Document E33Np7Lz1JQ54Y1ayDYDNj4b

AR22-OSOM ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, orFC-143. (Octanoic acid, pentadecafluoro, ammonium salt, CAS # 3825-26-1 ) Remarks: The 3M production lot number was 37. The test sample is FC143. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 -1 00 % test substance and 0 - 3.5% C6, C7, and C9 perfluoro analogue compounds. METHOD: Method: USEPA 660/3-75-009, 1975 Type: Static acute GLP: No Year completed: 1980 Species: Pimephalespromelas Supplier: Dale Fattig of Brady, Nebraska. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Probit analysis Test fish age: Not noted. Length and weight: Average length = 3.6 cm Average weight = 0.40 g Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry: Temperature: 17C Dissolved Oxygen: 10.0 ppm pH: 8.0 Test conditions remarks: A daily photoperiod of 16 hours light and 8 hours dard, with a 30 minute transition period was maintained throughout the acclimation and testing period. Stock and test solution preparation: Not noted Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: 35 X 20 X 25 cm deep glass aquariums containing 16 liters test solution. Number of replicates: two Number offish per replicate: 20 Loading rate: 0.50 g/L Number of concentrations: five plus a blank control Water chemistry during the study: Dissolved oxygen range (24 - 96 hours): 4.0 - 5.0 mg/L pH range (24 - 96 hours): 7.9 - 8.0 Test temperature (24 - 96 hours): 18-19C RESULTS Nominal concentrations: Bk control, 560, 650, 750, 870, and 1000 mg/L Element value: 96-hour LC50 = 766 (743 - 789) mg/L Element value based on nominal concentrations. CONCLUSIONS The test sample 96-hour LC50 for fathead minnow was determined to be 766 mg/L with a 95% Confidence Interval of 743 to 789 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing at the time it was conducted. However, sample purity was not properly characterized and it lacks analytical confirmation of test substance concentrations. REFERENCES 3M Technical Report Summary, Aquatic Toxicity Testing: FC-143 (Lot-37) L.R. 5625 S, Report Number 037, Project Number 9970012600, M. T. Elnabarawy, May 8, 1980. OTHER Last changed: 5/24/00 003781 Form 7 4 7 -1 1-A TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMUNICATIONS CENTER - 201-2CN Oat* __ ^5/8/8 0 'important - i f report sprinted on both sides o f paper, send two copies to TCC.) vision Environmental Laboratory (EE & PC), St. Paul, Minnesota Proiaet Commercial Chemicals Raport Titla ___ yS* ^ ) A Aquatic Toxicity Testing: / F C - 1 4 V (Lot-37) L.R. 5625 S To E. A. Reiner A uthor(s) _______ . M. T. Elnabarawy - 2-3E Notebook Rofaroneo 50012: 23 apt. Numbar 0535 Proiaet Numbar 9970012600 Raport Numbar 037 Em ployua N um barli) 46981 f t a of Fagoa Including Covarahaat 5_____________ SECURITY ^ 0pw1 Clotad (Spadai Authorization) 3M CH EM ICAL ^ REG ISTR Y W Naw Chamicala Raportad Yaa H No KEYWORDS: (Salact tarma from 3M Thataurut. Suggest othar applicala tarma.) CURRENT OBJECTIVE: To determine the acute aquatic LC5q following exposure to varying concentrations of FC-133 (lot-37). EE & PC - Div (Env. Lab) \xicity (Aquatic) REPORT ABSTRACT: (200-250 worda) Thia abstract inform ation ia diatributad by tha Technical Communicationa Cantar i alert 3M`era to Company R & D . The acute static aquatic 96-hour LC50 values obtained for fathead minnows (Pimephales promelas) following exposure to FC-143 in replicated experiments were 776(743-812) and 754 (723-787) mg/1. The combined 96hour LC50 was 766(743-789) mg/1. Data generated include LCen values with 95% confidence limits. ou Bacon J. Welter Inform atio Initiala: 003782 CONCLUSIONS In these replicated 96-hour LC50 aquatic tests, the calculated values for fathead minnows (Pimephales promelas) were 776 and 754 mg/1. Materials possessing an LC50 of this order of magnitude are considered to be practically nontoxic based on the NIOSII criteria for aquatic toxicity interpretation. (2 ) INTRODUCTION; The test substances is a white fine powder, soluble in water at room temperature. Tests performed on this material evaluated its potential aquatic toxicity and the data generated form the basis of this report. ACCLIMATION AND EXPERIMENTAL DESIGN: The test protocol utilized for this study was modeled after that described by USEPA (1975). (1) The fathead minnows (Pimephales promelas) were obtained from a private hatchery (a). Stock fish were held in a flow through system fiberglass holding tank filled with carbon-filtered well- water at 14-16 degrees C. A daily photoperiod of 16 hours light and 8 hours dark, with a 30-minute transition period was maintained throughout the acclimation and testing period. The fish were fed Tetra-Min (b) daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated to test temperature for 2 days prior to use in assays. Twenty (20) fathead minnows, uniform in size, were tested at each chemical. All-glass aquaria, 35x20x25 cm containing 16 liters of diluent plus toxicant comprised a study chamber. Test fish were randomly allocated to each test chamber within 30 minutes following toxicant addition. The fathead minnow experiments were conducted at 19+2 degrees C. Mortality, temperature, dissolved oxygen level, and pH of all test solutions were measured at 24-hour intervals or until total mortality had occurred. General observations were recorded relative to swimming pattern and behavioral changes induced by exposure to the test chemical. The acute short-term static bioassays were performed on FC-143 using carbon-filtered well water of known composition and constant quality as the diluent (Tables 1,2). The 96-hour fish studies were performed in replicate using the following FC-143 logarithmic concentrations; 560, 650, 750, 870, and 1 , 000 mg/1 . LC50 values with 95% confidence limits were calculated using the probit program and 3M TRAC Computer system. Monitoring equipment used included ASTM thermometers, Orion temperature-- compensating pll meter, a Yellow Springs dissolved oxygen meter, and a Taylor temperature chart recorder. a) Dale Fattig Fish Farm, Brady, Nebraska. b) Commercial Fish Food of known composition 0O37S3 RESULTS: The fathead minnows (Pimephales promelas) used in these studies had an average weight of 0.4 grams and a length of 3.6 cm. The loading factor was 0.5 g/1 ; the maximum recommended loading factor is 0.8 g/1 (1). Temperature, pH, and dissolved oxygen values and ranges obtained during this study were: 19+ 1 C., 7.9 - 8.0 pll units, and 96-hour dissolved oxygen values ranged from 4.0 - 5.0 mg/1. These latter values calculated out to a minimal oxygen saturation of 45-56%. In this replicated study, the fish 96-hour LC50 values were 776 and 754 mg/1. Chemicals possessing an LC50 value in this range are considered to be practically nontoxic to the aquatic environment (2 ). The data reported herein are based on studies performed by M.T. Elnabarawy. Accompanying data sheets comprise original data. A copy of this report is filed in the Environmental Laboratory Archive System, Building 2-3E. It should be noted that the generated results were reported in the absence of information relative to the "purity" and the "stability" of the chemical substance FC-143 (Lot 37). The reported LC50 values were calcuated on the basis of the "observed mortality rates" and in the absence of the actual measured final concentrations of PC-143 (Lot 37) in test solutions. REFERENCES: (1) US/EPA - Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians, Ecological Research Series 660/3-75-009, 1975. (2) Registry of Toxic Effects of Chemical Substances, '76 Edition, Christensen, H. E., and E. J. Fairchild, Eds., p. XV and Ci, U.S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, D.C. (June, 1976). G037S4 TABLE 1 WELL WATER QUALITY SAMPLE DATE: 4/30/80 SAMPLE TAKEN: 3M Bldg. 2-3E SAMPLE DATE 4/30/80 (TAKEN FROM TANK #4) PARAMETER pll Residual Chlorine Ammonia Nitrogen Conductivity Alkalinity Total Solids Total Hardness Total Fluoride Dilute COD Total Coliform Standard Plate Count Aluminum as Al* Arsenic as As* Boron as B* Cobalt as Co* Copper as Cu* Chromium as Cr* Cadmium as Cd* Iron as Fe* Nickel as Ni* Lead as Pb* Silver as Ag* Zinc as Zn* Mercury as Hg* CONCENTRATION 7.6 <0 . 0 2 mg/ 1 <0.1 mg/1 as N 400 umhos/cm at 25C 226 mg/ 1 as CaCO 378 mg/1 272 mg/1 as CaC03 <0 . 0 2 mg/ 1 1.3 mg/ 1 negative 42 per ml 0 . 0 1 mg/ 1 <0.005 mg/1 <0 . 2 mg/ 1 <0. 002 mg/ 1 0.018 mg/ 1 <0.005 mg/1 <0. 002 mg/ 1 0 .022 mg/ 1 0.005 mg/1 <0.005 mg/1 <0. 002 mg/ 1 0.13 mg/1 <0 . 2 ug/ 1 ENV. LAB WATER QUALIFY BOOK 8 9 !8 9 8 8 8 9 9 10 10 14 14 14 14 14 14 14 14 14 14 14 14 14 PCB's** <0.50 ug/1 36 Total Organochlorine** <0.50 ug/1 Pesticides 36 Total Organophosphorus** Pesticides <0.50 ug/1 36 * Sample Date 3/31/80 *`Sample Date 2/26/80 TABLE 2 ENVIRONMENTAL LABORATORY, BLDG. 2-3E, ST. PAUL, MINNESOTA FIRST QUARTERLY REPORT, 1980 WELL WATER SUPPLY FOR AQUATIC TOXICITY TESTING 1 PARAMETER January February March 24 26 31 AVERAGE (+ S) TOTAL HARDNESS* as mg/1 CaC03 260 268 264 264 + 4 TOTAL "M" ALKALINITY* as mg/1 CaCO 223 220 225 223 + 3 ELECTRICAL SPECIFIC CONDUCTANCE* as umhos per cm2 at 25C 380 370 400 383 + 15 TOTAL SOLIDS* as mg/1 350 408 402 387 + 32 pH** 7.6 7.6 7.6 7.6 + 0 STANDARD PLATE COUNT*** per 1.0 ml ) 29 30 19 26 + 6 CRITERIA OF CONSTANT QUALITY * Monthly ranges should be less than 10% of their respective averages. (Draf #10, ASTM Committee E-35 on Pesticides). ** Monthly range of pH should be less than 0.4 unit. (Draft #10, ASTM CommitteeE35 on Pesticides). Total bacterial densities should not be greater than 500 organisms per ml. (USEPA-600/8-78-017, page 101.) J