Document EdrZJm6RBzGKQDyvg7MMQMk4
Quality Assurance Project Plan M ulti-City Study Sampling Task (N1Q0672)
R226-0077
Amendment 1 to Revision No. : 1 Effective Date: Junel6, 1999
Page 1 o f3
Amendment 1 to the
S j 'S S
Quality Assurance Project Plan and Associated SOP's
for
Empirical Human Exposure Assessment Multi-City Study Sampling Task
To (Confidential)
June 16,1999
Prepared by
Battelle 397 Washington Street Duxbury, MA 02332
(781) 934-0571
APPROVALS
Donald Gunster Sampling Task Leader
Rosanna Buhl Battelle Sampling Task QA Officer
-- Marcia Nishioka ' Battelle Project Manager
----
Date
Date .7/ .y ? i .
Date 7 -4 -4 4
Date
Date
000491
Quality Assurance Project Plan Multi-City Study Sampling Task (N100672)
Amendment 1 to Revision No.: 1 Effective Date: Junel, 1999 Page 1 o f3
Amendment 1 to the Quality Assurance Project Plan and Associated SOP's
for
Empirical Human Exposure Assessment Multi-City Study Sampling Task
To (Confidential)
June 16,1999
Prepared by
Battelle 397 Washington Street Duxbury, MA 02332
(781) 934-0571
APPROVALS
Donald Gunster Sampling Task Leader
Rosanna Buhl Battelle Sampling Task QA Officer
Marcia Nishioka ' Battelle Project Manager
Susan Abbg Battelle Program QA Officer
Date
Date
Date
7A A \
Date
Date
000492
Quality Assurance Project Plan Multi-City Study Sampling Task (N100672)
Amendment 1 to Revision No.: 1 Effective Date: June16, 1999 Page 2 o f3
Objective
The objective o f this amendment to the QAPP and SO P's is to document changes to several processes that have occurred within the field program o f the Multi-City Study. The initial field survey resulted in several issues that require either amendment or inclusion into the defined protocol for the field study.
Protocol Changes
Section 11.1.4 along with SOP M3-F-6 describes the labeling of samples collected dining the study. In addition to the samples highlighted in both of these documents, labels will be produced for `Trip B lank' samples.
The QAPP describes the collection of samples for the Battelle Columbus facility only. A t the clients request samples will be collected for two addition laboratories. The laboratories include the clients `in house' environmental lab and a third party validation lab. The additions of these unexpected samples triple the number o f containers collected during field activities. To ensure accurate sample control a labeling nomenclature system has been implemented. Samples for Battelle will contain a numerical identifier that corresponds to a given matrix followed by a `B '. Samples for the client will contain the same numerical identifier but w ill contain a `K ' following the number. Samples for the validation lab w ill also contain the same numerical identifier but will contain a `H ' following the number (ex. POTW Effluent ID: MC-101B, M C-l`01K, and M C-101H).
Section 12.2 o f the QAPP indicates that samples will be shipped at the end o f each day following collection. The logistical and scheduling difficulties associated with collecting over one hundred samples in a given day at various locations will, in most cases, precluded this from occurring. The amendment to this process is to store the samples on ice overnight and ship them as early as possible on the following day. Also, the shipment of samples on a Friday during a survey REQ U IRES the prior scheduling o f receipt on Saturday at either the destination facilities (OH or MN)or the Duxbury facility. I f samples are shipped to Duxbury, then arrangements must be made to send the samples to the destination facilities on the next business day.
SOP M3-F-4.0 Defines the labeling requirements for the spiking ampoules. The requirement is that each ampoule will be labeled individually with several pieces o f information. The actual size o f the ampoules precludes all this information from being recorded directly on the ampoules, therefore the information m ust be recorded separately and attached. To facilitate efficient and accurate labeling and spiking, a different approach will be initiated. The ampoules containing each type of spiking solution (polar and neutral compounds) will be stored and shipped in separate containers (i.e., ampoule storage container or standards storage box). On the exterior of the container all required information will be assigned. On the individual ampoules, only the name o f the compound class, e.g., polar or neutral (for polar compound spiking mix or neutral compound spiking mix) and the intended matrix for
000493
Quality Assurance Project Plan Multi-City Study Sampling Task (N100672)
Amendment I to Revision No.: 1 Effective Date: June16, 1999
Page 3 o f3
introduction will be affixed. This process will reduce the time required for labeling and
spiking while ensuring the accurate introduction of the proper material to the sample.
SOP M3-F-7.0 Section 3.0 describes the procedure for collecting water and sludge samples. These procedures indicate a step that requires the sample container to be wiped with methanol prior to and following sampling. This process adds hours to sample collection procedures. Following the initial field survey a discussion was held between the project manager and several team members to determine if the reduction in potential contamination warranted such a procedure. The result o f this discussion is that this procedure will not be continued during subsequent surveys. The use o f gloves during collection, and the prohibition of materials containing possible contamination sufficiently reduces the possibility o f contamination.
000494.
MULTICITY FIELD QAPP AMENDMENT 2
FISH and MICROLAYER WATER SAMPLING PLAN
000495
Quality Assurance Project Plan Mulct-Ctty Stuffy Sampling Task (N100672)
Amendment 2 in Revision No.: J Fffectiva 1>ate: July 20,1999
Page / o f 17
Amendment 2 to the Quality Assurance Project Plan and Associated SOP's
for
Empirical Human Exposure Assessment Multi-City Study Sampling Task
To (C on fiden tial)
July 23,1999
Prepared by
Battclle Marine Sciences Laboratory 1S29 West Sequitn Bay Road Sequins, WA 98382 360-681-3660
APPROVALS
Donald Gunster Sampling Task Leader
Rosanna Buhl Battclle Sampling Task QA Officer
Marcia Nishioka Battette Project Manager
Susan Abbgy Baitelle Program QA Officer
Date bate Date Date Date
000496
Euf< y^surance,
O bjective T k objective o fU aoaittaffl( ^ .
;fKgfiajandmicrolayetsa^ SC&^ - PiSrt"ftlc/ ***W
Protocol Changes Table 3 is amended below to include microlayer and bulk water samples.
Table 3. Sample Types, Sampling Devices, aw l Sample Votame*
>v
V olum ecy^A tiw uuteollectcd
D rtnklogW ^ter
Surface Water source (3 locations - 500 ft apart)
Sample bottle"
l L from each location; bulk water, not surface
Sediment from surface water source
Crab sampler or scoop
200 g from top 3 cm
(3 locations, match w/ water sample)
Plant intake
Sample bottle
ll
treated water from plant
Sample bottle
it
Tap water from retail establishment
Sample bottle
it
(from 3 locations in city)
Microtaycr from surface water
G lassplate
1"L ' ' -------
Bulk water from surface water
Sample bottle
1L '
Water from groundwater source"
Sample bottle
I t ............
bibU dy-O weed T reatm ent Work* (POTW )
iW ^hiori& etion rattuem (if avertable)
Sludge Effluent
Semple bottle Scoop Sm pkbottie
1L 1508
1L
J'
Leachate
ParttcuUtcs (glass fiber filter) V tpor 6>oiyuretfaanc t o m ewtrtgei
Ground beef(3 stores) Pork chops with bone (3 stores) Boneless, skinless chicken breast (3 stores) Portc-and-beefhot dogs (3 stoics) Regknal fish (3 stores) Eggs (J stores) Whole milk in plastic or cardboard container (3 stores) White bread (3 stoics) Promo green beans in plastic bag (3 stores) Fresh apples ( i stores)
1 Sample beetle
U rban A ir
.
G eaenl M ew Work*
Model PS-J air sampler
L o ealF o o d S u p p ty
Store packaging
Store packaging Store packaging
Store packaging
Store packaging Store packaging Store packaging
Store packaging Sts packaging
tere packaging
\ 1L
12 bat2S 0 U tain )2hat2S0U m ht
lib I lb(~!ichops^ t lb ( i whole breast)
l lb ( l package^
1 lb (lieted) 6 larg iq t
TS3f
I J*g
1 i t (at least 2 apples)
000497
Quality Assurance Project Plan Multi-City Studv Sampling Task (N i00672)
Organic bread or flour {1 store)
| Store packaging
Fish
finfish (electroshock) from surface I Smith-Root Model 12
water source (above)
1backpack elearoshocker
(a) Sample bottles will be ESS predefined, certified polyethylene bottles
(b) Not planned for Multi-City Study
Amendment 2 to Revision No.: 1 Effective Date: July 20, ]999 Page 3 o f 17
1 lb or 1 loaf
200 5 (minimum)
* Table 4 is amended below to include bulk water, microlaycr, and fm fish samples.
Matrix Surface Water (one water body)
Microlayer water Bulk water
Table 4. Sample Types and Numbers at Sampling Locations at Each City
Analyste TorSite! S a m p l e '
Sample Volume
.Analysis for -;
. .i___ J.:......................................... ....Me*?.- Site 2 Site 3 'Sites 2&3 Samples
Sample: P&N residuals (P & N)
1L IL IL P&N
Sample: Total PFOS (in duplicate)
11.
Field duplicate & laboratory duplicate: P
1L
Field spike: P&N
1L
Laboratory spike: P&N
1L
Field Blank: P& N
Field Blank: Total PFOS . Sample: Total PFOS (in duplicate)
1L
Field Blank: Total PFOS
Sample: Total PFOS (in duplicate)
1L
Field Spike: Total PFOS
1L
Sediment
Field duplicate & laboratory duplicate: P
Mg
Field spike: P&N
Laboratory spike: P&N
Drinking
Field Blank: P & W '
1L
water
Field Blank: Total PFOS
1L
(plant
Field duplicate & laboratory duplicate: P
1L
influent)
Field spike: P&N
1L
Laboratory spike: P&N
i 1L
Field Blank: P& N
Drinking
Sample: P&N
1L
water
Sample: Total PFOS (in duplicate)
1L
(piant outflow)
Field duplicate &!aboratory duplicate: P Field spike: P&N
IL 1L
Laboratory spike: P&N
1L
Tap water
Sample: P&Pi
1L
(retail outlet) Sample: Total PFOS (in duplicate)
1L
Field duplicate & laboratory duplicate: P
1L
Field spike: P&N
1L
Laboratory spike: P&N
1L
Field Blank: P& N
Landfill
Samples and laboratory QC (no field spikes): P &
1L
leachate
N; Total PFOS
Field Blank: P& N
TOTW-- influent
Sample and laboratory duplicate: P& N Sample: Total PFOS (in duplicate)
1L 1L
POTW
Samples and laboratory QC (no field spikes): P &
2i
sludge
N; Total PFOS
Field Blank: P& N
POTW
Sample and laboratory duplicate: P&N
IL
effluent
200 g 200 g P&N; Total PFOS IL IL P&N 11
000498
QvolUyAssurance Project Plan Multi-City StudySampling Task (N100672)
Amendment 2 to Revision No.: l Effective Date: July 20, 1999
Page 4 afY l
Sample: Total PFOS (in duplicate)
1L
Field duplicate: P & N
Field spike: P & N
1L -
Laboratory spike: P& N
1L
Field Blank: P& N
Urban air
Sample: P&N
Filter/PUF
Field spike* no air Field Blank: P& N
Filter/PUF Filter/PUF
Food Samples and laboratory QC
All All All P&N; Total PFOS
(no field blanks/fieid spikes): P&N; Total PFOS
Finfish
Sample and laboratory QC
2
Organic flour samples and laboratory QC (no field blank/ficldspike): P&N; Total PFOS
Trip Blank MtUiQ water P& N
lib IL
Trip Blank MilliQ water: Total PFOS
1L
F= polars: PFOS. POAA, PFOSA, PFSAA, M556; N - neutrali: Et/Mc of FOSE-H, FOSE-CI, FOSE-Amide
Table S is amended below to include bulk water and microlayer samples.
Tabic S. QC Samples by Matrix
Matrix and
Field Sites FieldRep': Lab
Analysis Type
V.:-.-:"'.' ..-.y.-
Surface Water
P&N 3
1 or
Total PFOS l Microlayer Water
0 0)
Total PF i
3 0)
Bulk Water
Total PFOS l
3x a)
Sediment P&N
&
0 1ST
Total PFOS M ____ Drinking Water (influent)
0
(3)
P&N l Total PFOS 1
1 0) 0 0)
Drinking Water (outflow)
P&N i Total PFOS i
1 0 0 0)
Tap Water
P&N 3
1 0)
Total PFOS 1
0 0)
Landfill Leachate
P&N 1
0 (3)
Total PFOS (1)
0 i3)
POTW (influent)
P&N 1
0 (1)
Total PFOS l
0 (1)
POTW sludge
P&N 1 --------------- u
T3)
Total PFOS (1)
0 (3)
POTW (effluent)
P&1?-p
(!) (i)
-; 'Spike? . P Field SpikeCoat. ^Field,- .Lab..:v:;;i :::laukV-
1 1 1 lOOng/L
00
1
00
I
3 0 0 100 ng/L
(3 (1) 1 0 0) 1
1 1 1 tOOng/L
00
0
11 00
0 0
100 ng/L
1 1 11
00
10 (1) 1 ns 0 ) 0
0 1o
0
____ l_2_____ 0
0 (1) 1 0 (1) 0
1 fT fl
100ng/L lOOOng/'L
O C O -599
n u u c*t u u lUL
u( < i
Quality Assurance Project Plan
Amendment 2 to Reunion No. /
Mufti-City Study Sampling Task (N10Q672)
Effective Date: July 20, 1999
Total PFOS Urban Air
[
f(I)
0
0
!
Page 5 o f \7 "" "
P&N 1
0
0
10
1 filter: 100 ng PFOS
& POAA
PUF: 100 ng Et- &
-Me-FOSE-OH
Total PFOS 0
0
0
00
0
Food (including finfish)
P& N ] 3s Total PFOS (3)"
0 0
(Da 0 (1) 0 (l)4 0 (1) 0
SNumbers in parentheses indicate samples that will be created from splits or aliquots in the laboratory.
`'Triplicate analysis of one sediment sample. `Organic flour at one site, only.
^Duplicate analysis of the fish samples.
P - pula: PFOS, POAA, PFOSA, PFOSAA, M556; N= neutrals: Et/Me of FOSE-OH, FOSE-C1, FOSE-Amide
Table 6 is amended below to include SOPs for finfish and microlayor sampling. Table 6. Standard Operating Procedures
Collection and Handling of Air Samples for die Multi-Cities Project Collection and Handling of Water Samples for the Multi-Cities Project Spiking Field Samples for the Multi-Cities Project Assigning Sample IDs for the Multi-Cities Project Collection and Handling of Sediment Samples for the Multi-Cities Project CPS Operation and Calibration (draft) Sample Shipping for the Multi-Cities Project Sample Custody Collection and Handling of Aquatic Surface Microlayer Samples Collection and Handling of Fish Samples Using a Backpack Electroshocker
s o p '.No/.'
M3-F-1 M3-F-2 M3-F-4 M3-F-6 "Mj -F-7 Battelle 3-164 Battelle 5-210 Battelle 6-010 MSL-T-022-00 MSL-T-023-00
Section 7.3.1 is amended ax follows: Scientific collecting permits and fishing licenses (if required) will be obtained for each state where fish will be collected.
Section 7.3.3 and 11.1.3 are amended as follows: The Battelle Marine Sciences Laboratory and Pacific Northwest National Laboratory (MSL/PNNL) sampling team will be using a Trimble Geocxplorer hand-held GPS to determine the exact location (coordinates) at each sample collection location, rather titan a Garmin 12 XL system. The Trimble unit w ill be operated according to SOP No. 3-164. Deviations from tins SOP will be documented in the field and revisions to the QAPP will be submitted after the field effort.
Section 7.3.5 is amended as follows: Microlayer and associated bulk water samples will be collected at each site where finfish are collected. Both sample types will be collected from one location within the matched sediment/water/fish site for each city. Samples will be collected as described for the whole (bulk) water collected in association with the surface
000500
t i U V W~T V V I V U * T w
Quality Assurance Project Plan
Amendment 2 to ftevision No.: l
Multi City Study Sampling Task (N100672)
Effective Date: July 20. 999
Page 6 o f 17
water source. Two liters of the surface microlayer (MSL-T-022-00) and 2 L o f bulk water
(SOP M3-F-2.0) will be collected into ESS (sic ESE) certified clean bottles.
Section 7.3.10 is amended as follows: Finfish species will include largemouth bass and smallxnouth bass, in addition to perch and bluegili. The common name "bluegill" is used here to refer to any of a number o f different species belonging to the sunfish family (Centrarchidae), including pumpkinseed, redear sunfish, warmouth, green sunfish, white crappie, and black crappie. The [note] in the May 14,1999 QAPP no longer applies.
Section 8 is amended as follows: Finfish, microlayer, and bulk water sampling arc scheduled to take place in Mobile, AT., Pensacola, FL, Port St. Lucie, FL in July 1999, and in Columbus, GA, Cleveland, TN, Decatur, AL in August 1999.
Section 9.1 is amended as follows: Mr. Dave Shreffler from Battelle Marine Sciences Laboratory is die Sampling Team Leader for the fish and microlayer sampling. Mr. Shreffler will be responsible for all sampling activities, including mobilization, demobilization, and reporting. Mr. Shreffler will also be responsible for identifying problems and corrective actions while in the field, and communicating these activities to the Duxbury QA officer, and the Project Manager, as appropriate.
Table 8 is amended below to include Mr. Dave Shreffler in place o f Dr. Dennis Dauble as the finfish sampler and microlayer sampler.
Table 8. Key Personnel, Addresses, aud Telephone Numbers
. Naihe : Marcia Nishioka Program Manager
Don Ounster Sampling Task Leader
Rosanna Buhl Sampling QA Officer
Wayne Trulli Duxbury Field Operations Manager
Michael Mcara Duxbury Sample Custodian
Scon Mscomber Sampling Team leader
Chris Gagnon Sampling Team Leader
Kim Andrews Multimedia Che/ul.vt
' Address/
Battelle Columbus SOS King Avenue Room 5-2*28 Columbus, OH 43201 Battelle Duxbury 397 Washington Street Duxbury, MA 02332 Battelle Duxbury . 397 Washington Street Duxbury. MA 02332 Battelle Duxbury 397 Washington Street Duxbury, MA 02332 Battelle uxbury 397 Washington Street Duxbury, MA 02332 Battelle Duxbury 397 Washington Street Duxbury, MA 02532
Battelle Duxbury 397 Washington Street Duxbury, MA 02332 Battelle Columbus SOSKing Avenue
; Phone . 614*424-4964 (work)
781-952*5378 (work)
781-952-5309 (work)
781-952*5380 (work) 508-888-6652 (home) 781-952-5270 (work)
781-952-5379 (work)
781-952*5325 (work)
614-424-5254 (work) 614-875-3344 (home)
000501
l|U\i UT W *w*- * ">' v "
Quality Assuranca Project Plan
Amendment 2 to Revision No.; /
M ulti-City Study Sampling Task (N100072)
Effective Date: July 2 0 .1999
_____________________________ __ ______________________ _
Fag* 7 q f 17
Room 11-1-060
Columbus, OH 43201
T ony Wifibith Air Chemist
BaneUe Columbus 505 King Avenue
"614-424-5481 (work) 740-587-7439 (home)
Room 6-1-31
Columbus, OH 43201
Dave Shrcfiler
Battello Marine Sciences tab. "360-681-3660 (work)
Finfish Sampler. Microlayer
1S29 W. Sequim Bay Road
Sampler
Sequim, WA 98382
Section 11.1.2 is amended as follows: All microlayer samples will be shipped via overnight delivery to Ms. Kim Andrews at the address shown in Table 8. All fish samples will be shipped via overnight delivery to Mr. Michael Meara at the address shown in Table 8.
Section 11.1.4 is amended as follows: Two labels will be provided for each finfish sample collected. One label is placed on the aluminum foil. The second label is placed between the double Ziploc bags. The labels provide a narrative description of the sample, as well as a unique number that is documented on die Finfish Data Sheet. One sample label will be affixed to each microlayer bottle and bulk water bottle.
Table 9 is amended below to include largemouth and smallmouth bass in addition to bluegill and perch.
Table 9. Sample Containers, Storage Conditions, and Shipping
Sample Source .and Type...
Particulate phase Vapor phase
Surface-water source Plant intake Treated water from plant Tap water from retail
establishment Sediment from surface-water source
potW Post-chlorination influent Sludge Gffluent
Leachate
. Sample Containers
Pre^rvaton and Strage Conditions .....
Urban Air
Glass fiber filter
Place filter in rip-lock bag, seal. Store cool (4C)
Polyurethane foam Wrap cartridge in precleaned foil. Place wrapped
sorbent
cartridge in zip-lock bag and wrap in bubblewrap.
Store cool (4" C)
Drinking Water
1-L precleaned
No preservation. Store cool (4C)
(certified)
polyethylene jars
200ml predeaned
(certified) polyethylene jars
Store cool (4 C)
1-L precleaned
(certified) polyethylenejars
Store cool (4 C)
Landfill | { store cool (4* C)
Local Food -
000502
Quality Assurance Project Plan Multi-City Study Sampling Task (NI00672}
Ground beef Pork chops (heme in) Boneless, skinless chicken
breasts Porlsand-beefhot dogs Regional fish Eggs Whole milk White bread Frozen green beans Fresh apples Organic bread or flour
Finfish (bluegiil, yellow perch, largemouth bass, smalhnoutb bass) (electroshock)
Keep each item in its original container, and keep items separated in storespecific coolers.
Fish Methanol rinsed aluminum foil; plastic bags.
Store cool (4 C) Store cool (4 C)
Amendment 2 to Revision No.: I Effective Date: July 20, 999
Page 8 o f 17
Section 11.8.1 is amended as follows: The "target species" will include largemouth and wnallmouth bass. Elcctroshocking will be conducted according to MSL-T-023-00. Fiufish will be collected from the each site designated as the matched water/sediment/fish site. Finfish will not be collected from the sites designated at "quiet" water bodies.
Section 11.8.1.1 is amended as follows: Individual fish will be placed in methanol rinsed aluminum foil and then double bagged in Ziploc bags and sealed with tape. A label with the city , sample site identification, matrix, date and initials o f sampler, sampling date and time, unique sample ID, and project number will be placed between die double bags and on the aluminum foil. Fish will be placed on ice in a cooler for shipment on the day of sampling or stored on ice overnight for shipment as early as possible on the day following sampling. Fresh ice will be placed in the cooler prior to shipping.
Section 11.8.1.2 is amended as follows: Fish length will not be recorded to the nearest mm in the field. Fish will be.weighed only to determine if the mass is adequate for analysis (i.c., 200 g minimum), not weighed to the nearest g.
A new section is added: Section 11.9 W ater Sampling Associated with Finfish Collection
11.9.1 Microlayer sampling: Microlayer sampling will be performed according to SOP-T022-00, and is summarized below.
1. Locale the water source and a point of access. 2. Record site information, as appropriate, on field log forms or a field log book (e.g., weather
conditions, site location by GPS, observations o f the water body and visible surface accumulations, adjacent site features that could be of significance) on the Aquatic Microlayer Sampling Data Sheet 3. Set up the microlayer sampler frame at a stable and level location. Install wiper and funnel. 4. Rinse equipment by collecting microlayer sample (approximately 20 repeated dips with the glass plate) and allowing it to drain through funnel without collecting in a sample bottle.
000503
Quality Assurance Project Plan Multi-City Study Sampling Task (N)00672)
Amendment 2 to Revision No.: I Effective. Date: July 20, 1999
Page 9 o f 17
5. To collect aquatic microlayer, dip the glass plate vertically into the water gently, with minimal
horizontal movement, and withdraw it slowly. Push the glass plate through the wiper and
allow liquid to drain through the funnel. The sampler frame will require support with one
hand.
6. Record sampling start time and initials of sampler.
7. Start collection of microlayer sample by removing the sample bottle cap and placing the bottle
under the funnel in a position to catch liquid. Continue to dip the glass plate into the water to
be sampled and push the plate through the sampler wiper.
8. Repeat until the sample bottle is tilled. Minimal air space is allowed.
9. Recap the sample bottle securely and check that sample bottle is labeled properly with sample
time and sampler identification.
10. Check with project-specific work plan for guidance for storage and shipment of samples.
Samples are held in an insulated container (e.g., cooler) with ice or under refrigeration at
approximately 4C. Packing for shipment requires use of bubble wrap or other material to
prevent physical damage to and breakage o f sample containers and use o f a sturdy, insulated
container.
11. Complete the chain-of-custody form (see SOP MSL-A-002 and other project-specific
guidance) and attach to the inside of die lid o f each shipped cooler/containcr.
11.9.2 Bulk water sampling: Bulk water sampling associated with fish collection will take place according to SOP M3-F-2.0, and as summarized below.
1. Locate the water source and a point o f access. 2. Complete the Station Log and take a GPS reading (SOP 3-164) as close as possible to the
sampling location. 3. Wipe the exterior of the sampling bottle with methanol. 4. Submerge the sample bottle below the water surface and unscrew the bottle cap.
5. Fill the water bottle by turning the bottle parallel to the water surface and slowing rotating so that the mouth of the bottle is up-right. This procedure will ensure that water from the surface microlayer is not pulled into the sample bottle.
6. Recap the sample bottle under water. 7. Wipe the bottle with methanol and affix a sample label. 8. Place the sample in a cooler o f ice. 9. Complete the chain-of-custody form.
13.2 The Trimble GPS used for finfish and microlayer/bulk water position determinations is calibrated as described for the Gannin GPS (SOP No. 3-164-01).
Section 19.4 no longer applies and should be deleted.
Section 19.5 Backpack Electroshockcr: Maintenance of the backpack electroshocker is performed by the manufacturer.
Section 19.6 Microlaycr Sampler. The microlayer sampler will be rinsed with freshwater, rinsed with deionized water, and finally rinsed with methanol between sampling locations.
000504
Quality Assurance Project Plan
Amendment 2 to Revision No.: 1
Multi-City SiudvSampling Task (Ni00672)
Effective Date: July 20, 999
Page 10 o f \2
No soap will be used on the sampler. The sampler will then be placed in a clean plastic bag
until next use.
Appendix C Sampling Forms. The Finfish Data Sheet has been revised below to more accurately reflect die field data we will be recording. New sampling forms are also included
below for Aquatic Microlayer Sampling and Subsurface Bulk Water Sampling.
00050
nuu
l UU* 1 *!/%*
u m 1LLLL v u n u u n i
Ovality Assurance Project Pion Multi-CityStudy Sampling Task (N100672)
FINFISH DATA SHEET
Project; N 100666-01
Amendment 2 to Revision No.: i Effective Date: July 20. i 999 Page II o f 17
Date
StarVEnd Time
Collectors
Gear type
Sampling location Photo # DCPS Coordinates
Water Temperature Air Temperature Water Depth ft Visibility
Water Flow
Max/Min Stream Widths
Species
ExternsIAbnormlitiei;
SamplelD
Relinquished B y :_____________________ __________________ Datc_
Received By:
Date
000506
Quality Assurance Project Plan Multi-CityStudy Sampling Task (N100672)
AQUATIC MICROLAYER SAMPLING
Battelle Marine Sciences Laboratory 1529 West Sequim Bay Road Sequim, Washington 98382
Amendment 2 ta Revision No,: l Effective Date: July 20. 999 Page 2 o f Vi
Project Number. Proejct Manager Phone; Fax;
Location;
Date: Bottle Lot Number: Collected by (initials);
31565 Dave Shreffter 360.681.3660 360.681.3681
Mobile, AL matched site
- ""
--..................- - .....-- ........................
Sample Number
recorded by: Sample Description and Analysis (initials)
M&4101 B MC-4102 K MC-4103 H MC-4104 B MC-4105 K MC-4106 H
-Microlayer - Battelle (PFOS duplicate analyses: 500 mL each) Microlayer - 3M (PFOS: 500 mL) Mictolayer - Henion (PFOS; 500 mL) PFOS Field Blank - Battelle (empty bottle) PFOS Field Blank - 3M (empty bottle) PFOS Field 8lank - Henion (empty bottle)
Relinquished by:
ate:
Time:
Ice Included (Y/N)
Received by:
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000507
Quality Assurance Project Plan M ulti-City Study Sampling Task (N100672)
SUBSURFACE BULK WATER SAMPLING
Battelle Marine Sciences Laboratory 1529 W est Sequim Bay Road Sequim, Washington 98382
Amendment 2 to Revision No.: I Effective Date: July 20,1999 Page 13 o f 17
Project Number: Proejct Managen Phone; Fax:
Location;
Date: Bottte Lot Number: Collected by (initials):
31565 Dave Shreffler 360.681.3660 360.681.3681
Mobile, AL m atched site
Sample Number
recorded by: Sam ple Description and Analysts (initiate)
MC-4101 B MC-4102 K MC-4103 H MC-4104 8 M C -4105K MC-4106 H
Bulk water * Battelle (PFOS duplicate analyses: 500 mL each) Bulkwater - 3M (PFOS duplicate analyses: 500 mL each) Bulkwater - Henion (PFOS duplicate analyses: 500 m l each) Polars field spike - Battelle (duplicate analyses: 500 mL each) Polars field spike -3M (duplicate analyses: 500 mL each) Polars field spike - Henion (duplicate analyses: 500 mL each)
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Ice Included (Y/N)
Received by:
Date:
Time:
Condition:
000508
Quality Assurance Project Pian SJulti-City StudySampling Task (N100672)
AQUATIC MICROLAYER SAMPLING
Battette Marine Sciences Laboratory 1529 W est Sequim Bay Road Sequim, Washington 98382
Amendment 2 (o Revision No.: 1 Effective Date: July 20, 1999 Page 14 n f n
Project Number: Proejct Manager. Phone: Fax:
location:
Date: Bottle Lot Number: Collected by (Initials):
31565 Deve Shreffler 360.881.3660 360.681.3681
Pensacola. FL matched site
Sample Number
recorded by: Sample Description and Analysis (initials)
MC-3101 B MC-3102 K MC-3103 H MC-3104 B
MC-3105 K MC-3106 H
.Microlayer - Battette {PFOS duplicate analyses: 500 mL each) Microlayer - 3M (PFOS: 500 mL) " Microlayer - Henton (PFOS; 500 mL) ------ ---- ppQg peJ(j aiank - Battette (empty bottle)
PFOS Field Blank - 3M (empty bottle) ' ' PFOS Field Blank - Henion (empty bottle)
Relinquished by:
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Time:
ice Included Vfiti)
Received by:
Date:
Time1
Condition:
O 0& 5O 9
Quality Assurance Project Plan Mult-C/yStudy Sampling Task (N100672)
SUBSURFACE BULK WATER SAMPLING
Battelle Marine Sciences Laboratory 1529 W est Sequim Bay Road Sequim, Washington 98382
Amendment 2 to Revision No.: / Effective Date: July 20. 1999 Page 15 o f 17
Project Number. Proeja Manager Phone: Fax:
Location:
Date: Bottle Lot Number. Collected by (initials):
31565 Dave Shrcffier 360.681.3660 360.681.3681
Pensacola. FL matched site
Sample Number
recorded by: Sample Description and Analysis (initials)
MC-3107 B MC-31Q8 K MC-3109 H MC-3110 B MC-3111 K MC-3112 H
Bulk water - Battelle (PFOS duplicate analyses: 500 mL each) Bulkwater - 3M (PFOS duplicate analyses: 500 mL each) Bulkwater - Henion (PFOS duplicate analyses: 500 mL each) Polars field spike - Battelle (duplicate analyses: 500 mL each) .Polars field spike -3M (duplicate analyses: 600 mL each) " Polars field spike - Henion (duplicate analyses: 500 mL each)
Relinquished by.
Date:
Time:
ice Included (Y/N)
Received by:
Date:
Time:
Condition:
000510
Quality Assurance Project Plan Muli-City Study Sampling Task (N10Q672)
AQUATIC MICROLAYER SAMPLING
Batteile Marine Sciences Laboratory 1529 W est Sequim B3y Road Sequim, Washington 98382
Amendment 2 to Revision No.: I Effective Date: July 20. J999 Page IGafYl
Project Number: Proejct M anager Phone: Fax:
Location:
Date: Bottle Lot Number: Collected by (initials):
31565 Dave Shreffler 360.681.3660 360.6B1.3681
Port S t Lucie m atched site
Sample Number
recorded by: Sam ple Description and Analysis (initials)
MC-6101 B MC-6102 K MC-6103H MC-6104 B MC-6105 K MC-6106 H
Microlayer Batteile (PFO S duplicate analyses: 500 mL eacn) Microlayer - 3M (PFOS; 500 mL) Microlayer - Henion (PFOS; 500 mL) PFOS Field Blank - Batteile (empty bottle) PFO S Field Blank - 3M (empty bottle) PFOS Reid Blank - Henion (empty bottle)
Relinquished by:
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Time:
Ice included (Y/N)
Received by:
Date:
Time:
Condition:
000511
Quality Assurance Project Plan Multi-City StudySampling Task (NIQ0672)
SUBSURFACE BULK WATER SAMPLING
Battette Marine Sciences Laboratory 1529 W est Sequiro Bay Road Sequim. Washington 96382
Amendment 2 to Revision No.: I Effective Dale: July 20,1999 Page 17 o f 17
Project Number Proejct Manager Phone: Fax:
Location:
Date: Bottle lo t Number Collected by (initials}:
31565 Dave Shreffler 360.681.3680 360.681.3681
Port S t Lucie. FL matched site
Sample Number
recorded by: Sample Description and Analysis (initials}
MCM3107 B MC-6108 K MC-6109 H MC-6110 B MC-6111 K MC-6112H
Bulk water - Battette (PF0S duplicate analyses: 500 mL each) Bulkwater - 3M (PFOS duplicate analyses: 500 mL each) Bulkwater - Henion (PFOS duplicate analyses: 500 mL each) Polars field spike - Battette (duplicate analyses: 500 mL each) Polars field spike -3M (duplicate analyses: 500 m l each) Polars field spike - Henion (duplicate analyses: 500 mL each)
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Time:
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000512
ENVIRONMENTAL MONITORING: PART TW O - BIOSPHERE SAM PLING AND ANALYSIS
A plan to assess potential environm ental exposure to perfluorooctanesulfonate and other fluorochemical substances has been developed by 3M and outside experts. One component of this plan involves characterization of the geographic distribution of fluorochemicals in biotic and abiotic receptors. Two studies are in progress, one focused in the vicinity of the 3M Decatur, Alabam a m anufacturing facility, and the other a much more comprehensive global biosphere monitoring program . The prelim inary results obtained to date have been reported in the 3M Environm ental W hite Paper entitled "Sulfonated Perfluorochem icals in the Environm ent: Sources, Dispersion, Fate and Effects."
T he study in th e D ecatur, A labam a a re a is being designed to u n d erstan d the im pact, if any, of production operations in the local environm ent. Samples of the groundw ater, surface w ater, sediments and fish and bird species will be collected in M ay and June, 2000 for analyses. This d ata will be used to evaluate the environm ental presence of fluorochem icals and to assess the potential of any effects using ecotoxicological test results.
The Biosphere monitoring program was designed in consultation with Dr. John Geisy of M ichigan State U niversity. This plan is being viewed as an iterative process to assess global distribution of fluorochemicals. As results are obtained from the global environm ent, the plan is to concentrate on those areas w here fluorochem icals a re detected in sam ples and focus on additional sampling and analyses in those specific locations.
Initially, samples of tissues and blood plasm a are being collected from archived specimens covering different species and locations. Areas of focus include N orth A m erica (G reat Lakes and coastal m arine locations), the arctic region, and Europe. Species to be studied include lake trout, walleye, salmon, catfish, and brown trout; corm orants, eagles and albatross; mussels and shellfish; m arin e m am m als; and other species. This sam pling plan is in progress and as d a ta is obtained an d rep o rts generated, additional subm issions will be m ade to EPA.
Included in this section are the following documents:
1. LCM SM S Analysis of E xtracts rep o rted in: "Prelim inary R ep o rt Analysis of Perfluorinated Compounds in Environm ental Samples" by P. Jones and K. K annan - 4/7/99
2. Final Laboratory 3M Reports on Analysis of Fluorochemicals in W ild Bird Livers - 4/28/99
3. Screening of PFOS levels in Eagle and Albatross - 5/8/98
000513
