Document EpzXkV00qevZ7O1K8K054ML0

Mass 328 463 AR226._ 328] -_-- wDuePonnt. 1n2090 TRADE SECRET Study Title H-25767: Static,DAacupthen,i4a8m-aHgonura Screening Test to AUTHOR: Barbra D. Ferrell, B.S. Robert A. Hoke, Ph.D. ~ STUDY COMPLETED ON: May 28, 2003 PERFORMING LABORATORY: E.L du Pont de Nemours and Company EHlakstkeolnlRLoaabdo,raP.t0o,ryBfooxr H5e0alth and Environmental Sciences Newark, Delaware 19714-0050 WORK REQUEST Novos {lll STUDY Cope Nowe Jil) -- DE -- CompanySanitized. Doss notconTtsaicnn S425n767 pSai,A 5ow --_ Be CERTIFICATION `We, the undersigned, from this study. declare that this report provides an accurate evaluation of data obtained veka Pa SDer(n o:iu o esta oes thstI SoeKkEwRE es44ft k a2e6uMayy o2w0es5 -- P -_-- Company Sanitized. Dose not contain 75.4 caf Sa oe ps in ~ STUDY . INFORMATION Te Haskell Number: 25767 Composit | om KnownImpurities; Physical Characteristics: White liquid ~ Stability: en The test substance appeared to be stable under the conditionsof the study; no evidence of instability was Sponsor: WELiLlmdiungPtoonnt,dDee`lNaewmaoruers19a8n9d8Company USA. Study Initiated/Completed: May 13, 2003/(see report cover page) `CompanySaniized. Doosnotcontain Tscacs ScHSrc-or2neSeiTn6vi7ng:gTTSeetsastttiicto,oDADcoauppthehn,vi4ai8-maHamogeunrgana____ -- DuDuoPwonita1o26s9o0 SUMMARY `dTehteeramciutneedtoixnicaintyunoafeHra-t2e5d7,6478-thootuhre,wsatatteirc ftelseta., Daphnia magna (less than 24 hours old) was `mTehaensttuedmypewraastucroenodfu2ct0e.d7wiCt(hr4ancgoenocfen2t0r.a5t-i2o0n.s8oCf).H-2O5n7e6t7esatncdhaadmibleurtiwonaswautseerdcpoenrtrtoelstat a ccoonntcreonltraantdio1nmwgit/hL1te0sttecsotnocregnatnraitsimosnisnoleuatcihoncshawmebreer.cleBaarsaenddocnolvoirslueaslsoabtsteersvtasttiaortn,s,Tthhee water c1o0ncmegn/tLratteisotncosonlcuetnitornatwiaosn csloelaurtiwointwhaassucrlfeaacrewiftihlmaasnudrftaecste mfaitlemarialteostnsttharet.solTuhteio1ns0u0rmfagc/eLatnedst baostutrofamoceftfeislmt acnhdamtebsetrmaattetreisatlstoarnt.thTehseolu1t0i0o0nmsgur/fLacteesatncdonbcoetnttormaotfitonesstolcuhtiaomnbewrasatctleosutdsytawrti,th All water quality parameters were within acceptable limits during the exposure. Eanxdpo1s0u0r0eomfgd/aLphrensiudlstetdo itnh0e,w0a,te0,r 7c0onatnrodla1n0d0n%oimmimnoabilliHt-y2,5r7e6s7peccotnivceelnyt,raattitohnesoefn1d,of104,8 1h0o0u,rs. Nnoomiinamlmcooncbenotirrsalutbiiolnettchayaulsienfgfenctosiwmemroebsieleniatityn ttheestweantderwcaosnt1r0omtge/sLt.orTghaneislmosw.estThneomhiingahlest --_ ccoonncceennttrraattiioonnscwaeursienugs1ed00fo%ricmamlocublialtiitoynoaftLeCstseonvdalwuaess.1000 mg/L. Nominal H-25767 "Theresults are summarized as follows: Nominal concentrationsof H-25767, mg/L | dilution water control, 1, 10, 100, and 1000 4fo8r-hHo-u2r57L6C7s,ob(a9s5e%d oconnnfoimdienncael interval) | 69 (28 0 210) concentrations, mg/L* H-25767 using the ewxahtiebritfeleda,mDeadpihunmicaomnacgenraf(olresasqutahtainc2h4azhaorudr"sionlad)n. unaerated, 48-hour, static acute test a FPEenlrvtiierero,nsmWe.hnHat.wanladanMMdotanWrieetibonrereri,nOgrCg1a1a,nndEisdSmsus.p.po1rE9tP8A5LJ)aG.b0oMr0ea4tt-oh8ro5y-d,0sC1if3no.crinMnUeanatisit,uerdOihSnitoga.ttehesAEcnuvtireonTmeuntsalioPfrcoEtfiefcuttieonynsAtgoency, b mSemtrhcohdeska,ndJ, CevallueatimonReo,fndMaottrac.socE,knv,iRro.namnedntRaalbeTrots,icWo.lo(g1y99a3n).dRAissskesAssisnegssemceonlto,giAcaSlThMaSzaTrPdu1n1d7e9rTSCA: ~~ (PhWi.lGa.deLlaphnidais, 1.5. Hughes, nd M.A. Lewis, Eds.)pp 22.39. American Society for Testing and Miaterils - `Company Sanilzed. Doesnotcontain TSCA CBI SHec2iSc7e6n7i:ng TSetsatte1, ADcaupthsn,i4a8-mHaogunra --~ MATERIALS AND METHODS DuPont-12690 A. Test Solution Preparation Tsoelsuttisounbssotfan1c,e 1s0o,lu1t0i0o,nsanwder1e0p0r0empagr/eLdwbeyrdeirpercetpaadrdeidtbioynaodfdiHn-g2t5h7e67aptporodpirliuattieonawmaotuern.t Tofetsetst maantder1imalg/toLHteLstWcoWnciennt1r-aLtiboenakseorlsutainodnsstwierrreincglfeoarr aapnpdrocoxliomralteeslsyat60temstinsutatrets.. TThhee1w0amtger/Lcotnetsrtol scoolnucteinotnrawtaisonclseoalrutwiiotnhwaassurclfeaacrewfiitlhm aasnudrfteasctemaftielrmiaatltoensttshtearts.olTuthieon1s0u0rfmagc/eLantedstbocotntcoemnotfratteisotn acnhdamtbesetrmaattetreistalstoarnt.thTehseol1u0ti0o0nmsgur/fLacteesatncdonbcoetnttormaotfiotnesstolcuthiaomnbwearsatclteosutdsytawrit.th a surface film B. Dilution Water aDnildutsieoanlewdattoerbeodrriogcikn.ateTdhferhoamrdthneesHsaosfketlhleLHabLoWraWtorisyawdejlulswtehdictho aispp3r7o8x-ifemeattedleycp10a0n-d14is0cmags/edL athrCoauCghO;abgyretehnesaflnodfwi-lptreorptoortrieomnoedveaidrdoint,iaonnodffCialCtelr.ed tThhreouHghL5W0+W, 1i0s-,thaenndaSeruatmed,fipltaesrssetdo remove particulates. The water is heated or chilled as appropriate and distributed through aged ~~ pcaotliyovnisn,ymlecthalso,ritdoetaplipoirngga.noTchhleodriilnuetiaonndwaotregraniospahnoaslpyhzaetdetpweistciecyiedeasr,lyafnodr pmoaljyocrhlaonriionnasteadnd biphenyls. The dilution water meets OECD" and ASTM specifications. C. Test Organism Culture cDualptluirneiianimtaiagtnioan)wwerheicrheacroendtaatinHeadsk1e0l0l0LmabLoorfataocrryatiend,10fi0l0t-ermeLd PHyLreWxWbheealkderast (201.08peCr. beaker at D(satpahnndiadrdsiwzeedretofe1d70o0ntaod2a1il0y0bmagsi/sLwtiotthalaSyoelaisdts,) caenrdeatlhelegarveeesnaanldgat,roSuetlecnhaoswtr(uYmCcTa)prmiicxotrunruetum, a1t0a0.r1a-t0e.o2fm6g2t,o5t0a0lcoerlglasn/imcLocfacrubolntupreermdeapdhinuimd.peTrhedacy.ombNienoantaitoensoufseYdCiTn tahnisdtaelsgtawiesreequleisvsaltehnatn p2a4rhenoturdsapohlndiadsn,d rweesrpeectciovlelleyc.tedSifcrkonmestsh,ei7n*j,ury1,0a%ndaanbdno1r2m%ablriotoidesswoefr2e1,no2t8,saenend a2n8d-deapyhioplpdia `were used not being for testing produced by the parent daphnids. during the 48-hour pretest period. NDoapadhunlitaimmmaogbnialiwteyrweaisdesneteinfiiend the by cultures labels on the culture beakers and test chambers. --~ CompanySanitized. Doss notcontain TSCA CB eerie: S SBc-r2e5e7o n6i7n:gc TSetsattice , ADcaupg then,i4a8T-mHaogune ra _sioDaplviameDgDiuoPomnnitz1ea 29w90o --~ D. Test Methods `Fcoounrcennotmraitniaolnscownecreent1r,a1t0i,on1s00a,ndanaddi1l0u0t0iomngw/aLteHr-2co5n7t6r7o.l were used in this study. The nominal Pyrex" used as bteesatkcehrasm(b2er5s0.-mLO)necornetpaliinciantge 2te0s0tcmhaLmobfetreswtassoluusteidonp(e6r.t8e-sotmcotnecstenstorlauttiioonn depth) with were 10 daphnidsineach chamber. The test chambers were covered with a glass plateduringthe test. `duDraipnhgnitahemategstn.aAndedointaitoenso,fldesaspthhnaind2s4tohotuesrtssoolldu,tiwoenrsewuassediniintitahtiesdsatfutdeyr.miDxaipnhgnoifdtshweetreestnot fed scroiltuetriioonnsfwoarsthceoemfpfleectte(di.mmIombmiolibtiyl)itwyaasnadlbaechkavoifroeraacltoibosnertvoaatpipolniscawteiroen moafadegednaitllye.stTihmuelus. Athere4c8i-rhcouluartiensgt waattaeprpbraotxhimwaatseluyse2d0.t7omCaiwnittahian rmaenagneotfem20p.5erat10ur2e0.i8ntCh.e tAestphcohtaompbeerrisodduorfing i1n6chlouduerds 3li0ghmti(nauptpersooxfitmraatnesliyti5o6na4ltloig7h4t0(3L4uxt)oa4n5dL8uxh)ouprrsecdeadriknngeasnsdwafsolelmopwilnogyethdew1h6i-chhour light interval. cDoinstsroollvaenddoxteysgtesnubcsotnacnecnetrcaotnicoenn,tprHat,ioannsd(tTeambpleera1t).urTehweesreemmeeaassuurreemdenitnsalwlerrepelitcaakteensoatfttehset sari, ~~ daunrdiantgttehset etensdt aonrdatwteortealdiimsmpoobsieldioftyininaanraepplpircoaptreiactheammbaenr.nerTeastttessotluetnido.ns were not aerated RESULTS AND DISCUSSION Tmheeansttuedmypewraastucroenodfu2c0t.e7dwCit(hr4ancgoenocfen2t0r.a5t-i2o0n.s8oCf).H-2O5n7e67tesatncdhaadmibleurtiwoanswautseerdcpoenrtrtoelstat a ccoonntcreonltraantdioInmwgi/thL 1te0sttecsotnocregnatnraitsimosnisnoelautcihoncshawmebreer.cleBaarsaenddocnolvoirslueaslsoabtsteersvtasttiaortn.s,tThheewater `1c0onmcgen/tLratteisotncsoonlcuetnitornatwiaosn scloelaurtiwointwhaassculrefaarcewiftihlmaasnudrftaecste mfaitlemraialteostnsttharet.solTuhteio1n0s0urmfga/ceLatnesdt baostutrofamocefftieltm acnhdamtebsetrmaatetreisatlstoarnt.thTehseolu1t0i0o0nmsgur/fLacteesatncdonbcoetnttormaotifotnesstolcuhtiaomnbewrasa ctleosutdsytawritth All water quality parameters were within acceptable limits during the exposure, Eanxdpo1s0u0r0eomfgd/aLphrensiudlstetdoitnh0e,w0a,t0e,r7c0onatnrodl 1a0n0d%noimmimnobaillHit-y2,5r7e6s7peccotnicveelnyt,raattitohnes eonfdo1,f104,8 1h0o0u,rs (hiTgahbelset2n).omNinoalimcmoonbcielnittryaotrionsucbaluesthianlg enfofeicmtsmowbeirleitsyeeatn tiensttheendwawtaesr control test organisms. 10 mg/L. The lowest The cnoonmciennatlractoinocnesntwrearteiounsecdaufsoirncgal1c0ul0a%tiiomnmoofbiLlCitsy at test end values. was 1000 mg/L. Nominal H-25767 --~ FompanySaniized.DossnotcontainTscacal --TT TTT TT Se SHcArSoTe6n7i:e ng TSetsattic1,b ADcauptlei,e i4a8m.Haoge unra DwuPeonnt1n2990 --~ uHs-i2n5g7t6h7e ewxahtiebritfleeda,mDeadpihunmicaomnacegrnnaf(olreassqutahtainc2h4azhaorudr"sionlad)n.unaerated, 48-hour, static acute test REFERENCES 1. OTersgtainnigsoatfioCnhefmoircEaclos:no2m0i3c,C1o7-JOupleyra1t9i92o.n and Development (OECD). Guideline for the 2. CAomnedruicctainngSoAcciuettye fToorxiTceisttyinTgesatnsdwMiattherFiiaslhses(,AMSaTcMr)oi.nve(1r9t8e8b)r.ateSst,anadnadrdAmGpuhiidbeiafnosr. E729-88a. Annual Book ofASTM Standards, Vol. 11.04, ~ mVSemGtrhc.hoedLks,annJd,diCel,veaml1.5e.unaHttuisgo,nhoRefs.,dMaaonrtdc.oMEc.nkAv,.irRLo.enwmaiensdn,tREaadlbseT.ro)tx,pipcWo.2l2o(:g13y99a9.3n)d.ARmieAsrskisecAsassnsieSnsgsmeoecnotlc,ofgoiAricSTaelThseMatziSatnTrgPdsyu1nn1d7d6Me,arteTrSiCaAl,: Philadelphia. eT ---- Company `Sanitized, Do0s notcontain Tp cg HS-c2r5e7e6n7i:ng TSetsattict,oADcaupthen,i4a8-meHaoguenra -- DDuuPwoontm12n6s90 TABLE | DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES. Nominal Test Initiation (0 hours) H-25767 Concentration | Dissolved Oxygen (ng mg p Test Completion (48 hours) Dissolved Oxygen (mg/L) p Water Control 84 Lo 84 78 79 10 100 84 84 79 79 1000 84 77 85 86 78 78 87 79 87 7.9 86" 74 a Measurements taken at24 hours due to total immobility in atest concentration. --~ TABLE2 IMMOBILITY OF Daphnia magna IN AN UNAERATED, WITH H-25767 STATIC, ACUTE, 48-HOUR TEST Nominal H-25767 Concentration (mg `Water Control 10 10 100 1000 | NumNbuemrboefrToefstDeOardgaTneisstmOsragtaSntiudsymsS/tart [24H|odsw Howss| 10 010 10 0/10 10 10 010 710 10/10 1010 ~~ pyBiizsd,Does not containTSCACHL -Y