Document EdZqL2D1KnYaREgb3pO7y7O8L

BACK TO MAIN MICROBICS' MICROTOX TOXICITY TEST TEST SUBSTANCE______________ Identity: Perfluorooctanoic acid, tetrabutylammonium salt; may also be referred to as PFOA tetrabutylammonium salt, tetrabutylammonium perfluorooctanoate, N2803-2, or as a major component of L-13492. (Octanoic acid, pentadecafluoro-, tetrabutylammonium salt, CAS # 95658-53-0) Remarks: The 3M production lot number was 2. The test sample is referred to by the testing laboratory as L-13492. The purity of the sample was not sufficiently characterized, although current information indicates it is a solution of 44.9% tetrabutylammonium perfluorooctanoate, 27.9% water, and 27.2% isopropanol. The following summary applies to the test sample as a mixture of the test substance in an isopropanol/water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD:___________________________________________________ Method: Microbics' Microtox "BASIC" Procedure GLP: No Year Completed: 1995 Species: Photobacterium phosphoreum Analytical monitoring: pH, light output Replicates: 2 Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics Corporation, Carlsbad, CA Test Conditions: Dilution water: Millipore Milli-QTMwater. Stock and test solution preparation: A primary 2000 mg/L stock solution was prepared in Millipore Milli-QTM water. The pH of the stock solution was then adjusted from 4.7 to 6.7 using 0.1 N NaOH and an osmotic adjustment was made using 200 NaCl dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL glass cuvettes. Number of replicates: Two Number of concentrations: Four plus blank control. BACK TO MAIN Element Basis: Percent light loss. RESULTS_________________________ Nominal concentrations: Blank control, 125, 250, 500, 1000 mg/L Element value and 95% confidence interval: 5-minute EC50 = 630 (590 - 665) mg/L 15-minute EC50 = 300 (270 - 330) mg/L 30-minute EC50 = 260 (220 - 300) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical component alone. CONCLUSIONS_____________________________________________ The test sample 30-minute EC50 for Photobacterium phosphoreum was determined to be 260 mg/L with a 95% Confidence Interval of 220-300 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_____________________________________________ Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number N2169, 1995 OTHER___________________________________________ Last changed: 4/01/01