Document Edrwj2L2zyom37O66Jak7Lwr0
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe
referredto as PFOS, FC-95, or as a component of FC-203CE. (1OGtanesulfonicacid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 519. The testsample isFC203CE (2/21/89).Currentinformationindicatesitisa mixtureof 1.84% PFOS, 30% diethyleneglycolbutylether,60.23% water,2.56% Sultone foamer,3.1% sodium octylsulfate2,.1% polyoxyethylenemonooctylphenyl ether,0.12% sodium laurylsulfatea,nd 0.05% tolyltriazole.
The followingsummary appliesto a mixture with incompletely charactedzed concentrations of impudties. Data may not accurately reftct the toxicityof the fluorochemical component of the testsample.
METHOD:
Method: OECD Method 203 and U.S. EPA Method EG-9
Type: Acute staticrenewal
GLP: No
Year completed: 1989
Species: Pimephalespromelas
Supplier:AquaticBioSystems,Inc.,FortCollinsC,O
Analyticalmonitoring: Temperature,pH, and DO
Exposure period: 96-hours
Statisticamlethods: Not given.
Test fishage: Juveniles
Length and weight:
Average length 19.9mm
Average weight 0.06g (wet) Loading: 0.20g fish/L
Pretreatment:None
Test conditions:
Dilutionwater: Carbon-filterweedllwater
Dilutionwater chemistry:
Temperature: 20 *C
DO:
9.6 nig/L
pH:
8.0
Lighting: Not given.
Stock and test solution preparation: The testmaterialwas added directly
tothe dilutiownater on a weighttvolumebasis.
Concentrations dosing rate: Daily
Stabilityof the testchemical solutions:Not noted
Exposure vessels: 4 L Pyrex glasscylindrictaalnks containing3 L of
solution.
Number of replicates:2
Numberoffishperreplicat1e0: Numberofconcentrationosn:eplusa blankcontrol Waterchemistrdyuringthestudy:
pH range(0-96houm): 7.9- 8.0(contreoxlposure) 7.9- 8.0(1000mg/L exposure)
Temperature range (0-96houm): 20-21OC Dissolvedoxygen range (0-96houm):
7.7- 9.6mg/L (controelxposure) 3.8- 9.6mg/L (1000 rng/Lexposure)
RESULTS
Nominal concentrations: Bk controland 1000 mg/L
Element values: 96-hourLC50 = >1000 mg/L
Element valuesbased on nominalconcentrations.
Remarks: Testingwas conductedon a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportioanlone.
CONCLUSIONS
The testsample 96-hour LC50forfatheadminnow was determinedto be >1000 mg/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilitKyJ:imischranking2. Testingmeets thecriterifaorqualittyesting. However, sample puritywas notproperlycharacterizeadnd thestudylacks analyticaclonfirmatioonf theamount offluorochemicaplroportioinnthe solution.
REFERENCES
This studywas conductedby the3M Company, EnvironmentalLaboratory,
St.Paul,MN, Lab Requestnumber G 1358-1,1989.
OTHER
Last changed: 6/26/00
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Test methodology conforms with the following two test protocols: (1) OECD 1203 "Fish, Acute Toxicity Test".
(2) US-EPA IBG-9 "Fish Acute Toxicity Test".