Document EdgrMQEdg2eJYpBLadbq9BD04

Wildlife International, Ltd. a. ProjectNumber 4544-109 PFOS: `A SEMI-STATIC LIFE-CYCLE TOXICITY TEST `WITH THE CLADOCERAN (Daphnia magna) ADDENDUM `WILDLIFE INTERN3AMTILOANBARLE,QLUTED.STPNROO,J Uz723 ECT NUMBER: 454A-109 SerieUsS8,5E0On.vPiEPrxToonlSmogeNinuetmaalbleEPrrfo8te5ec0t.iT1oe3ns0tA0gGeunidclyines and `OECD Guideline 211 "Thisstudywas reported using a testsubstance purity of 90.49%. "Thetest substance characterization was revised on 9/7/00 and the purity was determined to be 86.9%. The Sponsor requestedthattheresultsberevisedbasedon themostrecentpurity analyses. Theresults presated wer simply oreted by th aio of he wo puri o a actor of 0.9603 No attempt was made to recalculate analytical standard concentrations or `measured concentrations Som cna a data. As such, minor rors de to rounding may be prescot. Atached she revised summarypage fromthefinal reportandthemostrecent certificateofanalysis. E 5 ug 5 Wildlife International, Ltd. Es Pret Number 4544-109 "REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9% SPONSOR: SPONSOR'S REPRESENTATIVE: LOCATIONOF STUDY, RAW "DATAAND ACOPYOFTHE FINAL REPORT: 3M Corporation Susan A. Beach `Wildlife Easton, MInatreyrlnaatnidon2al1L6t0d1. LLTDO, PLRORNCTTENRUAMTEIRO:NAL TEST SUBSTANCE: STUDY: NovaTEST (CONCENTRATIONS MEANMEASURED TEST `CONCENTRATIONS: "TEST DATES: LvemorTest: ssiai09 'PFOS (Perfluorooctane Sulfonic Acid Potassium Salt) CPlFaOdSo:cerAanSe(mDia-pShtantiiacmLaifgen-aC)ycle Toxicity Test with the NegativeControl, 13,21.1,8 22a,nd5 44. mg5 a.i, /L. Negative Control, 1.4,2.8,5.4, 12 and, 46m2 ga.3 i/L | `BEixEopkxegmipmceaaltraTiloSmmTtieaenrrmatnit-ntoaJnatuil~loynA1u2,gAu01s9g9u29,t 149, 919999 21 Days "TEST ORGANISM: SOURCEOF TEST ORGANISMS: AcEOFTESTORGAISYS! Vor LOEC: ware Neonate Cladocerans (Daphnia magna) `Wildlife International Ltd. cultures -- Easton, Maryland 21601 rr 2 mgaill main Wildlife International, Ltd. 5 CERTIFICATE OF ANALYSIS Briet Number 4544-109 INTERIM CERTIFICATE OF ANALYSIS Revision 1(9/7/00) Centre Analytical Laboratories COA Reference #: 023-0184 3MProduct: PFOS,Lot 217 Referen#ce: SD-018 Purity: 86.9% [i Fo NE ssw] ome en NMR Metals (ICP/MS) 2. Magnesium 33.. SPootdaisusmium? 56. NlMiocnkmele ot] ee IEEE die R|EEE Si E ae aa a HnE S eee e Ps L Bro ea ntna Positive ssw 2 000twie 3+. 614389 wt0iw9t% 567. ._0<<.000.000005l1wwwre/mnitt%% lP a"Tmot% aImlpuri E E ee ] eBaweies) "Tota%l Impuri Errno [ro [TLams| enw `Related Compounds -- Een [Residual Solvents(TGA)| ="Pn iiLm0 7]NoneDetected | Inorg1a.nicChAlnoiroindes(i 52.. FBlruoomriiddee 45.. NNiittrriattee 67._SPuhlofspsh*ate OrganicAcids * (00) 1. TFA 2. PFPA 43. NHFFPBAA `ElementalAnalysis: 231... CHNiyatdrrrboooggneenn 4 Sulfur 5. Fluorine T ; a ma geo B i tn ela a Lim oe | Tee ddr PEOTEMSREER ELE Ee SLan ake cr | 231. `TTThhheeeooorrreeetttiiicccaaalllVVVaaallluuueee===001%%7.8% 4. TheorVealtuei=c5.a95l% S. Theoretical Value =60% 21 <000519swwimm%% 5 0080 whut 54.. <<00.000069wwtt/hwt%% 7s8a 76wtm im% 1 <01wt/wt% 2 <0lwmY% 43 o02l8 owwnmt% 251. 01122447488wwtet/hwhat%% 3 ssw 5. 54.1wtm% conaisa Page 103 . CeIntNreTAEnaRlyItiMcalCLEabRoTraItoFriIesCCAOTAEROefFereAncNeA#:LY02S3I-0S184 Dateof Last Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen 0-10C Re-assessment Date: 08/31/01 F"lPuuorriityde=, 10.0509%%+- N(sMuRm oifmmpeutriatlieism,pu1r.it9i3e%s*,o1r.ga4n5i%c+aLciCd/iMmSpuriimtpiuersi,ti0e.s,388.%4t1P%O+AnAo,rganic 033%) Total impfuromraliltetstsy= 13.09% Purity = 100%- 13.=0869.9%% potassium is expected in this salt form and is therefore not considered an impurity. purity by DSC is generally not applicable to materialsoflow purity. No endotherm was observed for this sample. "iSnuolrfguarniinctahneiosnammepltehoadppceoanrdisttioonbse. coTnhveeratneidontoreSsuOl4t aagnrdeehsenwceelldewtietchttehdeussuilnfgurthe doenttehremirneastulitosn,itnhethSeOe4leimsennottalcoannsaliydseirs,edleanndiimnpgurciotnyf.idence to this interpretation. Based TFA HFBA NFPA PFPA `THreipftlaufolruooarcoebtiuctyarciicdacid Nonofluoropentanoic acid Pentafluoropropanoic acid Theoretical value calculations based on the empirical formula, CaF17S05K" (MW=538) "This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160) COA3-018A Page 2013 INTERIM CERTIFICATE OF ANALYSIS Centre Analytical Laboratories COA Reference #: 023-018A LC/MS Purity Profile: Wmpuiy 7 wewtn es 8.41 Note: The C4 and C6 values were calculated using the C4 and C6 standard calibration curves, respectively. The CS value was calculated using the average response factors from the C4 and C6 standard curves. Likewise, the C7 value was calculated using the average response factors from the C6 and C8 standard curves. Prepared By: David S. Bell Date Scientist, Centre Analytical Laboratories Reviewed By: John Flaherty Date Laboratory Manager, Centre Analytical Laboratories CoAn3-018A Page 3of3 ilCOdTlOiXfICeOLIOnGYt&eArNAnLaITtICiAoL TnEaSTlIN,G SELRtVIdCE.S `September 27, 2000 M3sM. CSourspaonraAt.ioBneach E9n3v5irBounsmhenAtvaelnuLaeboratory 3M Building 2-3E-09 PS.tO..PaBulo,xM3i3n3n3e1sota 55133 Dear Susan: Enclosed please find Addendumsfor Wildlife International, Ltd. Project Numbers 4544-101, 454A-102, 454A-103A, 54A-104, 454A-105, 454A-106, 454A-107, 454A and-4514A0-18 09 Ifyou have any questions, please let me know. Sincerely, DErioes m KSeunriotrRBDiorlootgairst 8598 Commerce Drive Eston, Maryland 161 Tel 410-822-8400- Fx 410.822.0632 Em ecotonwidienternaionlcom Erp Of: Ovroesereg 1A. WEAN Nien Toe Habart Ta HORSE Fox NSRTS Ema dedomtoriot