Document Ed2ajr81Z8936g8rR9mK8y72V
AR 226. 0709
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-85, or as a component of FC-201F-X. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 1. The test sample is FC201F-X (F-11129). Current information indicates it is a mixture 3% PFOS, 36% diethylene glycol butyl ether, 36.6% water, 8.25% acrylic foamer, 7%
sodium octyl sulfate, 6% sulfonated alkyl ether salts, 3% triethanolamine, and
0.15% toluoltriazole.
The following summaryapplies to a mixturewith incompletely
characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample.
METHOD
Method: OECD Guideline 209
GLP: No
Year Completed: 1992 Analytical monitoring: Dissolved oxygen concentrations, temperature.
Statistical methods: Not applicable.
Test organism source: Activated sludge mixed liquor collected from the
Metro Wastewater Treatment Plant, St. Paul, MN
Test condition:
Dilution water: Millipore Mill-QTM water
Synthetic sewage: per OECD method
Reference and test solution preparation: A 500 mg/L stock solution
of the reference substance, 3,5-dichlorophenol, was prepared. The pH
ofthe reference solution was measured to be 7.8. Test substance was
added directly to the test vessels.
Test vessels: Not given
Numberofconcentrations: one plus blank controls, all in duplicate
Temperature: 17.7 - 19.0 C
Total suspended solids and pH on dayoftesting: 3.4 g/L (TSS in
final: 1.36 g/L. Initial pH 7.5 and final pH 7.5.
cEolnesmuemnpttiBoan.sis: Respiration inhibition as determined by oxygen
Remarks: Uptake rates corrected to 20C for calculations.
=
RESULTS
----
Nominal concentrations: Blank controls and 1000 mg/L, all in duplicate Element values: 30-minute EC50 = >1000 mg/L
0004900
3-hour EC50 = >1000 mg/L. Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test
Substance Remarks field. The values reported apply to that mixture and not
the fluorochemical proportion alone. CONCLUSIONS The test substance 3-hour ECs for activated sludge respiration inhibition was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
pATAQUALTY
Reliability: Kiimisch ranking 3. Several pages are missing from this study. `Sample purity was not properly characterized and the study lacks analytical `confirmationofthe amountoffluorochemical proportion in the solution.
REFERENCES
00000000
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number K1126-1, 1992.
OTHER
Last changed: 6/26/00
000401
-
Type Test OECD
ENVIRCNMENTAL LABORATORY ACTIVATED SLUDGE MIXED UQUOR
WR _Kige-l
Date 3/23/42 Analyst _ oN
Page)of 7_
Acrwargn Suwoer Resecamon Tuwamm Tes 209
ACTIVATEDSLUDGEMIXEDLIQUOR
Date Obtained _3/23/42
oate Used 3/23/22
-
Mixed Liquor Suspended Solids (MLSS)_2, 4 gL Adjusted to _-- _ giL
MLSS In final test solution __1-3b _ gn
Initial pH 7-5 (1st test soln. prepared)
Final pH 25(astsoln.prepared)
Comments
TESTSUBSTANCE
Sample Description __EC= 201 -X Initial pH of highest conc. tested (10CC g/L) =
comments _ Boot wey
T-M29 2.0
ler | Adjusted to pH _
'BEFERENCESUBSTANCE
Sample Description
2,5 = Dcnwacknmes
Initial pH of stock solution (50C mg) = _Z-%
Comments
"
.
Adjusted to pH _----
EXPOSURECONDITIONS
Test Duration
D Wes
Test Temperawre Range: _11.019.0 C
-
Comments -
00402
Page 227
ENVIRONMENTAL LABORATORY
ERNIE
OECD ACTIVATED SLUDGE RESPIRATION INHIBITETSTIO#2N08
TEST SOLUTIONS PREPARATION
Tost Substance: ___ C= 90|F =X
Sample Description: _ Don
Anays: KON
Date: Bad)
Lignin
F-W39 Lor
TastConc. mgadded
--mgl__ ` perS00ml
U-Lenra
-
1000-4
doco-(y
S00
S00
mLMQ() water
_oerS00mL 284
mL syn. sewage
feedDerS00mi be
mi microbial
inocgeurSl00ummL 200
2%4
po
CC
2%4
Wo
a00 0
Total Volume
--_-- S00
300
Soo
0c0-A 1.7 \OCQ -& tT
(1) Millipore Mili-QR high purity water
000403
ENEVCIRDOAWCITIEVNATTAELDSLLAUBDORGAETROERSYPRATION NHEITIONTEST209 CALCUATIONOFRESTS
[SE ouute: e212t8152
TestSubstan: EQ-201EX
a
30s Exposure
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