Document EqMvMRNZDpx45pkwoN5aMQ72b
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity:Perfluorooctanesulfonyflluoridem;ay also be referredto as POSF, or FX-8. (1-Octanesulfonylfluoride, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecaflCuAoSro-#, 30735-7).
Remarks: The testsubstance isa clearliquidofuncharacterizedpurity. Itisdescribed as FX-8 (cc842-2)washed sample.
METHOD
Method: ProtocolReference EAR(WAS 5/9/84 Test type: Not noted. GLP: No Year completed: 1984 Analyticalmonitoring: Dissolvedoxygen concentrations. Stock and testsolutionpreparation:Test substance (0.309g)weighed directliyntoBOD bottle5,0 mL deionizedwater was added, and the bottle mixed on a magnetic mixer for- 30-minutes. Bottlewas capped during
mixing. lnoculum source: Activatedsludge mixed liquorfrom the Metro wastewater treatmentplant- St.Paul/Minneapolis,MN. lnoculum holding: None - used the day itwas obtained. Element basis: Respirationinhibitioans determined by oxygen
consumption.
Method remarks: Aftermixing inthe BOD bottle,itwas noted thatthe sample settledto the bottom,formingsmall dropletsor beads. Some of the sample appeared to emulsify.
RESULTS
Nominal concentrations: 1000 mg/L testmaterialsolution.
Element value:
6.75-min IC50= > 1000 mg/L
Control response: Adequate - 6.5% differenceinrespirationrateof the two controls.
CONCLUSIONS
The testmaterialexhibitedno inhibitoreyffectat 1000 mg/L over a 6.75minute exposure.
Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St.Paul,Minnesota, 55133
DATA QUALITY
ReliabilityK:limischranking3. The method used was not agency approved. Thisstudy lackssufficiendtocumentation,characterizationf the testsubstance puritya,nd analyticamleasurement oftest concentrations.
REFERENCES
This studywas conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, Lab Request number B1229.
OTHER
Last changed: 5/18/00
3M EnvLronmentaL LaboratorS
Acute ToxLcLt@ of a Substance to ActLvated SLudR8 MLxed LLuor (SLUDGE-TOX) ProtocoL Reference: ER /WAS 5/09/ 4, Lob Request No. B1229
8-
76-
CD
Legend 0 DEIONIZED WATER CONTROLS - AVERRGE
A B1229--2 FX-EL C0842-2, WASHED, (1000 MG
IC50 >1000 MG/L
>< 40 C3 Li -3 CmD 3u-i
2-
0
0
1
2
3
4
TIME MINUTES
6
7
STOX. DAT
3M Environmental Laboratory
Acute Toxicity of a Compound (Substance) to.-ActivatedSludge Mixed Liquor
Protocol Reference- EAR/'WAS 6-09-84
-
Lab request no.
Date
Anslyst:,/",/Y,":@,y
The activated sludge mixed liquorwas collected from the St. Paul/Minneopolis Metro Wastewater Plant on
Sample description:
B/ 2 2
Preparation of stock -toli-tl;@
grams of sample were diluted to
ml
with dolonized reagent water. Date of preparation:
-TIME
min. sec.
O@CENTRATION CthT@@L
7, -7,
OF TEST
MATERIAL
TI ATED
o I @6
lcon ol
SLI GE MIXTURE
C -,,-7-n @ -
7.
c c
3.
Y -7--
COMMENTS:
T@ c
't
LC
OL
3-27-84
ENVIRONMENTAL
LABDRATORT STATUS
ALL RESULTS TENTATIVE
REPORT
LAB REQUEST NO. B1229
'EQUESTER NAME: ANW/EAR )EPT: 0535
)ROJECT NO: 9970012600 )ATE RECEIVED: 2-21-64 )ESC: FX-8 (CC 842-2)
CONTRACT LAB:
CONTRACT LAB COST:
0
34 E-L HOURS:
62
EST CMPLT DATE: 5-01-84
DATE COMPLETED:
3AMPLE IUMBER
NUMBER ONE.
TWO WILL BE GENERATED
FROR TWO EQUAL VOLUME WASHES OF SAKPLE
--------------------------------------------------------------------------------
SAMPLE
DATE
CODE
DESCRIPTION
TEST
RESULT
-------------------------------------------------------------------------------
1
FX-8
SKPL-PREP
COMPLETE
2
DASHED
SAMPLE
96-HR-LC50
C-BOD-05
#SEE REPORT
C-BOD-10
C-BOD-20
SOLUBILITY
BIDSORP
JEG-SPEC
SLUDGE-TOX
UPDATED DATA CONTRACT LAB
4-11-94
ENVIRON4ENTAL
LABORATORY
STATUS
ALL RESIJLTS TENTATIVE
RV-PORT
LAS REGIJEST NO. B1229
RE@@UESTER NAME: AN@/EAR DEPT: 0535 PROJECT MIJ: 9970012500 DATE RECEIVED: 2-21-94 DESC: FX-9 (CC 942-2)
CONTRACT LAB:
CONTRACT LAB COST:
0
3M E-L 9OURS:
47
EST CAPLT DATE: 5-31-84
DATE COMPLETED:
SAMPLE NUMBER
NUMBER ONE.
TWO WILL BE GENERATED
FRO.4 TWO EQUAL VOLUME WASHES OF SAMPLE
-------------------------------------------------------------------------------
SAMPLE
DATE
CODE
DESCRIPTION
TEST
RESULT
--------------------------------------------------------------------------
1
FX-8
S4PLmPREP
COMPLETE
2
WASHED
SAMPLE
96-HR-LC50
C-BOD-05
SEE REPORT
C-BOD-10
SEE REPORT
CmBODm2O
OSEE REPORT
SOLUBILITY
BIDSORP SLUDGE-TOX
4 = UPDATED DATA = C9NTRA:"T LA3