Document EvQaj8664MK29xqGyoy040Q0R
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Study Tit10 Acute Toxicity Of N2803-2 to t b Fathead ILIMw, RLnephleo p z 0 ~ 1 a .
Data Requiromnt: Quideline N u m b e r U.S. EPA-TSCA, m i d e l h e 797.1400
Authora Timothy J. Ward Peter L. Karalski Robert L. Boeri
November 16, 1995
I
Sponsor 3n Ccmmmpany Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Winnesota 55144
TISkhg lrrcilftr T.R. Wilbury Latoratoriea, Inc.
40 Doak8 Lane Narblehead, Masrrachuaetts 01945
T.R. Hilbury study Number 888-TH
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T h i s atudy was conductod according to CpA Gooel Laboratory Practice Regulations ( 4 0 CIR 7 9 2 ) , with t h e fol1.owing exception. The s t a b i l i t y of tho t e a t substance wn8 8aS-d but not v o r i f i o d a t T.R. wilbury Laboratories. Deviationm from t h e atutiy protocol and protocol munrlments u e presented i n Section X I 1 of this report.
T.R. wilbury Study Number 888-TB
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8ubpitt.d by: T.R. Wilbury Laboratories, Inc. 40 Doakm Lane Uarblehead, tfassachusett:m
Certification:
T.R. Wilbuq Laboratories, Inc. sttidy number 888-Tl3, Acute Toxicity of N2803-2 to thu rathead minnow, Pfmephales promelas, was audited by the Quality Assurance Unit for compliance with the protocol, atandard operating procedurer, and applicable Good Laboratory Practices (V.8. EPA, 1993). Quality assurance audits wera performed and the findings reported to T.R. Wilbury Laboratories management and the study director on the following dates;
Aspect Audited
Protocol: In-life; Draft Report: Final Report:
Audit Date
8/ 3/95 9/12/95 9/22/95 11/16/95
Reported to Study Director
0/ 4/95 9/12/!>5 9/22/!)5 11/16/!)5
Reported to
Hanagement
0/ 4/95 11/16/95 11/16/95 11/16/95
Quality Amrrurance Officer
T.R. Wilbury study Number 888-TH
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2 3 4 5 6 7 8 8 13 17 18 19 20
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Table 1. Table 2. Table 3. Table 4. Tabla A.1. Figure 1.
Chemical characterization of roprementatioe sample of d i l u t i o n water wed for t h e t o x i c i t y t e a t v i t h f6thead Ltinnova, Pimephalos promelas, and N2803-2
Test system summary f o r t h e t o x i c i t y t e n t v i t h N2803-2 and fathead dnnWD, Phephales promelas
survival and sublethal e f f e c t data from the t o x i c i t y t e s t with fathead minnow., P h ~ p h a l e s promelas, And N2803-2
Median l e t h a l concentration. (LC50a) f r m t h e t o x i c i t y t e s t with fathead minnowa, Pimaphales promelas, and N2803-2
Diasolved oxygen concentration, conductivity, p ~ , and temperature measured during t h e t o x i c i t y t e n t with fathead minnows, Pimephales promelas, and N2803-2
s u r v i v a l of f a t h e a d minnowti, Pimephales promelas, exposed t o N2003-2 f o r 96 hours
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T.R. Hilbury Study Number 888-TH
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~ h oacuto toxicity of ~2803-2to tho fathead minnow, Pfmephalea promeha, i8 describod in thi8 final report. Tho tout we8 conducted for 3x Company for 96 hours from septembor 8 to 12, 1995, at T.R. nilbury Laboratorier, Inc. in Marblehead, Ua88achUsott8. If was conductod by Potor L. Kovalskf, Jeanne UAgazu, Jacquelin0 NeViU8, M v e n 8toVon8, Potor Uovium, Robert Bo.ri, and T h t h y Ward according to the protocol dovoloped for T.R. Wilbury Study Numbor 888-TH. N2803-2 18.8 8upplied by tho sponsor.
The test was performed under 8tatic condition8 with five concentration8 of test substance and a dilution water control at a temperature of 21.6 to 22.0.C. The dilution water wan filtered deionized water collected at Marblehead, Hassachusett8 and adjusted to a hardness of 40 mg/L as CaCO,. Aeration was initiated at 72 hours to maintain dissolved oxygen concentrations above an acceptab1.e level.
Nsminal concentrations of N2803-2 were 0 (control), 160, 250, 400, 630, and 1,000 mg/L. Fathead minnow3 used in the test: were procured from a commercial supplier and acclimated to test conditions at T.R. Wilbur1 Laboratories. After 96 hours of exposurm the control fish had an average
wet weight (blotted dry) of 0.36 g and an average tota.1 length of 34.9 m.
All fish were i n good condition at the beginning of the study. Exposure of fathead minnows to the test substance resulted in A
96 hour median lethal concentration (LC50) of 960 mg/L N2803-2, with a 95% confidence interval of 830 to >1,000 mg/L8. The 96 hour no observed effect concentration (NOPIC) is 630 mg/L.
T.R. nilbury Study Number 888-TH
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V I . 0-
IIWOIIIATION
Material Teated:
N2003-2
sponsor :
3U company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 S t . Paul, Uinnesota 55144
I Test substance 3n company
shipped ?rem
Environmental Laboratory
367 Grove Street
Building 53-5s-02
St. Paul, Minnesota 55101
Reported Purityr
100%
study Initiation Date:
Experimental Start Dater
Experfmental Completion Dater
study Completion Dater
Archive Location for the Biological Raw Data and a copy of the Final Report r
August 16, 1995 (protocol signed) September 8, 1995 September 12, 1995 November 16, 1995
T.R. Wilbury Laboratories, Inc. Uarblehead, Uassachusetts
T.R. Wilbury study Number 800-TH
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VII. L*TM)WcTIOW
Thin study wan sponsorod by 31 Company, st Paul, WLnnoaota. Tho
objective of the study WAS to d e t e d n e tho 24, 48, 72, .ad 96 hour rudian lothal concontrationa (LC50r) of tho teat aubrtanco to tho fathoad minnow, Pimophalos promelaB, a froshwclter fiahr under Statio conditioo.. Tho teat protocol wan baaed on U.8. EPA wthods (U.S. EPA, 1993). Tho report contain. aoctfona that demcribe tho mothode and materials employod in tho study, and result6 of the invertigation. The report also contains an
appondix that presents water quality data collected during the teat.
"8ST SUBSTAWS:
The sample of N2803-2 (T.R. wilbury Laboratoriea fi~mpleNumber 529) was delivered to T.R. Wilbury Laboratorios on August 29, 1995 in a 5 gallon metal CAD within a Cardboard box At ambient t.emperature. The test substance was contained in an approximately 150 d plastic jar. The
attached label included the following information: -N2803-2, s. Beach, 2-
JE-09, (612) 118-7452, 8/22/95'.
N2803-2 (a clear liquid) was supplied by 31 Company, st Paul, ninnesota. Prior to use the test substance was storod in the dark at room
temperature. Test Substance stock solutions were formulated in dilution water without the use of a solvent. The test aubstance wan aaaumed to
have a purity of 100% active ingredient and to be stable under storage and tenting conditions. Unused test subntance will be returned to the sponsor. ,
DILUTIOB WATER:
Water used for acclimation of test organisms and for all toxicity testing was deionized water collected at T.R. Wflbury Laboratories in Marblehead, mssachusetts. Water was adjusted to a iaardnebs of 40-48 mg/L AS CACO, and stored in 500-gallon polyethylene tanks where it WAS aerated and continuously passed through a particle filter, ultraviolet sterilizer, and activated carbon. Results of chemical analysis of a representative sample of water are presented in Table 1.
Juvenile fathead minnows, Pixnephales promelar, employed as test organism were from a ringlo source and wero procured from Aquatic Biosyatems, rortcollins, Colorado onAugust10, 1995. The Species of the fish WAS verified uaing an appropriate taxonomic key.
T.R. Wilbury study Number 888-TH
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T a l e 1.
Chemical charaaterization of a representative sample of dilution wator uaed for tho toxicity tost with fathoad minnow#, Pinmphales promelas, and N2803-2.
~sranmtor~
Unit of Heasuremont
Detection Limit
~-
Ifearnured Valum
notal. AlUdnUUI Arsenic Boron cadmium Chromium Cobalt copper Iron Lead Xercury Nickel silver zinc
Nitrate Chloride Fluoride Total organic carbon Total Phosphorus
Organochlorine Pesticides
Toxaphene
Organophosphorus Peaticidea
Dimethoate TEPP , nonocrotophos
0.1 0.01 0.5 0.0002 0.01 0.03
0.005 0.03 0.005 0.0003 0.03 0.02 0.02
0.05 1 0.1
1 0.03
0.5 2
0.5 2.0 2.0 2.0
NDa ID ND ND ND UD ND 0.03 ND ND ND ND ND
ND
ND
ND
ND
ND ND
ND
ND
ND
ND
Notomr
$J
1. notala, nitrat., chlorido, fluoride, total organic carbon, total phosphoruo, organochlorine pesticides, organophosphorus peaticidea, and PCBS were measured in deionized water (uaed to formulato dilution water) that waa collected during August, 1995 and analyzed by Pace, Inc. as part of routine water quality te#ting,
2. ND = not dotected at or abovo the detection limit.
T.R. Wilbury study Number 888-TH
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Prior to teathg the fiah woro ~ i n t a i n o d under flow through condition# in a 270 liter fiberglaaa tank or a 40 litor glaaa aquarium. During acclimation fiah were not treated for diaoaso, thoy wero f r w of apparent aickneaa, injurier, and abnormalities at the boginning of tho toat, and mortality during the final 48 houra of . c c X h t f o n wa8 lea# than 3*.
During the 14 day period h f o r o the ntakt of th. teat the acclimation temperature range was 21.0 to 22.3.C and the diaaolved oxygen concentration waa alwayn at leaat 8.6 mg/L. During acclimation fiah were fed a dry comercia1 food (Trtra nine Staple mod, Lot I T n O 5 ) daily except during the 48 hours immediately preceding the test. ?iah were not fed during the teat. At the conclunion of thm k a t the control finh had an average total length of 34.9 mm and an averago wot weight of 0.36 g (loading during the definitive toxicity test waa 0.24 g/L).
TOIICITI TESTIlDOl
A static screening test was conducted from August 18 to 22, 1995 with five concentration# of test aubatance and a dil.ution water control. Nominal concentrations of N2803-2 were 0.10, 1.0, 10, 100, and 1,000 mg,L. After 96 hours of exposure there was 1008 survival in the control and at 0.10, 1.0, 10, and 100 mg/L, and 0% survival at 1,000 mg/L.
The definitive toxicity test was performed from September 8 to 12,
1995, according to T.R. Wilbury Protocol 888-TB (Acute Toxicity of U2803-2
To The lathead BLhnow, Pfmephales p r o m e l a s ) . The protocol, which was
signed by the atudy director on August 16, 1995, is based on procedures of
the U.S. Environmental Protection Agency (1993). A
of the test
ayntem is preeented in Table 2.
The test was conducted at a target temperature of 22 t 2% with five concentrations of test substance and a dilution water control. Nominal concentrations of the test substance were: 0 mg/L (control), 160, 250, 400, 630, and 1,000 mg/L. Appropriate amounts of test wbstance were added directly to dilution water in test vessels to formulate test media without the use of a solvent.
Twenty fatheadminnows were indiscriminately and equally distributed among t w o r e p l i c a t o m of each t r e a t m e n t . The t e n t wam performed in
20 liter glasa aquaria that contained 15 litera of feat aolution (water depth waa approxhately 18 a).Test veseela were randomly arranged in a
water bath during tho 96 hour test (a random numbera table wan used to select tho location of each vessel) and they were loorely covered. A 16 hour light and 9 hour dark photoperiod with a 15 minute traneition period
was automatically maintained with cwl-white fluoreacent light. that
provided a light intensity of 25 footcandlea. Aeration was initiated after the 72 hour dissolved oxygen concentration lae&airrements t o maintain dinsolved oxygen concentrations above acceptable levdn.
T.R. nilbury study Number 888-TH
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Tab10 2.
Temt symtem muaQuy for t h o t o x i c i t y t o r t with N2803-2 and fathoad alnnw8, PimephJe# promela#.
specion: Ago/Sizo of Tent organism: Test Duration: Temt V e s m e l s r Depth of T e s t Medias Number of R e p l i c a t e T e s t Ves8elsr organisms per Replicate: Temperature Ranger Minimum Dissolved oxygen: Photoperiod: Light Intensity: water Q u a l i t y MeasureUSintS:
End Points:
Acceptability criteria:
Pimphales promolaw
Juvenile8/-0.36 gram, wet weight
96 hours
20 l i t e r glass a q u a r i a
I8 c m
2
10
21.6-22.O.C
4.6 mg/L ( p r i o r t o a e r a t i o n )
16 hours l i g h t and 8 hours dark with a 15 minute transitio:! period.
25 footcandles
Dimnolved oxygen, pB, temperature, and conductivity d a i l y i n each test venoel. Tsmperaturemoaouredat l e a s t ovory hour i n 1 t e n t vessel. nardnens, alkalinity, acidity, and p a r t i c u l a t e
mutter and TOC in t h e d i l u t i o n water. Dilution water a n a l y s i s in summarized in Table 1.
. 24, 48,
NOLC
72,
and
96
hour
Lc50;
96
hour
slot control mortality and acceptable
temperature range.
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T.R. wilbury Study N u m b o r 888-TX
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The number of rurviving orpanirmr and the occur~oncoof rublethal
offocta. (hmobiliration, lomr of equilibrium, lomr of roflox,
excitability, diBCOlOratiOn, or change in behavior1 wore d e t e d n o d
ViBUally and recorded initially and aftor 24, 48, 72, and 96 bourn
(mortality and rublotha1 offoctm bmro monitored twice oach day). m a d
t e s t organism wore removed when first obnervod. Dimsolved oxygen ('I51
xodol 57 metort inrtrunwnt numbers I), pH (Bockman mod01 pa1 12 motor;
i n n t m w n t number 124), conductivity (Colo P-r
mater,,instrument number
94), and temperature (Beckman model pH1 12 meter; instrument numbor 124,
probe pHT-1) wero measurod and recorded daily in each temt chamhor that
contained live a n h l m . The temprrature in one test vessel warn rrcordod
at least hourly during the teat.
STATISTICAL WlITBODSt
Result. of the toxicity test were interprated by standard etatietical techniquee. Computer methods (Stephan, 1983) were used to calculate the median lethal concentrations (LCSOS). All calculations were performed by the author using nominal concentrations of test substance and the number of live fathead minnows. The no observed effect concentration is the highemt concentration of test substance that did not cause any toxicant-related mortality or sublethal effects.
?.a. wilbury study Nunrber 888-TH
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Inmoluble m a t o r i a l yam not noted during t h o tost. B i o l o g i c a l and
water q u a l i t y d a t a generated by t h o a c u t e t o x i c i t y test a r o premonted in
Table 3 and Appendix A, respectivoly. Tho wamurod concentrations of
d h r o l v o d oxygon ranged from 4.6 t o 0.0 mg/L ( a e r a t i o n war f n t i a t o d a f t e r
i
t h o 72 hour dismolved oxygen doterminationm), t h o temperature ranged from
21.6 t o 22.0.C, conductivity ranged from 140 t o 180 pmhos/cm, and pa
. ranged from 7.4 t o 7.8. Ora hundrod percent s u r v i v a l occurred in t h o
control exponura nnd theme fathead minnow did not oxhibit any Dublotha1 effects
The 2 4 , 40, 72, and 96 hour LC50s f o r fathead minnows expored t o N2803-2 a r e presented in Table 4. The 96 hour X 5 0 f o r f a t h e a d minnows
exposed t o N2803-2 is 960 mg/L, with a 9 5 t confidence i n t e r v a l of 830 t o r 1 , O O O rng/L. The elope of t h o 96 hour dose respanse curve is 7.3. Tho 96 hour no observed e f f e c t concontration fNOEC) is 630 mah.
T . R . Wilbury Study Number 888-TH
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~ a b l o3 .
Survival and mublothal o f f o c t datm from tho toxicity t o s t with fathoad minnw8, Plpepha1.m promolam, and 12803-2.
Nominal Concentration
of N2803-2 (WL)
Rep.
Number alive 0 24 4 8 72 96 hr hr hr hr hr
Umber affoctod
0 24 48 72 96
hr hr br hr hr
0 (control) 160 250 400 630 1,000
1
10 10 10 10 10
2
10 10 10 10 10
1
10 10 10 10 10
2
10 10 10 10 10
1
10 10 10 10 10
2
10 10 10 10 10
1
10 1 0 10 10 10
2
10 10 10 10 10
1
10 10 10 10 LO
2
10 10 10 10 8
1106433
2
108766
00000 00000
00000 00000
00000 00000
00000 00000
00000 00000
00000 00000
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L_ . "'.l
...
1 a
.I
160
250
430
630
1000
Tesi Substonce (mg/L)
Figure 1. survival of fathead minnows, Pimephales promelas, exposed to N2803-2 for 96 houra.
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Tablo 4.
mdimn l o t h a l concontrationr (LCSOr) from tho t o x i c i t y t o r t with fathead minnow, Pimwphalws p r o a d a s , and N2803-2.
Expornur. period
LC50
95 porcent confidenco limit
Calculation mothod
24 hour8
~ 1 , 0 0 0mg/L
--
--
72 hours 96 hours
970 mg/L 960 mg/L
630 to >1,000 mg/L 830 to >1,000 Ag/L
Binomial Probit
T.R. Wilbuw Study Number 888-Tl
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ABm. 1986. Standard P r a c t i c e for Conducting Acute Toxicity T o r t s w i t h
l h h e l , mcroinvsrtebrates, and ~mphibianr. E-729-80.
Annual
Book of Standards.
s t e p b n , C.E. 1983. computer Program f o r t h e c a l c u l a t i , o n o f u150 Va1U.B.
U . 8 . EPA. Duluth, mi. Perronal Comunicatfon.
U.S. EPA. 1993. Toxic Substances c o n t r o l A c t Teat Guidelines; F i n a l Rules. section 797.1400.
U.S. EPA. 1993. 4 0 CPR P a r t 792. Toxic substances c o n t r o l A c t (TSCA); oood Laboratory P r a c t i c e standards. Code of Federal Regulations, T i t l e 40, July 1, 1993.
T.R. W i l b w s t u d y N u m b e r 88B-TII
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X I , IlIOrnntRI PAOZ
Study -Director hqustic TOXiCOlOgist Aquatic Toxicologist Biologist Biologist Biolbgist
Coauthor
T.R. wilbury study Number 888-Tn
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IY
jy
tone ST TERY Te someon ot Sm fen,ny bite 10Ter ilms
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Appendix A. U A T m QUALITY DATA ?Ron TQIICtTI TEST
T.R. Wilbuq Study Number 888-TH
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Tablo A.1.
Diooolved oxygon concentration, conductivity, ptf, and temporature moaourod during t h e t o x i c i t y t o a t with f a t h e a d minnow., Pinrephale8 promoha, and 12803-2.
Nominal Concontration
of N2803-2 (mg/L)
Rep.
Diooolvod Oxygon (mg/L)
0 24 48 72 hr 96 hr hr br b a hr
-Conductivity (pahoo/cm)
0 24 48 72 96 hr hr hr hr hr
0 (control) 1 2
160
1
2
250
1
2
400
1
2
630
1
2
1,000
1
2
8.4 7.1 6.6 6.3 7.1 8.6 8.4 7.2 6.7 6.3 7.2 8.6
8.4 7.0 6.6 6.2 7.0 8.4 8.4 6.9 6.6 6.3 7.2 8.7
8.4 6.9 6.5 4.9 6.8 8.8 8.4 6.9 6.4 5.8 7.0 8.5
8.4 6.7 6.2 5.2 6.6 8.3 8.4 6.8 6.2 5.3 6.9 8.6
8.4 6.6 5.8 4.6 6.8 8.6 8.4 6.6 5.8 4.7 7.1 7.8
8.4 6.8 5.6 4.8 6.7 8.0 8.4 6.7 5.5 4.6 6.9 8.4
150 140 150 150 150 150 150 150 150 150
150 140 150 150 150 150 140 150 150 150
150 150 150 150 150 150 150 150 150 150
150 150 150 150 160 150 150 150 150 160
160 150 150 150 160 160 160 160 150 170
170 170 170 170 180 170 170 170 170 180
Noter
a1 t e s t veaaela were a e r a t e d f o l l o v i n g t h e 7 2 hour d i s s o l v e d oxygen measurements. n e a s u r e m e n t ~ ~ n a daet 7 2 hours before a e r a t i o n are noted above i n t h e column headed -bm and t h e mealrurolments made a f t e r a e r a t i o n are noted i n t h e column headed 'am.
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T.R. Wilbury Study Number 888-TH
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Tab10 A.1. contlnuod.
Nominal concontr~tion
of N2803-2
(WL)
e8
0 24 48 72 96 Rop.hrhrhrhrht
-Teolp4raturo ('c)
0 24 48 72 96 hthrhrhrhr
0 (control) 1 2
160
1
2
250
1
2
400
1
2
630
1
2
1,000
1
2
7.5 7.4 7.6 7.4 7.6 7.5 7.4 7.6 7.4 7.7
7.5 7.4 7.6 7.4 7.7 7.5 7.5 7.6 7.5 7.7
7.5 7.5 7.6 7.5 7.8 7.5 7.5 7.6 7.5 7.7
7.5 7.5 7.6 7.5 7.7 7.5 7.4 7.6 7.5 7.8
7.5 7.5 7.6 7.5 7.7 7.5 7.5 7.6 7.5 7.8
7.5 7.5 7.6 7.5 7.7 7.5 7.5 7.6 7.5 7.7
21.9 21.9 21.8 21.6 21.6 21.9 21.9 21.9 21.6 21.9
21.9 21.9 21.8 21.6 21.9 21.9 21.9 21.8 21.7 21.8
21.9 21.9 21.8 21.7 21.8 21.9 21.9 21.8 21.7 21.7
21.9 21.9 21.8 21.6 21.9 21.9 21.8 21.8 21.7 21.9
22.0 21.9 21.8 21.7 21.9 21.9 21.9 21.8 21.6 21.9
21.8 21.8 21.8 21.6 21.6 21.9 21.9 21.8 21.6 21.8
T.R. wilbury study Number 888-TH
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04/01 /2001
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ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
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LAB REQUEST NO. N2803 REQUESTOR NAME: SAB/GOEBEL/PICKETT DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 08/01/1995 DESC: FC-143 AND RELATED PRODUCTS ECOTOX WORK
CONTRACT LAB(S): TRWIL EXP COMP DATE: 10/04/1995 DATE COMPLETED: 03/27/1996 PROJECT LEAD: PHONE NO: 3M FAX NO: 651-778-6176
Samples received from S.J. M a r t i n .
Samples 1 - 5 sent t o TRWIL on 8/11/95. 8/22/95.
An a d d i t i o n a l 4L o f sample 5 sent on
Sample 1 = T e t r a b u t y l ammonium hydroxide, 40% i n water. Sample 2 = L-13492 (45% s o l i d s , 27% water, 27% IPA) Sample 3 = FC-26, 100% s o l i d s . Dissolve i n 50:50 water:IPA s o l u t i o n . Sample 4 = FC-143, 100% s o l i d s . Sample 5 = L-13856, 15% s o l i d s . Sample 6 = L-13856, d i l u t e d by a f a c t o r o f 15, t e s t e d as an e f f l u e n t .
Sample #1 (Tetrabutylammonium hydroxide) - a new b o t t l e was ordered f r o m
A l d r i c h because TR Wilbury needed more sample. This was l o t 05922BN, r e d l a b e l , cat. # 17,878,O. sab 9/11/95
NOTE: A l l data f o r sample 3 (FC-26) i s reported i n terms o f FC-26, and n o t as a 50/50 mixture w i t h isopropanol. sab 11/21/95
NOTE: For sample #5, o n l y a r a n g e - f i n d i n g t e s t was run f o r t o x i c i t y t o algae. The requestor canceled t h e request f o r t e s t i n g so no d e f i n i t i v e t e s t was run. The r e s u l t s f o r t h e range f i n d e r are as f o l l o w s :
Test Conc. mg/l 0.1
1.o
10
100 1000 EC50 (95% C.I.) sab 2/16/96
Percent
Difference
i n Avg. S p e c i f i c Growth Rate
96-hrs Expsure
2
18
4
32
- 100
160 (100
1000) mg/L.
For N2803-6, t h e t e s t i n g was canceled before a d e f i n i t i v e study w i t h f i s h could be completed. Results r e f l e c t t e s t i n g w i t h a s i n g l e concentration o f 13% v / v , which showed no m o r t a l i t y a f t e r 96-hours. sab 3/14/96
1 08/11/95 TB NH40H
L o t 10317KQ
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
27 MG/L
(23 - 32)
04/01 /2001
* = CONTRACT LAB DATA
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
BACK TO MAIN
2
1 0 8 / 1 1 / 9 5 TB NH4OH
L o t 10317KQ
- TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR
TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0
7 2 0 MG/L 3.7 MG/L
(600 - 1000) (1.8 - 7.7)
2
L-13492
Lot 2
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0
3
FC- 26
L o t 135
62 MG/L 960 MG/L 8.4 MG/L
( 36- 100)
(830->1000)
(5.9 - 14)
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0
3 6 0 MG/L 1 4 0 MG/L 9 0 MG/L
( 3 0 0 - 500)
(110 - 180) (63 - 130)
4
FC-143
Lot 427
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
- TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR
TRWIL IC5O-ALGAE *ALGAE ASSAY I C 5 0
5 0 8 / 1 6 / 9 5 L-13856
As - is
720 MG/L 740 MG/L > l o 0 0 MG/L
(600 - 1000) (660 - 830)
(-1
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0
190 MG/L
(130-600)
>lo00 MG/L
(-1
SEE COMMENT ABOVE
6
L- 13856
1:15 d i l u t e d
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR
TRWIL ICSO-ALGAE *ALGAE ASSAY - I C 5 0
1.2 % v/v
(1.0
>13 % V / V
(-1
TESTING CANCELED
- 1.7)
* = CONTRACT LAB DATA