ERC:O?640 DATE OF DEATH: 01/24/91 SEX: MALE DOSE GROUP: 4 STWY DAY OF DEATH: 38 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE AND TIME OF NECROPSY: 01 24/91 7:22 STUDY WEEK OF DEATH: 6 PROSECTON: KEVIN BILLINGS TERMINAL IODY WEIGHT: 397. POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: VAL QUINN --------------------------------------------------------------------------------W-E-IO-H-E-R:---D-E-BB-I-E--P-I-N-KE-L------------------ ORGAN NA14E ABSOLUTE ORGAN WEIGHT 10itAMS1 ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Aa ---------------------- -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- S TA T -------- BRAIN (ON) LIVER (LI) 1.9166 23.9939 SO04 % 1.0000 WEIGHT T LUNG (LU) LF. TESTIS 1.3627 6.0438 % .3432 % 12.0779 .6459 WEIGHT T WEIGHT T RT. TESTIS I 7TEE10 1.55$S 1.5153 .3926 % .3993 % .784S .7980 WEIGHT T ------A-C-CE-S-S-/S-E-X---0--G--I-S-O-I --------------2-.0-1-12--------------------.7-3-3-3 --%--------------1-.4-e-ss-------------WW-EEI-GIHGT-H-T--7-T --------------- ORGAN NA14E P A T H 0 L 0 0 Y' 0 9 S E A V A T 1 0 N S * a a KEYWORDS. ;EVAER'ITY, DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND GENERAL COMMENT ------------------------------------------------- LIVER (LI) GAGOCSI:-ALi fliS6Ei/6N6AiS'N6Ri4Ai . 1.41.CRO.: .-S.LI.GHT. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -"YPENTROPHY HEPATOCELL6LAN': .. . . . . . . 'C'HAR'AC'TE'#I'ZEB**DY AN CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES LUNG (LU) A MORE HOMOGENEOUS CYTOPLASM AND THE CELL @ORDERS ITOUNDED GIVING THE CELLS A MORE PLUMP* APPEARANC . . . . . . . . . . . . . . . . . . . . . . . . . THIS 'ENLARGED* APPEARANCE. THE ELLS ARE OTHERWI GROSS: NECROPSY NOTE: iTilkKit466OAkNi:' CUi(l'N6T'F6UiM'li itei iliS6E'A MICRO: -TISSUE MISSING TRIMMING. THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE AT MICROSCOPIC EXAMINATION: BRAIN (80). COAGULATING OLD ICO). LF. TESTIS (TEO), PROSTATE (PA). SEMINAL VESICLE (SVI. URETHRA (URI -------------------------------------------------------------------------------------------------------------------- 14 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67641 DATE OF DEATH: 03/22/91 SEX: t4ALE DOSE GROUP: 4 STUDY DAY OF DEATH: 93 SACRIFICE STATUS: SCIIEDULEID TERMINAL SACRIFIC DATE AND TIME OF NECROPSY: 03 22/91 7:57 STUDY WEEK Of DEATH: 14 PROSECTOR: NUT" SCHNITZ TERMINAL 6ODY WEIGHT: 545. POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------------------- OROAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 Ra --------------------- -------- TO BODY WEIGHT (ZI -------- WEIGHT RATIO -------- S TA T ------- BRAIN JLOIRJ LIVER 1.9354 3s49 % 1.0000 WEIGHT T LUNG ILU) 24.7112 1.2912 4:S321 % 12.7716 WEIGHT T LF. TESTIS (TEO) RT. TESTIS I Ell I.S70S .2307 % .2896 % .6671 .8161 WEIGHT T WEIGHT T ACCES! SO) 1.6706 2.S436 .3082 % .4604 % .8632 WEIGHT T -------------------------------------------------------------------------------1.-3-1-4-3 ------------W-EI-G-HT---T---------------- ORGAN NAME P A T H 0 L 0 0 Y@ 0 0 S E R V A T 1 0 N S a e a KEYWORDS, ;EOVERITY. DISTRIBUTION ----------------------------------------------------------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-"-O-LO-G-I-C ---F-IN-D------- OENERAL COMMENT LIVER ILI) GI(OICSI: -iLE iliS6Ei/61tdAiS'N6Ri4AE . M.ICR.O:. .-M.IN.IM.AL.. .FO.CA.L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -MINIMAL -INFILTRATES.MONONUCLEAR CELL -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATED PATTEITN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE "PLtN4P' APPEARANC THIS *ENLARGED" APPEARANCE. THE CELLS ARE OTHERWI - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - --- - - - - - - -------B-R-A-IN---I-BR), COAGULATING OLD fCO). LF. TESTIS ITEO). LUNG ILU), PROSTATE IPRI. SEMINAL VESICLE ISVI. URETHRA I -------------------------------------------------------------------------------------------------- 00 co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. p ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?642 DATE OF DEAYH: 02/12/01 SEX: MALE DOSE GROUP: 4 STUDY DAY OF DEATH: 55 SACRIFICE STATUS: SCHED LED SECOND INTERIM S DATE AND TIME OF NECROPSY: 02 12/91 7:31 STUDY WEEK OF DEATH: I PROSEC701t: KEVIN BILLINGS TERMIUNAL 1ODY WEIGHT: 504 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER RECORDER: DENNIS HOFFMA WEIGHER: PATHOLOGY DEPA ----------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO GRAIN 0 Ra ---------------------- -------- TO BODY WEIG14T 1%) -------- WEIGHT RATIO -------- S TA ------ BRAIN 8 LIVER ILIRI 2.ISOS .4261 % 1.0000 WEIGHT LUNG (LU) 30.2508 S.9938 % 14.0669 WEIGHT LF. TESTIS OITEOISOI 1.4758 1.7243 .2924 % .3410 % .6863 .1011 WEIGHT NT. TESTIS TEI ACCESS/SEX 0 1.7436 .34SS % .8108 WEIO"T WEIGHT -------------------------------------3.-0-7-60--------------------.8-0-9-8-%---------------1.-4-3-0-1 ------------W-EI-G-HT------------------ ORGAN NAME P A T N 0 L 0 (kY. 0 0 S E 0 V A T 1 0 N S a a ob --------------------K--E-Y-WO-R-DS-.---;-E-AV-E-R-'I-T-V.--DI-S-T-RI-B-U-TI-O-N----------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl GENERAL COMMENT ------------------------------------------------ LIVER ILI) GA(OICSI-:ALE iliS6Ei/61tdAiS*N6Ri4AE . 1.41.CRO.: .-M.IN.IMA.L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -HYPENTROPHY. HEPATOCELLULAR: CHARACTERIZED BY A CENTRILODULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLUMP* APPEARA THIS 'ENLARGED* APPEARANCE. THE CELLS ARE OTHERW THE FOLLOWING TISSUES WERE UNNE14ARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (OR). COAGULATING OLD (CGI. LF. TESTIS ITEO). LUNG ILUI. PROSTATE (PR). SEMINAL VESICLE (SVI. URETHRA ----------------------------------------------------------- co INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7643 SEX: MALE DOSE GROUP: 4 DATE OF DEATH: 03/2S/91 STUDY DAY OF DEAT14: 96 SACRIFICE STATUS: SCHEDULED STUDY WEEK OF DEATH: 14 TERMINAL SACRIFICE DATE AND TIME OF NECROPSY: 03/2S/91 POST-FIX WEIGHER: NOT AVAILABLE S:S3 PROSECTON: JACK SEITFORT TERMINAL 6ODY WEIGHT: 45S.6 RECORDER: JOE H. KIZZIEE PATHOLOGIST: DR. T014 PALMER ----------------------------------------------------------------------------------------WE-I-G-HE-R-:---S-T-E-VE---V-AN---AD-E-S-TI-------- OROAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 A (IA ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- S TA T BRAIN (881 2.0837 .4s?4 % -------- LIVER (Lll LUNG ILU) 17.6129 1.7374 3.06$9 % 1.0000 8.4S2? WEIGHT T WEIO"T T Lr . TESTIS 1.7180 .3813 % .1331 WEIGHT T RT. TESTIS ACCESS/SEX ITTEE? 1.7410 .3771 % .3823 % .824S .1359 WEIGHT T 0 G ISO) ------------------------------------- 3.7702 -- -- - -- -- - - -- - .827S % - ------- - - - - - - - 1-.80-94- WEIGHT I WEIGHT T -- - . - --- - - --------------------- ORGAN NAME PA T"0 L00 Y KEYWORDS. SEVERITY. DISTRIBUTION 0 B S E A VA T 1 0N S AA A ---------------------- ---------------------------------------GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC FIND GENERAL COMMENT IGCI ------------------------------------------------- LIVER ILI) a os : -A.LL. T.IS.SUE.S/.OR.GAN.S . .NO.RMA.L . . . . . ..... . . . . . . . . . . . . . . . . . . . . . . . . . GENERAL A16it6:'-iqlilitAE. . . . . . . . . . . . . . :HiPiRiR6PAY H'EPATOCELL6LAR- ChAiACTEIIIZED,BY AN CENTITILOBULATED PATTERN IN WHICH THE IIEPATOCYTES i A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS INFORMATION (XXI GROSS: ROUNDED GIVING THIS 'ENLARGED' . . . . . . . . ... . . . . . . . . . . "ECROPSY NOTE: )SMALL MOVEABLE THE CELLS A NNE 'PLTIMP" APPEARANCE APPEARANCE, . THE CELLS ARE OTHERWIS Ti@iS6E'MiSi liOiEb i**CEliltAE 6BiEi INADVERTENTLY OF NECROPSY NOT COMMENTED AS TO PRESENCE ON ASSE - -THE- FO-LLOW-ING - -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON- - - - - - - - - - - - - - - - - - --------B-R-AI-N---(-O-R). COAGULATING OLD ICOP. LF. TESTIS (TEO), LUNO ILU). PROSTATE (PRI, SEMINAL VESICLE ISVI. URETHRA JU ------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NTPMBER: C07644 DATE OF DEAT":OF02412/91 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SC"10 LED SECOND INTERIM SAC DATE AND TIME STTODY DAY OF DEATH: SS STUDY WEEK Of DEATH: I ECROPSY: 02 12/91 I:S2 PROSECTOR: KEVIN BILLINGS TERNIUNAL i ODY WEIGHT: 4S$.2 POST-FIX WEIGHER: NOT AVAILAO(C PATHOLOGIST: DR. TOM PAL14ER RECORDER: DENNIS HOFFMAN WEIGHER: PATHOLOGY DEPART --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN WEIGHT RATIO 0N 0A S TA T -------- -------- -------- BRAIN 1L0IJ LIVER 2.0121 21.SOIS .4411 % 1.0000 @ilG-"-T --TO- LUNG ILU) LF. TESTI 1.2S12 4.7132 % .2743 % 10.4802 .6218 WEIGHT TO WEIO"T TA NT. TESTISS ITTEE101 I.BSS2 1.8384 .4067 % .9220 WEIGHT TA ACCESS/SEX 0 0 SO) 3.22S2 .4030 % .7070 % 9137 1'8029 WEIO"T TA ----------------------------------------------------------------------------------------------------------W-E-I-O"-T---TA---- ORGAN NAME 0 a a P A T " 0 L 0 (1Y' 0 8 S E P V A T 1 0 N S aa KEYWORDS. SEVERITY, DISTRIBUTION ----------------------------------------------------------G--R--O--SS---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---"I-S-T-O-PA-T-H-OL-O-G-IC----FI-N-D-I------- GENERAL COM14ENT -JOCI LIVER ILI) GROSS: ALE fliS6Ei/6R6AiS*N6RAAE -MINI14AL -INFILTRATES.MONONTICLEAR CELL . .. .. .. -HYPENTROP"Y HEPATOCELLULAR: CHARACTERIZED BY AN CENTAILOBULATIEO PATTERN IN WHICH THE NEPATOCYTES A A MORE HOMOGENEOUS CYTOPLASM AND THE CELL @ORDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANCE THIS "ENLARGED' APPEARANCE. THE CELLS ARE OT"ERWIS THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - - -------B-R-A-I-N --I-B-R)-.--C-OA-O-U-L-A-TI-N-G--O-L-D--I-C-G-),--L-F-.--T-ES-T-I-S--(-T-EO-)-.--L-U-NG--(-L-U-)-,-P-R-O-S-T-AT-E--(-P-O-P-.--SE-M-I-N-A-L --VE-S-I-C-LE---I-SV-I-.--U-RE-T-H-R-A--(-U--------------- 4P- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- AN114AL 14UMBER: CO?64S DATE Of DEATH: OF03426/91 SEX: MALE DOSE GROUP: 4 STUDY DAY OF DEATH: 97 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE AND TIME ECROPSY: 03(26/91 8:53 STUDY WEEK OF DEATH: 14 PROSECTOR: DEBBIE PINKEL TER14INAL 6ODY WEIGHT: 621. POST-FIX WEIGHER: NOT AVAILAS E PATHOLOGIST: DR. TOM PALMER RECORDER: JILL BEVAN ------------------------------------------------------------------------------W-E-I-GH-E-R-:--J-O-E--N-.-K-I-Z-Z-IE-E----------------- OROAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSP ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I ORGAN TO BRAIN 0 Ra ---------------------BRAIN JOR) -------- -------- WEI-GH-T---R-AT-I-O S IA T ------- LIVER ILI) 2.2231 24.2040 .3578 % 1.0000 WEIGHT LUNG ILU) LF. TESTIS OITEO11 RT. TESTIS TE 1.6907 1.9167 2.0271 3.903S % .2720 % .3196 % 10.9111 .7603 .1934 WEIOHT WEIGHT WEIGHT ACCESS/SEX 0 (SO) 3.1797 .3281 % SIIS % .9115 WEIGHT -------------------------------------------------------------------------------1-.4-2-9-8------------W-E-IG-H-T ----------------- ORGAN NAME P A I H 0 L 0 0 Y' 0 8 S E R V A I 1 0 N S a a ik KEYWOftDS, ;E*VERITY, DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOCIIC FIN( GENERAL COMMENT ------------------------------------------------- LIVER ILI) GAOSSCI: -AL@ iliS6Ei/6RdAiS*NbRkAE . . . . .. .. .. . .. .. .. . ... . - . . .. .. ... . .. .. . .. . .. .. . . .. .. . .. . . . .. .. . MICRO: -MINIMAL -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED BY AN CENTITILOBULATED PATTERN IN WHICH THE NEPATOCYTE ----- ------ -- ---- - A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERSS ROUNDED GIVING THE CELLS A MORE -PLTIMP- APPEARANC THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTNERWI THE FOLLOWING TISSUES WERE UNREMARKABLE AT HICROSC 0Pt C E XAMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN ION). COAGULATING OLD (CO). LF. TESTIS (TEOI. LUNG ILUI, PROSTATE (PRI, SEMINAL VESICLE ISVI. URETHRA --------------------------------------------------------------------------------------------------------------------- rt'o)@ INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA MHAADZILSEOTONN. WLIASBCOORNASTIONRIES U.SA.MAER.ICA. INC. p ------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C$7646 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHED LED SECOND INTERIM S DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: S7 STUDY WEEK OF DEATH: 9 TERMINNAL iODY WEIGHT: 478 DATE AND 71ME OF NECROPSY: 14/91 8:29 PROSECTON: DENNIS HOFFMAN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVI%IL0A2B(E PATHOLOGIST: DR. YOM PAL14ER WEIGHER: VAL QUINN ------------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (ZI OROAN TO BRAIN WEIOHT RATIO 0 aa S TA ----------------------- -------- -------- -------- ------ BRAIN 1L8IJ LIVER 2.0739 .4333 % 1.0000 WEIGHT LUNG (LU) 24.2502 1.7329 S.0612 % .3921 % 11.6959 .83se WEIGHT WEIGHT LF. TESTIS OITEOJISOI NT. TESTIS TE 1.7144 1.7213 .3582 % .0267 WEIGHT ACCESS/SEX 0 3.1011 .3S$7 % .6480 x .8300 1.49s3 WEIGHT WEIGHT ------------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 0 Y- 0 0 S E N V A T 1 0 N S a KEYWORDS. ;E'V*ERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS ---------------------- it HISTOPATHOLOGIC Fl -------------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LI) GI(OICSI:-ALL iliS6Ei/6R6AiS*N6RkL MICRO: .-mlilka@ -HiPiRift6PAY' HIPATOCELLULAR: 'CHARACTERIZED'BY A CENTRILOBULATED PATTERN IN WHIC14 THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE *PLt#MP' APPEARAN THIS 'ENLAN(IED' APPEARANCE. THE CELLS ARE OT"ER@ THE- FO-LLO-WING- -TISS-UES- WE-RE-UNRE-MARK-ABLE- - AT- MICROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - BRAIN ION). COAGULATING OLD (CO). LF. TESTIS ITEO). LUNG (LU). PROSTATE (PR), SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. PA -------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C67847 SEX: 14ALE DOSE GROUP: 4 DATE OF DEATH: 02414/91 DATE AND TIME STUDY DAY OF DEATH: 57 SACRIFICE STATUS: SCHEDULED SECOND INTERIM $A STUDY WEEK OF DEATH: 9 TERMINAL 6ODY WEIGHT: OF ECROPSY: 02 14/91 7:30 POST-FIX WEIGHER: NOT AVAILAO(E PROSECTOlt: RUTH SCHNITZ 432. RECORDER: JILL BEVAN ------------------------------------------P-AT-H-OL-O-G-IS-T-:---D-R-. --TO-M--P-A-LM-E-R----------------W-E-IG-H-E-R:---V-A-L --QU-I-N-N--------------------- ORGAN MAKE ABSOLUTE ORGAN WEIGHT (GRANSI ORGAN WEIGHT RELATIVE TO 13ODY WEIGHT 1%) ORGAN TO BRAIN 0n a ---------------------BRAIN (OR) -------- -------- WEIGHT RATIO -------- S TA T ------- LIVER ILI) 2.1070 IO.SSS3 .4171 % 1.0000 WEIGHT LUNG ILU) LF. TESTIS I TTIE0E11 1.5717 4.S227 % .3633 % 9.2859 .7459 WEIGHT WEIOHT NT. TESTIS 1.6115 1.6969 .3741 % .7612 WEIGHT ACCESS/SEX 0 0 SO) 2.7296 .3923 % .6310 % IOS4 WEIGHT -------------------------------------------------------------------------------1-.2-9-5-5------------W-E-IG-H-T----------------- ORGAN NAME ; a e P A I H 0 L 0 0 Y* 0 9 S E A V A T 1 0 N S A a a KEYWORDS. EVERITY. DISTRIBUTION ----------------------------------------------------------O-R-OS-S FREE-TEXT COMMENTS / HISTOPAT"OLOGIC FIN GENERAL COMMENT ------------------------------------------------ LIVER (LI) (11'OCSI-:ALL ittSOEt/i)ltdAkS'N6RkAi . .. . .. . . M.IC.RO.: .-M.IN.IM.AL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED BY A CENTITILOSULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAM THIS "ENLARGED' APPEARANCE, THE CELLS ARE OTHERW THE- FO-LLO-WING-131tlT-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN I . COAGULATING OLD ICO). LF. TESTIS (TEO), LUNG (LUI. PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. pm -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67e4l SEX: 14ALE DOSE GROUP: 4 DATE OF DEATH:00F1424/91 DATE AND TIME ECROPSY: STUDY DAY OF DEATH: 36 /24/91 7:41 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: 392. POST-FIX WEIGHER: NOT AVAILAoBlLE PROSECTOR; KEVIN BILLINGS PATHOLOOIST: DR. TOM PALMER RECORDER: VAL QUINN ------------ABSOLUTE ORGAN WEIGHT ----- - ---- -------------------W-E-I-G-HE-R-:---D-E-BB-I-E--P-I-N--K-E-L- -ORGAN ORGAN MA14E ------------------- BRAIN (ON) (GRAMS) -------- WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEI-G-HT---R-AT-I-O 0R a S TA T ------- LIVER ILI) 2.1367 21.6322 s4sl % 1.0000 WEIGHT LUNG ILU) LF. TESTIS 1.4050 5.5184 % .3S$4 % 10.1241 SS76 WEIGHT WEIGHT PT. TESTIS 'ITE",? l.6sl4 I.Se?l .4213 % .7729 WEIGHT -------ACCESS/SEX 0 0 ISol 2.SOS3 .3008 % .6076 % .7334 1.2614 WEIGHT ----------------------------------------------------------------------------------WE-I-GH-T--------------- ORGAN NAME KEYWORDS. ;E*VER*ITYP. ADISTTRHIBU0TILON0 0 T 0 0 S E A V A T 1 0 N S A & a --------------------------------------------------------G-R-O-SS FREE-TEXT COW*NTS / "ISTOPATHOLOGIC FIN KIDNEYIS) (KDI . . . . . ------------------------------------------------ GROSS: -LAit0iOEiViSiEil' MICRO: -PRESENT :LiFi:'86TA 4OiLiCiE6 LIVER (LI) -DILATATION.PELVIC . ... .. ... .. .. . .. .. .. . .. . .. .. .. . .. ... . MICRO: -SLIGHT -HYPENTROPHY EPATOCELL6LAIt: 'ChAiAC*TE*R*IZE*D'B'YA' CENTRILOBULATE"D PATTERN IN W"ICN THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER! ROUNDED GIVING THE CELLS A MAE "PLUMP" APPEARANC THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTHERWI - -THE- FO-LLO-WINIG- -TISS-UES- WE-RE -UNN-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON.-- - - - - - - - - - - - - - - --------13RAIN OR). COAGULATING GLD ICOI, LF. TESTIS ITEO). LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE ISVI. URETHRA I ------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON, WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CO?649 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED. FIRST POST-RECOV DATE OF DEATH: STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 O,PSY: 05 17/91 7:07 PROSECTON: JULIE A. LARSEN TERMINAL BODY WEIOHI: 601. RECORDER: JOE N. KIZZIEE DATE AND TIME OFos4icgo POST-FIX WEIGHER: NOT AVAILAO(E PAT14OLOGIST: DR. TOM PALMER WEIGHER: JACK SERFOR7 ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIO"T RELATIVE ORGAN TO BRAIN 0 aa ORGAN NAME ----------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA T ------- GRAIN 16Ltli 2.1373 3SS3 % 1.0000 WEIGHT LIVER 17.4747 I.SIS3 2:9OS2 % .2519 % 1.1761 .7090 WEIGHT WEIGHT LUNG ILU) ED LF. TESTIS 17TEII 1.2237 .2034 % S72S WEIGHT RI. IE Sol 1.0322 4.0803 .3212 % .0784 % .9040 1.9091 WEIGHT WEIGHT ------A-C-CE-S-S-/S-E-X ---0-0--------------------------------------------------------------------------------------------------- P A T N 0 L 0 0 Y' 0 8 S E R V A T 1 0 N S O-R-GA-N---NA-M-E -------------K--E-Y-WIO-RD-S-,---;[-*-V-EoR-l I-T-Y.--D-I-ST-R-I-B-UT-I-O-N ----------G-R--O--SS---FR-E-E--T-E-X-T--C-O-M-ME-N-T-;--*-/-*--"I-S-T-O-PA-T-H-OL-O-O-IC----FI-N------ GENERAL CO'"' 04'OCSI:-ALi itiS6Ei/i)ltdAiS*N6Ri4Ai .. . .. . Lf. TESTIS (TEO) MIC116:'-1;06EiAiE' hUiTi-iO@A@ :AiR6PAY@DiOiNiRATiOh - - -- -THE FOLLOWING ---TISSUES WERE ---UNREMARKABLE ---- AT MICROSCOPIC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - - BRAIN (BRI. COAGULATING GLO ICQI. LIVER (LI). LUNG (LUI. PROSTATE IPRI, SEMINAL VESICLE ISVI, URETHRA JUN) --------------------------------------------------- ---------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. PR -------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C670SO DATE OF DEATH-,03F 422/91 SEX: t4ALE DOSE GROUP: 4 STUDY DAY OF DEATH: 93 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE AND TIME ECROPSY: 03(22/91 9:37 STUDY WEEK OF DEATH: 14 PROSECTOR: RUTH SCHMITZ TERMINAL 6ODY WEIGHT: SSO, POST-FIX WEIGHER: NOT AVAILAS F PATHOLOGIST: DR. TOM PALMER RECORDER: JOE N. KIZZIEE WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ON43AN WEIGHT RELATIVE ORGAN TO BRAIN 0 na ---------------------- -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- STA T ------- BRAIN LIVER IL1 2.1319 21.2076 .3132 1 1.0000 WEIGHT LUNG LUI 1.7301 5.0706 % .3110 % 13.2312 Ills WEIGHT WEIGHT IF. TEII I T NT. YES tiS E 1.7603 1.7022 .3164 % .3060 % .1257 WEIGHT ------A-C-C-ES-S-/--SE-X--.-1-0T-E-lo-l-s-o,--------------3-.-44-8-8-------------------.-6-20-0--%--------------1-..-87-1918-74------------WW-EE-IIGG-HH-TT---------------- ORGAN NAME KEYWORDS. ; 0NS - -------------------------E-V-ER-I-T-Y,---DI-S-T-R-IB-U-T-IO-N----------G-R-OS-S FREE-TEXT COMMENTS HISTOPATHOLO(31C FIW GENERAL COMMENT ------------------------------------------------- LIVER (LI 0100CSI:-ALL iliS6Ei/61tdAiS*N6lti4AC . 1.41.CR.O: .-M.IN.IMA.L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED BY AN CENTAILOBULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS MOUNDED GIVING THE CELLS A MAE *PLUMP* APPEARANC --- T"IS_'ENLAItOED* APPEARANCE. THE CELLS ARE OTHERWI -- --- ---- --- THE F;LLO-WINIGB-R I-TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON--. -------B-RAIN . COAGULATING OLD ICGI. IF. TESTIS (TEO). LUNG ILU), PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA I -------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA PRI HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: Cg?$Sl SEX,. 14ALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV TERMINAL 6ODY WEIGHT: 614.9 DATE Of DEATH: OS 17491 STUDY DAY Of DEATH: 149 STUDY WEEK OF DEATH: 22 PROSECTOR: RUTH SCHNITZ RECORDER: JOE H. KIZZIEE DATE AND ITKE OF 4EC OPSY: POST-FIX WEIOHER: NOT AVAI LA 7:41 PATHOLOGIST: DR. TOM PALMER WEIGHER: JACK SERFORT ----------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WIEIGHT RELATIVE ORGAN TO BRAIN 0R0 A ORGAN NAME -(-G-R-A-MS-)-- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- S TA T ........ --------------------BRAIN IBLNli 2.139S .3479 % 1.0000 WEIGHT T LIVER 20.?GOS 1.62$S 3.3762 % .2648 % 9.7034 .7412 WEIGHT T WEIGHT 7 LUNG ILU) LF. TESTIS 1.1001 .2929 % .8417 WEIGHT T al. IESTIS TE 1.772S .2183 % .5291 % .8285 I.S208 WEIGHT T WEIGHT T ------A-C-CE-S-S-/S-E-X ---ol-l-G-E-ol-l-s-ol----------------3-,--2--53-7---------------:------------------------------------------------------ P A T" 0 L 0 0 Y 0 8 S E N V A T 1 0 N S & A 2 ORGAN MA14E K-E-Y-WO-l-t-t)-S-.-;-E-GV-E-'R% -IT-Y-.--DI-S-T-RI-B-U-TI-O-N----------G--R-O--S-S---F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---"-IS-T-O-P-AT-"-O-LO-G-I-C---F-IW------- ---------------------- KIDNEYIS) IKO) ..... .RI.GH.T; . BOTH COLLECTED (IR6Si:'-iAiGiOEiViS' -SEt4lFLUTO MAIIEESRIAL-PELVIS -RIGHT: FILLED WITH CLEAR FLUID MICRO: -PRESENT -DILATATION.PELVIC LIVER ILII .. .. .. ... .. . .. .. .. toiCioiSiiOiE: iTilAMilt6@044iNi:'OiE'$iCtl6*'4 AT TIME OF TRIMMING @l@Ei AliSiPI6iit - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNN-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN JOR). COAGULATING OLD ICO). LF. TESTIS (TEO). LIVER ILI). LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE (S URETHR tuft) APPENDIX 0 INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI 0-4-A-DI-S-O-N,--W-I-SC-O-N-SI-N---U-.-S-.A-.------------------------------------------------------------------------------------------------ ANT04ALNUMBER:CGI$52 SIX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE DATE OF DEATH: 03/25/91 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMI AL IODY WEIGHT: SOG.3 DATE AND TIME OF NECROPSY: 03(25/91 9:01 PROSECTOR: JACK SERFORT RECORDER: JOE H. KIZZIEE POST-FIX WEIGHER: NOY AVAILAS E PATHOLOGIST: DR. 7014 PALMER WEIGHER: SIEVE VAN ADESTI --------------------------------------------------------------------------------------------------------------------- ABSOLUTE OROAN WEIGHT ORGAN WEIGHT RELA71VE ORGAN TO BRAIN S0 I0 A0 IA O-R-GA-N---MA-1-4E-------------- (GRANSI -------- TO BODY WEIOHT -------- WEIGHT RATIO --- -------I- BRAIN 1L8li 2.0904 .4129 % 1.0000 WEIGHT LIVER 21.0118 4.1019 % 10.0203 WEIGHT I LUNG ILU) 1.5510 .3063 % .7420 WEIGHT LF. TESTIS I.IS29 .3060 % .8864 WEIGHT 7 AT. TE @TTEEl0l 1.8162 .3s#7 % .8688 WEIGHT I ACCESS/SEX 0 Sol 4.0136 ------- -------------------------------------- - - - - - -- .8099 % 1.953S WEIOHT I - ---- ----- - - - - -.S----;-;-;--------------------- P A 7 N 0 L 0 0 Y' 0 9 S E R V A 7 1 0 N OROAR NAME KEYWORDS. ;EOVER*ITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC FIND ------------------------------------------------------------------------------------------------------------- GENERAL COMMENT 0100CSI:-ALi iliS6Ei/6lt6AiS'N6Ri4Ai LIVER ILI) kl6R6: . . . . . . . . . . . . . . . :HiPiRiR6P4Y* 1'4f'PAY'O*CE'LL'ULA'CR'*":AN'A*CYE*R'IZE'D*O'YA" CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE *PLtP4P' APPEARANC THIS 'ENLARGED" APPEARANCE. THE CELLS ARE OT14ENWI THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- M-IC-RO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - -- - - - - - BRAIN IBRI. COAGULATING OLD (CO). LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE (SVI. URETHRA --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON WISCONSIN. INC. PR MADISON. WISCONSIN USA -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67653 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFIC DATE OF DEATH: 03/25/91 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TE RMINAL ODY WEIGHT: 592@ DATE AND TIME OF NECROPSY: 03 25/91 10:13 PROSECTON: JACK SERFORT RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILAB(E PAT14OLOGIST: DR. TOM PAL14EN WE11314ER: STEVE VAN ADEST ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OROAN NAME ---------------------- ((IRANS) -------- 0110AN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 pa STA I ------- BRAIN LIVER LUNG LUI LF. TELTI OITIO RT. TESTIS TEI 2.0977 21.1513 1.8177 1.8806 1.1049 -.3543 % 4.1730 x .3070 % .3186 1 .3150 X 1.0000 13-7571 .8065 .8994 .1190 WEIGHT WEIGHT WEIGHT WEIGHT WEIGHT ACCESS/SEX 0 Isol 3.3110 .5712 1 1.4122 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 Y 0 0 S E P V A T 10 N S OROAN NAME KEYWORDS, ;E*VE'RITY, DISTRIBUTION GROSS FREE-TEXT COMMENT; *lh HISTOPAT"OLOGIC FIN --------------------------------------------------------- ------------------------------------------------ GENERAL COMMENT LIVER (LIJ a ,Aocsl:-ALi iliS6Ei/6R6AiS'N6RAA@ . .. .. .. MICRO: -AlifitAi.'f6CAL* . :SLIGHT, FOCAL . . . . . . . . . . . .-I.N.NEFCI.RLTO-SRAI-STcE6-ASa-UM-LOANT.IOVNE.UCL-EA-R-CE-L-L. . . . . . . . . . MINIMAL -"YPEITTROO NEPATOCELLULAR: CHARACTERIZED BY A CENTRILOBUL"YATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE "PLUMP' APPEARAN GENERAL INFORMATION IXXI . . . . . . . . . . . THIS,-ENLARGED- APPEARANCE, THE CELLS ARE OTHERW GROSS: HiC*OiSi iOiE': iWARi-ilkE*LiSiOi iViNiRA'L'"'IND*L*EF*Tl'NO'TEDI'N'CL' OBSERVATIONS INADVERTENTLY NOT COMMENTED ON AS T PRESENCE AT TIME OF HICROPSY - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - C)- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON WISCONSIN, lf#C. PRI f4ADtSON, WISCONSIN USA --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$?$S3 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE DATE OF DEATH: 03/2S/01 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEAT"@ 14 TERMINAL 600Y WEIGHT: 592, DATE AND TIME Of RECROPSY. 03 2s/ol 10:13 PROSECTON, JACK SERFORT RECORDER: JOE M. KIZZtEt POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR, TOM PALMER W[IONER: STEVE VAN ADEST --------------------------------------------------------------------------------------------------------------- HE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (Oft).COAGULATING OLD fCO). LF. TESTIS (TEO). LUNG (LU), PROSTATE IPRI, SEMINAL VESICLE ISV), URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIIOAL NUt4lgER-.C07OS4 SEX: KALE DOSE GROUP- 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACO DATE OF DEATH: 01424/91 STUDY DAY OF DEATH: 36 STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: 333.5 DATE AMD 1114E OF ECROPSV: 1(24/91 8:11 PROSECTON: KEVIN BILLINGS RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILA0 E PATHOLOGIST: DR. TOM PALMER WEIGHER: DEBBIE PIRKEL ----------------I---------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- (ARAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 na i S--T--A--T-- BRAIN (BRI LIVER (LI) t.9896 21.1401 Sees % 6.3389 % L.0000 10.6253 WEIGHT T WEIGHT T LUNG (LU) 1.2542 .3161 % .6304 WEIGHT T LF. TESTIS 01,Tl0 So, RT. TESTIS TE I.S6?9 1.5519 .4701 % .46s3 % .7100 .7000 WEIGHT T WEIGHT I ACCESS/SEX 0 2.7S74 8268 % 1.38so WEIGHT T --------------------------------------------------------------------------------------------------------------------- P A T " 0 L 0 0 Y' 0 0 S t N V A T 1 0 N S 0 it@% ORGAN NAME KEYWORDS. *SEV*ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMRENTS / HISTOPATHOLOGIC FIN( -------------------------------------------------------------------------------------------------------------- GENERAL LIVER COMMENT GAGOCSI: -ALi ili,S6Ei/6R6AiS'N6ltkAi (LIJ . . . . . MICRO: -iLiOAT' -HYPEN7#OPHY NEPATOCELLULAR: CHARACTERIZED BY AP CENTRILOBULATED PATTERN IN WHICH THE "EPATOCYTES A "ONE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CCLLS A MORE "PLUMP' APPEARANC THIS 'EMLAROED* APPEARANCE, THE CELLS ARE OTHERWI THE- FO-LLO-WING- -TISS-UES- WE-RE - UNR-EMA-RKAB-LE - AT- MICRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN (01). COAGULATING GLO ICGI. LF. TESTIS ITEOI. LUNG ILUI. PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA I --------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: CS?655 SEX: MALE DOSE ONOUP: 4 SACRIFICE STATUS: SCHEDULED SECOND INTERIM $A( DATE Of DEATH: 02 $1 STUDY DAY OF DEAT14: S? STUDY WEEK Of DEAT14: 9 TERMINAL ADDY WEIGHT: 479. DATE AND TIME OF 4ccliopsy: 02(14/91 $:Sl PROSECTOR. NUT" SCHMITZ RECORDER: JILL BEVAN P-O-S-T--F-I-X--W-[-IO-N-E-R:---N-OT--A-V-A-IL-A-B--E----------------P-AT-H-O-LO-G-I-ST-:---D-R-.--T-OM--P-A-LM-E-R----------------W-E-IG-H-E-R:---V-A-L --QU-I-N-N--------------------- ABSOLUTE ORGAN WEIOHT O-R-O-A-N-N-A-M-E-------------- (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- OROAN TO BRAIN WEIOHT RATIO -------- 0 R0 S TA T ------- BRAIN (ON) 2.1033 .4316 % 1.0000 WEIGHT LIVER ILI) 23.6742 4.9373 % 11.2SS7 WEIGHT LUNG (LU) 1.6199 .3378 % .7702 WEIGHT LF. TES71S ITEO) 1.6010 .33S3 % .764S WEIOHT AT. TESTIS (TEII 1.5815 .3298 % .7sto WEIGHT ACCESS/SEX ORO ISO) 3.6047 .7sto % 1.7138 WEIGHT ------- - ------------------------------------------------------------------------------------------------ P A I " 0 L 0 0 Y- 0 8 2 f R V A 7 1 0 N S a & A OROAN NAME KEYWORDS. ;E*VER*ITYD.ISTRIBUTION OROSS FREE-TEXT COMMENTS / "ISTOPAT"OLOGIC Fll* ------------------------------------------------------------------------------------------------------------- GENERAL LIVER URETHRA COMMENT ILI) (URI GIOOCSI: . .. . MICRO: MICRO: -ALi iliS6[i/6lt6AiS'146Ri4*Ai' ' * *"* * . . . . . . .. .. . .. .. ... . .. .. .. . .. -MINIMAL -14ipiRift6p,HY* 'HE,PA,70*C[*LLU,L*AR'*C:'NAR,AC,IE*A,IZE,0,9A*,Y CENTRILODULATED PATTERN IN WHICH THE NEPATOCYTES A MORE 14OHMENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARANC T141S "ENLARGED" APPEARANCE. THE CELLS ARE OTHERW -iE@TiOi iXiMiNiD*. itiS6E'146T'PilEi[iT' .. .. .. . THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - BRAIN (OR), COAGULATING OLD ICO). LF. TESTIS (TEO). LUNG (LU), PROSTATE (PRI. SEMINAL VESICLE ISV) -------------------------------------------------------------------------------------------------------------------- tn APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COYOSS SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SAC DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK Of DEATH: 9 TERMINAL IODV WEIGHT: 465.4 DATE AND TIME OF NECROPSY; 02/14/91 7:35 PROSECTON: KEVIN BILLINGS RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. YOM PALMER WEIGHER: VAL QUINN --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIOHT O-R-OA-N---NA-M-E-------------- (GRAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 09 0 A STA T -------- BRAIN 1L641 2.0411 .4396% 1.0000 WEIGHT T LIVER 1 LUNG ILU) 21.443S 1.3188 4.60is % .2984 % 10.SOS9 .6804 WEIGHT I WEIGHT T LF. TESTIS ITEO) 1.9006 .4084 % .9312 WEIGHT I AT. TESTIS (TEII 1.9sos .4210 % .9600 WEIGHT T ACCESS/SEX ORO ISO) 2.6750 .5740 % 1.3106 WEIGHT I --------------------------------------------------------------------------------------------------------------------- ORGAN NAME ---------------------- KEYWORDS. ; EVERITY, DISTRIBUTION --------------------------------------- GROSS FREE-TEXT COMMEIOT; */* HISTOPAT"OLOGIC FIND ------------------------------------------------- GENERAL COMMENT LIVER ILI) GI(OICSI:-ALi iliS6E@/6lt6AiS'N6Ri4Ai . . . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -MINIMAL. FOCAL -MINIMAL -INFIL7RATES.140NOMUCLEAR CELL -HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATED PATTERN IN WHICH THE NEPATOCVTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED OIVIMG THE CELLS A MORE 'PLUMP' APPEARAMC THIS 'ENLARGED' APPEARANCE, THE CELLS ARE 07"ENWI - -TH-EF-OLL-OW-INT-GIS-SU-EWS-ERE-UN-RE-MA-RKA-BAL-TEM-ICR-OS-CO-PEI-XCAM-IN-AT-IO-N:- - - - - - - - - - - - - -------B--RAINJ-O-R-)-.-C-O-A-G--U-L-A-T-I-O-NL-GD--(-C-O-)-,-L-F-.-T--E-S-T-I-S-I-T-E-O-)--.L-U-N-G-I-L-U--)-.P-R--O-S-T-A-T-(-EP--R-)-.S-E--M-I-N-A-V-L--E---S--I--C---L--IE---S-V-)--.U-R-E-T--H-R-IA--- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANlt4AL 14UH13ER: C67657 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SAC DATE OF DEATH: SIWY DAY Of DEATH: 55 STUDY WEEK OF DEA714: I TERMINAL 6ODY WEIGHT- 469.6 DATE AND TIME OF0'4!C'@OoP'SY: 02 12/91 7:45 PROSECTOR: NANCY DIEDRICH RECORDER: DENNIS HOFFMAN POSY-FIX WEIGHER: NOT AVAILA13(E PATHOLOGIST: DR. 70H PAL14ER WEIGHER: PATHOLOGY DEPART --------------------------------------------------------------------------------------------------------------------- ORGAN NAME --------------------- ABSOLUTE OROAN WEIGHT (GRAMS) -------- ORGAN WEIGH7 RELATIVE TO BODY WEIGHT III -------- ORGAN 70 BRAIN WEIGHT RATIO -------- 0 R0 A S--T--A--T-- BRAIN ILBRli 2.1691 6 .4616 % 1.0000 WEIGHT T LIVER 21.9855 lo$4 % 13.3629 WEIGHT 7 LUNG ILU) 1.5997 .3404 % .7375 WEIGHT I LF. TESTIS (TEO) 2.0112 .4280 % .9272 WEIGHT 7 NT. TESTIS OITEIISO) 1.9089 .4002 % .$too WEIGHT T ACCESS/SEX 0 3.2183 .0977 % I.Sli& WEIGHT I ------------------------------------------------------------------------------------------------------------------- P A 7 0 0 L 0 0 V, 0 9 S f R V A 7 1 0 N S obA * ORGAN NAME KEYWORDS. ;EOVE6RITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND ------------------------------------------------------------------------------------------------------------- LIVER ILI) i4l@Rb:'-Alkli4AL.'F6CiL* -MINIMAL li#FiLiRi7E'S*.MO6I#6ON6UCL1EAAR *CiELLi * * * ' * ' ' * ' ' -"YPERTROPHY "EPATOCILLULAR: CHARACTERIZED BY AN CEN7RILOSULATED PATTERN IN WHICH THE HIPA70CVTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A 14DRE "PLUMP" APPEARANC THIS "ENLARGED" APPEARANCE. THE CELLS ARE OTHERWI MESENTERIC LM INS (IR4SS: -kOiT@E6 MICRO: -UNREMARKABLE HISTOPATHOLO(ITCAL COMMENT: -DARK iE6 iN6 iAi iC6LL'E6TiDi . ... .. )NO MICROSCOPIC CORRELATE TO GROSS OBSERVATION. THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN JOR). COAGULATING OLD (CGI. LF. TESTIS (TEO). LUNO ILU). PROSTATE IPRI. SEMINAL VESICLE ISV). URETHRA I --------------------------------------------------------------------------------------------------------------------- in c@ t.n INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7651 SEX: MALE DOSE ONOUP: 4 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE DATE OF DEATH: 03/26/91 STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TE NMI MAL 600Y WEIGHT: 444. DATE AND TIME OF NECROPSY: 03(26/91 7:59 PROSECTON: JULIE A. LARSEN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: DR. TOM PAL14EN WEIGHER: JOE M. KIZZIEE --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 pa A ORGAN NAME ---------------------- (GRAMSP -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- S TA T -------- BRAIN R 2.1339 .4805 % 1.0000 WEIGHT T LIVER JOLIJ 19.2486 4,3343 % 9.0204 WEIGHT I LUNG (LU) 1.5343 .3455 % .7100 WEIGHT I LF. TESTIS 0 1.7072 .3844 % .8000 WEIGHT 7 RT. TESTIS ITTEEII 1.6782 .3779 % .7864 WEIGHT T ------A-C-CE-S-S/-S-E-X ---0-0----S-o-l --------------3.-2-9-4-4-------------------.7-4-1-1--% --------------I-.S-4-3-8------------W-EI-G-HT---T---------------- ORGAN LIVER P A T N 0 L 0 (kV, 0 8 S E R V A T 1 0 N S fta k NAM-E -------------K-E-Y-WO-R-D-S,---;-E-O-VE-R-IT-Y-,--D-IS-T-R-IB-U-T-IO-N----------O--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-O-PA-T-H-OL-O-a-lC----FI-N-D------- (LI) . . . . . . . . . . . . . . I . . . . . . . . MICRO: -SLIGHT. FOCAL -SLIGHT -liFiLiNATiS*M6N6N6CiEAlt'CiLi -HYPEITTROPHY HEPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATto PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL @ORDERS ROUNDED GIVING THE CELLS A MORE "PLt)MP" APPEARANC PROSTATE THIS *ENLAROED" APPEARANCE. THE CELLS ARE OTHERWI (PRI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GROSS: -HASS(ES) -NIGHT SIDE: SINGLE MASS TAN AND FIRM. 10 MM IN D SECTION EXUDED GREEN CRIAMY SEMIFLUID; COLLECTED MICRO: -MODERATE, FOCAL REOIONAL LN (LUMBAR) NORMAL ICAP 61 -INFLAWATION. CHRONIC ACTIVE THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - BRAIN (SRI. COAGULATING ULD (C(ii. LF. TESTIS ITEOI. LUNG ILUI, SEMINAL VESICLE (SVI. URETHRA (URI --------------------------------------------------------------------------------------------------------- 4.n c@ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER:C97OSS SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SAC DATE Of DEATH: 01/24/91 STUDY DAY OF DEATH- 36 STUDY WEEK OF DEATH: 6 TERMINAL 600Y WEIGHT: 416. DAVE AND TIME OF NECROPSY: (24/91 1:07 OROSECTOR: JULIE A. LARSEN RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVATLAoOl E PATHOLOGIST: DO. TOM PAL04ER WEIGHER: DEBBIE PIRKEL ------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME (GRAMSI ---------------------- -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Ra STA T ------- BRAIN JON11 2.1717 .5237 % 1.0000 WEIGHT LIVER L 24.7761 s.esse % 11.3720 WEIOHT LUNG ILUI I.SS64 .3912 % .7603 WEIGHT LF. TE TI TEO I.SI26 .3636 % .6943 WEIGHT AT. TESTIS JTEIISO) 1.4907 .3s$3 % .6842 WEIGHT ACCESS/SEX 0 2.3142 .5563 % 1.0622 WEIGHT -------------------------------------------------------------------------------------------------------------------- ORGAN NAME KEYWORDS. ISEVAER*ITY. P ADISTTRHIB0UTILON0 0 Y- 0 6GRSOSfS R FVREEA-TtEXT1 0COHMMSENTSa a/ a HISTOPATHOLOGIC FIM -------------------------------------------------------------------------------------------------------- GENERAL COMMENT (1100SCI: -AL@ iliS6Ei/6ft4AiS*N6lti4AL LIVER (LI) . . . . . .. .. ... MICRO: -t@06EiAiE*l iO6A@ -SLIGHT C'O'AQ'UL*AT*IVE* "E"'O@HYAlr'EPA'OCELL'LA': CHARACTERIZED BY A CENTRILOGUL ED PATTERN IN WHICH THE HEPATOCYTES A 14ORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAM THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHERW THE- FO-LLO-WING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - - - BRAIN JOR). COAGULATING GLO ICGI. LF. TESTIS (TEOI. LUNG ILU). PROSTATE IPRI. SEMINAL VESICLE ISV), URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COTGOO SEX. MALE DOSE ONOUP: 4 SACRIFICE STATUS: SCHEDULED. FIRST POST-RECOV DATE OF DEATH: OS/17/91 STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: 557.8 DATE AND TIME OF NECROPSY: 05(17/91 8:10 PROSECTOlt: JULIE A. LARSEN RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILAO E PATHOLOOIST: DO. 1(*4 PALMER WEIGHER: DENNIS HOFFMAN --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME (GRAMSI ---------------------- -------- ORGAN WEXONT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0R0 A S TA T -------- BRAIN 1L00li 2.3756 42SO % 1.0000 WEIGHT T LIVER 20.04S$ 3:7013 % 8.6908 WEIGHT T LUNG (LUP 1.72s7 .3004 % .7264 WEIGHT T LF. TESTIS ITEO) 1.7347 .3110 % .7302 WEIGHT T RT. TESTIS OITEIISO, 1.7106 .3102 % .749S WEIGHT T ------A-C-CE-S-S/-S-E-X ----0--------------------3.-8-0-4-5--------------I---------.-0--8--2-1--%--------------1-.-6-0-i-s------------WE-I-GH-T---T------- ola 6 P A T " 0 L 0 0 Y 0 0 5 E ItV A T 1 0 N S 0 fth ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOOIC FIND -----------------------------------------------------7---GENERAL COMMENT ---------------------------.--.--7---.---.---.--.-.--.--- (1100CSI-:ALi iliS6Ei/6RdAiS*N6RAA@ ' ' * . . . * '* . *. *' '* * - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN 113PI. COAGULATING OLD ICGI, Lf. TESTIS (TEO). LIVER (Lll. LUNG ILU). PROSTATE (FRI. SEMINAL VESICLE (SV -------U-R-ET-H-R---I-U-R-)-------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANlt4AL PATHOLOCIY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- A141MAL NUMBER: C$7661 SEX-. 14ALE DOSE GROUP: 4 SACRIFICE STATUST SCHEDULED SECO14D INTERIM SAC DATE Of DEATH: 02/12/91 STUDY DAY OF DEATH: 55 STUDY WEEK OF DEATH: I TERMINAL 600Y WEIGHT: 412.9 DATE AND IIME OF NECROPSY: 02(12/91 9:22 PROSECTOlt: KEVIN BILLINGS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: -N-O-T--AV-A-I-LA--O --E----------------P-AT-H-O-LO-G-I-ST-:---0-4.---TO-M--P-A-LM-E-R----------------W-EI-G-H-ER-:---P-A-TH-O-L-OG-Y---D-EP-A-RT------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIOHT RELATIVE TO BODY WEIGHT (%I ORGAN TO BRAIN WEIGHT RATIO 0 R0 A S TA I ORGAN NAME - --------------- JORANSI -------- -------- -------- -------I- BRAIN 1L90li 2.1541 S211 % t.0000 WEIGHT LIVER 17.9125 4.* 3312 % 1.. 3iss WEIGHT IT LUNG ILU) 1.423S .3448 % 6608 WEIGHT I LF. TESTIIS ITEO) RI. TEST S TEI) 1.5288 I.S247 .3703 % .3693 % .7097 .7078 WEIGHT WEIGHT T ACCESS/SEX ORO ISOI 2.5689 .6222 % 1.1926 WEIGHT T --------------------------------------------------------------------------------------------------------------------- P A T N 0 L 0 0 V 0 8 S E P V A T 10 N S * * A OR-G-AN---NA-M-E-------------K-E-Y-WO-R-D-S,---;-E-*V-F-.-R*-I-TY-.-D-IS-T-R-IB-U-TI-O-N------------ GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC FIND ------------------------------------------------- GENERAL COMMENT LIVER ILI) GAGOCSI:-iLE iliS6Ei/61tdAiS'N6NkAL . . ... .. . . . . . . . . . . . . . . . . . .HiPiR'TR'OP''HHEY**PA'TOC'E'L'LC''UHLAAR*'*A'C:*TE'R*'IAZNE*D'B'Y CENTRTLOBULATEPDATTERNIN WHICHTHE NEPATOCYTES A MORE NOMOOENEOUS CYTOPLASM AND THE CELL BORDERS MOUNDED GIVING THE CELLS A MORE *PLTIMP" APPEARANC THIS 'ENLARGED" APPEARANCE, THE CELLS ARE OTHERWI THE- FO-LLO-WINO- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON-.- - - - - - - - - - - - - - - - BRAIN IBR). COAGULATING GLO ICGI. LF. TESTIS ITEO), LUNG ILUI. PROSTATE IPRI. SEMINAL VESICLE (SVI. URETHRA -------- ----- ------------------------------------------------------------------------------------------------ C2 40 INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COI@62 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE OF DEATH: 02413/91 STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: 431. DATE AND TIME OF CROPSY: 02 13/91 7:50 PROSECTON: RUTH SCHNITZ RECORDER: RUTH SCHMITZ POST-FIX WEIGHER: NOT AVAILAB(E PATHOLOOIST: ON. TOM PALMER WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME (GRAMS) ---------------------- -------- OROAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 AG S TA T ------- BRAIN JOLRIJ 2.0559 .4762 % 1.0000 WEIGHT LIVER 20.0101 4.0352 % 9.7330 WEIGHT LUNG ILU11 1.3732 .3181 % .6679 WEIGHT LF. TE T 1.5487 .3587 % .7s33 WEIGHT RT. TESTIS TE l.sll3 .3sol % .7351 WEIGHT ACCESS/SEX 017GEOIlsol 3.2091 .7435 % l.s6l3 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 Y 0 8 S E N V A T 1 0 N S A ftA O-R-GA-N--N-A-M-E --------------KE-Y-W-OR-D-S-. --;-E-AV-E-R-IT-Y-.--D-IS-T-R-IB-U-T-IO-N-------------G-R-O-SS---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---"I-S-T-OP-A-T-HO-L-O-GI-C---F-I-N------- LIVER ILI) ... .. MICRO: -AlilkAL' -HiPiltilt6p",Y, "'E'PA,TOC,[L,LU'LA,'RC*H:,AR*AC,TE,R,IZE,D,B,Y CENTRI LOBULATED PATTERN IN WHICH THE NEPATOCYTES A 14ORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAN THIS "ENLARGED' APPEARANCE, THE CELLS ARE OT"ERW 14ANDIO/CERV LM AR'OIS:--@AiGi . . . . . . . ... . . . . . . . . .PICAT: 6P'T6 i6'X*S*X'S'Pt@ iF6R'C6miAili0i iEiT* (CAP ii MICRO: -MODERATE. FOCAL -HYPERPLASIA. LYMPHORETICULAN CELL THE- FO-LLOW-ING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - BRAIN (SRI. COAGULATINO OLD (CGI. LF. TESTIS (TEO). LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA - -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67663 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SACR DATE OF DEATH: 01/23/21 STUDY DAY OF DEATH: 3S STUDY WEEK OF DEATH: S TERMINAL 6ODY WEIGHT: 413.9 DATE AND TIME OF NECROPSY: 01(23/91 7:57 PROSECTON: NANCY DIEDRICH RECORDER: JILL BEVAN POST-FIX WEI(IHER-. NOT AVAILAO E PAT14OLOGIST: DR. TOM PAL14ER WEIGHER: GAIL M. NOVAK -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OROAN NAME ---------------------- (GRANSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0P 0A S TA T -------- BRAIN ILBli 2.0214 4884 % 1.0000 WEIOHT T LIVER 2t.1019 S.0998 % 10.4422 WEIGHT T LUNO ILU) 1.4956 .3013 X .7399 WEIOHT T LF. TESTIS OITEOISOI 1.6313 .3941 % .8070 WEIGHT 7 R7. TESTIS TEI 1.0523 .3992 % .1174 WEIGHT T ACCESS/SEX (k 2.3713 S729 % 1.1731 WEIOHT T --------------------------------------------------------------------------------------------------------------------- a h a P A T H 0 L 0 0 Y - 0 B 9 E N V A T 10 N S A 6 * OROAN NAME KEYWORDS.SEVERITY.DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIND -------------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER ILI) QI'03CSI-:AL@ itiS6Ei/6ftdAiS'N6ltkA@ -MINIMAL . .. . .. .. .. . :IiFiLiltATES,HONONUCLEAR'CELL -HYPENTROPHY,,HEPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBUL ED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE 'PLtN4P* APPEARANC THIS "ENLARGED* APPEARANCE. THE CELLS ARE OTHERWI - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: -------G-R-AI-N--I-R-R-I-. -C-O-A-G-UL-A-T-I-NG---O-LD---IC-O-O-,--Lf-.--T-E-S-TI-S--I-T-E-O-I,--L-U-N-G--(-L-UI-,--P-R-OS-T-A-T-E--I-P-R-I,--S-E-MI-N-A-L--V-E-SI-C-L-E--(-S-V-I.--U-R-E-TH-R-A------------------ INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER-. C$7664 DATE OF DEATH: OS/10/91 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV STUDY DAY OF DEATH: 148 STUDY WEEK Of DEATH: 22 TERMINAL 6ODY WEIGHT: 094. DATE AND T114E Of NECROPSY: OS LS/91 7.34 PROSECTOR: GAIL M@ NOVAK RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER: JACK SERFORT ----------------------------------------------------------------------------------------------------------------- ORGAN NAME -------------------- ABSOLUTE ORGAN WEIGHT (GRAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0R a S TA T ------- BRAIN JLOlRi 2.1989 3167 X 1.0000 WEIGHT LIVER 20.9493 3.*0173 % 9.S2?2 WEIGHT LUNG ILU) 2.0241 .291S X .9205 WEIGHT LF. TESTIS E 1,0828 .2424 % .7053 WEIGHT RT. TESTIS ITTE011 1.6040 .244L % .7708 WEIGHT ACCESS/SEX 0 0 SO) 4.3942 .6329 % 1.9914 WEIGHT -------------------------------------------------------------------------------------------------------------------- PA T"0 L00 Y 0 0 SE P VA T 10N S 6h 4 ORGAN NAME KEYWORDS, ;['VIER*ITY.DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC FIN -----------------------------------------------------:-------- ------------------------------------------------ GENERAL COMMENT G@(OICSI:-ALL itiS6Et/bRdAkS'N6RM'A'L @ . : - - - @ .. . .. .. . LIVER (LIJ . . . . . . . * ' ' * ' * :IiFiLiRATiS'"6N6CiEAft'CiLi - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN IBRI. COAGULATIN(I GLO (CGI. LF. TESTIS (TEOI. LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SVI, URETHRA - --------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. p MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C6?66S SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA DATE OF DEATH: 01/23/91 STUDY DAY Of DEATH: 3S STUDY WEEK OF DEATH: S TERMINAL 600Y WEIGHT: 387 DATE AND TIME OF NECROPSY: 01 23/91 9:28 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. 70H PAL14ER WE10fiEN: GAIL M. NOVAK ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME (GRAMS) ---------------------- -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 R0 STA ------ BRAIN (DR) 2.0135 .5192 % 1.0000 WEIGHT LIVER ILI) 27.8119 7.1191 z 13.1475 WEIGHT LUNG LLU11 l.si5o .3907 % .7524 WEIGHT LF. TE 1.75as .4S29 U .1724 WEIGHT RT. TESTTIS ITTEE101 1.7807 .4807 % .8874 WEI(I"T ACCESS/SEX 0 0 SO) 2.4340 .6271 % 1.2091 WEIGHT ------------------------------------------------------------------------------------------------------------------- P A T N 0 L 0 0 Y- 0 8 S E P V A I 1 0 N S ib $I a ORGAN NA14E KEYWORDS. SAEV*ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC fl ------------------------------------------------------------------------------------------------------------ GENERAL C"ENT GI(OICSI-:AL@ itiS@Ei/6lt6AiS*N6tkAi LIVER (LI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -MINIMAL -HYPER7ROPHY 14EPA70CELLULAR: CHARACTERIZED BY CENTAILOBULATEO PATTERN IN WHICH THE NEPATOCYTE A MORE HOMOGENEOUS CY70PLASH AND THE CELL BORDE ROUNDED GIVINO THE CELLS A MAE *PLUMP" APPEARA THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHER GENERAL INFORMATION (XX) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GROSS: NECROPSY NOTE: )ANIMAL DIED PRIOR TO EXSANGUINATION - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN JOR). COAGULATING OLD (CO), LF. TESTIS (TEO). LUNG (LUI, PROSTATE IPR). SEMINAL VESICLE ISV), URETHRA ------------------------------------------------------------------------------------------------------------------- tn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA 14AZLEYON LABORATORIES AMERICA. INC. p MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67606 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST POST-RECO DATE OF DEATH: OS/17191 STUDY DAY OF DEATH: 149 STUDY WEEK Of DEATH: 22 TERMINAL 6()DY WEIGHT: 609 DATE AND TIME OF NECROPSVI: OS 17/91 7:46 PROSECTOR: JULIE A. LARSEN RECORDER: JOE N. KIZZIE POST-FIX WEIGHER: NOT AVAILAO(E PAT14OLOGIST: DR. TOM PALMER WEIGHER: JACK SERFORT ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 n (I ORGAN NAME (GRANSI TO BODY WEIGHT (%I WEIGHT RATIO STA ----------------------- -------- -------- -------- ------ BRAIN JLOINJ 2,2312 .3663 x 1.0000 WEIGHT LIVER 19.4sis 3.1945 % 1.7206 WEIGHT LUNG ILU) 1.6434 .2698 % .7360 WEIGHT LF. TESTIS ITEO) 1.4773 .242S % .6621 WEIGHT RT. TESTIS (TELI 1,460S .2398 % OS40 WEIGHT ACCESS/SEX ORO (SO) 4.0238 .6006 % 1.1034 WEIGHT ------------------------------------------------------I------------------------------------------------------------ P A T N 0 L0 0 V 0 0 S E P V A T 1 0 N S A * A ORGAN NAME KEYWORDS, SaEVaER*ITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC Fl --------------------- -------------------------------7 ------- ----------------------------------------------- GENERAL COMMENT GIGOCSI:-AL@ iliS6Ei/bft6AiS'N6RkAi ----- ------- -- --- --- --- --- --- -- --- ---- - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN OR) COAGULATING GLO (COI. LF. TESTIS (TEOI, LIVER (LIJ. LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE URETHRI IU*l ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7$97 SEX@ 14ALE DOSE GROUP-. 4 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE OF DEATH: 02/13/91 STUDY DAY OF DEATH: S6 STUDY WEEK OF DEATH: I TERMINAL &OOY WEIGHT: 494. DATE AND 7114E OF NECROPSY: 02 13/91 1:20 PROSECTOR: JULIE A. LARSEN RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILA13(E PATHOLOGIST: DR. TOM PALMER WEIDNER: JILL 13EVAN ------------------------------------------------------------------------------------------------------- ORGAN NAME -------------------- ABSOLUTE ORGAN WEIGHT JORAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 pa S TA T ------- GRAIN isit 2.1607 .4369% 1.0000 WEIGHT LIVER Lli 25.1940 S.2162 % 11.9378 WEIGHT LUNG ILU) 1.4690 .2971 % .6199 WEIGHT LF. TESTIS 017EIDIS01 1.0719 .3381 % .7738 WEIGHT IT. TESTIS TEI 1.7338 .3SOG % .8024 WEIGHT ACCESSISEX 0 3.S244 .7127 % 1.6311 WEIO"T ------- --------------------------------------------------------------------------------------------------- KEYWORDS. ; ORGAN MA14E EVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS HISTOPATHOLOGIC Ft - --------------------------------------------------- ------------------------------------------------ GENERAL COMWKT LIVER (LI) GiaoCst: -ALi tTiS6[i/61tdAiS'N61ti4AE MICRO: .-MlilkAi.*F6CAL' -MINIMAL -lifiAi*fATiOi.ditAN6L6MAT60ii -HYPENTROPHY NEPATOCELLULAR: CHARACTERIZED BY A CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTES A NNE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED OIVINQ THE CELLS A MORE 'PLUMP' APPEARA THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTHEOW THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNN-E14A-RKA-13LE- AT- MI-CROS-COP-IC - EX-A14I-NAT-ION:- - - - - - - BRAIN JOR). COAGULATING GLO ICG). LF. TESTIS ITEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE ISVI. URETHRA -------- -------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C6?668 DATE OF DEATH: 03/22/91 SEX: 14ALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC STUDY DAY OF DEATH: 03 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: 484. DATE AND TIME OF NECROPSY:,03(22/91 7:39 PROSECTOR: KEVIN BILLINGS RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAI AB E PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN MA14E ---------------------- (GRAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Ra STA t ------- BRAIN (BRI LIVER (LI) LUNG ILUI 2.03SI 14.2750 l.s2s$ .4197 % 2.9439 % .3147 % 1.0000 7.0144 .7497 WEIGHT Wlio"t WEIGHT LF. TESTIS JTEO 1.6947 .3495 % .8327 WEIGHT RY. TESTIS TEIISO) 1.8942 .3494 % .1325 WEIGHT ACCESS/SEX 0 2.9002 SB91 % 1.4290 WEIGHT ------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 Y 0 8 S E R V A T 1 0 N S ib& a ORGAN NAME KEYWORDS, ;EV*ERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC FIN ------------------------------------------------------------------------------------------------------------ KIDNEY(SI (KDI . . .. . . . . . . . . . GROSS: * -iAiGi OEiViSiEil . . . . . . . . . ; -BILATERAL MICRO: -PRESENT -DILATATION,PELVIC . . ... . . . . . . . . THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON--. - - - - - BRAIN JOR) COAGULATING GLO (CGI. LF. TESTIS ITEO). LIVER (LI). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (S URET (Uil -----------w ------------------------------------------------------------------w ------------------------------------- tn INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. p MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANTML NUMBER: Ctlgtt SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFI DATE OF DEATH: 03/22/91 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: 523 DATE AND TIME Of NECROPSY- 03 22/91 9:20 PROSECTON: RUT" SCHMITZ RECORDER: JOE tA. KIZZIE POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: ON. TOM PALMER WEIGHER: JILL BEVAN ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- JORAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Na S TA ------ BRAIN 8 2.06is .3934 % 1.0000 WEIGHT LIVER ILRIJ 24.0567 4.5954 % ll.o$95 WEIGHT LUNG ILU11 I.Sell .3021 % .1673 WEIGHT LF. TE T OITEO11 1.7125 .340S X .8647 WEIGHT 01. TESTIS TE 1.17ts .3397 % .8621 WEIGHT ACCESS/SEX 0 (SO) 3.6348 .6943 % 1.7631 WEIGHT ------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 V 0 8 S E N V A T 10 N S * & 0 ORGAN NAME KCYMOOS, ;E*VEANITV. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Ft -----------------------------------------------------7------- ----------------------------------------------- GENERAL COMMENT 04(OICSI-:AL@ itiS6Ei/6R6AiS*Ni)lti4A'L LIVER ILIJ .. ... MICRO: -AlilkAi . .. . .. .. . . .. . .. . .. . .. .. . ... . -HYPENTROP"Y NEPATOCELLULAR: CHARACTERIZED BY 0 CENTRILOSULATED PATTERN IN WHICH THE HEPATOCYTE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CTLLS A MORE -PLUMP- APPEARA THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHENT - -THE- F-OLLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - -- - - - - - - - - - BRAIN IBRI. COAGULATING GLO ICO). Lf. TESTIS ITEOI. LUNG ILU), PROSTATE (PA). SEMINAL VESICLE ISV). URETHRA ------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PRI MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CO?070 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE Of DEATH: OS/16/gi STUDY DAY OF DEATH: 141 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIOHT: 520.S DATE AND TIME OF NECROPSY: OS/18/91 8:38 PROSECTON: JULIE A. LARSEN RECORDER: JOE M. KIZZIEE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: 00. T014 PALMER WEIGHER: JACK SERFORT --------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 AG A ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT (1) -------- WEIO"T RATIO -------- S TA T -------- BRAIN ILO1N1 2.2129 .3506 % 1.0000 WEIOHT T LIVER 24.0443 3.$ISO % lo.sess WEIGHT I LUNO ILU) 1.7024 .2019 % .8100 WEIGHT T LF, TESTIS OITE?ISOI 1.9480 .3139 % .8803 WEIOHT 7 AT. TESTIS TE 1.9042 .3069 % .860S WEIGHT 7 ------A-C-CE-S-S-/S-E-X-----a-------------------4-.0-4-9-0-------------------S-s-z-s--%--------------1-.6-2-9-7------------W-E-IO-H-Y---I--------------- P A I"0 L0 6 Y,0 BS fPV AT 10N S &0 A O-R-OA-N---NA-M-E-------------K-E-Y-WO-R-D-S,---;-E-;E-*-R-IT-Y-.--DI-S-T-RI-B-U-T-IO-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-IC----FI-N-D------- GENERAL COMMENT GENERAL GIGOCSI:-iLi itiS6Ei/6R6AiS't46RkC . .. .. .. .. INFORMATION (XXI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GROSS: NECROPSY NOTE: @S14ALL NDVEABLE TISSUE MASS IVENTRAL HIND RIGHT) N CLINICAL OBSERVATIONS INADVERTENTLY NOT COMMENTE ABSENCE ON PRESENCE At TIME OF NECITOPSY THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - BRAIN BRI COAGULATING OLD ICO). LF. TESTIS (TEO), LIVER (LI). LUNG ILU). PROSTATE IPRI, SEMINAL VESICLE ISV URETHRI (Uil -------------- -------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. FRI --------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67671 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE DATE Of DEATH: 03425/91 STUDY DAY Of DEATH: 06 STUDY WEEK OF DEATH: 14 IERM114AL AODY WEIGHT: 550.6 DATE AND TIME OF ECROPSY: 03(25/91 9:49 PIIOSECTON: JACK SERFORT RECORDER: JOE M. KIZZIEE POST-FIX WEIGHER: NOT AVAILAB E PATHOLOCIIST: 00. T014 PAL04EN WEIGHER: STEVE VAN ADESTI --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO GRAIN 0 Na I ORGAN NAME --------------------- ((IRANS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- STA T -------- BRAIN (ON) 2.2293 .4047 % 1.0000 WEIGHT T LIVER (Lil 21.9610 S.2SOI % 12.9969 WEIGHT 7 LUNO (LU) 1.7119 .3122 % .7714 WEIGHT T LF. TE T ITTEEOII NT. TESSTIISS 1.9706 1.9121 .3S?O % .3512 % .1844 .$$so WEIGHT I WEIGHT T ------A-C-CE-S-S-/S-E-X---0--0---S-O-)--------------3-.-SO-I-I-------------------.-63-7-3--%--------------I-.-S?-4-6------------W-E-IG-H-T ---I--------------- P A T H 0 L 0 4 Y' 0 8 S E N V A T 1 0 N S iia a O-R-GA-N---NA-M-E-------------K-E-Y-WO-R-DS-.---;-E-*V-E-A-RI-T-Y,--DI-S-T-RI-B-U-TI-O-N----------G--R-O--S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-"-O-LO-G-I-C ---F-IN-D------- GENERAL COM14ENT LIVER ILI) GI(OICSI: -iLE iliS6Ei/6R6AiS'N6ltkAC .... . .. .. ... . .. .. .. .. .. . . MICRO: -MINIMAL. FOCAL -lifiLiftiTtS:M6N6N6CiEift*CiLi -SLIGHT -HYPERTROP"Y NEPATOCELLULAR: C14ARACTERIZED BY AN CENTRILOSULATED PATTERN III WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AMD THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAMC THIS "ENLARGED* APPEARANCE,. THE CELLS ARE OTHERWI THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - -------B-R-A-I-N--(-S-RI-,--C-O-AG-U-L-A-TI-N-G--O-L-D--I-C-GI-.--L-F-.--TE-S-T-I-S--I-TE-O-I-.--L-U-NG--I-L-U-)-. --PR-O-S-T-AT-E--I-P-R-)-,--SE-M-I-N-AL---VE-S-I-C-LE---I-SV-)-.--U-RE-T-H-R-A--I-------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. Pat M.A.DI.S.O.N....WI.S.CO.N.S.IN...U...S..A............-.-.-.-.-.-.-------------------------------------------------------------------------- ANIMAL NUMBER: CS?672 DATE OF DEATH: 03/2S/01 SEX: 14ALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFICE STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL 600Y WEIGHT: 4SO.S DATE AND TIME OF NECROPSY: 03/25/91 7:20 PROSECTON: JULIE A. LARSEN RECORDER: JOE N. KIZZIEE POST-FIX WEIGHER: NOT AVAILABLE PAT140LOQIST: DR. TOM PAL14EN WETQHER: JILL BEVAN ------------------------------------------------------------------------------------------------------------------- ORGAN NAME - --------------- ABSOLUTE ORGAN WEIGHT (GRAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%1 -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 AQ A S TA T -------- BRAIN ION) LIVER ILII LUNG ILU) LF. TESTIIS 1 2.1400 20.0120 1.4046 L.6706 .46sy % 4.3704 % .30si % .36SS % 1.0000 2.3141 .6s$4 .7140 WEIGHT T WEIGHT T WEIGHT I WEIGHT T RT. TEST S TE 1.6773 .3650 % .7838 WEIGHT T -------ACC-ES-S-/S-E-X---0-10-T-o-l-l-so-l--------------3-.3-3-49--------------------.-72-5-8--% --------------1-.5-5-9-4------------W-E-IG-H-T---T -------------- P A T H 0 L 0 0 Y@ 0 6 S C N V A T 1 0 N S Ita a ORGAN NAME KEYWRDS, ;E*V[*RITY.DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOOIC FIND ----------------------------------------------------------------------------------------------------------- GENERAL COMMENT c GA'03sl: -iL@ iliS6Ei/6R6AiS*N6ltkAC LIVER ILII ... .. .. .. .. .. .. .. .. . .. .. . . MICRO: -MINIMAL, MULTI-FOCAL -IkfiLiftATE'S'.M*ON'ON*UC'LEA*R*CE*L'L MINIMAL -HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED BY AN CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ROUNDED GIVING THE CELLS A MORE "PLU14P' APPEARANC THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OT"ENWI THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - -------B-RAIN IBRI. COAGULATING GLO ICGI. LF. TESTIS ITEOI, LUNG ILU). PROSTATE IPRI, SEMINAL VESICLE ISVI. URETHRA I -------------------------------------------------------------------------------------------------------- Ln INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON, WISCONSIN U.S,A. -------------------------------------------------------------------------------------------------------------------- ANtl4AL HUMBER@ CSIG?3 SEX: MALE DOSE OROUP@ 5 SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DATE OF DEATH: 03/26/11 STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: Sol DATE AND TimE or m[cropsy: o3 26/91 7:14 PROSECTON: JULIE A. LARSEN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: OR, TOM PALMER WEIGHER: JOE N. KIZZIEE ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT O-R-GA-N---NA-M-E -------------- (GRAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 Ra S IA ------ BRAIN BR 2.3361 .4$Sl % t.0000 WEIGHT LIVER ILII 23.3so3 4.GS33 % 9.9924 WEIGHT LUNG u l.so3a .2990 % .6434 WEIGHT LF. TEITLII (TEOI 1.7336 .34SS % .7419 WEIGHT RT. TESTIS (TEL L.IOIS .3405 % .731L WEIGHT ACCESS/SEX ORO (SO) 3.3836 .6143 % 1.4480 WEIGHT -------------------------------------------------------------------------------------------------------------------- .kOk11 P A T " 0 L 0 0 Y 0 0 S E A V A T 1 0 N S a * ik ORGAN MAKE KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC Fl -----------------------------------------------------@7------- ------------------------------------------------ GENERAL COMMENT (GCI GROSS: ALC fliS6Ei/6RdAi$'N6RkAC LIVER ILIJ MICRO: -iLiGAT' .. .. .. -"iPiltiNi)P''"HEY**PATOC'EiL6LCAhRA*i:A@TiltiZiD'Asi CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES A 14ORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARAN THIS 'ENLARGED' APPEARANCE. THE CELLS ARE OTHEft THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - BRAIN (ON), COAGULATING GLD (CGI. LF. TESTIS (TEO). LUNG (LUI, PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA -------------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 AH114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PRI MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- AN114AL IIUMEN: C$7674 DATE Of DEATH: 05/16/91 SEX: 14ALE STUDY DAY OF DEATH: DOSE GROUP: 5 148 STUDY WEEK SACRIFICE STATUS: OF DEATH: 22 SCHEDULED FIRST POST-RECOV TER14114AL 6ODY WEIGHT: OSS. DATE AND TIME OF NECROPSY: PROSECTON: JULIE A. LARSEN RECORDER: JOE M. KIZZIEE POST-FIX WEIGHER: N07 AVAILAOSS(EI-G-/9-1--I-:-S-l-------P-A-T-HO-L-O-GI-S-T-:---D-O-,--T-O-M--P-A-L-ME-R------------------W-E-IG-H-E-R:----JA-C-K--S-E-R-FO-R-T--------- ------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE OROAN TO BRAIN 0 aa ORGAN NAME JORANS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA I ------- ---------------------- BRAIN 1!11 2.2178 21.6330 .3324 % 3.3007 % 1.0000 9.7543 WEIGHT WEIGHT LIVER LUNG ILU) 1.6911 .2S$O % .762S WEI(IHT LF. TESST7I,SS t l,96s4 l@90$3 .2999 % .2912 % .8192 .1604 WEIGHT WEIGHT PT. IE ACCESS/SEX ITTE011 0 0 SO) 3.9142 .5972 % 1.7649 WEIGHT -------------------------------------------------------------------------------------------------------------------- *. A P A T H 0 L 0 0 Y* 0 8 S E N V A T 1 0 N S a * a ORGAN NAME K-E-Y-W-OR-D-S-,---;-EV-E-R-IT-Y-.---D-I-ST-R-I-B-UT-I-O-N-----------G-R-O-S-S --F-N-E-E--T-E-XT----C-OM-M-E-NT-S----/---H-I-ST-O-P-A-TH-O-L-OG-I-C----F-I-M--- -------------------- MANDIO/CERV LN IMN OROLS:'-@AiGi . . . MICRO: -MODERATE. MULTI-FOCAL --- -BiLiTiltiL: iA@H*ti 6P*Tb iS'X'16 i i 60@LiCi -HYPERPLASIA. LYMPHOftETICULAit CELL - ---- -- --- ---- --- --- - THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT MICROSCOPIC EXAMINATION: BRAIN,,11311,,COAGULATING OLD ICO ). LF. TESTIS ITEO), LIVER ILI). LUNG ILU), PROSTATE IPRI. SEMINAL VESICLE (S UNET u -------------------------------------------------------------------------------------------------------------------- tn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. p MADISON, WISCONSIN U S.A, ------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C6767S SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 36 STUDY WEEK OF DEATH: 6 TEITNINAL 6ODY WEIGHT: 401 DATE AND TIME OF MECROPSY: (24/91 7:19 PROSECTOR: DENNIS HOFFMAN RECORDER: VAL QUINN POST-FIX WEIG"Eitt NOT AVAtLAo6l E PAT14OLOGIST: DR. TOM PAL14ER WEIGHER: DEBBIE PIRKIL ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OftOAM WEIGHT RELATIVE ORGAN TO GRAIN 0 Na ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- S TA ------ BRAINit JOLNli LIVE 2,0458 29.9102 SO03 % 7.3161 x 1.0000 L4.6242 WEIGHT WEIGHT LUNG ILU) 1.441S .3s2s % .7040 WEIGHT LF, TESTIS ITEOI l.s379 .3761 % .?SIT WEIGHT NT. TESTIS ITEII I.Soll .3883 % .7701 WEIGHT ACCESS/SEX ORO (SOI 2.7sts .6729 % 1.34so WEIGHT ------------------------------------------------------------------------------------------------------------------- a A a P A T N 0 L 0 0 Y 0 8 S E R V A T 10 N S & * * ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC Fl ----------------------------------------------------------------------------------------------------------- GENERAL LIVER COMMENT GIGOCSI:-ALL iliS6Ei/61tdAkS'N6ltkA@ ILI) . . . . . MICRO: -AtilitAL . . . . . . -HiPiltilt6P'HY* H'E*PA*TOC'E*LL*ULA*R'*C:'HAK'AC*TE'N'IZE'D'BY* CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BOND[ ROUNDED GIVING THE CELLS A MORE 'PLUMP* APPEARA THIS "ENLARGED' APPEARANCE, THE CELLS ARE OTHER THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - -- - - - - - - --------BR-A-I-N--ION-)-.--CO-A-G-U-LA-T-I-N-G--G-LD---IC-G-I-.--L-F.--T-E-S-TI-S--I-T-E-O-)-. --LU-N-G--I-L-U-).--P-R-O-ST-A-T-E--(-P-R-I.--S-E-M-I-NA-L--V-E-S-I-CL-E--I-S-V-I-.-U-R-E-T-HR-A-------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABONA70RIES AMERICA. INC. p MADISON, WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------- AN114AL NUMBER: CO?076 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST POST-RECO DATE Or DEATH: OS/17/9t STUDY DAY Of DEATH: 149 STUDY WEEK OF DEATH: 22 TEfti4MlAL 6ODY WEIGHT: 399 DATE AND TIME OF NECROPSY: OS/17/91 1:37 PROSECTON: DEBBIE PIRKEL RECORDER: JOE N. KIZZIE POST-FIX WEIGHER: NOT AVAILABLE PAY14OLOCIST: DR. T014 PALMER WEIGHER: DENNIS NOFF14AN ------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0Aa ORGAN NAME (GRAMS) TO BODY WEIGHT WEIGHT RATIO S IA ---------------------- -------- -------- -------- ------ BRAINR JLORti 2.211207 .5541 % 1.0000 WEIGHT LIVE Is's 7 3.$IS4 % 7.1931 WIEIGHT LUNG ILU) 1.8120 .4036 % .72$S WE I(IHT LF. TESTIS -ITEO) 1 7404 .43S$ % .186S WEIGHT RT. TESTIS (TEI) 1.6794 .420S % .7sig WEIGHT -------AC-C-ES-S-/-SE-X---O-R-a--(-S-O-)--------------4-.5-6-7-4------------------1-.1-4-3-6--% --------------2-.0-5-4-0------------W-[-I-O"-L---------------- * 6 a P A T H 0 L 0 6 't 0 8 S E ftV A T 1 0 N S & 0.* O-R-GA-N---NA-M-E-------------K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-,---D-IS-T-R-IB-U-T-IO-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---"I-S-T-O-PA-T-"-OL-O-G-I-C ---Ft------- GENERAL COMMENT GIo(sICI: LUMBAR/ILIAC LN (JIL"I: .. . .. .. .. . PROSTATE (PRI oss -@Ai(li :BiLATiNAL': 6P'T6 iO*X* 10 X S MMICAP MICRO: -SLIGHT, MULTI-FOCAL -HYPERPLASIA. LYMPHOPETICULAN CELL . .. . . . . . . . . . . . . . . . . . . . . . ()ROSS: -MASS (C S I -* liV6L@IhG'EiTilti Olt6SiAiE: il'NGL*E'MA'S''S20'X' 2'0 TAN SOFT AND GELAT114OUS; SAME ON CUT tuRFACE; MICRO: -SEVERE, MULTI-FOCAL REGIONAL LN (SEE LUMBA -INFLAMMATION, CHRONIC fAl@TiVE THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - -------B-R-A-I-N--(OI -R)-.--C-OA-G-U-L-A-TI-N-G--O-L-D--(-C-O-).--L-F-.--TE-S-T-I-S--I-T-EO-)-.--L-I-VE-R--(-L-I-)-. -L-U-N-G--I-L-U)-.--S-E-MI-N-A-L--V-E-SI-C-L-E--(-S-V-I,--U-R-E-T-HR-A--(-U------------- cn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON, WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67677 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFIC DATE OF DEATH: OF03426/91 STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 532. DATE AND TIME ECROPSY: (20/91 9:04 PROSECTOR: DEBBIE PIRKEL RECORDER: JILL BEVAN POST-FIX WEIOHER: NOT AVAILA0O3 E PATHOLOGIST: DR. TOM PAL14ER WEIGHER: JOE M. KIZZIEE -------------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0Ra ORGAN NAME ------------------7--BRAIN JLOIRJ (GRAMSI -------- 2.1273 TO BODY WEIGHT -------.3099 % WEIGHT RATIO -------1.0000 S TA T ------WEIGHT LIVER 23.0049 4.3234 % 10.8141 WEIGHT LUNG (LU) 1.4376 .2702 % .67so WEIGHT LF. TTESTISS ITE 1.6225 .3049 % .7627 WEIGHT R T. ' TEI0I 1.0322 .3067 % .7673 WEIGHT ACCESS/SEX 0 Q Sol 2.6019 .4890 % 1.2231 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 Y- 0 8 S E 9 V A T 1 0 N S A * a ORGAN NAME KEYWORDS. ;E'VERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC Fl ------------------------------------------------------------------------------------------------------------- GENERAL LIVER COMMENT c Glaost: -ALi iliS6Ei/61tdAiS'N6RAAi . . . ... .. .. . ILI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -MINIMAL, FOCAL -MINIMAL -INFILTRATES.MONONUCLEAR CELL -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED BY A CENTRILOSULATED PATTERN IN WHICH THE NEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARA THIS 'ENLARGED* APPEARANCE. THE CELLS ARE OTHERW THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATI-ON:- - - - - - - - - - - - - - - -@) BRAIN (OR). COAGULATING GLD (CGI. LF. TESTIS (TEOI. LUNG (LU), PROSTATE (PRI. SEMINAL VESICLE (SVI, URETHRA -------------------------------------------------------------------------------------------------------------------- on t4 &n INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7671 SIX! MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED FIRST POST-RECOV DATE OF DEATH:005416/01 STUDY DAY OF DEATH: 148 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: SS2. DATE AND TIME F ECROPSY@ 05/16/91 1:59 PROSECTOlt: 019011 PIRKEL RECORDER: JOE M. KIZZIEE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: 09. TOM PALMER WEIGHER: JACK SERFORT ------------------------------------------------------------------------------------------------------------------- ORGAN NAME ---------------------- ABSOLUTE ORGAN WEIGHT (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 pa S TA I ------- BRAIN R 2,08940I @3737 % 1.0000 WEIGHT LIVER JOLI 1 19 4 S 3.S2$6 % 9.4415 WEIGHT LUNG (LU) 1,4101 .2552 % .0829 WEIGHT LF. TESSIISS 0 1.72is .3127 % .8366 WEIGHT RT. TE ITEEII 1.7204 .3114 Z .8332 WEIGHT ACCESSISEX 0 0 Sol 3.6050 .6520 % 1.7401 WEIGHT -------------------------------------------------------------------------------------------------------------------- P A T N 0 L 0 0 Y 0 0 S E ItV A I 1 0 N S 0 Ita ORGAN NAME KEYWORDS, ;EOVERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC FIN ----------------- --------------------------------------------------------------------------------------- GENERAL COMMENT G@GOCSI: -ALi iliS6Ei/61tdAAS NORMAL . . . * "'' ' ' ' * . . . . . . . . . * ' * . . . . . . . . . . - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - BRAIN JORI. COAGULATING OLD (CGI, LF. TESTIS (TEO). LIVER (LIJ, LUNG (LUI. PROSTATE IPRI. SEMINAL VESICLE (S URET"R (UP) -------------------------------------------------------------------------------------------------------------------- f.n r%3 4m INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: CG?679 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED TERMINAL SACRIFIC DI%IE Of DEATH-. 03/22/91 STUDY DAY Of DEATH: 93 STUDY WEEK Of DEATH: 14 TERMINAL 6ODY WEIGHT: 437. DATE AND TIME OF NECROPSY: (22/91 O:Dl PROSECTOR: OUT" SCHMITZ RECORDER: JOE H. KIZZIEE POST-FIX WEIGHER: NOT AVAIL238 E PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN ----------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 pa OROAN NAME -(-G-R-AM-S-O -- TO BODY WEIGHT ----- WEIGHT RATIO -------- S TA T ------- ------------------7--BRAIN IL1O1N 2.0361 AGSO % 1.0000 WEIGHT LIVER 29.2020 6.4476 % 13@8460 WEIGHT LUNG (LU) I.S302 .3519 % .7557 WEIGHT LF. TESTTS E 1.1sis .4233 % .9091 WEIGHT RT. TESTIS ITTE011 1.1670 .4040 % .8076 WEIGHT ACCESS/SEX 0 G SOI 3.OS$4 .8092 % l.sols WEIaNt -------------------------------------------------------------------------------------------------------------------- P A T H 0 L 0 0 V 0 0 S E 6 V A T 10 N S A I A O-R-GA-N---NA-M-E-------------K-E-Y-WO-R-DS-.---;-E-*V-E-*-NI-T-Y.--DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E---TE-X-T--C-O-M-ME-N-T-S ---/---HI-S-T-OP-A-T-HO-L-O-GI-C----Fl------ KIDNEY(S) (KD) Git6Si:*-@AiGi OE@ViSiEil* MICRO: -PRESENT LIVER (Lll . .. -ItiGAT': B'O*TH*C'OLL'EC'TE'D*. . . . * . . . . . . -DILATATION,PELVIC 'HE'PA'TO*CE*LL*UL'AR**:C*HAN*A'CTE*N'IZ'ED*B'Y*A CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARAN THIS *ENLARGED' APPEARANCE, THE CELLS ARE OTHERW THE- FO-LLO-WING- -TISS-UES- WE-RE -UMO-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - GRAIN IBRI, COAGULAYINO GLO ICGI. LF. TESTIS (TEO), LUNG ILU). PROSTATE (PRI, SEMINAL VESICLE (SVI, URETHRA ----------- ------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. PR MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C61610 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED SECOND INTERIM SA DATE Of DEATH: 02/12/91 STUDY DAY Of DEATH@ SS STUDY WEEK Of DEATH-. I TERMINAL 6ODY WEIGHT: 430 DATE AND TIME OF NECROPSY- 02/12/91 8!42 PROSECTOlt: NANCY DIEDRICH RECORDER: DENNIS HOFFMA PO$7-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: 00, TOM PALMER WEIGHER: PAT14OLOGY DEPAR ------------------------------------------------------------------------------------------------------------------- ORGAN NAME -------------------- ABSOLUTE ORGAN WEIGHT (GRAMS) -------- OR43AN WEIGNT RELATIVE TO BODY WEIGHT (1) -------- OftOAN TO BRAIN WEIGHT RATIO -------- 0 Ra S IA ------- BRAIN 184) 2.0122 .4670 % 1.0000 WEIGNT LIVER ILI) 30.3922 7.0532 % IS.1040 WEI(INT LUNG ILU) 1.5506 .3s$9 % .7700 WEIGHT LF. TESTIS JTEO ,ISO) I.SS$7 .3617 % .1746 WEIGHT RT. TESTIS TE ACCESS/SEX 0 l.s2l8 2.2374 .3s3l % .65$S % .7562 1.4101 WEIGHT WEIGHT --------------------------------------------------------------------------------------------------------- PA TN0 L0 0 V 0 9S EA VA I10 NS A 0* O-R-GA-N--N-A-M-E -------------K-E-Y-WO-R-D-S,---;-E-AV-E-R-IT-Y-.--DI-S-T-RI-B-U-TI-O-N----------O--R-O--S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-N-OL-O-C-II-C---Fl-------- GENERAL COMMENT GI(OICSI-:iLE iliS6Ei/6R6AiS'"i4Ai LIVER (LI) MICRO:.-iLi(i@T . . . . . . . . . . . . . . . . . -HYPERTROPiV. H.EP.ATO.CE.LL.ULA.R:. . -CH.AR.ACT.ER.IZ.ED-.BY CENTRILOBULATEID PATTERN IN WHICH T14E NEPATOCYTE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDER ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEARA THIS "ENLARGED" APPEARANCE. THE CELLS ARE OTHEM THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-A141-14A-TION-: - - - - - - - - - - - - - - BRAIN JOR). COAGULATING OLD (CO). LF. TESTIS ITEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA --------------------------------------------------------------------------------------------------------- 00 INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. p KADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------- ANIKAL NUMER: C$7681 SEX@ 14ALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST INTERIM SA DATE OF DEATH: 01/2S/91 STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: 372 DATE AND TIME OF NECROPSY: 01/2S/91 7:12 PROSECTOlt: JULIE A. LARSEN RECORDER: JACK SERFORT POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR, TOM PALMER WEIGHER: PATHOLOGY DEPA --------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- (GRANSI -------- ORGAN WEIG"T RELATIVE TO BODY WEIGHT (%I -------- OROAN TO BRAIN WEIGHT RATIO -------- 0p a S--T--A-- GRAIN ILSRli 2.0216 S431 % L.0000 WEIGHT LIVER 25.9118 6:0487 % 12.779S -WEIGHT LUNG ILU) 1.4so7 .3190 % Itss WEIGHT LF. TESTIS TE 1.6212 .4360 % .1030 WEIGHT RT. TESTIS TE? 1.6029 .4291 % .7005 WEIGHT ------A-C-C-E-SS-/-S-E-X --01-0---I-S-O-)--------------2,-7-1-51--------------------.7-2-8-1-%---------------1.-3-3-91-------------W-EI-G-HT----------------- aa 0 PA TH0 L0 0 Y 0 8 SER VA T10 NS *4 & OR-O-AN---NA-1-4E--------------KE-Y-W-OR-D-S-.--S-E-V-EN-t-l-Y.---D-I-ST-R-I-BU-T-I-O-N ----r------ G-R-O-SS---FR-E-E--T-E-X-T--C-O-MM-E-N-TS----/--H-I-S-TO-P-A-T-HO-L-O-(I-I-C--f-t------ GENERAL LIVER COMMENT OIGOCSI: -AL@ itiS6Ei/6gdAiS*H6Ri4Ai (LI) . i4l6R6:*-Alili4Ai,'M6Lil:FbCAL'* . -SLIGHT :IiFiLiRATis'm6N6"6ciEAR'CiLL -HYPERTROPHY "EPATOCELLULAR: CHARACTERIZED BY CENTRILOBULATED PATTERN IN WHICH THE NEPATOCYTE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL SORDE ROUNDED GIVING THE CELLS A MORE "PLUMP* APPEARA THIS "ENLARGED" APPEARANCE. THE CELLS ARE OTHER THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - - - BRAIN IBRI, COAGULATING OLD ICO), LF. TESTIS (TEO). LUNG ILU). PROSTATE IPRI. SEMINAL VESICLE (SVI. URETHRA -------- --------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMENTCA, INC. MADISON. WISCONSIN U S A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?612 SEX, MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERI DATE Of DEATH- 01/23/01 STUDY DAY OF DEATH! 3S STUDY WEEK OF DEATH: 5 TERMINAL BODY WEIGHT: DATE AND TIME Of NECROPSY: 01/23/91 7:11 PROSECTOR: NANCY DIEDRICH RECORDER: JILL SEVA POST-FIX WEIOHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: GAIL M. NO --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMSI TO BODY WEIGHT (%I WEIGHT RATIO s ---------------------- -------- -------- -------- BLRlAICNR 18LRli 2.14ss 57S2 % 1.0000 WE v 26.9262 7:2181 % 12.SSOI WE LUNG (LU) 1.3los @3691 % .0430 WE LF. TESTIS l@EO 1.5366 '4120 % .7162 WE RT. TESTIS TEIISO) 1,5994 .4280 % .74ss WE ACCESS/SEX OilO 2.83sy .7602 % 1.3217 WE --------------------------------------------------------------------------------------------------------------- ok 0 .4 P A T " 0 L 0 0 Y 0 8 S E N V A T 1 0 N S A h & ORGAN NAME KEYWORDS. SEVERITY, DISTRIBUTION GROSS FRELR-TEXT COMMENTS / HISTOPATHOLOGI -------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LIJ G@GOCSI: -ALi iliS6Ei/61tdAiS*N61ti4A@ . . . . . . * * ' ' ' ' * . . . . . . . . . .... . .. .. . .. .. .. . .. . MICRO: -SLIGHT -* HiPtftiR6P'"Y. ".EP.AT.OC.EL.L6LAit': "ChAiA6TiltiZiD CENTR ILOBULATED PATTERN IN WHICH THE NEPAT A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 8 ROUNDED GIVING THE CELLS A MORE 'PLUMP* APP THIS 'ENLARGED* APPEARANCE, THE CELLS ARE b THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - BRAIN JURI. COAGULATING OLD ICGI. Lf. TESTIS ITEOI, LUNG (LUI, PROSTATE (PRI. SEMINAL VESICLE ISVI. URE -------- ----------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7613 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST POST- DATE OF DEATH: OS/16/91 STUDY DAY OF DEATH: 141 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 05(16/91 8:02 PROSECTON: GAIL M. NOVAK RECORDER: JOE M. KI POST-FIX WEIGHER: NOT AVAILAID t PATHOLOGIST: DO. TOM PALMER WEIGHER: JACK SERFO -------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (GRANSI -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- s BRAINIt 18L1111 2,14S? 3618 % 1.0000 WE LIVE 17.4SIB 2.'9441 % 1.1306 WE LUF NG (LU) 1.5412 .2S99 % .7113 WE L . TESTIS 1110t I.S634 .2636 % .7216 WE RT. TESTIS 1.5733 .26S3 % .7332 WE ACCESS/SEX 0 0 ISO) 3.6301 .6137 % 1.6960 WE -------------------------------------------------------------------------------------------------------------- . a a 0 P A T " 0 L 0 0 V 0 8 S E A V A T 10 N S A * O-R-GA-N---NA-M-E-------------K--E-Y-WO-R-D-S,---S-E-VE-R-I-TY-.---OI-S-T-RI-B-U-T1-6-N----------G--R--O-S-S--F-AC-E---TE-X-T---CO-M-M-EN-T-S----/ *--H-I-ST-O-P-AT-H-O-LO-G-I------- GENERAL COMMENT LIVER (LIJ aloocst:-ALE iliS6lEi/6R6AkS'N6Ri4A@ .. .. .. . .. .. . .. .. .. . MICRO: -SLIGHT. FOCAL . .. . .. .. . .. .. .. .. . .. .. .. . -INFILTRATES,MONONUCLEAR CELL THE FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - -------B-R-A-I-N --(S-R-I-.-C-O-A-G-U-LA-T-I-N-G--O-L-D--(C-O-I-.--LF-.--T-E-S-TI-S--(-T-E-O-I.--L-U-N-G--(-L-UI-.--P-R-O-ST-A-T-E--(-P-R-I.--S-E-M-I-NA-L--V-E-SI-C-L-E--(-S-V--I--,---U--N--E-------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67614 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED FIRST POST- STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: DATE OF DEATH: OS/17/91 DATE AND TIME OF NECROPSY: 05 17/91 8:11 PROSECTON: DEBBIE PIRKEL RECORDER: JOE t4. KI P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-OT---AV-A-I-LA-O-(-E----------------P-AT-H-O-LO-G-I-ST-:---D-R-.--T-OM--P-A-L-ME-R----------------W-E-I-OH-E-R-: --D-E-NN-I-S--H-O-F--------------- ABSOLUTE ORGAN WETONT OROAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN MA14E (GRAMS) TO BODY WEIOHT 1%) WEIGHT RATIO s -------- -------- -------- ---------------------- 07AIRN (L071 2.0711 4219 % 1.0000 WE L VE 14.1733 2.887.2 % 6.8434 WE LUNG ILU) 1.4541 .2962 % .7021 WE LF. TE EO 1.65so .3371 % .7991 WE RY. TESTISS ITTEIISO, 1.7131 .3491 % .82?S WE --------A-C-C-E-S-S-/S-E-X----0--0-------------------------2-.-4-7-7-4-.-----------------------.-5-0-4-7--%------------------1-.-1-9-6-2------ WE 6A A PA TH0 L& 0 Y 0 8 SER VA T10 N S A& A O-R-G-A-N--N-AM-E-------------K--E-Y-W-O-R-D-S-.-S-E-V-E-R-I-TY-.--D-IS-T-R-I-B-U-T-I-O-N---------- OROSS FREE-IEXY COMMENTS / HISTOPAT"OLOGI ------------------------------------------- GENERAL COMMENT (iA'oscl:-ALL iltS6E@/bltdAAS'N6ltkA@ - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - At- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - IUlRtA 1,,CIIULATING OLD (CO). LF. TESTIS (TEO). LIVER (LI), LUNO (LU). PROSTATE IPR). SEMINAL VESIC ---------E-I-H"-RI-'-7-u------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANTHAL NUMBER: C$161S SEX-. t4ALE DOSE GROUP-. S SACRIFICE STATUS: UNSCHEDULED INI DATE OF DEATH: STUDY DAY OF DEATH: 21 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: NO DATE AND T114E OF0'4!C649O'PSY-. 01 l6/2L 13:30 PROSECTOR: DEBBIE PIRKEL RECORDER: DE6131E PIN POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOOIST: DR. TOM PALMER WEIGHER: NOT AVAILAO ---------------------------------------------------------------------------------------------------------------- 000 ANIMAL HAS NO ORGAN WEIGHTS RECORDED *40 ---------------------------------------------------------------------------------------------------------------- &a* PA TN0 L0 0 Y 00 S EA VA T 10 NS IA A ORGAN NAME KEYWROS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC ---------------------- ----------------------------------------------------------------------------------- LIVER (LIJ . ... .. .. . .. .. .. . .. .. . MICRO: -SLIGHT. FOCAL -MINIMAL SKIN ISK) . ... .. .. .. GROSS: -ABRASIONiSi/6L@EiAil6NiSi MICRO: -SEVERE. FOCAL -SEVERE. FOCAL -liFiLiRATiS'.M6Ni)1#6CiEAN*CiLi -HYPENTROPHY HEPATOCELLULAit: CHARACTERIZED CENTRILOSULATED PATTERN IN WHICH THE HEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 90 ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPE THIS 'ENLARGED' APPEARANCE. THE CELLS ARE 07 :@iLATE*RA'L* C'ER*VI*CA*L*NE'GIOM:'TW'O'TA*N*A*ND'RED 0 IS 25 X 16 MM AND THE OTHER IS IS X 10 MM (CO -DERNATITIS -ULCERATION THE- FO-LLOW-ING - -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON- - - BRAIN (BRI. COAGULATING OLD (COI, LF. TESTIS (TEOI. LUNG (LUI, PROSTATE (PRI, SEMINAL VESICLE (SVI. URET ---------------------------------------------------------------------------------------------------------------- Ln INDIVIDUAL APPENDIX 0 ANT14AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL 14UMBER: C$7586 SEX: t4ALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED TERMI14AL SAC DATE OF DEATH: 03/22/91 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TEITMINAL 600Y WEIGHT: N DATE AND TIME Of NECROPSY- 03/22/91 7:22 PROSECTOlt: KEVIN BILLINGS RECORDER,. JOE 14. KI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 OROAN NAME JORAMS) TO BODY WEIGHT WEIGHT RATIO s ---------------------- -------- -------- -------- BRAIN ILO1R1 2.1931 1.0000 EX LIVER 2S.9482 11.1317 EX LUNG ILU) 1.62S3 ------ .7411 EX LF. TESTIS (TEO) 1.8143 -------- .1592 EX al. TESTIS ITEI 1.2261 -------- .8330 EX ACCESS/SEX 000 ISO) 3.1012 -------- 1.4414 EX --------------------------------------------------------------------------------------------------------------- . & a A. P A T H 0 L 0 0 Y 0 8 S E N V A T 1 0 N S 0 0 ORGAN MAI* KFVWORDS, SEVEItIlY. DISTRIBUTION GROSS FREE-TEXT COMW"TS /a HISTOPAT"OLOGI --------------------- -------------------------------------------------------------------------------- GENERAL COMMENT LIVER ILI) GAOGCSI:-ALE iliS6lEi/6N6AiS'N6*0@@ kl6lt6:'-AlilkA@."F6CAL' -SLIGHT *INiFIiLTRiATAES1.MONO6NU6CLEAR1 A CEiLLi -HYPENT ROPHY HEPATOCELLULAR: CHARACTERIZED CENTITILODULATED PATTERN IN WHICH THE HEPATO A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 5 ROUNDED GIVING THE CELLS A MORE *PLUMP' APP THIS "ENLARGED* APPEARANCE. THE CELLS ARE 0 GENERAL INFORMATION IXXI OROSS: . . .. .. . .. tiiCiOiSi i#OiE: 118&i ki(147'IiA6ViRiE41@Y*N6l*TiKiN*Pil6R*T THEREFORE. ANIMAL WAS EXCLUDED FROM STATIST THE- FO-LLO-WING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - BRAIN JOR). COAOULATING OLD ICO), Lf. TESTIS ITEO), LUNG ILU). PROSTATE (PR). SEMINAL VESICLE ISVI. URE ------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL "UMBER: C$7687 SEX: MALE DOSE GROUP: S SACRIFICE S7A7US: SCHEDULED FINST INTERI DATE OF DEATH: 01/2S 91 STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NEC@OPSY: 01/2S/91 7;30 PROSECTON: JULIE A. LARSEN RECORDER: JACK SERF POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOOV --------------------------------------------------------------------------------------------------------------- ABSOLUTE OROAN WEIGHT ORGAN WEIGHT RELATIVE OROAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIOHT -------- WEIGHT NA710 -------- s BRAIN (OR) 2.2291 .5119 % 1.0000 WE LIVER (LI) 22.9772 5 0917 % 10.3078 WE LUNG ILU) 1,4440 :37 9 % .0470 WE L TEST S TEOI 1.5960 .4166 % .7160 WE NYF: TESTIS TEIISO, 1.7054 .4452 % .7$Sl WE ACCESS/SEX 010 2.4618 .8425 % 1.1043 WE --------------------------------------------------------------------------------------------------------------- 0 it4 P A T " 0 L 9)0 Y 0 8 S E P V A T 1 0 N S A & A ORGAN NAM-E-------------K-E-Y-W-OR-D-S-. --S-E-V-ER-I-T-Y,---D-IS-T-R-IB-U-T-IO-N----------G--R--O-S-S---F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---HI-S-T-O-PA-I-H-OL-O-G-I------- GENERAL COMMENT GIGOCSI:-ALL iliS6Ei/61tdAiS'N6RkAi . . . . . LIVER (LI) . .. . ... HYPE.NITt.ROP.HY. . H.EPATOCELLULAR: ChAiA6TiRiZiD CENT ILODULA71ED PA77ERN IN WHICH THE HEPA70 A "ONE HOMOGENEOUS CYTOPLASM AND THE CELL 0 ROUNDED GIVING THE CELLS A MORE 'PLUMP" APP THIS "ENLARGED" APPEARANCE. THE CELLS ARE 0 THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATION:- - - - - - - - - - - - - BRAIN JOR). COAGULATING OLD (CO), LF. TESTIS (TEO), LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE ISV). URE -------------------------------------------------------------------------------------------------------------- 4Ln ta tn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES . AMERICA. INC. MADISON. WISCONSIN U S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL MU14BER: C67008 DATE OF DEATH: 03/26/91 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED. TERMINAL SAC DATE AND TIME OF NECROPSY: STUDY 03/26/91 DAY OF 8:14 DEATH: 97 PROSECION I STUDY WEEK JULIE A. OF DEATH: LARSEN 14 TERMINAL BODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PATHOLOO S:T: ON. TOM PALMER RECORDER: JILL BEVA ---------------------------------------------------------------------------------WE-I-G-HE-R-:---JO-E--N-.--KI-Z--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS? ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ---------------------- -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- s BRAINR IBLRIJ L IVE 2.2203 S3$3 % 1.0000 WE LUNG 2S.7s4l 1.4103 6.2434 % 11.5994 WE LF. TE TI TEO11 l.s4lo .3419 % .3736 % .63S2 WE AT. TE 7is TE ACCESS/SEX 010 ISO) 1.6223 2.3141 .3933 % .6941 WE .7307 WE -------------------------------------------------------------S?-1-0--%--------------1-.-07-3-8------------W-E--------------- ORGAN NAME I oba a P A T " 0 L 0 0 Y 0 0 S E R V A T 1 0 N S * A a KEYWORDS, SEVERITY, DISTRIBUTION ----------------------------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOOT GENERAL COMMENT ------------------------------------------- LIVER ILI) GIGOCSI:-ALi iliS6Ei/61tdAiS'N6RkA@ GENERAL INFORMATION lxxl GROSS: . .. .. . .. . .. .. . . NECROPSY "OiE': -HYPERiR6PhY. hEiA*TOC'ELL'ULA'N*:'CH'AR*AC'TE'R'IZE*D' CENTRILOSULATED PATTERN IN WHICH THE "EPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 80 ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPE THIS "ENLARGED' APPEARANCE, THE CELLS ARE OT ),A*NI*MALD''IE*D'PR'I'OR'TO*E*X*SAN*GU'IN*A*TI'ONB'';L*OOD'S' THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - - -------B-R-A-I-N--(-ON-)-.--C-OA-G-U-L-AT-I-N-G--G-L-D--(-C-O)-.--L-F-.--TE-S-T-I-S--I-TE-O-)-.--L-UN-G--(-L-U-)-,-P-R-O-S-T-AT-E--I-P-R-)-.--SE-M-I-N-A-L--VE-S-I-C-L-E --(-SV-I-.--U-NC-T--------------- tn w cn INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LA130RATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67619 DATE OF DEATH: 01/23/91 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED FIRST INTER STUDY DAY OF DEATH: 3S DATE AND TIME OF NECROPSY: 01/23/91 9:32 STUDY WEEK OF DEATH: 5 TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: JULIE A. LARSEN PATHOLOGIST: OR. TOM PALMER RECORDER: JILL BEV ------------------------------------------------------------------------------W-E-IG-H-E-R-: --(-IA-I-L-M-.--N-------------- ORGAN NAME ABSOLUTE ORGAN WIEIG"T (ONAMSP ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN ---------------------- -------- -------- WEIGHT RATIO -------- s BRAIN LIVER ILBRII 2.00110 .5101 % 1.0000 WE LUNG (LU) 27.493 1.4362 7.0064 % .3860 % 13.7341 WE LF. TESTIS TE PT. TESTIS TEI0 1.4394 1.4378 .3680 X .1175 WE .7191 WE ACCIESSISEX io isol 2.2388 .3664 % S?OS % .7113 WE -------------------------------------------------------------------------------1-.-1-18-4-------------WE--------------- ORGAN NAME ------ ---------------- KEYWROS, a a* PA T N0 L0 0 Y SEVERITY. DISTRIBUTION 0 8 S E R V A t 1 0 N S a ika GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI ------------------------------ ------------------------------------- GENERAL COMMENT JGCI: _,L, I,S61E, . . . . . . . . ... . . . . . os S/ORGANS NOR14AL LIVER (LI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -MINIMAL, FOCAL -INFILTITATES,MDNONUCLEAR CELL -MINIMAL -HYPENTROPHY HEPATOCELLULAR: CHARACTERIZED CENT,T ILOBULATED PATTERN IN WHICH THE "EPAT A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 0 ROUNDED GIVING THE CELLS A MORE 'PLUMP- APP GENERAL INFORMATION (XX) GROSS. .. . .. .. .. . .. . .. . THIS *ENLARGED* APPEARANCE. THE CELLS ARE 0 NECROPSY NOIE,: ),,AN,I,MAD,'LIE,O*PR,10*R*T,EO*XiAiG6liAil6N -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - BRAIN (ON). COAGULATING OLD (CG). LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE (SVI, URE -------------------------------------------------------------------------------------------------------------- 4Ln w INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U,S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7890 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST INTENT DATE OF DEATH: 01/23/91 STUDY DAY OF DEATH; 35 STUDY WEEK OF DEATH: S TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECIOPSY: 01(23/91 7:44 POST-FIX WEIGHER: NO AVAILAB E PROSECTOR: JULIE A. LARSEN RECORDER: JILL SEVA ---------------------------------------------------------P-A-T-H-O-L-O-O-T-S-T-:----D-R-.---T-O-M---P-A-L-M-E-R----------------------W-E-IGHER: GAIL M. NO ORGAN NAME ABSOLUTE (GORRAGMASN) WEIGHT ORTGOANBODWYEIGWHETIGHRTELAT1I%V)E ORWGEAINGHTTO RABTRIAOIN 0 ---------------------- -------- -------- -------- s BRAT LIVER 1 1.95734 2s. 643 .5404 % 1.0000 WE LUNG (LU) LF TESTIS TEOI 1.4011 l.s479 7.0001 % .3837 % .4231 % 12.9544 WE .7100 WE .7144 WE RT: TESTIS if ACCESS/SEX 010 ISO) l.ssso 2.4ssl .4260 % .6723 7L .7083 WE 1.2441 WE --------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0 0 Y 0 8 S E R V A T 1 0 N S fth 4 KEYWORDS, *SEV6ER*ITY, DISTRIBUTION ---------------------------------------------------------G-R-OS-S FREE-TEXT COMMENTS / HISTOPATHOLOGI GENERAL COMMENT ------------------------------------------- LIVER (LI) GIOOCSI: -ALE iliS6Ei/6lt6AiS*N6Ri4A'L MICRO: -SLIGHT. -SLIGHT MULTI-FOCAL . . . . . . . . . . . -.IN.FI.LTR.AT.ES.,M.MMU.CL.EA.R . . CELL. . . . . . . . -HYPE:TROPHY HEPATOCELLULAR: CHARACTERIZED CENT ILOBULATED PATTERN IN WHICH THE HEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL OC ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPE THIS "ENLARGED* APPEARANCE, THE CELLS ARE OT THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - BRAIN JOR). COAGULATING OLD (CO). LF. TESTIS ITIO). LUNG (LU). PROSTATE (PR). SEMINAL VES-ICL-E -ISVI-, -URET- --------------------------------------------------------------------------------------------------------------- tn w co INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: COTOOL DATE OF DEATH: 03/25/91 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED TERMINAL SACR STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL IODY WEIGHT: DATE AND TIME OF NECROPSY: 03 2S/01 7:39 PROSECTOR: JACK SERFORT RECORDER: JOE N. KII POSI-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER: STEVE VAN A --------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT IGRAMS I ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN WEIGHT RATIO 0 ST ---------------------- -------- -------- -------- --- BRAIN LIVER ILOINI 1.903S .4911 % 1.0000 WEI LUNG ILUI 19.6471 1.2919 4.8406 % .3290 % 9.$SSO WEI SS16 WEI LF TESTIS TE? RT: TESTIS TE 1.5029 1.4089 .3703 % .3471 X .7539 WEI .7067 ACCESS/SEX 010 ISO) ------- 2.$SS7 .7100 % WEI 1.4478 WEI -------------------------------------------------------------------------------------------------- ORGAN NAME oba A P A T N 0 L 0 0 T 0 8 S E R V A T 1 0 N S a NJa KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC ------------------------------------------------------------------------------------------------------ GENERAL COMMENT c LIVER (LI) (il'.OS.I-A:.Li. i.li.S6E.i/.6R.6Ai.S'.N6.Ri4.Ai. . . . . . . . . . . . . . . . . . . . . . . . . . N16RO:-SLIGHT -HYPENTROPHY HEPATOCELLULAR: ChAiA6TiRiZiD* CENTRILOBULATED PATTERN I" W141CH THE NEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 80 ROUNDED GIVING THE CELLS A MORE *PLUMP" APPE THIS "ENLARGED' APPEARANCE, THE CELLS ARE 01 THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - -------BRAIN--(-S-R-I-,-C-O-A-G-U-LA-T-I-N-G--O-LD---(C-(-l-)-.-LF-.--T-E-S-TI-S--(-T-E-O-).--L-U-N-G--I-LU-)-.--P-R-OS-T-A-T-E--(-P-R-I,--S-E-MI-N-A-L--V-E-SI-C-L-E--I-S-V-I-. -U-R-E-T------------- iLn w to INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON MADISON, LABORATORIES AMERICA. WISCONSIN U.S.A. INC. -------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67692 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERI DATE Of DEATH: 01/24/91 STUDY DAY OF DEATH; 36 STUDY WEEK Of DEATH: 6 DATE AND TIME OF NECROPSY: 01/24/91 9:20 PROSECTOR: DENNIS HOFFMAN TERMINAL BODY WEIGHT: RECORDER: VAL QUINO POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: DEBBIE PIR --------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN c ---------------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- s BRAINR LIVE (OR) ILZI 2.0512 26.0918 .5247 % 1.0000 WE LUNG (LUI 1.6213 6.6741 % .A 1'48 % 12.7203 WE .7904 WE LF. TESTI RT. TESTIS ITTEE011 1.7223 .4406 % .1397 WE ACCESS/SEX 010 (SO) 1.7144 2.8217 .4316 % .1358 WE --------------------------------- .7218 % 1.37S$ WE 7 ----------------------------------------------------------------------------- a a a P A T H 0 L 0 (1 Y 0 0 S E R V A T 1 0 N S 6 0 * ORGAN NAME ---------------------- K-E-Y-WO-R-D-S.----S-EV-E-R-IT-Y-.---D-I-ST-R-I-B-UT-I-O-N -----------GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGI ------------------------------------------- GENERAL COMMENT LIVER ILI) GAGOCSI.: .-A.Li. i.li.S6.Ei/.6R6AiS*.N6.RI@.A@. . . . . . . . . . . . . . . . . . . . .. . .. . ..*. *. .* *. '. *. 141@ItO: -MINIMAL, FOCAL -MINIMAL -INFILTRATES,MONONUCLEAR CELL -HYPE:TTOPHY NEPATOCELLULAR: CHARACTERIZED CENT I OBULATED PATTERN IN WHICH THE HEPATO A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 8 ROUNDED GIVING THE CELLS A MORE 'PLUMP- APP THIS "ENLARGED" APPEARANCE, THE CELLS ARE 0 THE FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - BRAIN IBRI. COAGULATING GLD ICO). LF. TESTIS (TEO). LUNG ILU). PROSTATE IPRI. SEMINAL VESICLE (SVI, URE --------------------------------------------------------------------------------------------------------------- C3 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES .AMERICA. INC. MADISON. WISCONSIN U S.A. --------------------------------------------------------------------------------------------------------------- ANIMALNUMBER:COYB$3 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED FIRST INTERI DATE OF DEATH: 01124191 STUDY DAY OF DEATH: 36 DATE AND TIME OF NECROPSY: 01/24/91 8:44 STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: DENNIS HOFFMAN RECORDER: VAL QUINN ------------------------------------------P-A-TH-O-L-OC-I-TS-T-:--D-R-.--T-O-M --PA-L-M-ER-----------------WE-I-G-HE-R-:--D-E-B-BI-E---PI-N--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIO"T ORGAN TO BRAIN WEIGHT RATIO 0 ---------------------- -------- -------- -------- s BRAINR 1L0!l L IVE 2.0524 22.4973 -S105 % 1.0000 WE LUNG ILUI LF. TE TI OITIE[OJISOI 1.432S 1.5361 I.SI14 5.5963 % .3S$3 % .3121 % 10.9$IS WE .6980 WE .7414 WE RT. TESTIS ACCESS/SEX 0 3.1217 .3700 % .7713 % .7364 WE I.S244 -----------------------------------------------------------------------------------------------W-E--------------- ORGAN NAME a lb@% P A T N 0 L 0 0 Y 0 0 S E N V A T 1 0 N S * a a KEYWORDS. SEVERITY. DISTRIBUTION G-E-NE-R-A-L --C-O-MM-E-N-T -----c-----------------------------------------G--R--O--S-S --F-RE-E---TE-X-T---CO-M-M-EN-T-S---/---H-IS-T-O-PA-T-"-O-LO-G-I------- 04(olsl:-AL@ iliS6Ei/6R6AiS*t#64AA@ LIVER ILI) . . '-H*YPE*:'TRO'P*HY'*HE'PA'TOC'EL'L*ULA'R':C'"AR'AC'TE'R*IZE'D CENT ILOBULATED PATTERN IN WHICH THE HEPAT04 A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 8 ROUNDED GIVING THE CELLS A MORE "PLUMP" APP THIS "ENLARGED" APPEARANCE. THE CELLS ARE 0 THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - E -XAMI-NATI-ON:- - - - - - - - - - - - - ------B-R-A-I-N--(-BR-I-.--C-OA-G-U-L-AT-I-N-G--O-L-D--I-CG-)-.--L-F-.-T-E-S-T-I-S --(T-E-O-)---LU-N-O--(-LU), PROSTATE (PRI. SEMINAL VESICLE (SV), URE ---------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. MADISON. WISCONSIN U.S.A. INC. --------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: CO?694 SEX: MALE DOSE (IROUP: 5 SACRIFICE STATUS: SCHEDULED FIRST POST- DATE OF DEATH: OS/16/91 STUDY DAY OF DEATH: 148 STUDY WEEK OF DEATH: 22 TERMINAL 600Y WEIGHT: DATE AND TIME OF NECROPSY: 05/10/91 9:09 PROSECTON: GAIL M. NOVAK RECORDER: JOE N. KI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: JACK SERFC --------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT 04(IAN WEIGHT RELATIVE ORGAN TO BRAIN c (GRAMS) TO BODY WEIGHT (%I WEIGHT RATIO ---------------------- -------- -------- -------- s BRAIN JLONIJ 2.276831 @3125 % 1.0000 WE LIVER 20.02 3.3618 % 1.7881 WE LUNG LLUI 1.7147 .2979 % .7718 WE LF. TE TIS TEO 1.8411 .3091 % .8079 WE RT. TESTIS IIEIISO) 1.7286 .2902 % .7586 WE ACCESS/SEX 0 G 4.1212 .6918 % l.loss WE --------------------------------------------------------------------------------------------------------------- A A a P A T N 0 L-0 0 Y 0 8 S E 9 V A T 1 0 N S A A A ORGAN NAME ---------------------- KEYWORDS. SEVERITY, DISTRIBUTION GROSS --------------------------------------- FREE-TEXT COMMENTS / "ISTOPATHOLOGI ------------------------------------------- GENERAL COMMENT LIVER (LI) GA(OICSI:-ALE iliS6Ei/i)R6AiS'NbltkAi .. . .. ... .. .. .. . .. .. . .. .. .. . .. .. . .. .. ... . . .. .. .. MICRO: -MINIMAL. MULTI-FOCAL -INFILTRATES.MDNONUCLEAR CELL THE- FO-LLOW-ING - T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATIO-N: - - - - - - - - BRAIN JOR). COAGULATINO OLD (COI, LF. TESTIS (TEO). LUNG ILUI. PROSTATE (PRI. SEMINAL VESICLE (SVI. URE --------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67695 DATE OF DEATH: 03/22/91 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL SAC DATE AND TIME OF NECROPSY: 03/22/91 7;27 PROSECTOR: RUTH SCHMITZ TERMINAL 6ODY WEIGHT: RECORDER: JOE N. KI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PAL14ER WEIGHER: JILL BEVAN -------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIOHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 JORAMSI TO BODY WEIGHT WEIGHT RATIO s ---------------------BRAIN -------- -------- -------- I R 1L8Rli L VE LUNO ILU) 2.1189 21.7197 1.3370 S491 % 5.6465 % .3465 % 1.0000 WE 10.2835 WE .6310 WE LF TESTIS TEOI ItT: TESTIS TEI 1.1195 1.72SO .4715 % .4470 % .8s$7 WE ACCESS/SEX 010 ISO) ------- 3.0488 .7900 % .1141 WE 1.4319 WE ------------------------------------------------------------------------------------------------- ORGAN NAME a * 01 P A T H 0 L 0 0 Y 0 8 S E A V A T 1 0 N S h 4 & KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT CONKUTS / HISTOPATHOLOGI ------------------------------------------------------------------------------------------------------ GENERAL COMMENT c LIVER ILI) 134(o.lst.:-A.LE. i.li.S6.Ei./6.R6A.kS.*N.6l.ti4.A@. . . . . . . . . . . .. . .. .. . . . . . . . . . . . . . . . . MI@RO: -SLIGHT -HYPE TROPHY HEPATOCELLULAR: CHARACTERIZED CENTORILOSULATED PATTERN IN WHICH THE HIPATOC A NOR[ HOMOGENEOUS CYTOPLASM AND THE CELL OC ROUNDED GIVING THE CELLS A MORE *PLtfMP' APPE THIS 'ENLARGED' APPEARANCE, THE CELLS ARE OT - -THE- FO-LLOW-ING- -TISS-UES -WE-RE -UNR-EMAR-KAB-LE - AT- 14-1CRO-SCO-PIC - EX-AMIN-ATI-ON:- - - - - - - - - - - - - - - ------B-R-A-IN---(O-R-)-,-C-O-A-G-U-LA-T-I-N-G--O-LD---(C-O-I-.--L-F.--T-E-S-T-IS--(-T-E-O-)-,-L-U-N-G--(-L-U-).--P-R-O-ST-A-T-E--I-P-R-I-, -S-E-M-IN-A-L--V-E-S-IC-L-E--(-S-V-)-.--UR-E-T--------------- 4Pb L-P APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ------------------------------------------------------------------------------------------------------------------ ANIMAL NUMBER: C$7096 DATE Of DEATH: 01/24/91 SEX: 14ALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST INTERIM DATE AND TIME OF NECROPSY: STUDY DAY OF DEATH: 36 01 24/91 7:39 PROSECTON: STUDY WEEK OF DEATH: DENNIS 14OFF14AN 6 TERMINAL 6ODY WEIGHT: 3 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TON PALMER RECORDER: VAL QUINN --------------------------------------------------------------------------------W-E-IO-H-E-R:---D-E-BB-I-E--P-I-R-KE---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMSI ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0R ---------------------BRAIN (ON) -------1.8940 TO BODY WEIGHT (%I -------S404 % WEIGHT RATIO -------1.0000 ST ---WEIG LIVER ILI) LUNG (LU) 24.8083 1.2521 7.0700 x .3573 % 13.0942 WEIG .6612 LF. TESTIS AT. TESTIS T 011 1.0894 1.5699 .3107 % WEIG .5750 WEIG ACCESS/SEX 0 (1 SO) 2.3663 .4472 % .6749 % .1216 WEIG 1.2490 WEIG ----------------------------------------------------------------------------------------------------------------- ORGAN NAME P A T H 0 L 0.0 Y 0 0 S E R V A T 1 0 N S & A A KEYWORDS.' *SEV*ERmITY. DISTRIBUTION K-I-D-N-EY-(-S-)---I-K-D-I---.--.--.---.-I--.--.--.-.--.--.--.-.--.--.--.-.--.--.-.--.--.--.--.G--R-.-O-S-.-S -.-F-R.E-E-.--T.E-X-T.--.-CO-.M-E-NT-S----/---H-IS-T-O-PA-T-H-O-LO--G--I-C----- GROSS: -LARGE PELVIS(ES) MICRO: -PRESENT -BILATERAL: BOTH COLiE6TiD' Lf. TESTIS (TEO -DILATATION.PELVIC LIVER (LI) @ROSi:'-@OiTAiis'FiUiD' MICRO: -MODERATE. DIFFUSE ... .. .. .. . MICRO: -MODERATE. @OdA@ -MINIMAL LEFT: CLEAR FLUID -ATROPHY/DEGEHERATION -NECROSIS COAGULATIVI -HYPERTROANY HEPATOCELLULAR: CHARACTERIZED 0 CENTRI LOBULATED PATTERN IN WHICH THE HEPATOCY A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BOA ROUNDED GIVING THE CELLS A MORE "PLUMP* APPEA - - - - - - - - - - - - - - - - - - - - - - TH-IS -"EN-LAR-GED"- A-PPE-ARAN-CE.- T-HE -CEL-LS -ARE- OT-H en 4P41. INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7696 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED FIRST INTERI DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 36 STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 01/24/91 7:39 PROSECTON: DENNIS HOFFMAN RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: OR. TOM PALMER WEIGHER: DEBBIE PIR --------------------------------------------------------------------------------------------------------------- THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BPI. COAGULATING GLD (CGI. LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SVI. URETHRA (URI --------------------------------------------------------------------------------------------------------------- en INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67097 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED. TERMINAL SACR DATE OF DEATH: 03/20/91 STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 DATE AND TIME OF NECROPSY: 03 26/91 7:40 PROSECTON: JULIE A. LARSEN TERMINAL BODY WEIGHT: RECORDER: JILL 13EVAN P-O-S-T--F-I-X--W-EI-G-H-ER-:---F-OO-T-A-V-A-IL-A-O-(E-----------------P-A-TH-O-L-OG-I-S-T:---O-f-t-. -T-OM--P-A-L-ME-R----------------W-E-I-GHER: JOE N. KIZZ ---------------------------- ORGAN NAME ABSOLUTE (GORRAGMASNI WEIGHT ORTGOANBODWYEIGWHETIGHRTELA1T%I)VE OWREGIAGNHT TO RABTRIAOIN S0 T ---------------------- -------- -------- -------- --- BRAIN 1841 LIVER ILI) 2.1143 30.4173 .4389 % 1.0000 WEI LUNO JLU) LF TESTIS ITTEE011 1.4679 I.SISO .1220 6.3146 % .3047 % .3770 % 14.31$S WEI .0943 WEI .$see WEI AT: TESTIS ACCESS/SEX 0 0 Sol 1 4.1308 .3712 % ISYS % .1618 WEI I.OS37 WEI ---------------------------------------------------------------------------------------------------------------- OROAN NAME a a a P A T N 0 L 8.0 Y KEYWORDS. SEVERITY. DISTRIBUTI N ---------------------- 0 0 S E A V A T 10 N S a a * GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC ----------------------------------------------------------------------------------- GENERALCOMMENT ,loci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . OSS: -ALL TISSUES/ORGANS NORMAL LIVER (LI) . . SLIGHT -HYPERTROPHY NEPATOCELLULAR: CHARACTERIZED CENTRI LOBULATEO PATTERN IN WHICH THE NEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BOR ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEO THIS "ENLARGED" APPEARANCE, THE CELLS ARE OT THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - -- --- -------B-R-A-I-N --(-SR-I-,--CO-A-G-U-L-AT-I-N-G--O-L-D--f-CO-)-.--L-F-.--TE-S-T-I-S--(-TE-O-)-.--L-UN-G--I-L-U-)-.-P-R-O-S-TA-T-E--I-P-R-I-.-S-E-M-I-N-AL--V-E-S-I-CL-E---IS-V-). URETI -------------------- ab at APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7621 SEX: 14ALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST INTERI DATE OF DEATH: DI/25/91 STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 TERMINAL ADDY WEIGHT: DATE AND TIME OF NECROPSY: (25/91 7:33 PROSECTOR: NANCY DIEDRICH RECORDER: JACK SERF POST-FIX WEIGHER: NOT AVAILAoSl E PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME --------------------- (GRAMS) -------- TO BODY WEIGHT (XI -------- WEIGHT RATIO -------- s BRAIN (ON) 2.0041 SI44 % 1.0000 WE LIVER fLt) LUFNG (LU) 23.9691 1.3513 6.1522 % .3486 % 11.9000 WE .6778 WE L TESTIS TEO1 i.osol .4260 % .1212 WE NT: TESTIS TE 1.6212 .4161 % .8089 WE ACCESS/SEX ------- -01(-l--I-S-O-)-------------2-.-86-2-S-------------------.-7-34-7--%--------------1-.-42-8-3------------W-E ------------- ORGAN NAME 0 a a P A T H 0 L0 0 V 0 8 S E N V A T 1 0 N S 0 & h KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI( ------------------------------------------------------------------------------------------------------ KIDNEY(S) IKDI . . . . . . . . . I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GROSS: -LARGE PELVIS(ES) -LEFT; BOTH KIDNEYS COLLECTED -SEMIFLUID MATERIAL-PELVIS -LEFT: FILLED WITH CLEAR FLUID; BOTH KIDNEYS MICRO: -PRESENT -DILATATION.PELVIC LIVER (LI) . . . . . . . . . MICRO: -SLIGHT. iID6A@ . . . . . . . . -SLIGHT -NiCiOili.6OAG6LATiVi -HYPEIITNOPHY, HEPATOCELLULAR: CHARACTERIZED CENTT ILOSULATED PATTERN IN WHICH THE HEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BC ROUNDED GIVING THE CELLS A MORE "PLUMP' APPE THIS 'ENLARGED" APPEARANCE. THE CELLS ARE 07 THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ------ BRAIN (ON). - COAGULATING - OLD ICOI. LF. TESTIS (TEO). LUNG ILU). PROSTATE -- -- - -- - - IPRI, -SEMINAL-VESICLE-(SV).-URET INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA HAZLETON LABORATORIES AMERICA, MADISON, WISCONSIN U.S.A. INC. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?699 SEX: t4ALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED. SECOND INTER DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 TERMINAL BODY WEIGHT: DATE AND TIME OF NECROPSY: 02 14/91 1:03 PROSECTON: RUTH SCHMITZ RECORDER: JILL BEVA P-O-S-T--F-I-X---WE-I-G-H-ER-:---N-O-T--A-V-AI-L-A-O-(E-------------------PA-T-H-OL-O-G-IS-T-:----D-R.---T-OM---PA-L-M-ER------------------W-E-IG-1-4-E-R:---V-AL---QU-I-N-N-------- ORGAN NAME ABSOLUTE (GRORAGMASN) WEIGHT OR7G0ANBODWYEIGWHETIGHTRELATIVE ORWGEAINGHT TO RABTRIAOIN 0 ---------------------- -------- -------- -------- s ORAI LIVERN IBLRI LUNG LLUI 1.9812 23.SIS2 1.6440 .4384 % 5.2412 % .3638 % 1.0000 WE 11.9sso WE .8298 WE LF. TE TI OITEO11 1.7874 .30SS % .9022 WE AT. TESTIS ACCESS/SEX TE 0 (SO) 1,67so 2.5243 .3707 X ssse % .84s4 WE --------------------------------------------------------------------------------------1.-2-7-4-1-------------W-E------- I A a 0 P A TH 0 L 0 0 V 0 8 S E R V A T 1 0 N S A & A ORGAN NAME ---------------------- K-E-Y-W-OR-D-S-. ---S-E-VE-R-I-TY-.---D-I-S-TR-I-B-UT-I-O-N-----------GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI ------------------------------------------- GENERAL COMMENT LIVER ILI) GIGOCSI: -AL@ iliSOEt/bltdAiS NORMAL . . .. .. .. . .. . .. -THE- FO-LLOW-ING - MI@R6:'-iqlkTi4A@,'F6CAL' -SLIGHT -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C -INFILTRATES.MON.ON.UCL.EAR. . . .CELL -HYPE T ITOPHY HEPATOCELLULAR: CNARACTERIZED CENTR0ITOBULATED PATTERN IN WHICH THE HEPATO( A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 8 ROUNDED GIVING THE CELLS A MORE 'PLUMP" APP THIS_*ENLARGED" APPEARANCE. THE CELLS ARE 0 - --- --- -- --- - EX-AMIN-ATI-ON.-- BRAIN IBRI. COAGULATING GLD (CO), LF. TESTIS (TEO). LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE ISV). URE -------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7700 DATE OF DEATH: 03/2S/91 SEX: MALE DOSE GROUP: S STUDY DAY OF DEATH: 90 SACRIFICE STATUS: SCHEDULED TERMINAL SACR DATE AND TIME OF NECROPSY: 03 2S/91 7:21 STUDY WEEK OF DEATH: 14 PROSECTON: JACK SERFOITT TERMINAL 600Y WEIGHT: POST-FIX WEIGHER: NOT AVAILAO(E PAT14OLOCIST: DR. TOM PALMER RECORDER: JOE N. KIZ WEIGHER: JILL BEVAN ----------------------------------------------------------------------------------------------------------- ORGAN NAME ------------------- BRAIN LIVER ILBI IL LUNG ILU) LF- TESTIS TEO NY: TESTIS tEl ACCESS/SEX 010 Sol ABSOLUTE ORGAN WEIGHT (GRAHSI -------- 2.OS4S 34.0432 1.77S3 2.0474 l.os$4 4,1528 ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%I -------- .3071 % 6.0044 % .3178 % .3685 % .3SOO X 7434 % OROAN TO BRAIN WEIGHT RATIO -------- 1.0000 16.5701 .1641 .90as OS32 2.0213 0 ST --- WEI WEI WEI WEI WEI WEI ORGAN NAME PA TH0 L& 0 V KEYWORDS. SEVERITY, DISTRIBUTION --------------------- 0 6 S E ItV A T 1 0 N S * 0 a ONOSS FREE-TEXT COMENTS / HISTOPATHOLOGIC ----------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LI) (IA'OCSI-:ALE iliS6Ei/6NdAiS'N6RkAE . . . . .. . .. .. .. .. . ... .. .. . .. .. .. . MICRO: -SLIGHT -Hipinilt6phL@y hEiAiO@EiL6LAR: 'ChAiA6TiNiZiD' CENTRI OBU TEO PATTERN IliF@HICH THE NEPATOC A MORE HOMMENEOUS CYTOPLASM AND THE CELL 90 ROUNDED GIVIN43 THE CELLS A MORE 'PLUMP" APPE THIS 'ENLARGED* APPEARANCE. THE CELLS ARE OT THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - -- - - - - - BRAIN (ON). COAGULATING OLD (CO). LF. TESTIS ITEO). LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE (SVI. UNET -------------------------------------------------------------------------------------------------------------- to INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COI?01 SEX: MALE DOSE OROUP: S SACRIFICE STATUS: SCHEDULED FIRST POST-A DATE OF DEATH: OS/16/91 STUDY DAY OF DEATH: 148 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: OS 16/91 9:04 PROSECTON: JULIE A. LARSEN RECORDER: JOE M. KIZ POST-FIX WEIOHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PAL14ER WEIGHER: JACK SERFOR ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT oRaAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NA04E ---------------------- JORANS) -------- TO BODY WEIGHT (%I -------- WEIOHT RATIO -------- ST --- BRAIN 2.0221 .3733 % 1.0000 WEI LIVE 19.0210 3.SIJO % 9.4061 WEI LUNO (LU) 1.6734 .30 9 % .1273 WEI LF TESTIS TE 1.7353 .3203 X .8579 WEI ItT: TESTIS TE 1 1.7266 .3117 % IS36 WEI ACCESS/SEX 0 I0 olsol 4.9046 .0861 1 2.3752 WEI ---------------------------------------------------------------------------------------------------------------- 4 ob a P A T H 0 L 0 0 Y 0 0 S E R V A T 1 0 N S * 6 0 ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC -------------------------------------------------------------------------------------------------------- GENERAL COMMENT 0400CSI:-ALi iliS6Ei/61tdAiS'W6Ri4Ai . . . - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - UB,RTAEITNNoti"tlil, COAGULATING OLD (CO). LF. TESTIS (TEOI. LIVER (LI). LUNG (LU), PROSTATE IPR). SEMINAL VESICL uI -------------------------------------------------------------------------------------------------------------- an tn C30 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES . AMERICA. INC. MADISON. WISCONSIN U S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C@7702 SEX: NALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE OF DEATH: 02/13/91 STUDY DAY OF DEATH: SO STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02/13/91 1!43 PROSECTOlt: DENNIS HOFFMAN RECORDER: KEVIN OIL P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-O-T -A-V-A-IL-A-B-LE-----------------P-A-TH-O-L-OG-I-S-T:---O-N-.--T-OM--P-A-L-ME-R----------------W-E-I-GH-E-R-:--J-I-L-L--1-3E-V-A-N -------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 (GRAMSI TO BODY WEIGHT WEIGHT RATIO s ---------------------- -------- -------- -------- BRAIN 1811 1.9730 4263 % 1.0000 WE LIVER L LUNG 2S.0007 1.7193 S.'4021 % .3715 % 12.6714 WE .8714 WE LF. TE TI 1 1.5536 .33S7 % .7874 WE PT. TESTIS El I.SIS2 .3274 % .1010 WE -------AC-C-ES-S-/-SE-X---0-1-0---S-o-l--------------3-.-10-9-3-------------------.-6-71-8--%--------------I-.-SY-S-O-------------WE--------------- . a a a PA T H 0 L 0 0 Y 0 0 S E N V A T 1 0 N S A A A O-R-GA-N--N-A-M-E -------------K-E-Y-WO-R-D-S,---S-E-VE-R-I-TY-,---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-O-PA-T-"-OL-O-a-l------GENERAL COMMENT LIVER (LI) 04'03CSI-:AL@ iliS6Ei/61tdAiS'N6RkAi . .. . .. .. .. ... .. ... . .. .. . .. .. .. .. .. . .. .. . .. .. MICRO: -MINI14AL -"YPE TROPHY HEPAiO6ECL6LAR': 'ChAiA6TiltiZiD CENTRRILOBULATED PATTERN IN WHICH THE HEPATO A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 8 ROUNDED GIVING THE CELLS A MORE 'PLUMP* APP THIS 'ENLARGED" APPEARANCE, THE CELLS ARE 0 THE- FO-LLO-WING- -TISS-UES - WE-RE -UNR-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - BRAIN (13N).COAGULATING OLD (CO). LF. TESTIS ITEOI. LUNG (LU). PROSTATE (PR). SEMINAL VESICLE (SVI. UNE ----------------------------------------------------------------------------------------------------------- tn !n INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?703 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 TERMINAL 600Y WEIGHT: DATE AND TIME OF NECROPSY: 02 14/91 7:52 PROSECTON: RUTH SCHNITZ RECORDER: JILL BEVA -PO-S-T--F-I-X--W-E-I-GH-E-R-:--N-O-T--AV-A-I-L-AO-(-E----------------P-A-T-HO-L-O-GI-S-T:---D-R-.--T-O-M--PA-L-M-ER-----------------WE-1-4-2H-E-R-: --VA-L--Q-U-IN-N---------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 (GRAMS) TO BODY WEIGHT 4%1 WEIGHT RATIO s ---------------------- -------- -------- -------- BIAINR 1L9!l L VE 2.1139 27.2361 461 % S:47472 % 1.0000 WE 12.8143 WE LUNG ILU) 1.6909 .3568 % .7999 WE LF. TESTIS TE RT. TESTIS TEI0 1.7440 1.6816 .3680 % .3548 % .8250 WE .7955 WE ------A-C-CE-S-S-/S-E-X---0-1-0---I-SO-)-----------------2-.-1-2-59-------------------.-6-5-9-8-%---------------1.-4-7-8-7------------W-E ---------- a a * P A T H 0 LO 0 Y 0 8 S E R V A T 1 0 N S a a a oNaA-N --N-A-ME--------------K-E-Y-WO-R-D-S,---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-IC-------- GENERAL COMMENT c LIVER ILI) GI(olst:-ALi iliS6Ei/bRdAiS'W6Ri4AC . . .. .. .. .. . .. .. . .. .. .. . ... . . .. .. .. .. .. . .. .. .. .. . MICRO: -SLIGHT -HYPERTROPHY HEPATOCELLULAR: CHARACTERIZED CENTRILOBULATED PATTERN IN WHICH THE NEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 80 URETHRA JUR) --- -THE FOLLOWING ROUNDED OIVING THE CELLS A MORE "PLUMP" APPE THIS *ENLARGED' APPEARANCE. THE CELLS ARE Ot . .. .. ... .. .. . .; . .. .. .. . .. .. . .. .. . .. .. .. . .. .. .. . MICRO: -SECTION EXAMINED; TISSUE NOT PRESENT -- ---- --- -- -- ---- -- --- -- --- --- -TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN JOR). COAGULATING OLD (CO). LF. TESTIS (TEO). LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE (SVI --------------------------------------------------------------------------------------------------------------- 4,n Ul INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: CB?704 SEX: 14ALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST POST- DATE OF DEATH: 05/17/91 STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL 6ODY WEIO"T: DATE AND TIME OF NECROPSY: OS/17/91 1:17 PROSECTOR: OUT" SCHMITZ RECORDER: JOE N. KI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: DENNIS HOF --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OROAN WEIGHT RELATIVE OROAN TO BRAIN 0 ORGAN NAME ((IRAMSP TO BODY WEIGHT (%I WEIGHT RATIO s ---------------------- -------- -------- -------- BRAI 0 2.0053 33S? % 1.0000 WE LIVENR ILItlI 19.9150 3:34513 % 9.0661 WE LUNG (LU) l.s28s .2 9 % .7622 WE LF TESTIS TEO 1.6107 .2696 % .1032 WE ItT: TESTIS TEIISO) 1.6761 .2806 % .13se WE ACCESS/SEX @a 3.OOSI SI$2 % l.s438 WE --------------------------------------------------------------------------------------------------------------- A a a P A T " 0 L 0 0 Y 0 0 S E A V A T 1 0 N S ftA A ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION GROSS F#TEE-TEXT COMMENTS / HISTOPATHOLOGI ------------------------------------------------------------------------------------------------------- GENERAL COM14ENT GIGOCSI:-ALE iliS6Ei/6ft6AhS*N6NkAE - -THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNN-E14A-RKA-BLE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - BRAIN BA). COAGULATING GLD (COI, LF. TESTIS ITEO). LIVER (LI). LUNG (LU). PROSTATE (PRI. SEMINAL VESIC URETHRI JUR) INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. MADISON, WISCONSIN U.S.A. INC. --------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C67705 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE OF DEATH: 02/13/91 STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I DATE AND TIME OF NECROPSY: 02/13/91 9:14 PROSECTON: JULIE A. LARSEN TERMINAL 6ODY WEIGHT: RECORDER: KEVIN BIL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) 0110AN WEIGHT RELATIVE ORGAN TO BRAIN 0 ---------------------- -------- TO BODY WEIGHT 1%1 -------- WEIGHT RATIO -------- s GRAIN IBRI LIVER (LI) 2.0859 26.4825 .4285 % 1.0000 WE LUNG ILU) 1.5050 5.4401 % .3092 % 12.6960 WE .721S WE LF TESTIS OITEO IISOI RT. TESTIS TE l.esio 1.5958 .3404 % .3278 .7944 WE ACCE'SS/SEX 0 2.S026 % .5326 % .7650 WE 1.2429 --------------------------------------------------------------------------------------------------------WE------- L a a e P A T H 0 L,O 0 V 0 8 S E A V A T 1 0 N S 0 11* ORGAN NAME ---------------------- KEYWORDS. SEVERITY. DISTRIBUTION GROSS --------------------------------------- FREE-TEXT COMMENTS / HISTOPAT"OLOGI ------------------------------------------- GENERAL COMMENT LIVER (LI) GICOICSI: -ALE iliS6lEi/6R6AiS'N6lti4AE .. . ... .. . MICRO: :SLIGHT, iO@Ai MINIMAL -INFILTRATES.MOI#ONUCLEAR CELL -HYPE:TTOPHY HEPATOCELLULAR: CHARACTERIZED CENT I OBULATEI) PATTERN IN WHICH THE HEPATCN A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 6 ROUNDED GIVING THE CELLS A MORE *PLTIMP' APP THIS 'ENLAROED" APPEARANCE, THE CELLS ARE 0 - -THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATIO-N: - - - - - - - - - - - - - - BRAIN (IBRI. COAGULATING OLD (CO), LF. TESTIS ITEO). LUNG (LU), PROSTATE (PRI. SEMINAL VESICLE (SVI, UNE -------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANT14AL NUMBER: CG?706 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED FIRST POST-R DATE Of DEATH: OS/17/91 STUDY DAY OF DEATH: 140 STUDY WEEK OF DEATH: 22 TERMINAL 600Y WEIOHT: DATE AND TIME OF NECROPSY: 05 17/91 7:54 PROSECTOR: DEBBIE PIRKEL RECORDER: JOE M. KIZ POST-FIX WEIOHER: NOT AVAILAO(E PATHOLOGIST: ON. TOM PALMER WEIGHER: JACK SERFOR -------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE OROAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMS I -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- ST --- BRAIN (BRI 2.1826 .3810 % 1.0000 WE[ LIVER (LI) 21.S633 3.7039 % 9.8796 WEI LUNO (LU) 1.5680 .2737 % .7184 WEI LF TEST S ITTEEO11 1.7092 .2983 % .7131 WEI RT: TESTIS 1.6788 .2930 % .7692 WEI ACCESS/SEX 0 0 Sol 3.429S s986 % l.s7l3 WEI ---------------------------------------------------------------------------------------------------------------- aa& PA T"0 L0 G Y 08 S E4 VA T 10 NS *6 4 ORGAN---N-AM-E--------------K-E-Y-W-OR-D-S-, --S-E-V-ER-I-T-Y,---D-IS-T-R-IB-U-T-IO-N----------O-R-O-SS---FR-E-E--T-E-X-T--C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-H-OL--O--G-I-C---- GENERAL COMMENT : GIGO(S;I-:ALi iliS6Ei/61tdAiS'N6Ri4Ai . . . . - -THE- FO-LLO-WINO- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - AIN IBR). COAGULATING OLD (CO). LF. TESTIS (TEO). LIVER (LIJ. LUNG (LU). PROSTATE (PRI. SEMINAL VESICL UB:ETHR (UR) --------- ---------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CIIYO? SEX: MALE DOSE GROUP- 5 SACRIFICE STATUS: SCHEDULED SECOND INTERI DATE OF DEATH: OF02412491 STUDY DAY OF DEATH: SS DATE AND TIME EC OPSY: 02/12/91 7:34 ST6DY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: POST-FIX WEIOHER: NOT AVAILABLE PROSECTON: NA CY DIEDRICH PATHOLOGIST: "DR. TOM PALMER RECORDER: DENNIS HOF --------------------------------------------------------------------------------WE-I-G-HE-R-:---PA-T-H-OL-O-GY---D--------------- ORGAN NAME ---------------------- BRAIN 6 LIVER ILtl LUNG ILU) LF TESTIS ITE RT: TESTIS TEIOI ACCESS/SEX 0 0 Sol ABSOLUTE ORGAN WEIGHT (GRAMS) -------- 2.1931 30.2278 l.6s64 1.7721 1.7930 3.1442 ORGAN WEIOHT RELATIVE TO BODY WEIGHT 1%1 -------- .4410 % s.o7s9 % .3329 % .3562 z .3604 % .6320 % ORGAN TO BRAIN WEIOHT RATIO -------- 1.0000 13.7787 -ISSO .1071 .8173 1.4332 0 ST ... WEI( WEI( WEI( WEIG WEIG WEIG ORGAN NAME ------- ------GENERAL COMMENT P A T " 0 LISKO Y KEYWORDS. SEVERITY. DISTRI BUT 08 S EA VA T10 NS a aa GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGIC ----------------------------------7 ------- LIVER ILI) 01'03CSI-:ALi iliS6Ei/61tdAiS'N61tkA@ .. .. .. ... . .. .. .. . . MICRO: -SLIGHT .. .. . .. . .... .. .. . .. .. . .. .. .. . .. .. .. . ... .. -HYPENTROPHY HEPATOCILLULAR: CHARACTERIZED 8 CENTAILOBULATED PATTERN IN WHICH THE NEPATOCY A "ONE HOMOGENEOUS CYTOPLASM AND THE CELL BON ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPEA - - - - - - - - - - - - - - - --- - - - - - - TH-IS -"EN-LAR-GED'- A-PPE-ARAN-CE.- T-HE -CEL-LS -ARE- OT-H THE FOLLOWINO TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (84). COAGULATING OLD ICO). LF. TESTIS ITIO), LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE (SVI. UNETH ---------------------------------------------------------------------------------------------------------------- in gn a% APPENDIX D INDIVIDUAL AN114ALPATHOLOGY DATA HAZLETON LABORATORIES .AMERICA. INC. MADISON. WISCONSIN U S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER:C67701 SEX: 14ALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE OF DEATH: 02/13/91 STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I TERMINAL AODY WEIGHT: DATE AND TIME OF NECROPSY: 02/13/91 7:42 PROSECTON: RUTH SCHNITZ RECORDER: KEVIN OIL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. T014 PALMER WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT OIIGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 - -------------- (GRAMS) -------- TO BODY WEIGHT 1%) -------- WEI-GH-T---R-AT-I-O s BRAIN (OR) 2.2070 .43832 1.0000 WEI LIVER (LI) LUNG ILU) 24.6949 l.s957 4.8823 % .31SS % 11.1894 WEI .7230 WEI LF TESTIS TEO 1.6074 .3178 % .1213 WEI NT: TESTIS T El l.s9l7 .3147 % .1212 WEI ACCESS/SEX 010 ISO) 2.SIS3 --------------- ------------------.-5-171-% --------------I.-II-S-O------------W-E-I--- ORGAN NAME a8 a PA TH0 L00 Y KEYWORDS. SEVERITY. DISTRIBUTION 0 8 S E N V A T 10 N S A 6 6 GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC -------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER ILI) OI(OICSI:-AL@ fliS6Ei/6NdAiS'N6Wi4AE ... . .. . .. ... .. .. .. .. .. . .. .. . .. . .. .. . ... . .. .. .. . .. .. .. .. MICRO: -MINIMAL -HYPER TROPHY EPATOCELLULAR: CHARACTERIZED CENTNILOBULATE"D PATTERN IN WHICH THE HEPATDC A MORE NOMOOENEOUS CYTOPLASM AND THE CELL BO NOI)NDED GIVING THE CELLS A MORE *PLTMP" APPE THIS "ENLARGED' APPEARANCE. THE CELLS ARE OT THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN IBR). COAGULATING GLO ICO). LF. TESTIS (TEO). LUNG ILU). PROSTATE (PA). SEMINAL VESICLE (SV). UNET --------------------------------------------------------------------------------------------------------------- APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?709 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE or DEATH: 02/12/91 STUDY DAY Or DEATH: 55 STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02/12/91 9:15 PROSECTOlt: NANCY DIEDRICH RECORDER: DENNIS NO P-O-S-T--F-I-X --W-EI-G-H-ER-:---N-OT--A-V-A-IL-A-1-3L-E----------------P-A-TH-O-L-OG-I-S-T:---D-R-.--T-OM--P-A-L-ME-R----------------W-E-I-GH-E-R:---P-A-T-HO-L-OG-Y----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 (GRAMSI TO BODY WEIGHT 1%1 WEIGHT RATIO s ---------------------- -------- -------- -------- BRIAINR 1L6Rli 2.1310 5843 % 1.0000 WE L VE 23.2540 6 37:2 % 10-9122 WE LUNG ILU) 1.314S :31 6 % .6497 WE LF TESTIS TEO1 RT: TESTIS TE 1.6261 1.5571 .4459 % .4271 % .7631 WEI .7310 WEI ------A-C-C-ES-S-/S-E-X---0-1-0---IS-O-)-----------------2-.-3-4-07-------------------.-0-4-1-8 -%--------------1-.-0-9-14-------------W-EI----------- a 4a PA T" 0 L00 Y 0 8 Sf N VA I10N S a * & O-R-GA-N---MA-H-E--------------KE-Y-W-OR-D-S-. --S-E-V-ER-I-T-Y.---D-IS-T-R-IB-U-T-IO-N----------G-R-O-SS---FR-E-E--T-E-X-T--C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-H-OL-O-G-IC--- LIVER (LI) . . . . . . . . . . . . . . . . . . . . . . . . . GROSS: -DIFFUSELY DARK -AiL*L6BiS: iltbwi MICRO: -SLIGHT -HYPENTROP"Y "EPATOCELLULAR: CHARACTERIZED CENTRILOBULATED PATTERN IN WHICH THE NEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BO ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPE THIS "ENLARGED" APPEARANCE. THE CELLS ARE OT THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (OR). COAGULATING OLD (CO), LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SV). URET -------------------------------------------------------------------------------------------------------------- c4nLn co APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER:CS?710 SIX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED SECOND INTEO DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 TERMINAL IODY WEIGHT: DATE AND TIME OF NIECKOPSY: 02(14/91 7:24 PROSECTOlt: KEVIN BILLINGS RECORDER: JILL BEV POST-FIX WEIG14EN: NOT AVAILAB E PAT14OLOGIST: DR. TOM PALMER WEIOHER: VAL QUINN --------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIOHT ORGAN WEIGHT RELATIVE OROAN TO BRAIN ORGAN NAME IOWANS) TO BODY WEIGHT (%I WEIGHT RATIO s ----------------- -------- -------- -------- BRAIN (BPI 2.0112 .4S$3% 1.0000 WE LIVER (LI) 31.2847 6.9048 % IS.0320 WE LUNG (LU) 1.4613 .3241 % .7oss WE LF. TESTIS (TEO) 1.8449 .4072 % .816$ WE PT. TESTIS ITEIISO) 1.6789 .3?OS % .8067 WE ACCESS/SEX 0 0 3.0033 .9628 % 1.4431 WE --------------------------------------------------------------------------------------------------------------- a ita P A T H 0 L-0 0 V 0 8 S E N V A T 1 0 N S & 0 A ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOCII ----------------------------------------------------------------------------------------------- GENERAL LIVER COMMENT aleoCSI: -ALE iliS6Ei/6R6AiS'f#6Ri4AC (LI) . . . . . . . . . . . . . . . . . . . . . . . . . MICRO: -SLIGHT :HiPiltiPt6P*"V''HE'PAT'OC'E'LL6LAR':ChAiAdTiNiZiD CENTRI LOBULATED PATTERN IN WHICH THE 14EPAT A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 0 ROUNDED GIVING THE CELLS A HOPE *PLtlt4P" APP THIS 'ENLARGED' APPEARANCE. THE CELLS ARE THE- FO-LLO-WINO- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - 7 BRAIN (OR). COAGULATING OLD (CO). Lf. TESTIS ITEO). LUNO (LU). PROSTATE IFRI, SEMINAL VESICLE (SVI. UNE ------------------------------------------------------------------------------------------------------------- tn 4" 40 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMALNUMBER-C.o??Ii DATE OF DEATH: 02/14191 SEX: 04ALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED SECOND INTERI STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 TERMINAL 600Y WEIGHT: DATE AND TIME OF RECROPSY: 02/14/91 8:25 PROSECTOR: KEVIN BILLIMOS RECORDER: JILL BEVAI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: VAL QUINN ----------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT OROAN TO BRAIN WEIGHT RATIO 0 --------------------- -------- -------- -------- ST --- BRAIN LIVER LUNG LIR J ILU) 1.0929 23.6896 .9224 4443 % !5:2820 % .20S? % 1.0000 WEI 11.8070 WEI .4628 WEI LF. TESTIS AT. TESTIS TEO TEIISO, 1.8353 1.1301 .4092 S .4082 % .9209 WEI .9187 ACCESS/SEX 010 ------- 2.4993 SS?3 % WEI 1.2S41 WEI ------------------------------------------------------------------------------------------------------- ORGAN NAME a a e PA T H 0 L 0 0 Y 0 8 S E R V A T 1 0 N S a a a KEYWORDS. SEVERTTY, DISTRIBUTION -------------------------------------------------------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-H-OL-I-O--G-I-C----- GENERAL COMMENT LIVER (LI) . G.AG.OCS.I:.-A.L@. i.li.S6.Ei/.6R.6A.hS*.N6.lt.kA@. . .. . .. . . . . . MICRO: -MINIMAL -HiPiR'TR*OP'HY' PROSTATE (PRI CENTRILOSULATED PATTERN IN WHICH THE NEPAIOC A NNE HOMOGENEOUS CYTOPLASM AND THE CELL 80 ROUNDED GIVING THE CELLS A MORE 'PLUMP' APPE . . . . . . . . . . . . . . . . . . . . . . . . .TH.IS. "ENLARGED' .. .. . APPEARANCE. .. .. .. THE .. CELLS . .. ARE .. OT .. MICRO: -MODERATE. MULTT-FOCAL -INFLAMMATION. CHRONIC ACTIVE THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON-- - - - - -------BRAIN -JO-R)-.--C-OA-G-U-L-AT-I-N-G--O-L-D--(-C-O)-.--L-F-.-T-E-S-T-IS---(T-E-O-)-.--LU-N-G--(-L-U)-.--S-E-MI-N-A-L--V-ES-I-C-L-E--(-S-V-I.--U-R-ET-H-R-A--I-U-R-)------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7712 SEX: MALE DOSE GROUP; S SACRIFICE STATUS: SCHEDULED TERMINAL SAC DATE OF DEATH: 03/26/91 STUDY DAY OF DEATH: 91 STUDY WEEK OF DEATH: 14 TER14114AL 6ODY WEIGHT: DATE AND 71ME OF NECIOPSY (26/91 7:37 PROSECTON: JULIE A. LARSEN RECORDER: JILL BEVA P-O-S-T--F-I-X--W-E-IO-H-E-R:---N-O --A-V-A-I:L-A-0S-3 -E----------------P-AT-H-O-LO-G-I-ST-:---D-R-. --T-OM--P-A-LM-E-R----------------W-E-I-GH-E-R-: --J-O-E--M.--K-I-Z--------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE OftOAN TO BRAIN 0 OROAN NAME ---------------------- JORANS) -------- TO BODY WEIGHT 1%) -------- WEI-O-HT---R-AT-I-O s BRAIN (Bit) 2.2038 .37?S % 1.0000 WE LIVER ILI) LUNG ILU) 33.3$OS 1.6828 5.7178 % .28'82 % IS.1468 WE .7636 WE LF TEST S TEO) 1.8700 .3217 % .8522 WE RT: TESTis TEI 1.0821 .3191 x .1453 WE ------A-C-C-ES-S-/S-E-X---0-I-0---IS-O-)-----------------4-.-1-2-SS-------------------.-7-0-6-7 -%---------------1.-8-7-2-0------------W-E ---------- * a a P A T N 0 L.0 G Y 0 8 S E P V A T 1 0 N S A * & O-R-G-AN---NA-M-E-------------K--E-Y-W-OR-D-S-, --S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T --C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-HO-L-O-OT-------GENERAL COMMENT LIVER (LI) GAOGCSI:-ALi iliS6Ei/6RdAiS*N6RAAi -SLIO"T PROSTATE (PRI . . . . . . . . MICRO: -MODERATE. iO@Ai LliFiLTRATES,MONONUCLEAR'CILL -HYPE:TROPUHY "EPATOCELLULAR: CHARACTERIZED CENT ILOS LATED PATTERN IN WHICH THE HEPAiroo A "ORE HOMOGENEOUS CYTOPLASM AND THE CELL 6 MOUNDED GIVING THE CELLS A MORE "PLLlt4P'APP THIS 'ENLARGED' APPEARANCE. THE CELLS ARE 0 -INFLAMMATION.'CHRONIC'ACTIVE ... THE- FO-LLO-WING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - -------BR-A-I-N--(-84-)-,--C-OA-G-U-L-A-TI-N-G--O-L-D--(-CO-)-.--L-F-. -T-E-S-T-I-S --(-TE-O-)-.--LU-N-G--(-L-U-)-. -S-E-M-I-NA-L--V-E-S-I-CL-E---(S-V-I-,--UR-E-T-H-RA---(-U-RI-------------------- in INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67713 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED FIRST INTERIM DATE OF DEATH: 01/25/91 STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 0 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 01(2S/91 8:00 PROSECTON: JULIE A. LARSEN RECORDER: NANCY DIED POST-FIX WEIGHER: NOT AVAILAS E PATHOLOGIST: DO. TOM PALMER WEIGHER: PATHOLOGY D ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OftOAN NAME ---------------------- (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0 ST --- 13PAIN (891 2.2337 SO$4 % 1.0000 WEI LIVER (LIJ 2S.8610 6.1217 % II.Si?7 WEI LUNG (LU) 1.462S .9 8 % OS41 WEI LF TESTIS ITEO i.esel .4443 % .7420 WEI IT.' TESTIS (TEI 1.6381 .4381 % .7334 WEI ACCESS/SEX ONG SO) 2.SISS .0739 % 1.1262 WEI ---------------------------------------------------------------------------------------------------------------- * & a P A T H 0 L 0 0 Y 0 8 S E A V A T 1 0 N S A 0 ft ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION ONOSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC -------------------------------------------------------------------------------------------------------- KIDNEVIS) IKDI . . . . . . . . . . . . . . . . . . . . . . . GROSS: -LARGE PELVISIES) -* ftiOiT:iOiH'C6LiE6Tit)* MICRO: -PRESENT -DILATATION,PELVIC LIVER (LI) :IiFiLtitATiS:m6N6N6ciEiR*CiLE -SLIGHT -HYPEITROPHY HEPATOCELLULAR: CHARACTERIZED CENT ILOBULATED PATTERN IN WHICH THE NEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 80 ROUNDED GIVING THE CELLS A MORE "PLUMP' APPE THIS "ENLARGED' APPEARANCE. THE CELLS ARE OT THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN 18N). COAGULATING OLD ICO). LF. TESTIS (TEO). LUNG (LU), PROSTATE (PRI, SEMINAL VESICLE ISVI. UPET - ---------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. MADISON, WISCONSIN U.S.A. INC. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?714 DATE OF DEATH: 03/25/91 SEX: MALE STUDY DAY OF DEATH: DOSE GROUP: 5 SACRIFICE STATUS: Of STUDY WEEK OF DEATH: 14 SCHEDULED TERMINAL SACR TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 03 2S/91 9:58 PROSECTOR: JACK SERFORT RECORDER: JOE N. KI@ POST-FIX WEIGHER: NOT AVAILAOCE PATHOLOGIST: DR. TOM PALMER WEIGHER: STEVE VAN i ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- s --- BRIAIEN 18LRIJ 2.1574 5192 % 1.0000 WEI LVR 28.6577 6:8972 % 13.2834 WEI LUNG ILU) 1.4941 .3506 % .6925 WEI LF TESTIS ITE 1.6633 .4003 % .7710 WEI AT. TESTIS ITEI0 I l.?S22 .4217 % .8122 WEI -------A-CC-E-S-S/-S-E-X ---O-R-O ----S-o-l---------------3-.-2-74-1---------------------.-7-8-80--%----------------i.-s-il-e --------------W-E-I -------- a a * P A I H 0 L0 0 Y 0 9 S E P V A T 1 0 H S a & * ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC ---------------------- ----------------------------------------------------------------------------------- GENERAL COMMENT c GA'ost: -A.Li iliS6E.i/6lt6AAS'N6Ri4Ai LIVER ILI) MICRO: -041MINAL. F6CAL* -liFiLiftATiS*.H6N6"6C@EAN'CiL@ -SLIOHT -HYPEOITROPHY HEPATOCELLULAR: CHARACTERIZED CENTIT ILOSULATED PATTERN IN WHICH THE HEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BO ROUNDED GIVING THE CELLS A MORE *PLUMP" APPE THIS *ENLARGED' APPEARANCE, THE CELLS ARE OT THE- FO-LLOW-INO- T-ISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - BRAIN IHN). COAGULATING OLD ICOI. LF. TESTIS ITEO), LUNG (LU). PROSTATE IPRI, SEMINAL VESICLE ISVI. URET ---------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES .AMERICA, MADISON. WISCONSIN U S.A. INC. -------------------------------------------------------------------------------------------------------------- ANT14AL NUMBER: C$7715 DATE OF DEAT14: 01/24/91 SEX: MALE DOSE GROUP: 5 STUDY DAY OF DEATH: 36 SACRIFICE STATUS: SCHEDULED FIRST INTER DATE AND TIME Of NECROPSY: 01/24/91 8:14 STUDY WEEK OF DEATH: 6 PROSECTON: DENNIS HOFFMAN TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PAT14OLOGIST: ON. TOM PALMER RECORDER: VAL QUIN ---------------------------------------------------------------------------------------W-E-IG-H-E-R:----DE-B-B-IE---P-I------- ORGAN NAME A13SOLUTE ORGAN WEIGHT (GRAMSI OICAN WEIGHT RELATIVE ORGAN TO BRAIN ---------------------- -------- 0 BODY WEIO"T 1%) -------- WEIOHT RATIO -------- s BRAIN LIVER 1.9928 .5139 % 1.0000 WE LUNG fLU) LF. TESTIS (TEO) 26.04S? 1.4185 1.7886 0.7183 % .36se % .4612 % 13.0699 WE .7118 WE .897S NT. TESTIS TEIISO, ACCESS/SEX Oia 1.8534 .4779 % WE .9300 WE ----------------------------------------2-.-77-8-5---------------------.-7-t-es--%----------------1.-3-9-4-3-------------W-E------- aba PA I OROAN NAME KEYWORDS. SEVERITY, 10 LIO 0 Y DISTRH BUT 6M 0 8 S E R V A T 10 N S 6* A ---------------------- ---------------------------------------G-R-O-SS---F-R-E-E--T-E-XT----CO-M-M-EN-T-S----/---H-I-ST-O-P-A-TH-O-L-OG-I----- GENERAL COMMENT LIVER (LI) GAOGCSI: -ALE iliS6Ei/6ftdAiS'N6RkAi . .. .. .. .. .. . .. .. .. .. .. . .. .. MICRO: -SLIGHT -"YpiRilt6PAY' AIEOAiO@EiL6LAR: CHAK'AC*TE*R*IZED CE"IritILOSULATED PATTERN IN WHICH THE HEPATO A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 9 ROUNDED GIVING THIS "ENLARGED* THE CELLS A MORE "PLUMP" APP APPEARANCE. THE CELLS ARE 0 THE FOLLOWIN2 TISSUES WERE UNREMARKABLE AT MICROSCOPIC EFAMINATION: BRAIN (B ). COAGULATING GLO (CGI. LF. TESTIS (TEO). UNG (LU). PROSTATE (Pt). SEMINAL VESICLE (SVI. URE --------------------------------------------------------------------------------------------------------------- Ln INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES A"ERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANit4AL NUMBER: COI?16 DATE OF DEATH: 03/22/91 SEX: 14ALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED TERMINAL SAC STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 DATE AND TIME OF NECROPSY: 03/22/91 9:05 PROSECTOR: KEVIN BILLINGS TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER RECORDER: JOE M. KI WEIGHER: JILL BEVAN ------------------------------------------------------------------------------------------------------------ ORGAN NAME ABSOLUTE JOROARMGSA)N WEIGHT ORTGOANBODWYEIGWHETIGHRTELAITIIIVE ORWGEAINGHTTO RABTRIAOIN 0 ---------------------- -------- -------- -------- s BRAIN LIVER 2.2060 21.3482 .4747 % 1.0000 WE LUNG ILU) LF TESTIS TEO1 1.5976 1.6894 4.5935 % .3438 % .3635 % 9.0764 WE .7242 WE .7658 WE RT: TESTIS TE ACCESS/SEX 010 ISO) 1.6342 2.8111 .3SI7 % .6049 % .7401 WEI 1.2743 WEI ---------------------------------------------------------------------------------------------------------------- ORGAN NAME PA TH 0 L00 Y 0 8 SE RV A T 10N S *4 6 KEYWORDS. S6EV*ER*ITY. DISTRIBUTION G-E-NE-R-AL---C-O-M-ME-N-T ---------------------------------------------G--R--O-S-S---F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---"-IS-T-O-PA-T-H-OL--O--G--IC---- LIVER ILI) GAGOCSI:-ALE itiS6Ei/6RdAiS*N6RAAE . .. ... .. .. . .. . .. .. .. .. . .. . .. .. . .. .. .. .. . .. .. . ... MICRO: -MINIMAL -HYPERTROPHY NEPATOCELLULAR: CHARACTERIZED CENTR ILOBULATED PATTERN IN WHICH THE NEPATOC A MORE NOMOOENEOUS CYTOPLASM AND THE CELL 80 ROUNDED OIVING THE CELLS A MORE *PLTOMP' APPE THIS "ENLARGED" APPEARANCE. THE CELLS ARE OT THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN (OR). COAGULATING OLD (CO). LF. TESTIS ITEO). LUNG ILU). PROSTATE (PR), SEMINAL VESICLE (SVI. URET ---------------------------------------------------------------------------------------------------------------- gn INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES .AMERICA, INC. MADISON. WISCONSIN U S.A. -------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C$7717 DATE OF DEATH: 01/23 91 SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED. FIRST INTER STUDY DAY OF DEATH: 35 STUDY WEEK OF DEATH: 5 TERMINAL BODY WEIGHT: DATE AND TIME OF NEC40PSY: 01/23/91 7:59 POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: JULIE A.*LARSEN RECORDER: JILL BEV -------------------------------------------PA-T-14-O-L-OG-I-S-T:---DR-.--T-O-M--P-AL-M-ER-----------------W-EI-G-H-ER-:---G-AI-L--M-.--N-------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ---------------------- -------- TO BODY WEIGHT IZI -------- WEIGHT RATIO -------- s BRAIN (BRI LIVER ILI) 2.0368 2S.ISOI .5642 % 1.0000 w LUNG ILUI LF. T T 1.3688 $.!$is % .7 2 % 12.3S26 w .6720 WE I (TEO) RT. TESTIS TEIISOI 1.0172 I.OS43 .4480 % .7940 WE -------AC-C-E-SS-/-S-EX---O-I-G-------------------2-.-2-71-7-------------------..-46-s3-$132--%%--------------1-..-18-118-282-------------WWEE--------------- KEYWORDS. ; O-R-GA-N---NA-M-E------------------------[-VE-R-I-T-Y,---DI-S-T-R-IB-U-T-IO-N----------G-R-OS-S FREE-TEXT COMP4ENT; /* HISTOPATHOLOGI GENERAL COM14ENT c ------------------------------------------- LIVER ILI) GIGOSI: -ALC iliS6Ei/6R6AiS*N6ltilA@ IFYPE T ROPHY HEPATOCELLULAR: CHARACTERIZED CENTRRILOBULATED PATTERN IN WHICH THE HEPAT A 14ORE HOMDOENEOUS CYTOPLASM AND THE CELL 8 ROUNDED OIVING THE CELLS A MORE *PLUMP' APP THIS 'ENLARGED' APPEARANCE. THE CELLS ARE 0 THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNN-EMAR-KA1-3LE- AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - -------B-R-A-I-N--J-O-R)-.--C-O-AG-U-L-A-T-IN-G--G-L-D--I-C-O-).--L-F-.--T-ES-T-I-S--I-T-EO-)-,--L-U-N-G--IL-U-I-,--P-RO-S-T-A-T-E --(F-R-I-.--S-EM-I-N-A-L--V-ES-I-C-L-E--(-S-VI-.--U-R-E-------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES . AMERICA. INC. MADISON. WISCONSIN U S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COIIII SEX: t4ALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED SECOND INT[RT DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02 14/91 1:40 PROSECTOlt: DENNIS HOFFMAN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER: VAL QUINN ---------------------------------------------------------------------------------------------------------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT 1%) ORGAN TO BRAIN WEIGHT RATIO 0 ------------------- -------- -------- -------- ST --- BRAI 2.1511 .4414 % 1.0000 WEI LIVERN 34.OGS3 7.1014 % IS.8362 WEI LUNG ILU) l.s7l7 .3276 % .7306 WEI LF TESTIS 1,TE11O JIT: TESTIS E 1.4320 1.5203 .2915 % .3189 % .6657 WEI .7041 WEI -------AC-C-ES-S-/-SE-X---0--0---S-o-l----------------2-.-9-6-80-------------------.-6-1-8-4-1--------------1-.-3-7-91-------------W-EI----------- ORGAN NAME * 0A PA TH 0 L00 Y KEYWORDS. SEVERITY. DISTRIBUTION -------------------- 0 6 S I R V A T 10 N S A 0 0 GROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGIC ---------------------------------------------------------------------------------- OENERAL COMMENT LIVER ILI) GI(OICSI:-AL@ iliS6Ei/61tdAiS*?16fti4AC . .. ... .. . MICRO: -SLIGHT. iO@Ai -SLIGHT -liFiLiltiliS:Pl6N6lf6CCEAlt'CiL.i. . . . . -"YPERT ITOPHY NEPATOCELLULAR: CHARACTERIZED CENTRILOBULATEO PATTERN IN WHICH THE HEPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 80 RTOHUINSDED'ENLGAIRVGIENDG' TAHPEPECAERLALNSCE.A MOTRHEE C"EPLLLUSMP*AREAPPOET THE FOLLOWING TISSUES WERE UNNEMARKA13LE AT MICROSCOPIC EXAMINATION: -------B-R-A-I-N--I-BR-I-.--C-OA-G-U-L-A-TI-N-G--G-L-O--(-C-O-),--L-F-.--T-ES-T-I-S--I-T-EO-)-.--L-U-NG---(L-U-)-,--PR-O-S-T-A-TE--(-P-R-)-.--SE-M-I-N-A-L --VE-S-I-C-LE---I-SV-I-.--U-R-ET--------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES .AMERICA. INC. MADISON. WISCONSIN U S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CO?Ilg DATE OF DEATH: SEX: MALE DOSE GROUP: S SACRIFICE STATUS: SCHEDULED SECOND INTERI STUDY DAY OF DEATH: 55 DATE AND TIME OF024EIC2RI09PIS0Y2:/12/91 7:20 PROSECTOR STUDY WEEK OF DEATH: I : RUTH SCHMITZ TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER RECORDER: DENNIS HOF ---------------------------------------------------------------------------------WE-I-GH-E-R-: ---PA-T-HO-L-O-GY---D--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT _!GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ---------------------- ------ TO BODY WEIGHT -------- WEIGHT RATIO ---- --- ST BRAIN LIIVER I OL1t1 j 2.1708 30.9516 .4S70 % 1.0000 WEI LUNG ILU) LF TESTIS TEO1 l@5670 1.6091 0,4998 % .3290 % .3378 1 14.2221 WEI .7199 WEI .7392 WEI ItT: TESTIS TE ACCESS/SEX 010 ISO) 1.6220 2.0267 .3405 % .74SI WEI ------------------------------------------------------------.6-1-4-S--% --------------1-.3-4-4-5------------W-E-I --------------- ORGAN NAME - ------------ MEYWORDS, GENERAL COMMENT P A I H 0 L 0-0 Y 0 8 S E A V A T 1 0 N S a a 0 ;EV*ER*ITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC ------- ------------------------------ LIVER ILI) GIOSSCI: -iLi iliS6Ei/61tdAhS'N6RkAE ' . .. .. . . M.ICR.O:. ... . . -MINIMAL. . .. FOCAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -SLIGHT -INFILTRATES,MONOMUCLEAR CELL -HYPEITTROPHY HEPATOCILLULAR: CHARACTERIZED CENTRILOBULATED PATTERN IN WHICH THE HIPATOC A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 00 WOUNDED GIVING THE CELLS A MORE 'PLUMP* APPE THIS "ENLARGED" APPEARANCE. THE CELLS ARE OT THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - --------BR-AIN ION), COAGULATING GLD (CGI. LF. TESTIS ITEO). LUNG (LU). PROSTATE IPRI, SEMINAL VESICLE (SVI, URET ------------------------------------------------------------------------------------------------- Ln Ch CD INDIVIDUAL APPENDIX D AN114AL PATHOLOGY DATA HAZLETON LABORATORIES . AMERICA. INC. MADISON. WISCONSIN U S.A. -------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C$7720 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED TERMINAL SA DATE OF DEATH: 03/22/91 STUDY DAY OF DEATH: 93 DATE AND TIME OF NECROPSY: 03/22/91 7:12 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: RUTH SCH04ITZ PATHOLOGIST: DR. TOM PALMER RECORDER: JOE M. K --------------------------------------------------------------------------W-E-I-GH-E-R-:--J-I-L-L--B-EV-A-------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN NAME --------------------- (GRAMSP -------- TO BODY WEIGHT (Z) -------- WEIGHT RATIO -------- s BRAIN ILBIRJ LIVER 1.0403 36.3471 2948 % 5*5230 % 1.0000 WE LUNG (LU LF TESTII ITEOII I.SO44 .2286 % 11.7327 WE .77s3 WE RT ' TESTIS 1.7913 1.1786 .2722 % .2703 % .0232 WE .9167 WE -------AC6ESS/-SE-X---0-0----S-O)---------------4.-4-0-s-o-------------------.6-6-9-S-I-x--------------2.-2-7-0-7------------W-E ------------- ORGAN NAME 04ANDIO/CERV a a * P A T N 0 L.0 0 V 0 8 S E R V A T 1 0 N S It* KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / A HISTOPATHOLOOI - ------------------- ---------------------------------------- - - - - - - MICRO: -SLIGHT -HiPiRRiR6Lphy, *"E*PAT,OC*E*LL,ULA**C:*HAR,AC,TE*RZlE,D C ENT I OBULATED PATTERN IN WHICH THE "EPAT A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 0 ROUNDED GIVING THE CELLS A MORE 'PLUMP' APP LN THIS 'ENLARGED" APPEARANCE. THE CELLS ARE 0 MICRO: -MODERATE, . . . . . . . . . . . . -.R.10."T':IS'X'1'0 'MM-(F-ORCOMPARISON. MULTI-FOCAL (CAP 61 HYPERPLASIA. LYMPHORETICULAR CELL LEFT'IS THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: - -------B-R-A-I-N--(O-R-)-.--CO-A-G-U-LA-T-I-N-G--O-LD---IC-O-)-.--L-F.--T-E-S-TI-S--(-T-E-O-I.--L-U-N-G--(-LU-)-.--P-R-OS-T-A-T-E--(-P-RI-,--S-EM-I-N-A-L--V-ES-I-C-L-E--I-S-VI-,--U-RE---------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?721 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED. SECOND INTER DATE OF DEATH: 02/13 91 STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I TERMINAL BODY WEIGHT: DATE AND TIME OF NEC40PSY: 02/13/91 7:17 POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: DENNIS HOFFMAN RECORDER: KEVIN BIL ------------------------------------P-AT-H-O-L-OG-I-S-T:---D-R-.--?-(*-4-P-A-L-ME-R----------------W-E-I-GH-E-R-;--J-I-L-L--B-EV-A-N-------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN --------------------- -------- -------- WEI-GH-T---R-AT-I-O s BRAINR 1L0!, LIVE 2.0155 .4615 % 1.0000 WE LUNG (LU) 28.5484 1.41SO 6.3174 % .3133 % 13.6190 WE .0711 WE LF. TESTIS (TEO) NT. TESTIS TEIISOI 1.8756 1.4333 .4150 % .8994 WE ACCESS/SEX o@G 2.3522 .4057 % .6312 % .8791 WE 1.3676 WE -------------------------------------------------------------------------------------------------------------- ORGAN NAME GENERAL COMMENT KEYWORDS, SaEVkER0ITY. P DAISTTRHIBU0TILO.N0 0 Y 0 0 S E R V A T 10 N S * a GROSS FREE-TEXT COMMENTS /4 HISTOPAT"OLOOI ------------------------------------- . 1.34.0'.Cs.l-:i.Li. i.li.S6.lE.i/.i).lt6.Ai.S'.N6.Ri.4A.k . . . . . . . . . .. .. . . . .. . .. . . . . . . . . . . .. LIVER ILI) MICRO: -SLIGHT -HYPE;T!OOPgYA HEPATOCELLULAR: CHARACTERIZED CENT 1 0 L TED PATTERN IN WHICH THE NEPATO A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 8 ROUNDED GIVING THE CELLS A MORE 'PLUMP- APP THIS 'ENLARGED' APPEARANCE. THE CELLS ARE 0 THE- FO-LLOW-ING- -TISS-UES- WE-RE-UNRE-MARK-ABL-E - AT- P4-1CRO-SCO-PIC - EX-AMIN-ATI-ON -- - - - - - - - - - - - - - BRAIN -------------- IOR). COAGULATING GLD ICO). LF. TESTIS ------ -------------------------------- ITEO), LUNG ------- ---- (LU). ----- P-R-OS-T-AT-E----IP-R-).---SE-M-IN-A-L---VE-S-IC-L-E ---IS-V-I,---UR-E----- iLn 14 40 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON MADISON. LABORATORIES AMERICA. WISCONSIN U.S.A. INC. -------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67722 DATE OF DEATH: 02/12/91 SEX: 04ALE STUDY DAY OF DEATH: DOSE SS GROUP: 5 STUDY WEEK SACRIFICE STATUS: OF DEATH: I SCHEDULED SECOND INTE TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02/12/91 POST-FIX WEIGHER- NOT AVAILABLE 8:57 PROSECTOR: NANCY DIEDRICH RECORDER: DENNIS N ----------------------------------------------P-AT-H-O-LO-G-I-S-T:----D-R-. --T-O-M --P-AL-M-E-R ------------------WE-I-G-HE-R-:---P-A-TH-O-L-OG-Y-------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (ORAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN ---------------------BRAIN -------- -------- WEIOHT RATIO s -------- LIVER ILBRII 1.9111 25.0117 4609 % 1.0000 w LUNG LUI LF. TEITIS (TEO) 1.5412 1.6711 S:8210 % .3sto % .3888 % 12.0217 WE .7780 WE .843S WE RT. TESTIS TEIISO) ACCESS/SEX oia 1.6720 2.9S23 .3800 % .1440 WE --------------------------------T----------------------------------.-8-8-69---x ---------------1-.4-9-0-2--------------WE----- ORGAN NAME KEYWORDS. a oba P A T H 0 L 0 0 Y SEVERITY. DISTRIBUTION 0 0 S E R V A I 10 N S a a a GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI LIVER ILI) ------------------------------------------GROSS: - -A-L-L--T-I-SS-U-E-S/-O-R-GA-N-S----N-O-RM-A-L---------.--.--.--.-.- . . . . . . . . . . . . . . . . . . . . . ... .. .. . .. .. .. .. . .. .. . .. . .. MICRO: -MINIMAL, FOCAL -SLIGHT. FOCAL -MINI14AL -INF:iLiftATiS*M6NO'N'UCL'EA'R'CE'LL. . . . . . -NECROSIS COAGULATIVE -HYPEItTftOONY HEPATOCELLULAR: CHARACTERIZED CENTRILOBULATED PATTERN IN WHICH THE NEPAT A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 0 THE- FO-LLOW-ING - -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C ROUNDED GIVING T"IS_'EMLARGED' - EX-AMIN-ATI-ON.-- THE CELLS A MORE 'PLUMP- APP APPEARANCE, THE CELLS ARE 0 - -- --- --- BRAIN (13R). COAGULATING GLD (CO), LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE (SVI. URE - -------- ------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES . AMERICA. INC. MADISON. WISCONSIN U S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67723 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED. FIRST POST- DATE OF DEATH: 05/17/91 STUDY DAY OF DEATH: 149 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: DATE AND TIME OF NECROPSY: 05 17/91 8:51 PROSECTON: DEBBIE PIRKEL RECORDER: JOE M. KI P-O-S-T--F-I-X--W-E-IG-H-E-R-: --N-O-T--AV-A-I-L-AR-(-E----------------P-A-TH-O-L-OG-I-S-T:---D-R-.--T-O-M--PA-L-M-ER-----------------WE-I-GH-E-R-:--D-E-N-NI-S---HO-F--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 JORANSI TO BODY WEIGHT 1%) WEIGHT RATIO ---------------------- -------- -------- -------- s BRAIN LIVER IOLRII 2.1421 16.9011 .3118 % 3 1.0000 WE 7.1910 WE LUNG ILU) 1.66so :?9,1: %' .7774 WE LF. TESTIS OITEOIISOI 2.0423 .3639 % OS31 WE RT. TESTIS TE ACCESS/SEX 0 1.9492 3.2844 .3473 % SISI % .9097 WE I.S321 -----------------------------------------------------------------------------------------------W-E---------------- b ol6 P A T N 0 L 0 0 Y 0 8 S E R V A T 1 0 N S * A A O-R-GA-N---NA-M-E-------------K--E-Y-W-OR-D-S-. --S-E-V-ER-I-T-Y.---D-IS-T-R-IB-U-T-IO-N----------G--R--O--S-S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-H-OL-O-G-I------GENERAL COMMENT LIVER ILI) GAOWSI: -iLi itiS6Ei/6R6AkS'N6RkE PROSTATE M16ft6:'-iLiClhT'i.C)6Ai (PR) . . . . . . . . MICRO: -SLIGHT. @06Ai -INFILTRATES.MONONUCLEAR CELL -TiF@AAMiTiO@.*Chlt6NiC'A6TiVi . . . . . THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - BRAIN JONI. COAGULATING OLD (CO). LF. TESTIS (TEOI. LUNG (LUI. SEMINAL VESICLE (SVI, URETHRA (URI ---------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67724 SEX: MALE DOSE GROUP@ 5 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE Of DEATH: 02/13/91 STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I TERMINAL 6()DY WEIGHT: DATE AND TIME OF NEC70PSY: 02(13/91 7:32 POST-FIX WEIOHER: NO AVAILAO E PROSECTOR: JULIE A. LARSEN PATHOLOGIST: DR. TOM PALMER RECORDER: KEVIN BIL WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE OROAN TO BRAIN 0 ORGAN NAME ----------------- (GRANSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- s BRAIN 2.1162 4413 % 1.0000 WE LIVER 31.7272 6*.73S3 % IS.0256 WE LUNG LU) 1.5794 .334S % .7463 WE LF TEITIS TEO L.7s27 .3713 % .1202 WE IIT: TESTIS TEIISO, ACCESS/SEX 010 1.7111 3.2?OS .3024 % .0947 % .8086 WE I.S497 WE ------- ------------------------------------------------------------------------------------------------------ ORGAN NAME a 0 e PA T N 0 L 0 0 V 0 0 S E 4 V A T 1 0 N S 0 4 A KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI -------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER ILIJ 0400CSI:-ALE iliS6Ei/6R6AiS*N6RkAC 'ChAiA6TiNiZiD CENTRILOBULATED PATTERN IN WHICH THE NEPATO( A MORE HOMOGENEOUS CYTOPLASM AND THE CELL 8( ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPE THIS 'ENLARGED* APPEARANCE. THE CELLS ARE 0 THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - -------B-RAIN--(-O-N-)-.-C-O-A-G-UL-A-T-I-N-G --O-L-D --(C-O-)-.--LF-.--T-E-S-TI-S--(-T-E-O-).--L-U-N-G--(-L-U)-.--P-R-O-ST-A-T-E--I-P-R-).--S-E-MI-N-A-L--V-E-SI-C-L-E--(-S-V-I-,-U-P-E-T------------- tn 14 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7725 DATE Of DEATH: 01/23/91 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED FIRST INTERIM STUDY DAY OF DEATH: 35 DATE AND TIME OF NECROPSY: 01/23/91 9:08 STUDY WEEK OF DEATH: 5 TERMINAL 6ODY WEIGHT: POST-FIX WEIGHER: NOT AVAILABLE PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN ------------------------------------------P-AT-H-OL-O-G-IS-T-:---D-R-. --T-OM--P-A-L1-4-E-R---------------W-E-IG-H-E-R-: --G-A-IL--M-.--N-O-V--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ---------------------- -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- ST BRAIN a LIVER ILRIJ 1.9228 22.9038 .5548 % 1.0000 --WEI LUNG (LU) 1.3277 0.0011 % .383-1 % 11.9117 WEI( LF TESTIS ITTE RT'@ TESTIS Elol 1.7531 1.7120 Soso % .6905 .9117 WEI( WEI( AC6ESS/SEX 0 G (SO) 2.6365 .4939 % .7607 % .8904 WEIG -------------------------------------------------------------------------------1-.3-7-1-2------------W-E-IG---------------- O-R-GA-N---NA-M-E -------------K-EYWORDS. P A T H 0 L 0.0 Y SEVERITY. DISTRIBUTION 0 0 SE N VA T 10N S GROSS FREE-TEXT COMMENTS GENERAL COMMENT (Gc -----------------------------------------------------------------HI-S-T-OP-A-TH-O-L-OilG--I--C------ LIVER (LI) OROSI: -iL@ iliSU*Ei/6lt6AiS*N6Ri4A@ . M.IC.RO:. .-S.LI.GH.T . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -"YPERTPOP"Y HEPATOCILLULAR: CHARACTERIZED 0 CENTRILOBULATED PATTERN IN WHICH THE NEPATOCY A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BOR ROUNDED GIVINO THE CELLS A MORE 'PLUMP' APPEA THIS "ENLARGED' APPEARANCE. THE CELLS ARE OTH THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - -------B-R-A-I-N--(-BR-I-.--CO-A-G-U-L-AT-I-N-O--O-L-D--I-C-O)-.--L-F-.--TE-S-T-I-S--I-TI-O-)-.--L-UN-G--(-L-U-)-.-P-R-O-S-T-AT-E--(-P-R-I-,--SE-M-I-N-A-L -V-E-S-I-CL-E---(-SV-I-.--U-RE-T-H--------------- iLn "441- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67726 DATE Of DEATH: 01/23/91 SEX: 14ALE DOSE GROUP: 0 SACRIFICE STATUS: SCHEDULED FIRST INTERI STUDY DAY OF DEATH: 35 STUDY WEEK OF DEATH: 5 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 01/23/91 9:20 PROSECTOR: NANCY DIEDRICH RECORDER: JILL SEVA POST-FIX WEIGHER: NOT AVAILA13LE PATHOLOGIST: DR. TOM PALMER WEIGHER: (IAIL M. NO -------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 OR-GA-N---NA-M-E ---------- (GRAMS) TO BODY WEIGHT WEIGHT RATIO s -------- -------- -------- BIRAIN (841 L VER fLi LUNG ILU) 2.0124 16.4989 1.4624 .5418 % 4.4424 % .393-1 % 1.0000 WE 8.1016 WE .7267 WE L T STIS ITEOI 1.2929 .3481 % .6425 WE ATF@ TEESTIS El 1.3762 .370S % .0039 WE ACCESS/SEX 0 G Sol 2.4971 .6723 % 1.2409 WE ------- --------------------------------------------------------------------------------------------------- . % a a P A T 10 L 0,0 Y 0 8 S E R V A T 1 0 N S & & ORGAN NAME KEYWORDS. SEVERITY. DISTR" BUT16N GROSS FitEE-TEXT COMKHTS /a "ISTOPAT"OLOGI ----------------------------------------------------------------------------------------------7 ---- GENERAL COM14ENT GI(OICSI-:ALE itiS6Ei/6RdAiS*N6Ptl4A@ LIVER (LI) PROSTATE GENERAL IPRI . MICRO: -MODERATE. i4ULTi-iO@Ai INFORMATION IXXI . . . . . . . . . . . GRGSS: MiCiOOSi -'I'NF*LAM'MA*T*10*N.*C*"RO*N'IC*AC*TI*VE' iOiE': iAilkAi 6liD'Pil6it'T6iXiAiG6liA*16N' THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI!C-ROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - BRAIN IBR). COAGULATING OLD ICO), LF. TESTIS (TEO), LUNG (LU), SEMINAL VESICLE (SVI. URETHRA (URI ------------- ----------------------------------------------------------------------------------------------- 4Ln INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7727 DATE Of DEAIH@ 01/2S/91 SEX: MALE DOSE GROUP: a SACRIFICE STATUS: SCHEDULED FIRST INTERIO STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 8 TERMINAL AODY WEIGHT: DATE AND TIME OF NECROPSY: 01 25/91 7:09 PROSECTON: NANCY DIEDRICH RECORDER: JACK SERF( POST-FIX WEIGHER: NOT AVAILAO(I PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY I --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME JORANS) TO BODY WEIGHT WEIGHT RATIO s ---------------------- -------- -------- -------- BLAIRN 1L0;l L VE 2.0154 15.3142 SI22 % 3.94 371 x 1.0000 WE ?.SGIG WEI LUNG ILU) Lf TESTIS TEO 1.0491 1,6110 . 21i2 % .4142 X .8116 WEI .1993 WE RT@ TESTIS TEI 1,5435 .2960 % Ioso WEI ACCESS/SEX 010 Sol 2.1367 .7204 % 1.4075 WEI --------------------------------------------------------------------------------------------------------------- a a a P A T N 0 L 0'6 Y 0 0 S E R V A T 1 0 N S * 110 ORGAN NAME KEYWORDS.SEVERITY.DISTRIBUTION GROSS FREE-TEXT COM14ENTS / HISTOPAT"OLOGIC -------------------------------------------------------------------------------------------------------- PROSTATE lpit) . . . . . . . . . . . . . . . . . . . . . . . . . GROSS: -NODULAR -DiFOU@E@Y fttD'At@W'FiRif MICRO: -SLIGHT. FOCAL -INFLAMMATION, CHRONIC ACTIVE - -THE- FO-LLO-WINO- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - 13RAIN IBRI, COA(IULATING OLD ICOI. LF. TESTIS ITEOI. LIVER (LIJ, LUNG (LUI. SEMINAL VESICLE (SVI, URETHRA ---------------------------------------------------------------------------------------------------------------- ul APPENDIX 0 INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMALNUMBER: C$7722 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED. SECOND INTER DATE OF DEATH: 02/13/91 STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I TERMINAL BODY WEIGHT: DATE AND TIME OF NECROPSY: 02 13/91 7:30 PROSECTOR: DENNIS HOFFMAN RECORDER: KEVIN OIL POST-FIX WEIGHER: NOT AVAILAB(E PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMS I TO BODY WEIGHT 1%) WEIGHT RATIO s ---------------------- -------- -------- -------- BRAIN (ON) 2.OIS3 .4S03% 1.0000 WE LIVER ILIJ 16.4sts 3-7492 % $.LS33 WE LUNG (LU) 1.3873 .3162 % .6814 WE LF. TESTIS ITEO 1.6152 .3681 % .1015 WE RT. TESTIS ITEI i.oseo .3774 % .8217 WE ACCESS/SEX ORO isol 3.6721 .8369 % 1.1221 WE --------------------------------------------------------------------------------------------------------------- a* a PA TN0 L0 0 Y 08 S E RVA T10 NS a aA ORGAN NAME KEYWORDS.SEVERITY.DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI ------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER ILI) c Glaos.t:.-A.L@. i.ti.S6Ei/61tdAiS*N61thAi . M16NO: -SLIGHT, iO@Ai -liFiLtitATiS'.M6N6N6C@EAlt*CiL@ . . . . . ... THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON. - - - - - - - - - - - - BRAIN (SRI. COAGULATING OLD (CGI. LF. TESTIS (TEO). LUNG (LU). PROSTATE IPRI, SEMINAL VESICLE (SVI, URE --------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67729 DATE OF DEATH: 02/14 91 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, SECOND INTERI STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 TERMINAL BODY WEIGHT: DATE AND TIME OF NEC40PSY: 02(14/91 1:17 PROSECTOR: NUT" SCHNITZ RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAR E PAT14OLOGIST: DR. TOM PALMER WEIGHER: VAL QUINN ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRANSI TO BODY WEIGHT (1) WEIGHT RATIO ST ---------------------- -------- -------- -------- ORIA N 2.803:S9 .4425 % 1.0000 WEI L VC'R ILO!L 19. 3 4.3os4 % 9.7305 WEI LUNG (LU) 1.4861 .3227 % .7292 WEI LF TESTIS TE 1,7331 .3761 % .1500 WEI NT: TESTIS TEO 1.6801 .3640 % .8240 WEI ACCESS/SEX 010 ISO) 3.1071 .0743 % 1.5239 WEI ---------------------------------------------------------------------------------------------------------------- s a a P A T " 0 L 0-0 Y 0 0 S E R V A T 1 0 N S h A 0 ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPAT"OLOGIC -------------------------------------------------------------------------------------------------------- GENERAL COMMENT 0400CSI:-iL@ itiS6Ei/6*dAiS'N6Ni4Ai * * * ' * * . . . . . . - -THE- FO-LLOW-ING- -TISS-UES -WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - - BRAIN SRI COAGULATING OLD (COI. LF. TESTIS (TEO). LIVER ILI). LUNG (LU), PROSTATE (PRI. SEMINAL VESICL UREIHRI (Uhl ---------------------------------------------------------------------------------------------------------------- cc INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?730 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED TERMINAL SACR DATE OF DEATH: 03425/61 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: DATE AND TIME OF ECROPSY: 03(25/91 9:33 PROSECTOR: JACK SERFORT PATHOLOGIST: OR. TOM PAL14ER RECORDER: JOE N. KIZ WEIGHER: STEVE VAN A P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-OT--A-V-A-IL-A-S--E-------------------------------------------------------------------------------------- ABSOLUTE ORGAN W[IOMT ORGAN WEIGHT RELATIVE OROAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIOHT -------- WEI-GH-T---R-AT-I-O ST --- BRAIN (OR) LIVER ILI) 2.0387 16.3SOI .4100 % 3.3342 % 1.0000 WEI 1.0202 WEI LUNG (LU) LF. TESTIS ITEO) l.so3i 1.6753 .32S'l % .2411 % .7814 WEI .4217 WEI RY. TESTIS (TEI 1.6759 .3420 % .1220 WEI ACCESS/SEX ONG ISO) 3.3666 - .- .6869 % l.6sl3 WEI - --- - ----- - -.---------- ----------------- ------------------------------------- ---P-- -A- -T N 0 L 0*0 Y 0 8 S E R V A T 1 0 N S * * * O-R-O-A-N--N-A-M-E------------K-E-Y-W-O-R-D-S-.-S-E-V-E-R-I-T-Y-.-D-I-S-T-R-I-B-U-T-I-O-N-------G--R-O--S-S---F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-M-OL-O-G-IC-------- GENERAL COMMENT - - - - - - -04(-OIC-SI-:-iLi -il@-S6E-i/6-R4A-iS'H-6Rk-@ - - - - - - - - - - - - - - - - - - - - - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: B:AIN,,IBII,,COAOULATING OLD (CO). LF. TESTIS (TEO). LIVER ILII. LUNG ILU). PROSTATE (PRI. SEMINAL VESICL -------U -I----A--U------------------------------------------------------------------------------------------------- 4 so INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CGI?3t DATE Of DEATH: 02/12/91 SEX: MALE DOSE OROUP: 0 SACRIFICE STATUS: SCHEDULED SECOND INTERI STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: DATE AND TIME Of NECROPSY: 22(12/91 7:20 PROSECTON: NANCY DIEDRICH RECORDER: DENNIS HOF P-O-S-T--F-I-X--W-E-IO-"-E-N:---N-OT--A-V-A-IL---8-E----------------P-AT-H-O-LO--G--I--ST--:-----D-R-.--T-OM--P-A-L-ME-R----------------W-E-IG-H-E-R:---P-A-T-HO-L-OG-Y---D-------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (ORAMS) -------- TO BODY WEIGHT -------- WEIOHT RATIO -------- ST --- BRAIN ILBRI 2.0238 .4SI2 % 1.0000 WET LIVE 1 16.1915 3.6101 % s.ooos WEI LUNG ILUI 1.6026 .3S?3 % .7919 WEI LF TESTIS (TEOti RY.* TESTIS IIE 1.7209 1.7494 .3837 % .3001 % IS03 WEI .8644 WEI ACCESS/SEX ORO ISOI 3.SI67 .7841 % 1.7377 WEI --------------------------------------------------------------------------------------------------------------- a a a P A I H 0 L 0'0 Y 0 0 S E P V A T 1 0 N S * #I& O-R-G-AN---NA-M-E --------------K-E-Y-WO-R-D-S,---S-E-VE-R-I-TY-,---DI-S-T-RI-B-U-TI-O-N----------O--R--O-S-$---FR-E-E--T-E-X-T --C-O-M--M-EN-T-S---/---H-I-ST-O-P-AT-H-O-LO-G-IC GENERAL COMMENT G4(OICSI-:iL@ iliS6[i/6R6AiS'N6RkAi . . .. . ... . URETHRA (URI A16R6:'-iE6TiOi iXiMiNiD;' ilS@E'N6T'PiEiEiT* - -THE- FO-LLOW-INO- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON.-- - - - - - - - - - - - - - BRAIN (891, COAGULATING OLD (CGI, Lf. TESTIS (TEOI, LIVER (LI), LUNG (LUI, PROSTATE (PRI. SEMINAL VESICL --------------------------------------------------------------------------------------------------- cn 0C20 APPENDIX D INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C07732 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED TERMINAL SACRI DATE OF DEAT14: 03/26/91 STUDY DAY OF DEATH: 97 STUDY WEEK OF DEATH: 14 TERMINAL ADDY WEIGHT: 5 DATE AND II14E OF NECROPSY: 03 26/91 7:S2 PitOSECTON: DEBBIE PIRKEL RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILAB(E PATHOLOGIST: ON. TOM PALMER WEIGHER: JOE H. KIZZI ----------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0A O-R-G-AN---NA-M--------------- (GRAMS) -------- TO BODY WEIGHT 1%) WEIGHT RATIO -------- S--T-- BRAIN (OR) 2.2330 .4107 % 1.0000 WEIG LIVER ILI) 15.0333 2.937S % 6.9991 WEIG LUNG ILU) l.s7s6 .2961 % .1054 WEIG LF. TESTTS OITEO 2,0543 .3160 % .9197 WEIO NT. TESTIS YEI 1.9868 .3733 % .889S WEIG ACCESS/SEX (I isol 2.2441 .4218 % 1.0050 WEIG ----------------------------------------------------------------------------------------------------------------- 0 a * P A T H 0 L 0,0 Y 0 8 S E 0 V A T 1 0 N S A a * ORGAN---N-AM-E--------------K-E-Y-WO-R-D-S,---S-E-VE-R-I-TY-,---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T --C-O-M-14-E-N-TS---/---HI-S-T-OP-A-T-HO-L-7O-G-I-C---- GENERAL COMMENT (Il'o'si-:AL@ ittSOEi/6R6AhS'N61tkAi . . . . - -TH-EF-OLL-OW-IN-TGIS-SU-EWS-ERE-UN-RE-MA-RKA-BAL-TEM-ICR-OS-CO-PEI-XCAM-IN-AT-IO-N:- - - - - - - - - - - - - BRAIN On) COAGULATIONLGD(CO).LF.TESTISITEO).LIVERILI).LUNG(LU).PROSTATIEPRI.SEMINAVLESICLE UNET"nl (Uol -----------------M----m ------------------------------------------------------------------------ APPENDIX 0 INDIVIDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL"UMBER: C67733 SEX: 14ALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED FIRST INTERIP DAIE OF DEATH: 01/23/91 STUDY DAY OF DEATH: 35 STUDY WEEK OF DEATH: 5 PROSIECTOR@ NANCY DIEDRICH TERMINAL IODY WEIGHT: RECORDER: JILL SEVAP DATE ANI) 71HE OF NECROPSV; 01/23/91 7:33 PATHOLOGIST: DR. TOM PALMER WEIGHER: OAIL M. NO P-O-S-T---F-I-X----W-E-I-G-H-E-R-:---N-O-T---A-V-A-I-L-A-B-L-E----------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ----------------- IGRANS) -------- TO BOOV WEIGHT (%I -------- WEI(INT RATIO -------- s BIRAIRN 1L8RIJ 1.0316 .4769% 1.0000 WE L VE LUNG ILU) LF. TESTIS ITEl0 16.727S I.S339 1.7651 4.3sso % .3993 % .4SOS % 9.1327 WE .03?S WE .9637 WE R7. TESTIS IE 1.7714 1.8099 .4612 % .4191 % .9471 WE .8790 WE ACCESS/SEX ORO ISO) ------------------------------------------------------------------------------------ A a * P A irH o L 6 a v 0 8 S E R V A T 1 0 N S a & a OROAN NAME KEYWORDS. SEVfnllY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI ------------------------------------------- ------------------------------------------------------------- GENERAL COMMENT GIGOCSI:-AL@ iliS6[i/bft6AkS'NOItkA@ .. .. .. . .. .. . .. . LIVER ILI) ilT6R6:"-iLiGhT' F'&A@ SLIGHT: DIFFUSE C'OA'G'UL'AT'IVE' -FATTY CliANGE TH-EF-OL-LOW-IN-TGIS-SU-EWS-ERE-UN-RE-MA-RKA-BAL-TEM-ICR-OS-CO-PEI-XCAM-IN-AT-IO-N:- BRAINIBRI.COAGULATIONLGD ICO),LF.TESTIS(TEO).LUNGILUI,PROSTAT(EPRI,SEMINAVLESICL(ESVI,URE ---------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- AllIKAL 14UMBER: C67734 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE OF DEATH: 02/12/01 STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02/12/91 8;35 PROSECTOR: KEVIN BILLINOS RECORDER: DENNIS 140 POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY -------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIOHT ORGAN WEIGHT RELATIVE ORGAN TO GRAIN 0 O-R-GA-N---NA-M-E -------------- (GRAMSI -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- s BRAIN JORI 2.0170 .4907 % 1.0000 WE L VER Li ls.sess 3.7312 % 7.4961 WE LUNG (LU) 1.4530 .3409 % .0996 WE LF. TESTIS 177EEOl l.s371 .3691 % .7401 WE RT. TESTIS 1.5238 .3659 % .7337 WE -------AC-C-ES-S-/-SE-X---0-1-0---IS-O-)-----------------3-.-0-4-02-------------------.-7-2-99--%--------------1-.-4-6-37-------------WE----------- .1a a P A T H 0 L 0 0 Y 0 8 S E R V A T 1 0 N S * a ik O-R-GA-N--N-A-M-E -------------K--E-Y-WO-R-D-S.---S-E-VE-R-I-TY-,---DI-S-T-RI-B-U-TI-O-N----------O--R-O--S-S---FR-E-E--T-E-X-T --C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-HO-L-O-OT------ GENERAL COMMENT GIGOCSI:-ALi fliS6Ei/dRdAiS'N6RkA*L - --- ------ -- ---- --- --- --- -- ---- --- --THEFOLLOWINTOISSUEWSEREUNREMARKABALTEMICROSCOPEIXCAMINATION: BRAINHltl"(lU1, COAGULATING OLD ICOI. LF. TESTIS ITEO). LIVER (LIJ, LUNG (LUP. PROSTATE (PRI, SEMINAL VESIC --------U RE-T--------I -------------------------------------------------------------------------------------------- 0q-0, INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA MAZLETO" LABORATORIES AMERICA. INC. MADISON. WISCO"SIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C6773S SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED. FIRST INTERI STUDY WEEK Of DEATH: 6 TERMINAL BODY WEIGHT: DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 36 DATE AND TIME OF NECROPSY: 01/24/91 ?:S3 PROSECTOR: DENNIS HOFFMAN RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: DEBBIE PIR --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- s ---------------------- BRAIN IBRI 1.9243 17.0568 sois % 4.44s3 % 1.0000 WE 8.1639 WE LIV LUNG I.S482 .403S % .8046 WE LF. TELLTUII ITEO I.SI07 .3931 % .?&St WE RT TESTIS ITEII 1,4701 2.2190 .3131 % S?93 % .7640 WE t.ts3i WE -------AC-@-E-SS-/-S-E-X--0-0(-i--I-S-O-)---------------------------------------------------------------------------------------- a6 a PA IN0 L0 0 Y 0 9S tN V AT 10 NS AA 6 ORGAN NAME K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---D-IS-T-R-IB-U-T-IO-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-I - ---------------------- GENERAL COMMENT GIGOCSI:-iLi iliS6Ei/6R6AiS'H6Rki .. .. . . LIVER ILI) . Al@R6: '-AI'Nli4A'L.'F6Ci.L . . . . . . . . . 'liFiLiltATiS'M6N6N6CiEAft*CiL@ . . . . - - ---THE FOLLOWING - -- TISSUES WERE --- --- --UNNE14ARKABLE AT MICROSCOPIC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAIN (BRI, COAGULATING OLD ICU). LF. TESTIS ITEO). LUNG ILU). PROSTATE (PRI. SEMINAL VESICLE (SV), URE -------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------I------------------------------------------------------------------------------------ ANIMAL NUMBER: C$7736 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED TERMINAL SACR DATE Of DEATH: 03/26/91 STUDY DAY or DEATH: 27 STUDY WEEK OF DEATH: 14 TERMINAL IODY WEIGHT: DATE AND TIME OF NECROPSY: 03/26/91 9:27 PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN POST-FIX WEIG14ER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: JOE N. KIZZ ---------------------------------------------------------------------------------------------------------------- ABSOLUTE 00(IAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- ST --- BIRAIN JORI 2.1141 .4093 2 1.0000 WEI L VIA Ll ls.8731 3.0733 % 7.501S WEI LUNG ILU) 1.470S .2141 % .4956 WEI Lf. TESTIS ITE 1.7649 .3411 % .1348 WEI AT. TESTIS (TE?l 1.73so .3361 X .8211 WEI ACCESS/SEX ORO Sol 3.2314 .6270 % l.s3lo WEI ---------------------------------------------------------------------------------------------------------------- I * * * P A I N 0 L 0.0 V 0 0 S E P V A T 1 0 N S A * & O-R-OA-N---NA-M-E-------------K--E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S--S --F-RE-E---TE-X-T--C-O-M-ME-N-T-S---/---"-IS-T-O-PA-T-H-OL--O-G--I-C----- GENERAL COMWNT LIVER ILI) 04'.OCS.I:.-A.L@. f.li.S6.Ei/.6R.dA.iS*.N6.RI.@A@. . . . . . . . . AICRO: -SLIO"T. FOCAL -1'"FiLiRATiS'.M*ON'OMt')C*LEA'R'CE*L'L THE- FO-LLOW-ING- -TISS-UES- WE-RE -U"R-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EXAMINATION:- - - - - - - - - - - - - - BRAIN (BRI. COAGULATINO OLD ICOI. Lf. TESTIS FTEOI. LUNG ILUI. PROSTATE IPR). SEMINAL VESICLE (SVI, URET ------------------------------------------------------------------------------------- ---------------- tn co o.n INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. MADISON, WISCONSIN U.S.A. INC. ---------- -------------------------------------------m-------------------- ------------------------------- ANIMAL NUMBER: CB?737 DATE Of DEATH:002F412/91 SEX: MALE DOSE GROUP: 8 SACRIFICE STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: PROSECTOR: RUTH SCHMITZ STATUS: I SCHEDULED, SECOND INTERI TERMINAL BODY WEIGHT: RECORDER: DENNIS WF DATE AND TIME ECROPSY: 02 12/91 8:47 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY D -------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 O-R-G-AN---N-AM-E-------------- --IG-N-AM-S-I-- 70 BOD-Y --W-E-IG-H-T- WEI-GH-T---R-A-TI-O S T BRAIN Lli 4700 % 3.4S4? % 1.0000 WEI 7.3SO2 WEI LIVE LUNG ILUI IS.9011 1.6sto .35*S % .7626 WEI LF E TEO 1.5674 .3404 % .329S % .7242 WEI .7010 WEI OIT@ 1TESSTT'ISS TEI I.SI?3 3.1873 .6921 1 1.4726 WEI -------A-CC-E-S-S/-S-EX----0-1-0----is-o-i-------------------------------------------------------------------------------------- . a a a P A T " 0 L Q.0 V 0 8 S E R V A T 1 0 N S f,0 * ORGAN NAME KEYWORDS, SEVERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC -------------------------------------- ---------------------- --------------------------------------- GENERAL COMMENT GA(OICSI:-ALi iliS6Ei/61tdAiS*N6*AA@ - -- - --- ----- --- -- ---- -- -- --- --- --- ---- THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN BRI COAGULATING OLD (CO). LF. TESTIS (TEO), LIVER ILI). LUNO ILU). PROSTATE IPR). SEMINAL VESICL --------U-R-ET-H-R-I ---J-UA-I------------------------------------------------------------------------------------------- oil co at INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG??34 SEX: MALE DOSE GROUPI 6 SACRIFICE STATUS: SCHEDULED TERMINAL SACR DATE OF DEATH: 03/2S/91 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 03/2S/91 0:42 PROSECTOR: JACK SERFORT RECORDER: JOE M. KIZ POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: STEVE VAN A ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- ((IftAMS1 -------- 70 BODY WEIGHT (Zi -------- WEIGHT RATIO -------- ST 61AIIaM 1L9IJ 2,1308 17,0900 .4091 % 3.2672 % 1.0000 WET 7.9871 WEI L VE LUNG ILU) 1.6216 .3112 % .7606 WEI LF. TESTIS ITEO) 1.9sos .3729 % oils WEI NY IESTIS IIEII 1.?333 .3314 % .1100 WEI AC@ESS/SEX ORO (Sol 3.2127 .6142 Z I.SO14 WEI ---------------------------------------------------------------------------------------------------------------- 00 & PA TH0 L0 0 Y 08 SE NVA T10 NS A 00 O-R-GA-N---NA-M-E -------------K--E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S- FREE-TEXT COMMENTS / HISTOPAT"OLOGIC --------------------------------------- GENERAL COMMENT A'oacsl-:AL@ iliS6Ei/61tdAiS'N6ltkA@ . .. .. . LIVER ILI) . i4ldR6:*-AIAIAAi.'F6CAL** * ' ' * . . . . . . . .liFiLiltATiS*.M6N6006C@EAR*CiLL -- -- -- ----- -- ----- --- -- -- ---- -- --- --- - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (OR), COAGULATING OLD ICGI. LF. TESTIS (TEOI. LUNG (LU), PROSTATE (PRI. SEMINAL VESICLE (SVI. URET --------------------------------------------------------------------------------------------------------------- us INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA MAZLEYON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?739 DATE Of DEATH: 02/14/21 SEX: 14ALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED SECOND INTERI STUDY DAY OF DEATH: Sl STUDY WEEK Of DEATH: 9 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02/14/91 7:13 PROSECTON: KEVIN BILLINGS RECORDER: JILL BEVAP POS7-FIX WEIGHER: N07 AVAILABLE PATHOLOGIST: DR. 7014 PALMER WEIGHER-. VAL QUINN -------------------------------------------------------------------------------------------------- ----------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (GRANSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- ST --- BRAIN (Bit) 2.1329 .4503 % 1.0000 WEI LIVER ILI) le.osso 3.43SO% I.S2?3 WEI LUNG ILU) 1.40as .3009 % OS94 WEI LF TES71S TEO1 1.4698 .314S % .6191 WEI NT: TESTIS TE 1.4416 .3084 % .67s9 WEI ACCESS/SIEX JO ISO) 2.0103 .4301 % .942S WEI ------------------------------:---------------------------------------------------------------------------------- & & e P A I N 0 L 0-0 V 0 9 S E N V A I 1 0 N S & 4 0 ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-7EXT COMNENTS / "ISTOPATHOLOGIC -------------------------------------------------------------------------------------------------------- GENERAL COMMENT c GA(olsl:-ALi iliS6iEi/6N6AiS'116R#@Ai THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AY- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - 13RAIN JOR), COAGULATING GLD (CO), LF. TESTIS (TEO). LIVER ILI), LUNG ILUI, PROSTATE (PRI. SEMINAL VESICL UREIHW JUR) -------- ----------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLE70N LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: CS?740 SEX: MALE DOSE GROUP. 6 SACRIFICE STATUS: SCHEDULED TERMINAL SACR DATE OF DEATH: 03/22/91 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEISfAt-. DATE AND TIME OF NECRO:SY: 03/22/91 0:29 PROSECTON: RUTH SCHNITZ RECORDER: JOE N. KIZ POST-FIX WEIGHER: N07 VAILABLE PATHOLOGIST: ON. TOM PALMER WEIGHER: JILL BEVAN ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMS) -------- TO BODY WEIGHT (1) -------- WEIGHT RATIO -------- ST BRAIN (6R) 2.1000 .4500% 1.0000 WEI LIVER ILI) IS.2SSS 3.251* % 7.2645 WEI LUNG ILU) 1.3691 .2934 % GS20 WEI LF TESTIS TEO11 1,6609 .3559 % .7909 WEI RT: TESTIS TE 1.6390 .3S14 % .7800 WEI ACCESS/SEX 010 ISO) 3,096S .663S % 1.474S WEI ---------------------------------------------------------------------------------------------------------------- 6 a 6 P A 'fN 0 L 0 0 Y 0 8 S E N V A I 1 0 H S & A A O-R-G-AN---NA-M-E-------------K-E-Y-WO-R-D-S,---S-E-VE-R-I-TY-.---D-IS-T-R-IB-U-T-IO-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-O-PA-T-"-OL-O-A-IC-------- GENERAL COMMENT GI(OICSI-:iLi iliS6Ei/6lt6AiS*146RiiAi - --- --- -- --- - - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:BRAIN ON) COAOULATINO OLD ICO). LF. TESTIS (TEO). LIVER (LIJ, LUNG (LUI, PROSTATE (PRI, SEMINAL VESICL -------u-----I--t-U-A-)---------------------------------------------------------------------------------------------- co U2 I"OIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. MADISON, WISCONSIN U,S.A. INC. -------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CS?741 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED FIRST INTENT DATE OF DEATH: 01/23 1 STUDY DAY OF DEATH: 3S STUDY WEEK OF DEATH: 5 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NEC49OPSY: 0)(23/91 7:30 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL BEVA POST-FIX WEIG"ER@ NOT AVAILAO E PATHOLOGIST: DR. TOM PALMER WEIGHER; GAIL M. NO ------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMSI TO BODY WEIGHT 1%) WEIGHT RATIO s ---------------------- -------- -------- -------- ORIA 8 1.9498 .4955 % 1.0000 WE L VEIIN IL71 15.6120 3.96?S X 8.0070 WE LUNG fLU) 1.5300 .3118 % .7847 WE LF TESTIS TEOISOP l@6SS7 .4208 x .8492 WE FtT: TESTIS TEI 1.7416 .4426 % .0932 WE ACCESS/SEX OIG 2.2005 .7117 % 1.4363 WE --------------------------------------------------------------------------------------------------------------- A a 0 P A T N 0 L 0 0 V 0 0 S E R V A T 10 N S A * A ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / "ISTOPAT"OLOGI( ---------------------- ----------------------------------------------------------------------------------- GENERAL COMP4ENT 01'03CSI-:ALi iliS6E/61tdAiS'N6ltAAi - -THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATIO-N: - - - - - - - - - - - - - - BRAIN ON). COAGULATING OLD ICO), LF. TESTIS FTEO). LIVER (LI), LUNG (LUI, PROSTATE IPRI. SEMINAL VESICL UNETHRI (URI --------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLE70H LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- A"lt4AL "UMBER: C$7742 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST INTERI DATE OF DEATH: 01/24/91 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: DATE AND 71ME OF NECROPSY; 01/24/91 7:OS PROSECTON; DENNIS HOFFMAN RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST; ON. VON PALMER WEIGHER: DEBBIE PIN --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMS) -------- 10 BODY WEIGHT 1%1 ------ WEIGHT RATIO -------- s BRAI 1.9377 .5020 % 1.0000 WE LIVERN leLrl 10.1264 4.1778 % 1.3224 WE LUNG ILU) 1.2800 .3310 % .6606 WE LF TESTIS RT@ TESTIS TEO) 7EI)SO) 1,6971 1.6902 .4397 % .4379 % .8758 WE .8723 WE -------AC-C-ES-S-/-SE-X---0-1-0-------------------2-.-81-1-1-------------------.-7-4-40--%--------------1-.-4-12-1-------------WE--------------- a obe P A T H 0 L 0.0 Y 0 0 S E P V A T 1 0 N S lia a ORGAN NA14E KEYWORDS. SIEVERITY._DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI ------------------------------------------------------------------------------------------------------ KIDNEVIS) IKD) . . . . . . . . . . . . . . . . . . . . . . . . . . . GROSS: -LARGE PELVIS(ES) MICRO: -PRESENT -RIGHT: iOtH'C6LiE6TiD* -DILATATION.PELVIC . . .. . ... THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON-- - - - - BRAIN (OR), COAGULATING GLD JCGI. LF. TESTIS (TEO). LIVER ILI). LUNG ILUI. PROSTATE IPRI, SEMINAL VESIC URETHRA JUR) --------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- AN114AL NUM13ER: CO?743 DATE OF DEATH: 02/13/91 DATE AND T114E OF NECROPSY: SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: STUDY DAY OF DEATH; SO STUDY WEEK OF DEATH: I 02(13/91 7:17 PROSECTOR: JULIE A. LARSEN SCHEDULED SECOND INTERI TERMINAL IOOY WEIGHT: RECORDER: KEVIN BILL POST-FIX WEIGHER: NOT AVAILAS E PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIG"T RELATIVE TO BODY WEIGHT ORGAN TO BRAIN WEIGHT RATIO 0 ST ORGAN NAME ---------------------- -(-G-R-A-MS-)-- -------- -------- --- BRAIN JORI 2.2013 17.6532 SIS2 % 4.i2ii% 1.0000 WEI 7.$061 WEI L VER Ll LUNG (LU) 1.6951 .8 2 % .7496 WEI LF. TESTIS ITEOI 1.6251 .3703 % .3413 % .7181 WEI .6761 WEI PT. TESTIS (TEL ACCESS/SEX ORO ISO) I.S2$9 2.2809 S211 % 1.0113 WEI --------------------------------------------------------------------------------------------------------------- a * a P A 7 N 0 L 0 0 Y 0 IDS E R V A T 1 0 N S A * & ORGAN NAME K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-O-PA-T-H-OL-O-O-I(-------- ---------------------- ... . . . . . GENERAL COMMENT GI(OICSI-SA:Li iliS6E@/6lt6AiS*"6ltOAi . .. . .. LIVER (LI) MICRO: . -SLIGHT. AU@Ti-iC@Ai ... . ... . . . . . . .. ... . - - -THE- FO-LLO-WING- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MICROSCOPIC - EX-AMIN-ATI-ON.-- - - - - - - - - - - - - - BRAIN (BRI, COAGULATING GLD ICGI. LF. TESTIS ITEOP, LUNG ILU), PROSTATE IPR). SEMINAL VESICLE (SVI. URE ------------------------------------------------------------------------------------------------------ to INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$1744 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED TERMINAL SACA DATE Of DEATH: 03/26/91 STUDY DAY OF DEATH: 97 STUDY WEEK or DEATH: 14 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 03(26/91 8:51 PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVAN POSI-FIX WEIGHER: NOT AVAILAS E PATHOLOGIST: OR. TOM PALMER WEIGHER: JOE N, KIZZ ------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN S0 I ORGAN NAME - --------------- (GRAMSI -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO -------- ... BRAI 2.2132 .4394 % 1.0000 WEI LIVER" IOLII IS.SS20 3.2920 % 1.4923 WEI LUNG (LU) 1.5768 .3130 % .7125 WEI Lf TESIIS If 1.6972 .3399 % .7662 WEI OT@ TESTIS TEI 1.7381 .3451 % .7153 WEI ------A-C-CE-S-S-/S-E-X---0-10--O-I-S-O-) --------------4-,6-7-7-4-------------------.-92-1-6--% --------------2-.-1--1--3-4--------------------W-EI--- P A I N 0 L0 0 Y 0 0 S f A V A T 10 N S A A A OR-GA-N---NA-M-E-------------K-E-Y-WO-R-D-S.---;-E-;E-l-&-tI-T-Y-. -DI-S-T-RI-B-U-TI-O-N----------G--R-O--S-S-- FREE-TUXT COMMENTS / HISTOPATHOLOGIC ----------------------------!------ GENERAL COMMENT 040OCst:-iLi il@S6Ei/6NdAiS'N6Ri4Ai * * ' * ' ' * . . . . . . --- ----- -- ---- --- --- -- --- --- -- --- --- THE FOLLOWING TISSUES WERE UMBE14ARKABLE AT MICROSCOPIC EXAMINATION: BRRAIN COAOULATING OLD (CO), Lf. TESTIS (TEO), LIVER (LI). LUNG (LU). PROSTATE IPRI, SEMINAL VESICL -------u--E-l-----JU-R-)----------------------------------------------------------------------------------------------- 4.n 44-01 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CO?74S SEX: 14ALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST INTENT14 DATE OF DEATH: 01/23/91 STUDY DAY OF DEATH: 35 STUDY WEEK OF DEATH: S TERMINAL BODY WEIGHT: 3 DATE AND TIME OF NECROPSY: 01/23/91 9:07 PROSECTON: DENNIS HOFFMAN RECORDER: JILL BEVAN POST-FIX WEIG14EN: NOT AVAILABLE PATHOLOGIST: ON. TOM PALMER WEIGHER: GAIL M. NOVA ----------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- (GRAMSI -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0R sT ---- BRAIN 0 2.0090 .5073 % 1.0000 WEIG LIVER ILIRI IS.1591 3.8282 % 7.5459 WEIG LUNG ILU) I.SI59 .3128 % .7s46 WEIG LF. TESTIS E 1.7783 .4491 % .8152 WEIG NT. TESTIS ITTEOIJ 1.7371 .4318 % .1650 WEIG ACCESS/SEX 0 G SO) 2.4131 .6094 % 1.2011 WEIG ----------------------------------------------------------------------------------------------------------------- e 10a P A T H 0 L 0 0 Y 0 0 S E N V A T 1 0 M S a k a ORGAN MAHE KEYWORDS. SEVERITY, DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLO(IIC ---------------------------------------------------------------------------------------------------------- GENERAL COMMENT c 04(olst:-iLi iliS6Ei/61tdAkS'N6RkAi . .. .. . THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMIN-ATI-ON:- - - - - - - BRAINHRIBN I ),,COAGULATING OLD ICO). LF. TESTIS (TEOP, LIVER (LIJ, LUNG (LU). PROSTATE (PR). SEMINAL VESICLE URET u ----------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLE70H LABORATORIES AMERICA, INC. MADISON. WISCONSIN U S A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67746 SEX: MALE DOSE GROUP: 0 SACRIFICE STATUS: SCHEDULED TERMINAL SAC DATE OF DEATH: 03/2S/91 STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL 600Y WEIGHT: DATE AND TIME OF NECROPSY: 03/2S/01 8:27 PROSECTON: JACK SERFORT RECORDER: JOE N. KI POST-FIX WEIGHER: 1#07 AVAILABLE PAT14OLOGIST: DR. TOM PALMER WEIG14ER: STEVE VA" --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN 10 BRAIN 0 ORGAN NAME (GRAMS) TO BODY WEIGHT IZI WEIGHT RATIO s ---------------------- -------- -------- -------- BRAIN 1L8li LIVER 2.1138 14.3227 .4456 % 3.0196 % 1,0000 WE 6.7764 WE LUM 0 JLU11 1.7241 .3637 % .8160 WE LF. IE T ITEOI 1.6021 .3376 % .7sto WE RT. TESTIS ITEI 1.576$ .3324 % .7460 WE ACCESS/SEX ONG ISOI 3.4218 .7229 % 1.6223 WE --------------------------------------------------------------------------------------------------------------- a a * P A T H 0 L O.G Y 0 6 S E N V A T 1 0 N S 110 A ORGAN NA14E KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI -------------------------------------------------------------------------------------------------------- GENERAL COMMENT 4GCI: G OSS -iLi il@S6E/6RdAkS'146Ri4Ai LIVER (LI) -INFILTRATES.MONONUCLEAR CELL THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN (OR). COAGULATING OLD (COO, LF. TESTIS (TEO), LUNG (LUI. PROSTATE FPRI, SEMINAL VESICLE (SV). URE --------------------------------------------------------------------------------------------------------------- to C" INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S A. ---------------------------------------------------------------------------------------------------------------- ANT14AL NUMBER: C67747 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED. TERMINAL SACR STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: DATE OF DEATH: 03/26/91 STUDY DAY OF DEATH: 97 DATE AND 71ME OF NECROPSY: 03 26/91 8:19 PROSECTOR: STEVE VAN ADESTIME RECORDER: JILL BEVAP POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: Dt. 70H PAL14EN WEIGHER: JOE H. KIZ --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMS) -------- TO BODY WEIGHT 1%1 -------- WEIGHT RATIO -------- s ---------------------- BRIA 00 2.1360 20.1188 .3932 % 3.19749 3 11 1.0000 WE 9.1704 WE L VE1R" ILIJ LLFUNG TESITLIUS) TEOI 1.4971 1.6121 . % .300S % .1012 WE IS47 WE FIT: TESTIS TE I.S406 .2872 % .?213 WE ACCESS/SEX 010 isoi 3.5351 GS*2 % 1.6sss WE ------- -------------------------------------------- ---------------------------------;---;-,-;---P--A--7-H--0--L--0-70- V 0 8 S E R V A T 1 0 N S A & A ORGAN NAME K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---HI-S-T-O-PA-T-"-OL-O-G-I------- ---------------------- GENERAL GENERAL COMMENT 04(OICSI:-AL*L iliS6Et/6R6AkS'N6ltl4A@ INFORMATION (XX) . . . . . . . . . . . . . . . . . . . . . . . . . . . GROSS: NECROPSY NOTE: )ANIMAL DIED PRIOR TO iX@Ai(16liAil6N'.iL6()6 THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATION: 1,,COAGULATING GLD (CO), LF. TESTIS (TEOI, LIVER (LIJ, LUNG (LU), PROSTATE IPRI, SEMINAL VESIC OURRAEITHNRIBI(tu -------------------------------------------------------------- ------------------------------------------------ to 0% INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC, MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67741 DATE OF DEATH: 03/22/91 SEX: MALE DOSE GROUP: a SACRIFICE STATUS: STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH@ 14 22/91 9:00 PROSECTOR: KEVIN BILLINGS SCHEDULED TERMINAL SACR TERMINAL 600Y WEIGHT: RECORDER: JOE W. KIZ DATE AND TIME OF NECROPSY: 03 POST-FIX WEIGHER: NOT AVAILAO(E PATHOLOGIST: DR. TOM PALMER WEIGHER; JILL BEVAN ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NA14E JORAMSI -------- TO BODY WEIG"T 1%) -------- WEIGHT RATIO -------- ST --- ---------------------- SIAIN IBRI 2.1320 .4235 % 1.0000 WEI L VER Ll is.gos? 3.17?S % I.SO27 WEI LUNG (LU) L TI TE IR'T: T'EESSTISS T EIO 1,62S2 1.8997 1.7396 .322$ % .337$ % .3456 % .7623 WEI .7972 WEI IIS9 WEI ACCESS/SEX 01 (1 ISO) 3.7221-------------------.-7-32-4--%--------------1-.-74-5-$------------W-E-I--------- ------------------------------------------- a e ft P A 7 H 0 L 0 0 V 0 8 S E R V A T 1 0 N S a * A ORGAN NAME KEYWORDS. SEVERITY. DISTRI13UTION G-R-O-SS---FR-E-E--T-E-X-T--C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-H-OL-O-G-IC-------- ------------------------------------------------------- GENERAL COWENT os -ALi tliS6Ei/i)RdA@S'N6ltl;%@ - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-ANIW-ATI-O"- - - - - - - - - - - - - - - 1U3lRtAI"llll(31R, COAGULATING OLD ICOI. LF. TESTIS (TEOI. LIVER (LI). LUNG (LU). PROSTATE IPP). SEMINAL VISICL ---------E-T-----u------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. MADISON, WISCONSIN U.S.A. INC. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CB?749 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED TERMINAL SACR DATE OF DEAYN! 03/22/91 STUDY DAY OF DEATH: 03 STUDY WEEK OF DEATH: 14 TERMINAL 600Y WEIGHT: DATE AND TIME OF NECROPSY: 03/22/91 8:12 PROSECTOR: KEVIN BILLINGS RECORDER: JOE N. KIZ POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL 13EVAN ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT OR43AN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- (GRAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- ST ... BRAINR ILBIRJ 2.1303 .4298 % 1.0000 WEI LIVE 16.2508 3.2790 % 1.6284 WEI LUNG (LU) l.solo .3224 % .?Sol WEI LF ES S TEO 1.6322 .3293 % .7662 WEI ItT: TESTIIS TEI 1.5716 .3171 % .7377 WEI ACCESS/SEX 0I0 ISO) 2.1174 S$26 % 1.3ss4 WEI ---------------------------------------------------------------------------------------------------------------- . a a a P A T H 0 L 0 0 Y 0 13S E A V A T 1 0 N S a A & ORGAN NAME ---------------------- KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC ----------------------------------------------------------------------------------- GENERAL COMMENT GI(OICSI:-ALL ttiS6[i/61tdAiS'N6Rt4AL . . ... . . LIVER ILI) . . . . . . . . . I . I . . . . . . . . . . . . . . MICRO: -MINIMAL. MULTI-FOCAL -tiFiLiltATiS'.M6N6N6CL'EAft'CiL@ PROSTATE (PRI .. ... .. . .. . .. . .. ... . Al@R6:'-@LiGAT' iO@A@ *.I'NF'LAMMATION. CHRONIC ACTIVE : ----- -- -- --- --- --- -- -- ---- --- ---- ---THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATTON: --------B-R-A-IN---(-OR-)-.--C-O-A-G-UL-A-T-I-N-G--G-L-D---IC-O-I-.--L-F-.--T-E-ST-I-S---(-TE-O-I-.--L-U-N-G--I-L-U-)-. --S-EM-I-N-A-L--V-E-S-I-CL-E---I-S-V-I.---UR-E--T--H--R-A---J-U-RP--------- t.n to INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: CG?750 DATE OF DEATH: 02/13 91 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED SECOND INTER STUDY DAY OF DEATH: 56 STUDY WEEK OF DEATH: I TERMINAL 600Y WEIO"T: DATE AND TIME OF NEC40psy: 02/13/91 7:15 PROSECTOR: OUT" SCHMITZ RECORDER: KEVIN BIL POST-FIX WEIGHER: NOY AVAILABLE PATHOLOGIST: ON. TOM PALMER WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIOHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMS) TO BODY WEIGHT WEIGHT RATIO s ---------------------- -------- -------- -------- BRAIN JOR) LIVER (Lll LUNO ILU) LF. TESTIS (TEO 1.9796 ls.s428 1.3520 1.4122 .4419 % 3.4694 % .3011 % .3152 % 1.0000 WE 7.$SIS WE .6830 WE .7134 WE R7. TESTIS @TEI 13.4860 3.0058 % 6.1024 EX ACCESS/SEX 010 ISO) 2.6175 .5143 % 1.3222 WE --------------------------------------------------------------------------------------------------------------- . a & a P A T N 0 L0 0 Y 0 8 S E k V A T 1 0 N S 1 0 A ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION OROSS FREE-TEXT COMMENTS / "ISTOPATHOLOGI -------------------------------------------------------------------------------------------------- GENERAL COMMENT GIOOCSI:-ALi iltS6Ei/bAdAiS'N6*ki THE- FO-LLO-WING- T-ISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - IURAIT"HIIII(R1,,CO*IULATING OLD (CO). LF. TESTIS ITEO). LIVER ILT). LUNG (LU), PROSTATE (PRI. SEMINAL VESIC E u --------------------------------------------------------------------------------------------------------------- %0 00 INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. maolsom, WISCONSIN U.S.A. INC. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: COYISI SEX: MALE DOSE GROUP: 0 SACRIFICE STATUS: SCHEDULED FIRST INTERI@ DATE OF DEATH: 01/25191 STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 TERMINAL IODY WEIGHT: DATE AND TIME OF NECROPSY: 01 2SI91 1:15 PROSECTON: JULIE A. LARSEN RECORDER: JILL BEVAN POS7-FIX WEIGHER; NOT AVAILAII(E PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY D ---------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 OROAN NAME (GRAMSP -------- to BODY WEIGHT (%) -------- WEIGHT RATIO -------- ST --- ---------------------- BRAIN JORI 2.0547 .5309 % 1.0000 WEI L VER Ll 14.2522 3.6127 % 6.9364 WEI LUNO (LU) 1 5778 4077 % .7679 WEI LF. TESTIS (TEO) 1.4513 .3750 % .7063 WEI RT. TESTIS ITEI 1.4922 .2156 % .7262 WEI ACCESS/SEX ORG isoi 2.4998 .0459 % 1.2160 WEI --------------------------------------------------------------------------------------------------------------- 0 a ok P A I " 0 L 0 0 Y 0 B S E P V A T 1 0 N S * * ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-IEXT COMMENTS / * HIS70PAT140LOGI( ---------------------- ----------------------------------------------------------------------------7 ----- GENERAL COMMENT IGCI, . . ... . os -ALi iliS6E@/6R6A@S NORMAL THE- FO-LLOW-ING - -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPIC EXAMINATION: BRAIN BR) COAGULATING OLD (COI. LF, TESTIS (TEO). LIVER ILIJ. LUNO ILU). PROSTATE IPR). SEMINAL VESICL URETHRI (U*l -------- ------------------------------------------------------------------------------------------------- at C3, CDI INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C077S2 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: S7 STUDY WEEK OF DEATH: 9 TERMINAL 60DV WEIGHT: DATE AND TIME OF NECROPSY: 02(14/91 7:18 PROSECTOR: DENNIS NOFF14AN RECORDER: JILL 13EVA POST-FIX WEIGHER: FOOT AVAILAB E PATHOLOGIST: ON. TOM PALMER WEIGHER: VAL QUINN -------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELA71VE ORGAN 70 BRAIN 0 OROAN NAME (ONAMS) TO BODY WEIO"T WEIGHT RATIO s ---------------------- -------- -------- -------- "RAIN (941 2.1100 .4449 % 1.0000 WE LIVER ILI) 18.8719 3.$S&4 % 8.6561 WE LUNG LLUI 1.5144 .3233 % .7268 WE LF TE TI OITIEOISOI 1.7674 .3607 % .8107 WE PT.' TESTIS TEI 1.73se .3542 % .7901 WE ACCESS/SEX 0 2.6$11 s431 % 1.2207 WE --------------------------------------------------------------------------------------------------------------- a a 0 P A 7 " 0 L & 0 V 0 8 S E R V A 7 10 N S h A & ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION OROSS FREE-TEXT COMMENTS / HISTOPATHOLOOT -------------------------------------------------------------------------------------------------------- GENERAL COMMENT 04GOCSI:-ALC iliS6Ei/61tdAkS'N6ftiiAi . . . . - -THE- FO-LLO-WINO- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - - - - - - - BRAI COAGULATING OLD (CO). Lf. TESTIS ITEO), LIVER (LI). LUNG (LU). PROSTATE IPRI. SEMINAL VISIC URETH"RI,I,U*l ------------------------------------------------------------------------------------------------------------ INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C6?753 SEX: 14ALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED SECOND INTERI DATE OF DEATH: 02/14/91 STUDY DAY OF DEATH: 57 STUDY WEEK OF DEATH: 9 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02/14/91 7:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL BEVAN P-O-S-T--F-I-X--W-E-IG-H-E-R:---N-O-T --AV-A-I-LA-B-L-E ----------------P--A--T-H-O--L--OG-I-S-T:----DR-.--T-O-M--P-AL-M-ER-----------------WE-I-G-HE-R-:---VA-L--Q-U-IN-N----------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMSI -------- TO BODY WEIGHT 4%1 -------- WEI-GH-T---R-AT-I-O ST ... ---------------------- BRAINn JLOIRJ 1.9936 .4191 % 1.0000 WEI LIVE I LUNG (LU) 21.3720 1.6053 4.492.7 % .3375 % 10.7203 WEI .8052 WEI LF ESSITIIS TE? 1.6930 .3SSO % .8492 WEI ItT@ TTE TE 1.7542 .3688 % .8799 WEI ------A-C-CE-S-S-/S-E-X---0-1-0---I-SO-)--------------3-.-18-3-1-------------------.-60-9-1--% --------------I-.-S-96-7-------------WE-I--------------- a a e P A T " 0 L 0'(1V 0 0 S E R V A T 1 0 N S * 6 a O-R-GA-N---NA-M-E--------------KEYW-O-RD-S-.---SE-V-E-RI-T-Y-. --D-I-S-TR-I-B-UT-I-O-N----------G-R--O-S-S--F-R-E-E--T-E-X-T ---C-OM-M-E-N-TS----/---"I-S-T-OP-A-T-HO-L-O-GI-C------ GENERAL COM14ENT JOC GROSI: -ALL iliS6Ei/6R6AiS*NbRkAi . . . . . . .. .. . .. .. .. .. .. . - -THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-E14A-RKA-BLE - AT- MI-CROS-COPIC EXAMINATION.BURRAIN"RIBRI. COAGULATING GLD (COI, LF. TESTIS (TEO). LIVER ILI), LUNG ILU). PROSTATE (PR). SEMINAL VESICL --------------E-T-------(-U-R-I--------------------------------------------------------------------------------------- as INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67754 DATE Of DEATH: 03/25191 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED TERMINAL SACR STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: PROSECTOR: JACK SERFORT RECORDER: JOE H. KIZ POSI-FIX WEIGHER: H07 AVAIL239(2ES/91 7:56 PATHOLOGIST: DR. TOM PALMER WEIG14EN: STEVE VAN A ---------------------------------------------------------------------------------------------------------------- ABSOLUTEORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME IGRAMS) -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- ST --- ---------------------- RIAIN (841 2 1790 18.1130 .3990 % 3.321.4 % 1.0000 WEI 8.3416 WEI L VER ILI LUNG (LU) 1.7299 .3167 % .7936 WE L I7 TEOI 1.7984 1.8016 .3222 % .3311 % .8250 WE .1297 WE VYF@ TE'STISS TEI ACCESS/SEX 010 ISO) 4.4033 .1060 % 2.0200 WE --------------------------------------------------------------------------------------------------------------- A a & P A T H 0 L 0-0 V 0 8 S E N V A T 1 0 N S a h a OROAN NAME K-E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---D-IS-T-R-I1-3-U-TI-O-N---------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---H-IS-T-O-PA-T-H-OL-O-G-I------- ---------------------- . .. .. . GENERAL COMMENT OIGOCSI:-iLi iliS6E@/6N6AiS'NdRAAC LIVER (LI) . .. ... . .. .. . I .. . .. .. .. . MICRO: -MINIMAL. FOCAL - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN fgftl. COAGULATING OLD (CO). LF. TESTIS (TEO). LUNG ILU), PROSTATE IPP). SEMINAL VESICLE (SVI. URE --------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZTETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C@7755 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED TERMINAL SAC DATE OF DEATH: 03/22 91 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: DATE AND 71HE OF NEC40PSY: 03/22/91 9:27 PROSECTOR: KEVIN BILLINGS RECORDER: JOE W. KI POS7-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: JILL BEVAN --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- JORAMSI -------- TO BODY WEIGHT -------- WEIGHT RATIO -------- s BIRAIN JONI 2.1551 .4sso % 1.0000 WE L VER (Lll 13.96SO 2.9481 % 6.4800 WE LUNG ILU) 1.7195 .3030 % .7972 WE LF TESTIS TEOI 1.7221 .2637 % .7994 WE RT@ TESTIS TEI 1.73$S .3670 % .2067 WE ------A-C-CE-S-S-/S-E-X---0-1-0--(-S-O-)--------------2-.2-2-3-1-------------------.-48-2-0--% --------------1-.-05-9-4------------W-E--------------- a ok & P A T H 0 L 0.0 Y 0 0 S E R V A T 1 0 N S A A A ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COWENTS / HISTOPATHOLOGI ---------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER ILI) ol'co.os.-tA:.L@. i.li.S6.Ei/.6R.dAi.S'.N6.RAA.@ . . . . . . . . . . MICRO: -MINIMAL, FOCAL -liFiLiNATiS:m6N6N6c@EAR'CiLi THE- FO-LLOW-ING- T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - - - - - - - BRAIN (OR). COAGULATING GLO (CGI, LF. TESTIS ITEOP. LUNG (LU). PROSTATE (PR). SEMINAL VESICLE ISV), UNE --------------------------------------------------------------------------------------------------------------- Ob INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67756 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED TERMINAL SACRI DATE Of DEATHI 03/26/91 STUDY DAY Of DEATH: 97 STUDY WEEK Of DEATH: L4 TERMINAL 6ODY WEIGHT: 4 DATE AND TIME OF NECROPSY: 03/26/91 7;10 PROSECTOR: STEVIE VAN ADESTINE RECORDER: JILL BEVAN P-O-S-T--F-I-X --W-E-IG-H-E-R:---H-OT--A-V-AI-L-A-BL-E-----------------P-AT-H-O-LO-G-I-ST-:---O-N-. --T-OM--P-A-LM-E-R----------------W-E-I-GH-E-R-:--J-O-E--M.--I-(-I-ZZ-I--------------- ABSOLUTE ORGAN WEIGHT ORGAN NAME ---------------------- -(-G-R-AM-S-I-- ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN WEIGHT RATIO -------- 0R ST ---- BRAIN JOR) 2.0271 .4137 % 1.0000 WEIG LIVER LI) 16.321S 3.3323 % i.ossi WEIG LUNOTCLLUI 1.5033 .3001 % .7416 WEIG Lf, 71 1.54S3 .3tS4 % .7623 WEIG NY. TESTIS IEI 1.5535 .3170 % .7604 wlto ACCIESS/SEX ollaeolsol 3.0065 .6136 1 l@4832 WEIG ---------------------------------------------------------------------------------------------------------------- A.a * P A T H 0 L 0.0 Y 0 0 S E I V A T 1 0 N S INa a O-R-G-A-N--N-A-ME-------------K--E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-TS----/---HI-S-T-OP-A-T-H-OL-O-G-IC-------- GENERAL LIVER COMMENT GI'OGCSI:-ALi iliSUE@/bltdAkS'N6RkA@ (LI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . MICRO! -MINIMAL, FOCAL -INFILTRATES,MONONUCLEAR CELL THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - - - - BRAIN (OR), COAGULATING GLD (CGI, LF. TESTIS ITEO). LUNG ILU). PROSTATE (PA). SEMINAL VESICLE (SVI, URETH ----------------------------------------------------------------------------------------------------------------- at INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. ----------------------------------------------------------------------------------------------------------------- ANIMAL HUP48ER: C$7757 SEX: MALE STUDY DAY Of DEATH: DOSE 37 GROUP: a STUDY WEEK SACRIFICE STATUS: Of DEATH: 6 SCHEDULED FIRST INTERIM TERMINAL 6ODY WEIGHT: 4 DATE Of DEATH: 01/2S/91 DATE AMD TIME OF NECROPSY: 01/25/91 9;23 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL BEVAN POST-FIX WEIGHER: NOT AVAILABLE PA114OLOGIS7: DR. 7(*4 PAL14EN WEIGHER: PATHOLOGY DE ----------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0p OROAN NAME JORANSI -------- TO BODY WEIGHT -------- WEI-GH-T---R-AT-I-O ST ---- ---------------------- BRAINIt JON11 LIV L 2.1107 16.2700 S410 % 4.0364 % 1.0000 WEIG 7.4613 WEIG LUNG (LUI 1.5371 1.0187 .3$IS % .4016 % .7052 WEIG .1423 WEIG LF. S, is E OT. 1T1ESTIS ITTE 1 1.6301 .4046 % .7418 WEIG ACCESS/SEX 0 0 olsol 2.910$ .7221 % 1.3346 WEIG ------------------------------------------------------------------------------------------------------- a a a P A 7 N 0 L 0--GV 0 9 S E 0 V A T 1 0 N S & a 9. ORGAN NAME K-E-Y-W-O-R-D-S-,-S-E-V-E-R-I-TY-.--D-I-S-TR-I-B-U-T-I-O-N--------G--R--O-S-S-- F-R-E-E--T-E-XT---C-O-MM-E-N-TS----/--"-I-S-TO-P-A-TH-O-L-OG-I-C-------- ---------------------- OENERAL COMMENT 04GOCSI: -ALL il@S6E/61tdAkS'N6RAAi . . . . . - -THE- FO-LLO-WING- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MICRO-SCOP-IC - EX-AMI-NA71-ON:- - - - - - - - - - - - - - - BRA ),,COAGULATING OLD (CGI. LF. TESTIS (TEO). LIVER (LI), LUNG (LU). PROSTATE IPRI. SEMINAL VESICLE -------U-R-E-TIH-NR-IB-R-(-U ------------------------------------------------------------------------------------------------- cpb C) Ch INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7751 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE OF DEATH: 02/14/21 STUDY DAY OF DEATH; S? STUDY WEEK OF DEATH: 9 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 02/14/91 9;01 PROSECTOR: NUT" SCHMITZ RECORDER: JILL SEVA POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: VAL QUINN --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- --(G-R-A-MS-I-- To BODY WEIGHT 1%) -------- WEIGHT RATIO -------- s OIAIXR 1L8IJ 2.1971 14.6s2y 4932 % 3.2843 % 1.0000 WE 6.0073 WE L VE LUNG ILU) l.s9l4 .3SY1 % .7241 WE LF. TESTIS ITEO) I.S$23 .3SSI % .7200 WE Ky. TESTIS TEI 1.64AS .3091 % .7413 WE -------AC-C-ES-S-/-SE-X---O-l-e---I-SO-)--------------2-.9-2-9-6-------------------.-65-7-5--% --------------1-.3-3-3-0------------W-E -------------- a A a P A T " 0 L ib0 Y 0 0 S E R V A T 1 0 N S - A O-R-GA-N---MA-1-4E-------------K-E-Y-WO-R-D-S,---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------O--R--O-S-S---FR-E-E--T-E-X-T--C-O-MM-E-N-T-S ---/---"I-S-T-O-PA-T-H-OL-O-O-I------- GENERAL LIVER COMMENT 0400CSI:-ALi itiS6Ei/61tdAiS't46Ri4Ai (LI) . i4l6Ri):'-i4thIkA@,'MuLil-FbC.iL. . . .. .. . * :IiFiLiRATiS'146N6N&ciEAR'CiLi . . . . . . . - -THE- FO-LLO-WTHO- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMIN-ATI-ON: - - - - - - - - - - - - - BRAIN 1891, COAGULATING GLO ICOI, LF. TESTIS (TEO), LUNG (LU). PROSTATE IPRI, SEMINAL VESICLE ISVI. URE --------------------------------------------------------------------------------------------------------- Ch INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- AllIP4AL NUMBER: C07IS9 SEX: 14ALE DOSE GROUP: 0 SACRIFICE STATUS: SCHEDULED. FIRST INTERI DATE OF DEATH: 01/24 91 STUDY DAY OF DEATH: 30 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: DATE AND TIME OF NEC40PSY: 01/24/91 7:06 PROSECTON: KEVIN BILLINGS RECORDER: VAL QUINN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TO#4 PALMER WEIGHER: DEBBIE PIN --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRANSI TO BODY WEIGHT 1%) WEIGHT RATIO s ---------------------- -------- -------- -------- AIN OLRIVER IBLNil LUNG ILU) 2.0779 IS.2773 1.4004 Sias % 3.79?S % .3411 % 1.0000 WE 7.3523 WE .6739 WE LF. TESTIS 11101 1.5086 .3049 % .7845 WE OT. TESTIS 1.4005 .3705 % .7173 WE ACCESS/SEX ONG )Sol 1.97?S .40is % .9sly WE --------------------------------------------------------------------------------------------------------------- . a * a PA T " 0 L 0 0 Y 0 8 S E N V A T 1 0 N S a * 0 ORGAN NAME KEYWORDS,SEVERITY.DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGI -------------------------------------------------------------------------------------------------------- GENERAL COMMENT GAOSSCI: -ALi iliS6Ei/6lt6AiS*N61tki THE- FO-LLOW-ING- -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COPIC EXAMINATION: BRAI COAGULATING OLD (CO). LF. TESTIS (TEO). LIVER ILIJ, LUNG (LU). PROSTATE (PRI, SEMINAL VESIC Upt[T""Itl",(tAuI, ----------------------------------------------------------------------------------------------------------- as 4@ INDIVIDUAL APPENDIX D ANIMAL PATHOLOOY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S.A. --------------------------------------------------------------------------------------------------------------- AN104AL NUMBER: C67760 SEX: MALE DATE Of DEATH: 03/22 91 STUDY DAY OF DATE AND TIME OF NEC(OPSY: 03/22/91 8:49 POST-FIX WEIO"Ell: NOT AVAILABLE DOSE GROUP: 6 DEATH: 93 SACRIFICE STUDY WEEK OF DEATH: PROSECTON: RUTH SCHMITZ PATHOLOGIST: DR. TOM PALMER STATUS: 14 SCHEDULED TERMINAL SAC TERMINAL 6ODY WEIGHT- RECORDER: JOE N.'Ki WEIGHER: JILL BEVAN ORGAN NAME ABSOLUTE OROAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ---------------------- (ORANSI -------- TO BODY WEIOHT (Zi -------- WEIGHT RATIO 0 s BRAIN LIVER ILBItl 2.1114 15-0452 .42S$ 1 3.IS3$ % -------- 1.0000 WE 7.4099 LUNG LU) LF. TELTIS ITEO1 1.0650 1.5040 .33SS % WE .7880 WE AT. TESTIS ITE 1.5050 .3153 % .7407 WE ACCESS/SEX Ono ISO) 2.?SS7 .31SS % .7412 WE -------------------------------------------------------------SS-?-S--% --------------1-.-30-9-9------------W-E--------------- ORGAN NAME a a * PA T N 0 L 0 0 T 0 0 S E 4 V A T 1 0 N S a * a ---------------------K--E-Y-W-OR-D-S-, --S-E-V-ER-I-T-Y,---D-IS-T-R-IB-U-T-IO-N----------O-R-O-SS FREE-TEXT COMMENTS / HISTOPATHOLOGI GENERAL COMMENT c ------------------------------------------- Gloost: -AL@ iliS6Ei/6R6AiS*N6Ri4A@ . . . . . ... . THE- FO-LLOW-INOI -TISS-UES- WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - E X-AMI-NATION:- - - - - - - - - - - - - - - BURREATIHNR IOR)U,,COAOULATING OLD ICGI, LF. TESTIS (TEOI. LIVER (LI). LUNO (LU). PROSTATE IPRI. SEMINAL VESICL -------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON MADISON, LABORATORIES AMERICA. WISCONSIN U,S.A. INC. ---------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67761 SEX; MALE DOSE GROUP: 6 DATE OF DEATH: 01/2S/91 STUDY DAY OF DEATH: 37 SACRIFICE STATUS: SCHEDULED. FIRST INTERIM DATE AND TIME OF NECROPSY: 01/2S/91 7:4S STUDY WEEK OF DEATH: 6 PROSECTON: JULIE A. LARSEN TERMINAL BODY WEIGHT: 3 POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER RECORDER: JACK SERFOR ---------------------------------------------------------------------------------------W-E-IG-H-E-R:----P-AT-H-O-LO-G-Y---D-E------- ORGAN NAME ---------------------- ABSOLUTE ORGAN WEIGHT (GRAMS) -------- ORGAN WEIGHT RELATIVE TO BODY WEIGHT -------- ORGAN TO BRAIN WEIGHT RATIO -------- 0A ST BRAIN LIVER ILBItl 2.0687 IS.4sel s279 % 3.9439 % 1.0000 7.4714 ---- WEIG WEIG LUNG (LU) LF. TESTIS ITT E 1.4140 l.s977 .3008 % .6835 WEIG NT. TESTIS E0ll I-SO25 .4077 % .4064 % .7723 WEIG -------A-CC-E-S-S/-S-EX----0--0----S-o-l ---------------2-.-69-6-1---------------------.-6-10-0--%----------------1.-.3-706-39-38-------------WW-EE-II-GG ------- ORGAN NAME a ok* P A T H 0 L CY'G Y 0 8 S E A V A T 1 0 N S a h 0 ---------------------- K-E-Y-W-OR-D-S-.---S-E-VE-R-I-TY-,---D-I-S-TR-I-B-UT-I-O-N-----------GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC GENERAL COMMENT ------------------------------------I------- GA'OCSI: -iLi fliSOEi/6ft6AiS'N6RkA@ . . . . . . . . .. - -THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COPI-C - EX-AMIN-ATIO-N: - - - - - - - - - - - - - - - BRAI.N.187),,COAGULATING OLD ICO). LF. TESTIS (TEO). LIVER (LI), LUNG (LU). PROSTATE (PRI, SEMINAL VESICLE UNE u ------------------------------------------------------------------------------------------------------------ c:o INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A. ---------------------------------------------------------------------------------------------------------------- ANIMALNUMBER: C$7762 DATE OF DEATH: 01/23/91 SEX: MALE DOSE GROUP: a SACRIFICE STATUS: SCHEDULED. FIRST INTERIM STUDY DAY OF DEATH: 3S DATE AND TIME OF NECROPSY: 01(23/91 1:21 STUDY WEEK OF DEATH: S TERMINAL BODY WEIGHT: POST-FIX WEIGHER: NOT AVAILAS E PROSECTOR I NANCY DI EDRICH PAT"OLOG S:T: DR. T014 PALMER RECORDER: JILL BEVAN --------------------------------------------------------------------------------WE-I-G-HE-R-:---GA-I-L--M-.--NO-V--------------- ORGAN NAME ABSOLUTE ORGAN WEIBHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ---------------------- JORAMSI -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO 0 ST BRAIN 2.0710 -------- --- LIVER LUNG ls.ises .5327 % 4.3103 % 1.0000 WEI 8.0920 LF. TEILTUII ITEO11 l.so2o 1.6769 .4097 % WEI .7691 WEI NT. TESTIS ITE 1.6002 .4313 % .8097 WEI ACCESS/SEX ORO (SO) 2.6627 .4116 % .7727 WEI - - - - - - --- ----- --.-61--49--%-- - - - - - - 1.285? WEI ORGAN NAME P A T 0 L 0.0 Y KEYWORDS, SEVERITY, DISTRIBUTION 0 8 S E R V A T 1 0 N-S---;-;-;---------------- ----------------------------------------------------------G-R-O-SS FREE-TEXT COMMENTS / "ISTOPAT"OLOOIC GENERAL COMMENT -------------------------------------------- LIVER (LI) 04'OCSI: -ALL. -TI-SS.UE.S/0-RdAiS*N6RkAi . . . . . . . .. . ... . -INFILiPtATES.W.MONUC@EAR'CiLi THE FOLLOWING IISSUES WEItIE-UPO-RE1-4AIt-KABL-EAT- MI-CROS-COPI-C - EX-AMIN-ATIO-N - --- - - - - - - - - - - - - BRAIN (BRI. COAGULATING GLD (CO). LF. TESTIS FTEO). LUNG (LUI. PROSTATE (PRI. SEMINAL VESICLE (SV). URETH ------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES . A14ERICA, INC. K401SON. WISCONSIN U S.A. --------------------------------------------------------------------------------------------------------------- ANlt4ALNUMBER:C677o3 SEX: MALE DOSE GROUP: 6 DATE OF DEATH: 02413@ol STUDY DAY OF DEATH: 56 STUDY WEEK OFSACDREIAFTIHC:E I STATUS: SCHEDULED SECOND INTER DATE AND TIME OF CC OPSY: 02/13/91 POST-FIX WEIGHER: NOT AVAILABLE 1:38 PROSECTON: RUTH SCPL41TZ TERMINAL 6ODY WEIGHT: RECORDER: KEVIN OIL -----------------------------------------P-A-TH-O-L-OG-I-S-T:----DR-.--T-O-M--PA-L-1-4E-R----------------WE-I-G-HE-R-:---JI-L-L--B-EV-A-N--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ---------------------- IGRAt4SI -------- TO BODY WEIGHT 1%) -------- WEIGHT RATIO 0 s BRAIN Joni 2.0315 .4248X -------- LIVER ILI) LUNG LU) IF TEITIS TEO 18.16is 1.6338 1.7633 3.9303 % .3404 % 1.0000 WE 9.2S20 WE lots WE ItT: TESTIS TEI 1.7638 .3074 % .8650 WE ACCESS/SEX 01 a )Sol 2.4933 .367S % Iasi WE -------------------------------------------------------------------.5-1-9-S --x --------------1.-2-2-31-------------W-E------- OROAN NAME - ------------KEYWORDS. fta It P A T " 0 L 0 a Y SEVERITY. DISTRIBUTION - 0 0 S E R V A T 1 0 N S ft ok GROSS FREE-TEXT COMMENTS MANDIB/CERV IN - ------------------------------- --------------------------HI-S-T-OP-A-TH-O-L-OG-I--- AR""OIS: -iAiGi . . . . . . . . . . . . . . . . . .RI.G.HT.!U.P.TO. l.e.X.9 .X .5 .MM. (.F.O.R.CO.M.PA.R.IS.ON MICRO: -MODERATE. MULTI-FOCAL (CAP 61 -HYPERPLASIA. LYMP"ORETICULAR CELL THE- FO-LLO-WINO- T-ISS-UES- WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMIN-ATI-Olf:- - - - - - - - - - - - - - - BURREATIHINVIBRIUIAL COAGULATINO OLD (Col. LF. TESTIS (TEOI, LIVER (Lll, LUNG ILUI. PROSTATE (PR). SEMINAL VESICL ---- ---------------------------------; --------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- AN114AL NUMBER: C$7704 DATE OF DEATH: 01125191 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED FIRST INTEitl STUDY DAY OF DEATH: 37 DATE AND TIME OF NEC70PSY: 01/2S/91 9!17 STUDY WEEK OF DEATH: 6 TERMINAL 6ODY WEIGHT: POST-FIX W[IONER: NO AVAILABLE PROSECTOR: NANCY DIEDRICH RECORDER: JILL BEVA ------------------------------------------P-A-T-HO-L-O-GI-S-T-: --O-R-,--T-O-N--PA-L-M-ER-----------------WE-I-GH-E-R-: ---PA-T-HO-L-O-GY----------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ---------------------- -------- TO BODY WEIGHT 1%) -------- WEI13HT RATIO -------- s BRAIN 8 LIVER ILOIJ 1.0914 Solo % 1.0000 WE LUNG ILU) If.SS57 1.7763 4.2234 % 1.3136 WE LF TESTIS RT: TESTIS TEO11 TE 1.6393 .4531 % .4182 % .8920 WE .8232 WE ACCESS/SEX 010 ISO) 1.0390 2.4532 .4111 % .8230 WE ------------------------------------------------------------.-6-2S-$--%--------------1-.-23-1-9-------------WE--------------- ORGAN NAME 9b* * P A T H 0 L 0 0 Y 0 8 S E R V A T 1 0 N S a 0 a KEYWORDS. SEVERITY. DISTRIBUTION G-E-NE-R-A-L ---CO-M-ME-N-T-----c-----------------------------------------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-I ------- URETHRA JUR) 04'osl: -ALL it@SOE@/6fldAOS'N6ltkAi ... .. .. . .. .. .. . .. .. .. . MICRO: -SECTION EXAMINED; TISSUE NOT PRESENT .. .. .. . . .. .. .. THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KABL-E - AT- MI-CROS-COP-IC - EX-AMINATION.BRAIN (OR). COAGULATING GLD (CGI. LF. TESTIS (TEOP, LIVER (LI). LUNG ILUI, PROSTATE (PRI. SEMINAL VESIC ------------------------------------------------------------------------------------------------------------- Ch w INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$??eS SEX: MALE DOSE GROUP: a DATE Of DEATH: 02/13 91 STUDY DAY OF DEATH: So DATE AND TIME OF NIC40PSY: 02(13/91 8:45 SACITIFICE STATUS: SCNEDULED SECOND INTER STUDY WEEK OF DEATH: I TERMINAL 60[)Y WEIGHT: POST-FIX WEIGHER: NOT AVAILAO E PITOSECTON: DENNIS HOFFMAN RECORDER: KEVIN OIL ------------------------------------------P-AT-H-O-LO-O-I-ST-:---D-R-.--T-OM--P-A-LM-E-R----------------W-E-IG-H-E-R-: --J-I-L-L --BE-V-A-N--------------- ORGAN NAME ABSOLUTE ORGAN WEIGHT JORAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT ORGAN TO BRAIN 0 ---------------------- -------- -------- WEIGHT RATIO s BRAIN LIVER ILBRII 2.ISO6 .4916 % -------1.0000 LUNG LU) 16.4708 3.7493 % WE 7,6268 LF. TEITIS 01TEOISO, 1.74SY I.S511 .3974 % .8083 WE WE RT TESTIS TEI 1.548S .3532 % .7116 WE AC@ESS/SEX 0 2.9217 .3525 % .7170 WE ------------------------------------------------------------O-e-s-i--x--------------1-.3-S-2-9------------W-E --------------- ORGAN NAME @ a a 6 P A I t0oU L Q 0 Y 0 8 S E N V A T 1 0 N S * A 4 ---------------------K--E-Y-WO-R-D-S,---S-E-VE-R-I-TY-,---DI-S-T-RH----T-O-N---------G-RO-SS- FREE-TEXT COMMENTS / HISTOPATHOLOGI GENERAL COMMENT c ------------------------------------- GA'osl: -AL@ iliS6Ei/6R6AiS'N6ltAAC .. .. . .. .. . .. . -- --- -- -- -- ---- --- -- --- -- --- --- --- - THE FOLLOWING TISSUES WERE UMREMARKA13LE AT MICROSCOPIC EXAMINATION: OUR,ETTAHIRNA (O(RU)R.I COAGULATING OLD (CO). LF. TESTIS (TEOI, LIVER (LI). LUNG (LU). PROSTATE (PRI. SEMINAL VESIC ---- ----------------------------------------------------------------------------------------------------- ot INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON MADISON. LABORATORIES AMERICA. WISCONSIN U.S.A. INC. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7766 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SC14EDULED SECOND INYER DATE OF DEATH: 02/12 $1 STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL 6ODY WEIGHT: DATE AND TIME OF NEC40PSY: 02/12/91 7:20 PROSEC70R: KEVIN BILLINGS RECORDElt: DENNIS N POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY -------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIO"T ORGAN WEIGHT RELATIVE ORGAN TO BRAIN ORGAN NAME ---------------------- (GRAMSI -------- 70 BODY WEIGHT -------- WEIOHT RATIO s -------- BRAIN (841 LIVER ILI) LUNG (LU) i.osol 12.4009 1.4S26 .4994 % 3.1603 % .3702 % l@0000 WE 6.3276 WE 7412 WE LF. TESTIS TEO 1.5927 .40SO % .8127 WE AT. TESTIS TEI 1.5625 .3912 % .7973 WE ACCESSISEX 010 lsol 2.SS64 .7279 % 1.4S75 WE -------------------------------------------------------------------------------------------------------------- kt a a P A I 10 L 0 0 Y 0 0 S E ItV A T 1 0 " s a 0 ORGAN NAME ---------------------- KEywoltoS. SEVERITY. DISTiNt BUTIBN GROSS FREE-TEXT COMMENTS / - HISTOPATHOLOGI ---------------------------------------------------------------------------------- GENERAL LIVER COMMENT (LI) GIOOCSI: -ALi iliS6[i/6RdAiS'N61ti4Ai . MICRO: -MINIMAi,*F6CAL* . . . . ... . :IhfiLi#tATiS'M6"6N6CifAft*CiLi --- -- -------- -- -- --- --- --- -- ---- -- --THE FOLLOWINGTISSUESWEREUNREMARKABLAET MICROSCOPIECXAMINATION: BRAIN (OR). COAGULATING GLD (CO). LF. TESTIS (TEO). LUNG ILU). PROSTATE (PR). SEMINAL VESICLE ISVI, URE ------------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX D ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. -------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C$7767 DATE OF DEATH: 03/25/91 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED TERMINAL SAC STUDY DAY OF DEATH: 96 STUDY WEEK OF DEATH: 14 TERMINAL 6ODY WEIGHT: DATE AND TIME OF NECROPSY: 03/25/91 7:45 PROSECTOR: JULIE A. LARSEN RECORDER: JOE N, KI POST-FIX WEIGHER: ROY AVAILABLE PAY140LOOIST- OR. TOM PALMER WEIGHER: STEVE VA14 --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMSI TO BODY WEIGHT WEIGHT RATIO s ---------------------- -------- -------- -------- BIAIRN (!*1 2.2220 .4410 % 1.0000 WE L VE 1 1 16.1837 3.2117 % 7.2134 WE LUNG ILU) l.s364 .3649 % .6914 WE LF TEST S YE 1.6959 .3360 % .7632 WE RT: TESTIS TEI 1.7503 .3474 % .1177 WE ACCESS/SEX JO olso) 3.0260 .600S % 1.3610 WE --------------------------------------------------------------------------------------------------------------- 6 A * P A T H 0 L'O 0 Y 0 8 S E R V A I 1 0 N S A 1%0 ORGAN NAME KEYWORDS, SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPAT"OLOGI -------------------------------------------------------------------------------------------------------- GENERAL COMMENT LIVER (LI) GI(DICSI-:iLi iliS6Ei/61ldAiS*N6Rki MI@R6:*-iLiG'HT: iO@Ai -INFILTRATES,MONONUCLEAR CELL THE- FO-LLOW-ING- -TISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COPI-C - EX-AHIN-A71-ON: - - - - - - - - BRAIN ISR). COAGULATING OLD ICOI, LF. TESTIS (TEOP. LUNG ILUI. PROSTATE IPRI, SEMINAL VESICLE (SVI. URE ----------------------------------------------------------------------------------------------------------- APPENDIX 0 TNDTVTDUAL ANIMAL PATHOLOGY DATA HAZLETON LABORATDRIES AMERICA, INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67761 SEX: 14ALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED SECOND INTER DATE OF DEATH: 02/12/91 STUDY DAY OF DEATH: SS STUDY WEEK OF DEATH: I TERMINAL ADDY WEIG147: DATE AND TIME OF NECHOPSY: 02/12/91 1:28 PROSECTON: NANCY DIEDRICH RECORDER: DENNIS N( POST-FIX WEIGHER: NOT AVAILABLE PAY14OLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY -------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELA71VE ORGAN TO BRAIN OROAN NAt4E JORAMS) TO BODY WEIOHT 1%) WEIGHT RATIO s ---------------------- -------- -------- -------- RA IN A 0LIVER IBLII 2.0700 17.7753 .4734 % 4.0648 % 1.0000 WE 1.5871 WE LUNG 1.7744 .4058 % .1512 WE LF. TEILTUIL TEOI 1.1269 .4178 % .8826 WE RT. TESTIS ITEII 1.7633 .4032 % .8518 WE -------AC-CE-SS-/-SE-X ----O-RO----IS-OP---------------2.-7-2S-2 --------------------.-0-23-2--%---------------1-.--3-1--&--S--------------WE- * go$I P A T " 0 L-0 0 V 0 8 S E R V A 7 1 0 H S * * * O-R-G-AN---NA-M-E--------------KE-Y-W-OR-D-S-. --S-E-V-ER-I-T-Y.---D-IS-T-R-IB-U-T-IO-N----------G-R-OS-S--F-R-EE---T-EX-T---CO-M-M-EN-T-S----/ --M-II S-T-O-PA-T-HO-L:--O-O------ GENERAL COMMENT . .. . .. .. . .. .. GAGOCSI:-ALi iliS6Ei/6R6AiS*N6Ri4Ai LIVER ILI) ... .. . .. ... I . ... .. .. . .. . . MICRO: -MINIMAL. FOCAL -liFiLiRi7iS:046146N6-Cif-iR'-CiL-i - - - - -THE- FO-LLO-WINO- -TISS-UES- WE-RE -UNR-EMA-RKAB-LE - AT- MI-CRO-SCOP-IC - EX-AMI-NATI-ON:- - - - BRAIN (BRI. COAOULATING OLD (CGI. LF. TESTIS (TEO). LUNG (LU). PROSTATE (PRI. SEMINAL VESICLE ISVI. URE ----------------------------------------------------------------------------------------------------------- INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA MAZLETDN LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67769 DATE Of DEATH: Ot/2S/gi SIX: MALE DOSE GROUP! 0 SACRIFICE STATUS: SCHEDULED, FIRST INTERI STUDY DAY Of DEATH: 37 STUDY WEEK OF DEATH: 6 TERMINAL 13ODY WEIGHT: DATE AND TIME OF NECROPSY: 01/25/91 7:19 PROSECTON: NANCY DIEDRICH RECORDER: JACK SERF POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. TOM PALMER WEIGHER: PATHOLOGY --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME (GRAMS) TO BODY WEIGHT WEIGHT RATIO s ---------------------- -------- -------- -------- BIAINR 1L8IJ L VE LUNG ILU) LF. TESTIS ITEOI 127..13250042 1.7430 1.6996 .5265 % 4.30$S % .4128 % .4221 % 1.0000 WE I.il2S WE .8220 WE .1als WE RT. TESTIS (TEI 1.7401 .4321 % .0200 WE ACCESS/SEX DAG (1 Sol 2.2768 SOS4 % 1.0731 WEI --------------------------------------------------------------------------------------------------------------- . 0 a a P A I N 0 L.0 0 Y 0 8 S E P V A T 1 0 N S 0 110 ORGAN NAME KEYWORDS. SEVERITY. DISTRIBUTION GROSS FREE-TEXT COMMENTS / HISTOPATHOLOGIC -------------------------------------------------------------------------------------------------------- GENERAL COMMENT ,AGCI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . OSS: -ALL TISSUES/ORGANS NORMAL THE- FO-LLOW-ING- T-ISS-UES -WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COPIC EXAMINATION-. BRAINHitIB( R),,COAGULATING OLD [CO). Lf. TESTIS (TEOI. LIVER ILIJ. LUNG ILU). PROSTATE (PRI, SEMINAL VISICL URET u ------ ------------------------------------------------------------------------------------------------------- co INDIVIDUAL APPENDIX 0 ANIMAL PATHOLOGY DATA HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A. --------------------------------------------------------------------------------------------------------------- ANIMAL NUMBER: C67710 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED. FIRST INTERI STUDY DAY OF DEATH: 37 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: DATE OF DEATH: 01/25/91 DATE AND TIME OF NECROPSY: 01(2S/91 1:53 PROSECTOR: JULIE A. LARSEN RECORDER: JILL BEVA POST-FIX WEIGHER: NOT AVAILAO E PATHOLOGIST: DR. TOM PALMER WEIG14EN: PATHOLOGY --------------------------------------------------------------------------------------------------------------- ABSOLUTE ORGAN WEIGHT ORGAN WEIGHT RELATIVE ORGAN TO BRAIN 0 ORGAN NAME ---------------------- 113RAMSI -------- TO BODY WEIGHT (%I -------- WEIGHT RATIO -------- s BRIAINR 1L0RIJ 2.0136 13-0996 ssss % 3.6137 % 1.0000 WE G.SOSG WE L VE LUNG (LU) 1.5011 .4141 % .145S WE TESTIS TEEOI 1.5705 .4332 % .7799 WE LIFNT: TESTIS 1.4455 .4098 % .7377 WE ACCESS/SEX 010 ISO) 2.6226 .7235 % 1.3024 WE --------------------------------------------------------------------------------------------------------------- 0 a A P A T H 0 L'O 0 Y 0 9 S E R V A 7 1 0 N S 0 * A O-R-GA-N---NA-M-E-------------K--E-Y-WO-R-D-S.---S-E-VE-R-I-TY-.---DI-S-T-RI-B-U-TI-O-N----------G--R--O-S-S---FR-E-E--T-E-X-T--C-O-M-ME-N-T-S---/---HI-S-T-OP-A-T-H-OL-O-G-I ------- GENERAL COMMENT LIVER ILI) 04(OICSI-:ALi tliS6Ei/6ft6AkS*Nbfti4A@ . . . . . . i4l6R6:'-iLiGkT' iO6A@ :IiFiLiltiTiS'm6N6N6CiEilt'CiLi - - -- - THE FOLLOWING T-ISS-UES - WE-RE -UNR-EMAR-KAB-LE - AT- MI-CROS-COP-IC - EX-AMI-NATI-ON:- - ^ - ---- -- -- -- BRAIN IBRI, COAGULATING GLO ICUI, Lf. TESTIS (TEOP, 111HO (LUI._PROSTATE (PR). SEMINAL VESICLE (SV). URE ----------------------------------------------------------------------------------------------------- to HWI 6329-100 APPENDIX E Quality Assurance Statement Individual Hormone Analysis Data 620 HAZLETC:N W A S H IN G T 0 N t2 VIE 0NtC4A. L t VEIRGSINaIAU OtG 22p I1$K? E TRU&EEUCTCoapCyOMLIaNwSOe,@mdCol"cw"iwv NWI 6329-100 QUALITY ASSURANCE UNIT Project No.: 2200-164 Project Title: rLH: rarid Estradiol: rapid Testosterone Assay Run No.: 17,18,19,20,2 1294,1304 .23.i6.87.68.9o,92.l26,1277,1293, This report has been inspected by the Quality accurately reflects the data generated during study. Assurance Unit and the conduct of the Inspection/Date 2/28/91 4/29,30/91 4/30/91; 5/l/91 5/1,2/91 6/12/91 6/12/91 Findings ReRorted 2/26/91 5/7/91 5/3/91 5/6/91 6/12/91 6/14/91 Auditor D. Bland B. Yeatts B. Yeatts B. Yeatts B. Yeatts B. Yeatts Q%iality Assurfince Unit Christopher m. Lee -7- It,, Date Released u Ph a na s ta Io I - G 0 r m a A y . J a0 7 0 3 8 9 3 -5 A 0 0 F 7 0 3 - 7 5 9 -6 9 F nC0 - V n K 10 s 1 9 9 4 3 6 H A Z L A 6 S V IN A 621 HWI 6329-100 Individual Hormone Data Week 5 Animal Number C67457 C67461 C67464 C67468 C67476 C67491 C67492 C67500 C67502 C67503 Estradiol Apo/mL) <20.0 20.1 <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 Testosterohe (no/mL) 0 ppm T-5180. 77,000 106,000 25,000 48,000 211,0000 45,000 85,000 45,000 19,000 63,000 Luteinizing (nq/mL) @0.4 0.9 0.5 0.6 0.6 0.3 0.3 0.4 0.5 0.3 I ppm T-5180 C67511 <20.0 55,000 0.4 C67514 <20.0 35,000 1.0 C67515 <20.0 45,000 0.7 C67524 20.7 29,0.00 0.5 C67525 <20.0 27,000 0.9 C67544 <20.0 289,000& 1.2 C67550 <20.0 0.4 C67555 <20.0 36,000 0.5 C67558 21.3 64,000 0.7 C67559 <20.0 16,000 0.4 10 ppm T-5180 C67562 0.6 C67568 <20-.0 63,000- 0.6 C67569 <20.0 28,000 0.4 C67574 <20.0 117,000 0.6 C67576 <20.0 60,000 0.5 C76591 <20.0 66,000 0.5 C67592 <20.0 85,000 0.6 C67595 <20.0 47,000 0.5 C67597 <20.0 16,000 1.0 C67601 <20.0 82,000 0.6 a Ressay value. 622 HWI 6329-100 Individual-Homone Data Week-5 Animal Number C67627 C67630 C67632 C67633 C67636 C67640 C67648 C67654 C67659 C67663 Estradiol (Dq/mL) 2-8.8 24.5 21.7 <20.0 <20.0 <20.0 <20.0 32.0 <20.0 Testosterone (na/mL) 30 ppm T-5180 25,000 46,000 82,000 39,000 42,000 16,000 76,000 82,000 43,000 51,000 Luteinizing (nq/mL) 0.6 0.6 0.5 0.4 0.3 0.7 0.6 1.0 0.6 0.4 C67675 C67681 C67682 C67687 C67690 C67696 C67698 C67713 C67715 C67717 24.1 25.7 31.3 46.3 38.8 52.6 36.5 23.1 25.8 32.7 100 pom T-5180 10,000 10,000 45,000 13,000 19,000 24,000 70,000 50,000 18,000 65,000 <0.3 0.4 0.6 0.5 <0.3 0.7 0.4 0.6 0.4 0.6 0 ppm T-5180b C67727 <20.0 7,000 0.3 C67733 <20.0 48,000 0.6 C67735 <20.0 104,000 0.5 C67741 <20.0 40,000 0.7 C67742 22.5 7,000 0.3 C67751 <20.0 63,000 0.7 C67759 <20.0 26,000 0.4 C67761 <20.0 22,000 0.7 C67762 20.1 82,000 0.5 C67769 <20.0 22,000 0.9 b Animals were pair-fed with the high-dose animals throughout treatment. 623 HWI 6329-100 Individual-HormoneData Week 8 Animal Number C67455 C67458 C67463 C67466 C67472 C67478 C67481 C67484 C67495 C67498 Estradiol (pa/mLl <20.0 <20.0 <20.0 22.6 <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 Testasterone ing/mL) 0 opm T-5180 28,300 50,100 149,700 29,800 34,300 4,820 30,700 -32,500 40,700 64,700 Luteinizing (nq/mL) 0.4 0.6 0.4 0.7 0.7 <0.3 0.4 0.5 0.6 0.6 C67512 C67531 C67532 C67539 C67540 C67542 C67545 C67554 C67557 C67560 <20.0 <20.0 <20.0 <20.0 <20.0 <20-.0 <20.0 <20.0 <20.0 I opm T-5180 12,200 227,700' 68,400 33,-100 18,500 19,900 14,700 7,430 73,200 24,000 0.4 0.9 o.5 0.6 0.5 0.7 0.4 0.7 0.5 <0.3 C67570 C67578 C67581 C67582 C67584 C67585 C67586 C67587 C67594 C67614 <20.0 <20.0 <20.0 <20.0 34.2 <20.0 <20.0 <20.0 <20.0 <20.0 10 ppm T-5180 2,840 50,600 44,300 35,300 86,300 24,100 15,900 41,800 22,800 35,900 <0.3 0.5 0.6 0.5 0.5 0.4 <0.3 0.4 0.4 0.3 a Ressay value. 624 HWI 6329-100 Individual'HormoneData Week-8 Animal Number C67617 C67629 C67634 C67639 C67644 C67647 C67656 C67661 C67662 C67667 Estradiol (Dq/mL) 20.4 34.3 23.9 34.1 24.4 <20.0 <20.0 <20.0 <20.0 37.3 Testosterdne (no/mL) 30 Rpm T-5 80 47,900 12,200 38,800 252,000: 286,200 32,300 9,800 14,300 21,400 23,200 Luteinizing (mg/mL) 0.4 0.4 0.4 0.6 0.4 o.5 0.4 0.5 <0.3 0.8 100 ppm T-5180 C67680 <20.0 26,100 0.6 C67699 <20.0 73,900 0.6 C67703 32.4 91,700 0.5 C67708 29.3 25,900 0.7 C67709 <20.0 35,800 0.3 C67710 <20.0 26,600 0.4 C67711 <20.0 62,000 0.4 C67721 32.4 37,000 0.6 C67722 33.7 134,800 0.6 C67724 55.0 43,000 0.5 0 ppm T-51LO-b C67728 <20.0 42,400 0.4 C67729 <20.0 11,500 0.5 C67734 <20.0 23,200 0.3 C67737 <20.0 47,400 <0.3 C67739 20.6 10,700 0.4 C67743 <20.0 18,000 0.3 C67750 <20.0 26,300 0.7 C67752 20.6 10,800 0.5 C67753 20.4 138,800 0.8 C67768 <20.0 15,000 0.8 a Ressay value. b Animalswere pair-fedwith the high-doseanimals throughout treatment. 625 HWI 6329-100 Animal NumLer C67460 C67470 C67473 C67475 C67485 C67487 C67488 C67499 C67504 C67505 C67507 C67519 C67521 C67523 C67533 C67535 C67536 C67549 C67553 C67556 C67563 C67564 C67571 C67596 C67598 C67607 C67608 C67611 C67613 C67615 IndividualHormone Data Week 14 Estradiol (DqlmL) <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 <20.0 Testosterone (no/mL) 0 pt)mT-5180. 32,200 37,200 48,200 7,960 21,200 17,200 24,100 8,820 30,600 18,300 Luteinizing (nq/mL) 0.5 0.4 0.4 0.4 0.4 0.3 0.4 <0.2 0.3 0.3 I Dom T-5180 <20.0 67,200 o.5 <20.0 4,990 0.6 <20.0 23,800 0.3 <20.0 88,000 0.5 <20.0 10.000 0.3 <20.0 24,200 0.4 <20.0 26,400 0.5 <20.0 16,200 0.4 <20.0 63,600 0.5 <20.0 32,100 0.5 10 Dom T-5180 20.4 22,200 0.4 <20.0 30,400 0.3 <20.0 19,000 0.6 <20.0 43,400 0.4 22.2 7,850 0.3 <20.0 50,300 0.6 20.1 16,100 0.6 <20.0 76,400 0.6 <20.0 6,230 o.3 <20.0 42,900 0.3 626 HWI 6329-100 Individual Homone Data Week 14 Animal Number C67616 C67618 C67621 C67623 C67624 C67641 C67643 C67652 C67658 C67668 Estradiol (Dq/mL) <20.0 <20.0 <20.0 23.7 29.6 <20.0 <20.0 <20.0 <20.0 <20.0 Testosterone (no/mL) 30 opm T-5180 18,000 53,100 10,200 13,000 7,470 16,700 51,500 47,400 11,800 6,600 Luteinizing (nq/mL) 0.4 0.3 0.3 0.5 0.8 0.3 0.3 0.5 <0.2 0.7 100 opm T-5180 C67672 <20.0 7,670 0.3 C67673 <20.0 7,420 0.4 C67686 <20.0 29,700 0.5 C67688 <20.0 3,560 1.8 C67691 25.4 23,560 0.5 C67695 31.7 27,800 0.5 C67697 24.6 42,000 0.3 C67700 31.0 2,480 0.5 C67712 <20.0 39,700 0.3 C67720 <20.0 20,300 0.4 0 ppm T-5180b C67732 <20.0 19,200 0.5 C67744 <20.0 58,500 0.6 C67746 <20.0 6,830 0.3 C67747 <20.0 13,200 0.4 C67748 <20.0 12,800 0.7 C67749 <20.0 16,400 0.4 C67754 <20.0 26,800 0.4 C67756 <20.0 5,590 0.3 C67760 <20.0 5,900 0.5 C67767 <20.0 29,400 0.3 b Animals were pair-fed with the high-dose animals throughout treatment. 627 HWI 6329-100 Individual Hormone Data Week 22 Animal Number C67453 C67456 C67483 C67490 C67496 Estradiol (Dq/mL) <20.0 <20.0 <20.0 <20.0 <20.0 Testosterone (nq/mL) 0 ppm T-5180. 7,480 10,800 11,300 8,850 5,140 Luteinizing (no/mL) 0.4 0.4 0.3 0.5 0.4 C67508 C67516 C67534 C67538 C67543 26.0 25.7 34.9 <20.0 <20.0 1 ppm T-5180 21,800 15,500 17,500 23,600 30,000 0.4 <0.3 0.3 0.4 0.5 10 Dom T-5180 C67567 29.8 16,500 0.5 C67575 25.8 3,940 0.7 C67579 22.0 21,000 0.4 C67589 27.7 3,390 0.4 C67593 21.1 14,900 0.5 30 ppm T-5180 C67626 <20.0 17,800 0.5 C67628 21.0 5,830 0.4 C67637 33.5 9,170 1.1 C67664 21.4 20,100 0.3 C67670 <20.0 15,400 0.4 C67674 C67678 C67683 C67694 C67701 <20.0 <20.0 27.1 27.2 23.3 100 Dom T-5180 29,500 8,190 6,130 101,000 34,500 0.7 <0.3 0.4 0.9 0.5 628 HWI 6329-100 APPENDIX F Results of IndividualTest Material Residue Analyses 629 HWI 6329-100 Animal Number C67457 C67461 C67464 C67468 C67476 C67491 C67492 C67500 C67502 C67503 Results of IndividualTest-Material Residue Analyses Week 5 - Kales T-5180 (opml Group I Mean (ppm) a a a a a a C67511 C67SI4 C67515 C67524 C67525 C67544 Group 2 8.33 8.12 6.35 6.87 6.66 6.98 6.81 6.95 6.69 7.79 5.85 5.81 a Below the limit of detection. 8.23 6.61 6.82 6.88 7.24 5.83 630 HWI 6329-100 Animal Number C67550 C67555 C6/'558 C67559 C67562 C67568 C67569 C67574 C67576 C67591 C67592 C67595 C67597 C67601 C67627 C67630 Results of IndividualTest Material Residue Analyses Week 5 - Males T-5180 (ppm) Group 2 (Continued) 7.78 5.88 6.07 11.5 4.46 4.96 Group 3 - 46.4 45.5 54.8 56.2 57.3 57.3 41.2 41.6 54.1 54.5 53.2 53.7 64.6 65.9 65.4 67.2 58.9 59.8 Group 4 Mean (ppm) 5.98 4.71 46.0 55.5 57.3 41.4 54.3 53.5 65.2 66.3 59.3 91.7 92.6 92.2 631 HWI 6329-100 Animal Number C67632 C67633 C67636 C67640 C67648 C67654 r-67659 C67663 Results of Individual Test Material Residue Analyses Week 5 ; Males 7-5180 (DDMI Group 4 (Continued) 99.1 105 97.3 98.7 77.6 78.1 113 101 113 109 124 122 116' 117 89.5 Mean (pami 102 98.0 77.9 107 ill 123 117 - C67675 C67681 C67682 C67687 C67690 C67696 C67698 C67713 C67715 C67717 Group 138 133 151 150 142 137 137 147 113 115 202 211 157 156 159 183 171 163 209 206 136 151 140 142 114 207 157 171 167 208 632 HWI 6329-100 Animal Number C67727 C67733 C67735 C67741 C67742 C67751 C67759 C67761 C67762 C67769 Results of Individual Test Material Residue Analyses Week 5 Males T-5180 (ppm) Grogp 6 Mean (ppm) a a a a a a Below the limit of detection. 633 HWI 6329-100 Animal Number C67455 C67458 C67463 C67466 C67472 C67478 C67481 C67484 C67495 C67498 Results of Individual Test Material Residue Analyses Week 8 z Males T-5180 (ppm) Group I Mean (ppm) a a a a a a' a a C67512 C67531 C67532 C67539 C67540 C67542 C67545 Group 2 6.46 6.97 8.04 8.13 5.26 5.36 6.85 6.77 7.22 7.89 - 6.51 7.55 a Below the limit of detection. 6.72 8.09 5.31 6.81 7.56 7.03 634 HWI 6329-100 Animal Number C67554 C67557 C67560 C67570 C67578 C67581 C67582 C67584 C67585 C67586 C67587 C67594 C67614 C67617 C67629 C67634 Results of Individual Test Material Residue Analyses Week 8 - Males T-5180 (ppm) Group 2 (Continued) 10.1 9.96 7.70 8.07 7.55 7.82 Group 3 60.8 60.3 24.5 24.4 39.0 39.9 42.9 42.5 63.0 62.5 49.3 50.2 24.4 24.9 32.9 36.8 43.7 41.9 73.4 73.4 Group 4 89.3 93.3 105 107 81.7 85.7 Mean (Dom) 10.0 7.89 7.69 60.6 24.5 39.4 42.7 62.7 49.8 24.7 34.8 42.8 73.4 91.3 106 83.7 635 HWI 6329-100 Animal Number C67639 C67644 C67647 C67656 C67661 C67662 C67667 C67680 C67699 C67703 C67708 C67709 C67710 C67711 C67721 C67722 C67724 Results of IndividualTest Material Residue Analyses Week 8 ;.Males T-5180 (ppm) Group 4 (Continued) 105 106 84.5 88.5 49.9 48.5 79.1 82.2 64.3 63.4 60.2 61.6 145' 144 Group 5 138 161 113 112 144 141 83.4 83.4 137 152 173 180 139 135 157 170 155 181 203 218 Mean (ppm) 106 86.5 49.2 80.7 63.9 60.9 145 150 113 143 83.4 145 177 137 164 168 211 636 HWI 6329-IOC Results of IndividualTest Material Residue Analyses Week 8-- Males Animal Numbe C67728 C67729 C67734 C67737 C67739 C67743 C67750 C67752 C67753 C67768 T-5180 (ppm) Group 6 a a a a a a a Mean (ppm) a Below the limit of detection. 637 HWI 6329-100 Results of Individual Test Material Residue Analyses Week 14 Males Animal Number C67460 C67470 C67473 C67475 C67485 C67487 C67488 C67499 C67504 C67505 7-5180 (DDMI Group I a a a a a a a a a a a a a a a Mean (D2m) - C67507 C67519 C67521 C67523 C67533 C67535 C67536 Group 2 7.60 8.45 7.82 8.00 8.09 8.88 6.24 5.65 5.95 5.87 6.71 6.81 7.13 7.38 8.03 7.91 8.49 5.95 5.91 6.76 7.26 a Below the limit of detection. 638 HWI 6329-ICO Animal Number C67549 C67553 C67556 C67563 C67564 C67571 C67596 C67598 C67607 C67608 C67611 C67613 C67615 C67616 C67618 C67621 Results of Individual Test Material Residue Analyses Week 14-- Males T-5180 (ppm) Group 2 (Continued) 5.41 4.84 6.67 7.83 7.99 8.84 Group 3 51.2 49.9 35.6 38.1 32.2 32.8 38.0 37.6 65.0 62.8 21.9 22.2 58.8 60.6 33.9 35.7 39.4 40.3 33.1 34.1 Group 4 68.7 62.4 61.0 64.2 70.0 67.3 Mean (ppm) 5.13 7.25 8.42 50.6 36.9 32.5 37.8 63.9 22.1 59.7 34.8 39.8 33.6 65.5 62.6 68.7 639 HWI 6329-100 Animal Numbe C67623 C67624 C67641 C67643 C67652 C67658 C67668 C67672 C67673 C67686 C67688 C67691 C67695 C67697 C67700 C67712 C67720 Results of Individual Test Material Residue Analyses Week 14-- Males T-5180 (ppm) Group 4 (Continued) 70.0 73.1 109 108 47.6 49.4 57.5 58.4 73.7 73.7 79.5 81.3 64.7 64.7 Group 5 136 133 65.8 66.9 104 102 180 177 167 150 139 149 164 157 166 176 130 129 124 132 Mean (ppm) 71.6 109 48.5 58.0 73.7 80.4 64.7 135 66.4 103 179 159 144 161 171 130 128 640 HWI 6329-100 Results of Individual Test Material Residue Analyses Week 14-- Males Animal Number C67732 C67744 C67746 C67747 C67748 C67749 C67754 C67756 C67760 C67767 T-5180 (ppm) Group 6 a a a a a a a a a a a a a a a a a Mean (ppm) - a Below the limit of detection. 641 NWI 6329-100 Animal Number C67453 C67456 C67483 C67490 C67496 Results of IndividualTest Material Residue Analyses Week 22-- Males T-5180 (ppm) Group I a a a a a a a a. Mean (ppm) C67508 C67516 C67534 C67543 Group 2 a a 1.18 1.20 1.21 a a a a a Below the limitof detection. 642 HWI 6329-100 Animal Number C67567 C67575 C67579 C67589 C67593 Results of Individual Test Material Residue Analyses Week 22 Males T-5180 (ppm) Group 3 0.511 0.462 0.191 222 0a. a 2.59 2.59 Mean (opm) 0.487 0.207 2.59 C67626 C67637 C67664 Group 4 1.52 1.53 2.59 2.60 0.771 1.53 2.60 0.771 C67683 C67694 C67701 Group 5 a a 2.43 2.58 2.41 2.36 a Below the limit of detection. 2.51 2.39 643 HWI 6329-100 APPENDIX G Method Validationfor FC-143 in Feed Method Validationfor T-5180 in Rat Serum 644 MP-APFO-MA PAGE I OF 6 DATE: 01/04/gi REPLACES: original SECTION: 6ooi ASSAY TITLE: AREA OF APPLICABILITY: FC-143 In Rat Feed Hazleton Wisconsin, Inc. Residue Chemistry HWI 6329-100 SCOPE: This method is applicable to the determinationof FC-143 in rat feed samples at levels of I to 100 ppm. PRINCIPLE: FC-143 in the feed sample is extracted Into isooetane/ethermixture under acid conditions, mothylated, and determined by gas chr=atography using an electron capture detector. SENSITIVM: The sensitivityof this method is I ppm of FC-143 in rat feed. PRECISION: The relativestandarddeviation is 21% at I pps (n-5),5.4% at 10 ppm (n-6), 7.7% at 30 ppe (no6), and 3.4% at 100 ppe (nuG). ACCURACY: The average recovery is SS.1% at I ppe (n-6), 99.4% at 10 ppo (n-6), 100% at 30 ppm (no6), and 96.9% at 100 ppe (no6). REFERENCES: Method provided by 3M and modified at Hazleton Wisconsin, Inc. (HWI). HWI Laboratory Notebook Nos. 3418 and 3450. 645 APPROVED BY: Mandl& A. 7shabalala, PhD Staff Scientist Methods Development REVIEWED gy: a@V-L L Deborah L. Keller Manager Quality Assurance Unit (14780) MP-APFO-MA PAGE 2 OF 6 DATE: 01/04/91 REPLACES:- Original SECTION: 6001 HWI 6329-100 DATE: o C) DATE: o 646 MP-APFO-MA PAGE 3 OF 6 DATE: 01104/91 REPLACES: Original SECTION: 6001 HWI 6329-100 SAFETY PRECAUTIONS: Observe all standard laboratory safety procedures as outlined In the HWI Safety Training Manual. FUNMENTAL EQUATIONS: FC-143 (Amwnium perfluarooctanato) formula: CF,(CF.),,COD-NH+, Nonadecanuorodoc&nGiC acid (NDFOA) formula: CF3(CF,,),Coom INTERFERENCES: There say be an occasional interferencepeak that is observed at the I ppm level. When it does occur, that sample must be reassayed. QUALITY ASSURANCE: Two standard curves, low mW high, each consistingof a minimum of four points, are used for calculation. APPARATUS: 0 0 0 0 0 0 a a EM: Voluntric pipettes,various sizes Voluntric flasks,various sizes Eppendorfdigital pip*ttes,variloussizes Disposablescintillationvials,'20aL Horizontal shaker, EWrbach Corporation Gas chromatograph with electron capture detector, MP 5890A, Hewlett Packard Autainj*ctor, MP 7673, Hewlett Packard Integrator, MP 3396A, Hewlett Packard Equivalent equipmentmay be substituted. REAGENTS: 0 a 0 a 0 .0 HM: Milli-Q\water Isooctue, high-puritysolvent,8 & J Ethyl other, high-pur,itsyolvent,8 & J Pombloric acid, concentrated,ACS reagent grade, Aldrich Hydrochloric acid, concentrated, GR, EN Science ciazomethans solution in other, prepared according to procedure described in Aldrich Technical InformationBulletin No. AL-113. Equivalentreagentsmay be substituted. 647 MP-APFO-MA PAGE 4 OF 6 DATE: 01/04/91 REPLACES: Original SECTION: *6001 HWI 6329-100 TEST MATERIALS:. 0 FC-143, Lot No. 115, purity 98%, 3H 0 Internalstandard, (NDFOA),Lot No. IIZOU, purity 98%, Aldrich PROCEDURE: Preparati-onof Stock Standard Solutions Solution A. FC-143-t2C ae/mLi. Accurately woigh about 20 og of FC-143 into a 1000-nLvolumtric flask. Dissolve the solutionand dilute It to volume with water. Star* it at room temperaturein a polyethylenebottle. Prepare fresh solution every 6 months. Solution B- FC-143 (2,000 no/mLl Accurately weigh about 200 mg of FC-143 into a 100-nL volumetric flask. Dissol;othe solutionand dilute it to volume with water. Store it at room temperaturein a polyethylenebattle. Prepare fresh solution every 6 months. Solution-C.FC-143 (800 Ug/mL. Using a volmtric pipette, transfer 20 mL of Solution9 to a 50-mLvoluntric flask. Dilute it to volume with water. Mix the solutionthoroughlyand transferIt to a polyethylenebattle. Store it at roas temperature. Prepare fresh solution as needed. Solution 0. NOEM (1.5 =/mL). Accuratelyweigh about 30 mg of NOFOA into a 20-sL volumtric flask. Dissolveit in 90:10 v/v isooctano-othylother. Add 100 ItLof parchloricacid vW dilute the solutionto volume with isooctano-othyl . ether. Store it at room temperatureand prepare fresh solution every 6 months. SglutionE. NDFDA (13 no/mL).' Using a voluntric pipette, transfer 10 mL of Solution0 to a 1,000-uLval@tric flask. Dilute it to volume with isooctantethyl other. Mix the solutionthoroughlywW store it at room temperature. Prepare fresh solution as new*d. Preparation of Workina Solutions Calibrationstandards. Followingthe scheme below, transfer aliquots of the appropriatestock solutionsto separatescintillationvials containing 1 9 of control rat food, 4 mL of water, and 2 mL of hydrochloricacid. Mix the solutionsthoroughly on a vortex mixer. 648 MP-APFO-MA PAGE 5 OF 6 DATE: 01/04/91 REPLACES: Original SECTION: .6001- NWI 6329-100 Solution Volume, aL Final Conc@@ Dom A A so A 100 A 200 A 400 A 600 c 25 c so c is c 100 c ISO 1.0 2.0 4.0 8.0 12.0 20.0 40.0 60.0 $0.0 120 Internalstandardtxtractint(1.6maiall. using a volumetricpipette,transfer 100 aL of SolutionE to I-Lvoluntric flask. DiluteIt to volumewith Isoactanso-ther. star*the solutionat roomtnporature and preparefresh solutionevery 6 months. Sawle Pronaration weigh a 1-g few sampleinto a 20-mLdisposablescintillationvial. Add 4 mL of water and 2 aL of hydrochloricacid. Mix the sm*le thoroughlyon a vortex mixer. SAMIO Extraction To each of the vialscontainingthe standaks and samples,add 5 aL of internal standardextractant. Cap the vials tightlyand shake for 2 hours at high speed an a horizontalshaker. Centrifuge&M transferI-nL aliquatsof the organic layer to separateautoinjoctorvials. Add 0.5 aL of the diazomthant solution into each of the vials. Cap the vialswW six the su*les on a vortexmixer for 10 to 15 seconds. lfkjoc2t ML of each sm*le intothe GC system. Chromlt2graphicCondition2 Colum: S% phonylmothyl silicon*,0.31 po i.d.x 50m, 1.03 go film, HewlettPackard,Colum No. 442-85-04 lnj*ctionpart linor: Splitlessglassliner Purge time, minutes: ON a 1; OFF o 38 Carrier gas: 5% uthans/95% argon Flow rate: I mL/minuto Nake-up gas: 5% uthant/9S% argon Flow rate: 36 mL/minuto InJettionport tevoraturs: 2509C Detectorte"araturs: 250*C 649 MP-APFO-MA PAGE 6 OF 6 DATE: 01/04/91 REPLACES:. Original SECTION: 6001 HWI 6329-100 Temperature program: initialoven temperature: Initialtime R&M A: Final temperature: Ru* 9: Final temperature: Final time: lnjtctionsize: 40-C 10 minutes S*C/minuto 1600C 25*C/minuto 230*C 8 minutes 2 AL ftg": All conditionsmay be adjust#d to optimizechrmatography. CALCULATIONS: Compute the linear regressionequation relatingthe peak height ratios of the calibrationstandardsto their respectiveconcentrationsin the food. Using the peak height ratio of the sample food in the appropriatelinear regression equation, compute the dos* concentration in pps as follows: ppm FC-143 o (y -b)/s x F where y a peak height ratio of the sample faW b a intercept from the appropriatelinear regression equation 0 a, slope from the appropriatelinear regression equation (1/ppm) F a calculationfactor (1/6) *rG a weight of sample fe" measuredout for extraction(g) Example: For a I ppe sample, y a 0.159 b n 0.08825 a - O.OM 9 - 1.0222 ppo FC-143 - (0.159 - O.CSU )/0.0646 x I a 0.810 1.0222 Average % recovery a (Averagemunt found/amunt claiud) x 100 Exw*le: For a I-ppe sample, average % recovery - (0.851/1.00)x 100 650 MP-APFS-MA PAGE I OF 7 DATE: 03/05/92 REPLACES: Original SECTION: 6011 NWI 6329-100 ASSAY TITLE: AREA OF APPLICABILITY: FC-143 in Rat Serum Hazleton Wisconsin, Inc. Residue Chemistry SCOPE: This method is applicable to the determination of FC-143 in rat serum samples at levels of 1.0 ug/mL to 12.7 ug/mL. PRINCIPLE: FC-143 in the serum sample is extracted into isooctane:ethylether mixture under acid conditions, methylated, and determined by gas chromatography using an electron capture detector. SENSITIVITY: Sensitivitydescribedas the lowest fortificationlevel that was validated,is 0.7 ug/mL of FC-143 in rat serum. PRECISION: The within day standard deviation (n-3) for..FC-143in rat serum is 0.3% at 0.794 .ug/mL,2.7% at 1.56 jig/mL,1.7% at 3.18 Ag/mL, 0.7% at 4.76 ug/mL, 0.9% at 6.35 ug/mL, 2.9% at 7.94 jig/mL,0.9% at 9.53 ug/mL, and 0.4% at 12.7 Ag/mL. The between days standard deviation (n-6) for FC-143 in rat serum is 10.1% at 1.56 Ag/mL, 10.8% at 3.18 pg/mL, 10.2% at 4.76 Ag/mL, 10.2% at 6.35 ug/mL, 10.1% at 7.94 ug/mL, 9.5% at 9.53 pg/mL, and 8.4% at 12.7 ug/mL. ACCURACY: The average recovery is 99.9% at 0.794 ug/mL (n-4), 99.9% at 7.99 ug/mL (n-S), and 97.3% at 149 ug/mL (n-S). REFERENCES: Method provided by 3M and modified at Hazleton Wisconsin, Inc. (HWI). HWI Laboratory Notebook Nos. 3418 and 3450. 651 MP-APFS-MA PAGE 2 OF 7 DATE: 03/05/92 REPLACES: Original SECTION: 6011 HWI 6329-100 APPROVED BY: Mandla A. Tshabalala,PhD Staff Scientist Methods Development DATE: REVIEWED BY: Shh;rrr@yRyLW.'W.-Petsel anager - ipoauality AssuranceUnit DATE: L 6S2 MP-APFS-MA PAGE 3 OF 7 DATE: 03/05/92 REPLACES: Original SECTION: 6011 HWI 6329-100 SAFETY PRECAUTIONS: Observe all standard laboratory safety procedures as outlined in the Hazleton Wisconsin Safety Training Manual. INTERFERENCES: There may be an endogenous interferencepeak that elutes around the retention time for PC-143. This may be overcome by appropriate dilution or be dampened with control blank serum. QUALITY ASSURANCE: A standard curve consistingof a minimum of five points is used for calculation. APPARATUS: 0 Volumetric pipettes, various sizes a Volumetric flasks, various sizes 0 Eppendorfdigital pipettes, various sizes 0 Disposable 12 x 75 m polypropylenetest tube (with cap) 0 Horizontal shaker, Eberbach Corporation 0 Gas chromatograph with electron capture detector, HP 5890A, Hewlett Packard 0 Autoinjector, HP 7673, Hewlett Packard 0 Integrator, HP 3396A, Hewlett Packard Note: Equivalent equipment may be substituted. REAGENTS: 0 Milli-Q wattr 0 Isooctane, high-purity solvent, Burdick & Jackson 0 Ethyl ether, high-puritysolvent, Burdick & Jackson 0 Perchloric acid, concentrated,ACS reagent grade, Aldrich 0 Hydrochloric acid, concentrated, GR, EM Science a Diazomethane solution in ether, prepared according to procedure described in Aldrich Technical InformationBulletin No. AL-113. h=: Equivalentreagentsmay be substituted. 653 MP-APFS-MA PAGE 4 OF 7. DATE: 03/05/92 REPLACES: Original SECTION: 6011 HWI 6329-100 TEST MATERIALS: 0 FC-143, purity 98%, 3M 0 Internalstandard,(NDFDA),purity 98%, Aldrich PROCEDURE: Preparationof Stock StandardSolutions Solution0. FC-143 (320 ualml). Weigh 16 mg of FC-143 into a 50-mL volumetric flask. Dilute it to volumewith water. Store at room temperaturein a polypropylenecentrifugetub*. Preparefresh solutionevery 6 months. Solution H. NDFOA (1.7 ma/mL). Weigh 43 mg of NDFDA into a 25-mL volumetric flask. Dissolve it in isooctane:ethylether (90:10,v/v). Add 100 AL of perchloricacid and dilutethe solutionto volumewith isooctane:ethylether. ether. Store at room temperatureand.prepare fresh solution every 6 months. Preparationof Working Solution InternilStandardExtractant(1.6 ug/mLl. Usinga volumetricpipette,transfer 1.0 mL of SolutionN to a I-L volumetricflask. Diluteit to volumewith isooctane:ethylother. Store the solutionat room temperatureand preparefresh solutionevery 6 months. FC-143 StandardSolution. Followingthe scheme below,transfer aliquotsof the appropriatestock solutionto separate25-mL volumetricflasks. Dilute to volume with Milli-Qwater. Store at room temperaturein polypropylenecentrifugetubes. Prepare fresh solutionas needed. Standard Number P-1 P-2 P-3 P-4 P-5 Stock Solution Concentration (ug/mL) 320 320 320 320 320 Aliquot Volume (mL) 10 a 7 6 4 Dilution Volume (mL) 25 25 25 25 25 Final Concentration (ug/mL) 128 102 89.6 76.8 51.2 654 MP-APFS-MA PAGE 5 OF 7 DATE: 03/05/92 REPLACES: Original SECTION: 6011 HWI 6329-100 Standard Number Stock Solution Concentration Aliquot tug/mL) Volume (mL) Dilution Volume (mL) Final Concentration (ug/mL) P-6 320 3 25 P-7 320 2 25 P-8 320 1 25 P-9 320 0.5 25 38.4 25.6 12.8 6.40 Preparationof Matrix Standard Curve Pipette a 100-1&Lcontrol serum into a 12 x 75 mm disposable polypropylenetest tube (withcap). Fortifywith 10 uL of standardsolutionand mix. Add 100 ;ILof Milli-Qwater and 50 aL perchloricacid and mix. Sample Preparatign Pipette a 100-1&Lserum sample into a-12 x 75 m, disposablepolypropylenetest tube (withcap). Add 110 #L of Milli-Qwater and 50 1&LPerchloricacid. Mix the sample. Note: For samples containinglevels of FC-143 which exceed the standardcurve, preparea dilutionof the originalserumwith controlserum. The total volume of the matrix diluted sample taken for analysis should be 100 AL. Sample Extraction 1. To each of the test tubes containingthe standards and samples,add 2 mL of internalstandardextractant,cap the test tube, and vortexvigorously(on a multiplevortexer)for S minutes. 2. Placethe tubes in a horizontalpositionin a horizontalshaker. 3. Shake at high speed for 30 minutes. 4. Return the tubes to a test tube rack and allow to stand on a lab bench until the layersseparate. S. Transfer1-mL aliquotsof the organiclayer (top layer)to separate autoinjectorvials. 655 MP-APFS-MA PAGE 6 OF 7 DATE: 03/05/92 REPLACES: Original SECTION: 6011 HWI 6329-100 6. Add 0.25 mL of diazomethanesolutioninto each of the vials. 7. Cap the vials and mix the samples in a vortexmixer for 5 to 10 seconds. S. Inject 2 ;&Lof each sample into the gas chromatography(GC) system. Chromalographic Conditions Column: 5% phenyl methyl s11icone, 0.31 I;m i.d. x 50 m, 1.03 pm film, Hewlett Packard Injectionport liner: Splitlessglass liner Purge time, minutes: ON - 1; OFF - 10 Carrier gas: 5% methane/95% argon Flow rate: Makeup gas: I mL/minute 5% methane/95% argon Flow rate: 35 mL/minute Injectionport temperature: 250*C Detector temperature: 310*C Temperature program: Initialoven temperature: 70*C Initialtime,. 13 minutes Ramp A: 5OC/minute Final temperature: 95*C Ramp 8: 25*C/minute Final temperature 230*C Final time: 10 minutes In.4,ectiosnize: 2 #L Note: All conditionsmay be adjusted to optimizechromatography. CALCULATIONS: Computethe linear regressionequationrelatingthe peak height ratios of the calibrationstandardsto their respectiveconcentrationsin the serum. Using the peak height ratio of the sample serum in the appropriatelinear regression equation, compute the dose concentrationin ppm as follows: 656 MP-APFS-MA PAGE 7 OF 7 DATE: 03/05/92 REPLACES: Original SECTION: 6011 HWI 6329-100 ppm FC-143 (y b)/(mx F) where: y s Peak height ratio of the sample serum b - Interceptfrom the appropriatelinear regression equation m - Slope from the appropriate linear regression equation F - Calculationfactor (100/V) V a Volume of sample serum measured out for extraction (AL) 657