Document ELkp6K5G5a7rzJ0DovX3yZoR

ARR_2 066 64 FOSA or PFOSA Ongoing - | wl 3M MEDICAL DEPARTMENT, CORPORATE TOXICOLOGY Protocol for Study No. T-7132.2; ST-39 PHARMSTA UDYC OFPO ERFK LUOI ROOCN TANE ESUT LFOI NAMC IDE INRATS Study Objective: `The objectiveofthis study is toassess the potential for oral absorption, urinary and fecal clearance and biological persistenceofPerfluorooctane Sulfonamide (PFOSA) in male Sprague Dawley rats afer a single oral dose. Analysisofthe serum, liver, urine and feces forpotential metabolitesofPFOSA will be performed by LCMS and perhaps other `methods. Previous studiesofthe N-ethyl derivativeof PFOSA have concluded that PFOSA is the ultimate metabolite in ras (1,2); however, the analytical technique used in thesestudieswasunable to detect all potential metabolites, including perfluorooctanesulfonate (PFOS). No pharmacokinetic studies have yet been done on PFOSA to resolve this issue and search for other possible metabolites in the urine and feces. Recently, validated methodshavebeen developed for the quantitation ofPFOSA and its potential metabolite, PFOS, in serum and liver down to the low part per billion level (3). One conversionof goalofthis study PFOSA to PFOS. is to determine the potential for, ifoccurring, This information will help to explain the the extent of `pharmacokinetics ofNEXFOSE and its metabolites and provide data for proper risk characterization. Research Client: 3M Specialty Chemicals Division 3M Center, Building 236 Saint Paul, MN 55144 Sponsor: 3M Specialty Chemicals Division 3M Center, Building 236 Saint Paul, MN 55144 Study Location: 3M Stategic Toxicology Laboraioiy 3M Center, Building 270-35-06 room SB314 Saint Paul, MN 55144 Study Director: AndreMw. Seacat, Ph.D. Toxicology Specialist 3M Medical Dept. / Corporate Toxicology 3M Center, Building 220-2E-02 Saint Paul, MN 55144 Ph. 651-575-3161 FAX: 651-733-1773 Study Toxicologist: Deanna Luebker, MS `Advanced Research Toxicologist 3M Medical Dept. /Corporate Toxicology " 3M Center, Building 220-2E-02 Saint Paul, MN 55144 000503 : T1322; ST39 PFOSAPK Ph 651-737-1374 FAX: 651-733-1773 ProposedStudy Timeline In-Life Start Date: October 4th, 1999 In-Life End Date: November 2nd, 1999 Analytical Completion Date: TBA Final Report Completion Date: TBA Regulatory Compliance: p`Trhoitsosctouldaynwdilcllabsesipfeiredfoarsmaed"CilnasthseB3SMtuSdtyra"taesgeixcpTloaxiinceodloingyTLOaXboSraOtPor0y95u0n,deSrtraadteegfiicned Toxicology Lab GLP Program Procedure. DTaenstHaMkateesr.iaPrlo:duct Responsibility Liaison 3M Chemicals Division. will fumish high-purity PFOSA. Identification: Name: Perfluorooctanesulfonamide LMootleNcuumlbarerF:orLmu-l1a0:00C9F(P,rSepOa;rNedH;by George Moore, 3M SMD Lab, Bldg 236, April 1996) 9Pu9r%ityp:urAena(4l)y. siQsuablyitGatCivMeSanddetqeuarnmtiinteadtitvheatcotmhpeosstiatriionngamlrateesruilatlstwhaast owveerre rdeevreiavleeddftrhoamt tthhee scionmgploestirtiailo'nHw/a's*6F-5N.M8R% cCrFo3ss(-CiFnt2e)gxra-tSio0n2-anNaHly2si(s.Normal mchoanino)m,e1t8h.y7l%bCrFa3n(cChF)2,)x1.-2%CF((CCFF33))2HCCF~F(2C)Fy2-)Sx0-2S-0N2H-2N(HI2nt(eIrsnaolpropyl (brCaFn3c)h,),C-3(.C5F%2)C-xSFO2xx-+N1H-;C(Ft-(BCuFty3l)b-rSa0nc2h-)(N5H)2. (HAPlpLhCa/bMrSancchha)raacntder0i.z2at8i%on oCfitfh1e55P0F:ONHSaA,s$a1m0plpeprmeovfeaCloeFdi9s,S6O0:0NpHp:,m 6o,f6P0F0OpSp,mo1,f10C0uFp1pcmHSofO:NH,, 2c4on,c0e0n0trpaptimoonfsoCf,s4eFv;ecraHlSOot:hNeHr,ami1d,e2s0.0 pBapsmeodfoCnytFh:essHu;mSoOf;NtHh,eiamnpudrlitoiweesr, the purityofthe appendix 1). PFOSA sample wouldbeapproximately 96 % (6 & Stability: StorageDCoocnudmietnitoantsi:on will be kept on file with the Sponsor. Upon receipt, temperature. test material will be stored tightly sealed at room Characteristics: dIenffionrematthieotnesotnmsaytnetrihaelsiwsilmlebtehokdesp,tcoonmpfoilseiwtiitohnotrheotShpeorncshoarr.acteristics that 2 000504 " " T-713P2.R2O;SSAT-P39K. AnimalsS:pecies: Strain: Rat Sprague Dawley Source: Harlan Age at initiationof treatment: `Weight at initiation of treatment: Number and sex: 30 males 6-8 weeks approximately 150-250g "Table 1 - Dose Group [Group [Dose TN ~~ [Futhanasin | and 29 post dose. and 29 post dose IdAeUntAifNicuamtbieorn:: 2ea2r4t6ag with animal number or unique tail mark. Husbandry: Housin`gA:ll atsfromgroup1sand2, which are tobesacrificedonday 29post dose, will be housed individually in metabolism cages for portionsofthe study (see Table 2). When not in metabolism cages, these rats will be `group housed in standard cages. All other rats willbegroup housed in standard cages throughout the study. Diet/Water: Harlan Teklad LM-485 Mouse/Rat Sterilizable Diet, supplied by Harlan `Teklad, Madison, WI, and tap water will be provided to all ratsad libitum throughout the study. Environment: Environmental controls for the animal room will be set to maintain a temperatureof 72 + 3F, humidity of 30-70%, a minimum of 10 exchanges ofroom air per hour and a 12 hour light/dark cycle. Dose and Dosing Procedures: Methodof administration/Dose preparation: A single Smg/kg dose of PFOSA willbeadministered via oral gavage to rats in group 2 on day zeroofthe study. The PFOSA will be prepared from a stock solution of 100mg/ml PFOSA in acetone A final 0.1% (Imo/mi} uniform suspension (or emulsion) ofPFOSA in 2% Tween 80 and 1% acetone will be prepared using a 15 ml dounce tissue grinder. A volume of 5 ml suspension / kg body weight will be administered to each rat. Re`suspensionofsolids will be performed with 5 strokesofthe tissue grinder pestel before each sample is drawn-up in the syringe for dosing. A single 5 PE ~OOS05 Ce T132P2R;OS5A-3P9K ml/kg body weight doseofvehicle, 2% Tween 80 and 1% acetone, will be `administered via oral gavage to rats in group 1 on day zeroofthe study. Observation ofAnimals: Clinical Observations: Each animal will be observed daily (excluding weekends and holidays) for `mortality and morbidity and notablefindingswill be recorded. Additional findings will be recorded as they are observed. `Body Weights: Each animal will beweighed immediately prior to treatment, weekly thereafter and immediately prior to euthanasia. Specimen Collection: Frequency (See table 2): Urine and feces collections will be made on days 1 - 4 post dose Necropsies will be performed on days 1, 4and 29 post dose. Table 2 - Schedule [Sn MaOoc0tns [[aTu[n0ecst1s e>[W[deaOdya2PD[Th[auOaury7s3PD[P[[naOasysPp[[|sOacatya ssen] DOSING | CDlyl1ePtiDon|Caleton| Coleton| CSalcechtioon m 5 oe rn ee im22 lame [Sin[G5 ayi3rp DsycsP> [223E[SE ern Gatien[suiaro] sy 16PD|axy 17PD |ary 18PD | day 197D day207D|day 21PD|day 227D| ans 23% | any24 0 | any 25| ay 267 anDyy229970 Puc Method of Specimen Collection: Urine and feces will be collected from each metabolism cage at the designated times. The initial volume the urine collection apparatus will be ofurine washed will with be recorded, the sides approximately 5-10ml of deionized water and the final volumeofurine wil! be brought to 15 ml with `aandidmiatli.onaAltdetiheondiezseidgnwaatteerd. tiDmaeisl,yafneicmeaslswewiilglhtbewielultbheanriezceodrdbeydfCoOr;eaancdh gross necropsy performed. During necropsy, blood (= 6 ml) will be cwoiltlheocuttedanvtiiacotahgeualabndto.minBallooadorstaamapnldestrwailnlsbfeerareldlotwoebdlotoodclcootllfeocrtiaonpetruibeosd. ` NONS06 tee TNR ST PROSA PK oafce15nt1r0i3f0uagmetinu1t1e0s0 axtgrofoomrtemimnpuetreast.urTehaendsetrhuemclwoitllwbilel be spun down transferred to in rlaebmeolvede a1.n5ymriemmaiicnrionfgugreedtubbleosodancdesc.entEraicfuhgesderaagsaianmpalte2w0il0lx0thgentboe itnrdarnsyfeircer.ed Ltioveasrespwairlal tbee lraebmeolevdedp,olwyepirgohpeydl,enpelamciecdroinfduigveidtuuablelyainndtofrozen labeled stele `maintained on sdarmypilcee. bags and flash frozen in liquid nitrogen then Specimen Handling: S8p0ecC.imeFnosrwmieltlatboelimtepanoalrybsaiesrs,titohlreesyed sipneacfirmeeenzserwisleltbteo pmaaicnkteadiinnd-6r0ytioce-: and shipped to: Kris Hansen, Ph.D. 393M5 EBnuvsihroAnvmeennutea.l Technology and Safety Services PSth.: P6a1u2l,-7M78N-65058113,3F-3A3X3:1612-778-6176. All results wil be provided for inclusion in the final report. a`nTahleynsiusmbaerre,astyfpoellaownsd:dateofcollectionofspecimens tobe generated for `Tab3le- Specimens "Specimen pdoasytl p[odsaty2 pDoasyts p|odsatyd dpeoyst25| Toul dose dose [dose |dose |dose Liver Sigroupiday 30 Urine Slgroupld FSelcgersouplday Data Analysis: tDoaxtiacockoilnleetcitcedpaornampeatreenrts caondmpfoorunstdataisntdiciadlelyntsiifginaibfliecamnettadbioflfietreenscweisllbebtewaeneanlygzreodupfsorusing ANOVA and for Students T-test s 000507 Li TN322:5T39 PFOSA PK Responsibilities: cDoelalnecntainLguienb-kiefer sapnedciAmnednsr,ewpeSrefaocramtinwgilthbeenreecsrpoopnssiiebsleanfdorcdololseicntgintgheanadnismeanlds,ing tissue specimens for analysis. AnKsdirs eHawnsSeena,ca3tMwiEllnvdirraoftnmaeinntaall,rewpilolrbtearnedspeonnssuirbelteheforrepanoarltytrieccaeliveevsalaupaptrioopnroifatthee3M review before afinal report is issued. u00508 6 : AE* A . Signatures: FrosA me T-7132.2; ST-39 --Cndnas MW Andrew M. Seacat Ph.D. "STtouxdiycoDliorgeyctSoprecialist Beacall Mose eGo `DAedavnannaceLdueRbekseera,rcMhSToxicologist Study Toxicologist a "~ J 8! Sponsor Representative: nfazfaa Date soloztrr Date lif? 0050 ' : AE T132P2F:OSSAT-P3K9 References: 1. Grossman MR and Bowen JM. (1990) Tissue analysisoffluorinated sulfonamide pesticide: an evaluationofdistribution, elimination, and potential for bioaccumulation in orally exposed rats. M.S. Thesis, Univ. ofGeorgia, Athens, GA. (also possibly published as: GrossmanMark R. and Bowen JM. (1990) Tissue distribution and eliminationof a fluorinated sulfonamide pesticide in rats Fundam. Appl. Toxicol. but notfound) 2. Grossman MR, Mispagel, ME. and Bowen J.M. (1992)Distribution andtissue in rats during and after prolonged dietary exposure to a highly fluorinated sulfonamide pesticide. J. Agric. FoodChem. 40, 2505 ~ 2509. 3. KJ. Hansen,L.A Clemen,ME. Ellefson, H.O. Johnson (1999). Compound Specific Characterizationof Organic Fluorochemicals in General Population Human Sera Samples. 3M Environmental Lab, St. Paul, MN 55133. 4 Payfer RM. GC/MS analyses of PFOSA (L-10009). SA&C Analytical Request No. 59426. Report 9/24/99. 3M SA&C Lab Building 236-28-11 5. Tom Kestner Chemical CharacterizationofPFOSA, L-10009, by 1H and 19FNMR Spectroscopy Request#s 59426. 3M Specialty Adhesive&s Chemicals Analytical Laborator/y SMMD-236-28-11, September 25, 1999. 6. DeRoosF.L. Characterization of PFOSA Samples, T-7132-1 (L-10009) and TNA-1584. Request # A-151254. Report 10/7/99. Corporate Analytical Technology Center, Building 201-1-29, CATC ~ Chromatography Group. 8 ~ 000510 . Re Appendix I: mTo:awTocoes [RR ---- TPSROST APK cToc ALnadmryeAw SWeeancadliUnSg-CloUrSp-orCaoemlp3oMMIrUUaStSs `Subject: Characterization of PFOSA Sample for Toxicology Lary, p1 rheapvaerecdombpylGe.tMeotohreeH4P1L56C)ItMhSatcihsaprracotpeorsiezdat1ionboeufstehdePfForOtShAe asnaimpmlaef(e-e7di1n3g2-s,tLay-.100|00 found 9,600 ppm of PFOS, 1,100 ppmof G7F1550;NHz, 510 ppm of CaF19307NHy, C6,a6F0105pHp7m5o0fzCNgHF34agnHdSOlzowNeHr3c,on2c4e,n0t0r0aptipomnosfofCsaeFve3r6alHSoOthZeNrHa3m,id1e,s2.00Bpaspemdoofn the umofthe impurities, the purityof the PFOSA sample would be approximately 96 %. idveenttifhicoautigonhoiftheismcaormepaobuonudt wapapsarbeanstedprpersiemncaerlofoCn tghe gobHsSerOvReNdHmoleicnutlhaerswaempilgeh. anTdhe mhoererelpartoibvaebellettohntotrhdisercommtphoeucnhdrosmaatcotugarlalmy.(CRaaFth1e7r5t0ha2nNHC.aFCsonHfSirOm3atNiHonzo.fhthsiesems oidtehntrifpiacarttiioanlwhilycrreqougiernetaedddipoenfalluoarnoaalmysiedse.s m1 aiysoacnteuaeltyobteohifnkthmiosre about whether the hiTyshcecrooPngcFeeOnnaStlreawtdaisaomnqiusdahenotsui,lfdiweobdreeuasscilcnlugqraatues.atannTddhaerduascmiunirgdveae iPpmrFpeuOpriSatrAieedssb.tayhnoadwnaearvldeyrzc,iunrigvnecPlaFudOsinSgsshuteamtpnhdaitarnrtashdlgeosy Sihntatadrnodthdaurcdessasfmooemreemaaacshosorfstptoehceirhsaeelqarmuieadsenpsoi.nssieAefIadScat,otrha as5 c1dailhdceuPlFbaOetSsetdA.wpuerWtcyhanaisdso(uiSmsiensscsestwuhmeapdiailooonnfotwtihaleve N{ihmiposusrtiaimhoepsfleteahrreweasasnmaaelnnyaaslbeyiszeefdtooubnHydPRvLicoCklatPnaedyoiraerrseeudsmeiitnvegocltGetCdib/eMyiSmtphauenridatnibaeylsy,CsiAs9.T.CWhNui-smlieentgnhooylulraFbGOuCSaEym,serNtu-he,. ethyl FOSE, etc. al concentrations greater han 50 pom. Ph1 aeFlOsPoSFcAOarsSraAimedprleoesuptwoaanssseefm0oui.nq8duPa10FntObaeStA1v1es8ta%as.nsdeaTsrhdsemcesunervteao.nfatlUhyessienpsgurwhkieysroeftectcahhenriePqduFeoO,uSttAhibenypduucproltimycpoaaftret,ihnwogih cvrahririoacmtaieotnogwinrjoaepcuhtiiocbnems aeovfteehraaogceh1d0.sqoluuIatnginoeinny.esroraet,lhaehlyoiwsvehevoyeurpl,udrbeiemarnetoleatrtiihavileglhdyluyaecacocucruharelasteeaxs0truaensamleeyltayiclaalrge istinaosctuntmueafrlatchatilogr,vhaenrritathtiiasoncn awtsoheuelp>du1r0bt0iy,as0o0tf0h,heeotuhPraitFlyOmuShsiAgth.tbhaet wapiesplpioeadrs.esiuiIseisntghanoa5tte8hxesptpeacnutderadrodft!htthIenssamwpelree rue, he purty would be determined to be > 100%. hcAaenldcCrauellcawutleSadetatechsaattthtahhleshceroneacveitnetwrreaedtcietoihnveioncfgonP3cFemrOgiSr/awtdiilaonnysotowfoatudhvleedrinsgemlepysutafrpfepec.thh0te.2w0setrumdegy.dPetFAeOtrSm9i.tn6oet0ad0lapn1odm, 5 000511 Lm : TN32P2F:O5STA3P9K Appendix 1 Continued: 2f8ivderay(bsaasesdsonupmreaiv0in.ou2gs5fkegedriantg.stIfuwdieefsu)rtahnedrlaivsesriumseaptphraotx3i0ma%teolfy h1a2tigsrdaempso,sithteendithnethe. 0prleidviecrtweedigcohntc=ent5rautgijognrainm,thoerl5ipveprmf,rowmhtihceh reinsisdeulalfPshFoOuSldwnooutlidbmpeo2s0e0anuygtPoxFiOcoSloxgi0c.a3l/ 12 mcoentsaebqouleinscmeso.f PAFnOySPAFtOo SPFmOeSa.sured significovaenrttlhaytcould be attributed fo Hmeetabbeolliievceasttthaactk,thheow1e-vHerantdhe2s-eHapmeirfdleusorsohaomuiddebse aarnealiyntteirceasltidnigstaisnctthferyommatyhbe esubject to `Pceornfcleunotrriantaitoendasnpdectiheesreofnordeowwonultdhebleinaec,caenpdtawboluelidf nnootoatmheorunsutbsttoitautfeorceasneebaeblperetpoaxriecd. Andrew is sil thinking about whether the (CgF 17802)2NH will have a negative impact on the study. Pplroevaisdeegaidvdeitmieonaalcianlfloartma6t-io0n5.5 1 you have any questions conceming our unalyses or if can CFrceAdTCL. DeRoos 10 anns12. LaboratoryReport Analytical Report of Data for PFOSA Pharmacokinetic Study in Rats (Sera and Liver) Laboratory Report No. FACT-TOX-145 (W2814) Requester Project No. T-7132.2 Testing Laboratory 3M Environmental Technology & Safety Services Fluorine3MAnaElnyvtiircoalnmCehnetmailsLtarbyoTreataomry(FACT) 935 B2u3s6h-A0v9enue, St Paul, MN 55106 Laboratory Contact Kris Hansen, Ph.D, Bldg 23-09 P.O Box 3331 St Paul, MN 55133-3331 Phone' (651)778-6018 FAX: (651) 778-6176 S-------------------------------------------------------- Requester Andrew Seacat and Deanna Luebker 3M Company. Toxicology Services 2202602 St Paul, MN Tors 000512 1 Introduction a tohnheilpsgsatevuadgyrewawlistduheSsmoiggnredtcoopfaroPemlRiicOdmiSena(AaPrsinuFlsOypSeaeAsn,ssdeGesisdiFhiyef2asd%SosOoTrNnpWHtE,iyoECnNA]aSn0d;m3ec1oi5nb0t6or:lo3li2sa.nm8i)bmyaTlteshwteeaSrpergnaigvuieewnDe2mar%vedaeToyWslEeatdEsobNfy da8an0ydia2tn9ahplnoyosztePodFqsOeaS.nAtS.tearSiaueablsneydtsfoovfeprtchroefldllooerscoeodcgfarrnooeumptsauhnldfsotnahaectecio(inPrdFoaOlrSg,iraColuFp,wwSeerOre,seaCnctAriSf0i#tche2ed7oE9nn5vc.i3yr9o-13n,,daFyFO4,SLaAan,bdand perluoroactancse (POAA, GFsCOF, CAS #2991517) POPOuSSp.,abnuPdtFOePvSReAnOlSeAevwrerlfieondmehtaeenlciitmveaedlrsisnosfachtrehiefvdiocressd 2o9fadaailyldsposoowsde:darnoeismhaiwlgeshr.eesPtdFinOetShmleeigvrelosuwpsecraoecnthiaiiignhdeoisgtnhindatyhveed1;asoyonfday {n29e, lthimiatvoefrq agePFOSa Alvthlewavesr raspopfrodxoismei adtaeliy2l.5s9 th day 1 levels.POAAwasotdtetahevde APcRosnOitSnatiAhnlePeFveOeSsrAdeexscharocevsaes,etsfheoremixdtaoyfcd1e1to0elcdotaicyotne4.dPo4OddaAayAysv2o;asostnddedotaseyctve29d,iinsdteheefdsreohrmeofHofiugnrhoaeusmttaPofRcOovSellaleovniealdsl.onScedairydumm|ck PaRnOdSdaAyd4o.sSincg rsoeutgons.cisof hedos aerasuggest hat smallamountofPOAAwaspreseninthe soPlRuOtSiAoBn,us(dC1o0 ydoSsODt:heNHan,imaaslsd.etNeoctevdeisnttihgeatiivoenrsooff PdFoOsSdAaBiilnst.h sThriwsenryetcownadsuaeldsopresetinh "caTolhnsecoeisntordalteinonnuiisnntdth1hei0TddooWfssEeeiEsthNoee8to0dsns.aonlTuithmiaocnowwnacaessndatelrtayetrzimoenidoneftdoPtcoOobnSfeiwr1ma3s30ch-e+tt/h-e4o0re0tigtcoailbldeoa.spepPrlFoeOxveiSlmasanTtdehlPey P1OF8.O5ASwAeA.re amnaagtl/eyrmsiLia;slaPunsOdeAdsAh0lodeuodesbesehaceroanpsnpiedioevraeidnwaa0isbeedreoaqtfues2s.et5eepdgibinymgL3.MqPuTRoaxOyiSocnaollnyo.dgPyAaOmnAodrAwedsatthcoaorwnoedurguehcccohllaberycatFecdetedrorDmirazoasotsifnipgorhleineo 10renstiost. fithoesund. Resultsofthismorecomprehensivecharacenzaionars avaiablefomth. PGArloolcobedoduLuagrbheosrriwagetoorrreoyufPsroqalcultiaocwye,sUc.oNenetairctoqlhumeiersatisteuirodenoossfaetnmdiase3arMaaElnnwvoairsrnohnoemtaennnaetlcayeltsiLscaaablrosyrasctnoodlralyrecdStseiwdnesdrcaecrogdridOvipenenroicanepgnpulfiiccaatbeloef dacneafhleynassiresocdfoipsrerobmdiayutcNzetrMaisRadal.wbTaihsechodaarrtsaacfpirtrie.zeeTndhibidsyhdNraMetRsinatnhodesiLguChaMetafoanltihyesdabauartvoynaab0aslndagarodvisesrGwmeeer.net agencyin 2 Sample Receipt iE fOirvnoezre,1n0aon4d9n9ds,rey1,0c4sea9am9np,lewne1dr0et1hs1et1orF9eA9dDCaeTta-gn2rn0oauCpL.untkhee 3fMrEomnv3iMouTmoexincaolloLgaybSoerravtiocreysshmippledswaeeirneec,efivceed, 3 Holding Times i hE "Thereisno holdingtimecriteriaassociated ith hesesamples forL/ESMSMESalysis. 2086 000514 Methods - AnalayndtPriepacraatorly || Le AnaSElTeySrt-ui8mc-a4f.lom1,eAEtxnhtoaycdtsiioUnsionfgPHoPtaLsCsiEulmPecoepraxy/uMiasSpresroorocerOatyiheraFirnochsemiscallCofmpnnndssfriom LEiTEvSTeS--r6f6:o50r1A,n,EaAnxlayrlsayicssiiUsosonifonPfgoHtaaPsssLisuimCPm-PeeErolsreauocMcearoansoerSnspfeoopcrrraoosomareocOtrytyhtueesFnaFxcoachrsermbliceaflnsioCnoSmerprouaumEndtsxreocm EUTUsSsi-inn8gg:HH7PP0L,LACCEsHleiecsrcooosfspPproartyayMsasaissuSsmPSpepeseurccoormnoeoercytaneuforatc orOtherForeeheical anLiverExtras SaTAmBpyAle)epirenpaeapxrtairtacictooenndtTrforPlolmLesdCeaEnmvSpilrNoeSnmmYaeSntt:i.iTwhniehgciiaotntipoonaciciirnreacgeaingotsenlncicvrealbuytyaragmsmonoinuimcDodrroegeepnesiiclst Ontcyeettheea)n,odniTd,BaAdpriecionfsotrimnegdd,nhe aenaloy isorrMaSnaelsn.o3 onpotarg oven (mydr IHnPcoHLlPCuLEmCnS,BMaaSsnMealdSioqnuohtoefaeinxtcosf nheacnalyntedfoprtshedstihrroeugnhaphaeereiphhe aolsedeharvoeattoroptiicd ansmalilultyopneaxPtsFrOaecSmeafyoalmteesae8eini0tmhinimsiafcnoasrl.yaRsecitsehvnteairormnaaiinccehaseobdeuotwtteheonfHuPsmLeaC.nsFyodtrc PemRO0SwasiliiunmoS1n9annp i h oFdroepltelncoitwicionncogHfmPoLoCmslpaairnpcailiouondonsi,nsgEugfSueoMnrnSroclMhydepmorpioecsvrliasdt.eesdEeaceraopsitparnlady,amacincduostenemameapnseefrhorsaasMrqleyozillngpea ivcrie dusaolnees,of `abdnasdceaapntracyoliloins,iocnhscareceinissaiclofetshecannadey,oisnselTcea.mThsiuotinuise boho paremwsifhonib,ghaehneehrgyd {iFhsoersmeesxgeacmompnldeta,rfyooraPsFtgOhmSen(osCniss,duSe0ct5hec)atea8dnaa0lanydsmicsa,n(cbonoemu4e9espdios1n0sindlgiefe0dSreOsnt,ee FchOaSr1a90criaoermeisti(heepCrimc5aor0ym)opn.uETahhhiostton mSighethaceitohonesaanpmeercdthaairaoancpreny4s99am pryfon,butdiffrent chemical compostionsand 4 Analysis 3 | LE 41 Calibration Qaaunaonnrtcaoursrvosesnpbfreaecikgetediantgg asyhegorsnouipioafnssadhmepnlTlescl.Qaeuregarpesoisnioen oafeanncyohsnarvamteedgeoaf1s)d onf0Stwro xeipcotosdof ihnAsemieimxepdencettae,dldveanxla.cetCeadlmbraatboinccahlebcmkisoanndcahredcskaaencdoamrplasnr.lifyssravmeernyt5-10psounisstwo mionin 30% of sore 000515 4.2 AmBdloiaennkcmsai,cwoeinsmkse,wsliongkvarrt uorogeemraar,m se excd i ihSexnchbocshhofaspe. E isSy, ksweecompl e ---- 43 Surrogates Topnoeinosnrvsua ctsoneytpiantgo1s5pn00rsotr lscaweoasdt1lm5 sceumao:miIensts isirlaoh sSSurpa og,ae pd Gonoeigs 4.4 Matrix Spikes PJ maRaOsSl"pWAisthPhoOonAmeceAdvpa6isoo,PRoovOeSrrsrotvoyerFOsSv.iesArrLwlFiEOnSb5Aem0omtptsialilasrnwg-- viofsF.p1o-- 3re0.s% BOpoateootan erG FORA fom srad er bnve si FORA wl O`ocnorneFsmiFdeOarSesdAscswraeksen5iv0nsg%qurPaleRipOtrySeovnlaoysr. c1a1c5h4abarFgOeRsAnlaty 131%h.e sevetion ty.Sperove 4.5 LaLbaobroerrastCoraynCooSnatmrpolelsSvamptlesc.ompen of isc, 4.6 sSapemorprml.ae inRteshleabctreeadtCoedowrmimeternhirtsyssty,wnilloyrdpaoret eildpponrwcis.e regetfrom he DcogmeeevieThroinspovsnavdalsiyocndsoofdohredTieWeEcaEnNiai8lonosfdFdOlaStliaoonntwPsrOofAsydosfaoinmteamnbonsoneofcPFsOaGAnLdr 5 lieSummary nin EE phre iicnfeassdoi.g he srrlyssis alinArcher A. Reusof he vera se PdR0rSOseoSaarprtndepaPnFolOdSydAyrw3e2nr9ersd0etpwtecrhateiddiglynetshteeelivrveelrd.soeFgtfOraaoilNlndecodastekoodaenoikmHanalasns.PhtF1OhvSvolleenveoohlfesP2we5Or.eSe0oh.ifvgSahFersOtSiAntFlheoPdkayOn4e. PcoRdnsOtiaaSinnyePe4.RvvOSeScrAxedsrocoissnre,sdsuhemeoscimifdcoeefysddecsoceloycl.4e1Pl04OdAygA2wg:tostodoheyettte3el v.iencsodorhmeoioosfgsFhoOltAfAFOwolSvcrslsst.oSoeanet PROS donoto. ostheals Nomest bonsofFFOSAB nes weecomTH -- vars 000516 tThhheeedTsooWsleEutsEiooNlnut8ui0soensd.oTtluhdtieoocsnownachseedntaeetsiteaonnmiemofadlPswObaSeswa1naa3sl3eyzs0et-dim4ao0tcesdotgonlbfe.iapPdpReroOieSmvaaetnesdTlyPhOe18AP.AF5wuOegSriAnecaLoslnoPcOeeAamiileovneilinsn acr soppnrtosobceioifasmc2dr.se5yetngeieAnngdrLaPleOyoSdnanydPOAAdatawerecollie rom singleanysaodshouldbe Jl bh ERi ER ap app ofNtohceieduatmas.Tahnecelsociwsitngduraingqutahleitpyreosbjeencttivedsywehraetfwoolullodwheadvdeuraidnvgetreseslyyat.fctdthe qualityorintegrity + LiVnsewaeriitgyht~iTnhgecomlaion oeficien (2)ofthesandardcurvewasqual 0orgresierthan098 sing + PLRiOmiSAoifndseeterctiison24d7enfign/emdaLs;4PFthOeSLAOiGn)~vFeFOsS2.i1sr5i488ng/mL;PROSinliveris 153ng 5; + PLvRiamOlieSt)sA-oifnPlqFiuOvaSeniriitnsasi1oe2na4i0de/9f.8i7n6endag /tmhLel;oPwReOstSeixnalcitveerdisst3a0nd7anrdgs/egc,rPeFOS0Awiitnhsinr30is%o49f3thenogre/tmiLca;l Duplicaton requencyaccperecpisiaonb<l30e% + sSppiikkeeinrlovqeuren(csysSacoepacbloe.4ne)overies ~ 70% to 130% PFOSrecoverywas66 %for asinglematrix + Useofconfirmatorymethods noconfirmatorymethodswee sed + Dspeemctornalshtuaiornoaspceciificoeiftyrs~isznpgelaecpitrfoiidyuocwainsodnemonstratedbychromstographiceetion timeandmass aaGbniaevlreytnteterhceecphoavireareym,estdeirtsalarsedqaabmotviea,iavned1a0s1s+ur3in0g%spTikheevreacloivdeirtiyeosffUorim asasouuimtpatbiloenniacsantoitobneoefnevnedgoegebnoys 7 Attachments CHEE ++ + AAttaachcmhemnetnABt: TTaabblleeooffrreessulstooffsievearaannaallyyssiiss AtachmenCt:Sample receipt AAtacthmeanDEt::EcSxtracchtionrmrneedsuealnseaolfyntdniocsaelvdtieartiafnicatgion sors 000517 EE B atll 12 e it HB `Kris Hansen,Ph.D,, Principal AnalyticalImvestigator 12/06, 2a Date . 000518 LaboratoryReport Analytical Report of Data for PFOSA Pharmacokinetic Study in Rats (Sera and Liver) Laboratory Report No. FACT-TOX-145 (W2814) Requester Project No. T-7132.2 AtachmenAt `Tableof Results - Sera Analysis 000519 CE TT TT ET EEL EE E EE E a(ees Fle EEL Te[el [TEE] FE LiE e EL EL] Clas EL Te felTL[EEL] le LE[El [e EE Ta] FI aLEE E EE R a] em a 8 nn LaboratoryReport Analytical Report of Data for PFOSA Pharmacokinetic Study in Rats (Sera and Liver) Laboratory Report No. FACT-TOX-145 (W2814) Requester Project No. T-7132.2 e--r-------------------------------- tachment B `TableofResults - Liver Analysis eee 000521 Ee g EE m Tena Epon B ERE Enese i= =Eeree IEE TEE = ElTelet T 1 EE Te[E 2 (IEE Ee Tu E[51 I. EEE Tr PR -- ie CE || ST 000522 LaboratoryReport Analytical Report of Data for PFOSA Pharmacokinetic Study in Rats (Sera and Liver) Laboratory Report No. FACT-TOX-145 (W2814) Requester Project No. T-71322 Attachment C Sample Receipt 000523 rosaries 1 REQSUHESTENVFIORRONAMNEANLTYATLICALLA/BFOTREALTDOROYRK ' LRDEEBPQAURERTSEHTQEOUNRETS:TNAN0Mo2E.272DE8A1N4NA LUESKER PDARTOEJECRTECENIO:VED0:0101500/50080/01998 DSc: ROSA PK STUDY IN RATS TOX-145 CEXOFNTCROAMCPT DLAATBES:):12/30/1990 DPARTOEJECCTOMPLLEEATDE:D:KJ HANSEN aPhHONFEAXNON:o: 63615-17-7787-66-0811876 220-26-02,7-1374 swele owe cook DESCRIPTION| RESULT vol or (95% 6.1.) KH-SPEC SPECIAL STUDY - KUM ` = UPDATED DATA 3n o kovnv Ae 000524 . Tox 148 SaupLes eceneo 2. sSuoass a Gramens cprhass Jp To KC5aosria.A.nA.RCaaBmrabagonG /'UUSSCoCaro rppoorraatteee sM//USUSGSr @A@MMMCCoCo) oprrppaoorraattee -Sbjct: TOK145 SAMPLES RECEIVED Kris J. Hansen/US-Corporate/3M/US@3M.Corporate 10/6/59 we received 10 Liver, 10 Sra, 20 Faces and 20 Urine Samplfeos days 1 42 from T3GM7o1nTOi8Xo5ICd)OLOSGaYm.pleFsACrTe-caTmOeXd-a14s5i. nLoAByREoQnUsEnSd#TnaW2v8B14o.e PsoFrOeSdAiPnKrSotmudsy F16in Rats. oars Joni rod sample weceipt= refolor ~Rars) [-- aw rst Fage 0n0e52S TPoFXO1S4A5PK STUDLYRIENWR2SAITAS DAY1DATE 10/5/99 Gen.MALEPOSTDOSE URINEID OR03240 SORROON!2 ORO263 R04 OORROOII22SS55 ROIZS7 ROSS SROIS9 FECESID OROJ240 OORROONN421 ORO ORO2e4 OORROO2SS65 OROST ROSS OROS0 LIVERID# SROS20 SSRROU322332 SRO ORO OORR0O334456 ORO347 OR0348 ORO33 SERAID 9R03230 OORROO33223M2 R032 R032 OORRO0S32244SE ORO324T OR03248 9RO3240 DAY2DATE 10/6/99 GenMALE POSTDOSE URINEID FECES ID 9oRRO033224410 ORROO33224410 R042 R022 SRO263 SR03243 SRO24 SROG244 : RROOSSSS OSRROO33225%56 SROI2S7 SRO3ZST ROSS ORO32S8 ROS) ORO32S0 000526 | BEST COPY AVAILABLE ShinarCusiley Raouns 1 rom 09303 [Deanna ta An aad pe Hage la ad fo BE ey (F (leD ponapp duoiT m dt)t | |uoomE o tfgdTryg||Enn"mm[AlemaImd [eeiicen [[CellaoGfsmAsttnaosrSearaliit0ssTBoonlloiSmoleorns|||[ | 1 I |T Dat Sy awos wpmraosat) iwioenps)t CC E N -- -- ---- -- p = = O eee fffeell= l TTrT ger e =o [tom [romssigs| me | one]sheave|main| me | ope | S 2 r {pul|r l(or iias | fBilsr tanens C ti IrrrS] EC TOK 145 SAMPLES RECENED - Barbara A. Gramenz [ fcryas-- s 7 10/11/99 11:54 AM . To: LGKiarcraianAJ.t&,RCalPamamubelon///UUUSSCSo.CrCoparorprpoaatrreaa)tteeM///S3MUM//SUUGSSI@@3M3MM.CCCooorrrpppooorrraatatetee Sibject: Kr J. Hansen/US.Corporate)3M/US@3M TOX145 cays 384 SAMPLES RECENED Corporate SdOtnaudy&1y0/i1n80/R/9a89t/sw9e5(1rr7eo1c3em2i.v32e)Md.T10OSXLaIimCvpeOrlL,eOs1G0Yr.eSceeaiF,vAeCd2T.0frTFoeOzceXens1o4an5nd.dry2L0iAcUeBraiRnndeEhSaaQvmepUlb#eesEeWnfooSsrtLdoT.raed3yPinF1FO0rS/e7Aes/Pe9rR9& FEA 20m 100. aa Samp VeOiRE tomar, _ printed 10/11/99 : (om Restricted) "page 0005286 fPRuOSiAPK STUDY INRATS DAY3 DATE 107/35 Gen. MALE POST DOSE RUR0N2EI0D osRora2ot RoRiu2ze Rsorsss SsRRosSzsTe Rms FsReOc%e2s4DD8 ooRro%2oe1 ooRr0aoes oaRoosse ssRmooaaazssTs smose DAY4DATE 10699 GenMALEPOSTDOSE SURRNOEI}O sSRoUSez1 SSRRMO RSRoussss sRouusss R025 SFRECOES.IOF RsRojO2et SSRROOs) RSoRwOsSeS cRroowsss 9R0325 LSVERRIODH oSERR0ASI2DK soRRoOGRZyI ooRROO33aZdS7 OoRROo OoRRO03sZE0 ooRoOws)t SsRROo3sZeSstO somooawnsss smo SRO (DIR0ILS THERE ARE TWO QRO3SY SERA SAMPLES ORE SHewd BE ARO3253 BUT DONT Kio) LOHICHCRE Be tofu. Ky Lae 000522 TPROOxS1AsPK |STUDLYREIWNaRsAITaS DAY3 DATE 10759 Gen. MALEPOST0OSE SURRNEOIDK SFReoc3ezsd0OF RRoisezr ssmmooszteer RsRoosmesa ooRmoossa2seea saRuosses oommoosszzsses i ssRRoonsseT somnooazzsse? sRomss omoszss DAY4 DATE 10899 GenMALEPOSTDOSE RURONEDIDW SFOECMESOID# RLVOERSD# OSREOR3A2ISD6H SSRRS SRO: osRRoOweezt RomOokTss sshRootszsi?e oRONZ4d GRO GR0S2%8 RcoOmSeSs SROSS SomRnOess RoOmoSws)s Romoossse omoazss oRoist onoszst - RSRROss? smmooaazsrh ooRRoo3wzssz3 oRoss2 ROS) SRG 9ROIZS (IRISH THERE ARE TWO QRO3ISY SERA SAMPLES 4TO2NhEEavieSbuaun3a6deidns@BSE4e,ah2RaosSAauo5Ya3mnBgseU.rT sAeDmOoNloaTlsinK@ aoq0325L4OHiInCoHOrRdE. BAG, ofufen 000530 BEST COPY AVAILABLE Sh Susyfoeore 09600 [Bt rgicle| Cor m tut T [e gm | 110SmTeaep | [pmeesdE)Gomes| [nzAsncn lidsDali] | 11 [| Tio [iol Bee Ere Coy alte aleg||rTTeT] C[ [elof leye5 scteeses r ToeilllaaamlmL[ashcoeems[T{TITTTTTTTTTTT[TJloo]c|(PRR --a--ss--y----] Ha E P EY -- rr = r I---- he I Lol ---- FL LL IIT [ Q[emt neknosnedyntasion[time | One |shispeais| coedsyAtiston | Tie | oui pe eh en ein R rr r r C -- -- D&D LC oL loglay T"1 SowRT everseSide ForInstructio|ns ox 105a 29 sarrece = . BR arcaran S Gramens osuossen To. Kar Rambo/usCaparaeSW/US@3MCorpor S c: GrantA. Lisa A. C T0x145 Paul/US-Corporate/3M/US@3MCorporate doy 29 SAS MPLES Reaca ENEoDe o cos lemen/US-Corporate/3M/US@3M.-Corporate aE nds SO er om AeBre ES eee an On 11/2/99 we received 10 Liver, 10 Sera Samples for day 29 from 3M TOXICOLOGY. FACT. TOX so Simples vers hid= 1/atos pon) 000522 PFOSA PK STUDY IN RATS Tox LREwW2s1a DAY 29 DATE 11/2/09 GenMALE LVERID# SRO240 9RO3241 R042 SORROO224643 SRROOII2SSS 903257 9R03268 ROS SERAIDH OROI240 ORO3Z261 R022 SSRROO322e443 OORROOIIZZSSES GRO3ZET R028 ORO3250 POSTDOSE 000533 FnTmeg | htm 00305 [Pompe Luci ] es ee cs Ts URES Mame| y omen (fr oti wn [aaa][ | Rosemanof foes HP HE-------- A| i 1 a 1 En-- H i E-- E-- ] bb e|r I r e 1] HH foe e bl TT-- Ht -- T -- r1 ] --f----+--+--+--+----+--1| : -- -------- ---- LC e 1 TT 11 1 | Laboratory Report Analytical Report of Data for PFOSA Pharmacokinetic Study in Rats (Sera and Liver) Laboratory Report No. FACT-TOX-145 (W2814) Requester Project No. T-7132.2 Attachment D Screening ResultsofDose Verification 000535 & Terme Kris J. Hansen && TTaeomons p ------ To: Andrew Seacat/US-Corporate/3M/US@3M-Corporate Deanna J. Luebker/US-Corporate/3M/US@3M-Corporate cSei:biocs TOX145: PFOSA PK. Goss Vecaton Lisa A. Clemen/US-Corporate/3M/US@3M-Corporate Hi Andrew and Doan: WPdFaeOthSaaAsvewscacrsoomadpilenetgtaeiQdnuoeoudlryisaanpadlnfaohtfissthadeontPdaFPOpRoSOsASesdaoansndeyPilnOoTAWArEfEQNure.staiEorirnnosrcablbaoaurtnaitehla.hbtoeurtpr+la/ot-as3ti0o%yn.otfhihs e ronacsdaane owfsthmsayPOnAoAdomnotrswdaGtaapnottsmfoomheaP.ROS and POA. However, | check for th APVReiOtS.i.e"dinAyaleeyo,fobfthuyeoiduaratrvaotiicshetnomvlssswi,4d0aAannmcdsrneocfwa,PtEoeOusSAtmhapahttoihtoreonasco16ncbPseRnOpSha.bootSuoitnecpsheoottfholFypsFihOsoStoAofltyPoaFlPOOSAAAto[not SCxopoetrmwainhtsYowuarsCaolnceuc(tatd0u0n5derPtwiTyoraarfaoowudsoeonndyieioanrs:: | an hk vo pave ue 0 Woy In ween so: PreRcOoSveAry 130% 120.26o%f expected dose of 1000 sgn. > 1330 sgimt ae TPRRooOnSrny1 111888%% of expectad dosn of 104ml. > 18.8 ugiml -> 28mm she sive covery 121% Lot me know you have questions. in d00526 mT C2 a Torressovnasea pu S Ca0 MrovdoSe Deo.yToss Sons STToanGehbvearen To: Kris J. Hansen/US-Corporate/3M/US@3M-Corporate cc: Fred L. DeRoos/US-Corporate/3M/US@3M-Corporate Deanna J. Luebker/US-Corporate/3M/US@3M-Corporate Subject: PFOSA dosing solion Sue Chang/US-Corporate/3M/US@3M-Corporate sskoilsuw,tionwecrheecdkiesdcufsosrinPgFOinSoAurcoansctenFtraitdaiyo'ns,maenedtifnogrwFeOSwoaundd iPkFeOAt,o hwahviechthmeaPyFuOnSdAerdocsairntga pcSoronomdbeiltaiPmoRnwOsSiahicostnechefnrTtoremaetpihnoont8so.l0yGsciosnn.caWnetrhaaettfatnhtebhsetidanorgsii1nng0gmsioogrtehrtofolonahwlsiohwapyspourufo.iwcm|aauntntedledyrist1ti3a%onndPFftOrhSesennsoiy5ecsouodf ptohsesidbolsei,ngthseonlupteironhaspasmpwleeCboeuladnaalsykseFdretdo.DesersooIsf h1erasna1iySeisghneicdaenstiyngmosroeoBnF?S in there? If not TShtoecdkoPsiFnOgSsAouitsocnetwoanss pwraespamreaddasupfoallo5ws3:mg PFOSAD.52 ml. acetone = 100 maim TFhiniasl wcoanscednitlurtaetdionntoof5P1F.O4S8A.mi=of112%mmTween 80 t acheive the dosing solution FFiinnaall ccoonnes oTfweaceento8n0e == "12%% TAhvainrkosn, 55527 EE-- Ere r -- e Ee SE pei fom |athe" pazob Pork WTWEEN 309 po PFOS in TWEEN 20m = ep pn SAT A gZ3Q 1 PavEerRs LFfuO =0 = _ "25w0e0"ry 5 2FpH oe Study Date Analyzed InFislter#ument PFOSA in 2% Tween 80, Sample TN-A-3046 [F10l2e5r9013 10200014 10209017 [MSteaanndard Dev. %RSD TOX145 10/29/99 Soup100220919999a T% Recovery To3f 2D6o4 se|Roef Dcosoeavmte(2p6rp5b.2)y7|] 134.31 268.62) 13283 265.65) 13039.2167] 0.688 2661..5813]] 0.688 expected 290 re Ve covereh 2S ere PPosh = 3 anf Comedic spihe 00539 #FOSA dosing sation TP"+ Aondrreewsso0n3c:a5t3 pM 2SeMocCtMeodnbithcaS2oo2i0p2.6.PT0oo3sicogy Sories TFSoeos asl,oarn i7e013.81e7748 To: ce: Subject: Kris J. Hansen/US-Corporate/3M/US@3M-Corporate Fred L. DeRoos/US-Corporate/3M/US@3M-Corporate DPSeuFaeOnSCnAhaadJno.gs/LiUnugSe-bskCoeolrurt/piUooSrn-aCtoer/pMo/rUaSt@e3/M3-MC/UoSr@p3oMr-aCtoerporate AsKoiSlsu,WtwieonwecrheecdkiesdcufsosrinPgFOinSoAucolnsctenFtrriedtaiyo'ns,maendetfiornwgPeF,OwSoaunldd PkFeOAt,o hwahviechthmeaPyFuOnSdAerdocsairntga cpsoronomdbeiltePimoRnOwsiStahicostnehceefnrTtorwmaetpeihononts8o.l0ysGciiosvn.ecneWntethraaltetfotthntehbesetiadnrogtsiitnnoggomshaotilegurhtiiatolonhawlailstohwapySpuorfuof.ixcm|iaeutnntedleiyrts1ita%onndPfFthoOaSraenian1lsyx3ssiosuolfd tpohsesidbolsei,ngthseonlupteirohnaspasmpwleecboeuladnaalsykseFdretdoDesreosoisf tthoeraenailsyszgentihiecadnotsliyngmosroleutPiFoOn?S in there? If mot TSthoeckdoPsiFnOgSsAaliuntiaocnetwoanse pwraespamraeddesupfolaltow5s2:mg PFOSAI0.52 mi. acetone = 100 mg/mL. TFhiniasl wcaonscednitlurtaetdionntoof5P1F.4O8SAmi=of1m2g%/mlTween 80 to acheive the dosing solution: FFiinnaall ccoonnce oTfweaceento8n0e == "12%% ATnhdarnekwss, fgmgllnlee = lfooo00spa, -bp /s06r= Rostcone. I) + opp = lg =Fos conc. rosa} Dejee Toop/000 ppm. eGute y xlo00 "Gr7tt) [res] or(Oppo xSypbe ton =| Zoppt PMS [00 rr pp2 n x 0.03a m SmL rece = toooppl 5Tm ol0. leemn 00s = ad printed 11/04/98 NK, DE wolst \ OM Rosine) A 25m oop + page WO som = 00%2 008510 = i dilution Seve! i er erp fig ET pubs claim Jo 0 1 Orwi o 1600 db for 70S /PO 3 ald of 0st po ort st, 1 Ms of dot sop fur) 233 2 - OatsAnalyst al Tas FACT: DATA REVIEW SUMMARY FORM Toh . 1 i Data Review Sign-Off tt Na/mutee: _ FAs A FACTStudy: Toxis is Tate Sutin pertta falnhio rl, SOtuoipnAenries men: _iafps as 222 oayr Poa con'0) votoge Loo=g>efrglph hleoa: _ 7" EI xtraction Blanke_AM ers" lin ujilgn NQaumael!DAuseu.ras Review Dat Bary: Comment Sustains: QI sph, Sophy ad Pigg pds sere coil dom ce in ord~er do Lens on de aA - a n alibration Checks: __Jk = Zepph = [% devatir " ge of leas re ad low ase, _Pros cen -- ourRurge[QLpph =>Plpol oo Loge Re _oassss ExrctdBlake _A4 -- ionCrs OK Pong to 2A ov He Zogh led vaso: [oessue. < pac near ange:_[o: foppl => Fefopd oo _foctoppt [-- fssoasornchate: J= 26nd clchek = fe. lowsse-- e Wo biser Cone 2 punts. --755 Py Sows tocryoner ts woleed] in He Siok Bes! -- Tas tho, rg Dm 2d, tpl aod 152d puts sce sched icp i "rgler i fees Lew Ix 7 I ie lan 230% lov Ho forcore Curve punts. Sime omy sue hs luce 1 fle Pak lke ---- Te -- Shhgleor ecoe coepHuesh Dvidune of dre Lol and 5035s 000542 one vhles FACT: DATA REVIEW . SUMDMaAtRaRYeviFewOSRigMn-0Msti pe Ase Been, ctw Dats Analyst: Tae gers Na/mDuee: _T&s_Yi/sg [-- suet -- Man Timepoints: Tosa 2 lise confyy madresoph: Name Date: kin n/i/ag QNuaetyAmurea Retr SSavpAeminri plo frs gas Dun tar ne --oo--nop--o--ra CA YommZe utcatons: pocelE eoRange: MC 8 A s2o75n3e0 ler verge Ld, Ldn v fie tly, Arm-- MEasxiaBielein_L at4 e nes ted l] i He [smaion:--h_--e ooo lous sume au Sr oo a [cubrsion Checks: besa orasme [---- Masi Bl Fas tfulyy 2} hs, -- Tas "Ys Trp l area cg oude wreond R-ilive as 57% asd as fowas "gs -- zie fof -- _ _ - 0 |Aaatyte: cepa ine Range oe [R2: [Calibration GHecks: [------ Matrix Blank: ---- S----e or - I -- ous ee 000543 meh oo So 000544 000545 000548 000547 sr ne 1 5200 200 Tus Weiler. [ofS 000548 franc Ba, 000549 A------5 -- "; C[(CCoaoltmoapronauitonnod cr1urnBvaemo:e1:o0P.m0O3aA4A7n(xC3h+i8)57.1232213 {(RCeursipaotnyspee:tyLpien:eaErx,teOringaln:StEd,xcAlruedae, Weighting: x, Axs rans: None | 75503) || | 1 || : || |i R| esponse s | || | | ' | || | 0|0 too 2000 abo obo sobs sooo robo | 000550 [Compound 2 name: PFOS (Cha) : ! [(([SCCRoaueelrstivpbterocaitntyosipneoetn:t`cyoLfpuienDn:eeeta:Erex,r8om6Orir.nmi0aag5tilin7:o7Snd:E+,xx0cAl+.ru96ed96ae6,.903W8e52i8ghting: 1x, Axis trans: None. | | 65004 | i | : || || | | | | Respons- lo : | || 4 ; | | | | | g conc | 00 100.0 200.0 300.0 400.0 500.0 600.0 700.0 on 000551 [Compound 3 name: PFOSA (Ch3) [(CoCefafirciaennt ocfurDveet:er2m7i2n.at1i8o5n: x0.+9290732.61159 1 [[CRuersvpeonyspee:tyLpie:neaErx,teOrrnialg:StdE,xcAlruedae, Weighting: 1s, Axs trans: None 2.0805 | | =| | || 1 -- | | !| | 60 too amo abo oho | Ebo so06 7060 cone 000552 ([[[CRRCoeeorssmepppooonnruasensed:Fw4paFacnF:taomrEex2:e9Im0Sa4.l(1Ci8h.) Area 4400 :| | x | | | Response|| pEoo | : i 1 | || | | 01 92 05 0s G5 os a7 os gs 10 - LaboratoryReport Analytical Report of Data for PFOSA Pharmacokinetic Study in Rats (Sera and Liver) Laboratory Report No. FACT-TOX-145 (W2814) Requester Project No. T-71322 AttachmenEt Extraction and Analytical Data I-- 000554 Extraction Worksheet ETS-8.6.0 [MaErt eRareiicei xs Zax $44: |SRoeorrooxg. uespoam|(aporoFxo0w.5lpo|m] aprFonospom|| aoFrM50Repoxm [scual pom facual pom facial pom lacual ppm [G_aW teoSypmLoaEnD Yat | [#M0-Bcitl (sscro r:[| 8dSt. evcfssot [i#0914095531 [mrosoes vccooonrsst]]S Sol loTw ow [ [p To I] [[moRoemcsognosmtirs |w sa T 1] [[riesoossesoemreirats Tss uadg a T a T ] ] [[rpasvoearss [sSuu |vaeoww |T] T 1 [fiismosoaomoomorriraarrs [sswa [ dow wow 1 (E [revoesonn ore Ts sw [ www[ a 1] f[ipsetssmoiumCmormars SSww TL aw ] [[Rsosmmann cooonms SSuw [T TT C ola [[RReesisspt CCooonm s |SSwwL [TTT [[Reessssoresnrgres Ssww[o T T T [[RReesssseampoyrttoannggttss 1 1 Ssww|[ [ T | T [[Rresessoombrrticmoerwss |sSww [ [ TT | T] T [[sR[ oosmmanocams [ss u| u] 1] fC rommoneas 1sa7 R __-- 1 7 1 S v11| [fG~ oivmemrEixstrsacetsio-- nReto-- d__EVSE0 ~ ussuamane----TT [[PPocsiteeMTartioo:T2O5S%WvTiBASpporioporapterSemopgagteoaFTCasVoSieeviDeriT ----T eT u Ln T--e]ka E CA E T ---- TT 2. ---- _-- ---- ----YVTA C-- E--------S-- C ---- -- A ---- -- -- [oC me osee-------- --------------X 77 ----"7 7 Wi [Fierssing aSes ying wi 302omriosnstossmperva J] FukFbhoedsTSnmdteoUkVnartiiaansdeSIrwsroRegdnEe clSaspdrinTgBiSAAsLT0hf3EliagD. S SoA a ee 1012500 essen Canton ersrsoas0tome V Fom o SHComptotn s te 000555 Extraction Worksheet ETS-86.0 SaesCEBi iTsarTe[Rppoe bom [s[Rp0o5omcorm|apprFeowor|apro5p0wpaom co Box#93.200 Actual ppm [actual ppm [actual ppm [actual ppm [WKDaLyd__d_y_ |#9908e-ict] a ['---- ---- mse e e AL -- aretas ] E [rsmoosTsznsE oEimnopgastmpsng-- ----STsTwwoo -- T ------ -------- F -- ---- ------ -- T-- --T -- ---- e +tr r r [roszsomonsmpng |sot 1 TV 1 1] -- r 1 T 1------ --1 = rr r TT = ---- TT = T1711 dre TT rr I TT C r = tr ir T ---- 1 + r tr r 1 r Cr =r ] CN r aE T I e -- +------1 + J +--T1 T---- [LiverExtractionMethod_ ETS860= _-- Date&initals | | | [VPoipreersxM8a8inescs--o--ew--i-- propriateSorogateorFEEVote p agilT e 1 [Shake20min~~ Shakerspeesigeo T_T11 |Cenintuge2025min Ceninfugespeedig1 ime, ---- 111 [[RFietmeovNeit3r4ogmeinaEbvqaupaortoatfoorrtgoadncnlaeysers--------------T--em--pe--ra--te Hamm Yams TTT [[YaFoinee0xwonOsgs3e+9S1SBe gewa 307_oer e-- ssere1 ] RI, eroerssaso ion mFeaemr toliafad 200556 nS a rnne , om FR amv weavem Printed 10-129 Seo FB Se Bo EarE Ey na ria bFT roobrmiimopoincocaommm e pgaasree A a] C friEen-- inm---- 7aym------ ----M-- -- EO ------ ---- ---- -------- -- ---- brg oomsaom a Teoa T t T ] obo Tey 4+] Fromme gy I-- 2 Si -- ---------- pp ossesiene TTore 4a15y8 L T p e T --] ------] ar =r e r r e anes ------------------} ---- ost wan ei AL 0s 415 000557 mite | Pritntonnee an (ETE ETE TE[= i a a mtn pare TT, CETETET 212 Sets mcr asm pnt es EEELE iyeh ek lm rn EFEEEEE [------ asa sions Crr eTee a12 e ET EE me LE T oy osL tle -- iti 000558 DILUTIONS SUMMARY SHEET nS:tect on ue 01590 a Rieat/TiN mNal t es 2948 Aue 117/77 [ronrdsen fee WTO TT oon Toes, | frommicon fw| oboe bo | TTTT onemmT gfarwn [LL rT FT oui sev fw AT Te fw|A| gree| [| |[| bras esms ow | 71 TT ~~ |4 rrr rr rrrr Tr I---- ---- -- I ------ - r= rT I~ 2 rrr 8 vas Extraction Worksheet ET5-8-4.1 MEatopLlecso [Raorpeox.g Spoam|(aproFxCWppm|apprFoxC. Wsom|[aFpC5p0Wproom|Cammerts[Ver8ed Jewohxos_ass i [JActeualsspoim ([aecBkuckeyprodmfo[aactuaaluwpyom f[acstuaalsc3ep3psm GatSpecnan |v gst ye hae EoFSot Saonsngperi |s sw w sa ] fT [[RREESSTTOO SRgeOBm-TpveaTrz|]ssww.|~ [-- 11 ]1 [[eRESSTToOrotsopppibtaitaTz| | ssww.[woew|[ [ TT 1 T [[RReeSSTtooremoo-Tssopppeobriiaatt | | ssww| |.~[Fva ew [T [T TT]--] [[ERESSTTOOSZtoooppogbb-iiaat1Z || ssww mw sw [1T1 T || [[RReeSStTooTresDe-TSSoopoppbp-biiiaatt || ssww[ [T ~ we a[ ~ [1 w --T1 --] [[RPeSRtoOreM-TDoyooCpoenb-via| iz| ssww| [ww[fT 1 [[sSRROOSzRSzMMOOy CCoommbail sswwt {|1 [[SSRROOSGZZBMMOOyy CCoommaall |w sw p e T ] TT aga, o [SRORo aeMOym Sngka s oa u] 1] [[SSRROOszzzeerBmMOByySSmookkae ssww pT [[GSRROOz2ZzIeSBMMOOyyaSCmookmeo Ssww[| 1 1] 1 [[SSRROOSZ38MOOyyaCCoormaall sswwt | [smo OaGogol sw 11 1 oT] [Pe oossetxetwsaSreepiewispcpratsSome os PETSVamos] -- [PeeteTmol0SMTORSIOprego as SeeR Cp pee EA r ---- r--Tow -- ------ ---- --2 -- [CentrugEe2E0:8-- mr --Ca-- me ena -- a-- g -- ] -- "1 [[PRuatomneNainroegrenEvaporator VgyommeessT----o TempTereatnurgeHiozmmes Tgw,n[T|n f[FioerrvmCianegasSercGk5syangnewieh3i 0%or mfer isnsvcmargterval 0 TA 7 [pTiglaq TheToaovda-saanre honk mip ewsree fram tant ral Sum F0G@[TT vehi 101299Ora AtachmentA ExtaEcorfiPsRoOanSrfromSerum VFeorrtmCeosomypJlUaLin10]14 ad 000560 Extraction Worksheet ETS.8-4.1 ( focmeoaSiT acieio msv s|e rem ongagaf Soomm||Fa0P0cCxH 0a3] :a[soF ectootmmCa|is: neF nSopoam CaanLveea Sy [SROSZIMDyaCovel |~ we| ge [SRO3ZSOMOyaSmohg | swt |TL [Roa mOyesmoRo |SutTT [oRo3zsaMOyasmokg |sur | [RO32ZMOyasmghg | suc| [TT [Roazsamoyasmohg 1 Sut 1 1 1 TTT te ee es eit | Ti mr ZrTT PSSS O N ASS rr =TTT rr Ar TTT ee ee te LT] ee otloi Yrymemr] rr TTTTT -------- SO WS SYSU----Sui -- _-- [eeem-- ee r | ---------- ee e e TT TTT rr rrr |I NSSO T-- STSS". S-- ee Tr A ei ee[eerie {remem | eee {ee emm--Soe freee] HZ ee ET PP Ieee eeee i] T PV eed] er ere pr----ae terme erate pryen] et 11 11 [BlankRobb t Seco SWEddceade emonnt 0a [1 [WPeipneetxeiM8a8te_cspiewih Sppropratesurogateor FCM Voume_Z0m----age 1n [PipetteTmiofOSMTBApHTO pH Lo leSO Jig 1 TT ] TT [DispenseSmiofmethybtbubyTether [Shake20min _____ -- TNARide Shakerspeed Zoo TT 1 I ante |Addmethanol"VoumeTw_----TNAgel Es a a es [\FWiofrertevxi3n0gse3 c3--c --5-0--sy--nn--ge-- wit a 0.2umther mioanswosamplerval 11 111 1 | ~--] 10125 oatAracament A CxvactinEnFsOeSnfom Som vfomeCosJmpLtaonLeoffrt 000561 EKroomSs ESre8 enm Trem, aHox1ota Sample WeighsVolumes Snet:ymemoerTox15 -- Ai nm EeLn -------- onnaes vy CCoomneorr oRreorsz2s8i4noDyy1CCoomne! porveanms msmggttes 1 a tm Ry I I 1 rrr | T rare y smer ---- I -- I I I I -- --7]-- Cr---- ---- ---- ---- fRoRkooComsomneoyysrCooltnes | rr ] rr pp[rr9Roa0;3s2ss5i1sMee.pDmyyp4i,ysmemg/sgketg.sgs [ [| | | provssaomsmns | LL J T ep T -- -- pe -- TeeR| p-- tr----__--m-- Ee, we ange [1 000562 ig on oeSted Coven Ft Ses ---- > ETET EEEE CE 1 (ETE = CRETETm] I 003 ow CCEETTEETTETTETEE8 TemEIgRe ~w~o i. sam0)p 000563 DILUTIONS SUMMARY SHEET DBoiut:n9D93u06an, MC, 0313f9r EMiatcroaTnimebpoaiavtiRatSeSrEaDay0s8918545. [eer e or r rr e Tee Tern frase sora Joo | | ge fresno mga oo| prose sopraJoo | | |TT Prose smgaeoo| Prowse asmpreJoo| pronssonssmpty fou| |FT TT froinasm Joo| | TT fronseneom smprdoe| | frossemomusendon| | TV ft 1 tT I cl [rrTT ----[-- rTTT 4 TT 11 rT 1 3 f-- 1 |1 1 22 oO NOL R iyprL |TTT ----"1 2 veFcaemsCSomHptLon_0/f7 Extariion Worksheet ETS.8:6.0 OG Kobo= 1/05/55 Sowae:yZZF7272F 77527 [srwooe sSoam |s1oFxCTsom, rFeC&cWsaem| appFsE3V0pm Comers Vered 57 [a Box #56 -- ZL |c[umlSospe om |p [:scuaolippeuomssscBuoadltie4yp-omH2 scantp)pom BimGaaegspmreeodwin=ars |" sousui c olperT s iE lo Lrc7h|i|a| t| l Co esereeTsosouw |z2 ou 1 ZTFT Cw meer TT5s6o0u0 wswu [pZ g F7 T1 TT] 1 ClemmowerTsoSouu wmwwLr7 T7p Tf 17] ewwerrT5f0ou01-- 7A wwua[ [7A TTT] [mowe owt[|Ssoouw[ 7 7[wa [Z wwTTT] K-- ----rrprp 1 -- ~~ --rrr r r ~~ ~~ r r r 7 r 7 ~-- e -- r -- T H r r r r 1 ~~ 1 F1 oiTTor So JhoririT s wemelep mpd gm] TT NowceSee---- Jah gev 1] [e[BtspeonsmeEnmo--lm--emo rarer SweTggx gT TPT IT EE ---------- ---- [F[ utonNivogenEvm apormorioionee sspwTempr eroA LMegmlaa me s fr eTe TT_TT] o "Cont Cal Varfcaustedoanmse mas 33T To 2 ure e T --Z-- | 101299Ora Arachment A Zarsceo1fisF8oO5nS0 rom Ler FVoemrCa8ipyltSeetduon 10/25/11 0080565 fu 1h ede Sa ERE = EEE oman ---- atria SHEEEeEE EEEEE [==[5 od pm He mene Tem mTmEmE---- Ce mn it se ae TeTe TT 8Ta ie SH ast {e556 Extraction Worksheet ETS-8-4.1 Ey a ISatane_Z ET S227 [FRromoangsbSom|[36F61C0W6po0m5]aporFosHom|| apFpC0rTpooxm [lworaer 7 2c7 lee 2s60 7so0n21f=o.Bcoaalt p4om0l|avcB.iea"v3o son|al" Pop-som Vera Ristzzagmseoemm-T| "ssoouu-- 4 gn-- A an [Tse m Tssoouo 1mWew T 4 TV T TT] T] Cme er s5o00oTgf p s ewupTTT1] Temem, o T[ssoouw 1{ 0 T Tu a 3 sdTTT TT] ] [msmer ssoosoTT 1 Tw ouT1 Ja T] T | r rr r rr 1 ry 1 T rS ee 11] r~ r --T r rrg ~ - Ee o TIr FSot -- Sor r ZodZiT g senL za11 } --------] 1--|] aE fanedFEgaT owtmms [PEigEon ars.Soewr popes oreoegaeeFe C eVm ou ee ek mEAf ESE ToTem] fee] ([RPaotsmonenNiavnogsenEvaporatortoVaoynteses LanTempeTrNaRte_H2a7m7Tyams (WFColornrteurCssiaongmV+aecSeea-- ctiBo0nss uy-- sesdswamis-- sm0a2isi3T&rr2eEnvesvemsrgerv oisJ --pmr TT T] |] 101129Ora Aschment A fon EofrPsEaSs ram Serum VFeormnCBtoymspelaexdt.ion 1o/as/sT 000557 WE" ga holo me Coe x vim 130 1 ral ot tot eE n -- r rer r Srstrmreli: Co TE TETETETETEImi, ees Steels 200568 vrdues: rif49 SE Terns Target abyss Pas [Poaq. smTogs i EE E e mm E e _-- meZee Madris ks Semnles see spind wh ZT5TH pk wad [2Eud otbl, pr io Ae Smale - - a. 000569 Erren Se ae [ Si rl dos <Hh-2h] - 2eatp oraenr als y.d Dilains were dome 12 nt 75 |Zhasa, and Porn lnts fe range of /ksona 4 sl 5 Kae ages foctn. (asecdid sts HeimatiTua l follu owinedicfit fon --_--d -- Poe/PeAs 000 Emme Ta + 0004 - Z = en m 71 Vile ins[opt of 00 phon dlikelto Jonette -- Tis Dove pay Corvecbed bee <0/00! spies --,-- [ Zasnpdl4m ae moize EmporEsrcionti is Peto 000570 rasan i Sim re Bln Sample ExtractionShee ym arts er IFogh Gia of Le Sato und] 2% pupacad fic fanal SIDfds sd ie onde dls Fo cone IETo Eijms but do shor4 leitglam Tras Prey 7 at = Yn _ ap Rp ESA pe E-- lan:Ta EEA ]--F --, ee e eee p -- 3 000571 Extraction Worksheet ETS.8.4.1 a aTaEmnaran Riorhmeoog0p0o5S5 T(s5o1F0c<t0C.p9om]oc5x3T5s0omr[| arepressw0cppmm eT [\otEiDamyCOosaSyo2ea0ummnanJ n |ipre1704) S ab al U | rv> T saaslt oTffr msrror omsaomimaasms-- Ts--u usrur ---- ----e ------ -- [fo[eivnmomsmmernaaetee----T----s$suuwTT ooww T} ----] e ----T TS ---------- rr------ TTTT -- E ------E ------ ---- i ---- -- oef[mrse= mmoonmaan-- sT I --su -- s ---- u p-- Taw ---- ] ---- . -- C[[rro oosmemmeoioonns-- ns T-- susTuT ------------------{{|| e [PT rreoe mmeimnas e s sw u T ] PrrosoTmeemman-- rseonynT --s-- u s ----u ------ --] [Blank Rabbi Ses---- Siowapy 3-Gy_ smewieibm ---------- | [Fomexi650 | [[PPiopsettteeTMaTinors0piSkMeTwiBhAappp1ro0pripatreseurrogatTeoirrFeCM VowSmeePLOR--EmT---- |L ] [Fost Zim oT025 Na,COJ0ZSMRaHCD, buter-- Sa30A0Eg = DTT] [Qispense 3molmethyit bubTether TWA JpTTT] [[PRoetmoonvNeiSro4gmenaElvaaupaotraotroorgtao dnrcyanyeessr--------Tempers HammTame 1 ] 5] [Addmethanol VolumeTO TRA PET] [vs CFoonererxo3Ca0sseeSc_ ear_n_--gS--oem-- esR-- ETom mBforSE ] FPIHT2ThAe MBShSOeIaandMSaei1 sScitDes s3asHtaenkrsnwefriekE renaodR mnc,omlRecRohtem TaaTCseRaOLmGpIAv"a5d0aeotwwceofmplosetely ei 10125 ort vacnment Extacion e1rPsEeOSstomSerum VaFraimesGooympgiisisn noos72 fE remn. Pres Osea: 110390 i aa) } EEC T StudyNumber: me TOXS etna po 1 | E CcE amaE s EEE -- oC I = A LE cei HEHEHE . ji wn | ws us IE] EEE (ETETEETER ie LSSa [== 5xTer[a Emmy [=e 2) THC - sIeN nts aman ++ sic oan srs CeToTETae a fu heJ ol,J 000573 DILUTIONS SUMMARY SHEET SBEeLlTON am ci il i pram el [re rrr fronscsnprpon |ET PO os dr i 0 fronssmppgomn |ovo | | | |] fresstsmrgonn| 00 | || | pros-- sssmpgomn |F 0| [1 | 11| TT =1 Tt Te 1 = e 1 T1 t Tw r = T T T Jr T Trt TTT] TT . TTT Tr TT] (-- TT rrrr 3 bl 1] q error Tv | J mee (1 Orewa id-2hy 15 fn Extraction Worksheet ET5.8:6.0 ftoSsnB os IaEtE e ce[okwroeogpSmTm (39s9c[08R0o5ws5mo] oern som |OselmreeoSnworom-ns som on 22 foseS woo m BE m [aS Te me See SeSo Riza JCn ovi]sslefel7ils li e-la|fbt] PWEaZivasgereioremsse T[s--ssosooouuuw---- --g1~r--1 --1qe--uu --------A--1 -- 1-- ---- J-- lueA /g E Es mmmm eeere reeTTs --sssooouauT--o --aeowuT --a ---- u F u ] +] smmoemmreas Tsssooouau meer sou A rouo ------ ] b[a Se oome mrssowg ed aSpe 89 t Uasoresrae] r --r --rr -- r--1 F ] -- V 1 t = - r r 1 r= e r tm 1 r esetf E E A -- -- S E -- ---- --(---- f [Pree aeteeMTaTtoGsTSe OpSeWTSBFRShppr1op0raplerseurogate 0 FCT SVolStmae 7S gi grt mP o eT r] e EspE ares oTmen --an--T--se--r Te r-- _S F3gp ------ pe] [(aPEmdoomnreMeia8onTgsTeanTEGvaUpSoraStToorrtgoaVdnoiylcnBueymseerJ7T.oT E mpers5 tire 7 7Ra7aF Ta ege ]f NS {o eeu e| pe (eCronaCno V3e5reheaBt0onsoyneeSwaimne3m02tumaferoioanswampler va] 101289010 Atscrment a ExvacteornosFaOsSofam ie VFaemrGotmepLeLn fos 000575 J SRMmtrihan.,eha.i,e. Sample Weights Volumes Corp Fatrios pr a SPoairsaemar abri :MQWatt er Teer e|se]] [o[ m insmm moen ea 1{ a2o gn /y0m-- [s sat-- as wt ] onFZoLr omsmmmimGa50sea] meee7[de|o2l t 1EE r I -- -- rr I --I 1 EO -- 1-- 7 5 1 e -- -- = f =r r os | r r ct Er r YES -- -- a-- : -- 1EE -- =-- Sr ReviewOne [oe [-- "Veet hteuff cNOs7E sen phi tan a Enmay om roe a an Prvpnrt wily ining may Meow Sr et pt res hc. oT a a iysom mots 30 ww iii = I 3 | BEE MN otvee 310 io natin=~ g LeToeT3313 [-- woidd by { ar fed 000577 DILUTIONS SUMMARY SHEET BSoDtruidoyn:9nT9O2DX1t.e11A4n5sbt: SAL 11999 `MESaxoitrheaeacnTitioTmnNeDpNaoatibote:arn:aaMtyLesORHiWDTvsWAy .2t191,343G98r16p, f ro ee fromssatonmsegy [ot00|--------F--1F p-- lito> p] brossmmasesns| | A T 11r = f rr rrr rr--r ] ra gH r I re r rr ] 2 -- I = =rEE EN SS EE I I SE EE I -- ---- 3aS F = r rT a fe0--00rdstUst ing 1021 ] Sompliand AGT JatHT FACT: DATAREVIEW SUMMARY FORM ~~ 7%/oy p 1iz - . 3 DataReview Sign-0r | Dunn Tes Niate oueris fy MeTswye Bol nes Elson Dy Hd. pol elf sr VTechnnRevSE flog Seip SeayAnis QNuaoyAemareRein Ouch eet tials any Sup orm ner 1AC. 10]21]%9 Comms ato: Tae|SR or. _Zutn (cn 7 hoe ks aa a EncinBacc_i MaiBasi o ew iti le > a Epp ecg Janae: is cones \coeptableLinearRange: 2pl. 2 Wd 0022p sence fdr bt | a? [oozuon ceosed02eZig fwonswtBask=fosHanLow I roses Dhders soda Sc dig grind, be | locoopa igs 5Ca1 >vi3knp od gon | be omy eve os a127 ninote 21 al = Ih loenss ok 4 oh wuhei te ach blade belly He did Sengle Hoy of Hestple had Ligh arm coule and eel outer turdol hlful ot tOezcinFeuopflgaududdSeedlin pailisslet treedp omtle cond ener tonion ay BA ootA si-puificS ant, " Quando ntactsucsute3\otd piguesd oc4d nesusa focsicanalogn) fB@ olco, t8 oratopnA Ke un" ndiGll_ uotoeldaQs_ ives i 000579 FACT: DATA REVIEW SUMMARY FORM Zo< ff, 1.1, p : -" N >. ---- DataReview Sign-Ofr Duwi gms * Analyst Review NewDwe_Zmenff Meacatswiys:: TBalwow 1d od Tile TNeeaer ru gu ogy 1a) club Seeie pe sep niin Quitioyhmmmoaieer _ Duis met fpf doy aan emetcllutd Come dutcrtons -ezw-p -- D Til Hi pranks lok Losishod Mp5 ls f; ol hoo sn oma_r| 22 SE ee Ox__w ---- Data Status os: cpl Lio Rage: og [2 Shion Checks: re Joesae = ExtractedBip - Marx,e-- (as 1 Vi 3, KY eugh crc ble Ecutps MaeBak _ --_-- | I 000580 Suit comentpuma SS ws Wok burgh one abe od wd. oo 1o/a)gg 000581 - - -- name EE SHINEE EE OEE GE OBER OBI Bie SEE se on. Somes ena vm im HE i (Lee Aube LE EEeenuae EOE ean 000582 E---------- mom PE EEMREERE am mE hd 000583 EE py UC 1o/aifeq BEST COPY AVAILABLE 000584 RCF(GoeaonorsosueieamrrE1r3Fo0OR8A3,(1A)va os . | . | i x ason .3 ve | | Ne------------ 00 01 02 03 04 05 06 07. 08 09 Eros 000585 pfoicuinendt o2fnDaetmeer:miPnRatOiSon(:C0H.a9)88440 [Raelsibproantsioentcyuprev:eE:x2t2e.rn5a6l89Si*d,xA+re1a38.989 ure type: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: Noe 222 dn bet * J I 1 00 e 2000 4000. r 6000. 800. Gor 000586 [CoC omaphaaaona ndicoBuroa:rut er:0m0i3nO7aa 1SanA:GsoHgn 8)s1e020411 BCaotrsoenroCeoata, Orrg:iSa hAore,a Weighing s,As ran:None 1s J Respons | err 00 2000 4000 6000 8000 Some 000587 E Brenere r a ee etea . . !| x. ' | . | 1 | : 1 rapa!1 | Vo ST SOT ATYS ST 2 Sr , 000588 : BEST COPY AVAILABLE ------ EH am ot Sop cium aclilen 000589 DED LUNY AVAILADLE from ow BEST COPY AvaiLagLg 000590 BEST COPY AVAILABLE = fe 2 2A, Sup its gas fuler 000591 BES| CUPY AVAILABLE --__------ en Spears meds 000592 27955 Quo,Kae : `SOUP 020199: FACT Equipment Log 99092 PAGE 11 DATE_10/19/99 ASTNUADLYNYUSMTB.ER.TOXL45 MATRIX. DESCRIPTDIayOsLN FACTMethod: ETS:8:5.1 neonvolume: 10uL Cyclede10min, ElcwodeZone MobicPhe waomMMCHC GMen whi \ TNAmidmobe Prod ae) (0) RSIOM | WGPomh GINA GLE Epis wom ome Sorcumomiens? Bo N Over. Gurdon Noe Parsuaber. Lot mein Auiytclcoum:Keys Bea Sue2200m TREC Spheresie:um Partnumber 0557012 LotmmberBIEL SelaberQs214062 Anitaprsters: Dringgs(UN)250__ EST eblgis(iALnLLg ShguseLBth i LCFlowrie0d, Back resG5b:ac HPLC solvent grad10mein1s0.;95 T noow-- ew Jes g me we nag oe ase me asm we 000593 BEST COPY AVAILABLE ning Method Report . Methods [I ---- srinsaa ue oct 18 13040103 199 FRR i I Eiara:fige5i5e](30 58 079% rae 1 | =Hee se Rte BY I |I Eh (8 RR Ls some xs) Sacaanpcialtlnoarry [freestored ser nam W F3O 00 k3.0 E$3E52 1m f-- Analyser vacuum Gas cot nsx 50s Jv a xio rraeesseotlnuuctiioonn Lionntaimpeireary mam a i1laRreatssooetluucsiioonn fSTooonnnateretnyy ramp Smsse see na oi10f0 b0oos s [. 1100.0 . 5F2i8 a Go 000594 --Ee------------ o0s% 000595 Massiyns-SampleLit `PSreomtpelce TsotoOCtA19M1A6:4S23S6o1Lu69pY8uNt poSampleDBHO1908.L Paget PagePostion: 1,1) Flotama FlaText usFie 21 111000119000090000111423MMiMaeecoOoHbBBaaannnkkk 3 i0iom0rs TOXI4EABSI01Bi9n9k-12SE0E 111000MMMIINNPPPEEFOOOSSSPPPOOOARAPAEPPOEEOSOSASAAI11SSS 10MINPEOSPOA PEOSTAS 7831011I00001130R008O01l18e7 TTTOOOXXXI4IIEA4AGETSAAISSTBoOerSSNaaBBNIIllDaaOnO.kkk1NSN111oSGSSSrEEESOdEEE 3 101300018 TOXIASABOI0NODsop:SEE 1I100OMMMINIINNPPPEEFOOOSSSPPPOOOARAAAPPPFFOOOSSSA1IAASS8 10MINPEOSPOA PFOSAS I1300 111000111803990000000832120TTOTKOOIXSKRIIG4140sS1RGRBSEt2sS6o1o0pI0po0mpOi1n1Nt1:.,6GSSSE0EEDEEE 12101000025 TOX14sRB1S00gI01I1,ESESE II10MOOIN PMUEOIIS PPFNNOOORARPPANPPFFPFFFOOOOOSSAASSSLL1ASSS 10MINPFOSPOA PFOSAIS .4 11133310111000119099090000322548 TTTOOuOIu4KS1RIBdSSIs0RIREBE0Ss72S08801001000000000001111111,:.00SSSE86EEEEE 16 101990027 TOKI4SRBS10166-10010,mSmEeE I1I0OMM0IINNPMPFEOOISSPPPNOOORAPRAPPPFEEFOOOOSSSAAAIS1SS1S TOMINPFOSPOA PFOSAIS 111987101[10001199000090000332809 20 101300001 MTTOeXOIHO4BSaAnIXeS S 0I220N A00,DS OUO yT,.CCFoonR nvT11T O01NSGOBB, GD,SSEEEE TOXSRVOIEUAM.DSy.CAonkTINS ARO, SEE 11I0MOOINMPMFIOISNPPP-OOO.AAAPRPPPPFFFEOOOOSSOSAASIISA1SSS | MISPPORFN POFOSA. 221 111000111899090000000000:02 TT TOOXNIO ASSRATGXS!1S0S1I FR9OsOSSE3SSUAoM,m,DOpSlYb.,ECCCAooVnnv-ioIrTlSTII-ENN,IESAWRRIO.SSEEEE 51101690035 oondank |ITOWWMIIINNPPPFEFOOOSSSPPPOOOARRKKPPPFFFOOOSSASAL1ASSS IOUIPNOPR PFEOOSASLS Z32T5101119009119909909900030708 2 101999009 T T TO O OK K XSSSI RRI IROOOIIJSSIA A AEETMMM.,,OODS S Sydei,,GCCA A A ooonnttnrrooT oT TllIIIWGGS IS S NIAAGRRROOO, . ,SSSEEEEEE TOKSRIOL3A EM,DSokCAorT oIWS IWRO.SEE 1M0MIOINNPMPFFOOISS..PBBNOOOARAPPAREPFOPEEOSOOSSAAA|S|SSS... 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S Oy.SAMokTa10S 199, SEE 11T00MMOIINNUPPFEIOOSSNPPPOOO-ARAPKPPPFFEFOOOOSSASASL1A1S88 1OUINPO-APXPFFOOSASLS . 4@5 G 11100011199900000000555243 T TTOXWO OSATSX X,SSSRRI IOOSNRISS2U UOMOU,.DODS SSyYW..,2A RmMmoooSkRkT TaQG111I00011S S 1090N88000, , SSS.EEEEEE 101830085 TOXIAS2R50B090SCO0V-111a5.S0EE 111OOOUUUIIINNPPPNOO--OAPAXXPPPFPPFFFFFOOOOOOSSSAAASISSLLSSS TOMINPEOSPOA PFOSIAS W&4BI01111800091119030005090000085550078 MTT TaOOdKXIIO SSSRAaBTnSSkX1,0S1SFR9I0O533F2e555A c44-o21p1Mb,,-OSDOyEyVe-SR 1m1m-oo,kThSyE11E00S1190B99, SSECEE.. 1TI1O0NMMOIINNPPPMFEEOOOISSSPPPFNOOOOAAARPAPPEPPFFFFOOOOOSSSSAAAASILL1SSSS 581 111100001111888699390300000000661052 MTTToOOOOK4XKdsaRIInEkS4S1SA0BTSS1oaSSrra91IBBBa9aa00nnnk11Ikk11,.,20ESSS0EE5SEEEE JTTOOOMNMIII PPPFEEOOSSSPPPOOOAAARPPPFFFOOOSSSAAAIIISSS IOMIPNORPNPFFOOSASLS 55501011110000911100009990900060884875TOTTTXOOIO4KKSKIRIBSSII0RRISEEERASSSS2tI0opITo0roo0apn0moS1BI11]lI,1,aGS.INnESSEkEE0DE.EESIEE TIIOMMOII PPMFEOOISS PPPNOOORRPAANPPFPFEEOOOOSSSAAASLSSS. IOMIPNORPNPEFOOSASS 55 11100011100090990000887980 TTTOXOOI4uKE1IA4BSS8RIROOOI1s1SSo0ps00Ip2mo0I51110I11E,,0SESSD0EEEE0EEE TTTOOMMOII PPMFFOOISS.PPNPOOORAPRARKPPPFFFFOOOOSSSAAASLISSS Mass Sample Ln F Pred Toa Oct1916p :41293960 m---------- WetFle BotteinactVolume SampleType Gone op1ap mitoeamentrs 10 BBICz === 8fipr louilemmiaesiie-eBi3 BBBzTz =zs 3 pplirimicaiseoesm & BBB=zz ==z f1Ho ilmmoimeaeisoss 3 3 7 Bomai h ppBi zi pi a=2= : 1i ji m mmineee itnss 2i41 BBBji zzz ==s= IJH onemitmsei BBBzzz z:= a if8oilro lmsmete aRsoE i 8 8 BBz=Z =z=5 Pi Booommaeen 28 2BBszo z== fBLioemmteaesn)22 BRTzZ :z= $53ffi lloenmmteeeenenEs 28 BBjBi ozT =z=: B$llom meoinom a23 BBBozz z== fo8 oellmteeenR]88 BBBooz === Y 8488 oelmemeemenss 2 82 BBBBzIzo z>-= oleleonmminiesens u 3 BBBzzz >s: 8omdleeenmineiE B8| RBBxzz -== 888 mmiiemeissn 3 hp2E i2 --= 88 m iiooi nss pR3iC -==z i8# mRmeNiiEe 1 ww% zz z=z S75 ot PadsFohn lity Ma-- sa SameList. _---- zPooFd Bn Pi_na--Tom 18 152421e 95080 r Pageposta 1.) = Flavus FeTast B o101o600r075 TTTOOcOuXsn1Bs4to5rAesBseoSonoeoIssO,NsSSGeEEEE sre T1IoNuOnEeRpUrOoSNsPpPoOPOMNpFFrFoFIOsOOnBSSINSSS 000598 -- esa sampleLat _---- Y7 Poarn9t 5 SFe ainlTUono:CoAuMoASSrLe YeNaTots rOSar TIOBOISE SPLstPe agepostin.) IetFle sot injectVoume SampleType Cane @5a tlibommmaesisk uiw 0 BRz= -==z 000599 FACT: DATA REVIEW SUMMARY FORM fog 162 one hth . Du Revie Sig Ot WUaeys [a Neo aethefy acres Te 1s Montane. Bde Dlidis WI, od Til Newt ie salostey Stic posag, soaparia QNuiteyAomareRen Sechrest Tas ovatorr Nd colind nud veuadg Ue wma _ Ante sun Commas Stcsons: kite [-- Powcm? OLsieto cit-econnite ot cope iocang: HA I hoes penne se to uedelmnor duce Conds end bes ue I ebukin hrs and cee dehy -- TE| shld pod he weed Me [omamenntf e ------[ -- A e-o pled dle Pak chide Puke alot] ke sacshipeld Tes asthy Iasi: Pres causy @ Cllhiten chk Be pos =r ile Ttio acpechd fat hoo rsp [a Yelm. 4 = peli Iva _0.9p5205 Ms snk gry os 107 4 aion Chocks _Failed { hesaso_ esd Yo real Ac 10aslat diped # fous sue v 4 4 : our: _Prsss ceadsD dhoaiitccaone punink k bbh oDA uoro tleessw ! er dasa Ivss ed fo Osim a a er nde, [rz O si_0. nh.H id ss an. dalog TiJingoosut ddoiLfH ue prone loud do woody aus ioladlag pile [eo] 47 3D or pare bods oe Hr pode 1n Aa. lowsge dons Aor hie punk snAe (L) Susarsr rede oe Tneerin savas ADTE RA Awnirsss OF TEgpSlama Sog E dongle AEeec1t95+s/93wag, md podfom Ad cure 0 tha Sonkaing cabbishn 000600 FACT: DATAREVIEW SUMMARY FORM faye 2002 IS Cepuleontopets one own Let Zar FsAtCeTiSntudy #: BToSrreWiha sie [Br Sonne trFn it . 3, lrg eeDataReviewSign-Off No pended toell eeehr Rul sswennoin puE nto lak ooor waddo rracalynsuc 5 fre te | ---- Glhss zerfl Pepa =[ohdeonChocks HA -- Maku a oc on Cote [-- ste LAT 2hZ ol Eildia elcesive Ilfd, & | uchodd-dueheles Onerespush [ub cinta | in He cones Id ea tice yg EE [roe go ote ExncedBunk pa Dia Satis: Ea OSs lod be coven. dSeemple oHveebfurl]eeLdycalhoekkceaTrv]ees. I r tonnests @ LE ds toeAotghd cofoleck lo cenlh a te tudebl0da .meth | | 000601 Taerfuly_p Jebd ii BED iE SERRE iB OEE BE 3 EE OEE OH 4 000602 000603 Lo ~00604 000605 i-- FRCeepemomseeebFrtomeEorag. Ara ' i | || Resp | . Tas.sefecfor 2 My : { Le 000606 BEST COPY AVAILABLE sna f lReistproantseonyecr "umExtEer8n8al5S3em a, Are-- s108462 EX m| ea: Gain Exe, Weighting: 1s,Axis ras None oo - Sin i i -- / / . . : / / | opnx) Tho wo mo wos comet 000607 BEST COPY AVAwnw== DED) WWI 3 Mave vee vee SRE oo = E CC e oO eE hsiraiWiseeisghing: Us,Aan: None Tre : o . - - | | | | . / J |e So we BEST copy AVAILABLE 000608 BEST COPY AVAILABLE * EE et ae ane eran me ee | J oa 000609 ram mann om Fac ulrs p44 fede oiovte Zan ie ga i Be 000610 eee eee a mano EE FEL Em wa gem. BE imam. Ee Ems Be fd . _ 000611 rr Lo Te gee, BE EERE He 000612 ------------------ EE------ ots 000613 Fea hoeley QAMuELaIItA.s0ro6424o4s9E8: FACT Equipment Log 99114 PAGE DATE. 102209 ANALYST0. STUDYNUMBER:TOXI45 MATRIX: Ratsers DESCRIDPayT16I4dOiuNio:ns FACT Meo: ETS:51 Injection volume: I0WL__ Cycletie: 10m, Bled: ZSpry MobilePhase: (WpomMNmCHICO? (BMeoH T orsoA duber (A) S08810-14 roe RZ Prep (ely) (A)101650sb 1020590 Exper wou ou Sor umomumg? { or N Ober Guardcoum: Nose Panter Lot ounberzs Analytica coma: KeystoneBeas Sar 2xs0mm peice Pansume 0557012 LotmumberPOEI1_ Assy parameters: Drgays(Ubie) 3n00g ESTnebulgais(LzUAn)1g0 LCFlowru: 300amin Backres 2b HPLCsolventgradi0em n1t09:5 Trirme ain f"oao 2100 100 9550 950 9150 350 80 100 Spheresar: Sum Serlsmber0S1BI6Z Shgaes(aLh)t0_h__ 2008514 BEST COPY AVAILABLE eSarnieng aesh--od --Raproz--------reee- eeerste [I Yatra: CAS SL\NHELIACRATS. ROAAGEEDBNPROS1-6K princes Fei ous 22 08ise:58 1999 Ba ii oo . | MerE EA E u NE Ee o CE lRT SRE [E-- ccaSoinptdtaicieaerry BEGoanmeer te. se nan LL3eaoonnLte B30 100 or iiTinoonneReSBsseoeetrhruai)tyeiioemnname fon rier su nan 11385.0 eboo, Peasmren na smaiyses acum 120m Gas cons J az 1SiRdesnontusdion oifrootnntBserEny name fn rier se nan 11B00a 2BY ow Fpi n 000615 000616 ie a -wy Natnod Report NeaitthodedTiitlieesa Priated: Page 1 cFrr\imaass,alybmexv\oabmeerli33a,-ca1t9s95pr0o3\1a3c6q1u3a6b\10mta-10-55 Friday, October 22, 1999 09:89:35 P1100 LC Pump Taitial Conditions Sotvents a 0.00 o ois HSPitlnoopwpTr(iemmse/s.muir(nen)in(ea)r) 10o0.0300 SOVauvxeennPTTreeemmsppsueerrreaattuu(rrbeearR)Liogthet((C+C)). 013d00.0 P1100 1C Pump Gradient Timetable The gradient Timatable contains 5 entries which are : Ti17m10e300 35a0000 i1o0al00 oSho8 oooli 0o.31w30000 Preopsotsure a7555300 535:2.]5 8[5380d5 3aai'o s8oi:is8 58911:3330000 oioo 1100 16 Pump Reterasl Brest Timetable he Timetable contains 3 entries which are iTitn0ee50 ColSumeSeetSwitch CoonStneatct CooSnnttaect2 Coonoeteactd Cont&Saecfts 830 Si Sie Ste St Sie P1100 Autonampler aitial Conditions BEDjrieaacwepspoSespeeiedtdionGud(/mmm)in) 2200009..000 VSniteaoclptNTiioomrenb.eVr(omliunmee) (sl) B1501050 000617 Matatyns -Sample Uist SFaammpeleLFistrcCAMASSSsUeYNOCoammeta ts PROYSA0B1022008.SPL Pe PagePoston: s1,d Floame FlaTest 131 11i0o20z2m90o9o0sn00sMMeTeOcOUkHBB4llBaanRkkBSIONGIHOBNSIEZE i 11iG00z22z99s00o00o00n48s TTTOKOOI4XSURMSBSRRBSTSSSSIeoSIovn0enImOn1Ba0INSk1Ei0102E20.SS00EEEE 733 iiG0CBozszezsooooors TTTOOOAISxdRISBSR4IBSOSIRIGBOtIISoGNIoSnTO0ts0Na0SSSG1eEES2eEE 11100022222000000111:10 TTTOOXOXIUdXRESRIBBSAIOGSINRBI2EB1800RS0O090II0011I0E72.SISSOEEEEEEE T1i5i1011e002E02z9El0m00s1d5 aTTOOXHIXAcRa1BrSAeISONRNROT10S8000IPR1OD21N2SSESEREE 111887 1110002222590090000111e87 TTTOOOKIUXSTRITSSOASnR0OS0S224RoSUMI,:DDDSaooyv.O,.SSrmRmoophkOhis.sDDDDSSSOOOZ-awexeAx1IioIQTNNHaEAHnmRsgD 3B 15 E1I002E2E3E0O0I2S0 TTTOOXOKIAESAXRRTaISSsSIiSRmROAs SOEAGGMM,SDe oAylBSm mMi ok.SypDeDDSSOOaehxyIIOOiNH,WA.RRSa 123 101200022900057s23 TMTOeOXOIUH4sSSaRRnTTkSSSIRaRSOIEOIMN.,DDaarykdSSmmookkey,.DDSIOO-IxtNQN1ESSS 3278 1003203am3%0000rs2s7eTTOOTKXIUO4SSRRRKTTTSSSOSIIRRIOnGI4S2iE5I4aNS,IMD.wDDeayyyddaS,,SSrmvoOikResGD,,SDDo0aobS..IT1OW0II1O6AR1NDG 35S0o 11I00C222E000002R30 TTOaXOoVrNeSSSRiRnBa.SIs01eissCohoioBOieNz2ST0EE SEE 3B51i1021l20002200e00n0 TOTTXOIOAXSXRMT\SE4IORSI BRSSSSooBneInuoSmm0bzbIiIKS01iEEZzEISSEEEEE 53311002l32o0a00n00s0 TTTOOXOX\SAXSRRIBBSASIIOEOIIRmSt2ABo0RoS0Io1I1Z7zOSSSEIEEEEE 333 1011000222900000330 TT1OOXXXI\14E5SRRRBBBSSSIII0001I00S2s10800000000000.111322.SSSEEEEEE D 1I0C0R TTEXRaISOIiOEamE10R0001B212S.SESEEE sre 1II0OOMMMIIINNNPPPFFPFOOOOSSAPPROOIAAASII-SSPPPEFFOOOSSSAAA 1II0OOMMUIINNPPPEEFOOOSSSPPPOOORRMINSSPPPFFFOOOSSSAAA 1II0OMMIOINNPPUFEOOSSNPPPOAOPRAINIE1SSSPPOPFEFOOOSSSSAAA IOIOMOMIUINNPPPFFFOOOSSSPPPOOORRANMIISSSPPPFFFOOOSSSAAA ITTOOUDUNNPPMFEOOISSPPPNOOOAPXMMIFSSS PPPOFFFOOOSSSSAAA IITOOMMUIINPPPFFFOOPOSSSOPPMAOIANSRIPSSFPPOEEOOSSSAAA IIMOIIMNNPOPFFUOOSSIPPPOO-ORANMRPIISSSFPPPFOFFOOOSSSSAAA TIIOOOUMIMINNN-PPPFEFOOOSSSPPPOOOAARRMIISSSPPPFFFOOOSSSAAA II1OOMOMINN-UPPFFOIOSSNPPPOO-AOARA.APRIISSSFPPPEFFOOOOSSSSAAA TTTOOMMIONPPMFFOOSSNPPPOOOPAAARIEIISSSPPPOFFFOOOSSSAAA OIIMIOOPMMFOSNNPPPOOOPPAAA FEIIISSSPPOOPFFEOOOSSSSSAAA 1II0OOMMMINNNPPPFFFOOOSSSPPPOOORAAKIISSPPPFFFOOOSSSAAA 1II0OOMMMINNNPPPFFFOOOSSS PPPOOOAARIINPSSPPFFFOOOSSSAAA 1T0MION PUFPOSNOPOPRA IFXSOIPPFFOOSSSSAA Masstyne Sample Uist P`SiameedleFLiistOCtW3A20S5S.5L8Y.40X1038 risPROISamia0 022994591 Paez PagePoston: 2.1) InetFlle Botte InjectVolume SampleType ConcA ConcB ConcC ConcD ConcE ConcF 531 iitddouumnnniiioooesssss oo1tr i oneioes 2 0 == z =m z= m == . z== == z2- B= TS 2. = = = =z 738 oolnomemninciioasss &3 0 z == z=S-=T=T zt=t= === ===z B33o iiidoomnmenniiioccssesss 78& BZ== Z =SES z==T=T=Z=z ===z=z= . 31Wo iiiddmmueeeiiioooeossss 1of1o i iomeioes 12 w== =S = s2= Z z = s z= === === Ss z= = = = hee 3Ii illoammnenaiiocoasoss o114 8 SSSEsTS =S$ =z z= z= === === 2Bi iliooomnmnneiiiccosessss 111788 5 ionnicas 1 i=Z S ZST Z Tzz== =z=z === ===z 1 gS =~ === i22 iiiooonmneeiiioocsessss 211 i ioneioos m 1 0= BZ STz I 2== =zz== ==z= =z2=z SEE = B2in liiooommmeeeiiioooseesss 4251 5 loneioos 1B=ZZz Z S ST T S =2= ===z == :==Z w= ss ==: = 5 335 iliooommmneeiiioooesosss 321 3 iomnioes 4 B1=Z= S==S =2= L==f = == === === 1 TT I TT z = = 333 illooonnnnnniiioocsessss o7 3 lonnioss 8 0 ==ZTZ=T === === === =z== = ZT = = = =z - 33 i1iooomnneaniiiccoaesss o1to ionaices 12 0 1 Z == z== =2 zZ== ===z ==5z = TT z= =z < FACT: DATA REVIEW SUMMARY FORM o-- e 2 2 ap :. ncrswrr ZoLdT vant [GS4 oylLedMitnn] sot Lass, I pam SW Q0 2055 EE 55 DataReviewSigo-Off Neoi sloes [kin QfuioltnytAnssan Ren ovata IAC. 10]28/21 r------ 102149 aa Sil| ee " eS 793 tt Fn (Qrvlotdcondturegr my ZZ: 2710 nt Lakilesgpalfprbths [So din [TREE Coit, oe li Grunt at rin?Bigoena,2 ome 0K [oreo ul " | stable a "o% pocaonstine PEA 7 =F77 70opi | p do mtfi i FErcis es ; loa 77gofp ls _z.02%0 Eencetsuak </2L08 sev 6. LOO. | (R oi Ll nbriotl ludinl. % JetsonChaka: _( 1 RN perce fade nobwiedsi dl. #. {0277 . lose(5) wire ost ACLol ]5) | hose: Sg 007 |nccpabie LinerRange: _ J i poo= eometma_ A Checks: hesnso. lows sane. -- S-- D --_-- S-- E --rs=s 000620 7STL Tims, me serio -- . TE 2s -- Seman EEE HEE MEE } ls 000621 bho wend sald Be tn, wll sh repcked Son olnfae nds Thc deka nob wed. Ue joule 000622 iE EEE Wa Bok torgh andy -dak nob wed be laf 000623 _-- pi [[(CRCCoeoosrmvpptcooanousnnnetdyoc1founD:aommEmeT ix:naPaFrtOos SSri;c0A9r93e7a80563347 = (Cre yr ina. Gra: Exe Weighting; 15, Ais rans: None 43004 . B C0S24 [(CCCoomlpootuneodcurarSvmeo;era:aisaF8tFi8Oa5SnA:o+9n62571961.404 [sipraereoseyLo:iCnrOtarmg,SEcxAorn, Waigheg: 1s, A ans:None asm . a / TM00 2000 400.0 600.0 800.0 Soe 0r0625 a . | Coreen rae et wen | [Response Factor: 3154.38 | (Curvetype: RF i . '% x| SEG ei wee ee isGe ~ro626 "x -_ ey 000627 000628 Tin, mm------ 000629 : 70 B55 Quatro 4145 L*og 9S9O09U2P020199:PFAAGCETE1q1uipment DATE 102890 SATNUDAYLNYUMSBTE.R 0X15. MATRIX:aDsEsSCRIPTIiOuNi: FACTMethod: ETS841 iniccion ol LQul. Celime 0mi, Bode ZS Mobi Phase: omumCHOn GMOH TNA mdm (4) RSD GINA Propet (ast: (WAISmmA GLEE Epis oun mae Sorcuronen? @ or N Over. Guardcolumn: Nee Part sherza Lot umber Analytica colon:KeoneBs Swe2a0nn TyeQlh SpeeseSim PutmmberQISNI2Lotsumber ELL Sermunber QIRQEZ . Asien parameters: . Doing gs(UN250__ ES1sbugals(iLiOLn1g_ SeasaL1h0 ; LCFlow i:0, Backpre6s5h: HPLC solvent gradi10min1095 ahmaw o bim e ssw de wo mo ase wo 000630 oAm or BEST COPY AVAILABLE , poanrcgs -- ES i A & i! (A a0A FF Ley nr cir we am a EEer E y EEimT. i8 =m i E d t ,on 000631 . ---- 2 HPS 000632 [-- E satiR s ris,e, _re--s_o--n: Pmpaseerrsotpyra,tprootary omia-10-93 ontay,eoeteeber 38, 1099 16030148 ree 1FTF aa5 orei8 p Sntoowp Fmimey )nine o108.3300 GSrveenn TTeemmppearraatcuurresLmaatnee(0c0o)d) 358.:00 The Gradient Timetable contains $ entries which are om TTmmhe 3sa80 35 i] 1m0nl8 55:0 o8l,:o0 80 xo3 82n1o53w%08 Fra8pstmure so 8:38 we E5c 50 280 800 830 88::3386 &io The Tiastable contains 3 encries which are : iTtaieeid| a0 ColumSBeEtsuicch Cont&SaEEctl 8 Ste Cont&SaEcEt? Sie ConSSteEatEct) Sit ConoStaaEctd Sie DBDirlaaanwcpstpoeotcitsoen"iatim)n) Stop Tae" (oins) 8193..000 5:68 fffiteion Veitme 150 Massy.SapteList. P`SiarmiieaLMiostn0C1AM2A5SS1L6Y.NS3w12o9u59p70als prSampie0BHI25000.5PL Bases ro PagePost:(1,1) FloName FlaText usFie 21 11lo00a32s58e00s00o00u4t30MTMaeOoOIHBBellaasnnckBSI010B91-2,1S2E0E 1II0MOOINPMMEOIIS PPPNNOOORRPPMKNPPPFFFEFOOOOOSSSAAAISSLISSS 81 111000223839900000444215 TTTOONO!K4XESRRIEBSSI0R810T01SSsaSoarp0uIhmm1BB201,iiSc1E10E20SS%8EEEE 7 igasois TOKIASRE1S0I0 O1N2SNESE 1I100MMIOINN PPMFFOOISS PPPNOOORRRPKKNPPFPFFFOOOOSSSAAASILLSSS IOUIPNOPMPFFOOSASIS 13i0 1iiGg0Ea3ss0eaao0di0ssTTOTOwXOsInAXEBASIBISOdIIOSvNeRS1ooB0pp0oe0Sn0ria1zI,.2OSSSEEENEEE IT10IMOINNPPUEFOOSSNPPPPPOOPOFFAAMOOFPSSFOOAASIIASISSS wy e 1I1i32 i1o0Gaaasss6eaeanisss0TOTTXOO1XdX51RIdDsdSIRSOSNnISDOElS70e8I00SO00201181120302..0SSS00EEE.0EEE I1I0OMMOIINNPMPFFOOISS PPPNOOORRPXKKPPFPFFOFOOSSOAAISIASS 111865 111000B22s55609R9000088824)MTTeOOOHXBKaInIAc4SSABA1S0T0I0DSaO0v1-iI,2RSSoES0kESDaS0-dexe10M/19.0 T1{O0MMOIINUPPFFIOOSSNPPPOOO-RRAPKKPPPFFFFOOOSOSSAAAISLLSSS 71188 2 111000222555999999000856567 102599080 TTTOOXKOIISSKARTTISSSAGRR0OSOSZz4R4eaMMT..DDuSlyyll,.,GSSSRmoookkO,,,DD%OSS3O040eeexx7x111000MNNNAGa.bRRD0e TOKISRTSGROGEAGM,Devt Smoke,DS0ex1OARS I1IIMOIUPFONNSPP--POOOMMAPP..PPPFFFEFOOOOOSSSAAAISSLISSS IOMIPNOAPPFFOOSAISS 323110211080320500000000586100 MTTOOaXXkIIAABGGRaABBnSSIIOO1I0o0s25300u0o00b1C2,OSVEAE.SEE 28 102500062 TOX\ASRTSD.aOy.RSm0oh8o.D2SSO OI0AM, 11I0O0MMMIIINNNPPPFFFOOOSSS PPPOOORRAKNPPPEFEOOOSSSAAASLSS 10MIN PFOSPOARPFOSAIS 222507 2 111000222555000090000808843 102599068 TTTOOOXXXIIIAAASSSRRATTTSSS TOXIASRTS RS3AAOGOSSSa2IsSaaN-M1.DhDaDoavvv.,eSS,mmSooOkkeeK.,,DDs0o500sw.a11i0d1119098805 ROIS 2M, DeptSmoke,DSO.101059 1ITOOOUMMIIIN.PPPFFFOOOSSS..PPFOOOAAARAAPPPFEEOOOSSSAAA'ISISS 10MIN PFOSPOARPFOSAIS 233100 111000222888333000000666878 TTTOOOXXXII\444RAS5S RBBBSSSI22sI55aI00pD00pD0pyO0C1CI2COI,VNV.SS-1E1I0S.E13S,SS00EEEE 3102899070 TOXISRBS10100-2309Pb-COV-11S-E4E IT10OMMIOIN PPMEEOOISSPPPNOOOAPRXNPPFPFEFOOOOSSASAISA1SS8 10MIN PEOS PPOAFOSAS 333162i1800s260s900n707:13 TaTOXOHI4XASSaInBSk RSE1SSaIr0u0BmKN1BIcH2i0R3SOE5SEEE 3 102500074 TOXIASRSTarSumIBa0c1I30SE0E TTTOOMMOII PPMFFOOISS PPPNPOOOFAPAAXOPPFSFFOOAOSSIAASIISSS IOMIPNOPXPFFOOSASS 3337 11100022a58s99000000r77785 TTTOOOKXXIIISR4ASSBRRBB1SSII02ts500op0o1pI1mom1S00511S03,2S.5ESSEEE.EE icaseora TOKIS ABI0somIo 1O,SSEE IIIOOOMMMIIIPPPNNNOOORRRPPPKKKPPEFFPFFFOOOOOSSOAAASSSLLSS TOMI PFOSPORK PFOSALS } &@ 1110002290908007100 TTTOOOXKKIII4A4SSSRRREBE2tsSSS0o0o0oI1Iooooeo000n111II152..SSGGGSEE0S8EEEE 4 loess TONSRESITe00ooIn12G,SSEE ITIOMOOI PMMFOIISPPPNNOOOARPPAKPFFPPFFFOOOOSSOAAASSLLSS TOMI PFOSPOA PFOSALS TG TOS RESI016e 10mm 12 SEE TOMI PFOSPORKPFOSALS. omy e --r ------ ce ----------------G rs Masatynx- SampleLis Paez `Pi rSoatmeicaMiostnRCOcAiMR2A5SS1L6:Y3N2K:57so1u0p90ats prolSr ampleOBNIC25090.5PL e mimmmP--ag--ePostion:2an1 InstFle Bottle InjectVolume SampleType ConcA ConcB Cone 321 iitooommnnmiieccaasss 2oo1ir 0 T-- ==- === === i ljlooommnnnniiicccessss 52 Ea zzA z 10 Saws asm im 3S7$ liiooommmeeniiioooeessss 3237 111000 SSSuwundndwdesad 24G0373 aa6s3m === - B11 iiiooonnmaeaiiioccsaasss 232 : 3 omnis 3 111000 SSSuuanedaaddd 10 Sued 2Gdms 0a287 === 77= : i1i8t liiooommnnaniiicooesssss 3o i io =Sunes = ow w om z= 7ii7n iiiooonmmnnniiioocssssss 3 3 ia2= S=zE z=z=- .=z== n32 iiiooommmemniiiocosesss 32 1110 =o6c8 HZ2oe dd=ee === 3#3 iiiooonnnaeaiiioccseesss 3@3 fiBr o = So s zZzJ= iI2 iiiooommnaaaiiiccceeesss &a p0e z A Tz z o=z 883 iiiooonnnnaiiicccseosss a2& iiio g888 uH2se ddaeae 253 iiiooomnnnaaiiicocasssss 2 0j&=x8 d=T .e z 3337 iliooommmemmiiiocossssss 2224 i10 =Swdws nXEsD,=im === 33 oiioommmemniiiccsasss 2335 1100 SSSwwwaaewwdss Goa3rs 36sma === . 28& iioobmmnaaniiicceesss 223 11000 SSSwuwdnewedd 24or3a baG1a7 === 4& inonaaicess 32 1100 SSuunnaadd Tasw a7m == FACT: DATA REVIEW SUMMARY FORM iT e tite le | GaETas | ose P ll eDataReviewSign-Off. ous wie oa pra ON) pres crs Zo LUST vans Lot 00istry log IL Nan ouisofas oe| lh WIEHS Sati 122954 QSualaAarRve Sa 2299 odo orate __EUcn10k)26e /93 d mee POY 570299 Comms trons "oem Ot4tetitrtenldoinslper be _a 2252, on Gi aBe 12 JOO | ay hA awt2b 28Loe weDliee lastgwlr ere SY ded cult Cet 5% base __24D eeert Rg _ hr _ en -- mar _---- LL lo Hy rin of Re ese fli Z 2 hanes ---- CL ase3) 2) _ = --- Prt &or I ot a Oss,(ovcgiremodcmbevrinl Quen (Lesbuac?), None poms 1) Conese Game Aut Sylicy Nose ElapsanSGruUECENT garmaac:HETYA5/T hts [R-2: cra oe MavixBlank: _ : [MsmsD: -- [DuaSusu: bois 000636 . SE -- oo pi =~ HhEEE E CHa EE eEmEmeRwn OE oBg MgEht hd Holl - 000637 pEier pegoi 0 HiEi E EEEE SEA we B152 OoREuE "i, i HEE SCEEN h E fhm omRmmERESENEMDR Wom ue oF am o mp |W cu anent ef+ aps resoduch roseLoch woh8l9 000638 Bo bgt ale del nek wd oo Ve fgg 000639 . CCom mo poa o|frspaemes:n rFbmOpRaAs? oen(r59cn9)92e6s |Retys pe:p Exteo rnaa lStds,Are ea 3150 |Curvetype:Linear,Origin:Exclude, Weighting: 1/x,Axistrans:None TS oo2r 735 . | 5 | 1 . 00 2000 4000 600.0 800.0 fo 000640 p SE e en |Com2p namo e:9u 80(n fund c2) ho |Res typep : Exo temn alSts d,Ae rea |Curvetype:Linear,Origin: Exclude,Weighting: 1/x, Axis trans:None aaA a hasan Tsar 000641 RCCoemopprmceo 1s5r05 [Retys pe:Ep xteo rnalnStds,Are ea. 2325 i a : "Oo eos wo discoe 000642 oA 000643 - ~ ) 000644 000645 B75 QDuaAtVrEoYUn0i7m0:79#93:6F1A08CT Equipment Log 99077 PAGE ___ DATEL019 ANALYST:nmb STUDY NUMTBuxERMar:i: RatSera Lis DESCRIPPTOIAAORN58,0 FACTMedd155 12.87 IjcionvoSlAuLme Cycetine: 10m. HlecrodeZe Mobile Phase: Gnomomcnc eMon ld TorN soldA mber (A) 90S Omazs Prepared(date/by): Wlpysosh Epes: wow Seruromem? @o N Over mem (B)10239sa9b Guard coum: Nene: Pmmbersa Analyticalcou: KeystoneBeas Sslxbmm PaousberOSSINZ Anaya parameters: lommen S-- TeeCl Seesaetm LotmumberOELL SealoumberGIRS `Dryinggas(Lie):487 ESInebuglasi(Lzh)i1n0 g Shge as(a LAt )O h = LCFlowrate:u30l0/min, Backpress8u2rbaer: HPLCsven grades: 0mia-10:35 Tnmeemam| ) a jo ni mam me me am 210 me 0 000646 J0P25T ning Method Report mage 1 sathod: C\IRSSLIN\OAVEY. BACKUP AUG9SRAT\S,FDROAAGQVUDEBNBYF-S Printed: Wad oot 27 14:31:21 1998 ISBEL ERReEeea =R peeoe h CEE Z 570Coy7ahMn INRIA ERAN some (ese) fCSeayrpaiptoatzey1tn COrNRoaapEatls 2 te. Scivation Tem f-- Sanartysner Vacuum set nak sFaOas 200 3ooop0 ae 1% 16 pr oree a Anaiyer oTonneerecssay31 1 SSaii ntei, oTitoenerescsay222 iciptser Gar iow Sceorneetvcaatsion see nak wh3as BFiE oas 15Li%0. es nak 0We 27E27 000648 Method Report MLeatsthodModFiifliee:d: Printed: Page 1 WC:ed\nMeAsSdSaLyY,NXO\cDtAVoEbYe0r702779,3.19P9R9OVA1C4Q:U2D5B:\0510min. Wednesday, October 27, 1999 14:25:31 HP1100 LC Pump Initial Conditions Solavvents 90.0 Bcts 1000.00 + ol SFtloopw T(immle/mi(nm)ins) Cow wr MMaixn PPrreessssuurree ((bbaarr)) OOvveenn TTeemmppeerraattuurreeRLeifgtht((c)C) 1000.0300 4000 33000.00 HP1100 LC Pump Gradient Timetable The gradient Timetable contains entries which are : Ti0m.e00 9a0.0 1B0Y.0 c0t.0 51.l5000 s05l.00 9510.000 oolloo 87..0500 90s2lo0 10950.00 ookloo 0ov.0 0F.l3o0w0 oolloo 00..330000 oolloo 00..330000 HP1100 LC Pump External Event Timetable The Timetable contains 3 entries which are : % ITniinteial 0.00 ColumoffSwitch Conotfafctl off on Coontfafct? on Conotfafctd off Conotfafctd on 0110 off oft oft off oft HP1100 Autosampler Initial Conditions DErjaecwtsSppeeeedd (ul/min) SDtroapw TPiomseiti(omnins()mm) vIinajlecNtuimobneVrolume (ul) 220000.0 09..0000 112000 000649 Mss -Sampi on B Sama LtR CUASELYE AXDay,0CT00PROSOmACENGZTS0 9% Flatame. Fie Tex i 1I1 EpmE eRnn R pY MoeS noew E ecoaE u tmu y i i EllI anRm S ou Iuu S r izi R eis , TazAr BI bH lEE eEso IE e onr emRme EE ey, i Bi J EER s a IoCtE SeE n--, o------ BB jBEIaEE EeRRTIoIInNSeEMSmTNiSR HRmiimNm tbDfuuurocomrrnineeswoonnroaenonneeteseses BBF BBiEEEmaRERnh II o SNHEET s nseoS evioen m tooondty,orcoeT nesateeanitootnnee: B53 BEOls aSEne ISeISSsSoHReNsRmuomaTotfErrwyomoree eeniootnagd B33BIEEEEEERlRE N ISSSiENRI eTEmeRn, ofdtonrnu. sssmmnmooetreeeppDDeeerennsoeinnsane:e 33B3 OIEEEEERReESE ER SMeSsHEeEnRcIn,oSoontnstmyrootnespeoxeinasnen: IER tu trate nen 3B4IEEEEREEERRRS I S e SH E SEN R I R SOCS tsuR T nrotgI oetI ssaeE ioxeS E ene, i iPGRR ReEdIR eSNaERmLEmy a or --t--e w--i--e 8o LiS l mE S Y oIuSsu toE eitcn eoenoE wum,e rmtua, 8B 8 EllE EmnRs SIN SSiutiee mes nnn nieemm)raea)na 8 ElElEamsRm iS I E een nmme a aans mnn, rd7Ty aot [---- 000650 Means SateLt Staenleit CUnbIi ASTOSTS LROYSANTONETOO aSP. HOES Paez FagnPo1) use ItFla Gotta ijtVolume SampleType GonAe 1Ii1 rIIioMmMuMmMnEerESSoMRmuEsSiRiEsSs merReRiE nesi 3o8vt PBBe zz -== Bz - IOIMMRMMMMIMEEIERRe IeSmeREil 33 BFBE$ asSmwaaas =--Z on BAB8:b Oi IRNRMEaOMEWEEnRRRE emluoieereislse | BBBBEaSEeEaaEn = B IIiOI M MsIoRnS I ReEtS R iEneniE teesl 37 BBBBzsEEEE ==== II J OIOAN IMNEv ERME NEERRES imeeeetRRsses i BBBHzzze -==- 5BB ZIM aR NmMiEM nIS: mImGmereenieiss 7 BBBBzzzz =z= B5AB: OOM hIIaISSrEEEefpBEmtieeeatnese 88 3 jijBBi zz === = B$53 OOMR BNIIM MOSNI IEES BBECnreneeseis b222h BBBizzz === = --- 3$p 3 OIi IOGBMMMInIMRNNEEtERRSE H eEenere isss 3 5 BBBBozza == - F33DIMmmOMImiRIeMnNmESEiSEMmsESsR BGEBeeeeiinnsss 2233 BBB=zz= =-= ba 8G88 EII MNNNSEE EESSE GErBoimereeeslls)&& BBBBz=a ====z Go 8 OM MMIR ISEEERS Br BrRreeEel | BBBBzzxa =S== 828 IMBMIOEmRNRwREEE:N emmeheeeieesees 133 fr ers i) B@BS zSeSewaa = =oC Bawa oC 52 INI iNEunIN eZNoEniI IRlEt mHmieeRneeseee | 8 5 NEN limi b BBBBaaEEmmEEaa o=o-CC Rama 000657 . ae Mass. SampleLt Pes DSFeaiEmpeeieidCOeUaAaSeSLorYNRe1sm TheHRePeROSeATHrADEeNCZeTS0s5.eeesmerPagme peorne1:,2) Flatame FloTest BOT ToSRES10 02 s, RW 000652 r Massye same Listre.-------------- 2255 Paces PS-ia_mapWlasetdC 0237U14A54m1S0y0970S708L PROIY SaHGNOROZR1005DSPL. Page poston 2.2) us Fie Inet Bote InjectVokume SampleType Conch B OMNPOMAROIS fomin1oBS 11 0 sud -- 000653 brn crs vations in Snir tc reo Tl Ts Tas Plc: _Joptte is thay iy cm FACT: deends DATA REVIEW SUMMARY FORM DataReviewSigno Nounas Yhely ed Blu ed Nome Mv-5-79 Qouilnl AorannBaie Dantary UC osfas [---- H0alideset ge 1-2 "ists . foe rte Ha | ohs tdr cuolh atifen N.S `Extraction Blank:_A/4_ per_ meTto RA Te poa re Own"7 ueules = be Zz Ud ye ipdascided om fe wipes Pos con) hcoonelitoge o__270,4 Ip"fil Ecru fo a 02 or OTE vesas Luss des fo0 [cbumemnsoenchotsou08 eBorgefslaomc7 hin--l7a4p, bosune doied cole zo 10 di f ov; -- Ae bel pa ld fybadff2 2gas the, ? 5an 4; He de EA euros Cun) rr [AchcoeoptableL_inte1ns5R,a0L n4ge: d #93 S=e2p c upkfhos ls 12) obbsuved r. or LB eo fon dha dag | --A ---- MS rinses &_ fiage fom ccmitl | ------------------ rp. ok 2 | ------ orate Erlend --_-- Tatlin plo 000654 FACT: DATA REVIEW SUMMARY FORM p22 - o, } DataReviewSign-OfT Wh owneine _Tas oncrsys Toes Naming:Ldn p20dosed i _L888 unos Tas hfs J-- re ; Normals cdhy et oes soon QpuaiyAomara Been Detar Ue u/osaa Commens utctns Ue ulbslin ET) EN La BertonBn: 14 SS Mae Bik Coie th hens . ! fone I! a -- oo [---- [re Masix BL $) bere, [CaibensionCheck: 15 v> foes : on | A oa [---- Je a Ba taser hsp oles To set pe 000655 000656 Re Brrr ok OEE 5 I GEE i BIESSi SSFRs TNES. LHiopHEEE mRiP | oaHS | pi ERERERISEEMES. LE EE gr U0 000657 000658 000659 [RRCemsopemopanosruenFsdp1poonc:aamGer:n8aP.m6Oae8A3AS8u(,CAhir) - . * . x - * L % 1 O 0s 6s oi 65 ds 67 ds os is 000650 [Roceesolmphaootnpasonenot2pocfuonu:Daenma:Eerdx:iianPm2aFa0Olt.S4nS7d:(5.C80hA324)r81a00787.2710 : 2 er ner, Grin: Exc, Weighing: 1, Aas s: No . Rospons 00 200.0 4000 600.0 8000 Se 000661 [C[arRanceCsapotomnesperc3oyoufeunrD:aenem:Eaedr:a3mmiPea4FstOoSS5nAd.x.(67.AC9hr93e607)4a73.400762 : irv2e4ty1p0e: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: None nn] x Rest 00 2000 4000 600.0 800.0 Sere cr0552 C[[[RuCReroosemspsppoootnunsnesedpF&aAecne:aemrEe:21Sx5u3rr5S7(eiCdh,aA)lrea | |e i i3 i os 00 01 02 03 04 05 06 07. 08 09 pd 000663 \ 00664 EE. ET 3B ~00665S ww ENE = . 000666 EH ERme------ 000667 : os Usit Quo, settee SOUP020199: FACTEquipment Log 99092 PAGE U1 DATE,11-0499 rUBoW.OMBER.TOXISS MATRIX. tse DESCRaIs2P:dToeI& OonNl FACT MethodEIS 51 ctonvolume 10a Cyline 0mi,Bode ZSpms `Mobile Phase: (A120mMNHACHICO2 ToAccobmobe (BOSSI pty: WANBa Exes wows Storestroomeap? @ or N Owen MOH BINA GLOW maw ------ Po oma Aolconms Koreas sue2s 50mm Te.cat Fanoumber 552012 Lorber ELL J Sealounber OS1A04Z Anta arson: Drgay s(Li h):n 350g ESInebuglasi(Uzbe)i1n3 g Shgaes(aLt Q h LCPlow te Miulnin. Bickpres: 5h HPLCsevensgradient: 110 095 fm br awrw-- e o vimm me me sam 00 00668 BEST COPY AVAILABLE untag Method Report re 1 p-- CLASSOUR02RO0\MEG1UDR9APF9OS3 sence ou dow 04 14102127 1999 (EE 4 EE= | = Re a| z So somce (esr) Echarpeaicttaorry SSoeusroiciaevnatBiloonck TTeemmpp sec na $W 1603LLe 3 0520 1s f-- na Aealyser Vacuum ses Gas carl 4003 ix wr iwTotonnRnEeessnooettruuocciioonn WLioeinntsEetneerdry namo ar EaxotlfRRaeetssooblunuctiioonn LiSeoonnne'Esnheocroeyy namo fetnit5 er see nak hi10o0 boseoos se ax F1o0.08 W oih s $so d 000669 gh oe ------ El si 000670 [p-- Rll, setataas 1100 10 rem Takei Consiticns sotyancs a5 PENeli5opwrfr(ieens/eemuit(nem)in(eh)ar) NvOvaeeynn'FTTreeermsppeaeurrcaaectiu(rrbsearL)Raitgeht(s(:c6)) . feta rta estar, Movember at, 1999 13130130 oo i80 28:0o3.300 w$8s0.0 . The gradient Timetable contains 5 entries which are "i tiBomheeo. s8atnl 1i%e ol8:o8 oah8 n8o35w688 53i53e00 ws5al0no 33o:0o0 ssseoe aa00 88833:663006 mes Prew0se%suce &ii "he Timetable contains 3 encries which axe: mTiiptmeei|n ColSumiBettSviteh ContS&aifcts CoaS&tEaEctz pt) Sit Se Sie 1100 Ascormmpier Tateia Comtitions DEBjrieaacwaepsopBaeoiencdeison1/(ommi) n 28003..000 fSIreaeefipteTriiao"s V(oalintsymeq) 155:50 CoSniStEatEct) Ed ConS&tiatctd Ste Massiyns-SampleList | `PSravmaiaTLihstaNCoAvMOA4S1SL3YN1o8u1p29092200199 pr SanpieDB 104008.5PL Paget PagePoston: (1,1) Fiotama FlaText wsFie 512 11T1ii00i0is9e0900000001nnMMMeoaOooHkSBBaaannnkkk 111000MMMIININNPPPFFFOOOPSSOPPRPOOAEARPNOEPSOEOSASAAIISSSS. 18 10111i0001i3o00o000005s TTTOOOsXXRBIIS1Ai1SS0SSeARur0raTB8BB-ii0OsdS2,.;eII0eoxxx.IITH1/BBGGSRORNSOMNMCCS0CHHH 111000MMMIIINNNPPPFFFOOOSSSPPPOOOARRAPPPFEEOOOSSSAAASSI..S 583 1111ii0001ii3os0oo0000007nn TTTOOOXXIINi4SSSAABBBSSSII1II00O3S5NSSsoopooommn,,,oxewixx.tI11A11//R33I80H00MCEEMHH 111000MMMIIINNN PPPFFFOOOSSSPPPOOOAARAPPPEEEOOOSSSAAAITTSSS.. 11I20 1111ii0001ii8o00010110s TTTOKOOIAKSXABIISI4AIGSaSS2RStA5oo0pEBo0mw0oS0S,,oHaexIxx.tHI1IA11/CB/G43O9a0M8SNECDCDHHH 111000MMMIIINNNPPPFFFOOOSSPPPOOORRAAKPPPFEEOOOSSSAAASILSSS. 1i1388 T1111i00ii1g8009000111345 TTTOOOKXIXI4SSIAAdBBSSS1IRIIBGGSSDHTsosI00Gmep0aDb0S,.-euann1tx01110/173309B08pO00MNRCCDCIHH, 11TO00MMMIINPPPFFFOOOSSSPPPOOOARRPKKEPPOEFSOOASSAIA'SSS.S. 111878 1111110001:1090200000111878 MTTeOOOHSXaRnkIIAS2S255R00Ao00TTMMSESDDS.IOe!I.x.e1x0I.V1BGVSOAMBa0COHMDCH 1T100OMMMIIINNPPPFFFOOOSSSPPPOOORRRAKNPPPFEFOOOSSSAAA'IISSS. 2119 111111000101500000000221100 TTT OOXXIIAAO SSRRTTSSSX RRGAUISaII eNGa0S OOOwm222555A .,oo0xx01T I1VIVSAABRVOS OMMMOSOHHN 11O00NMMIIINNNPPPFFFOOOSSSPPPOOORRRRNKPPPFFFOOOSSSAAAIIISSS.. 5BBRi 1111000101000000055534 TT aoOdO aXnkIRX AIFS5MS 00ROuCBO)R N2SS0,,ooItxT 11IVIIABROS SONM0CCHH JOGMMMIIINNNPPPFFFOOOSSSPPPOOORRRKKPPPEFFOOOSSSAAALLISSS.. 2275111101100013050050002887 TTTOOOXKKIIIAAASSSAAATTTSSSsISRRROCOESNzaSsiMoS SomOonbyo 2G6O,NoDnh .1eoxVx.Sn 1B1/IM3AC9RH0. OMMCCHH IIOOOMMMIIINNPPPFFFOOOSSSPPPOOORRRNNKPPPFEFOOOSSSAAALLSSS 23110111115004103300000520008TTMOOXeXIoIAASdSRaATBnSSISIROG0S26I0A0S00CmOVIO0,Na G1xAD.B110M4N0CY0H G,H ITOOMMMIIINNPPPFFFOOOSSSPPPOOORRRKNKPPPFFFOOOSSSAAA.LLSSS 3331 111111000410353000000805182 TTTOOOXXXIIIAS4SEERRRTAOSTSSIsIInn0Oo3is.z2as4so0uU0S00mComOrVoGoO,nNextso,1eexxADttB1NO11N/142YC90H30YMC,HH I10MIOINOMPFVOIS.IFPNPOOONRRRPAKPNPPEPFFFFOOOOSOSSAASAISSSS 333850 1I11000iiis0so0oo00nans:e 7MTa0oXhO4S6XaAnD1eS4I1ES0oA0aB0BIBoHO.,2Sae0xxI.H10A/0O%0MMC0CHH 1{10C0MHMIIINNNPPPFFFOOOSSSPPPOOOAAAAPPPFFFOOOSSASAI1ASSS 333785 1c100iiissoioooountnsro TTTOXOOI4EXXAD1SII41A0%EESE1po0ARu0nb0BB0,Tsa.S,xxSatIx1|I11A0AABIBROO0NMIOECM0HHE0H 111000MMMIINN.PPPFEFOOOSSPPPOOOARAAPPPFFFOOOSSSAAAI1SSS . 4 TT111o00ik0o0e640n1e TTTOOOKXXIIIddA5sERRBBBS1SIII011a0D300S10o30o0p006,,e2oHxnexA811|B0AVOBA0ABMMN4HCHH 111000MMMIIINNN PPPFFFOOOSSSPPPOOOARRAPPPEFEOOOSSSAAASSS. GG T1111000i130509000041u55 TTTOOOKXXI!4I4SS5RAABBBSSS1II11I00030%0s2To5S00cm0om0oo0b,,,aaaxxx 1111193B00O09MNNCCCIHH I11O00MMMIINNINPPPFFFOOOSSS PPPOOORRRARPPPEFEOOOSSSAAA'TSSS G TI33048TOKIASRBSHIOS-1aox01o17p08m8MoO,H TOMI PFOSPORKPFOSATS. Ss rer sm C enor arOi s!|{amBmEmeaenEEalan i!i|PPEEEEBERREEEEEREEEE:] ihaE1BiB EEElEoEE mEniERogEss 10 PPPi BEEEEEEEEE RREE:: PEEi [EEEEaEmaaEmstt:e iIii I EBBEEEEmaa8Rm!ttEe Lowo.o ef3i3iBBEEEEEEEEmmRmEEEaeGs:dl : i i EEEEEEaEEt VewgfgezzzST oZOzzmezzZsGorot G=oozzre ggggfgzzzzzz:ZzzzzzZzzz ::o:=Zz }B fBgg:zzzz ZfCCz=Zozf=I:Z= :s:Z=== gB8$BEz:2z ER0Zz3IEz:z%%: ::Z:2 gg gg BBzz:- 00f f ItIfzIoo::::== gggg BBzzzzz fDD:Iff: zEZo::E: gggE iz0zzz EZZCzEZZzZ=:==== ggg 8gzz2z:f f C2 Z:z=o:2== nro-- - Coane acts vorsnons Se seOapwrens FACT: Wo Tis Tou Se dottashi tpt Muy Ghehr DATA REVIEW SUMMARY FORM DataReview Siga-O CNet zed Bl Nitin ufo[kp evson QNuaeyAonsa Bere owser_GALWEI are Potcan Loo Runge 24 hOF emSA. MExaraectBionkBlnk Aq SonChio m __ptt dclentis tunesseh ow tree PBal roudo--p Tas tlee : | e_Pogen ET loa __2RL ex _asmers Enc Bankes os og vax uk:ba dlnLo ubdonChak: OF = (474) hesacso, li lowssume ohed Tha 4 Golo Duo elt? re _ZosA can) wet 23>d 70, oo ftpL [Rr lex _o.t91708 varie uo:Jtdla Lo ibevionCheck _OF --> sao oun sue Tot (10d ~6 o hall iriLoerege gpspnl fddd -- is Yep Zoho] oH aren conde on soon tte Loo de 4 de lilo Aiche Jas Arh. n EX 7 He 000674 FACT: DATA REVIEW SUMMARY FORM - Jil } DaaReview Siga- wre J oie tly acts pg min DlSa=dy 1di cule od oly Voie lic bo sapons Sete Losin Qfuioltrymmearae Rien nnn _ yay cle mens Sip urs : [OT PE NP Comma etcnions: are Ser (y)OT"! obi 4 ic ub 11 fe rCo ot MaeBc __an ous sone --- a: - LinearRage: fe "librationChecks: besa: Jso e eet 2iy Mae 1 i hose: epiiar loa [---- le ase oosvase ____ usp lose Ta Youfrr ' 22 000675 _ tpl 060676 000677 a = RE OE EI | OE ha EH SEESESES B | |rio Enz Hi x oo 000678 000672 . [[[(CsoCRmppoooounmnvFsd1aBepepcneFa:mEoea:7r.nPm5O5asA9A9Su(,ChAir)ea : a Fas "og5lLsLsY Rospons x % di dr di gs 65 6s 67 6s G5 1s 000650 ficienntdo2f nDaetmeer:miPnaFtOioSn:(C0h.29]99549 : [Rebsrpaotnisoenctyuprev:eE:x2t9e.ma3l77S3*t,x A+r1e9a1.305 type: Linear, Origin: Exclude, Weighting: 11x,Axistrans:None 2. "1 x 00 2000 | 4000 6000 8000 Sere 000681 [(CCCoolrmorpcaontuotnndocfunDmoat:2orPa4iRo1nO:S63155A83]8807000387 [(RCersepsonsoae ypnee:aE.xrgt:iE.xAurecae Wolghtng: 1,Aans None / 00 200.0 400.0 600.0 800.0 gs 000652 CRCoroeerionsndep4eBapnr:raEmeoo:eSmunrrrS(dsC,hdA)esa : 2 so . | epee | . Se 9 dz os 64 05 ds &r ds ds is 000683 ofr putt _-- . PEE. BEST COPY AVAILABLE 000684 eines ep ee ET, BEST COPY AVAILABLE 000685 == Br BEST COPY AVAILABLE 000686 ELE BEST COPY AVAILABLE 000687 NS Quatro,#601 L"ogS9O90U92P 020199:PFAAGCETE1q1u1ipment DATE:L055 ASTNUADYLNYUSMTBE.R.TOXI145.MATtiRaDIESCRXIPTI.ON, day20: FACT Met: ETL Injectionvolume:10ul. Cycletime:10min,ElectrZo:Sdprea:y. Mobi Phase omMCECR MOH TNAorsnd ber (4) 9088.15 omany : Prepared (datefby): (A)100099sah (B)103099 sah Expires: wos Sore atroom emp? [or N Other. 200 Guardcolumn: Nene Prmmbem Lotmabenm Amielcol: Kevan Ba Swan TpeQl Speresiesun Parmanber 0387012 LotmmberPOEL Sealm5emb0er 1007 Anyaparapets: Drgya(iLWnRI5g0 ESTebuigisog (U1 LCPowre:Muli, Back prs bar ShegasaLtAIhD HPLCsivntgradiat: 0min1095 Tnoemw--a oJsog am lo vas 010 sa me sam we 000688 BEST COPY AVAILABLE `Tuning Method Report : watzoa: ervassune soea0199 mmo\ACQUIRPFOS3 rrszea: ret vow 05 13:32,05 1999 [WE B H EErA C Ev EE - lg | I s soncz (ane) ScEooxnptetriatcatroyr DS5eoruerTtcaeenastsitoonc:k TTeermo sz see na $ W 160nLLeo 35oa5 asas ss o1Totn"RReeSsnsoaolruugctyiioonn iLIeonnap'Esneargy nam Pressures Rank aatyser Vacuum see Gas cont fem us2 CoxotrlRRieenssiogolunuctiioonn LTSeoonnna'Ensneevregry rasp MLenta g5 e Pi 7-555 Page 1 se nex oi10se.0 i osso Set Rank Fi1O0N.0 o3o% a ioso u 000689 72% 000690 Masaiyns-Sample Uist P`iSevmpale sFitNoGvA0MsA1S5SL2Y6N1e26000u8p020150rolSamp1a0D80E8N $91 i% Paget Page Posion: (1,1) Flotama FleText usFle iol 251 111111000555093000000525080HMaeeooOHnBbbauannnkkk 1ITO0OMMMIIINNPPPFFFOOOSSSPPPOOORRMNNPPPFFFOOOSSSAAAILLSSS fT1o0ommmin111000.:.696555 81 11l1100o5399o00003s012 TTTOOORXMKISIRAESESSSRoAIaraITEBSihOScS,hHeeIoxxZ!nt.IIO11IVUIBIOAIGKORONMOMSNCNCDC,HHH IIOIOMMIONNFPMEEOOISPSPPONOOAAAPAAPPPEFEFOOOOSSSAAASIILSSS 1looommmnmtinoosis0ss8 337 10 T1IiIoSOSNNOdSe TTTOOOXKIXAIIGAIAUONBSsSSSRoIRhbBIoO.BeSeorNtxxSi1IIIcIUVAASoRBIBOmOONMdCNMCH.HH| TI0S68007 TOXI10%4soo3oesRortE1IASRONICH IOIOIIMNIPONEPMOFSOIPPSOPNOOAARMPNPPPFFFFOOOSOSSAAAISIISSS 11J0oommminn111000.994335 IOMNPOPAFPFOOSSAIS 10min 1095 m. y 1T 123 IO110S83O I00M08 TTTOOXOIX4XSIA10BSI%SRI10d00I00B0000Ss00S,.NeuexxSHB xtt1011IAA0ISRBAoRGOOoOMM!MyaCOC.HHH 18 110530041 TOKIOIS 7A80000S0, exR/EIBOMSON IOIMOIIMNNOPPOFUOSSPPNPOOOAPMAPAPFFPFOEOSOOSASASIAISSS J1l0oommmiinni1io0o9ss3ss IOMNPFOSPOARPEOSAIS Iomitoss 1I18T3 111110005590300000444343 eTTdOOBKaKInkISTST1R0S00ES0S00S00R,0oOxH0.nS2VO0AE,To0N1N0RCH I0 TI0S90045 TOKIASHISSSRmoOgSONsEsseO1M0 IIOIMOOIMNIPMNFDOFISO.SPPN.OPOAOPMARAPPFPEFEOOOOSSSAAASILISSS TT1ooommmian1tt0oo.se6es IOMINPFOS.POARPEOSAIS Tomtoss 31315110T15I91090S0094k67aTOTTOKOXIINAASISAAATTSSSAURRTCOGSISOSEHORMNOSSInmoSoATkG-OOGmANOGAM%mN,OEO00NS11G,D00 2 1105990i9 TOXIUSRBSIICSS250om0.CO,ox /ABOMOH IIIOOOMMMIIINNNPPPFFFOOOSSSPPPOOOMAAPRRPPFFFOOOSSSAAAIIISSS IOMIPNOPMPFFOOSASIS olToommmaatt1oo0ss0ss6 Tomr1o.s 3335181101180103008000555120 TMTaOoOhXBXVanAIkSURSESSRarIaBOIXSIeeHIxxA.I1|OVACBIMIIKIaMMSGCS,HH 2 110590083 TOXISRSaTrSs,Ia I1/GROSNCSH ITOIOMOIINMIPPLFFPOOFSSOPPSOPOROANAPPPFRFOOOSSSAAALIISSS lJ1aoommmiinn11i0oo.st6ss5 IOMINPFOSPOMAPFOSAIS Jomin1tso 722110115130050000005%546 TTTOXIOOASXXRESIIIIUGUaDSSS1200R000R000dE00:E5.eSxootnnSI1V1VIABIABNBINGNGCCGEHHH 3 10590057 TOKIUS ESII0dh so0005;ox 1/IBONCH IIOIOMOMIMINIOPNFFOOOFOSSSPPPOOOMMAAPAPEPFFOOOSSSAAAIIISSS llTooommmiiinnn1iooossstss IOMIPFOSPOMAPEOSAIS lomin 1055 33311101501991090590008605808 TTTOXOOISKXRIIESSAORSs21080ER000o00000S0E00d53...ISe0ox%ttIT{1IVGIIAUABBTSOIIIMNMsCNCCHHH 30110530001 TOKIS76R000E00oSx|I/UIOMGCH IIOOIMMIIONNPPMFFOOISSPPPLOOOANMPAAPPPFEFEOOOOSSSAAAISIISSS JlJooommmiiinnn1ii0oo0sss%t IOMIPFOSPOAPEOSAIS Jominioss 35 110599062 TOKIAS1R000E00S0,oTxI1VGABSOMsCH IOMINPFOSPORAPROSAIS Tomin10st ' FACT: DATA REVIEW SUMMARY FORM Fasrafrr 7 2A ne hel DatsReviewSign.O [I Ts ele ---- sor:Tuy14Bl al 2 ay RoleI soften 10]18]55 Stic esti QT ueAreBi er e Bee fel 7s nn Sup ozo ouatam AC Iolai[4s JR-- faa tan @ cpio LiesRasps:MoGang Boalp =see fonands he oo mn e cecenea un on Coe Oltsad prodiPn te Gl dcooemescoi decoarlelAn ar 2chhekwldcd adBeoerSne . Sep ll ben fi sulle sre. ovasune Ske uu; ola]34 Er a epimT wots avesitiProes ndur8"ed7y 2p ) loesearrserSgaepddoii dlnel dcutesidTeoto joa 3-7, ExnciedBunk:fess flaxLog ez a Matic Bln fee ian Log. [outro chets: k= Zug fn pr AesdL sao ok x pb paid Saasdraped 20 fad Ve hoo aod akon blunk ae Han Sime > og Mem fin| Na & oo foge Contos bo Ro 0.909.59F waesunkLsdanod sincvess 08= Boge Lome <3, == C1/)| sao ox es Ene DC hile pt 2i ossrtuse Fons netmp =k TeSplut), Tipp, . y ue,Joesimple seed O 2 PoAe A bedy ir Tro sci reen glo mectarverAyuonur nisults. hh (0/819 / 000692 FACT: DATAREVIEW SUMMARY FORM ~~ +> "hss ot . ; [*---- nctsn vero: Tse D144), Zilli, tel f>d i. Stapp Du/Anaiysc. tm: page Lofts Zs wp ort baste _z5 Can) 5 Lo Range lA eddHoly DataReview Sign-OM NAavne oRveevea lucy VTemarmomRe en Ll bo page QsuttyiAmsses Review 162 re olay Dana Rarry:. WC 0/2134 Comm sons: Alodins. 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JpHiEn TBEDiBESLMELR Te hts Nel ow we pi Ha is BE OSE HE EBE EgEE RES ORE N aa ata Erkend oll thoi os ThhelUe Hfuim SSE DEOSRBESIREEEAR bo tHiEOHEEE TdEERY Caarin 1 PE SSRRN EhThTauSSOuRmbTaeEeE,Y oe Ia if wR i BE CRE o bieo fPah DEED EE EL mmmIIioEmaNE [HIE I HD NER Sms UTe JHHEE B SE Re EEe EEm om omni OFEEE E HES e AR B HE E E Eie fem eeeE ee -=omwee idm mHEE OHEAE R wlein F CoitPine bemisdabokedfsurtihe7d bn ioi3ls9 tao dlbon wilbe sft uc volinlgy 000695 EiEE SEiiEmmEomRnm. hE EiEEEE E Bwmodil es mBEe E oHdg nhy+ythot - je BE i EH I HiME BEER ER EMME EERE Biter 00 te EEE ni CEE EDD iE ne Oly 1a = ELE OE Gull ass comple besorbedfoi ke jae 00069 oto bahytcraoed + 1 wid i ong3 clulcbon. UC 1o2g/g 000697 ee ------------ [Compound 1 name: PORA (GF) [[(CCRoaeelsfbfpirocanitseonent yocpfuerD:veetE:exr0tm.eimn0aa2tl6iio4nd6: 98A9r3e3+a41.55.667792031 (Curve type: Linear, Origin: Exciude, Weighting: 1x, Ads trans: None a Br gh pl 1 ||i || | | 1) 2000 Pe S000 5000 1036C0onc 000698 poundto2f nDaetmeer:miPnFatOiSon(: G02.9)83941 [Relsipbroantsieontcyuprev:e:Ex2t.e4rn8a5l21Si*,xA+r8e9a.0578 uve type: Linear, Origin: Exclude, Weighting: 1, AXi trans: None 31 0 / T oo T 2000 4000e 6000 e 8000. 1e 0650 12060Conc 000699 oC (Como orapcon tfuorDenaet tmo5snuPa9RatOi.nSoAn0n(0dOs+0o1Fb9t)s387891 CRoeesonpe yEonEevartitn:sicAlroo,n Weighing: 1s,As rans: None | <0mes ! | | | | | | | | Go aha ashe ade seh i685 umes 000700 [(R[[ CeFurosvC vpepooyno nspseeom:F4pPanoprEa:omonxet:1u oI5mS4a(nl2ChS0d ad), Area ||| 167 xx E : . Rospor | oo da ds ds ds or ds ds da 000701 edgepray 000702 beni Sep orurt zac if 000703 mB med = i. =. 000704 =a Sup se18s Zasilish 000705 Tos thoes Quatro, poaleE Log 9`90S5O2UP 020199: FACT PAGE Equipment 11 DATE losm0 ASTNUADLYYNSUTMieBER.TOXIS5. MATRIX:alive DESCRIPdTaIaO8N4 FACT Method:EIS820 reconvolume:10uL Cycle te:10 mi, BecodeZSgms MobilePhase: omMNECICR @MOH TNAoroddumber (0) SMESIOS BINAZA Popwed(ey: NJOAOSIaN (IAS Expres oun me Sovumonumg? or N Ober Guard coloma: None Panumber.za Lot number za Asatytencous:KetoneBea - Swimm Teel Speesmim Pur umber. 55.7012 Lotounber BELL Seri) mber05218082 Analyticalparameters: P Dryinggs(LU).350_ ESoebugals(ziUoLg ShguseLtAGh LCFlowri.200uni, BackresusGhat HPLCsolventgodin 10mia-1095 Tbmeemmm 200 msm 1 23m m lem o wo se me 0 C0706 runtag Neches Report age 1 Matha: CsSLES0UP-RATS PRO\ACPTPERB fe rt oct 13 1142033 1999 IE A] SE PR. TEENIE . BEST COPY AVAILABL ws soomce (sp) ScEoatnpeeiatcitaorry SSeosucTriacvneastBiloonc:k TTeemm. sressures soatyser vacua Gas cat waz se na 3$0.000 3L.d00 0F52E am a 660s tae wa 1TixonRReEesnsoeolrluautytiioonn pLTeonnra'Eisnerngy ramp 2 xEioalRRfeeasstooollnuuttiioonn f1Lo5en0na'mEsnnereary rasp ftri]er see max 1io.s0 os 650 a see na 11FO0N.0 2os a FGi 0 000707 C0708 secon sepors p--ri--ce retaces: nor + 1st somaazs.moevaycocmtoa-s1y0-s33 esau, ocxabes 13, 1999 13003033 Sotvents 03 5 a3 il55oP3v5sTrmiemsienurnienin(eo)ar) Ra Poets (har) a1wo50:.8300 SOvRen TTeermppeerraactiuzreeMLiegthctn(ev)0) *$0.00 2100 10 mp orastans Timetable The gradient Timetable contains entries which are - ri0Tmee sRaoteo wleo 8lao 8ol8o n3o3%w rroi1s5ss5uze 3B535E0 spSiee] [f35d5 8i&5] a8xd8 a88:33300 8iiss 1100 16 mp Betarnad vent TimecaBte he Tisecable contains 3 entries which ase - smTiatmten colSumBttsuitoh con&ottact Cont&Saictz ContSSattct) Con&SgaEctd ok SE Ste Sie St Se 8100 Ascousmpter Saicias condicions rBDreacwa:spTetonneddtwe/in 0.5.000 aE5aT3i5ctaTiimombneerVhontaorms) sf355% Massyns- SapteList . `PrSoavemdplFseikOG18 : 115M 812s9o59uA0prisSrOSASmpleL OBNIY OIS098N.SPL Page Page Posto:(1,1) FloNama FleToxt ws Fe 12 1110001115559900900000182HMoeooOorHBBBaaannnkkk 111000MMMIIINNN PPPFFFOOOSSSPPPOOORRRKNNPPPEFFOOOSSSAAAIISSS 11I00011i55o30h00000000d88 TTTOOOXXCII1ddssSe., RRnBBLLBI1O0T1LasR0LsviIpva0e0s0BOB1a1anIrSkEkEI,.,SSDEEEE 111000MMMIIINNNPPPFFFOOOSSS PPPOOORRRAKNPPPFEFOOOSSSAAAIISSS 55 iiiD0diiissssos00000000nrn TTTOOOXXIINi4ssS.RARBLBBIoILLaBII12soo0OOoo0poee0II111).SS:.SSSESBEEEEE 1J100OMMIIINNNPPPFFFOOOSSS PPPOOORRRANNPPPFFEOOOSSSAAAIIISSS wi 1110 12 111000115050001011s10TOTTXOOXIXIdI4SSS,,, RRRBBLLBIIOOILISaBBI2s18o00O0o00o00oI00n11111,S..SSSBEEEEEE 13 101500013TOXIAS, RBLI78oOppIe S1:SBEE TOTOOMMMIII PPPFFFOOOSS PPPOOOARRXANPPPFEFOOOSSSAAAIIISSS IOMINPFO PORKPFOSAIS 11i858 111000111560600000111485TTOOMXKaI!h4BSaRRrBkLTIOLI.SRO10R0005S01ZC,o3SnE0vEMS,EED1y04t13,59 11100MMOIINNPPUFFOOISSPPPNOOOAAAPXRXPPPFFEEOOOOSSSAAAISIISSS 71188 1110001116559009000111070TOTTOKOKX1I1dd4sS6.,RAATTLTORLLOII-30MO,RDDOuyYRtO.,.CCCDoaSnontiSnrr3skISSEEEIEEE2111M0N00711133,3.993008 11M0MI0INNUPPFFIOOSSNPPPPOOO-FAAAKPOPPSFEFOAOSOSIAAISISSS 23210 1iI0DD1ii5sso9s00000ez2)s0TmTOeOXXoI1w44GGBiARnBTkLLISORNOSSGES2A50M5,pDoyC,OVC-oTn1iTo,kSSEEEE 101398 111000MMMIIINNN.PPPEFFOOOSSSPPPOOOARRANKPPPFFFOOOSSSAAXIIISSS 33315 D0l1lS5ssoso0oo00eazs4s TTT0O0XX11144465GAARTTLLToSrR0UOaSzaEG6MM.,RDDDydWe.G.,CCCoooIntnnrkkESSSEIEEEEE111M0001/1313.0399990. 111000MMMIIINNN.PPPPFFEOOOOSSSRPPOPOAAERPPOFEOOSSSAAA||SSS 33507 101001l15i6os90o00o0ee57s8TOTTOOKXX!1I4AR5G,AATTBLLSSRLGIREI2A5OG0M50.D0DS0AvC.1,OCCVEoAon1Snt1rSv2olSSSOEEEEEE1M10031.9309 111000MMMIIINNNPPPFPEFPOOOOSSOSAPAAORPAPEXFOPOSFSOAASI'ASSI..S 3331 1o10i011S65o00e90c00e031s0 mTTeOOXXoIIw44Ss,SnSSkRGIRZI0MO.OOyYS,.CCoo2nnrourk0225500M0000MNSS,.D1111 10M1IINOOPEUUOSIIPPPNOOON-AAAPPPPPFFFFFOOOSSOSOAAAIISISSSS 3330101110601106600300000556428 TTTOOOXXXIII44AeSS AS I TLSR MON SSADG OauVMt.G CDS CoyonrtS2 O mao22k85e00M s00S00. 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WietFie Botte InjectVoume SamplaType Conc 21 tiooommmnnatiioossss o9or1a 1110000000%0 --== === 338 liiooommnaaiiiooossssss 233 7 ionaioes 4 i1i00o000 === 100 = :zz= 013 iliomomeaniioidsecms 78 1ii000000099 === ==z 2 i12 liiodmmmnaniiiooossses 8ofo 111000000%%0 === =z= iiiss lilooommmneniiicocssssss i1t2 111000000%0% === =zz i7in iiiooommmaniiicocssesss 111s83 111000000%%0 === =zz 232 olioommnaaaiiiocosesss 3o1e8 1l10o00o0%m === z== 5i2 liiooomnnmaaiiiccceessss 111789 i1ig0oo0 === zzz 2Ii iiiooommmaeaiiioccsessss 22317 11100060% === z== B33 iiiooomnmaamiiicccseesss 232 11100000090 === z== 335 iiiooommneemiiioocssssss 3a25t 1100000% == 100% = ==z 333% olioommmmamiiioooessss 339 1110000000%% === z== 35 iiiooonwnnnniiioccseesss 322 1fi0oo0ow0 === =z= i @& iiiooonnnaaaiiioccmsesss 233 lii0ao0o0 === sz= 4&@ iiiooonmnaaeiiiccceeesss 33a ii10o0o% === === &&8 iiiooommmmemiiicccesssss a33 jji0ao0po0 == === %82 iiiooommmmmaiiicocsssss 33% 111000000%%% === === 8& iiiooonnnnnniiicccaassss aa3 11J00r00:%0 = = =zz 8& iiiooonnnaaaiiiccceessss 54 lii0ao0o9 === =z= 8 iomaicss 8 tao = = Pagez PagePostio2n.1:) 000711 Masiyns -SampleList | `PSramepldeLis1t0cC161:1,5M01s92o9u8A 5paaSroASS amgieLDB 0Y 15698ASPL FloNama FilTost 88@10 1o0io15l5os8aw5dm0so8:s0 TTTOOOXXX1II4dd5ss,,,RRABBBLLLIIIOOIOIO1D%1S00000020o8101101,0.01SS0S1EEEm.EEE 5 1o0i1s5e0a0d0s0sTT0O1XI4d8s,.HRBBLL1Oi1O%-11%00007p810.10, SoSEeEEE Pages PagePostion: (1.2) Fe 111000MMMIIIPNNNPPPOFFFROOOSSSAPPPOOFAMOPPSEEOAOSLSA1SASS 1TO0MMIINPPFFOOSS PPOOAAAPPFFOSOASS1ASS 200712 Masstyns-Sample ist. i Pr`Sianmpcle FstOG15 A115M81s26oA96u8praSl prSiSamLpieOYBI01N6008N .5PL IetPle Botte ijectVolume SampieType ConAc &&@ Iitooomnneariiiocoessss 7 o ii10gg0oo0 ==-- ==- 8& iioomnaaiicoesss 110 11005000 == == Pages PagePostion: 2.2) 000713 FACT: DATA REVIEW SUMMARY FORM a - } ore --LXeZ actsna argos sei _PEPTTIR. mE en wes Lyli DeAGE Ss --ioe bye HDD=a O de oe: 222%0 bx _0.Gr0F `MBoanictopnBelankoJf LU0R8 DataReviewSign-O pw-- eno wep NEE ar spfanfes|bhlls Nquoi pmraeReem srs joLAClalga [-- _ Quuu2al%owterideaopytnuttrieise1. 57l% 5 2 7 . aes -- ok [Catibrsion Check[s:2% = =z ZH Lavanza Suess Seace LOM en) Enkred 10/234 we D- ESH coismrane 63 SE pols ks nT Corts DEED TE 4 Nesey [ TATC dniEMS E 13a/39. Loind Tomesse sansa) hoe 93gol fo A2077 I 0K on Checks: _ (JK secs [0G SuesWOE Rute us gal vefam l0| tescomand tues ma stogss -- : ny) Endod LAL [ol3a]94 hour: 2006 (3) > [Acceptable LinearRange: ee ------ oo oe Extracted Bask __ PNr E sac. -- -- MatrixBleak: --_ lows sone 2/222 0 2202200 LolBas Tutor Guenttiors 000714 _ -- = =es i -- FACT Study#: TTCJ EnL B p SEdA p T -- m jeewu--iluilnwn Duchnuit FACT: DATA REVIEW SUMMARY FORM Poh 2% DataBarry: 10la2]99 Sur" (@ ce LoowRange TT Jroe: up--~ oo 2: _ mem---- ExtractionBlank:__~~ MatrixBlank Sa p -Bi~ r-- [DataStatus: jonChecks: - Sas --S------ -- oe ; -- 000715 nm e BmE ED . 72%, - %% iIEE SEoRnEEpRiEELEsEE | LL HOEEmEE "obaly + or iol rb ig ho, bn kh lh and SHOTi0m ry 4, Sey ue ofa] 000716 TRE. oo o---- to0ottso | fig BREET FAME us. movseny 1e. 3mmin.e, 01100 1 mis [HE ELARPR R HOE BE WR oad an ne aE kid a > ucJas This Grima number ney hore ban swlchid ith animal AR03250m, Dat, sky tolaz4q | 000717 Dob nob usd, nok a target anchyle Lr thie hd YC tofaafag 000718 lo orm(7) Dake noobk wiuid 8 ln 00719 BEST COPY `AVAILABLE RCaooroantifnonecTurhve: 3o5m1s3S1aa+Ai50r9a83958249487 urvseatyipse: Linear, Origin: Exclude, Weighting: 1/x,Axistrans: None. 22225 wh | J i ,: : / . |J J Pos L % 500 Wo OD 1h 16-5155 5 0D Sos 000720 BEST COPY AVAILABLE ET ow -- FT: py --: Sm, T:nFedSen EAs,Weigh1st,Aixinstgan:s: Noe i | J/ oi 7, || || || y/ | < Te a en we ORD) 5 112735 000721 [F SopRorteetFysapcP :opEgxt3oe0m0al1nS1us,Arees Re | sas eee emmmerreteam i ; 2| | sod | | i A | pe Se osr mw er es ee er ws es oie m OBE oN rns 000722 fe ee T CReiemanbmrR:eBerm Su,Area | ond | Resp oi | / i | / i || / / . i b [1 ee ene eer we isConc. @ 63 hor wins 000723 Erbin EC tra pe ur we gee. BE wo ER i sraelio suszop ws 000724 puloaa AV 255 0007VRS lets aT 0Cc0726 . fnslis WHY 3005 000727 : 17% QAuMeELrI,As0o6u24s98: FACT Equipment Log 99114 PAGE DATE, 1001989 ---- STUDYNUMBERTOXISS_MATRIX: tive DESCRIPTIO8N:1 FACT Matos: ET5870 econ ohm: 100 Cy 10 l Bcd: Zp obser (omucmo (eon Potty TNors- todrA dmum- ber-((A0) 1900850-1605a-194. - uno Somumontm? @ or Nove O @ wImNaA2S .0um0 Guardcoms: Neos Patounberss Lot smb J ---- sar 250m pecs Spb sm 33m Panoumber 557012 LotharPEL Sealsanber 5918167 Amy presets: Dogsi(L3g00 Steps(Llo)J0__ Sea LsA . LCFlowre 0a Backrs2s HPvesLt raCdios 10min1095 THienee mn "i0n 513sm 5o5o0 01s 250 sem fr 000728 BEST COPY AVAILABLE 2 ning Method Report . rage 1 sathod: ASSL NELIA-RATS. FRO\KCBRRUOSDBKAY Srinced ue 0st 15 10:28:49 1999 " OT R080 msEd TRAE Ce ea S hn Ta . Fy 3 soma xar- ) set ma wr se nan FScahpritt.ary & W 160 NLs i1T3nonhRaeSssaeoatluutrtiioonn ii152.30 o SFSueertnyeemotck Tteomm. #a14825 153 pWToenlstEinpeiregry Ramp 0 ihot f-- na Anatyser vacuum 120s Gas cont ses az iSinarRtaessiootdeuutriioonn LfIeoonnna'ESsnhearrgay ramp Wiintispiier et nan 1H100e.0 wn WBit a QGo B 000729 } PON -_-- 0 77 000730 Says SamieUt Samo st CASS asPROSATIOOR RRSP Printed: TueOct 19 14:1027 1999. ovPaiget 4 Page Pon: 1.1 hn Fiabame FilTut 3 3 omen wwoene use IQuNPEROSsPPOONSEEpEROOSSA 813 11II0SR00TE0E TTTToooorurlrnR issRRnaBUL1I0E1S0L L2vhe0obata)CtCESaES xSsLtSEeCE1I01011153E008 aIIMOMMUnINNPPeErIRTOROOOESSSPPIOOAANILAIIEISSFPPSHFrTSOOOOSRSAAA f51o 1Tl100o10Dr000e0 TOT TTTToookroI1AiSSSsRRRRaBUEaILOOlSIIoDiOdpIoooCIlEEiEEUaOsSSSoEEReEREOIIa0baTL11s30t!E0s0 TonRALIORI11 ESnSESE1010300 IiMMmoiuNNnPPErRrEoOosSSSPPPPOOOORRNAAMIISSSSFPFFOOrOORRSRAAA IouIPFOSBOIS SFOGA : H1BiIEd0e ieeminETdTE ooerotRlieonOaazIsR o0u0S ctoi nSE tiSsEeEni 10.1390 IIIINOOePUUErOISNPPoPLOOPOPMMsMEIISFSSrTo OPPOEFrOOOSSSSSAAA 1 joes TobG tSTOo W.D1 IMNPOSPOMISPROM F B J 3imowI meey isTTT ooidE owreT eeDo nnASR LReTABASA OBClER10-199i9 IHInoMuNnEpPErPrOooSsssROoahmvAoOviiSSSFEhrrOEoosERkh BBBBoCombEw S TTTookouSlsIs e ORONNum BDpm uoLksa sSSo rrToOrAn Sau toOSIitI0yo IIIMOIONNMMGNPNIFrrOCOOSTSSRPTPOOOOMWMLWISIESSPPFiFrOoOOSSsSA B @BB1Ioiv0EEi Rios eTTmoorotniike imRRLus iooue nsliieonEnsoSeesEnor|u10a-r1008 ToRLOSISIL IEBI RE IMIB MMNNNPPESE IPOOFSSOPOSS IFSMOPPPMS rEFSOIOOGSSSAAA NIRA TIOS MBB 3 OoIRI ioE 35 IE R ToTESsI RR R elO LiieO IsoNc iEINCeIeah lSsSe EeeEt1Be 0es I1R3hE0"0 To Re od 11 CSeE nie DaIOOMnIMeNMBSrFEFoOSSsSPPOPMOOeE MMbPPTrrrOoSSaoSssAtt IMMPEOSPOM Prog 333 35 TOoTCHrTE TTR Toooxun H14a3.I RRRBALLBIIOGIO LNISSII0O0OR pI111S1EEEESSISxSSE1EEE0I0l0i1E0.v31eS50s0n0 OIOoUuMRnNPeFHrEOOoSSSsPOPrOAOoMRNwISISSFPPpOFFrOORoSSgAAA n00731 Tiras mss Printed: TusOct 19 14:101:92979. roeT=H Lb] CO ea arrose eons? oo FACT: DATA REVIEW SR SUMMARY FORM Foge A | -- A ute | acts TEAS -- uly Masten: Tall Lie (oe = od holy Pity TNebmserOuRkefv : fanos sie fozeste fQouttyoAssseRew . Sai _thaley 7a Duta eens UC lolaslsd : rt tual 0izim Commer sath: re Tone a Iahl8 phy tod zoel ] eo det l fmt saie =~ plopFt adetilugs desde oon n aypd-- ---- t[-ing TToo 3az, p wi-- rasee:2s(ers). lon uaUenlepuke trend iF. = Tas Plufey A i eto tog: _L2-pl > So7 Conde be Porn prs =Tos halts : oa_r.s5p sev fondalon| Te ptuipihinedoped Ira _0t12022. desesunk fosMunf00| from Hocuree mo Hat He Loi Bl neu [cutbeuton Checks: 4/4. lows sue 2 4 | wreld be ose Hor Yo Log Toe in orden | base E _ 72Ecc)? ree hoo _sa [Roa Sr MatrixBlank:_ pA Dinea ess Haut 3K ETulfofy ! |p tea tallfl saa.. Td e 0e 8k hev da ion f 2%. ~ b[ssmso_m__ Chmke mls uc olds bo tho Bt 6s o' hol --Tnc hay [Dats Samus A - 000733 . FACT: DATAREVIEW SUMMARY FORM Page 2/2 onoe u SZihh DataReviewSign-0fT + NAsinveerTestfufn oCTswys Totwsoagly vs llelt v fe=i cub,Pol: es Puy TvehmiaowReevefhe bopogy12wctela): swe zste QNuielityAmsrusce Review Sane feces zs LC ST mee o neharate Gr ttt oe Pros Coef foTmba.d. B end Js wT ser %yy Temlop oking Safoplm=vpnaralfodt| fib aL ffear lHeaedHo - [2 _0ffbofz vain Bunk fos hasLoa ol kid -- ze nd Chocks MA (2)yLots xmmenscem=oiiiea Dats stats: Ma a: cept in Rg: hoe - concen May er dete Conse DIE 10=3), ----eee miee Checks: Mss: pet * [Duta str. Y yy cat bow Moe ---- - 000734 000735 000736 00073, ST Ieam . PEE GEESE BoB BEE 00738 BEST COPY AVAILABLE SHES ne fi er Pomeeone e | ] eke, Weighting: s,Ai an: None es fats 3. LoES i | 4 Resp || J | | Lo 000739 BEST COPY AVAILABLE RISEN rn ve C C lciurve r 4e 468a37+o1e45n .n65m 2 C | = eEe Sapa:Ext,Weighting: x,Aisrs: None | | | Response :| J/ i . J L| o - 00m ame emu whe ewe mmm 000749 BEST COPY AVAILABLE Cpr" At Earl,Ame | me. i Response. / ; J/ || / / Se ol re mes we er es wie Conc 000741 BEST COPY AVAILABLE C Cllbratoo nocfuBe:e1B r75m980 +x0+357660687753 To Cee 2 TeGom ng Et, ude Weighing: 1s, Axs ans: Nove || }| : J |i / v to o FO NYMMA YN TT Choene 000742 el o me fel. OE EE " fm BB 000743 pen em n EN i_ 000714 EE. BE mare ud o ATR = ELC l sain. = 000745 ] 000746 AfMELaLAt06a248: FACT EquipLmoge99n11t4 Pace A rn STUDYNUMBERTOMS MATRIX. Babi LiverDESCRIPTION:Gave PACTMent: ETE470 tonven 05 Cen 10minBcdZS onroe (pomomonc: muon EA -- Emam Ferhehy Es Prepared(date/by): (A)1021/99sab (8) 1020099is `SExpoireesu: mome? @(Aor).04N100ose (8) 04100 Gane ---- Lo meen Anitmea KsBen Surzxtom a -- rman SE Anat arses Drs )200_ EstpuAi010 See sam Sotents Sheng120 LCFlowrate:300ul/min Backpressu62rbea:r HPetLrtCs mi1095 bTreee ="a aor0s nwes 4pyr 150 oo950 000747 Tuning Method Report : Macho. USSLIOCVELIA-RATS,FROACTIOD\pFBL 6K Page 1 srinted son 0st. 25 10:13:58 1959 iA Era RRR Ci aORTte RCE ciL | Ha ; 5 F5S f Tree m e3 . R oe RRGls holt Ba SE el B60eri KySU REED RI somez (spe) fcEaxeplysftacienoery SA esefiaamaetion: Temp ser max eW 1o 60 kLeo H $ 82 m H wor i1TnongRSaanssoeetbruuoccyiioonn WiTolnntseEanteireery ramp sa mak a215s%.08 i$0so Sresmures sank Anatyaer vaca Laemt Gas cant 0502 sz Fi1fonant'RRreEeisrsieoeerlisauuyteiioonn pLIeonnea'Esneroy ramp Witter ser man 2111%08.0 oaWs no so x 000748 000749 Method Report NTaacnhootriirdiee:a, Petated: age 1 Seandray.\Smekoabersi35as,ca1lt9s9.9ypr1on0\1u1ec3q0u\1b\1soaaia-a10-l35 Monday, October 35, 1999 10123144 P1100 LC Pump Tadtial Conditions Solvaente 0.0 a 3:5 FSHilteoowppTr(eiesm1se/umrie(n.n)in(eh)ar) 20o0s0.3300 VvSavexennpTTreaemmsppseeurrraaettuu(rrbeearL)Reitgtht((C6)) 03i0x0.0 P1100 LC Pump Gradient Timatable he gradient Timetable contains encries which are Ti7$n10e600 03a000 11000000 eoohio onoilo n501o538w000 Preoosoosure 53a155300 2501203 33i50s0 oole8 ailae 222111333666000 p0ri] P1100 LC Pump Exteraal Evast Timetable he Timetable contains 3 entries which are iTir5n.ie5a0 ColSumeottSviteh Coontonaicel ConoStneatct sie ES Ste Ste CoSnetSaetctts Set ContSoEaaEcts Ste P1100 Autossapler Tateial Conditions DEBrjiaeacwsesppSoeoseridetdion(u(/omm)in 2250009..000 VSInEojTpecTTtioinomenb:eVr(onliunem)e (sl) 1HH050:005 Many -SampleList SBaimrpoLMiosnt GCe:rM2A5SS1L0Y-3N12mo10k9a0 ratsPROISanpieDBI025086SPL Paget PacJeoPoston: (a1. n FloName FilText us Fe otFe 251 i1100032z35s09o00000002a75nMMeT aOoHhBO BalarnkkX 1022I 0420BEarkR,10D 2290RLWW {JIOOOMMMIINNPPPFEFPOOOSSSPPOOOAAALRIISSIPPPFFFOOOSSSSAAA 111000mmmiinn-111000.9.0585.3 313 11100033308000000005038 TTTOOXOIXAIBXRABLGIIRCE4DZDLLG-oiItvpeoGRBmm1wE),aBIISkOZS,ZILZN0RGZIQ2RRUWWE0WWRWW IIIOOOMMMIIINPPPFFFOOOSSSPPPOOOAAARRRIIISSSPPPFFFOOOSSSAAA 11i00mmmiinn1i1000...088553 137 110003m3000e1s: TTTOOXXOIIA4XGGRRBIBLLI4IEESGao10oRpPbooepBo,otIIL1SZ0IR2R0OO0Z0RRRWWDWNW IIIOOOMMMIINLN.PPPFFFOOOSSSPPPOOOAAARRRIIISSSPPPEFFOOOSSSAAA {i10m0miminnn111000...09555 01113 11iG00a82s03e30e00040i86a TTTOOOXXIXEIIRSBSLRRI8aB00G00E0E00Le0zL111I0,.IIIISON00E0OGZZIA0NNIoEOORRmNSSMWeYWW IIDDIOMUMIIINNNPPPFFEOOOSSSPPPOOORRAANKIIISSSPPPFFFOOOSSSAAA 1110o0mmminini00098s55s 1 TGE0047 TONSRBLIOZES 120IG0Z20pORW,W 1OWINPFOS.POAAISPFOSA. 10mn10.5 \ 000751 Save samp i `BSamepleLiesnt:CS AMASST LYNXaR melis-atsPROVSampieDE\1o0z2s500%0eCs1 SPL. sate Volume 1iis8wEw 88 i P$[I un 2 B5 Po n ' ii Pooez on PagePosition:(2, 1) 000752 ' FACT: DATAREVIEW SUMMARY FORM ' pay lef ) ou lt [I -- ee fre J OnaAnat: Tas. sMernsf"Tnpins: TCfhaaotue=ddoise cocl=iohln. SIA by mefees wnt Sun ou , ganni "NameDue: Ths letfir RQouElgyEgarioniaorff[bp tor ountare De Repocked @ pu uff? Sm Ton PO Of aptad orp came i ba ee d va o m n?e|Riadedsgooid opweon4 4d here Co Lgedue Lpath eAis be se Piss Cnn N00 Nm boo Lag crt hordonw 25 SLTTT ti A t oh on Tas hfe Or .. 7 plpk-- vd p ve 0.099345 Mazi Blank _ NA Cooke fheFuge oom LSA 0 5s Han23% inHo per hu fie od a| Spe fire Ton cor P bfrecome z mpl--g [op-- een Oos c p ffp uotoe gpplyr edlitd k edae Lensf bnarrdy Er Jossse aFsiescaaac= hdlaeiedsydfhoeeldLoer Otwa wee ner kusdH Tis Die hwsil dd E52 ps wie ser fl 1eatheitred of te spe gue ujsfyy "00753 one J cw gi FACT: DATA REVIEW SUMMARY FORM DataReviewSign-ONT + AostuRetr pweeg 22 ulorlat ve [ ---- - | eacTsyr Tee TaintRover \ Movoto TCooonsldiososad--ieeliWanfty NamalDu: sei bgt seamhniia NqoesnAemeracie Ones _fofuftt neg/fs wate oes 5 Commas emm-- Sr O1B won conde int lonkd Le ord i LiouRangesA Ta=carves liad acescroandn s lic SS Ma begin Aplin = | Sigs # Aa fecha] Serve' Talislufry owe Pate Rng: oe: ea N---- hesresn tn Bl: Mase Bc > o ; ous sume TwA isolialipedsmieodsn we s 2x fe dil] he Cophachels_earand sdf Le seal Tetfmfy " Eto MaiBl: 5 : SD 060754 200755 RTL. setrmrotsrons. om --- nd mesg mE me 00756 : 200757 000758 ((CCooino1cdufiarDnvaaemtr:feo7:m.3aiPc5nO1aA8tt8Aen+(:xiC0+hn.151)o006t.95n58814 : Fsypse:yLppien:eErxo,ioOrminagilnS:dsE,xcAloruedae, Weighting: 1x, Axis trans: Nona 5: Towifufoe 4 Ltt | / 00 ihg abo sabe ado soe eoho oho Cone 000759 -- eeeesse -- ([CCooefmfpiociuenndt o2fnDaetmeer:miPnFatOiSon(:G02.9)99345 [(CRaelsibproantsioentcyupre:ve:Ex8to5m.a8l2St8*dx5, A+r4e0a.9705 (Curve type: Linear, Origin: Excludo, Weighting: 1x, Axi trans: None | / | | Response . | | 00" "71000" 7000 3060 4000 5000 Gobo 7060 Cone 000750 eeeeeeee ete------eeeeeme ([CCoofmipcoiuenntd o3f nDaetmeer:miPnFatOiSonA: (0G.9H9)5986 [(CRaelsiproantsoentcyuprev:eE:x4t0o9m.a1l88St,xA+re37a50.14 ive ype: Linear, Origin: Exclude, Weighing: 1x, Axs rans: None 3 / Respons 00" 000 2000 | 3000 4000 5000 Gobo 7000 000761 RR E o ems ann [Com4npamoe:u IS(nChd d) ; J 000762 mmm - \ 000763 000764 -------- ee ---------- 000765 _-- FL 000766 1m92a5/g50 QuaI,tr414o5. :Log 99S0O92UP020199:PFAAGCET 1E/q1uipment DATE:loz899 SATNUADLYYSNTUiMaBe ER: TOXISS.MATRIX: TucensamplesDESCRIdPosTe cIntOcmNuio:n FACT Method: jectonvolu10m4e: Cycleime 10min,Becrode:ZSpae MobilePhase: "nomNmCmcor MoH = TNAcrmidonbr 0)SBS Popued(hy: (WARUSSmmh BINAZNY @ldsmomn Expl: we mame Socumomung? or N omer CoNd ow on Pomsben Loomberm AnalyicalcoloKeuomBeasl Swann TweQll Spewssesum Poche 0SSI0L2 LotmberSELL SeismumberQioliz Analytical parameters: Dryog as(L350 ESTebugals(iMLnSg Shgeas(aUhth LCFlow uien0in, Back pressre:b_aGc5 HPLCsolvest gradient: 10min10.95 Thooewn o sm o 210 wo 1P5050 ssa me sas we 000767 BEST COPY AVAILABLE `Tuning Method Report : Macho: CVSSLI SOTP020153. PRONACEUTBAPFOS3 Printed: ou oct 28 15:2013 1999 rT ZF | 17 EI +0i +|EIS A- " | 19/25/49 MEE Page 1 SARA a Somer ( zs3- ) GCaoEpxniEerlalcacroyr DSeosusrLicevenascBiloonck TTeerrmm.. | Co Er R ser nan FLaeo315s F501E2s wr L4TtonRRoEensseoorllguuycciioonn LTeolnntsiEpniesregry ram e-- nan Analyser vacuun cess Ges cent se sa po1ttvRReeersseoollnuuyttiioonn LSZoeonnna'EEsnneerrdgyy Ram Miietispiser se nak o11l80s'00 Fiog soo see na o i1.0a &020 a PA s 000768 _-- f mrpi, retavear ee RnrRe m-- Thursday, Octeber'3N, 1999 13iediee eopee -- aW2w 5005.8:00 2 3 rr0Eo3werBtiesuCmgsinn)Ea]e) 105.3300 poon Imma ne, 56.30 2140 10 em Graton. simsanie [fais The gradient riTmhe Timetable al concaing 3 2n satrien which aSo ace : EI] io i P 88 oP noemw veW8aasguce iinnoaoan 26888 baodt FGGee R.gaa1mm iiis 11300 16 om carat re Hmm he Timetable concaine 3 ries which are HT4oe8an3 Coflsmipission ComSt&Giaecct Conp&Gtaieerz comSoaiciast conSSsaiaeses aB EaT e p e amie 1B0H05..000 InghetRionEVoi ga a1000 000769 13/25 fgg e-- e -- ee ----------------me-- e re-- ---- pipelined 000770 12/25/95 M_a--rs Samia Lt rMe e Pant Srb aieLintt CArMASaSL1iMi onupt2019 prSamb palsee.OEBPL s) Pagepsn: 1,1) Flotame FiaTert ware 11i 3 tlom amk0en eM uFonoreoe wms mieeEeo sMiatomw, iANsY I 1II RAoNN EuE SSnITsE OpS oONmNrTEPErERS E oOSOSSSSAsNISS lim $ re m EF E F S O a e Ir Goe eI y nni sia ous sts toryy IIITMUNNNEPEPRREEOSOSSPPIOOONIMNEEEPRORONEOSSTANNSSS JTBiog IRidRRaFrF CE MeoynS eiO thSSaOI unnReidsE iiooS gs mrhtwy IITOOUMNNPPEEEOSSRPPFOESOAMOSOEIONNSOESN INESIONEESS . T11i53 iiIcoo2ka0mmmiiiss3 Em TLTo eoOexXl anEssSPPae IanEsETTwSiOeOeeInn SroSGseTedHtPoOotONS10H2ANE8YERSARASS IIIOOMOUMMPNRNETPPEOEFSOTSSPPTPOOOOMRMMEKFEPPETOECOSONNYA'SESS 1I1B IliGmiHasemDisa Bi TTLF OaRnoEehrMo oasAinRTHvSu ouenwcPoorSsAOn 2E00P0S0H:S1i02T0nsR1S II TOONN PPRFROOE OSSREPEONO PEFECS OrNOSSIAYSSS IMNERSSIONEROSS F EBliiRaeannEs:a o FF rOoo Lt oIoIomRm onos ETH1i e e0OIe mRd LwA ITIINOOENTUUPINPPPOTOOONEMMNRFEEEEFrOEEOOSOSOASASSNTIRESS B IBii e ceFr O T O e o A o oom oo nSI e tIae nTtmAnoynrYy IIIRMANNEM EOESSTPSAF OIAMSFENFIEOO OSOXNSISSS IRANEESSIOIEOSRS BOERS RNR eS a TONS FOMPFOINS 000771 EE SaaterlLs:SCaUmpAlSeASlLoOtOKsuptz reeds Printed: ThuOct28 13:441:90909 8pSaTOOBCEO PL Wie Bot InectVolumeSampleType GoncA Gone Conc 111 ofoteommmmmeeeerss Bi Bw=z= -E=2R zo = o== 8 ji lmlcmsnmeitesssl: BBBBzzzz =EE2EO = = = = Hh? oolmnileemmemss}e BBB8zz:s SEER EE=oz = = Srumift ii*Boolmmlieeieeiessok & BBBBzzzz Z Sozzz Z o:oz B#jBloaalnomrneeisesss w BpBBi zzo EEEsRR E= 2 - 55LBomolmemiieoed3 BBBBzzzz EzzzR zzz === iBBBooomddlmoeainisni BBBzzz= ETz EEO oz D zo= Bowmen d Bz zzz 1ol22)99 er foParge2. PameeRA 000775 AL ; `Fact: DATA REVIEW SUMMARY FORM } Date Review Signo oer Ze WR edy FACT Suty [I-- Ts stub {le dy2 od fy - ex _osygs rn SINT Ze ous me nd airy --_-- weterr_Disliede] Gan wis) -- 9 -- OTFanpl api.` " loc Lioeus Range: [ sph L e-- s [-- -- cd " Me "i beadddfk nl 4 [CubanChecks: pore np =Loge fn 7 57, -- 4 v / es sune Disk nt Upred Guild aga elit Lisoml -- roa jz. on ve __0my BarcudBunk:_Lss Alenlpg Mssre pried flanley [un Cor 0Zang foe fo pry srs. _7% Aj es se Dig bf (ule) pid veo US| hase See, Ccas Jooeselhee"cte Fr sa s[aaibcrsasoio,n Che-- cpksr: lose ot cu Igo EntBn _s ema 000 26 ball Hor A hey [fod His pond.495 cedulidfom He cine Hot beensy tro. "Lo, in Les [Em " a : as Ylchy N pt _bee--e be w -- fc ac lLel,m y e Fosthshy pod 000773 FACT: DATA REVIEW SUMMARY FORM a Data Review Sign-0T EE acts Tugs elonTey [re -- sowie Belle]|dee eg v-dh~ y pls, N[amerruepRb bo gone| Qurasa Boyker hc ufonlas Sap Anis New To Duckie _Tas Yhisiy pr Aeds otryss tae _reli do pag Pr-- AC u/ag)91 5 ww Basacent" fos fad pine Gpiod p20 o foe 2 opSE Ex E LinearRacge:_30: -- J ol 2 fe - .L ke otis Mar Bioess2) th > Chocks: OK Fange fro poren du _o lb [5% --= 26% (hah) ay / lo se_ox Ria Todo Rooted Fe 1 Fad ae cept io Rg: Joc. - [-- Mas 3 [Calibration Checks: i [DraSusur Past: cease Range Joa [R2: SA i" sss Ou Gu ft Ems Matrix Bask: \ I A -- -------- 30 they . 242 000774 = . Zou tley - ace. wm Aras Gone. Fiuge Vow atComms 000775 EEE ee HBEiE ES E s EeCT em wn THH E oHEgRieweenr 00776 FnSET : EE i] BOEou 00077, 000778 $ C(CoCoamcftphcoaienntocfmuDaeetm:eer0m5Fi7nOa3At3iR6oTn3C: H0.D+98= 911998913 CReospeonyspeety!pDei:nEcxat,ergmia : Excide, Weighting: x, Axi bas: None: 13163 Fae bey 5. )-t _pe ~~ 165e , " ee 1-%Cone i as0l1)090020060 abo0 e0d0b0o swe5 i0T660600 11330000Cone 000779 Em ERE = "ECRomeyuEplh"ocSeauoimrmE eee:b5r316r80S i2+boe36a0rn.a801 - EE ClivLeispees" Linear, rg: Exod, Weighting: 1s,Axi ans: None ~ oos r" . } im | res i | os- ,1 ~ :4 ----- fron] - ----t2005___0 300 who7 ehoTTUe RD iieoese iiaewsncone i "0750 Emme -- mame: Sur| - [rte Bp a fm _ I 4 i i [pn | | | || ass | Sol 000781 [CRoeesfrpfoiacniisoeenntcofuDtype:eeterETxmteir2nnala8t5io5n: 0+981938141501 Comvepe" Einar, Orga: Exclude, Weighting: 1s, Axis ras: None 2.1364 | _| ~~ ~~ -- |! { Ln | ||{ : |TM BR 1. : -. | i i | | eS R aew abo Gwe wR ho ioe mwo 000752 ERLE AEH amar eset vm sosose Eien HE in i fm mH wad He. OE Fr Tanvhily_p lett - 000783 tree --- frre BEE oi Fd . Bb 000784 a, ER Be 000785 IE Fe wap ghar Eh we or 000786 . See srg uAMEaLIitAs06ae24s98: FACTEquipmentLog 99114 PAGE owe Laser ANT STUDY NUMBER TOXI45 MATROX: tn DESCRIPTION.Duy 2comedtoe FACT aos E15.870 Ingenvolme,_10ul__ Celetime 101i Brod Zr Moen osMMuouco: (Men Aorsmdamber (8) 08811 @meazn [oSeoran? @ wouN Ober 00 "3 Prepared(date/by): (A)10/30/99sab. (8) 1029/99mee Guard oem: Nese Penns [-- Antti coum: Kes Bes Sur 2150mm ects J---- [re [---- ---- JRr-- Dogs(nUA2g00_ ES nbps(n LAMY10_ Ships(LAD ; LCFlow i 00uLin Backrs: 2c HPLC vet rains min1098 . Thooeeain f"iar hhaisme 2w0o sa 10 "COTS? BEST COPY AVAILABLE ded j-- CASS `Tuning Method Report : NELIA 2458. PROACOUD\PTO6BK1 Page 1 s-- re ancee-- e-- Fesceov -- 05 09:42-- :00 1999------------ Yi cSc R 2 Ti pr Bi ATE e ahe A rfh el Sa : sh LEN Eo somes nape) ser na a sec na aEcafeatcieaorry fey FWLIwe LLge HA Ti0nonRRSaahsseotrtauuyciioonn ion Sneray name 211%55.80 os S Seuiin siock hteemm. 3330831s flointster Fia o # Pressures na saz Set nak Analyser Vacuum L200 Gas zat "02 i1n RRfeeissooollmuuctiioonn fon' tneray 11100s0 2 pfioornnisF]serd) nap FFoiOs ler So 000788 ee SET rr 000789 Nathod Repore NTaethtodonilteh,ea: Printed Page 1 CF1r\idaIy, ONovSemSberL05I,241E9989.5\PR0O6NV1A1CE3O1T3DL4B\I0mAta-G10-E55 Friday, November 05, 1999 09136128 PLL00 Lc pump Tateial Conditions Solvaaents 500:.00 aa 33:50 SNFilenopwpTrmeis/nsemuriein.min(eb)ar) 10o0.0300 MOOavvxeenrpTTreeemmsppseeurrraaettuu(rrbeeaxL)Reitgtht(+(c+)0) w33p0.00 P1100 Lc pump Gradient Timetable --_ The gradient Timetable contains entries which are Ti3n71e56 S50a0'00 118a%00 aao:l oxon1o 02l.:o33w000 pre55s0cure B5a15 iso 555:01 5505800 [xxo3]i oxii1s 0521::3330000 i0aa WPLL00 Lc ump External vest Timetable The Ticecable contains 3 entries which are iTii3m5ea55 ColSumiocttSuitch ConoStneatctl Coontonarckts CSoniotcarced ConoStnaeced a3 Sit See Ste Sie See P1100 autosampler Iattial Conditions - DDeercaa: sppoBserieetdeison"(ua(/ommi)m) 230a03..000 SVTtnIogApectTiimoen" V2oilnuemre (ul) 31%50:050 000790 Massiyns - SampleUnt - Bd E List CMSASSLS YNNaE mata082458PROISAMA0BN105008.SPL i Pe posior ) FileName Fil Text usFi 351 11111i00085k95090000000102MMMeeeOoOwHHbBBlaaannnkkk 3 10350004 TORSRL10i0-RWWWoBo.x91.2,11 1I10OOMMMIIINNNPPPFFFOOOSSSPPPOOOAARIXXSIIS-SPPPEFFOOOSSSAAA 10MINPFOSPOAXIS PFOSA 785 11T10i05053A50N00000007s8 TTTOOxO1x4X51RdBILs1AR10BR4sLBE1oL1mbe0icItk4RR,I3WURNW.-I.WBcB.oBpoBxxomRoxh6t8aB201.1T21L11 3 110530008 TOx1dSROL1I10RAUS.2Bo0xp0005011 I11O00MMMIIINNNPPPEFFOOGSSSPPPOOORRAANX111SSS PPPFFFOOOSSSAAA 10MIN PFOSPORNISPFOSA 013 1TTII00038S3993000100018. T TTOOXX11Odd8GRROOXLL11I110I44I00c1o0d2r6o530TS011R.0.RRHRwY0.::B5BB Soooxxx00990L065302111111 12 TI0E0012 TOXI4BRBLIIG-RSWW0:0BooxgSen3.11 1TT0OOMMMIIIN PPPFFFOOOSSPPPOOOAAANIIIPPSSSPEFFOOOSSSAAA 10MIN PFOSPAISPFOSA y ie 11i653 16 1TTT1iII0oO05sSE0e0E9a000o1N1t340s vTTTaOoXOwIOASBXRaXBnILc.1AS4RS01O02RB0000B0nRpZ:L,RABMWIRSWDW,:WLI1B.I4So9OxoI08xS.BN0IooB2eS0Hlxt9092b11IO1IT0OMOMMMIIININNNP-PPPFFFFOOOOSSSS PPPPOOOOAAAAARRXIII1SSSS-PFPFFEEFOOOOSSSAAAA IiInT 20 1TIOI0SGES59E0AA10T 110593020 TTTOOOXXXIII44AESSRRRBBELLL..GSRROORJJSOEIJMADEMBDAEMRGDSoYD..rZ1rS1A41G0191104o40B09ro000x.00-2011 TOXIdB aL.IROSaICanItolM1aD 111O00MMMIIINNN.PPPFFFOOOSSSPPPOOOAAAAXI11SSS PPPFFEOOGSSAAA 10MIN PFO PORA1S PFOSA 222011011105095093905082231 TTeIoOSXgIaRBm4L.SSRR0Ls2I431M 0De4y 0|C-oR0rWopW,.p1S1oo4x5C980O2r11 2 105900TOXIds aL.SROSaCuanN,1O143R8 1IOM0MMIIINNNPPPFEFOOOSSS.PPPOOORRRANN1IISSS PPPFFFOOOSSSAAA 10MIN PFOS PORPFONSA 7 335 1110053993003088 STTOOXX1IE4U8GRRROOLL.SSIRIORAOITEASSMOIBEINASOLT.Oi1ARDGnA.D 2 05020 TOXIASROLIIG-I00pBCO2r.RW Box 6211 11I0O0MMMIIINNN PPPFFFOOOSSSPPPOOORARNIIIPPSSSPFEFOOOSSSAAA OMI PFOSPARISPEOSA 333 m11100e5930h053 TTNOeoCoIunSsrRmDeL..I sroazsS amDR SL ONG. i 11430 J I0SI002 TOXI4SROLIING-I00spCONT,RW Box96211 OT1O0MMMIIIN PPPFFFOOOSSSPPPOOOAAARISISF.SPPSFFOOASSAA OMI SFOSPARPIEGSSA 33354 38 1T11I10G09E59E0003305vTTeOOwOXgInCcdHORIBBW4ELIBIiS,eIRrW,DRAIW:IWB:oDGBxo0Rx8L20O1211L1 110593038 TOX145ROLIIONSoop 1.AW BaxPB TOTOOMMMII PPPFFFOOOSSSPPPOOOAAAARIISSSPPPrrFOOOSSSAAA TOMI PFOSPARISPEOSA 35ST TTTIII00GSSKEEAAE00030%79 TTTOXOOIAGXXRBIILIIAAGkGES 1S3RR00355p00nEO0.011,.,LLKAIIoWiMitIIBBBGGooowexB89AA88133SS11111 10835010 TOK1104.d260Sm RA:OBoLx901211 1TIO0MMIOINN FFMOOISS PPPNOOOAAAPRIISFSSPPPOFOEOOZSSSAAA 10MINPFOSPOA ISProSA 41 G$5 1110I05053500000414132 TTTOXIOOdSRREKNLLI1111I1000444s03dASa000c00o0pSSo0o5po1ttRRRRWWUWNWA.::BBBoooLkxx0e003I.55111111 T0SI0i4 TOXIAS12R0pgA0tLRWIW.IBOXOOSNE1 TTTOOOMMNIIINPPPFFFOOOPSSSOPPAOAIASIR.ISPS.FPPFOOOSSSAAA IMI PFOSPONISPFOSA "0791 Masatyns SampleList SBaimaisaLiFsAtNCGM0A8S0SL0YN2Xa5m1e94l0a0062468 PROSSS40B10409.5PL rez Pago Pastor: 2.1) IntFle Botte InjectVolume SampleTyps ConcA ConcB ConeC ConcD ConcE ConcF 215 ltlooommnaniiiocoeessss 8ootr 0BZ=-- =SE == == m == ==wz= i njoonnevniicosesss 32 B BZ= z ZT ZZT Z-= T Z == ===z& s3[oennseiiiccsse B Z A SS == == == == == 11Wo iiioobmnnnnii1000ss6sss 807 B== =Z 2 Z== ==ZT =zz=z === === " i1ia8 iiiooonnnaaaiiioo0ssesss 51110 0 == Z S S T Z= Z2=T -z = =z==z z== iiien iiiooonnnaaeiiioo0ssesss 11123 0= == SZ=z==ec zz === = = 521 iiioo0nnneneiiio0ossesss 2115 0== =SZT T=TT2SET=T E== z = B52ooiinoonnieeiieoosesss o i p=== Z Z2 T 2=Z== ====== : 335 lilooonmmaiiiccossssss 2511 = ==S 222 === == = = z= =z 35I iiooonnnreeiiicoosnesss s22m 3leneitE 5 == SE S s s z= =IS2 ==NS 3RS = = = ZS = = 2 = = 33Biniieoanneeiiigooesssss aoa Z2SSsS ==Z = z : z z= === : z 33imloeoimneoieoisss 334 = SSsZS 2=2 ==2 2==zz ==<2 z== B35 iinmcaeiioes:s 7 IIe | = SSE =Z = Z== === === =2z = Ss sSh=as :3 &a e e ies Rs 3RtNo b a SZS T z=2 == =z zz== === =: 000792 FACT: DATA REVIEW SUMMARY FORM Yel Date Review Sign oOewners Ze : NAesaeiyostRweviZew s acts satis Tose BoLlue, Tug 20 dieAldi= ccd Hy NToamks Riez wuss [by ose A, seup ui NS elo V Suan ag ils owt nAlC sleg an pe z Commas tats vo 22s (rine)= L 2alla,nt{ pUh issd.) bee e cd2s2i6p, I. etfda gla Ha Aeecbiacin black; A va dunloz. Sapien ieel f"oLm ibenionChocks: _J=> 1% -- Ear|pl l e hpoeee2sCongcre2X UC Ul Sr-- v murwlPeI-- vTA7. --rr E hoo 0.2 J---- yr ge L = ffilea akeoofa f L4da2 l--iFs=eHfH Olav ace pi AlbenionChecks: OK --> 17% psveso. Nobonglynd ith those songls 4 - Zs Wh, a [Dun Stame. 2K A 4 ee pose: ( _Prosr a aacsd Comenee1d0 (1): POAA wits hogplaualie iotae ipl; aiotded [roe foo toe hanse Lg _L-f300k 7 conc frazgpl et ant | evs. ple--bn Mocti lex _ogpossl [Ey = ChibestonChecks:_Jk-- 7/73s pore _Dabonobwed ose or nligls eoend Wc Bomanalysis lislas Tue lofey 7 000793 FACT: DATA REVIEW SUMMARY FORM one "ups . wxctnswarn Znsls voto ella,Duy22dedi =fWely eee tre -- Cwnnire Tehhy DataReviewSign-O Asser Newowzslrhe W[outepEi bopp\a63cul GSun TmvRen ows Dculus 2 5 = ciolmgsp oo mm crm oe pa BaconBlank M4 MaeBis espn: __ No6l dary) aml vaso y debe nob fran cope i Bg loc. - SE Ext [. fo sone used Ie Zblstl Sokpoe lowire LHdan dfudLoicokenrs de 4. bo Al Lb . a 5 7 ac lo pot 000794 meh pid hot foggh ane, debe nob wed. ne Wu lish 000795 iFH E Si iEmi ene m- s wsex BmoBBRR HEMav uclst 000796 bee tok So uniled ans on lesan ' Tren eke not ied BC lglg 000797 Bobo torgh Gralge. dda nok ued se lt 000798 --_-- Tel [CToohrmcaptoiuonnodcfuaveme:er:mP0iO05Rs4A:2Fu0n+9c80118058.6098 osvepypCea:arEx.tnOanmianSs:GE,xcAelruedea, Weighing: 1x,As ans: None + Jx I 1) 20 abo soho athe w%e0 keeCone 000799 (G(aCroanttoi enncot2ufrr amvrei:en:1a.tP1i0Fo1S1n:(xF.0u5n6c1?19)6425745 [[RCeasrpeoynspeetyCpon: tE,xtgomianl:SdE,xArcea, Weighting: 1x,Avstrans: None 1.36 | / | | oo ho acho abo ao 10600 1800cone 000500 ([(GGooCmuopcronuenindocfmuDarmover::Pm1RaO.SnA7x6(3F48u57n5.1c31)527587 [oRreespsoonseeyCp:eaE.xagrmial:SiEc.xAordeea, Waighing: ts,Aians No 25004 | || || I|" x / So a0 ashe eshs sso 16866 yes 200501 e-- e ---- retes-- etmeets [Response Faactmore, S48t75.5F4 un) (C[urvRetyepet:yspReF:pExtoemanlSus,Areea 5.420 x | | I 0 01 oz 03 os 05 08 07 08 09 10Gone. nOOBOZ get plw 000803 FE Li. SI ------ : _-- aT ri ema -- - Ce ~~ ooosoa _-- = -- 560505 . adn 2 - QuaIt, #r41oEE For hry Log 99`0S9O2UP 020199:PFAAGCET 1Eq1uipment. DATEL 111032 SATNUADLYYNSUTMiBsERZOXI45. MATRIX:alive DESCRIPTION; diluion-da2 FACT Method: EIS810 Injectionvolume:10a Cycleime 10,EleceodeZ:53my MobilePhase: (NOSMNHCHICG BMOH TNA-orsoddmabe (0) QUEL GINA Propacd (ey) (OLLI (BLUR Expires: wow mame Sowuroning? @ oe N Om Guardcoluma: None Poobersa Lotwmbens Anatacolumn:Keio Beasl SeeziShmn Tuell SeesmSm Par umber 955.012 LotmumberBELL Seal amberS802 Analyticalparameters: Dryinggs(UR)150_ ESTnebugalsUsmiLn3 g Shes asLAD LCFlowrte X00ulmi, Back pressor:G50 HPLCsolvent gradicat: 10min10:95 mTiean-- sim o 2100 e we se me 00 000806 BEST COPY AVAILABLE stag Method Report Page 1 sachods KASSLIX\0TPO20193. 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