Document EdvbYwxpZx88aJKZ6x4Vd9ZDV
AR 826 -- 026
ACUTE TOXICITY TO FISH (FATHEADMINNOW)
IESTSUBSTANCE
~~~
0
Identity:
A mixture containing perfluorooctanesulfonate, which may also
be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 11,22,3,3,4,4,556,6,7,7,8,8,8heptadecafiuoro-, potassium salt, CAS #2795-39-3)
Remarks: The 3M production lot number was not noted. The test
sample is FC-600. Current information indicates it is a mixture of 1.0%
PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium
octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate,
0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chioride, 0.80%
starch, and 0.05 % benzotriazole.
-
The following summary applies to a mixture with incompletely
characterized concentration of|impurities. Data may not.accurately reflect the toxicity of the fluorochemical component of the test
`sample.
M0 ETHOD: 000
Method: Not noted.
Test type: Acute static
GLP: No
Year Completed: 1975 Species: Pimephales promelas
Supplier: Not noted
Analytical
Exposure
pmeorniiotdo:ri9n6g-:hoTuermsperature,
pH,
DO
Statistical methods: LCs, values graphed. 95% confidence intervals calculated by probit analysis.
Test fish age: Not noted
Average Length and weight: Length = 2.0 inches
=
Loading: Not noted
Weight = 1.5 grams
Pretreatment: Not noted.
Test conditions:
Dilution water: Carbon-filtered water, City of St. Paul, MN.
Dilution water
Lighting: Not noted
chemistry:
Not
noted
_
Stock and test solution preparation: Test solutions were created by.
direct weights additions.
Concentrations dosing rate: Once Stabilityofthe test chemical solution: Not noted
Exposure vessels: Not noted.
Number of replicates: One
001992
Numberoffish per replicate: 10
NWautmebrecrheofmicsotnrcyendturraitnigontsh:e 5stpulduys:a blank control
pH
ran7g.e0(-07-.926
hours): (control
exposure)
7.37.4 (2,000 mg/L exposure)
Temper7a2tuFre range (0-96 hours):
Dissol5v.e7d-o7x.y0gmegn/Lra(ncgonetr(o0l-9e6xhpoousrusr)e):
4.0-5.7 mglL (2,000 mglL exposure)"
w*eVraeluuessedforbethceau2s,e00t0otmalg/mLorteaxlpiotsyuorcecu(rthrierdd hinigthheeshticgohnecsetntration) `concentrations tested.
R0 ESW1s000000
Nominal concentrations: 4,000 mg/L
Bk
control,
1,000,
1,500,
2,000,
3,000
and
Element value: 96-hour LCs =1,700 (1,465 ~ 1,972) mg/L
Element value based on nominal concentrations.
RSuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadluoen rtehpeomritxetduarpeplaisedsetsoctrhiabtedmiixnttuhree Taensdt
not the fluorochemical proportion alone.
c0 oncl0 usi0 ons 0000000000
T1,h7e00FCm-g6/0L0w9it6h-haou9r5L%CsC,onfforidPeinmceephIantleersvaplroofm1e,l4a6s5wtaos1,d9e7t2ermmgi/nLe.d to be
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, Minnesota, 55133
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p0 araqu0 at 00000
Reliability: the method
Kiimisch followed.
rTahnekisnagm3p.leThpeuristtyuwdyaslancokts
pprrooppeerrlydocchuamraecntteartiizoend
of
and the study lacks analytical confirmation of the amount of fluorochemical
proportion in the solutions.
oo.
This study was conducted by 3M Company, Environmental Laboratory, St.
Paul, MN, 1975.
001993
o0 mer00000000
Last changed: 6/28/00
001994
. 730101
ENVIRONMENTAL ENGINEERING LABORATORY
AQUATIC TOXICITY WORK SHEET
Se--------
t : vee Test Hou Static
toseorganionF0lond]Minn)
Material Tested f=dag
wu. we _L5o_ sit2Tndh
Date Started.
F-R5-75
Date Completed
3-29-72
Time Started.
2:00
Plotted gg
Dilution econCathe Qleted--city quer
--------
PimePhalee pomalas
(EmTeal tPoSo[7A2]P70] too2Fralgs] mo ZAE.E 00 Tle] Conc.
24hrs
` Survival
D.0 pl PI
48 hrs +
Survival
72 hrs
0.0
`
pp Survival
0.0 Pt
96 hrs
`
D.0
usvival p PP
2000] 0 Flea] 90 fsol solala]40Jrslad
|GPae| 0T|s 4 |0] |e0l] 21| 07| TA Minvew_eftect Contalnar = c------
100 HTHEE TT
Tr
w drhmeel
Cin hei
iEe
:
F0oor-eiedibrnC E EE T
nae
ae
I
i
CTT TTI
Ci E i Ce E A ii EACbp rilm 0 10 20 30 40 50 60 70 80. 90 100
Survival
HA
Lo
{00199544i
L-
ENVIRONMENTAL ENGINEERING LABORATORY
Lo.
AQUATIC TOXICITY - PROBIT ANALYSIS WORK SHEET
(
Date Started: Tine Started:
__$- 95-77 2.00 _MO@M)
Material Tested: [TC -60p Test Organise (Fathesd minnow)
pe Test: (Cbvimenrsim)(static) Avg. We. LS (ram) Avg. Sie 3.0 (inch)
EPwixlpuoexsnut:re(Pcearriboodn:-filt.oSe.P9.6ClttoyurWer.) @ADnaatteysCiosmplbey:teWd:Y, Zlnobotn 5-33-19
No.PlootfteDdoses | conc.
1
lowl
sMeorrtavleitdy ||
--0
EMoxrpteacltied 4
CoVrarleucetse|d
0Gb-sekrved- | Expected|
tCooncthriib(uftrioonn Nomograph #1}
7Teo pe Tag
2 3
hsodl oodl
gogo| Tee--]
4p
6p TT----1--
5
6
rT ee
7
L|
11] :
K=__4 mnimsls/bosen. [0 Total contribution to chi
Total Animals =
40 (chi?) contrib. to chi x Tot. Animals
= ohn
Loh'eteroeg-eBnu0bo9u)s. for (vdi)s loss th2n __ 5.d9e9gree; othferferfeoerdeo,n t(hferodwaTteablaere7)not s+=iS_gnihf5i,o c0a9ntly
Lg=
__ndon
95% Confidence Limits for LCey
ll go =__\loo
yg =
\a.Soug/l
Stope function (5) ICe,/ICsy + 100/104 Fev ry Mev ree \29
Total number of animals used between 16% and 84% expected effects (N)s _ CLO
fLeigco,/fL=Cg7 y = lo, wer limit =(\1agog/\\p= = (from No h #2
3
Legg LC
X =
fLCgy
=
upper limit AN00
=\1og X\3, = (95% confidence
limits
Az)
(1) cDoonnsoectutliivset m1o0r0e% etfhfaencttsw.o consecutive 0% effects or more than two
-
(2) (9)
Expected valus for any dose Corrected value for each O%
should bo greater than 0.01% or or 1008 effect (from Table 1).
less
than
99.99%,
ES 5
: There is a 95% chance that tho true value of LC, lies within these limits.
4
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