Document ELpVvnK3RzK0RJzeKr7oQaj0

PUBLIC COPY 8EHQ-1208-16436 G DCN:89090000069s Via Federal Express Document Processing Center (Mail Code 7407M) Room 6428 Attention: 8(e) Coordinator Office of Pollution Prevention and Toxics U.S. Environmental Protection Agency 1201 Constitution Ave., NW Washington, DC 20004 89090000069/S Dear 8(e) Coordinator: (lTEHQ-06-16436J&EHQ-06-16478 Perfluorinated Aliphatic Carboxylic Acid, Ammonium Salt This letter is to inform you of the results of a chronic Daphnia magna study with the R&D test substance referenced above. The effect of the test substance (purity 84%) on the survival, growth, and reproduction of Daphnia magna was assessed in a chronic, unaerated 21-day static-renewal test in accordance with the test guidelines (U.S. EPA OPPTS 850.1300; OECD 211). Nominal test substance concentrations selected for the study were 2.5, 5, 10,20, or 40 mg/L. The corresponding mean, measured concentrations o f the test substance were 2.13,4.17, 8.13, 16.2, and 33.0 mg/L. A dilution water control was used in this study. The test substance was not detected in the dilution water control. On day 21 at study termination there was at least 90% mobility in each test concentration group and the control. The EC50 for adult survival was greater than >33.0 mg/L. The Fisher Exact test and the CochranArmitage trend test determined that the NOEC for survival of adult Daphnia magna on day 21 was >33.0 mg/L. The NOEC for the number of live young per surviving female on day 21 was 4.17 mg/L using the Jonckheere-Terpstra test. The NOEC for the number of immobile young on day 21 was 8.13 mg/L while the NOEC for the first day o f reproduction was >33.0 mg/L. The NOECs for the number o f immobile young and first day o f reproduction were determined using the Jonckheere-Terpstra test. A standard one-way ANOVA was done on the data reporting the length o f surviving adult Daphnia magna. The data were found to be normally distributed by the Shapiro-Wilk test. The Jonckheere-Terpstra test was used to determine that the NOEC for the length of surviving Daphnia magna at test end was >33.0 mg/L. A standard one-way ANOVA was done on the data reporting the dry weight o f surviving adult Daphnia magna. The data were found to be non-normally distributed by the Shapiro-Wilk test. The Jonckheere-Terpstra test was used to determine the NOEC for the dry weight of surviving Daphnia magna at test end was >33.0 mg/L. The overall study NOEC (no-observed-effect concentration), LOEC (lowest-observed-effect concentration), and MATC (maximum-acceptable-toxicant concentration) for the test substance, based on mean, measured test concentrations and the total number o f live young produced per surviving female, were 4.17 mg/L, 6.15 mg/L, and 8.13 mg/L for Daphnia magna exposed to the test substance for 21 days. Sincere' \,, Com pany Sanitized 8EHQ-06-16436 8 EHQ - 0 6 I 64 36 3 i& t> 7 7