Document Eqke9Qx4naVG60xqJ0LYJyDQj

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:N -EthyplerfluorooctanesIufonamide; may alsobe referredto as U 1464, ETFOSA, ETPOSA, F-6309, or FX-1 2. (1Octanesulfonamide,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-N-ethylC-A,S # 4151-50-2) Remarks: Materialisa whitesolid.Sample purityand lotnumber were not recorded. METHOD Method: Adapted from OECD 203 - Water accommodated fractions Type: Staticacute GLP: No Year completed: 1998 Species: Pimphales promelas Supplier:ASci in-housecultureD,uluth,MN Analyticalmonitoring: Nominal concentrationosnly. Temperature,pH and dissolvedoxygen recorded. Exposure period:96-hours Statisticamlethods: LL50(lethalloading)values were calculatedusing the probitmethod. The NOEL was calculatedby FishersExact Test, TOXSTAT SoftwarePackage, Version3.5,Laramie,Wyoming. Test fishage: < 24-hour old Length and weight:: Not measured Loading: Not measured Pretreatment: None Test conditions: Dilutionwater: Carbon-treateddechlorinatedCityofDuluth,MN tap water aeratedfor24-hours priorto use inthetest. Dilutionwater chemistry during the study: Hardness: 40-42 mg/L as CaC03 DO: 8.1-8.9mg/L Alkalinity 40-42 mg/L as CaC03 pH: 7.8-8.1 Conductivity 113-122 pmhos/cm Test temp. range: 21.1-21.40C Stock and test solution preparation:Test solutionsprepared by mass additionoftestsubstance to 1.5Laliquotsofdilutiownater (one per concentration)T.he solutionwsere vigorouslystirred (vorte1x/3to'/2solutdieopnth)for20 hours.The aqueousphase was siphonefdromthevesselatmid-depth. ConcentrationdsosingrateO:nce StabiliotfythetestchemicalsolutionsN:otrecorded Exposurevessels:250 mL borosilicgaltaessbeakers Exposurevolume:200 mL Number ofreplicatetsw:o Number offishperreplicattee:n Number ofconcentrationFsi:veplusa negativceontrol RESULTS NominalloadingconcentrationBsk:contro7l8,,130,216,360,600 mg/L Elementvalue: 24-hourLL50:308 (238-380m)g/L 48-hourLL50:216 (168-264m)g/L 72-hourLL50:216 (168-264m)g/L 96-hourLL50:206 (162-247m)g/L 96-hourLL,o:115(66-150m)g/L 96-hourNOEL: 130mg/L PercentMortality NominalLoading Concentrabon, 24-Hours mg/L Negabve Control 5 78 5 130 5 216 35 360 60 600 90 48-Hours 5 5 10 70 80 95 72-Hours 5 5 10 70 80 95 96-Hours 5 5 10 75 80 100 Remarks: Allvalues reported are fornominal loadingconcentrations. No measurement of testconcentrationswas conducted. CONCLUSIONS The Et FOSA 96-hour LL50forfathead minnow was determined to be 206 mg/L with a 95% confidence intervalof 162-247 mg/L Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY ReliabiliKtlyi:miscrhankin2g.Thisstudyw,hilewellconductedusingthe methodology acceptableatthe time,lacksanalyticadlata for determinationofthetestsubstance concentrationinthe testsolutionsand determinationofthe sample purity.Testingwas alsodone wellabove the solubililtiymiotfthetestsubstancewiththe use of water accommodated fractions. REFERENCES Study conducted atthe requestof3M Company by,ASci Corporation, Duluth,MN. OTHER Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 Last changed: 5/18/00 AScl CorporaLion/AS-cDiuluth EnvironmentaTlestingDivision Submittedto3M Co. - 5198 AScl StudyID# 5010-068 STUDY TITLE ACUTE TOXICITY OF U1464 TO LARVAL F,ATHEAD NffNNOW (Pimephalespromelas) DATASTANDARD ADAPTED OECD GUIDELINE 203 STUDY COMPLETED May 4, 1998 TESTING FACILITY AScl Corporation/AScI-Duluth Environmental TestingDivision 4444 Airpark Boulevard Duluth,MN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATION NUMBERS ASCI Study ID# 5010-068 3M Company Lab Request# U1464 Sponsor: 3M Company Sponsor Study ID# U1464 or ASel Corporation/AScl-Duluth Environmental Testing Division Submitted to 3M Co@ - 5/98 AScl Study ID# 5010-068 1.0 GENERAL INFORMATION StudyTitle Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# TestingFacility PrincipalInvestigator ProjectManager TestingFacilityDirector DefinitiveTest Dates Data Validationand Report Review Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport Acute Toxicityof U1464 to LarvalFathead Minnow (Pimephalespromelas). Adapted OECD Guideline203. 3M EnvironmentalTechnology and Safety Services,Building2-3E-09, 935 Bush Avenue, St.-Paul,MN 55133; Tel No. (612) 778-74520 RochelleR. Robideau, 3M Environmental Technology and SafetyServices,Building23E-09, 935 Bush Avenue, St.Paul, MN 55133; Tel No. (612)778-7065. U 1464. AScl Corporation/AScl-DulutEhnvironmental TestingDivision,4444 A7irparkBoulevard, Duluth,MN 55811;Tel. No. (218)722-4040. Todd McGuire Joe Amato Donald Mount April 15-19,1998. Alan Mozol Report Si9natur A @@7 Two yearsfrom the date study iscompleted. ASCI Corporation/AScl-DuluthEnvironmental TestingDivision,4444 Airpa-rkBoulevard, Duluth,MN 55811; Tel.No. (218)722-4040. Sponsor: 3M Company Sponsor Study ID# U1464 2 2.0 OBJECTIVE(S) AScl Corporation/ASci-Duluth EnvironmentaTlestingDivision Submittedto3M Co.- 5/98 AScl StudyID# 5010-068 To determinethe96-hour10% and 50% lethalloading(sLLIOand LL50),and the96-hourno observableeffectloading(NOEL) of the testsubstance,U1464, for larvalfatheadminnow (Pimephalespromelas) under statitcestconditions. This study was conducted accordingto the AScl studyplan retainedby the Sponsor and OECD Guideline203 (OECD 1993). The testsolutionswere water-accommodated fraction(sWAF) preparedfollowingGirling,Whale, & Adema 1994. 3.0 TEST METHODS Test Substance Properties:U1464, whitecrystalss,pecificgravity= 1.7 (water = 1), stable,and negligiblseolubilitiyn water. Test Organisms Testloadings: 0 (dilutiownater control)78 mg/L, 130 mg/L, 216 mg/L, 360 mg/L and 600 mg/L. Testsolutionswere preparedby mass additionof testsubstanceto 1.5 L aliquotsof dilutionwater. The solutionswere vigorouslystirre(dvortex1/3 to 1/2 solutiondepth)for20 h. The aqueous phase was siphoned from the vesselat mid depth. Source: Healthy, 24 hour old fathf-adminnow larvae(Pimephales promelas) obtainedfrom AScl-DETD in-house culture. Holdinglacclimation:The fishused in the testswere obtained from AScl's in-houseculture.The fishwere culturedin water of thesame qualityas the testdiluent. DilutionWater Use in test:Used 10 organisms/replicataend 2 replicates/test concentration. Source: Carbon-treatedechlorinateCdity of Duluth, MN, tapwater. The most recentchemicalanalysisof postcarbon (PC) water isin theRaw Data Package. Exposure Chambers Sponsor: 3M Company Sponsor Study ID# U1464 CharacteKstics: At testtime, the water had a hardness of 40 mg/L (as CaC03) and a pH of 7.87. Aerated for24 h priorto use in thetest. 7@pe: 250-ml borosilicatgelassbeakers. Use in test:200 ml of dilutionwater or testsolution/cahmber. During the test,kept covered, exceptwhen experlmental observationswere made. Solutionswere not aeratedduring the exposures. 3 Incubation Observations Endpoint Calculations ASci Corporation/AScl-Duluth F-nvironmental Testing Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 Duration: 96 hours. Daity photope7iod: 16 h lightand 8 h dark, maintainedusing cool-white fluorescentlamps. Temperature: 21.0' + I*C. Biological: Daily- Test organisms with abnormal behavior and signsof stress,and dead testorganisms. Water chemistry: (1) At testinitiationt-otalhardnessand alkalinity(,2) daily-temperature,DO, conductivitya,nd pH. AU determinationswere made accordingto APHA methods (1995). 24-, 48-, 72-,and 96-h median lethalloading.(LL50s), 96-h lethalloading (LL,,),and 96-h no observableeffectloading (NOEL) LLIO and LL50: EPA Probitmethod. NOEL: Fisher'sExact Test.TOXSTAT Laramie, Wyoming. SoftwarePackage. Version 3.5. Sponsor: 3M Company Sponsor Study ID# U1464 4 ASci Corporation/AScl-Duluth EnvironmentaTlestingDivision Submittedto3M Co. - 5/99 ASci StudyID# 5010-068 3.0RESULTS TestSubstancTeoxicit(y95% Confidence IntervalP)ertinenDtata areinTable 1 24-hLL50: 308 mg/L (238-38m0g/L). 48-hLL50: 216 mg/L (168-264mg/L). 72-hLL50: 216 mg/L (168-264mg/L). 96-hLL50: 206 mg/L (162-247mg/L). 96-hLLIO: 115 mg/L (66-150mg/L). Ranges of Water Chemistry Parameters 96-h NOEL: 130 mg/L. Totalhardness (asCaC03 mgll): 40-42. Alkalinity(asCaC03 mgll): 40-42. Temperature (*C): 21.1-21.4. DO (mglL): 8.1-8.9. pH: 7.8-8.1. 1Specificconductivit(yt4@oslcm): 113-122. 4.0 TEST QA/QC QA/QC Criteria Mortalityincontrolexposuremust notbe more than 10%. The lowestDO concentratioanttest terminatiomnust be atleast60% of theair saturatiovnalue attesttemperature. Testdurationmust be 96 h. ReferenceSubstanceToxicityTest(thistestis optional). Data 5% mortality. The lowestDO concentratiomneasured was 91 % of theairsaturatiovnalue at 2 1.O'C. The testdurationwas 96 h. Controlchartforsodium chloridereference toxicittyestinigsin Raw Data Package. Sponsor: 3M Company Sponsor Study ID# U1464 5 ASci Corporation/ASci-Duluth EnvironmentaTlestingDivision Submittetdo3M Co.-5/98 ASCI StudyID# 5010-068 5.0 REFERENCES AmericanPublicHealthAssociati(oAnPHA). 1995.StandardMethodsfortheExaminatioonf Water and Wastewater. APHA, Washington, D.C. Girling,A.E., G.F. Whale, and D.M.M. Adema. 1994. A GuidelineSupplement for Determining theAquaticToxicityof PoorlyWater-SolubleComplex Mixtures usingWaterAccommodated Fractions.Chemosphere, Vol. 29, No. 12,pp. 2645-2649. OrganizationforEconomic Cooperationand Development (OECD). 1981/1993. OECD GuidelinesforTestingof Chemicals.OECD PublicatioInnformationCenter,Washington, D.C. West Inc.and Universityof Wyoming. TOXSTAT Wyoming. SoftwarePackage. Version 3.5.Laramie, USEPA. EPA ProbitAnalysisProgram used forCalculatingEC Values.Version 1.4. Sponsor: 3M Company Sponsor Study ID# U1464 6 ASel Corporation/AScl-Duluth EnvironmentalTesting Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 TABLE 1. ACUTE TOXICITY OF U1464 TO LARVAL FATHEAD NIE@NOWS (P.promelas) .......... 0 (control) 78 130 216 360 600 ... ...................... ........................... . ....... ...................fiyp_@U:M.. ams ms :,an:.:..dittht ........ 72 h h ........ 1 (5) 1 (5) 1 (-5) 1 (5) 1 (5) 1 (5) 1 (-5) 1 (5) 1 (5) 2 (10) 2(10) 2 (10) 7 (35) 14(70) 14(70) 15 (75) 12(60) 18(90) 16(80) 19(95) 16(80) 19 (95) 16 (80) Percentages are in parenthesis. LETHAL AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFI]DENCE INTERVALS 24-h LL50 48-h LL50 72-h LL50 96-h LL50 96-h LLIO 96-h NOEL 308 216 216 206 115 df 130 '95.%@::C dence .Ihlt6 238-380 168-264 168-264 162-247 66-150 Not applicable Values based on nominal testconcentrations. Sponsor: 3M Company Sponsor Study ]D# U1464 7