Document EdrLGvDzbRb02G4bbqwDvMVy0
to DT21; 63169,FCLawlofNave Rats CODE DT 21; T-6316.9; FC Levels of Naive Rats CODE DRAFT $1499 DRAFT 5/14/99
33MM M MEEDDIICCAARLLepoDDrEEtPPfAAorRRSTTtMMuEdEyNNTNTo,., TTT-OO6XX31II6CC.O9O;LLDOOTGG2Y1Y SSEERRVVIICCEESS
`FluREoerpl~oIhnr-uteLmifofoeircSSartltua((rodFFyCCDca)N)tLeLoeh:.~vTevJeule-lsl6sy3imi18nn,6Ni.N19a9;aic9ivD8veeTaR2Ra1latt~s
In-Lie In-Life
In-Life
EESnntaddrtDDaDatateet:e: :JJuJuullyyly22888,,,
111999999888
BBaacckkggrroouunndd::
bIInnuraadnneanatttfteoemumnppdtt ittono cddoeenttteerrrmomliinrnaeetstthhieenvssoooluuvrreccdeeioonfflslooowmw-el-lee3vveMellcppoeenfrrfllauucootrroodoiocectttaaanrncyesssutululfdfiooennsaa,tteea ((PPFFOOSS)) bbooddyy
ccbooummrdpprerenehh`feoOenbunsjnsiiedvvceeitnippcvll1aoeannn-twwrTioiottlhhritatnhhtvseeesiffntooivllgollaolotvweweiidpnnoggitneoonbsbtojjieemaccltetisiv3voeeuMssrcaacennosddnotrrfreeassccppotoondnntissaeiimtbbiaiinrlliyatitiseeitssounwwdiawaeissst,ddhaeiesnsiigtghnneeedds::tudy
`hhOCoobouujvsseaiicnnntcggiveeaaMrrea1eada-oioTfsfoottnhhi.enevDcereut.sart'rriAgeennnatdtterddpieieeowtttaaeSrrncyytaisscattaluutsddoiiweeuassrscooenansppppoeeofrrieffnllotuuneootdrraiirsnnnuaaitntdeeayddtidotteiensrstetwccctiootohmrmipnwpioottuhuhnendDdsrsts.uaadtty
cMCMoaooarvrrvdyainCCncaaeatssiMeenagaasdsaailanstlodetnercr.nonaDnatdetreu...cAtJJiniinmdmgreWWdwioroleSlecterteasrics,,aft33mwMoMnaisEEtnnaovvpriipirrnoooginnnmmateenneddtnatswaltiul,,pdieiysssrdraeeimssrpepplocoentnosss.iribbwlleeithffooDrr r.
P`cOOoFbboOjjreSdeccitnistvaeietevi2nne2gi--naTTcnooodndtdcreootneeldramuttcistnieneingiifftrdchcieoornmnettctwaatoimma-iiyinrnneamaattirioeoondnniiottoofarfirfnfyeegeesddatnuiisddyllweeoaaifpddeiiNnn-sggEaittmohopyttllhehees.llooww lleevveellss ooff
PMPPeaeFrdrOffillsSuuooosnrre.ooeooncDcrtita.nanncAeeosnsnudutllrrfofoeolnwnaraamSmtesiiaddiconoatteehttwehhaaatsnnwoooallp-((ypNNoe-ia-EEnrtttdhheiydyesllttaFFurOyOdSySsEdtEui))rdeycccootonnofdrdNuucwc-tiEteettddhhyDaaltrt.CCoMovavaarnnvcceCease as
ObaaMlljtlaesedrsmniisaaotvtne.e..3.DrT. oAinndvreeswtigSaetaectaht wbaasckagprpoouinntdesdesrtuudmyandidrelcitvoerrwPiFthOSDler.veMlasirynCnaalseveas
draOaisbtessjseooscffutipddvpiieflfffi3erer-eednnTtbtoyaaiggtneheveggedrsriotofiuugfepparstseenffttrrhoboemrmebddeaidicffekffreegsrrreeoinnutttnssodooubusrereccreoeusbs.mt.aiIIannnneffdodo.rrlmimvDaaetetriiaoPonnnFnOooannSNttalhhebeevbeevvlfaaserrliiinooduunwssaaivse
adappiepptoosiinsnutteepddplssiettududdbyyyddtiihrreeeccdttoiofrrfewwrieittnhht Objectiv4e - To investigate h
Dr. Mary Case as akemate. breeders is to be obtained. Deanna
Dr. Marc Case as alternate.
e possibilty that PFOS exposure i
Nabbefeld
stemming
was
from
atOtaapibipnnjotetieecnddttivffeeeedee4dds,t,-ueedTxyxopdoipirsnoeuvcrestesoiutriningrraraetatet
oomsor a combination of both. Dr. the possibility that PFOS exposure is
rooms or a combination of both. Dr.
sMMtaeamrrvymCCianasgseefwrwoamass
33gaMMthEEenrnvevdiairprioopnnnoommibenjenteentcdtatalilsvtAAeunsdnaayl1ly4ydt.tiiircceaaclltoLLraa.bboorraattoorryy iiss rreessppoonnssiibbilee ffoor cchheemmiiccaall aannaallyyssiissooffssaammpplleess
gathered in objectives 1-4.
"aTTdhhdeerseststusuiddnyygtteooaeecxxhaaommbijinneecetooibvbjejeeaccrtteiivvaeeva33iliissacbcoeom,mprlpeelpteoetreatsannoddftiishetthhveefaorfocicouuusssosoftfuttdhhiiiessrsrewepipololrtrbt..e gOOennncecreeatddeaadtt.aa
addressing each objective are available, reports of the various studies will be generated.
SSttuuddyy OObbjejeccttiivvee:: a"TTnhhyii.ss cssattunuddyybewwadaessteddceetsseiidggnnieendd uttnootrddeeeattteeerrdmmiSinnpeerawwghuhaeattDppaeewrrlfflleuuyoorrrooaootccsttaaonnfeesstuuhllrffeooennaaittfeefe((rPPeFnFtOOSSa))gelleegvverelolssu,,psiiff paaarcncoqqyvu,uiiircdreeaeddnd bffbyrreootmdmheetttvehhacrrreteieeeoduddsiiainffnffieeurrmneeatnnrlttesaubbtprerepedeledideeSerrprsss.r.awgausIIennobDataadaddiwdniielttediiyo.onn,r,atiisnnffooorfrmmtaahttriieooenn dooifnnfertthehneet rraoogddeeenngttroffueeepedds
provided by the various animal suppliers was obtained.
PPaaggee 110o0f1199 22779900..00000011
Exhibit 2790 |
m3MmcMoNn0z04s2s83i1e6
RAFT $1438 DT21:6316.9: FCLevelsofNaveRatsCODE
-DT ~: T-6316.9; FC Levels of Naive Rats CODE DRAFT 5/14/99
Research Client:
Research Client:
Sponsor:
3M Specialty Chemicals Divison 3M Specialty Chemicals Division
33MM CCeenntteerr BBuuiillddiinngg 223366 SSaaiinntt PPaauull MMNN 5555113333--33222200
Buiking 220.26-02, 3M Center 33MM TTooxxiiccoollooggyy SSeerrvviicceess
Building 220-2E-02, 3M Center
SStt.. PPaauul,l MMNN 5555114444--11000000
SSttuuddyy LLooccaattiioonn::
3M Centr, Building 210.SB-181 33MM SSttrraatteeggiicc AAlltteerrnnaattiivvee TTooxxiiccoollooggyy LLaabboorraattoorryy
3M Center, Building 270-SB-181
SSaaiinntt PPaauull,, MMNN 5555113333--33222200
SSttuuddyy DDiirreeccttoorr::
DiAArlleltceertrnnoaartt:ee SSttuuddyy Director: Summary: Summary:
DDeeaannnnaa NNaabbbbeeffeelldd,, MMSS AAddvvaanncceedd TTooxxiiccoollooggiisstt
3M Modical Dep. Toxicological Services 3M Medical Dept./Toxicological Services 39 Center Buiding 220-2E.07 3M Center Building 220-2E-02
SSaaiinntt PPaauul,l MMNN 5555223333--33222200
Tekphone No. 651-737-1374 Facsimile No: 651.733-1773 Telephone No.: 651-737-1374 Facsimile No.: 651-733-1773 Dr. Mary Case Dr. Mary Case Corporat Scientist Corporate Scientist
33MM MMeeddiiccaall DDeeppt.t.//TTooxxiiccoollooggiiccaall SSeerrvviicceess
3M Center Bulding 220-2502 3M Center Building 220-2E-02 Sain Pal, MN 55233-3220 Saint Paul MN 55233-3220
"TTeelleepphhoonnee NNoo..:: 665511--773333--55118800 FFaaccssiimmiillee NNoo..:: 665511--773333--11777733
TThhee
lliivveerrss
ooff
mmaallee
aanndd
ffeemmaallee
Uunnttrreeaatteedd
SSpprraagguuee
DDaawwlleeyy
rraattssoofftthhrreeee
ddiiffffeerreenntt
aaggee
groups
groups
(68 weeks ol, 10-14 weeks ok and old rtrd breeds (OR), = 9-12 mors kl) frf(r6oo-mm8 twthherreeekee~ddiofiflfdfee,rree1nn0tt-b1br4reeeweddeeeerkrsss((BoBrldreeeaedndederroAAl,d, rBBertreiereeedddeerbrrBBeeaadnneddrBsBr(erOeeReddBeer)rC/C)~)w9we-er1re2eemexxoaanmmtilin~neeoddlfdfo)orr BROS and othr known uorochemal (FC) metabolites. Each breedee was conacted 0 ppPrrFooOvviSiddeeaniindnffoootrrhmmeaarttikioonnnowoonnnttfhhleeuoffreeoeeeddhpperrmooivvciiaddlee(ddFttCoot)thmheeieirtrarrbaaottslsitwwehshi.lileeEaaatct htthhbeeriiereffdaacceiirlliiwttieeass.s.contacted to
LLiivveerrssffrroomm BBrreeeeddeerr
AA aanndd
BBreredeerCCrearattssdccoonnettaaiinrneedd
detectable
detectable
levels
levels
of
of
PFOS
PFOS
(215ppb).
(_>15ppb).
``MwMiatalhlaeegrreaat,t (llisivvleeorrpsseffrr=oomm51bbooBtrthheessdooeuurrrccAeessmaiilnnecc;rresealassoeepddess=iiggnn1ii3ffii3ccaaBnnttrllyy e((ppe==Cdm00.a.00el55e))r).iinnTPPhFFeOOScSoccnoocnneccenentntrtrraaatttiiioooonnfn
with age, (slope = 51 Breeder A male; slope = 133 Breeder C male). The concentration of
PROS itivesfrom femal Breeder Aand Bueeder C rats, bower, dilno increas with aaPggFeeO((Ssloinspeli=v=le0r0.s.o66frBoBrpmreeeefdeedemerralAAefBfeemrmeaeladlee;e;srslAlooppaeend==-B-33r.e.77edBBerrreeCeedrdearetCsCr,
hfoemwaleev)e.r,Tdhied
female). The
lniovet risnocfreBarsee ewdeitrh
livers of Breeder
ale ORB had the highest PROS levels (average= 32 & 115 pp). sigifcanly higher tChamntahleelOeRveBlss fhoaudntdhienhiBgrheeesdtePrFAOmSalleeveOlsR(Bav(earavgeera=ge32=71+45115 1p2pbpp)b,)s.igNnoifoicatnhtlyeFhrCigher
than the levels found in Breeder A male ORBs (average = 145 + 12 ppb). No other FC
metabolites could bedetected i he BreederA orBreedeCrra vers. No PFOSor tber metabolites could be detected in the Breeder A or Breeder C rat livers. No PFOS or other
FFCC mmeetatabboolilitteess ccoouulldd bbee ddeetteecctteedd iinn tthhee lliivveerrssooffBBrereeeddeerr BB rraattss..
reacts Page 2 of 19
22779900..00000022
aM_MNCo28317
3M MN00428317
DRAFT $1499 DDTT2~1h: TT-63-16.69;FF3CCLLe1veveel6lssoof.fNNaai9iv'veeRRaattssCCOODDEE DRAZr
BBrreeeeddeerr AArraattssaarxee ffeedd ffeeeedd AA ssuupppplliieeddbbyy CCoommppaannyy AA ((CCiittyyAA,, SSttaattee AA)).. BBrreeedeerBdBreraatrtss
axe fed feed B suppliedby Company B (City B, Sate B). Breeder C ras ae fed fodC are ted feed
ssuupppplliieedd bbyy
B supplied by Company B
CCoommppaanynCyC ((CCiittyy CC,, SSttaattee
(City B,
CC)).. FFiisshh
State
mmeeaall
iBs)t.hBe rpereidmearrCy irnagtrseadireenftedinffeeeeddCC,
is the primary ingredient in feed C,
{he fth Led ingredient infeed A and not presen in fxd B. the fifth listed ingredient in feed A and not present in feed B.
Fromthesedata, it appears s though PFOS greatly bioaccumulaes in male rats, whi in From these
ffeemmaallee rraattss
data, it
iitt ddooeess
$sao0ppt1o0eaaarslleeassssseetrrhdodeuegggrrheeeeP..FOPPooSssssgiirbbellaeetleeyxxpbplilaoannaacatctiiuoomnnssulffaootrrest~ hiins
dmiaflfeerreantsc,e
difference
warheiltehaint
are that
PFOSisreleasedinthemilkand/or transferedinutero10the pupsinfemales that have PbbtohoFrrrOeeouSaagnnhisddurrnneiuulnerraassreseyedddemmxiucnulrtlettihtippeilloeemn lliiitlttkhtteeaarrnssn..mda/AAollreslsotror,aa,tfsnf.eesmfmeaIartlrleesedprroaaistntsstuummltaeaarytyoemmdototorhretaehtrereefapaidusdihpillsmyyeeiaenxlxcf,cerrmaeenttaeelienPPsgFFrtOehOdaSSitehnatvien through urinary excretion than male mrs. It is postulated that fish meal an ingredient in
Some rodent chow,maycontain FC and thus letdo the PFOS found in untreated rats some rodent chow, may comain FC and thus lead to the PFOS found in untreated rats.
"Thepossiblythat 3Mmaterials sed to con thefeed bagsand tha this is source of The possibility that 3M materSal is used .to coat the feed bags and that this is a source of
PPFFOOSS iiss aallssoo bbeeiinngg ccoonnssiiddeerreedd..
Methods:
Methods:
"TThhiirrttyy SSpprraagguueeDDawawleleyyrraattss(f(fiivvee mmaallee,, ffiivveeffeemmaalleeppeerr aaggee ggrroouupp;; aaggee ggrroouuppss =- 66--83 wweeeekkss oolldd,, 1100--1144 wweeeekkss oolldd aanndd oolldd rreettiirreedd bbrreeeeddeerr ( (OROB)R// ==B99--)1122 mmoonntthhss oolldd)) wweerree oorrddeerreedd ffrroomm eeaacchhoofftthheeffoolllloowwiinngg bbrreeeeddeerrss:: BBrreeeeddeerrAA,, BBrreeeeddeerr BBaanndd BBrreeeeddereCCr.. EEaacchh
breeder wasasked toprovidethenameofthefeed provided 0 therras, nameand breeder was asked to provide
llooccaattiioonnooffffeeeedd bbrreeeeddeerr,, ffeeeedd
the name of the feed provided
iinnggrreeddiieennttlliissttaannddiinnffoorrmmaattiioonn
toontfheeeird
on feed
rpaatcs,kangaimnge.aAnldl
packaging. All
aanniimmaallss rreemmaaiinneedd iinn tthheeiirr sshhiippppiinngg ccoonmtaaiinneerrss bbeettwweeeenn aarrrriivvaallaatt 33MM aanndd eeuutthhaanniizzaattiioonn..
No food or water, other than that provided in th shipping containers, was furnished. No food or water, other than that provided in the shipping containers, was furnished.
Within one hour of arrival at 3M, ats were weighed, grossly examined and euthanized by Within one hour of
CCOO,:. SSeerraaaannddlilivveerr
arrival at 3M, rats were weighed, grossly
wweerreehahravrveesstteedd aannddsseenntt ttooKKririss HHaannsseenn,,
3exMaEmnvinierdonanmdenetuatlhanized
3M Environmental
by
Laborator-y Fuorine Analytical Chemistry Team (FACT), for FC analysis. (A Laboratory - Fluorine Analytical Chemistry Team (FACT), for FC analysis. (A
descripotfitohenanalysismethod canbe foundinAppendix 1,Experimentalsection.) description of the analysis method can be found in Appendix 1, Experimental section.)
Statistical significanceof results was determined using th students T-Test (p = 0.05). Statistical significance of results was determined
TThhee rraattee ooff cchhaannggeeooff PPFFOOSS lleevveellsswwaassaannaallyyzzeedd
ubysinplgotthtiengstuagdeenitns mTo-nTtehsst
by plotting age m months
v(per=su0s.0P5F)O. S
versus PFOS
concentration (ppb) and calculating the siope ofthe fn. concentration (ppb) and calculating the slope of the line.
RReessuulltss::
Laboratory Data
cRRoaanwwcenddtaarttaaat,,ioiinnnsccllcuuaddniinnbggebbfooodduyyndwwieneiiggAhhptptsse,,ndlliiivvxeerr1.wweeRiiegghshtutsls,t,sollifvietvreh/bbeooFddyyC wawneeaiilggyhshitts,rraaittniicoolssudaainnngdd gPPrFaFpOOhSSs
30d 3fulldata concentrations
and a full data
cttaaabbnllee,b,cecafaonnubnbede
ifnouAnpdipenndAipxpe1n. dRixes2ul-tsaoSfuthmemaFrCyRaneaployrsits,
found in Appendix 2 - a Summary Report
pirnecpluadriendbg ygrKarpihss
prepared by Kris
HHaannsseenn..
LnLioivtveeryretssaabmmepeplnleessanwwaeleyrrzeeeddde.eeemDmeeepddemnmdoisonstgtmmoenaenatnihniengogfufuutllcaaonnmddetsthhouufossbajanenacaltlyyizzveeeddsfif1ri,rsst2t... aSSnederra4assoaafmmptphleleesosvheharaavvleel
Snstouutcdyyyepptllbaaenne((nsseaeenabblaaycczkkeggdrr.oouuDnneddpessneeccdttiiinoognn,,oppngg.t.he11)),,ousseetcrraoamPPFFesOOoSSfaaonnbaaljleyycsstiiissvemmsaayy1,
n2otanpedr4foromfetdh.e
not performed.
overall
AAvveerraaggee PPFFOOSS lliivveerr lleevveellss ++ ssttaannddaarrdd ddeevviiaattiioonn ffoorr tthhee vvaarriioouuss aaggee //sseexx// bbrreeeeddeerr ggrroouuppss
PPaaggee33 ooff 1199
22779900..00000033
3MM_MNMONO0A042288331188
inicnclluuddeeddiinn tthhiiss ssttuuddyyaarreeaass ffoolllloowwss::
DT31 DT ~'1;
T6316.9:FCLevels
T-6316.9; FC Levels
of
of
aDNaRv~'AeveFRRaTattss5CC7OO1D4D5EE9
DRAFT 5/14/99
BrBBerreeeededederreAAr66A-6-888wwweeeeeekkk ooollldddFMMeimallaeele BrBBBrerreeeeeeeddddeeeerrrr AAAA161100-0-8--111w444wewweeekeeeeokkkldooollFdddeFMmMeaaamllleaeele BBBBrrrreeeeeeddeeeerrAAAAddOOO1eeRR0Rrr-BBB14MFMweamalealeeelke:old Female
Breeder A ORB Female
446633...600.+%2 4419.00.7727ppppbb 71169006.33.9.6.0.0_+_.122211.19.0..3326373 117649415.859.0:0++219112.22.64.043622
.67.8 + 29.63
BBrreeceddeerr BBalalll ggrroouuppss
<<mmeetthhoodd ddeetteeccttiioonn lmimitit ((1155 ppppbb))
BBBrrreeeeeedddereeCCrr 6C6-6-888 wwweeeeeekkk ooolllddd MMFeaamllaeele~' ~" BBBBrrrreeeeeeeeddddeerreeCCCCrr 6111-008--11w044wewweeekeee-ekkoklodoo1kllFddieMmMF4aeaalmllaeeele BBBBrrrreeeeeeeedddedeerrreCCCCr OOO10RRR-BBB1M4FMawelameleaeelkeold Female
Breeder C ORB Female
60.2: 12.21 ppb 79762330....2020+_%+2112122.2..72225199 ppb 7952..72 +2242..4750 337262575.77..006+%2114211.145520.33477
65.6 + 27.24
TTBhhreereraateteeAodofaefcncrhdhaanBnggeeoroff PPCeFFOOrSaeSltislvrivdeeesrrpcecoecontcnievcreneltnyrt.raatTtiihooennswwlioittphheaaoggfeetihisseddelepipiniccttfoiirnnmFFaiilggeuurrBeerssee11adaennrddA22rafftoosrr iBsiBs r55re11eedwwehheriCilAldrestaathtehnasadtirtfsBfoorr1ref3efe3edmameanraldlCee-B3Brra.ret7esrceedrdseepeserrpceAAtcirtvraiaevttlessyliy.iss.00.T.66h..eTTshlhoeepseslloooppfeetohoefftltihhnee llfiionnerefmfooarrlmemaBalleree.aeandndedrffeAemmaraalltees
Breeder C rats is 133 and -3.7 respectively.
PaPgagee 4doorf1199 22779900..00000044
3MM_MMNNC0O024828331199
Figure
Figur~ 1
D731: T.63169:FCLewesofNiDvReARFatTs3C1O4DmE DT 2h T-6316.9; FC Levels of Nai've Rats L-'ODE DRAFT 5114/99
P7OS LiBvBrrreeCeeodndecerAnrAtrations
PFOS Liver Concentrations
180.0
160.0
oo140.0
Timo 120.0
i ws e- 100.0
wo o 80,0
o 60.0
4400.0
20.0
0.0
Hono) 12
*Age (Weeks)
is omens
A=0.0067
45.5
week = 6.8weeks; 12 ee=k16-s14weeks od. 45. weeks = ORB. * 7 weeks = 6-8 weeks old; 12 weeks = 10-14 weeks old, 45.5 weeks = ORB.
Male Female -- Unear (Female -'-~ Une~ (Male)
Figur2e
Figure
PFOS LivBBerrreeCeoedndecererCntrations
PFOS Liver Concentrations
500.0
14S0,0
ge
2g2e00
g.
Prams A05007
oy Male
tin i Female
~Unear (MaJe)
""-"~"LUinneeaar(F(Feemmalaele))
;
as
Hae How)
45.5
we=6e.8wekeksscd 12weeks = 0-14weeks od. 45.5weeks = ORB
* 7 weeks = 6-8 weeks old; 12 weeks = 10-14 weeks old, 45.5 weeks = ORB.
sTTihhgeneifiiinncccarrneteaasbseeetiiwnneePPnFFOaOlSStcrcooennecceaengntetrraagttriiooounnpsffoo(uupnn=dd 0iin.n0t5thh.e Tlliihvveeerrssinocorffeammsaealleein BBPrrFeeOeedSdeerrlevAAelsrraaittnss mwwaalaess
Breeder C significant
Breeder C
rats was between
rats was
significant between the all three age groups (p
significant between the
10-14 week oid group and = 0.05). The increase in
10-14 week old group and
the ORB group, but PFOS levels in male
the ORB group, but
nnoott bbeettwweeeenn tthhee 66--88 aanndd 1100--1144 wweeeekk oolldd ggrroouuppss.. CCoommppaarriinngg mmaalless ttoo ffeemmaalleess aatt eeaacchh aaggee
Faesorts
Page 5 of 19
22779900..00000055
3SMM_MMNNC0O0442268332200
DDTT2211;; TT:-66331166..99:; FFCCLLeevelvsooeffNNlaaiiD'svRveARRFaatTtss5CC1OO4DD5EE9. DRAFT 5/14/99
ggrrrooomuuppbotthhteeh BBBrrreeeeeedddeeerrr AAA a11n00d--11B44rewweeedeeekkr Cooldddifggfrreooruueppdsssiddgiinffifffeeirrceeaddntlssyiig.gnniiCffiioccmaapnntatllryyiaannngddBttrhheeeedOOeRRrBBA ggrrraotosuupptsso PBBfrrForeeOmeeSddbeelroritvCCherBrrcaarottenssecdaaettnetrecraaAaccthhiaonaandggsteeBlh/ssraeeenxxeddggeirrrdooCuumppa,d,liemfmfaeBalrrleeeedeBBdsreriegreenedAifedicrreaCaCrnstolOOyf.RRtBhBCe orrasamttasspmahberaaindadggsseiiBgggnnrrieioffeuiidpcc.eaanrnttAllAyylrhhaioitggtshhheeetorrr aaRPggeFeesOl/ussSleesxxloivfggerrrotocuueoppnTss-cTeddeniidsdtrtasnntiooaottrnesssiisgtghhnnoaiinffwiinccdaaiinndnttllmTyyaabddlleiifeffBfee|rrreeiindnePrPFFAOOrSSatslleeovvfeelltsshebbseeattwmweeeeeanngetthhgeerottuwwpoo.. bbArreleleedodeterhrses.r.
Results of the T-Tests are shown in Table 1.
TTSaTabAblTleeIS11TICS TTS-T=TEAEsTSStIaSTTtTi-IsCtttwSwioco-a-tltaasiiillegedndiddfiiissctatrrniitbbuuttiioonn,, ppaaiirrede,dp,p =: 00.0055,,
* = statistically significant
[BrBorsedeedrer]
A"AggeeC/SSeexx GGrroouupp CCoommppaarriissoonn
[Male Male
Toran [GOWE 70-1awk |GowkE[6awk |10-Tawk [ORB & 6-8 &
10-14wk
2085 [ors la0r8_|oRs_|&6: wk [a 1o-sawk [ors 10-14wk &ORB
1
FoFeamlale ___[ WaMiawleF/Foemmaallse |
i6-8wk& 6-8 &
10-14wk 6-Swlt& 6-B wk
10-14wk OFIB &
ORB
10-14wk &ORB ORB
&6-Swk & 10-14wk ORB
A
"0.0123 "0.0388 *0.0008
0.1139
0.4702 0.9450
0.1043
*0.0478 "0.0125
C
0.1213 "0.0112 *0.0079 0.8620 0,647A 0.3421 0.2564
0.5374 *0.004~=
CT eiwcompoon Breeder Comparison
rr
0@
1
[MGaa wk [7o 0M1aalwek [ORBEAT[5awk Fe[F1me0m-a1laolweek _[_OR_B _Tsooocoonooooacoocmooo|x
55 6-8 wk i e1ls01-104.w1akw0ROE_R|aB& 6-685wkwi |1a01-104-w1k4mfOoRrBs &
A & 6-8 wk,I & 10-14wk ORB & 6-8 wk & 10-14w~ ORB
vs,
C
0.0997 0.4305 *0.0232 0.5157 0.7749 0.9004
BBrreeeeddeerrFFeeeedd//DDiieettHHiissttooryr_y
BBrreeeeddeerr AA rraatss aarree ffeedd ffeeeedd AA ssuupppplliieeddbbyy ccomopmanypAA(ac(cinittyyAyA,, sstiattee AA)).. FFeeeedd AA'iss i`pnpagacrckekadaiggeeenddtsii,nn appaapplieesrrtebbdbaaggysscccoooaamtteepddawwniAitty,hh aaapepaaappseerrfoccloolaaottwiisnn:gg mmaatteerriiaall pprroodduucceedd bbyy 33MM.. TThhee ffeeeedd
ingredients, as listed by company A, are as follows:
ggrroouunndd yyeellllooww ccoormn,, wwhheeaatt mmiiddddlliinnggss,, ssooyybbeeaannmmeeaa,l, aanniimmaall faatt pprreesseerrvveedd wwiitthh
BBHHAA,, f~isshh mmeeaall,, aallffaallffaa mmeeaall,, ccaannee mmoollaasssseess,, ccaallcciittumm ccaarrbboonnaattee,, ssaalltt,, ccyyaannooccoobbaallaarmoiinn ((ssoouurrccee ooff vviittaammiinn BB--1122)),, bbiioottiinn,, DDLL mmeetthhiioonniinnee,, ccaallcciiuumm
``ppAaacnnettotoatththeeenn,aatdteei,-,affloopllhiicca aatccoiicddo,,prrhiiebbrooyfflllaaavvciienn,t,accthheool(leescocaualrlccciieffeeorrfoollvi((tssoaomuuirrncceeE)oo,fftvvhiiittaaammmiiinnn, DDm-a-33g))n,,evvsiiittauammmiinn oOAxxiiaddceee,,tasstooedd,iiauulmlm-asslpeelhleeannititoteec,,onnpiihcceoartiiynnliicacaaecctiiaddt,e, pp(yysroriiuddroocxxeiinnoeef hhvyyitddarrmoocichnhllEoor)ri,iddtehe,i,ammmeeinnna,addmiiooagnnneeesium
PPaaggee6soorf11y9
22779900..00000066
M_MNOOA28321
3M MN00428321
OT
DT
21:
21;
6316.9:FCLevels
T-6316.9; FC Levels
ooffNNa~Dv'vReeARRFaaTtss5CC1OO4DD9E9E.
DRAFT 5/14/99
ddiioimdmaeettethh.yylmlpparrniigmmaiidndioinnuoosll
bobixissiuudlleff,iitteceo((psspooeuurrrccseeulooffaftvvei,ittacamombiiannltKKcaaaccrttbiiovvinitatyyt))e,,,ssfiileliirccrooonnu
dioxide, calciom sdicoaxribdoen,actael,cizuimne
ssiouuldlffaaattteee,,, mzziiannnccgooaxxniiodduee.s. oxide, copper sulfate, cobalt carbonate, ferrous carbonate, zinc
BpBarrceekeeaddgeereBdrBriarntapstsaaparererefbefeadgdfsf.eeee1ddt
Bsupplied by iB usunpkpnloiewdnaby
companyB (city B, ctohims piamney, Bho(wceitvyerB,,
steB). wsthaette hBer).
Feed orFeneodt
Bis also Btheissealbsaogs
arafporaeelclckcooaowagsat:eteedddiwnwiptitahhpaaer33bMMagmmsa.taetIertriiiaasll.uTnTkhhneeofwfeeneeddatBBtihniingsrgteriedmideeien, nthstos,,waasesvlleiisrs,tteewddbhbeyytchcoeormmpopraannnyoytBBt.h,aeasrreeebaasasgs
follows:
gsgorroyoubunenaddnccoooirm,n,,cssooomyybbgeehaainnemmnemeaeall,a,lgg,rrcooauulnncdiduoomaattcssa,,rwwbhohneeaaatste,mmididdiddclaliilnncggissu,,malplffhlaolfsfaaphmmaeteaeal.l,, sboryewbeerasn dorili,cdcoyrenasgtl,uitoednizmeedasla,lcta,lcL-iulymsicnae,rbDoLn-amteet,hdiiocnailcnieu,mvpithaomsipnhAa-teg,cetate, cDDba-r-leaacwccitteiuirvvmsaapttdeaerdndietaadonntyihimemenaaaalsltt,ssett,ieeorrrdooiillbzoe(sfsdoloausuvrairlccnt,e,eoLtofh-filvyvaismiittinaanmem,miioDnnnLDDos-n3)mi),,revvathiiittieaoam,nmiiipnnnyerEE,ivdssoiuutxappimppnlleieenmhmyAeedn-nrtaot,,ccerhnttliiaaaotccreiii,nnd,,e, m`cvmaieeltcnaniamauiddmniiBoopnnyeaenssstoooudpdthipiuelumnemmaebtbeinis,stuu,rlliffbciiotateefllcccaiovoumimmnpp,clltaeehrxxibao((mnssaoiontuuerrm,cceeomnooaofnfngvviaiittrtnaaaomtmeuii,snnpoKKyx)r)i,i,ddeoff,oxollfiiineeceraarhccoiyuiddds,,rsobbuiclioofhtatliitonner,,,ide, ccvooitppappmeeirrnssBuulIl~ffaasttuee,p, pzzliiennmcc eooxnxiti,ddeec,a, lcccaaiullcmciiuumcmariiboooddanaitaeet,,e,ccoombbaaalnlttgaccnaarrobbuoosnnaoatxteei.d. e, ferrous sulfate,
BpBarrceekeeaddgeereCdCrirnraaattss"aacrroeemmffeeedrdcffieeaeelddlyCCasscuucppeppplltiiaeebddlebbyy3ccpolomymlppaamaniynnCaCyte((dcciipttyaypCCe,,rssbtaaatgtee"C.C))I..t iFFeeuendekCdCnoiisws n at this
iaptisammcelekiwsawthgeehedtedbhthyienercraooorm"rcponnamoonttymaaeC33.rc,MMiaamrlmaleytaestarecirfciaoeallplltiioaswubsusl:seeed3dipinnltythhleaesmseeibnbaaagtgessd..
pTahpeefrebeadg".iInt gisruenkdnioiwefneneadttCtshis
The feed ingredients in feed C,
as listed by company C., are as follows:
i`figssrhhomumnedaela#l,2, ssyooeyylblboeewaanncmomemae,lal,g,raalolffuaanllffdaawmmheaoelall,,occaootrmsn,gglwluhuteteeanntmmmeiaedaldl,l,gignrrgoosuu,nnBddrewwwhheoorll'eeswwdhrheieaeatdt,,yeast, gsSooroyyubbneeaadnn#oo2iil,y, esslaalllott,w, ddciicocaarlnlcc,iiugumrmoupphnhodosspwphhhaaottleee,, oggarrtoosuu, nnwddhlleiimamteemssttioodnndeel,i,nvvgiistta,amBmiirnnewaanneddr'smmidinnreieerrdaallyeast, pprreemrnisixese.s.
CCoonncclluussiioonnss//FFuuttuurree DDiirreeccttiioonnss:: TTAhaheendlliivvBeerrsseoeodffeBrBrrCeeienedcderreeBBrasrreaaittnssaPappFppOeeaSarrcfofrnreeceeenootffrFFaCtC.i.oLnLiwivvieetrrhssaffgrreoo.mmLmimavlaeelresrrfaarttssomssuufpppeplliimeeddabbrlyaytBesBrreeeeddeerr sisAnuucpparppnelldaiieseBiddnrbgbeyyefdrBBeorrrmeeCse6dd-iee8nrrcwArAeeaehhskaaesvvieenoddPleeFtttdeoOeccStt1aa0bcb-loee1n4llceewevvneeetellrsskaostoifofonPlPdFFwOaOintSShd bbaduguetetc.rtthehLaeesssivieenellgreesvvsfeerlllossimlpgalafihietcemntaaoualul,l,eydssrllraiigetgtshhittrllyeyd sbbinirrgceenereieddfaeiesrcrisasnn..gtlFFyf,ereommimanallP6eeE-r8rOaatSwsselsseiuukvppespprlolciiloeednddctbbeonyyt1BBr0rar-tee1iee4oddnweerrweieCCtkhsaaccaottgluued.aallallynyFdrddeoedccmerrceetraaehsseaeseseissnlldgiiaggtshhaltt,illgiyyth,, taaalylplttphihneooauourglgsdhharnsneootttitrheodugh PdPseiFFggOrnOeiSSfei.cggarrOneetnaaltyetll,yhyiynbbpiiPoootFaahcOceccSsuuimmlsiuuvilelaartttechesoatniincnPmeRmanOltaSrleaertiirasoatnrts,e,wlewwiahthhsiielladeegiiiennn.tffheFeemmromaamlileeltkrrhaaaettnsssdeii/ttdoddraototaree,assni5tsso0faep1trop0re.eaadlrleseissnasssueetrrtehrooutgoh tdtfhheeeemgarppleuueepp.srsaOtiinsnnfaeferehmemyamalploeeorsstehttehhrsaaeittsadhhiisaalvvytheeaabbbtlooPerrFee1O0aancnSlddeisannruurrePrsslFeeeadOdsSemmdutulihtlntirippotlhuleeeghllmiituttitrelekirrnssa..anrAAydn/enooxortcthrthreeeatrrnispopfonoesstrssrihiebabdinilliiianttryycuiitsmseattrhlhoeaattto rafaettmss.ale rats are more readily able to clear PFOS through urinary excretion than are male AaArlletthhuoonuukggnhhowtthhnee,ssooonuuerrccheeysspoootffhPPesFFiOOsSSillteehaaaddtiinnPggFttOooStthihes cllioivvneetrraiccnoonencdceeinnnttrtraahtetiioornnassciehdneotnwitiff(iOiebddjeiincntttihhviiess 2ss)tt.uuddIytyi tarheouugnhkntohwanf,ioshnemehaylpmoathyebsies iasnthiantgrPeFdiOeSnticsocnotnaitaniinnegdPiFnOtSh.e rWahticlheopwo(Oprbejseecnttivie 2t)h,eIt is
thought that fish meal may be an ingredient containing PFOS. While not present in the
PPaaggee 7Toolf1199
22779900..00000077
3IMM_MMNN00O0442288332222
DT
DT
21:
21;
T4316: T-6316.9;
FCLevels
FC Levels
ooffNNaaDviRveeARRaFtaTtss$CC1OO4DD5EE5
DRAFT 5/14/99
BtBhreere5deeirBnBegraerdadtitcecehnhtoolrwwis,,teffdiifsshhomrmeaBelrael eisdtehthreepAprrciihmmoaawrr.yyiAinnggrrneeddiiaeeonnttfllltisshteyeedFd CfsfoocrroBiBnrtereeesenddteeorrf CCtrhraeattaccthhoocwwhoaawnndd utmuhssaeeeyddb5bte~byyinocebgaaircceahhdianianecnindimtmaalnialsdlbtebradreneea[e'dlodyerezrBreidrisfsecoecudurrererrFenCAntctlolcyynhutounewndnde.ter.Arwwnaaayly.y.sIisInnoaafdddthditeitiiFoonCn,,scsaoamnmptlepenlestsoooffftththheeerfafiitsshchmhmeoeawall
may be obtained and analyzed for FC content.
`rAeAnsnuoolttthhseesrrepcpotosisossnii,bbll3ee MssoosuuurrpccpeelioocffsPPpFFaOOpeSSirsicstohtaheteipnpagappteoerrCccooomaapttiiannnggyuusAsee,dditihnne ffmeeaeenddufbbaaagcgsts.u. reAArssossftaaFtteeeedddiinnA.tthheeIt isirsesnnuooltttksknsnoeocwwtninoana,t3tthhMiisststiuimmpepeiliifefs33pMMampmaetraetcreoiraiaatlilniisgs aatlolssoCuousmseedpdiainnnybbaAagg,sstfhfoeorrmFFeaeeneuddfssaBBctauanrneddrCoC.f. FFFeuuerdrtthhAee.rr It iinnffoorrmmaattiioonn oonn ffeeeedd ppaacckkaaggiinngg wwiillll bbee ggaatthheerreedd iinnaa sseeppaarraattee ssttuuddyy..
SSiiggnnaattuurreess:: PPrreeppaarreedd BByy::
ADDdeevaaannnnncaaeNNdaabTbbobexfeifeceloldld,o,gMMisSSt
DDaattee
Advanced Toxicologist
RReevviieewweedd BByy::
DDrr.. MMaarrvy CCaassee: `Corporate Scientist
DDaattee
Corporate Scientist
DDrr.. AAnnddrreeww SSeeaaccaatt
DDaatee
SSeerniioorr RReesseeaarrcchh TToorxiiccoollooggiisstt
PPaaggees 8oroft1o9 22779900..00000088
3SMM_MMNNo0002482382332.3
DDTT221:I: TT-36131669.:9;FFCCLL~veelsvooffeNDNaaRiilvvAeeRFRaaTttss5CC/OO1DD49EE
AAppppeennddiix x11
DRAFT 5/14/99
Breeder
ID
Se~
[Body Age Grp. !Bwoidgy
Liver lbiliovvederrywwwttt/[[PPiRFndOOiSvSiLdLiuiavvleerr CCoo_n|nAecv..el((Gnnrggp//gg[oSortrp/Gpprpbpb.]
wt (g] wt (g) body wt Individual IAve/Grp IStd/Grp
~A AT 1 [F8wes1 [171[ 7] 1 830 T ood T = T 9 |
lnA21 [kAa2
M M
6-8 wks
bsoawws 6-8 wks
[[111177679119..~756 87880..3833000
0.048
oooo 0.046
"~30~=
aa2g))48.~
43.(;
4.07
llaaAAss43
M !M
ll66-o-o88aswwwwekkssss || 1111665561181]..d56
7.0~=
aeoeel6.6(;
0.044
oooo 0.044
42.(~
a30.r39.5
lAAz656 [[MFFF 66[l--co88oowwwmkkssss | 1[117176367836]3..78 88871..8063969)
0.044
00005s9 0.05(;
64501429.0:)9 66.6 Tz19.27
AhhAee87
[[FFFF
[66[--oo88owsww~kmsss | 111|416167e58]s..113
7.13
7a4o7.46
0.042
ooos0.045
62.0
E65.Y65.4
[AAR911IT00 [M|FFF1_0J66e1--a88w4wwkwkksssa|]
1154071].7 312549..7
car]4.91
6.47
0.035
oo)l 0.041
95192.6] 70501.92
3
=
a2All
A12
MM M
lata [u
J1i0o-114awwisk|s 11o0--114awwhsk|s
sers| 324.3
367.9
sss
10.55
126o8d 12.66
0.033
oooo0.034
100.0
19460.89] 140.(;
103.(;
21.33
llAAaa11ts43a [IM1MM 0[-1110100---1141444vWwwhhkkssSs|
ase356.7 3365586.]5
13.10i
13.213365
0.037
00.0s00.037
a967..8 9807..61
I[AA1T5E '[MF [1100--1144wwhkkss| 232665.1.6] 11034.69]5 A1167 F[F [1100-1144wwhkss| 2102068).1 632 10.49
0000....000004432567]
7979220g2..]1]2 72oq 79.9 12.0~
AA[laa11tt87se JJFFFF ii11o0o0--1-111a4a4wwwwiisksk||ss 2211909063945..445 6667...11314,33" 0000....00003030(80;3]
93.9
792:471.6
[220A19
A2.0
F|FF
_|1014vhs| !10-14wks
10-14 wks
2008] 196.4
209.~
R21 [W [ORB | 4062
770.184] 07.7058
ooo0.036 000.003:~
92.2
7016969.6
o 24g
2lAAe2221a2 wvMMmu ffO*OooRrrRBeeB | s[43i04e2362sf..l~~ 1119000..8007s695
0.024
ooo.oo 0.029
111353131.900 145.(; 12.42
llAAna2222.4s3IImMMm JlOOooRRmrBBss
| 633[0s96s2s53r]].."7 11o3905.z.283e4]3
0.027
ooozn 0.025
11156410999
a6A2.5 iAe2r6
![F[MF FoOO[roRRnsBBs
|| 3a43r94628420f.2]: 111162320..77960(:]]
0000....00003032020!
111[1162909..1900 367.8 3 29.63
lhAAee22.os87||FFF J[OOooRRrrBBss || 44aaes81s2322fe..f95 11t14a468s..800e]]09
0.03~
ooooof 0.03"
4s64755s..0.10
[75 =oi rApAa32oe09dbJFFreedefOOroRRrsBBa
| a2az] 113 485.3 424.2
14.96 11.34
oc0.03"
0.027
59)55.5
53.0
I"~OORutBlo=ro-ldnortetuirseeddbirneceadlecurlsations.
[Method detection mit !** Outlier - not used in
Method detection limit
(MDL): PFOS calculations.
(MDL): PFOS
= =
1155ngn/gogorr/ppppgob.
Pagesor19
Page 9 of 19
22779900..00000099
IM_MN0OA428324
3M MN00428324
:
--------
Breeder
DT 21; T-6316.9; FC Levels of Naive Rats CODE
DRAFF 5/14/99
EET AAppppeennddiix1x1
ID Sex Age Grp. Body Liver liver wt/ PFOS Liver Conc. (ng/g or ppb)
wt (g) wt (g) body wt ndividual :;Ave/Grp Std/Grp
T I1T 1 1 11
B1 M 6-8 wks B2 M !6-8 wks B3 M 6-8 wks B4 M 6-8 wks B5 M 6-8 wks
198.4 9.45 201.7 9.34 203.4 8.50 203.9 8.38 204.2 9.17
0.048 0.046 0.042 0.041 0.045
<MDL <MDL <MDL <MDL <MDL
<MDL
B6 F B7 F B8 F B9 F BIO F
6-8 wks 6-8 v/ks 6-8 wks 6-8 wks 6-8 wks
214.3 7.62 208.0! 7.19 212.8! 7.14 209.3 7.3(; 209.C 8.0~
0.036 0,035 0.034 0,035 0,039
<MDL <MDL <MDL <MDL <MDI
<MDL
Bll M B12 M B13 M B14 M B15 M
10-14 wks 10-14 wks 10-14 wks 10-14wks 10-14 wks
300.6 307.9 304.(; 299.8 306.(;
10.6g
12.33 11.26 10.09 11.38
0.036 0.040 0.037 0.03~ 0.037
<MDL <MDL <MDL <MDL <MDL
<MDL
B16 F 10-14 wks 237.7 6.65 B17 F 10-14 wks 241.1 7.20 B18 F 10-14 wks 239.7 7.14 B19 F 10-14 wks 236.3 8.54 B20 F 10-14 wks 233.4 7.33
0.02~ 0.03(: 0.03(: 0.036 0,031
<MDL <MDL <MDL <MDL <MDL
<MDL
B21 IM B22 M B23 M B24 M B25 M
*ORB ORB ORB ORB ORB
470.7 462.8 446.6 458.2 478.9
17.00 14.90 15.55 14.52 11.19
0,036 0.032 0.035 0.032 0,023
<MDL <MDL <MDL <MDL <MDL
<MDL
B26 F B27 F B28 F B29 F B30 F
ORB ORB ORB ORB ORB
337.8 326.2 285.7 286.2 306.9
10.45 10.27
8.54 9.01 11.00
0.031 0,031 0.030 0.031 0,036
<MDL <MDL <MDL <MDL <MDL
<MDL
* ORB = old retired breeders Method detection limit (MDL): PFOS = 15ng/g or ppb
['age 10 of 19
22779900..00001100
3M MN00428325
DT DT
21,
2 I;
TT-66331166.9:;FFCCLeLveevleslsooffNNaDiaRivveAeRFRaT~tts5s1CC1OO4DD9EE9
AAppppeennddiix x11
DRAFT 5/14/99
Breeder C
P ID Sex FAgpe Grp. gBody
wt (g)
feC r T [ew1 e [2002
llccCC2s12
M M
lo66--aa88 wkw~ksss || 2221000820240..124
lIc:cCs;s43
M M
l6s6--a8a8 wwwwkkssss || 111188884773..1585
[7CCC56s [|FMFr [J66o--o88owwwwekkkssss | [211208801391..9262
C7
lCs8
[FFFF
6[l6--eo88aomwwwekkssss || 2111078772925..]959
IICCc91Ni0o FFFr
_loswe 6-8 wks
6-8 W~
|
E2119099E795..59]
C CCC111l232l M[MMM1011lO|01--o411-414awwwwkkkkssss|| s443553a0449..a]36
llCCeo11tt43ss[MM0fi11m0_00l1--i11o4144awwwweekksess| 4s44or5079775.e|.95l1
l[CCoC11116576 JFMF|r [[110100-0-111T44aawwwmakk]|ss 223212114674d1..]84
oj33c11119878 [FF[FFr 11|[11000o--1111aa44wwwwhekkssss|| 2222121166619..11]
[((633c2a12i090[w!|FFir [1l_OJ0oi--mo11te44awwwkkksss| 2[220e446a651v]e..50]
jjCCoo22ze12szIumMM O[f*OooRrrRBseB | 5s[6ae68sr17soz..]28|
CCljoo222243sa IMuM lmOOooRRrrBBss || 45ss225e5576e]..7]0"
jCC[oc222s657[FMFr :[[OOFoRRorrBBss | 2[258288181416]4.~.~=
jjCCoo22ee78ss[|rFFr OOf[ooRRrrBBss || 2222849417]7..45]
jo |r C29 F 030 F
lOOoRRrBBs
|
sas)289.5
338.7
Liver liver wt/ wt (g) body wt
109s1.e68i4 11o900..z221s698i 10990..73323d46 ss98..aa74oo30 1110059042..426]08
10.54
111133770..609757]7 11114247.7..1168775]]
12.75
583949.3~ 1188842..7491)74] 85911.21
8.53
2212822.22d92 r18a.v2e4 11117478.,92991166 111180988...5215]648 1111458506..5865]15 1147.5985
17.58,
000.00054ad8 0000..0000s55s00 oo00.o.00os55i15
0.048
oooo0.04~
0.05~=
o00c49 0.049
0.053
000o..0c00a348s96] 0o00...o00033d085 0000..0000444004 . 0000...00s034990 ocas 0.047
0.035
0000.00033x; 000.033d3
0.033
o00o34 0.03zl 0000..00005s3691g
0.067
o00ss 0.063 o0s.0e5];
0.052
M*eOOtRhRoBBd ==doeotdledctreietotrnirbeerdmeibetre(deeDdreLrss: PFOS = 18nogrp/pgb
Method detection limit (MDL): PFOS = 15ng/g or ppb
i[PnFFdFOiGvSSidLLuiaivvleerr CCoo_n|ncAcv..e/((Gnnrggp//gg[Sootrra/ppGpprpbb.))
ndividual IAve/Grp IStd/Grp
1 wy 1 7
466633a7..]]~[; 60.2 12.21
447a7977.....2759 3 :
67.0
73.0 12.29
489.3
78.8
73.2
a.q56.6 855p0467r..75] 11518169492..9~0)i
86.2
aR!y52.9
71.5
67.272.6 16147.94
117,0
a341475n191d..900 323397190960,.0000 215750965:..050
105.(;
a70371.3 6239.06
29.(]
zn 92.; 22.75 7 3 75.7 24.41~ EE 327.0 BE 115.37 g65.E 27.24
Page ior19 Page 11 of 19 22779900..00001111
3IMM_MMNN0000442288332266
i sa DT 21; T-6316.9; FC Levels of Naive Rats CODE DRAFT 5/14/99
Appendix 2
33MMEEnvnivriroon~mm enetna~llL~ahboorraattoorryy-- FFlluuoorriinneeA_nPa~lly,yttiiccaall CChheemmisisttrryyTTeeaamm
CCoonntatacct:t: KKrriiss HHaannsseenn FFlluuoorriinnee AAnnaallyyttiiccaall CChheemmisisttrryy TTee~amm BBuuiillddiinngg 22--33EE--0099 88-66001188
SSttuuddyy ooff PPFFOOSS lleevveellss iinn NNaaiivvee RRaattss
SSuummmmaarryy rreeppoorrtt
EExxppeerriim meennttaall SSuummmamr~y
IInn oorrddeerr ttoo aasssseessss ="eennddooggeennoouuss~" lleevveellss ooff PPFFOOSS iinn tteesstt ~anni~mmalalss,, tthhee
llaiinvvaeelrryssozofefdnnfioinnreetPtyyROrraaStt.ssTffhrroromemetthdhirrseeteeidndciifftffaeegrreeennstot ssfuurppapptlsliiweererssrwweererereeprqqeuusaaennntittitetaadtitiinvvteellhyyegroup
analyzed for PFOS.
ooffaanniimmaallss rreecceeiivveedd
Three distinct ages of rats were represented in the
ffrroommeeaacchh ssuupppplliieerr:: 66--88 wweeeekkss oolldd,, 1100--1144 wweeeekkss oolldd,,
agnrodup
and
rreettiirreedd bbrreeeeddeerrss ((>> 1144 wweeeekkss)).. TThhee tteesstt aanpiimmaallss,, rreecceeiivveeddbbyytthhee TTooxxiiccoollooggyy
DDeeppaarrttmmeenntt aatt 33MM,, wweerree ssaaccrriiffiicceedd uuppoonn rreecceeiipptt;; ttiissssuuee ss~ammpplleess wweerree
ddeelliivveerreedd ttoo tthhee 33MM EEnnvviirroonnmmeennttaall LLaabb ffoorr eexxttrraaccttiioonn aanndd aannaallyyssiiss bbyy FFAACCTT..
aB =: SuSpupplpilieerr joe rsa ees[BTTE] BreederA jesila fw [ew al|l [ll aWl LEI BreederB
LLocoacattiioonn
City A, State
AA
City B, State
BB
Chow Feed A
# at
66--88
wks
i0
# at 10-14 wks
i0
#>14 wks
I0
. Ratio
`mm~allee::ffeemmaall
e i:I
Feed B
10
10
10
1:1
Breeder C City C, State Feed C
10
10
10
1:1
C C
**33MM iiss//wwaass aa ssuupppplliieerrooffppaappeerr ccooaattiinngg mma~teterriiaall ttoo ccoommppaannyy AA,, ssuupppplliieerr ooff
ffeeeedd AA.. CCuurrrreennttllyy,, iitt iiss nnoott kknnoowwnniiff 33MM ssuupppplliieess mmaatteerriiaall ttoo ccoommppaannyy BB oorr CC,,
ssuupppplliieerrssoofff efeeeddss BB aanndd CC rreessppeeccttiivveellyy..
liquid chromatography-lectrospray tandem mass spectrometry (HPLC- Analytical Summary
LLiivveerr ssaammpplleess wweerree hhoommooggeenniizzeedd aanndd eexxttrraacctteedd uussiinngg aann iioonn--ppaarriinngg
rreeaaggeennt.t.
TThhee
eexxttrraaccttss
wweerree
aannaallyyzzeedd
qquuaanntitittaattiivveellyy
uussiinngg
high-pressure
high-pressure
liquid chromatography-electrospray tandem mass spectrometry (HPLCEESSMMSSMMSS)) aanndd eevvaalluuaatteedd vveerrssuussaann eexxttrraacctteedd ccuurrvvee.. AAnnaalylyttiiccaall ddeettaaiillss aarree
aafvlvauaioilrlaaobbcllheeemiiinncttahhleecffouunlltll arrmeeippnoorartnt..tsTThahenedpprmreeessteeannbcocleeiootrresaabbwssaeesnnccaaeessccooeeffrorotttatahhiineenerreddkkbnbnyoyoiwwinnnnssppeeccttiioonn..
fluorochemical contaminants and metabolites was
P~ge 12of19
22779900..00001122
3M MN00428327
DDTT2211::TT-66331166..99:;FFCCLLeevveelssooffNNaaDtiRveeARRFataTtss5C1COO4DD5E9E
AAppppeennddiixx 22
DRAFT 5/14/99
RReessuullttRssatSSuulimmvemmraas rrfryyom BreedeAr and Breeder C test animals showed
ssmiiagglnneiiffriiaRcctaaasnntttflereinonvemddroosBggrfeeernneooodmuuessrBrllCeeevveceedollenssortoafAfiPPnaeFFndOOdSSsBi..grneLLieiifdviveceearrrnssCtlccoyotllemllseoetccratteeneddiPmffFarrlOoosSmmtsoohhllaoddneewsseattdnggyrrooouutpphooeffr
ggmrraooluueppriiTannthsetthhfleeriovssmettruuBsddoryyfe..teedsetr
C contained significantly more PFOS than any other
animals from BreedeBr did not contain PFOS above
tthhee
lliimmTPiitthFeooOffSlddieevlettereevsccettloisifooitnnnes(t(th11ae55nplplpipmbvb)se).r].ss
from Breeder B did not contain
ofmale rats from Breeder Aand
PFOS above
Breeder C
rrtooheuuggyhholluPyynFcgcooOerrsSrrteelllmeaaavtteleeledds rwwiaiintttsthh,ht6et-hhl8eeivwaaeeggreeesokoofsfftmtohlhaedel,eaacnnroiianmmttaasallisfs.nr.eodmTtThhhBaaertteleiiesdsa,,esllrtiivvAPeeFrrassOnSccda,ollBlwlreeheccitetleededdetrffhrrCeoomm
ootllhdde,, yrroeeuttiinrrgeeeddsbbtrrmeeaeelddeeerrrammtaasll,ee6-rr8aattwssecceooknnsttaaoiildnn,eeddcottnhhteeahhiiinggehhdeetsshttecclooennaccseetnnttPrrFaaOttiiSoo,nn..while the
determT`iThnheeedlliitvvoeercrssonootffattihhneevffeeermmyaaclloeenrrsaaittsssteffnrrtoolmmevBBerrleesoeeddfeePrrFAAOSaa,nnddshBBorrweeieenddeegrrnCoC cwwoeerrrreeelation
wdwieittthheraamggeie.n.ed to contain very consistent levels of PFOS, showing no correlation
identifNNieood ooitnthhteehrrekklnnioovewwrnnsaffllmuupoolrreooscchhaeenmmailiccyaazlledccooinnnttatahmmiisinnsaatnnutdtsyso.orrmmeteatbaobloilitteess wweerree
identified in the liver samples analyzed in this study.
GGrraapphhiiccaall rreessuullttss aanndd aa ffuullll ttaabblleeooffrreessuullttss aarree aattttaacchheedd..
of PFOCCSuurarrnreednnetttllyyh,,ylmmeFettOhhSooEddssaaalrcreoehbboeleiiinnnggsddaeemvvpeellleoosppoeeddfcffohorrowtthheefraaonnmaalleyyassciihssosofuflplpoolwwiellree.vveellss
of PFOS and ethyl-FOSE alcohol in ssmples of chow from each supplier.
EESxaxpmpeperrliiem mepenrntetapalalration-aqueous samples, HPLCESHS: Ion-pairing eeSxxattmrraapccAltteniiaopolnrnyetpeairsaetxitorna-cateqduferooumsassaammpplleesm,aIt~riIx~Fwi.~thMaSn:fIoon-np-apiariirnignrgeagent ((tteettrraabbAuuttnyyalllytsaemmismmoeoxnntiirlaulmcmtedhhfyyrdodrmrooggaeesnn~msspuullleffaamttee~tri((xTTBwBAAit))h) aininnionaa-pappiHHr-i-nccogonnrtertraoogllleleenddt efelnnuvvoiirrrooocnnhmmeemenintcta..ls. TThhOeenceccaattthiieoonniaiccniorrnee-aaTggBeeAnnttpaissreelleiesccttiifvvoeerllymyed,ttaatrrghgeeettssanalsaynnt~ieoonniiiccs ftlruaonrsofcehrreemdicianlst.o a Ononnc-epotlhaer oarngioanni-Tc BsAolvpeanitr (iesthyfolrmaceedt,atteh),e darniaedly,teanids rrtereaccnoonsnsfsettirirttueudtteeddiniintnommeaetthhnaaonnnoo-llpffooolrrarMMSSoraagnn~aanlllyycssiisss.o. lvent (ethyl acetate), dried, and
IHIPPIL~C-FIEn,SSMMHPSSLaaCr,ntddatHnlPPLIa~lCi--qEuESoStMMoSSfM MSeSx::tFrFaocortrddiesettaiainijlleeecddtedqquuaaalnidltitaaptatisiv~seedwwootrrhkkrough a
reverseIn-phHaPseLCli,quaind cahlriqoumoatt oogfraepxhtircacctolisuminn.jecBteadsedanodn ptahseseafdfitnhitryouogfhthae
reverse-phase liquid chromatographic co]11mn. Based on the affinity of the
apanhnaaalslyeyt,tee tffhooerr ttahhneealssytttaaettiioionsnaarrreyytappihhnaaessdee fiionnr tthaheeccchaoorllanucmmtenarirrseetlliaacttiivvaeemottoountththeeollfiiqqtuuiiimdde.mmoobbiFiloleer
epxhaamspel,e,thien aansatlyantedairsd rseotlauitnieodn PfoFrOSa mcahyarealcuteterisattic10o.5mmoiunnutteosf. t~Rmete.entFioonr
tetiximmaeemssplbbeee,ttwiwneeeeannstaaanssdttaaarnndddaasrorddlutPPioFFnOOPSSFsOsooSlluumttiio~onny
and elute
and
ttahhtee1aa0nn.5aallmyyttieenueetexxtst.rraaccRtteeedtdenffrtriooomnm
sggyrrsootuuennmd.dwwaatteerr iinn tthhiiss aannaallyyssiiss wweerree mmaattcchheedd ttoo wwiitthhiinn 11%% oonn tthhee HHPPLLCC
system.
Page
Page
11330of01199
22779900..00001133
33MM_MMNN0000442288332288
. AAppppeennddiiDDxxTT222211~; TT-:66331166.99:;FFCCLLeevveells~ooff NNaaDDiivRvReAeARFRFaIatT"ts~55/CC1IOG,41DD5/9E9E9
analyzinFFgoolLalloowwwiidinnegg rHHanPPgLLeCCofssoeerppgaararanatitiicoonnc,,omEEpSSoMuMnSSds,pprrooivnvicilddueedssinaag
rapid and accurate means for Qrauporidochaenmdicaaclsc.uratEelemcteraonssprafyo,r
oaaapnnneainrloyainztziieazndtagtiaiooatnnrwettlieedacctehihvnnreiialqqnyuugeemeiuukoslsfeetddoermppgprraeiimnrmaiaactrruiicrllyoeysmff.ooprroMttuhhoneeldeddscee,utteelinccecttsiliouoadnnrienoofgifommnfoilouzlleoeedrcc,ouucpllhaoaersmsioionibncslsa,y,lsifi.ssraggEegenmnleeeercnrattarlelolldysy,praanyd,
ddoeeptteeerccattteeedddE. aStMreSlaMtiSvelyamddilsd
temperatures. Molecules are ionized, possibly
an additional dimension of certainty
fragmented, and
to compound
iiHddoeewnntetiviffeiiErccaSa,ttMiiioonSnns.M.teSadAAoasfds dsisiinnmapnEElSSyaMMmdSoSd,n,iititoaaonraicclnhhagadrratiamchcteteeernprisrisisiottmiincacroyppfrriiiocmnme,aartriranyyinEtiiSyoonnMtSoiiMsscSossmeetllhpeeecocttuieenodddn.
isiHssmoabbwloolemmevbrbearasr,reddicneeosddntdewwaairdittyhhoifohhnsiiiiggmchhfp--erelynangeemmrrgegoynyntiggstaoasusr..innigqAAustsheeaatoprreertisshmuuellatptrryooiffmiohahniirg,gyhhini--oeEnennSaeeM rrreggSycyMrcecSoaoltlltlehiidsseiioaoinnnossdn,,
ddsemetteeaccltlteeeFrddo.s.receoxnadmaprlye,iofnoirc
fragments unique to the primary ion
PFOS (CF,,SO,) analysis, ion 499 is
are created
selected as
and
the
cachhmaaurraacc(tFtceeororirrisrsetetii~sccpmoppnrrpdiilmiemna,agrrfyyotroiiooPSnnF.O.O,)TTS,hhi(i9Css9~iiFoo~nansSmiiOssu~ff'r)r(aaacggonmmraerleyennststpieesodd,ndiiionninttnoog4oo9ttto9hheeiFrsrSiiOsooe,nnl)ess,csstueu1dcc3hh0aaasasstmh88u0e0
E(~(ccamoocurrhrree(sscoppofoornnrteddhsiienpnsgogentdtooisneCCgcFFo,~tnoSSdOOa,Sr.~)Oy),,~)f,11r88a900g9maae~inmnmtuusu ((C(iCcs~FoF.r~dSrSee0tOs,ep)~co),t,nedaadinnnddagn22dto3300cFaSaanOimn~uu'b),e((CC1u33,sF0F~eSSdO~O,m~)t).uo.
EddiiaffcffheerreennottfiiaatttheeesPPeFFOOsSeScoffnrrdooarmmy characteristic 499 amu pri
mooatftrhhryeearrgimoeccnnoobtmmuspptooiuudsinnfddfdsseerteettncthhtaacetthdemmmaiiinggcdhahltt
cchhaoamnapvvoeebseittthhiueeosnesssdaaammnteedo
cssheeaccrooanncddtaaerrriyysitiioocnn f4frr9aa9ggmm~eemnnuttaapttirioionnmappraayttttieeorrnnnssb..u,t different chemical compositions and
HHPPLLCC ssCyyossltteuemmm:n: :HHeewwleletttt--PPaacckkaarrKddeySSseertrioieensse11B11e00ta00siLLliiqqCuu1iid8d CCchohrlrooummmanattooggrraapphh
Column:
2KXeys1to00nemuBme,ta5sipl Co1p8arctioclluemsnize:
Flow rate:
230X0 mI0i0 nram, 5 pan particle size
SSlS~oooolllvvw veeennnrttattABAe::::
M232e.000t0hmm!a.MtMnl/omlaaimnmmmoonniiuumm aacceettaattee
SSSooolllvvveeennnttt GGBr:raaddieiennt:t: MHoeltdhaant44o900l0%%%1tB0o 99f0o0r%%3 BBmiiinnnu8t8.e.55msimniuntuetess
ReHtouldmat0904%0%B Bfiorn3|mmiinnuuttees
HHRoeoltluddraanttt44o004%%0%BB Bffoorrin|1 1mmmiinniunututeete
IRInnujeenccttiiimooenn:vvoolluummee:: 1113.005 lKxLLminutes
Run time:
13.5 minutes
EEMlileecccrttorromosasppsrrsaayyQuTTaaatnntdrdoeemmTlMMAaPasIsssmaSSpspesecctstrrpooemmcetetrteoermreter mass spectrometer
Mass Lynx 3.1 Micromass Quattro II Mass Lynx 3.1
AsoPf[twmaraes.s
software
spectrometer
mass
spectrometer
CCCooonnele vvloolglittaaaggssee:e:ineorgny:
30.60V 4300-6V0.V
MoCMSodoouleldrice:si:eobnlgoacsk
energy: e4l0ecetVrospray temperatuerlee:ctr1o1s5prCay
negative
negative
SDPDeroesiusomorlaclvrveayatbtliiloooonncn:ktteetmemmppeperreaarttauutru4reer9::e9: 221551005CCC
Primary Ion:
499
Page lar19
Page 14 of 19
22779900..00001144
3MM_MMNN00O024828332299
DT21: DT 21;
T3169: T-6316.9;
FCLevels
FC Levels
of
of
NNaaDtivRveeARRFatTast5s 1CC1OO4DD9E5E
AAppppeennddiixx 22
DRAFT 5114/99
ElDDeacauutgrghohidtceecr:TIoonnss:: ~~ 8Z800s,,p999a9,y, 113300,, 118800
Electrode:
Z-spray
QQuuaalliittAyyllccaoonnnattlrryoosllessSuw~emrrmenac~royynducted All analyses were conducted
wwiitthh
a a
mmooddeerraatteettoohhiigghh
lleevveellooffqquuaalliittyy
ceecooaanncctthhrrooglgl.rr.oouuDDppuuoppollffiia~ccnnaailttmmeea~mlmI~asstt..rriiEExxxxccsseeppppiitktkeeaasaasnnnnaaoollttyyeessddeeissinnwweettrhrheeee rrcceeoossnnuuddlluuttccsstttteeaaddbbfllfeoeo,,rrrrooeenncceeoovvAaeennrriiiieemmsssa|lwwee~frrrreeoomm
`wwiitthhiinn tthhee aacccceeppttaabbllee rraannggeeooff 8800--112200%%..
`monitoAAr iccnaaslliitbbrrruaamtteiinootnnacclhhdeerccikfkt.ssttQaaunnaddnaatrrdidawwtaaiossnaawnnaaasllyybzzaeesddeeedvvoeernryyl55i-n-1e10a0rssraaemmgpprlleseesssitotoon
mPaannoFaanOlliyyStssoiiwrssaoiosfnftsbttwawrso~oeidmcceuuonrrntvvaeetlsshdebbrrrriaaRecc.skkpeeoQttnuiisanneggno~efeaaa3tcc-ihh4oggndrrawoouuuagppshotobffeasrssaaeimmdoppnlloseoenssfl..tihnQQeeuuaaarpnnrrtteiiigmttraaaerttsyiisooiinnoonnoo.ff
PFOS was based on the response of 3-4 daughter ions of the primary ion.
RReessuullt`tsSsee spreadsheet attached to this report. See spreadsheet attached to this report.
FPAACCTT KmmeemmHbbaeenrrsssenppaarrttiicciippaattiinngg:: LLK...CCHllaeenmmseeennn HMH... JJEoollhhennfssiooonnn GGRM...WLLE~yalnlnngegnefeesnnobbntutrrgg IRL..bA_WSSymmniinttehh SS.. HHeeiimmddaahhll
Page 15019
Page 15 of 19
22779900..00001155
33MM_MMNN0000442288333300
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[DDTT2~1.:1; T6316: T-6316.9: FFCCLLeevveelsliisnnNNaai~vve~RRataisse-FFurutrthheerr IanvveessttiggaaitioonnooffRRaDattRCCAhhFooTwwC5C7OO1DD4E9E.9. DRAFF 5114/99
"
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SERVICES
SERVICES
fonolRat
-_ Fluoroc.hen~c~l (FC) Levels in Naive Rats - Further Investigation of Rat Chow
BBInaacackknggarrtootuuennmdpdt:: to determine the sourceoflow-level perluoroocnesullonate (PFOS) body
cbbInouumrradpdnreenenahttffeeoonmuusnnpiddtvetiinonpccdlooaennnttetwrrrimooltlrihnaratettshsteihinenfvovsoloollluovvrweeciddengiionnofssblojoomewmceet-.ile33vveMMselaccpnooendnrttfrrrleaausccopttornoddsioiieecbttitaaalrrniyyteisssetutsuulddfwoiiaeensssa,,tdeaaes(iPgFnOedS:) body
compreh`OheOobnbujsjseiiecvncteigtvipeavl|raee1na--woTTfiooththiintenhvvceeeussfrttoriigleglaanotttweedipnipocgottteaeonrnbtytjiiesaactllutissdvooieeuussrrccaoeennssdoporfefecsccfpoolounnnottrasaiimmbniiainlntiateaitdteiisotoennwstwwaicsittodhhmieinpnsiogtthuhneeneddsss:ttuuaddtyy
hCCMooaouvrvsavainnnCcgcaeesaMMreeaadaadsiosiasfolontinh.e.emaDDctruer...rrAAenJnnidtdmrrdeeWiweowtlSaStreeyearacsscta,uattd3wiwMeasassEonaanvppippproeooirinfnnmltuteeeonddrtiassnlttau,utdediydys
dteirstecctoomr wpiotuhnDdrs. at dreirsepcotnosribwleithfoDr r.
ObccMjooeoaocrrrydtdiiiCnnvaeaattsii2enngga-sTaaanonlddtdeeccrtonoennardtdemuu.iccnttJeiiinnmiggfWddciiorroneektccetatrmaasii,inr3ammMtoiononiEnitntooovrfrifiirnneoggendmaainnesddnltewwaaildi,ppiieensgssraaemmsopptplohelneses.sl.ibolwelfeovrels of
OPPPeFFbrOOjleSSucotissrveeoeeeonnc2tii-nannTcceooosnnudttlrerfooteollrnmaraatmtissnideiinonifttehchteeohnattnwtwaoooml--yi(yneNeaa-atrEritoddhniiyeeolttafaFrrfyOeyeSssdtEtuu)idsdycyleoooanffddiNNun-cg-EtEtettohdhyytalhlteCloovwanlecveels of
PMMaeadrdiflsiusooonrn.o.ocDDtrar..neAAsnnudd[rfroeenwwamSSeeiadaccoaatet twwhaaasnsoaalpp(ppNooi-iEnnttteheddyslstFtuuOddySydEdi)rirececoctnotordrucwwtiiettdhh
aDrt .CMovaarnvcCe ase
Dr. Mary Case
as
as
aalltieermnaattee. Objecti3v.e
To
investigate
the
background
serum
andliver
PFOS
levels
in
nave
rrdOaiabetss_tsiooecfsftuiddvpiipefflff3ieeerr-edenTnbttoyaatigghneeevdeggisrrftooifugueppartsseenffttrrvhooeemmnbdddaoiicffrkffseegirrreseonnuttonssbdooueusrreoccrbeuestsm.a.iIInannenffddoo.rrlmimvDaaeettraiioPonnnFnOaoonnSNattlhbhebeeveevvflaaserrliiindoouunwssaa',s've
daaOppiebpptoosjiisnneuttcepeddptl&ssii-ettuduvTddboeyyydditiihnrrveeeeccdstttioofirrfgeawwrtieeittnhhtt hDvDrer.pn.odsMMosairabrsriyylisiCCttayoasstebehaeaatossPbFaatllOattieeSnrrenenadatt.xee..DpeaonniasNstuaebmbrmeifeenlgd
was
from
Ottaaapbiipn_notiteeiecdndttiffveeeedee4dds,t,-ueeTdxxyoppodoisinsruuverreceetsotiiirng.arrtaaett
trrhooeoompmossssooirrbiaalicctyoommtbhbiaintnaPattFiiooOnnSooeffxbbpoootthsh.u. reDDrir.s.
sMteamrvmCinagsefrwoams
Mary Case was
3M EnviarpopnominentetdalstAundaylydtiirceacltoLra.boratory i responsible for chemical analysisofsamples
g"3gTaMahttehhEeefrroneecvdduisriioonnnfoomtbbhjejieensccttatriilvevApeeossnrat11l--ys44t.ticoacloLvaebrotrhaetodryatias troedspaotnesicbxlaenfiorrincghtehmeicpaolssainbailylstiysooffrsaatmcphloesw
Tccreoohpnneottraaftmmo.icinunOasatntiocioofennt.hf. uisrTTthrhheeeeprsosdttruauttddiaysyatttodoodcreeoexxsvaasmemiriinnntgheeeeooadbbcjajheetcacottbtiijovveeecdt33aiitviessececaoxormamepmlapievnltaeiinetelgabanltnhedd,eiircpseoocpsvosoievrbertilreioetfddytoiiennfa8rsvaseaterpcpiahoarouraaswttee
rssettuupddoiireetss. wwOiillnllcbbeeefgugeerntnheererarattdeedad.t.a addressing each objective are available, reports of the various
-- "The 3M Environmental Laborator--y Fluorine Analytical Chermistry Team (FACT) i
TccAuuthrrterraeec3nnhMttellydyEiiinnsnvattihhrpeeornpepmrlriooemccnieetnssaasslroLyoffasabaunnomaarlmalyyatzozriirynnyggr-errpaaFottlrucctohhgrooiewnwneessrAaaammntpaeplldlyeetbssiycffaoo3lrrCMPPhFEFenOOmvSSiisrtaaornnynddmTeiietnssatmammleet(LtaFaabAboboloCilritIate'e)tsso.i.sry in
AA`LAautubtggaouurcsahsttteodoorffiise11sa9999ap88nrde((lAAiNmppTpipHneeannwrddyeiirsxxeum11a))n..malaSSyraayzmmeprdpellpefeoossrroot2ff-g(rreaaNntt-ecccrtahhhtooeywldwpbeffrryrofom3lmMuoCCroEoovvonacavninicraconeeneLLsmauaeblbnofortoaarnaltatoLmoriaridibeeoso)s,r,-aeHHttaoharryrlyllaainnn
aCaLlloaccvbooaohhnrooacllteo((ErEcitheGFsoFOwOa.SnSdEET--NhOOiIHHsH))pwrbboyyemrGGepCtCaeMnMdaSlS.yt.hzeeTTd3rrafaMoccreeEssn2ov-o(iffNrEEo-entFFtmhOeOynSSltpEEael--rOfOLluHaHobrwwoFeeiorerceletdaffnGooeurusnonuddulpfiionnntaaoamscsaoailmdmloppel)cl-eeteetoodhffyali,
a`CaEiiornrbvvboiaorrnnmoceenemppecaanhrrtottaiiwclcll.eLe aTaabnhndidbsawwpsiireppodeemosspnaattmmehpdpelletehdssaetooa3nnMtssoiditEae.tn. evPPiarrroeeenlliimmmtehiiannntaatarr"ylyCLccoaoonbnntcacFlmluiuiessnliidaootnnGissoroddnurriapanwwctnnoonbbctyyorlottllheheceatneidmaalisr,
aEmmnanaivyymiarbbloeentddmouueaeenntot1oatlhrrLeeerssappbdiiurrbraaaittsinioegodnnsoooafnfmpaatliihrreebbohdoarrannmtedaelEEtionGtFgFdOaOatnSSedEEa--prOoOesHHsth,i,abffltryro"oCmfmroottnrmrtaaannsmspsffoieenrrraatdooiifofcntteceissonttnctmmoaaatmnteiterrnrioaiaaltlliafnorrniooommmfatlosohnnece
affonooiomddasslootuuorrccaeen""o..ther during sample handling and possibly from sporadic contamination of the
Page
Page
t1 ooft5
22779900..00002200
3IMM_MMNN0000442288333355
DDTT2211::TT-66331166..99:;FFCCLLeveevellssiinnNNaavivee-RRaattss 2=FFuurrtthbce~sIInnvveessttiiggaattiioonnooffRRaaDttRCCAhhFooTwwC5C/OO1DD4E9E9..
:
AAppppeennddiixx 1|
DRAFT 5/14/99
Furi
geal Cb o
3LLFilMisusaoarEDDinniiecvckiA/rkn/oanIKlCrymdit-isisecnHHatalaannClsshLeeenmnaibsotrraytoTreya-mFluorine Analytical Chemistry Team 6FBBuluui1iloJddr2iiinnnegg.A227--n33aEE7ly--/0t08i699c1a.2l -C77h7e58m-4i6s0tr01y8Team dick @mmm.com/ kipansen@micmomm. 612-778-7540 / 612-778-6018
ladick @mmm.com / kjhansen@mmm.com
Preliminary summary report: Further Investigation of Rat Chow Preliminary sununary report:
Further Investigation of Rat Chow
SSuummmmaarIryny::June 1998,severalrat chow samples from Covance Laboratories and 3 from
NaNrTIeHHbewwieneIrgrneemJssouuunnppiepptlloii1ree9ded9di8t1o,nasttheheveeEEnrnanvlvisirrirtaouotdnnicmmehmeseoncntwotaanallsdaLLumaaclbbptleeffdoosrrbfrccyohham3arrMaaCccttToeeovrrxiaiizzncaacotteiliooonLngyaoo.bffofSrAlauutooosrrirooincecghslheeeammsniidcidcaeal3lossfftrtthhoheama:tt
cbaboraaenggtbaemcciihnhnogoawwtmioonssnaaimttmohppralelneedtihnfferroaosmnmaimmpaCCllooesvsvtauafndnrcieeoeesmcwNwoTanaHssd.ucddteeBettedeecrrbammyuiisnn3eeeMddcoTnftootoxaimcbbioenelaongootyfsf. wssAeiiggrnnseiiinffifigccolaauennnttsdlli,yydeaihhoriifggahthnheederr
acaFiiiorernblbtodoarrmGnnreeionupappata.irrottiinccSllteeohaaatnnhnaddtthwwreieippsseuealmsstapacmmloepupsllleefdsrsboemwweerbrNaeesIHcecodo.llloleenBccttemeecoddaruooesnnethsscaiiottneentbbasyyminittgnhhlaeeen33tssMaMmwpEElenenrv,eviiarrfdooodnunimmntedieno,nntataaalillrbLLaaanagbbsd
soFoufifemlccmdhhaGorowywr.ouffprro.ommSoCCotohvvaaatnnrcceeesualatnnsddcoVVueeldnnddbooerrbaBBsedwweeorrnee maaollssroeo taahnnaaanllyyazzseeiddngaalennddsamaarrpeelerr,eeappdoodrrittteeioddnaiinnl bttahhgiisss
s(uEmWmFOaSrIIyEnn.-OHtthh)ee waccshhoowqwuanstsaaimmtapptlleeedss,,by22--G((CNNM--eSett.hhyyllpp~eerrEff(lluFvoOorrSooEoo-ccOttaHannecwssauulslffoonnroaatmmiipddrooe))s--ecenttthhyylalboaavlleccoothhhooell
d(EettFeOciSoEn-lOimHit) owfa1snqgusancthitoawtedin btyhe GtrCipMliSca.te aEntaFlOyzSeEd-sOaHmplwesasfrnoomt tphrreeeseCnotvaabnocveebathgse
d(b(laleagat.bbeeeclltTeeirdodinpmmlelieiacmalalsitsteffooocfrrhJ1Jouuwnnngees/ag99m,,cpJJhluueonnsweehia11nv5,et,ahannenoddttriJJbpuuelnneiceneat22ae1n1,a,alnrryeaezssleyppdezececftdtoiirvvseeaPllmFyyO))pSlooe.rrsffrfrrooommmtththheeresseiinnCgglloeevVVaneecnneddoboarBgrBs
bag.
Kurt
TOlrAAidpieilrrincbaaaunntreddgcaahiiorofrbwbotorhsnmeaeem3pppaMalrerttsiiEcchlnlaeevvisesraaonmmnopmptlelebensseteawwnleearrLneeaablccyoozlFlellideeeclcftdtoeerddGPraaoFttuOpCCS.oo.vvaAaninrcceewabbsyy
JJpiiammssWWeodlotltteherrroaaunngddh
ccKbhhyuaarrJrtccioomOaalWlldaoaennlnddebugrglrlgaaassnssodbffiKbetuehrrretffii3lOetMlerdrsse..EnbnDDuvereitgrtaao.ilnltqsmTooehfnfhettahellearLssgaaaembmstpplFlviiinoneggllduppmrGreoorccoaeeiufddpuu.srraeeAmccpiaalrnenwsbbaeefsrffooopmuuannstdsdheeiidnnbaatahcrrrkeeopspuoorgotrhf.t
rerboaoyocomJhminssr3owwWoeemrroeelwtecceroorlleallelelcccdotteelKddeucootrtnnedOggllloaadnssessncbffhiuiabbrreegcrr.ofafiilTllteeharrenssdlaaannarngddaelaasynntzaaevlldyoy.zlzueemddWeiwwhpahieiilrleessaassmamampmplpplelelsesessffrffrorroomommmathlttlhheeerbafftarrcoobkmnastckooosfff
weaecrhe aronoalmyzwedebruetcwoilpleecstfedroomnccahgaesrc,odaolorahnadndaineasl,yaznedd. flWooripsewesrame pnolet.s from all rat backs
awtelreevealnsIIannlbyaaeziilerrodsswaabmmtuphtplelweepssirpatteacastkkiefecrnnaolmffrqrouocmaamngtRReisoot,aootdmmiooon33r00hl44ia5m5n,i,dtEEle(tFsaF,pOOpaSrnSoEdEx-i-fOlmOoaHoHtreswwlywaasse0r.dde1ee7tnteeopcctptt.ebedd/Lbb)yybHHuPPtLLaCCb_/o./vEEeSSMMthSSe
Pmameteertltfehhlvoooeddrlsoobceiddlaeoenttweeeccsttuthiiloeofnnopnraatclletiimimc(iiatPtlFqOuS((a)asnppwtppiatrrasootxxinoiiomnmtaatldtieemeltlyiyetc(taepd00p..a0r0o22xleimvepaplstppebalby/bLo)0)v.e17thiinpenpbbl/aLssnoo)kmmsbeeuint aanbssyoaamvmrpeoplolteemhss.e.
PAnearlfylusoisrooofcwtainpeessulffroonmatethe(PbFaOckSs)owfrastnsoitndRetoeoctmed30a4t 5levceolnstaaibnoevdeatmheeabsluarnakbslien aanmyournotomof.
AEsEaAtnFmFapOlOlySSisniEEsg--oOOtfHeHcw,,hinpwwieqhhsueeerf,rreeoaawmssiptwwehieipspeabemssapclkffrersosomomwferrraraatttesss niiinonntRooqotutohahmneetrri3frri0ooa4obo5lmmecs.sodndWitiiaddpinenneosotd.t.farmoDDmeuuaaeesnuittrmooaabllvveaabrraaiicmaakttoisiouonnwnssetroeiinn.f
nsnaoomtt aapnnliaanllgyyzzteeeddchffnooirrqPPuFFeO,OSwS..ipe samples were not quantifiable. Wipes from animal backs were
Page20cs
Page 2 of 5
22779900..00002211
3IMM_MMNN0000442288333366
s
DDTT2:1: T6-36136165.:9; FFCCLeLveevellss innNNaativee RRaatss -FFunrhthrer InnvveessitiggaaitioonnooffRRaaDttRCChAhoFowTwC5CO/O1DD4E9E.9.
DRAFT 5/14/99
AAppppeennddiixx 1I
CChhooww ssoouurrccee
--_-- Vendor B
CCCooovvvaaVannncecnceedeo--rJJuJBuunnneee99
CCoovvaanncc11ee55--Jujunnee
21 Covance 21
-
June
EAFOSEOH NNaummEbtbeFerOr ooSffE-OH cusamm3pplelss
3333
3
33
Levels in Rat Chow Matrix Spike Levels in Rat Chow
Matrix Spike
PsaPamapsssle 2s2s//aassaammmmpppllplleeee
2/sample
s2/asammppllee
EEFtFOOSSEE-NoOndOeHHtect NNNNoooonnnn-d--deddeteetetteeecccctttt
Non-detect
NNoonnd-edteetesctt
EEWtFFOOSSEE--OOHH LLeevveellss iinn CCoovvaannccee LLaabb EEnnvviirroonnmmeenntt
CovSama ple Ln ocatc ion e Number of E(FOSE-OH detection Covance Sample Location
Number of
EtFOSE-OH detection
ems RRoooomm33004455AAirir SSaammpplleess
((GGFF aanndd cchhaarrccooaall))
samples 44 oouutt ooff 8
00..0022 ppppbb//LL <<ddeetetct<e<0c0..11t77 p[pb0/L
Room 3004, 349 Air 16 Non-detect `SSaammRppolloeessm((G3G0FF0aa4nn, dd34cc9hhaaArrcciooraall))
16
RRoooomm 33004455 RRaatt BBaacckkss
66
RRoooomm 33000044,, 334499 RRaatt BBaacckkss
1212
Non-detect
DDeetteecctt ((88 ppppbbi/wwviippee aavveerraaggee))
NNoonn--ddeetteecctt
SEEaxxmpppeelrreiipmmreeennptataarllasstuiuonmnum:naMareryyt:h:ylene chloride extraction
aSnadmtphleenA`pcAronnevapaleylartyreeatsdtiwwwoeinert:reheM2ee0exxttahrmyacLlcetettenehddeercffr.hroloommrNioccdnhhe-ooepwwxotrlbbaryyctaoaidrdodgdniaitntiiioocnnanooafflyeettthehesertr.r. anSSsaalmmeprpllfeerssowwmeetrrheee wwceheioiggwhheetddo
tFathihnfeedteootehrrnggeaanmnnciLiccoovlafeaycyreteerhdr..ewr SSiwtaahamms2pp0lrleeemssmLowwveeeertrhdeeearss.nhhdaaNkkbeeolnnno-wffpoonorrlda11or whhonoorguutarronaai1cnn1dda0nttah2hleeymnntLecc.seentnrttarrniifsfufueggreedfdroffoomrr
30the
30
mmcihinnouuwtteestso..
Fifteen
Glass fier mL of ether
Glass tilter
wffiabbseerrremaannoddvedcchhaaanrrdccoobaalllowaanddssdooorrwbbaantteetsso
1wweteorree2
mssppLlli.itt
aanndd
tthheenn
pprreeppaarreedd
bbyy
eeexxxtttrrraaacccttteiidoo.nnFiwwniiatthlh smmaemetpthhlaaennvoollooorrleettwuhheeerrrm..e |eAAmllLlsl .ooff tthhee cchhaarrccooaall iinnssiiddee tthhee sstteerriillee ttuubbeess wwaass
extracted. Final sample volumes were 1 mL.
GGCC:: CChhaararactceteririssttiicc rreetteenntiioonn ttiimmeess chromatIIonngrggaaapsshiccchhrrcooommlauatmtoong,grraapIpnhhdyyi,,viaadunnalaallciiqoquumoopttoonofefsntsaasmmpoplfleethiiess siiannmjjepeccltteeeddadaasnnoddrbvvaatoppootrrhiiezzeesddtatooinnottnooaraay cpphhraosmheoaoftofatgthrhaeepcshociolcluuecmmonlnu..mnAA.sstInthhdeeitveitdemumpaeplrecaroatmtuurpreeoinsiesnrtarsaiisoseefddt,,heccoosmmapmpoonpnelenentastdssaoarrrebeetheoltutteheeddfsftrraootimmontathhreye ccoolluummnn bbaasseedd oonn pphhyyssiiccaall aanndd cchheemmiiccaall cchhaarraaccitreirsisitcics.s. AAnn iinneerrtt ggaassecoouuss mmoobbiillee pphhaassee
ccaarrrriieess tthhee ccoommppoonneennttss tthhrroouugghh tthhee ccoolluummnn.. CCaarrrriieerr ggaass ffllooww rraattee,, ccoolluummnn tteemmppeerraattuurree aanndd ggaass pprreessssuurree aarree aaddjjuusstteedd ttooooppttiimmiizzeecchhrroommaattooggrraapphhiicc sseeppaarraattiioonn..
MMSS:: EElleeccttrroonn IImmppaacctt
electroOnsOn. neeTmnmaedetdhiotditfofnoorth rpoprtroohodeduufccod iirnnmggatiiiooonnnssoffoofarr mmmaoaslsssecssuppleeaccrttrriaaonii,ss abbyysebbriooemmsobbafarrrddemmaecentnittonwwsiittlhheaeednnseertrggoeetttihicce
effoloerrcmmtaraottinioosnn.
ooIfnf
ooattdhhdeeirrtifofrrnaagtgommeetnhntet
ions that may be larger or salle than the molecular on formation of a molecular ion, a series of reactions leads to
ions that may be larger or smaller than the molecular ion
aatnnhdde
aarree uusseeffuull ffoorr ccoommppoouunndd iiddeennttiiffiiccaattiioonn aanndd qquuaanntitittaattiioonn..
PPaaggee33 ooffS5.
22779900..00002222
3MM_MNMONO0A042288335377
DDT22L;; TT-66331166..%9;;FFCCLLeevveell.s~ iinn NNaaiivveeRRaattss Fre - Fmther Ilnvveesstiiggaatiioonn ooff RRaaDttRCChAhoFowTwC5CO/O1DD4EE9..9
AAppppeennddiix x1I
DRAFT 5/14/99
HHPPLLCC:: InCChhHaarParLactCcet,eririsastntiiccalrrieeqtteueonntttiiooonnf tteiimxmteerssact chromatIongrHapPhLiCc,coalnumanl.iquBoatsoefd eoxntrtahcet
aiifssfiininintjfeyeccottfeteddheaannaddnppaaalssysseedfdortththrhroeouusggthhati3aonTlaiqriuydidphappshheaassieen
ctathhhmeeroouccmonotlaulotmoafgrntriarrmpeeehlla.aicttiivvceFeooltturoomettnhxh.aeemllpBiilqqaeuus,iiedddimnmoonoabsbittilhlaeeenppdahahfafraissdneei,t,SyotthlohueeftiatahonnenaalPaylnFtyeaOlyiiSsterrmeefattoaayriinntcehedldeuffseootarrattiaao1ncc0hah.arar5yraacmpctihtenearurisitsesettsiiicn.c
RafRiemebtetoeerunsntnitiintootnnohfittsitaimimnmeeaessl.ybbseeiFttswowwreeeeeernnxeaaammassptttlaecan,nhddeiaandrrddatoPwPstiFFatOnOhdSSianrssdoo1ll%suuototliiuonotnntiohaanennddPHFPtthOhLeeSCaasnmnyaasalltyyyetteeeml.ueextxettrraaactctte1edd0.5ffrromomminffuiitleteesrr.
fibers in this analysis were matched to within 1% on the HPLC system.
EESS//MMSS::FDDoleletoecwttiinnggeaaHncnPddLmtmoCnoiinsteitopnoarrriiagnntggiommno,olleEeccSuu/llMaarSr iioopnnrssovides a rapid and accurate means for
aoannnaaellyyozzftiinnhFggoelaaloswwowfiiiiddneeesgtrraHainonPnggiLeezCaootfifsooeonrprggataarenancitihiccnoinccq,ooumemEsppSoao/uvMunanidSldsas,b,plreiio,nnvcciillsduuedgdsieinnngegaraSfrlluaulopoyrirdooocpchaehenremdamitcieacadcalclsasu.t.rarteEEelllaeetmccittverreoaolnssyppsmrriaafyloy,dr,
otcteehnmmaeprpegoerefraattrthuuarerneegssse,o. ftmeMM/soztolile=oeccnuui1lzl0eea0stsiotaanorreete1iico2ohn1nnii0zizqeeiudds,e, smfforaranvagigamtimloeaernbnetltedee,dd,f,isoaalgnnoeddwnieddnreegattleelycdctitreeoeddpc..et-rafIItlnenoiidtwaialallitlyyn,rj,eelcttahthtieeiovnemmlayoasfssmstihttleodo.
csSsaahemmadprpltgeleoess..rsaenTIcogotnnessimccohnh/azsatrrhaa=cactttee1crr0iais0snttiitccbooeofm1fko2knn1nio0otwwoinsrnemdffllusoueonolrireotococctrhiheevedemmliyifccoafallollrosswqwwuieanernrgteeitdooabibrtsseieecvrretvverefdeldo.s.uwlTsT.ihhneejsesceetirroeenssuuoltsfs
tahree.
are
cuhsaerdacttoerA`siAennslataelilcyyctssiiiossofnoosEfftXhooFarrtOggacaSnanEini-ccbOeffHllumuooiorrsiinnnimeeto/rzssettdaa=nnsdde6aalrer3ddc0ticcvaooemmlmuyp,pfooouucrnonqrdduraeiinsnntpddiotiiacnctaadittvieenessgrteththsoaauttlttstth.hheee mpparrsiimsmaaroryyf itiohonen
c`ccohoCmamUrpFapoc1otuSuennOriddsstNic(Ccoc3oofHmm:Epp)ltl(FeeCOxxHeeSd:dEC-HO.HOttoo)IisCHm_a,c/aCzectOae=Otee.63S0iaannngaiilionoenuni, oncomffrroroonemismtpoornidnttighnheegwatso rruuusthnenendiinn(mgg0udsestebboruufmffiffnteehrer:e.:
tCthhseeFc~coSonOnc~ecNnet(rnCat.t~iroHnaso)to(fCiftHtohhi2nissCiiHoon2niOinnHtt)hh/CeesHsaa3mmCplpOelOess.'.. Single ion monitoring was used to determine
QQuuaalliittyy`cMcoeotnnhttarrnooolllssubulnmaumnnaksarryyw:e:re analyzed Sample. MCehthaarncooallblaanndksglwasesrebaenralyfizeedr
pbpelerariinookddsiicccaaollllllyyectttooedeennossnuurrseeitcceoowmmepprlleeettaeelsiiossooallanatatiilooynnzoefodtfahtnhede
sflfoaionmuuennapddrlettr.ooegbbCreeehsbbasllriaaconnonkka..ol faQQnSuudaapnngottiliaantstatstiisooftninbaneoodrffaHfrHadPlPteLLcrCuCrb--vlEaEensSSkMMsfrSScoomddlalaett1caa0teffpdooprrboffnllutuoosorirto1eoc0ch0whe0eemmrpeiicpcaabailsssoioirs abbmnaaaastsleeryiddzxeoodsnnpaittnhkhdeee
lrrieencceooavvreerrreyQyguccarooenmsmtspiipatoarantriiissoooonnfnoss5f.. GpoCi-ntMsStapndeaardkiscubravseesd foronmthe10repcpobvertoyof10k0n0owpnpbspoirkemamatoruixntsspiikne
thesameQsuaamnptliteamtiaotnroixfaGnCd-oMnSthpeealkinseiasrrbeagsreedssoinonthoefrepcooivnetrystoafndkanrodwcnursvpeiske amounts in
the same sample matrix and on the linear regression of 5 point standard curves.
GIInnCss/ttMrruaumsmseennSttpaaellctssppreoecmcieifftiieccrss:s: GHoCw/lMeatstsPaScpkecatrrdoAmeTtDers400
Gas
Chromatograph
and
Mass
Spectrometer
HewlettTCC-eoPomlalupucmemkrannra::dtuAreTDram4p0:0 Ga1J5s&&0ChWWCr0oDDmB2aB-t5-6o06g22rC44a33@p0h0m2ma0ndCMmaisns Spectrometer
Temperature ramp:
Carrier gas:
2H520e55l00CiCuCtmohhoo2ll5dd05Crmai@inn 20C!rain
OSCSpvpaiierkkrneieetrvveoogmllapuusemm:reae:t:ure:
2H5elpiuLm 21580~tLC
DTOTearvasepponlrldotoewwtmipmtteeeem:rmappt.u::re: 3-11308000miCCCn.
TTDTeareaapsppohrffibaagssttthi::mtee:mp: Y2Y150ee0ssmCin.
Trap high temp.:
250C
PPaaggee4doorfs5
22779900..00002233
S3MM_MMNN00O042288333388
} .
[1D3T12'211:: TT-66331166.9;FFCCLLeeveeslsiinnNNaiaivveeRRaaims -FFuurrttheer~lvInevsetsitiggaaitioonnooffRRaaDitCRCoAhoFowTwC5COO1DD4EE%.
Appendix 1
DRAFT 5/14/99
PrLLeiisnnseeuttreeemm:ppeerraattuurree:: 221228554CCpsi Appendix 1
PVarelsvseurtee:mperature: TVraalpvehotelmd:perature:
2521228m55.i4nC.psi
TTDDoerenassspoormrhbboonlffidllto:oowwr:e:d: 5522455r0a,mminL4L./4/mm8iiannmu
ISQSoouonuausrrdccmeetoettnmeepimmteoprperaeertrdauat:rtuuerr:ee;: 522145520005,CC448 ainu
QEEInMuMtaerdvvfooatlelcttmesst::epmepraetruarteu:re: 22215552995088CCVV
Interface temperature: 2500C
HHHePPwLLleCCttss-yysPsttaeecmmk:ard Series 1100 Liquid Chromatograph
Column: Hewlett-Paekard Column:
Series
1100
LKKeieqyyussitdtooCnneehBrBeotematasasitiollgCCra11p88hccoolluummnn,, size
22
XX
110000
mramm,,
5 5
wbtmm
ppaarrttiiccllee
FSFollolowvwenrrtaatteeA:::
23s3.i0oz00oemW~ Mmaimnmonium acetate:
SSoollvveenntt BA:: SoSlovlveenntt GBr:adient:
4M2M0e.0et%thmh(aa0Mnno9olal0m%mBoininu8m.5amcietnauttees
Solvem Gradient: 4HRH0oeo%ltlddutmaaott199900004%%%0%BBB Biffnooirr8n.3351mmmmiiininnnuuuuttteteeesss
Injection volume:
RHHoeoltluddraanttt44o004%%0%BB Bffoorrin|11mmmiinniunutuete.te 10
IRnujencttiiomne:volume: l1O3.p5Lminutes
Run time:
13.5 minutes
EMElileecccrttorrmooasspsprrsaaPyylMMaatafssossrmSSppIeIeccatttrrmooommsepethteeerrric pressure ionization (API) mass spectrometer
MicromC`MaMosaasnssesPs lLvLaoytylfnnotxxarmg22e.:.1I1I ssaootfmftiwovsaaprr-hee6er0icvpressure ionization (API) mass spectrometer
MCMSooooudndreeec::evotletampgeer:ature: eHe-ll6Cee0cc.Vttrroosspprraayy nneeggaattiivvee
Tons: ASnoaulryczeetremprpesesruartue:re: Analyzer pressure:
99690.3.2002Cx,%5112006-s,mm49BB9aarr
ElIEeolcnetsc:rtrooddee::
c6crr3oo0ss,ss-5-ff2llo6ow,w499
CCoonncclluussLiiooonnwss::levelsofPFOS foundin controlanimals may becueto respiration of airbome 'hEaEtnFAdFOlOiSnSEgEL,--oOOawnHHd,l,epvfforersolsosmimbolfttyrrfPaanFonssOmffeeSsrrpfoooofufrnattdd~esistitcn mmccaooatnntetetrarrioimaalillnaaffnrtrioimomomano~oofnnmteeahyaannfbiioemmodaadlul est1oo0tuoracarenne.oostpthhierearrtiddouunrriionnfggaissraabmmoprpnlleee
handling, and possibly from sporadic contamination of the food source.
PPaaggees5 oolfs5 22779900..00002244
33MM_MMNN0000442288333399