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AR 226 ~ 0782 ACUTE TOXICITY TO FISH (RAINBOW TROUT) IESTSUBSTANGE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L- 3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). 2R0e6ma(rLk-3s2:43). ThCeur3reMntpriondfourcmtaitoinonlotinnduimcabteers witaissa1.miTxhtuereteosft 0s.a6mp7l%ePiFsOFSC,117%.5s%oddiiuemthoyclteynlesugllfyactoel, b0u.ty0l4e%thseor,di7u8m.0la1u%rylwastuelrf,at1e,.303.%5%Suplotloynoexyfeotahmyelre,ne monooctylpheny ether, and 0.05% benzotriazole. cThhaerafcotlelrowiiznegd scounmcmeantrryataipopnlsieosftiompaurmiitxiteusr.eDwaittah imnacyomnpoltetaeclcyurately reflect toxicity of the fluorochemical component of the test sample. METHOD: 0000 Method: Notgiven. Type: Acute static. YGeLaPr:cNomopleted: 1974 Species: Salmo gairdneri Supplier: Not given. Analytical monitoring: Dissolved oxygen, and temperature Exposure Statistical period: 96-hours methods: LCs value calculated using graphic interpolation. Test fish age: Juveniles Length and weight: AAvveerraaggee wleeniggthht==308..91gm(mwet) Loading: Not given. Protreatment: None. Test conditions: - Dilution water: Dechlorinated city water, St. Paul, MN Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: Not given. Concentrations dosing rate: Once Stability of the test Exposure vessels: chemical Not given. solutions: Not noted NNuummbbeerr ooff friesphlipcearterse:pl1icate:Notgiven. WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e five plus study: a blank control DTiesmspoelrvaetduroexyrgaennger(a0n-g9e6 (h0o-u9r6s)h:our5s0):F 001466 44..0955..29 mmggl/LL ((c3o0n0tr0omlge/xLpoesxuproes)ure)" * 3000 mg/L (intermediate concentration) exposure values used because the highest two concentrations resulted in total mortality by 72 hours. m0 esw0 rs00000000000 Nominal concentrations: Blank control, 2000, 2500, 3000, 3500, and 4000 mg/L. Element values: 96-hour LCso = 2500 mglL Element values based on nominal concentrations RSuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTdhucetveadlounestrheepmoirxtteudraepapslydetsoctrhiabtemdixintutrheeaTnedstnot the fluorochemical proportion alone. conclusions The FC-206 96-hour LCs for rainbow trout was determined to be 2500 mg/L. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 pataQuALTY Reliability: Kiimisch ranking 3. Testing lacks record of methodology used and the documentation. The study lacks analytical sample purity was confirmation of the naomtopurnotpeorflfylcuhoarroacchteemriiczaeld and proportion in the solution. R0 EFER0 ENC0 ES 0000000000 This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, 1974. o0 we0 r 00000000000 Last changed: 6/28/00 001467 . 7001 Con wtan es Fh mciiagin7s MEE ENVIROANQMUEANTTIACLTEONXGIICNIETEYRIWNOGRKLSAHBEOERTATORY Bow CommCh Pros ct ( E=mil ` Type Test__S7xs7/c -9C lowe MaterialTeste 3293 Lor %/ > Test Organian_Rgip bony Tr0u7 Avg. Weight _. 7 3 Size_JIT Tm Date started___ 4/1/79 Date Completed 7/5/59 Time Started___ 9300 Pm Plotted Lgg Dilution WateP r3_S7T a Poutu CCTTl , c-fclechlorina/ 2500 mg/f LCloo = 2000 smal soo | Jan]soo[ Jae 100] Joo] 00|Jo Conc. 24 hrs * Survival 0.0 pH pp 48 hrs s Survival D.0 pH pp 72 hrs s Survival 0.0 pH pp 96 hrs ` Survival D.0 pH pp Ie aoe too|[4] 00| |sol100][97] 100| [2d ase[roo |[5a] too|[09] wo|[2] 0|fy spre ibe ||g so| [ad to] [asl so | ao] ens]ol wcoo [ |[efalsaoo[o |hf]aad =0[[TTa=al==|[-]]-] Aversge Temperature So' = so''- s0- 50 omaents: om m pC. a i i SSH& EEERFECT T T OT T E TE TEfTE oTEiEE oo 3 I em Te he 2 WH LE TT elpEle EE R IR E EE TT Z= c EE TEE & | glediriitesmie io Flin TTR mn CoE w Men mbr Tee ET H Ee POEL i or 1 in EEE] Se prick i rH ER poi ! Hh 1 ' ao t 0 1i 0 T20 so dl o S0H 60 70 80ri0 nio + survival wane 5 Ce 001468 Ce