Document EdJz7vjXx2VJDe6QXGZDYJ3K0

BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredtoas PFOS, FC-95, or as a component of FC-3003. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notgiven.The testsample isFC-3003, identifiebdy the laboratorays R1331. Currentinformation indicatesitisa mixtureof 1% PFOS, 15% diethyleneglycolbutylether, 79.39% water,2% Sultonefoamer,1.5% sodium octylsulfate,1% sodium decylsulfate0,.06% sodium laurylsulfatea,nd 0.05% benzotriazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectdegradation of the fluorochemkat component of the testsample. METHOD: Method: OECD Guideline. Type: Aerobic GLP: No Year completed: 1997 Contact time: 28 days lnoculum: Polyseed NX, LotD257, PolybacCorporationB,ethelem,PA RESULTS Chemical Oxygen Demand (COD): BiochemicalOxygen Demand (CBOD): 5-Day: 1O-Day: 20-Day: 28-Day: 377,000 mg/kg 132,000 mg/kg 247,000 mg/kg 296,000 mg/kg 368,000 mg/kg Remarks: Testingwas conducted on the mixtureas described inthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS No conclusionsgiven. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking= 2. Thisstudymeets thecriterifaorquality testing.However, the sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportion in solution. REFERENCES Thistestwas conducted by AScl Corporation,DuluthEnvironmentalTesting DivisionD,uluth,MN atthe requestofthe 3M Company, Lab Request number R1331,1997. OTHER Last changed: 6/26/00 ASel Corporation/ASel-Duluth Environmental TestingDivision Submiftd to 3M Company - 8/97 ASci-DETD Study ID #5010-069 R1331 BOD REPORT FOR 3M COMPANY Submitted to 3M Environmental Technology and Safety Services 935 Bush Avenue Building 2-3E-09 PO Box 33331 St. Paul, MN 55133-3331 Prepared by ASci Corporation/ASci-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, Minnesota 55811 Sponsor: 3M Company Sponsor Study ID# R1331 AUGUST 1997 COD = 377,000 mg 02 / kg chemical. Dates of BOD test:7/18/97- 8/15/97. Water bath temperaturerange duringthetest:19.8 - 20.6*C. Lot number of PolyseedNX: D257. TABLE 1. BOD DilutionInformationforR1331 Sample ID (mg/L) 10 is 25 50 Stock Solution Concentmtion (mg/L) 1000.0 1000.0 1000.0 1000.0 Sample Volume (MI) 3 5.4 7.5 15 ASci Corpomtion/ASel-Duluth EnvironmentalTestingDivision Submittedto 3M Company - 8/97 ASc-l-DETD Study ID #5010-M Sample Concentmtion (mg/L) 10 18 25 50 TABLE 2. BOD Blank CalculationfsorR1331 Sample ID Blank Blank Blank .1 BI..k FIRST REPLICATE Day InitiaDl.O. FinalD.O. D.O. Drop 5 8.90 9.84 0.06 10 9.90 9.45 0.45 20 8.90 28 L 8.90 1 9.30 1 9.43 0. 6WO 0.41 SECOND REPLICATE InitiaDl.O. FinalD.O. D.O. Drop 8.91 9.91 9.91 8.91 8.90 9.42 8.42 8.29 0.01 0.49@-0.49@--0.62-. Sponsor: 3M Company Sponsor Study ]D# R1331 AScl Corporation/ASel-Duluth Environmental TestingDivision Submitted to3M Company -8/97 ASel-DETD Study ID #5010-M TABLE 3. SOD Seed CorrectionFactorCalculationfsor R1331 Seed Cone. ndisoule 10 10 10 10 FIRST REPLICATE Day Initial Final D.O. D.O. D.O. Drop 5 8.85 5.40 3.45 10 9.85 1.89 6.96 20 8.35 ___1.85 0.98 0.72 7.87 8.13 SCP 0.69 1.39 1.57 1.63 SECOND REPLICATE Initial D.O. Final D.O. D.O. Drop 8.90 5.35 3.37 8.90 1 2.40 8.90 9.90 <0.5 0.64 6.50 -- 8.26 SCF 0.67 1.30 1.65 Average SCF 0.69 1.35 1.57 1.64 a SCF = (D.O. drop * ml seed in test) mi seed in seed blank. TABLE 4. BOD Reference8and Tox Blanks CalculationsforR1331 Conc, mg/L Day SCF 0 5 0.68 50 5 0.69 FIRST REPLICATE Initial FirW D.O. D.O. D.O. Drop Coffected Drop BOD mg/Lb 8.94 8.98 4.04 <0.5 4.90 9.49 4.22 7.90 211 > 390 SECOND REPLICATE Initial Final D.O. D.O. D.O. Drop Corrected Drop 8.90 4.40 4.50 3.82 8.95 <0.5 8.45 7.77 BOD mg/L 191 >399 a Reference dilution. 150 mg glucose and 150 mg glutamic acid in I literBOD dilutionwater, tested at 2% b BOD mg/L (correctedD.O. drop) 300 ml BOD volume 6 ml reference. Sponsor: 3M Company Sponsor Study ID# R1331 2- TABLE 5. BOD CalculationfsorR1331 ASci Corporation/ASCI-Duluth Environmental Testing Division Submitted to 3M Company - 8/97 AScl-DETD Study ID #5010-068 Cone. Day mgtl to 5 SCF Initial D.O. Fil 0.68 8.94 FIRST RULICATE Final D.O. D.O. Drop Cormcted Drop 6.32 2.62 1.94 BOD mglkr is 5 8.90 7.00 1.90 1.22 -- 25 5 9.91 3.64 5.27 :;@ 50 5 O.U 8.95 3.20 5.75 4.59 5.07 194,000 101,000 Mean 143,000 SECOND REPLICATE Initial Final D.O. D.O. D.O. Drop Correctod Drop BOD rag/kg 8.90 7.10 1.80 1.12 9.91 9.92 9.9 6.74 6.70 1.65 2.17 2.22 7.30 1.49 1.54 -- 6.62 132,000 Mean 132,000 )Oowl 10 10 1.35 8.94 5.42 3.52 2.17 217,000 8.90 4.96 3.94 is 10 1.35 8.90 3.24 5.66 4.31 239,OOD 8.91 3.27 5.64 25 10 1.35 8.91 1.72 7.19 5.94 234,OW 9.92 1.50 7.42 50 10 1.35 8.95 < 0 .5 8,451 7.10 1 > 142,000 8.95 <0.5 1 8.45 Mma 230,000 2.56 4.29 6.07 7.10 259,000 238,000 243,WD 1 > 142,000 MeaLn247,000 10 20 1.57 1 9.94 4.50 4.44 is 20 1.57 8.90 2.12 6.79 25 20 1.57 9.91 0.72 9.19 50 20 1.57 9.95 <0.5 2.87 5.21 287,000 1 8.90 289,000 8.91 6.62 265,000 8.92 g.95 Mean 280,000 3.75 5.15 2.54 6.37 0.74 8.18 <0.5--f -- 3.58 4.80 6.61 358,000 267,000 264,000 Mcark 296,o0w00l 10 29 1.64 8.94 4.W 4.94 Is 29 1.64 8.90 2.12 6.78 25 23 1.64 8.91 <0.5 -- 50 1 28 1.64 8.95 1 <0.5 1 3.30 5.14 -- 330,000 236,000 -- M"n 308,000 8.90 3.12 5.78 3.91 1.47 7.44 9.92 <0.3 -- 8 1 <0.5 I '95 BOD = correctedrop x 1,000,000ttestconcentration. Omitted data12oints: D.O. drop correctefdorSCF too low (<2.0). Final D.O. <0.5 mg/L. 4.14 5.80 -- 414,000 .322,000 Mem 368,000 Sponsor: 3M Company Sponsor Study ID# R1331 -3-