Document EvNmpxk06avJ2gZB6Ndgjrwr4
MICROBICS MICROTOXID TOXICITY TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FC-201 F-X. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M producton lotnumber was 1.The testsample isFC201 F-X (F-I1129). Currentinformatioinndicatesitisa mixture3% PFOS, 36% diethyleneglycolbutylether,36.6% water,8.25% acrylicfbamer,7% sodium octylsulfate6,% sulfonatedalkylethersalts,3% triethanolaminea,nd 0.15% toluoltdazole.
The followingsummary applies toa mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotoxC Procedure Test type: Acute static GLP: No Year Completed: 1992 Species: Photobacteriumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearregression program providedforMicrotox& Test organism source: MicrobicsR'eagent. Test conditions:
Diluent:MilliporMeilli-Qwmater Test temperature: Not noted. Stock and testsolutionpreparation:A primarystocksolutiownas prepared by adding 200 mg oftestmaterialto 100 mL MilliporMeilli-Q water. The pH ofthe stocksolutionwas 7.4. An osmoticadjustmentwas made using 200 mg NaCi dissolvedin10 mL stocksolution.Appearance of testsolutionswas noted as "clearand coloriese.Alltestsolutionswere made by proportionadlilutions. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blank control Element basis: Percentlightloss
RESULTS
Nominal concentrations: Blank control,125,250, 500,and 1000 mg/L
Element value:
5-minuteEC50 = 153 (130 - 180) mg/L 15-minuteEC5o = 135 (108 - 170) mg/L 30-minuteECro = 110 (84- 145) mg/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 30-minute EC50 forPhotobacterium phosphoreum was determined tobe 110 mg/L witha 95% confidenceintervaolf84 to 145 mg/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:Jimischranking2. Testingmeets allcriterifaorqualitytesting. However, sample pudty was not propedy characterizedand the studylacks analyticaclonfirmationofthe amount offluorochemicalproportioninthe solution.
REFERENCES
Thisstudy was conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Kl 126-1,1992.
OTHER
Last changed: 6/26/00.
rnIowa -6.0-PWO
imple Description T-C
9DF,Qvrj LIGAlt-D
Environmental Lab - Work Sheet Ler
Lab Request No.
Page
of
Date:
Analyst:
46
-7.-g
fnlal
L-JA'tNif 'V
^i.
C@
Li (;%A ir)
FINAL RESULTS
lllilliltil
NUMBER ------
CONC. ---------
rnin K1126-I.R:5
/-!I/..IMIkill@illlmtiiltIi.iIikl1ll.Ill1IllPlIpIl1.1/1I.i1l1i.l1t1.1
GAMMA
GAMMA EST.
% EFFECT
--------- ----------- ----------
1
125.0000
0.77oO
0.8583
43.5o3
2
125.0()()() 0.79()6
0.8583
44.152
3
250.0000
1.6893
1.5336
62.el6
4
250.0000
1.7168
1.5336
63.191
5
500.0000
3.00:7,6
2.74(-)2
75.023
6
5()()0.00(:,
2.9126
2.74()@::.
74.442
7
looo.00oo
4.6lE3/@
4.8964
82.2o2
a
1o(-)Oo.ol-)(') 4.:7,718
4.8964
81.:7.84
EC 54) 152.6346 ma/L (95% CONIFIDENCE RANGE: 129.7'@*2'3T7O 179.592(-))
lit/i11.i1i1i1i1i.-1i
1-1pliPlill-lill.l1
i;
L-r/-CT-DL(ScFh'ACE BAR to continue.... CCEEL'CCCCECEL'CrrCCEC?-'CCCC'-CCCCCrcCcrCECCCEr'
MICROTOX DATA REPORT
FILE NAME: K1126-i.K5 Sam.ole Description:
TEST DATE: TEST TIME:
FC
2C)lF-X Fllle@5- LOT I BROWN LIQUID TEST DATE 4/117/9"@@'
F'rocedureg BASIC In -i4-@-ial Coticeni--.t--A.tion Tes-@--Time: li minutes
ma/L
Osfnoti-r-A-djustment: NaCl Dilk-tt4@onFactor Concentration Uni-ts: i-ngi/L
NUMBER -----
1
CONTROL
4 5 6
e
IT/IO
Io/IT -------
86/ 4"e' 8 /-!r@ 42 84/ 27 E38/ 28 88/1 19 86/ 19 91/ 14 e7/ 14
CONC. ----------
GAMMA ---------
125.OoOo
o.7700(-)#
125.(-)4)0() o. 71?4)59#
250.0000
1.68931#
@450.0()Oo
1.71675#
500. (.-)()()o 3.Oo367-.#
5oo.C)C)O(-)
2.91264#
100c).0000
4.61873#
1
()()()( 4.3)7176#
GO/ 94
BLANK RATIO = 0.8644
lo ZDDDDDDDDDDDDDDDDDDDDDDDDDODDDDDDDDDDDDDDDODDDDODDDDDDDDDDDD?
3SLOPE
.93-f74
.3
A3 m .3 "I
6 4
'.-zzzzzzzzzzzzzzzzzzzzzz
3
.3
2
3
.3 .3
.3
.3
.3
EC 5o
3
.1(-)GDVDDDDDODDDDVDVDDDD,'-VDD.DDDDDDDODDODDDD'-DDDDDDDDDDVDDDDDDODY
I()
1()0 CONCENTRATION
3 3 .3 .3 .3
.3 3
1()E4
EC 50 = 152.6346 (95'/'C.ONFIDENCE RANGE: 1'49.7237 TO 179.592C)) Used +or calculations
STATISTICAL REPORT FOR DATA FILE i@*'.1126-I.K5
ESTIMATING EQUATION: LOG C = 1.1702 .@LOG b +2.187.7
NUMBER OF DATA PAIRS USED: a
RESIDUAL VARIANCE:
95% CONFIDENCE FACTOR: 1.17661 FOR EC54-)
CORRELATION
COEFFICIENT:
R = 0.98991
EFFECTIVE CONCENTRATIONS
(95% CONFIDENCE RANGE):
EC 50 = 152.6346 EC 20 = 30.1391 EC 10 = 11.6684 EC 1 = 0.7053
ma/L mg/L mq/L ma/L
( 129.7237 TO ( 20.8449 TO
7.o78l TO ().288o TO
179.5920) 43.5773)*** 19..-:L355)*** 1.7c@Z.72)***
EXTRAPOLATED
RESULTS
FINAL
NUMBER ------
I 2 3 4 5 6 7 a
RESULTS
1.11.11-f
1./1Iill-IM1I1-iii-fIilli-1i-i1-1i-i1tM1ii,l-1@1,1
CONC.
GAMMA
GAMMA EST.
% EFFECT
--------- --------- ----------- ----------
125.OOoC)
l@:.5.00o(-)
250.()()00
250.()()()0
500.000o
5oo.000o
1
C)00()
o.e951 o .,gc@L12 1.9307 1.9475 3.3345 3.50(:)e 5.:1,499 4.6():7.8
(:).96()4 ().96()4 1 . 7@4 1 x@;. 1.7212 7-,.OB47 3.0847 5. 5.-;L1B 5 .5 x@8L 1
47.e@:.31 45. t)9"@ 65. E379 66.07:7. 76.929 77.782 64.252 62.155
EC 5() 135.4463 ma/L (95% CONFIDENCE RANGE- 108.2()1':,TO 169.5515)
I-i1i-.i/it-.!1/-Illlliililii1i-IiiI/iI'I1I.I11-IMI-I
.rECL'Tauch
OJPACE BAR to conti-nue
/'I/I,Ilil1y.i1-1I.I1I/1I1mf111.1
1.11.11,IM
.... ECCEEL'CCECEECCCCEECCL'CCECCCCEECCEECCCCECCEE@
1.11m11I'll'!
cr-
MICROTOX DATA REPORT
FILE NAME: K1126-l.Kil'i Sample Description:
TEST DATE: TEST TIME:
FC
2(DIF-X F11129 LOT 1 BROWN LIQUID TEST DATE 4/17/92
Procedure: BASIC Initial Concentration Test Time: 15 minutes
; 10()0 mg/L
Osmotic Adjustment: NaCl Dilution Factor : 2 Concentration Units: mc3lL
NUMBER -----
I
2 3 4 5 6 7 a
IO/IT -------
e6/ 87/ 84/ .98/ BB/ 86/ 91/ 97/
38 4() @::.4 @:*5 17 16 12 13
CONC. ----------
GAMMA ---------
125.000o 125.00()c--)
250.(--)()0() 250.0(--)(--)0 5oo.oooO 50c).(7)ooO lot-)().OOCJO lo()O.oooo
o.895o6# o.82124# 1.9:7o73# 1.94748# 3.33453# @;,.5oo77# 5.34992# 4.60382#
CONTROL IT/10
77/ 94
BLANR: RATIO = (-)..9374
IC) ZDDDDDDDDDDDDDDDDDDDDDDDDDDODDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD?
3SL.OPE
o.8417
7
r_ m m A
I
.1c:)
.3
6
.3 .3
4
.3 >zzzzzzzzzzzzzzzzzzzzz-.-
3
2
.3
.3
.3
.3 .3
3
.3
EC 50
.3
t3DDDDDDDDDDDDDDDDDDO"-DV.DDDDODDDDDDDDDDD@*-DDDDDDDDDDDDDDDDDDD)-'
lo
100 CONCENTRATION
10(')0
.3 .3 3 3 3 3 .3 .3 .3
3 .3 3 3 3 .3 3 .3 .3
IOE4
EC 5o = 135.446.---7(95% CONFIDENCE RANGE: 108.2013 TO 169.5515) Used for calculations
cr-
STATISTICAL REPORT FOI;!DATA FILE i."1126-I.KI5
ESTIMATING EQUATION: LOG C = 1.1494 x LOG b +,:,,.i3ie
NUMBER OF DATA PAIRS USED: 8
RESIDUAL VARIANCE:
0.00492
95% CONFIDENCE FACTOR:
1.25le(D FOR EC5(:)
CORRELATION
COEFFICIENT.- R
o.9E3:7,"JE3
EFFECTIVE CONCENTRATIONS
(95% CONFIDENCE RANGE): -
EG 5(D = 135.4463 EC 2(:)= 27.5266 EC 10 = 10.8380 EC I = 0.6886
ma/L mg/L mg/L ma/L
( 108.2013 TO ( 16.8924 TO
5.6365 TO 0.2175 TO
169.5515) 44.8552)*** 20.8398)*** 2.17?8)***
EXTRAPOLATED
RESULTS
FINAL mmi-immmilml mmmm
NUMBER ------
I 2 3 4 5 6 7 a
RESULTS
rnIt)
K 1126- 1. 1'%'30
CONC.
GAMMA
--------- ---------
125.OOoO 125.OOoC) 250.0000 250.000t) 50o.0000 50().04)()C) 100(@).0000 100().0000
1.0258 0.997..9 2.:39:'0 2.3920 3.9749 4.2o9l 6.0152 5. 7()6E3
GAMMA EST. -----------
1.1536 1.1536 2.0627 -,:..o627
3.6883 3.6E3.33 6.5950 6.595c)
EFFECT ----------
50.636 49.84e 70.519 7().519 79.E399 $3O.8o3 85.745 E35. 090
EC 50
110.3339 ma/L (95% CONFIDENCE RANGE: 83.8()96 TO 145.2529)
111.111.-1imi-it-I
1.11.IPImM 1.1III-ii-filli-IMMI.IMMt1i.l1lfill-Illilm/i.f1ilmm 1.11-1111mili!.i
L'EEL'Touch
SPACE BAR to continue....
/./IIIil.Ilitilimmi.1 1-tilimilill
MICROTOX DATA REPORT
FILE NAME: K1126-l.K30 Sample Description;
TEST DATE: TEST TIME:
FC
2()IF-X F11129 LOT I BROWN LIQUID TEST DATE 4/17/92
Procedure: BASIC Initial Concentration Test Time: 30 minutes
: 1000 ma/L
Osmotic Adjustmet-it: NaCl Dilution Factor : 2 Concentration Units: ma/L
NUMBER -----
1
:3 4 5 6 7 a
IO/IT -------
86/ 36 87/ ::@7 84/ 21 E38/ 22 BB/ 15 e6/ 14 91/ 11 87/ 11
CONC. ----------
125.0(joo 250.000() @e_5o.0000 500.0000 50o.0000 1000.0000 1000.4)00()
GAMMA ---------
1.02576# ().99392# 2.3?196# 2.39196# 3.974aB# 4.20908# 6.01519# 5.70683#
CONTROL IT/IO
79/ 94
BLANK RATIO = 0.848()
ZVDDDDDDDDDDDDDVDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD?
3SLOF'E = 0.8384
.3
.3
.3
6
A .3
5
m .3
m .3
4
A .3
.3
1 >zzzzzzzzzzzzzzzzzzz-
1
3
2
.3
.3
.3
.3
i
EC 5()
.3
.10 @DDDDDDODDOODDODDDDD-'N.DDDDDDDDDDDDDDDDDD@*-DDDDDDDDDDDDDDDDDDDY
10
10o CONCENTRATION
lOoO
3
.3 3
.3 .3 3 < .3 .3 3 .3 3 .3 3 .3 .3
lOE4
EC 50 = llo.3339 (95% CONFIDENCE RANGE: 83.8096 TO 145.2529) Used for calculations
STATISTICAL REPORT FOR DATA FILE K1126-l.K:Zo
ESTIMATING EQUATION:
LOG C = 1.1478 4 LOG b +2.C)427
NUMBER OF DATA PAIRS USED: 8
RESIDUAL VARIANCE:
0.00569
95'/'CONFIDENCE FACTOR: CORRELATION COEFFICIENT:
1.31648 FOR EC51)
R = o.9eO97
EFFECTIVE CONCENTRATIONS
(95% CONFIDENCE RAN8E):
EC-50 = EC 20 = EC 10 = EC 1 =
110.3339 22.4741 8.8605 0.5652
ma/L mg/L ma/L mall
83.6096 TO 12.7709 TO
4.2097 TO o.1568 TO
145.2529)*** 39.5497)*** 18.6493)*** 2.0365)***
EXTRAPOLATED
RESULTS