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BACK TO MAIN BACK TO MAIN I. GOOD LABORATORI PRACTICE COMPLIANCE STATEMENT Thi study was conductad according to EPA Good Laboratory Practice Regulations (40 CFR 792), with the following exceptions. The stability of the test substance under test conditions was assumed but not verified at T.R. Wilbury Laboratories. Deviations from the study protocol are presented in section XII of this report. Petpr L. Kowalski Study Director T.R. wilbury study Number 896-t h Page 2 of 23 BACK TO MAIN II. QUALITY ASSURANCE STATEMENT Submitted byt T.R. Wilbury Laboratories, Inc. 40 Doaks Lane Marblehead, Massachusetts Certification! T.R. Wilbury Laboratories, Inc. study number 896-TH, Growth and Reproduction Toxicity Test with N2803-4 and the Freshwater Alga, Selenaatrum capricocnuturn, was audited by the Quality Assurance Unit for compliance with the protocol, standard operating procedures, and applicable Good Laboratory practices (U.S. EPA, 1993). Quality assurance audits were performed anu the findings reported to T.R. Wilbury Laboratories management and the study director on the following dates: Aspect Audited Audit Date: Reported to Study Director Reported to Management Protocol: In-life: Draft Report: Final Report: 8/ 4/95 9/26/95 1/18/96 3/ 7/96 8/ 4/95 9/26/95 1/18/96 3/ 7/96 8/ 4/95 3/ 7/96 3/ 7/96 3/ 7/96 Arthur P. Paradice Quality Assurance officer T.R. wilbury study Number 896-TH Page 3 of 23 BACK TO MAIN III. TABLX OP COMTEMTS SECTION > I. Good Laboratory Practice Compliance statement 11. Quality Assurance Statement III. Table of Contents IV. Index of Tables and Figures V. summary VI. General Information VII. Introduction Vili. Methods and Materials IX. Results X. References XI. Signature Page XII. Protocol Amendments and Deviations Appendix A. Hater Quality Data from The Toxicity Test PAGE 2 3 4 5 6 7 8 8 13 18 19 20 21 T.R. wilbury study Humber 896-TH Page 4 of 23 IV INDEX or TABUESANDriOUNKS Tabla 1. Chemical Characterization of a representative sample of water used to formulate test media for the toxicity test with the freshwater alga, Selenaatrum caprlcornutum, and N2803-4. Tabla 2. Test system summary for the toxicity test with N2803-4 and the freshwater alga, Selenestrum caprlcornutum. Table 3. cell growth data from the toxicity test with N2803-4 and the freshwater alga, Selentatrum caprlcornutum. Table 4. Average specific growth rate and percent change from the control from the toxicity test with the freshwater alga, Selenaatrum caprlcornutum, and N2803-4. Table 5. Effective concentrations (EClOs, EcSOs, EC90s) from the toxicity test with the freshwater alga, Selenaatrum caprlcornutum, and N2803-4. Table A.l . Temperature measured during the toxicity test with N2803-4 and the freshwater alga, Selenaatrum caprlcornutum. Table A.2 . pH values measured during the toxicity test with N2803-4 and the freshwater alga, Selenaatrum caprlcornutum. Figure 1. Growth of Selenaatrum caprlcornutum exposed to N2803-4 for 96 hours. PAGE 9 11 14 16 17 22 23 15 1:;' T.R. wilbury study Number 896-TH Page 5 of 23 BACK TO MAIN V. SUMMARY The toxicity of N2803-4 to the freshwater alga, Selenastrum caprlcormitum, is described in this final report. The test was conducted for 3M Company for 96 hours from September 22 to 26, 1995, at T.R. Wilbury Laboratories, Inc. in Marblehead, Massachusetts. It was conducted according to the protocol developed for T.R. Wilbury Study Number 896-t h . N2803-4 was supplied by the sponsor. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 24 1 2*C. Nominal concentrations of N2803-4 were 0 (control), 62, 130, 250, 500, and 1,000 mg/L. The dilution water was sterile enriched media adjusted to a pH of 7.5. Exposure of algae to the test substance for 96 hours resulted in a median effective concentration (EC50) of 310 mg/L N2803-4 (95% confidence interval - 280 to 350 mg/L) when calculated using the number of cells/ml, and greater than 1,000 mg/L when calculated using the average specific growth rate. The 96 hour no observed effect concentration (NOEC) is 62 mg/L and the lowest observed effect concentration (LOEC) is 130 mg/L when calculated using the number of cells/ml and the NOEC is 500 mg/L and the LOEC is 1,000 mg/L when calculated using the average specific growth rate. T.R. wilbury Study Number 896-TH Page 6 of 23 BACK TO MAIN VI. ORNERAL INFORMATION Materiel Teetedi Sponsor Test Substance Shipped Promt Reported Purity: Study Initiation Date: Experimental start Date: Experimental Completion Date: Study Completion Date: Archive Location for the Raw Data and a Copy of the Final Report: N2803-4 3M company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 55144 3M Company Environmental Laboratory 935 Bush Avenue Building 2--3E-09 St. Paul, Minnesota 55144 100 August IS, 1995 (protocol signed) September 22, 1995 September 26, 1995 March 7, 1996 T.R. Wilbury Laboratories, Inc. Marblehead, Massachusetts T.R. wilbury study Number 896-TH Page 7 of 23 VX I INTRODUCTION This study was sponsored by 3h Company. Tha objective of the study was to determine the 24, 48, 72, and 96 hour effective concentrations (EClOs, EC50s, EC90s) and no observed effect concentration (NOEC) of tha test substance to the freshwater alga, Selenastrum capricornutum, under static conditions. The test protocol was based on U.S. EPA methods (EPA, 1993). The report contains sections that describe the methods and materials employed in the study, and results of the investigation. The report also contains an appendix that presents water quality data collected during the test. VIII. METHODS AND MATERIALS TEST SUBSTANCEI The sample of N2803-4 (T.R. wilbury Laboratories Sample Number 522) was delivered to T.R. Wilbury Laboratories on August 14, 1995 in a cardboard box at ambient temperature. It was contained in an 50 ml glass jar. The label attached to the bottle included the following information: "N2803-4, (See MSDS)". N2803-4 (white powder) was supplied by 3M Company, St Paul, Minnesota. Prior to use the test substance was stored in the dark at room temperature. The test substance was assumed to have a purity of 100% active ingredient and to be stable under storage and testing conditions. Unused test substance will be returned to the sponsor. DILUTION WATER: Water used for acclimation of test organisms and for all toxicity testing was sterile enriched media (U.S. EPA, 1978; T.R. Wilbury Standard Operating Procedure number 6) adjusted to a target pH of 7.5 with 0.1 N HCl prior to use. characterization of a representative sample of water used to formulate media is presented in Table 1. TEST ORGANISM: Algae used for the test (SeienaBtrwa capricorrmtum, UTEX 1648) was from a culture originally procured from the culture collection of Algae at the University of Texas at Austin and delivered to T.R. Wilbury Laboratories on April 11, 1995. The culture was transferred to sterile enriched media identical to media used for this test and maintained at test conditions for at least 14 days before the definitive test. The subsample of algae used to inoculate media at the start of the definitive test came from an 8 day old culture. Identification of the culture organisms was verified using an appropriate taxonosiic key. T.R. Wilbury Study Number 896-TH Page 8 of 23 BACK TO MAIN chemical characterization of a representative sample of water used to formulate test media for the toxicity test with the freshwater alga, Salanaatrum capricornutua, and N2803-4. Parameter1 Unit of Measurement Detection bimit Measured Value Metals Aluminum Arsenic Boron Cadmium Chromium Cobalt Copper Iron bead Mercury Nickel Silver Zinc Nitrate Chloride Fluoride Total Organic carbon Total Phosphorus Organochlorine Pesticides Toxaphene Organophosphorus Pesticides Dimethoate TEPP Monocrotophos PCBs " mg.'b mg/b mg/b mg/b mg/b mg/b mg/b mg/b mg/b mg/b mg/b mg/b mg/b mg/b as N mg/b mg/b mg/b mg/b pg/b pg/b pg/b pg/b pg/b pg/b pg/b 0.1 0.01 0.5 0.0002 0.01 0.03 0.005 0.03 0.005 0.0003 0.03 0.02 0.02 0.05 1 0.1 1 0.03 0.5 2 0.5 2.0 2.0 2.0 0.5 NDJ ND ND ND ND ND ND 0.03 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND Notesi ,v; ' ,' : 1. Parameters were measured in deionized water (used to formulate dilution water) that was collected during August, 1995 and analyzed by Face, Inc. as part of routine water quality testing. ' .... ..1, . 2. ND not detected at or above the detection limit. > T.R. Wilbury study Number 896-TH Page 9 of 23 BACK TO MAIN Results of a range finding t;aat wara unad to aalact tha concantrationa for tha dafinitiva test. Tha ranga finding taat was p a r f o m a d from saptembar 7 to 11, 1995. At tha conclusion of tha taat tha nunbar of calls/nl aquallad tha following parcant of tha control* 0.10, 1.0, 10, and 100 mg/L - >100 and 1,000 mg/L - 0. Tha definitive toxicity test was performed from September 22 to 26, 1995, according to T.R. wilbury Protocol 896-TH (Growth and Reproduction Toxicity Test with N2803-4 and the Freshwater Alga, Salanaatcum caprlcornutum). The protocol, which wan signed by tha study director on August 16, 1995, is based on procedures of the U.s. Environmental Protection Agency (1993). A summary of the test system is presented in Table 2. Tha test was conducted at a target temperature of 24 t 2*C with five concentrations of test substance and a dilution water control. Nominal concentrations of the test substance were: 0 mg/L (control), 62, 130, 250, 500, and 1,000 mg/L. A 1,000 mg/L stock solution was formulated on September 22, 1995 by adding 0.5 g N2803-4 to sterile* enriched media and adjusting the final media volume to 500 ml. Appropriate amounts of this stock solution were added directly to dilution water to formulate test media. Algae were distributed among three replicates of each treatment at the rate of approximately 10,000 cells/ml. The test was performed in 250 ml glass Erlenmeyer flasks that contained 50 ml. of test solution. Test vessels were randomly arranged on a rotary shaker adjusted to 100 rpm in an incubator during the test (a random numbers table was used to select the location of each vessel). A 24 hour light and 0 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of approximately 400 footcandles. The number of algal cells/ml in each test vessel and the occurrence of relative size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers, or aggregation of cellB was determined visually by means of direct microscopic examination with a hemocytometer. Cell counts were made and recorded daily during the 96 hour test. The determination of whether toxic effects were algistatic or algicidal was performed at the conclusion of the toxicity test (0.5 ml of media from each 1,000 mg/L vessel was transferred to a vessel containing fresh media without N2803-4 and incubated for 72 hours under test conditions). T.R. Wilbury Study Number 896-TH Page 10 of 23 BACK TO MAIN Table 2. Teat ayatera summary lor the toxicity teat with N2803-4 and tha freshwater alga, Salanaatrum caprlcornuturn. Speciesi Selenaatrum capricornuturn Culture Acclimation Periodi >14 days Test Duration! 96 hours Test Vessels: 259 ml glass flasks Volume o TeBt Media: 50 ml Number of Replicate Test Vessels: 3 Inoculum: -19,000 cells per ml Oscillation Rate: 100 rpm Incubator Temperature Range: 23.5-23.7`C Photoperiod: 24 hours light and 0 hours dark. Light Intensity: -400 footcandles Hater Quality Measurements: pH in each test vessel at the beginning and end of the test. Incubator temperature daily. Dilution water analysis is summarized in Table 1. End Points: 24, 48, 72, and 96 hour EC10, EC50, and EC90; no observed effect concentration. Acceptability criteria: Logarithmic growth at 96 hours and acceptable temperature range. 1 1 1 1 I I 1 1 I 1 1 1 1 1 1 1 1 1 1 1 1 T.R. Wilbury study Number 896-TH Page 11 of 23 I BACK TO MAIN Temperature of tha Incubator was m a t u r e d and recorded daily (thermometer number 2968), and pH (Beckman model pdl 12 meter; instrument number 124) vaa determined in each tent venial at the beginning and end of the teat. The temperature in a representative vessel of water incubated with the teat veaaela waa continuously recorded. STATISTICAL METHODSI The average specific growth rate was calculated as the natural log of the number of cella/ml at 24, 48, 72, and 96 hours minus the natural log of the number of cella/ml at 0 hours divided by the exposure period. The percent change from the control was calculated by subtracting the treatment average specific growth rate from the control average specific growth rate, dividing the difference by the average specific growth rate in the control, and multiplying that value by 100. Results of the toxicity test were interpreted by standard statistical techniques. The binomial/interpolation or probit methods (Stephan, 1983) were used for calculating EC10, EC50, and EC90 values. All calculations were performed using the number of cells/ml and the average specific growth rates and nominal concentrations of N2803-4. The no observed effect concentration (NOEC) was determined using a parametric one-way analysis of variance (ANOVA) or a non-parametric Kruskal and Wallis' test and the number of cells/ml and the average specific growth rate in each test vessel at the end of the test. T.R. Wilbury study Humber 896-TH Page 12 of 23 XX. RESULTS No insoluble material was noted in an/ teat veaael daring the teat. The algal population in the control vessels grew well, reaulting in an average of 1,407,000 cella/ml after 96 hours (Table 3). Water quality throughout the test waa within acceptable limits (Appendix A). The range of incubator temperatures was 23.5 to 23.7*C (Table A.l). The pa of test media (Table A.2) was slightly affected by the test substance only at 1.000 mg/L at the beginning of the test. Biological data generated by the acute toxicity test with N2803-4 are presented in Tables 3 and 4. The growth curve for algae exposed to the test substance for 96 hours is presented in Figure 1 (the slope of the dose-response curve ia 2.5 based on cells/ml at 96 hours). The 72 and 96 hour EC50 values for algae exposed to N2803-4 are presented in Table 5. The 96 hour EC50 (and associated 95 percent confidence limits) is 310 mg/L (280 and 350 mg/L) when calculated using the number of cells per ml, and greater than 1,000 mg/L when calculated using the average specific growth rate. No effects (size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers, or aggregation of cells) were noted. The 96 hour no observed effect concentration (NOEC) is 62 mg/L and the lowest observed effect concentration (LOEC) is 130 mg/L N2803-4, when calculated using the number cella/ml, and the NOEC is 500 mg/L and the LOEC is 1,000 mg/L, when calculated using the average specific growth rate. The determination of whether toxic effects were algistatic or algicidal was performed at the conclusion of the toxicity test. A 0.5 ml aliquot of media from each 1,000 mg/L vessel was transferred to a vessel containing fresh media without N2803-4 and incubated for 72 hours under test conditions. Algae increased from <10,000 cells/mL to 642.000 cells/mL, indicating that the effect of N2803-4 at this concentration was algistatic rather than algicidal. T.R. wilbury study Number 896-TH Page 13 of 23 BACK TO MAIN Tabla 3. Call growth data from tha acuta toxicity taat with N2803-4 and tha fraahwatar alga, Selanaat.rua caprlcornutum. Nominal Concantration of N2803-4 (mg/L) Rep. Number of Calla/ml x 10' (hour) 0 24 48 72 96 0 (control) 62 130 250 500 1,000 1 2 3 mean % control 1 2 3 mean control 1 2 3 mean % control 1 2 3 mean * control 1 2 3 mean % control 1 2 3 mean t control 10 16 10 18 10 12 10 15 ---- 10 17 10 15 10 15 10 16 100 107 10 16 10 19 10 10 10 15 100 100 10 14 10 15 10 11 10 13 100 87 10 11 10 <10 10 13 10 11 100 73 10 <10 10 <10 10 10 10 <10 100 <67 142 466 1,544 154 584 1,320 180 592 1,356 159 S47 1,407 ---- -- 162 602 1,446 184 576 1,272 168 586 1,616 171 588 1,445 108 107 103 160 556 1,080 144 598 1,184 136 584 1,124 147 579 1,129 92 106 80 138 590 762 174 552 752 140 528 744 151 557 753 95 102 54 92 274 354 104 2J4 484 110 300 492 102 286 443 64 52 31 90 106 200 62 134 216 72 124 128 75 121 181 47 22 13 T.R. wilbury Study Hunbar 896-TH Paga 14 of 23 BACK TO MAIN Figure 1. Growth of Seienastrum caprlcoinutum exposecl to N2803-4 for 96 hours. T.R. Wilbury Study Number 896-TH Page 15 of 23 BACK TO MAIN -.Mssi -X'- Table 4. Average pacific growth rata and parcant changa fro tha control from tha toxicity taat with tha fraihwatar alga, Salenaatrum caprlcornutua, and N2803-4. Nominal Concentration of N2803-4 (mg/L) Avaraga Specific Parcant Changa Growth Rata Prom Control ---------------------- -------------------------- 24hr 48hr 72hr 96hr 24hr 48hr 72hr 96hr 0 (control) 62 130 250 500 1,000 0.017 0.058 0.056 0.052 0.020 0.059 0.057 0.052 0.017 0.056 0.056 0.049 0.011 0.057 0.056 0.045 0.004 0.048 0.047 0.039 0.000 0.042 0.035 0.030 -18 -2 -2 0 0306 35 2 0 13 76 17 16 25 100 28 37 42 T.R. Wilbury Study Number 896-TH Page 16 of 23 BACK TO MAIN mL. uj WBWBW8. MMMwaaan m w i^w M P m w aw M M ^^ Tabi* S. Effective concentration! (EClOa, EC50a, EC90a) fron th* toxicity t*at with th* fraahwatar alga, selenaatrum capricornutua, and N2803-4. Tima EC Value (mg/l.) 95 Percent Confidence Limita (mg/L) Calculated Uaing th* Number of Cell/nl 72 houra 96 houra EC10 - 310 EC50 - S20 EC90 - >1,000 EC10 97 EC50 310 EC90 1,000 110 - 440 250 - 1,000 800 - >1,000 77 - 120 280 - 350 830 - >1,000 Calculated Uaing the Average Specific Growth Rate 72 houra 96 houra EC10 - 470 EC50 >1,000 EC90 " >1,000 EC10 220 EC50 - >1,000 EC90 - >1,000 380 - 550 >1 ,000 >1 ,000 160 - 280 950 - >1,000 >1 ,000 w-- T.R. Hilbury Study Number 896-TH BACK TO MAIN X, RXFKRKNCES Bruca, R.D., and Versteeg. 1992. A Statistical Procedura for Modeling continuous Toxicity Data. Environ. Toxicol, and chan. Voi.11. No.10, pp. 1,485-1,494. Stephan, C.E. 1983. Computer Program for Calculation of LC50 Values. Personal communication. U.3. EPA. 1993. 40 CFR Part 797. Toxic Substances Control Act Test Guidelines; Final Rules. Section 797.1050. U.S. EPA. 1993. 40 CFR Part 792. Toxic Substances Control Act (TSCA); Good Laboratory Practice Standards; Final Rule. T.R. Wilbury Study Number 89S-TH' Page 18 of 23 BACK TO MAIN BACK TO MAIN XII. PROTOCOL AMENDMENTS AMD OBVIATIONS PROTOCOL AMENDMENTS! The protocol was amended to specify tbe nominal concentrations for the definitive study. No other amendments were made to the protocol. PROTOCOL DEVIATIONS) Two nominal concentrations were slightly different than the ones listed in the protocol. This deviation did not affect the outcome of this test and no other deviations were made from the protocol. t .r . wilbury study Number 896-TH BACK TO MAIN Appendix Al MATER QUALITY DATA FROM TBE TOXICITT TEST i T.R. wilbury study Number 896-TH Page 21 of 23 BACK TO MAIN Tabi A.l. Temperature maaaurad during th toxicity taat with N2803-4 and tha freshwater alga, Salanaatrua capclcornutum. Hour of Exposure Temperature of Incubator (*C) 0 23.5 24 23.6 48 23.6 72 23.7 96 23.5 tf t* i T.R. Wilbury Study Number 896-TH Page 22 of 23 BACK TO MAIN Tab! A.2. pH valuaa maaaurad during tba toxicity taat with N2803-4 and tha fraahwatar alga, SeJenaatrua caprlcornutua. Nominal Concentration of N2803-4 (mg/L) Replicate pH initial Final 0 (control) 65 130 250 500 1,000 1 2 3 1 2 3 1 2 3 i 2 3 1 2 3 1 2 3 7.5 8.2 7.5 9.6 7.5 9.6 7.5 8.9 7.5 9.0 7.5 8.6 7.5 6.4 7.5 6.5 7.5 6.5 7.5 5.8 7.5 5.4 7.5 5.4 7.5 5.1 7.5 5.0 7.5 5.0 7.4 5.4 7.3 5.4 7.3 5.5 T.R. Wilbury study Humber 896-TH Page 23 of 23 V ~r/ V / fcV V * uii lnv/i'inLirrm - Lnwwnn i v ARCHIVED FINAL REPORT BACK TO MAIN LAB REQUEST NO. N2803 REQUESTOR NAME: SAB/GOEBEL/PICKETT DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 08/01/1995 DESC: FC-143 AND RELATED PRODUCTS ECOTOX WORK CONTRACT LAB(S): TRWIL EXP COMP DATE: 10/04/1995 DATE COMPLETED: 03/27/1996 PROJECT LEAD: PHONE NO: 3M FAX NO: 651-778-6176 Samples received from S.J. Martin. Samples 1-5 sent to TRWIL on 8/11/95. An additional 4L of sample 5 sent on 8/22/95. Sample 1 = Tetrabutyl ammonium hydroxide, 40% in water. Sample 2 = L-13492 (45% solids, 27% water, 27% IPA) Sample 3 = FC-26, 100% solids. Dissolve in 50:50 water:IPA solution. Sample 4 - FC-143, 100% solids. Sample 5 = L-13856, 15% solids. Sample 6 = L-13856, diluted by a factor of 15, tested as an effluent. Sample #1 (Tetrabutylammonium hydroxide) - a new bottle was ordered from Aldrich because TR Wilbury needed more sample. This was lot 05922BN, red label, cat. # 17,878,0. sab 9/11/95 NOTE: All data for sample 3 (FC-26) is reported in terms of FC-26, and not as a 50/50 mixture with isopropanol. sab 11/21/95 NOTE: For sample #5, only a range-finding test was run for toxicity to algae. The requestor canceled the request for testing so no definitive test was run. The results for the range finder are as follows: Test Cone. Percent Difference in Avg. Specific Growth Rate mg/1 96-hrs Expsure 0.1 2 1.0 18 10 4 100 1000 32 100 EC50 (95% C . I .) 160 (100 - 1000) mg/L. sab 2/16/96 For N2803-6, the testing was canceled before a definitive study with fish could be completed. Results reflect testing with a single concentration of 13% v/v, which showed no mortality after 96-hours, sab 3/14/96 ------------ -------- -------- ----------- -------------------- ----- -------- - - -------- - 7 ---------- ----- ---- -- - SAMPLE DATE CODE DESCRIPTION RESULT MDL or (95% C.I.) 1 08/11/95 TB NH40H Lot 10317KQ TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST 27 MG/L (23 - 32) u v i i I n n v I l h DTTTrr 04/01/2001 LAB REQUEST NO. N2803 SAMPLE DATE CODE ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT CONTINUED DESCRIPTION RESULT BACK TO MAIN 2 MDL or (95% C.I.) 1 08/11/95 TB NH40H Lot 10317KQ TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR 720 MG/L TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 3.7 MG/L (600 - 1000) (1.8 - 7.7) 2 L-13492 Lot 2 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 62 MG/L 960 MG/L 8.4 MG/L (36-100) (830->1000) (5.9 - 14) 3 FC-26 Lot 135 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 360 MG/L 140 MG/L 90 MG/L (300 - 500) (110 - 180) (63 - 130) 4 FC-143 Lot 427 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 5 08/16/95 L-13856 As-is 720 MG/L 740 MG/L >1000 MG/L (600 - 1000) (660 - 830) (-) TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 6 L -13856 1:15 diluted 190 MG/L (130-600) >1000 MG/L (-) SEE COMMENT ABOVE TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 1.2 % V/V (1.0 - 1.7) >13 % V/V (-) TESTING CANCELED * = CONTRACT LAB DATA