Document Dznk0m6YDD9B4w0vV7v8OZGd

p. 1 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-002 2/9/2010 12:18:12AM PFCkinetexLong.dam 22 Injection Volume Sample type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3021500 2357900 5613800 2837200 126120 5613800 161840 161840 161840 Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.38 (2.53) 3.34 (3.34) 4.14 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00 (5.14) 0.00(5.74) Analyte Area 0 0 28597 12002 5957 0 0 0 0 Calc. Cone (ng/mL) N/A N/A 0.062 0.069 0.028 N/A N/A N/A N/A Modified? No No No No No No No No No -'L~ ( f t * f ( Page 158 of 242 52 P-2 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT) 1.40(1.36) 1.40(1.36) 2.48(2.44) 2.32(2.44) 2.42 (2.53) 2.39 (2.53) 0.00(3.34) 3.35 (3.34) 0.00 (4.06) 4.15(4.06) 4.15(4.10) 4.79 (4.64) 4.78 (4.64) 5.32(5.13) 5.78 (5.56) Area 10864 3021500 464 2357900 8591 5613800 0 2837200 0 126120 730 1890 161840 486 298 Modified? Calc (Exp) MRM Ratio No 0.00 (2.38) Ratio Confirms ID No No 0.00(50.17) No No 0.00 (0.28) No No 0.00(19.17) No No 0.30(0.32) YES No 196.31 (22.53) No No 0.00 (0.15) No No 236.39 (22.10) No No 0.00 (0.22) No No 21.17(1.86) No No 0.00(0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00(0.36) No Page 159 of 242 53 P-3 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review K1000968-002 |- PFOA 1 413.0/169.0 Da o Baseline Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wronn Peak PFNA 1 463.0/419.0 Da o Baseline Missed Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Peak PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Pftak 4.14 r. 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Pnak J5lHKj 4.15 .Wjr) i PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Ppak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Ppak PFUnA 1 563.0/519.0 Da Baseline Missed ___i Wrnnn Peak PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Pnak TJ/: II!:. Hi 5(1 13C-PFUnA 565.0/270.0 Da o Baseline Missed n Wrnnn Peak I 40 -5 5(I 55 13C-PFDoA 615.0/169.0 Da Baseline Missed n Wrnnn P eak ?! i i a" PFDS 1 599.0/80.0 Da o Baseline Missed p Wrong PoaK 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak * | 45 50 - 55 PFTrA 1 663.0/619.0 Da Baseline Missed n Wronn Peak_____ < ~ i4 13C-PFDoA 615.0/169.0 Da D Baseline a Miss h Wrnnn Peak PFTeA 1 713.0/669.0 Da Baseline Missed ___11 Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da u Baseline Missed ____n Wrnnn Peak___ Before After Page 160 of 242 54 Createci with Analyit Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation :' j.fi.ii1. ! ...... 1.40 - PFOA2 413.0/369.0 Da Baseline Missed c Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak K1000968-002 .. ?;[ ___ 'jlc 4 :o : 5 u PFOS2 499.0/99.0 Da Baseline o Missed Wrong Peak 1| t j" jijcj fl I| PFDA2 513.0/269.0 Da Baseline Missed c Wrong Peak PFUnA2 563.0/269.0 Da Baseline g Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed c Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed d Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 161 of 242 55 j r f C o lu m b ia Analytical S o n /ioe r P- 5 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-002 2/9/2010 11:04:34 AM PFCkinetexShort.dam 22 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 3872900 4872600 3232200 4872600 4872600 3232200 5160100 5160100 Analyte RT (Exp RT ) 1.27 (1.27) 3.59 (3.60) 4.25 (4.26) 5.31 (5.31) 6.23 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65) Analyte Area 38448 32836 6328 65076 15514 47194 3232200 5160100 Calc. Cone (ng/mL) 0.04 0.05 0.02 0.07 0.02 ^f 0.04 0.71 1.00 Modified? No No No No No No No No '((0 Page 141 of 245 56 p. 6 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T) 1.27 (1.27) 3.59 (3.60) 0.00 (4.26) 0.00 (5.31) 6.21 (6.22) 6.31 (6.32) 1.26(1.26) 5.30 (5.30) Area 38448 32836 0 0 75480 16644 3872900 4872600 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.00(0.40) No No 0.00(0.10) No 4.87(3.03) N / No J / No 0.35 (0.40) YES No 100.73(13.55) No No 74.88(13.06) No Page 142 of 245 57 P-7 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review I PFBA 1 213.0/169.0 Da Baseline Missed n Wronn Peak 4.25 PFBS 1 299.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wronn Peak 6.3 ,L_ 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak K1000968-002 3.59 ...A PFPeA 1 263.0/219.0 Da Baseline a Missed n Wrnnn Poak 13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Peak 13C-PFHXA 315.0/270.0 Da u Baseline Missed n Wrnnn Peak PFHpA 1 363.0/169.0 Da Baseline o Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da d Baseline Missed n Wrnnn Peak S_180-PFHS 403.0/103.0 Da o Baseline Missed ____n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline d Missed ___n Wrnnn Peak___ S_13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___ Page 143 of 245 58 p. 8 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation . = f : ..... f ............. | .... 3.59 ..j f c - ------~ PFBA 2 213.0/169.0 Da 0 Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak K1000968-002 f 1 i'""' i`j "AT <!< J`. Vl PFBS2 299.0/99.0 Da Baseline Missed n Wrong Peak `.00c .uuu t- J0j .'two iuw J) 1 PFHxA 2 313.0/119.0 Da DBaseline c Missed Wrong Peak Ti 'H f .. 6f aA A. S "T i im -Kiiui 00 .. .. s. n............. :v* Lu:-- 00 o'. -ti ............... r....... i `,G.S l\ PFHpA 2 363.0/319.0 Da c Baseline o Missed a Wrong Peak PFHxS 2 399.0/99.0 Da Baseline Missed 0 Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After Thisinageisrtolavaiable N/A Baseline Missed Wrong Peak Page 144 of 245 59 p.9 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-003 2/9/2010 12:25:44 AM PFCkinetexLong.dam 23 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3169100 2469400 5529200 2691200 93048 5529200 134090 134090 134090 Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.37 (2.53) 0.00(3.34) 4.14 (4.06) 4.15 (4.10) 4.80 (4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 0 0 21861 0 4293 10027 4664 0 0 Calc. Cone (ng/mL) N/A N/A 0.052 N/A 0.027 0.036 0.030 N/A N/A Modified? No No No No No No No No No Page 162 of 242 60 mm Created with A nalyif Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.37(1.36) 1.37 (1.36) 2.27 (2.44) 2.36 (2.44) 2.39 (2.53) 2.38 (2.53) 0.00 (3.34) 3.35 (3.34) 0.00 (4.06) 4.15(4.06) 4.15 (4.10) 4.83 (4.64) 4.78 (4.64) 5.32 (5.13) 5.77(5.56) Area 6004 3169100 330 2469400 4849 5529200 0 2691200 0 93048 2719 1154 134090 937 765 Modified? Calc (Exp) MRM Ratio No 0.00 (2.38) Ratio Confirms ID No No 0.00 (50.17) No No 0.00 (0.28) No No 0.00 (19.17) No No 0.22 (0.32) No No 252.93 (22.53) No No 0.00 (0.15) No No 0.00(22.10) No No 0.00 (0.22) No No 21.67(1.86) No No 0.27(0.32) YES No 0.25(0.29) YES No 28.75 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No Page 163 of 242 61 p. 11 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review K1000968-003 PFOA 1 413.0/169.0 Da p Baseline Missed ___r. Wronn P eak__ ( 2.37 i..1 PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak 4.14 13C-PFOA 417.0/372.0 Da Baseline a Missed ___n Wronn Peak 2.38 1 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak 4.15 Ifl'lifJ f " f,, .//>'-/ ... PJVvA'yi/'-M'**'''-'o'-'\ PFNA 1 463.0/419.0 Da Baseline Missed ____n Wronn Peak___ 11500 ! i !w !\ 13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Peak 4*5 8" | 1-5 PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak f- !v, 411 45 `C 11 401 PFUnA 1 563.0/519.0 Da c Baseline Missed r, Wrnnn Peak 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak PFDS 1 599.0/80.0 Da a Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da c Baseline u Missed Wrnnn Peak 46 45 PFDoA 1 613.0/569.0 Da c Baseline Missed n Wrnnn Peak IH 13C-PFDOA 615.0/169.0 Da Baseline Missed Wrnnn Peak 5G 55 GO PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed ____n Wrnnn Peak___ - n ,a .! , m| '-M'"1\.'i ''a1*!' V I PFTeA 1 713.0/669.0 Da d Baseline o Missed n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed ___n W rong Peak___ Before After Page 164 of 242 62 p. 12 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation ! Tl\ ' 1.37 "*j: --- Jkkr- I ..| PFOA2 413.0/369.0 Da Baseline o Missed o Wrong Peak PFNA2 463.0/219.0 Da o Baseline Missed Wrong Peak K1000968-003 ? -i A PFOS 2 499.0/99.0 Da d Baseline Missed d Wrong Peak 1w i * PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da o Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 165 of 242 63 p. 13 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-003 2/9/2010 11:14:07 AM PFCkinetexShort.dam 23 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 765720 5793500 3367300 5793500 5793500 3367300 5105600 5105600 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.29 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65) Analyte Area 13453 26516 8911 45833 16118 48962 3367300 5105600 Calc. Cone (ng/mL) 0.11 0.03 0.03 0.02 0.01 0.04 0.75 1.00 Modified? No No No No No No No No Page 145 of 245 64 p. 14 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT) 1.26 (1.27) 3.59(3.60) 4.24 (4.26) 0.00 (5.31) 6.21 (6.22) 6.30(6.32) 1.26(1.26) 5.30(5.30) Area 13453 26516 3303 0 126570 22573 765720 5793500 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1,00) YES No 0.37(0.40) YES No 0.00(0.10) No No 7.85 (3.03) No No 0.46(0.40) YES No 56.92(13.55) No No 126.40(13.06) No Page 146 of 245 65 fumbia Quantitative Peak Review p. 15 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-003 Before After Page 147 of 245 66 p. 16 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation K1000968-003 " j 1 26 J !- i i --11 3.59 :: *" k ... .... .. PFBA 2 213.0/169.0 Da c Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da d Baseline c Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline o Missed Wrong Peak ,,I 6.21 ;d i . .r a . PFHpA2 363.0/319.0 Da Baseline Missed o Wrong Peak J1 * JCIV .... 6.$Q _.......... i; Id 5', 60Timmanf>S ?! PFHxS 2 399.0/99.0 Da D Baseline Missed Wrong Peak l?*t] 1 i * 5*'l ...' S 13C-PFDA 515.0/470.0 Da q Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA2 313.0/119.0 Da Baseline Missed Wrong Peak Tinsiiiageisrwtayaiable N/A Baseline Missed Wrong Peak Page 148 of 245 67 p. 17 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\Samplesl_ong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-004 2/9/2010 12:33:18 AM PFCkinetexLong.dam 24 injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFD0A IS Area 2746100 2237700 5254600 2170500 97278 5254600 137900 137900 137900 Analyte RT (Exp RT ) 1.37 (1.36) 0.00 (2.42) 2.38 (2.53) 0.00 (3.34) 4.13 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00 (5.14) 0.00(5.74) Analyte Area 134500 Calc. Cone (ng/mL) 1.040 0 N/A 97866 0.189 0 N/A ftf fh 0 N/A 0 N/A 0 N/A 0 N/A Modified? No No No No No No No No No ( Page 166 of 242 68 Created with Analyst* Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary | Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 SJ3C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.37 (1.36) 1.38(1.36) 2.34 (2.44) 2.35(2.44) 2.40 (2.53) 2.38 (2.53) 0.00 (3.34) 3.35(3.34) 0.00 (4.06) 4.15 (4.06) 4.14(4.10) 4.80 (4.64) 4.78 (4.64) 5.30(5.13) 5.48 (5.56) Area 263240 2746100 543 2237700 29686 5254600 0 2170500 0 97278 718 762 137900 370 149 Modified? No Calc (Exp) MRM Ratio 1.96 (2.38) Ratio Confirms ID YES No 20.42 (50.17) No No 0.00(0.28) No No 0.00(19.17) No No 0.30 (0.32) YES No 53.69 (22.53) No No 0.00(0.15) No No 0.00 (22.10) No No 0.00 (0.22) No No 20.04 (1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No Page 167 of 242 69 Columbia K p. 19 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review LL1.37 s ' i :: K1000968-004 PFOA 1 413.0/169.0 Da c Baseline Missed n Wronn Peak l.L PFOS 1 499.0/80.0 Da Baseline Missed r, Wrnnn Peak 4.13 ?i 13C-PFOA 417.0/372.0 Da Baseline Missed ___n Wronn Peak___ 13C-PFOS 503.0/80.0 Da d Baseline o Missed n Wrnnn Peak 4.15 PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Ppak 13C-PFNA 468.0/423.0 Da Baseline Missed n Wronn Peak PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak Sw PFUnA 1 563.0/519.0 Da a Baseline Missed n Wrnnn Peak '"..A-; -----Jo------ oT------ -----PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak .,uo r PFTeA 1 713.0/669.0 Da o Baseline c Missed n Wrnnn Peak___ 13C-PFUnA PFDS 1 565.0/270.0 Da 599.0/80.0 Da d Baseline Missed Baseline Missed ___n Wrnnn Peak___ n Wrnnn Peak___ _ l_4.78 K>H> 80'; 7 bfiU -0 1 onV W '/ W S . - " ''V W V il i) 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak 54 194 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak___ 130-PFDoA 615.0/169.0 Da d Baseline a Missed n Wrnnn Peak___ JL 13C-PFDoA 615.0/169.0 Da Baseline Missed n Wrnnn Peak___ Before After Page 168 of 242 70 Created with A n a l^ R e p o rte r Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation 1.37 ............... I ............. ::j 1 . "-I 1 ;r.ir. j. fl<) fi PFOA 2 413.0/369.0 Da Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed d Wrong Peak M li . 1 koyj 11 M y" i ;Gon 4.14iC'flil I 5- in JS iO PFUnA 2 563.0/269.0 Da Baseline Missed Wrong Peak PFDS"2 599.0/99.0 Da Baseline Missed Wrong Peak "w` 1- UMl i ) K1000968-004 .5 ,,,,,, 1 r<j|n k PFOS'2 499.0/99.0 Da Baseline Missed Wrong Peak lEltO c P0M s in 5 <0 PFDA 2 513.0/269.0 Da Baseline Missed Wrong Peak iflue m & KX.i - \ -*M 4l| i\, PFD0 A 2 613.0/169.0 Da Baseline 0 Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Misse Wrong Peak /- PFTeA2 713.0/169.0 Da Baseline c Missed Wrong Peak Before After Page 169 of 242 71 Iiim bia Created with A n a ls t importer Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-004 2/9/2010 11:23:40 AM PFCkinetexShort.dam 24 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 3903100 4515200 3062600 4515200 4515200 3062600 4189100 4189100 Analyte RT (Exp RT ) 1.25 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 58649 84885 57657 131460 26357 360350 3062600 4189100 Calc. Cone (ng/mL) 0.08 0.18 0.07 0.22 0.09 0.35 0.83 1.00 Modified? No No No No No No No No U Page 149 of 245 72 iumfoia p. 22 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.25(1.27) 3.59(3.60) 4.26 (4.26) 5.29(5.31) 6.22 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30) Area 58649 84885 22150 13026 135750 140170 3903100 4515200 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.38(0.40) YES No 0.10(0.10) YES No 5.15(3.03) No a M x , No 0.39(0.40) YES No 66.55(13.55) No No 34.35(13.06) No Page 150 of 245 73 Quantitative Peak Review Created with Ana Iysi Kteporter Printed: 15/02/2010 2:34:00 PM K1000968-004 Before After Page 151 of 245 74 Columbia Aualvtical Strvicos" p. 24 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation ! 1.25 K1000968-004 1 3.59 . . - - - ..........1 ...... ........ 1 ____ PFBA 2 213.0/169.0 Da Baseline Missed Wrong Peak io*<;d ". | bl) .'I -` T " 1 PFPeA2 263.0/219.0 Da Baseline Missed d Wrong Peak PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak *4 65 1 6.81 a E 65 ............ i ............. 1 :,V5 ll PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Tteimagebnoisvaiable PFHpA 2 363.0/319.0 Da c Baseline o Missed c Wrong Peak PFHxS 2 399.0/99.0 Da Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed q Wrong Peak N/A q Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After Page 152 of 245 75 S ervicer Created with Anafys?&eporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\Samplesl_ong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-005 2/9/2010 12:40:50 AM PFCkinetexLong.dam 25 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 2868800 2129300 5081100 2575600 97797 5081100 132660 132660 132660 Analyte RT (Exp RT ) 1.38 (1.36) 0.00 (2.42) 2.38 (2.53) 0.00(3.34) 4.13(4.06) 4.13 (4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 134790 0 87278 0 4589 2323 0 0 0 Calc. Cone (ng/mL) 1.000 N/A 0.176 N/A 0.028 0.024 N/A N/A N/A Modified? No No No No No No No No No ^ f f k l (C Page 170 of 242 76 Created with Analyfi:' ^Iporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S J3C -P F O S PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp R T ) 1.38 (1.36) 1.40(1.36) 2.31 (2.44) 2.34 (2.44) 2.39 (2.53) 2.38 (2.53) 0.00 (3.34) 3.34 (3.34) 0.00 (4.06) 4.14(4.06) 4.16 (4.10) 4.80 (4.64) 4.77(4.64) 5.28 (5.13) 5.43 (5.56) Area 263600 2868800 1208 2129300 27077 5081100 0 2575600 0 97797 802 1171 132660 532 167 Modified? Calc (Exp) MRM Ratio No 1.96(2.38) Ratio Confirms ID YES No 21.28 (50.17) No No 0.00 (0.28) No No 0.00(19.17) No No 0.31 (0.32) YES No 58.22 (22.53) No No 0.00 (0.15) No No 0.00 (22.10) No No 0.00(0.22) No No 21.31 (1.86) No No 0.35(0.32) YES No 0.00 (0.29) No No 0.00(3.08) No No 0.00(0.32) No No 0.00(0.36) No Page 171 of 242 77 Services' Quantitative Peak Review p. 27 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000968-005 PFOA 1 413.0/169.0 Da c Baseline Missed Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wrnnn Peak j ' PFNA 1 463.0/419.0 Da Baseline Missed Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed Wrnnn Peak PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Pnak 4.13 13C-PFOS 503.0/80.0 Da Baseline Missed Wrnnn Ppak PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Pnak 13C-PFDA 515.0/470.0 Da d Baseline Missed Wrnnn Pnak PFUnA 1 563.0/519.0 Da a Baseline c Missed ___n Wrnnn Peak 13C-PFUnA 565.0/270.0 Da d Baseline Missed ___11 Wrnnn Peak___ | . |s f PFDS 1 599.0/80.0 Da Baseline Missed Wrnnn Peak 13C-PFOS 503.0/80.0 Da d Baseline Missed Wrnnn Peak PFDoA 1 613.0/569.0 Da li Baseline Missed n Wrnnn Peak % A 63 t5 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Ppak Ib f1 -T: 1-J.J Stoil PFTrA 1 663.0/619.0 Da c Baseline Missed n Wrnnn Pnak___ PFTeA 1 713.0/669.0 Da Baseline Missed ___r Wrnnn Peak_____ 13C-PFDOA 615.0/169.0 Da G Baseline o Missed n Wrnnn Peak_____ Before After 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak Page 172 of 242 78 Services' p. 28 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation 1.38 ok. PFOA2 413.0/369.0 Da Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed d Wrong Peak bOU |* `(X* ,m MK1000968-005 >Uw PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline a Misse Wrong Peak PFUnA2 563.0/269.0 Da o Baseline Missed u Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed o Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed d Wrong Peak PFTeA2 713.0/169.0 Da o Baseline Missed c Wrong Peak Before After Page 173 of 242 79 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-005 2/9/2010 11:33:12 AM PFCkinetexShort.dam 25 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 13C-PFHxA PFHxS1 180-PFHS S_180-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 3952400 4853600 3167200 4853600 4853600 3167200 4413900 4413900 Analyte RT (Exp RT ) 1.26 (1.27) 3.58 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.31 (6.32) 6,31 (6.31) 7.64 (7.65) Analyte Area 57679 85108 58726 131600 31533 393280 3167200 4413900 Calc. Cone (ng/mL) 0.08 0.16 0.07 0.20 0.11 0.36 0.81 1.00 Modified? No No No No No No No No K i ^ '0 Page 153 of 245 80 p. 30 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.26 (1.27) 3.58(3.60) 4.24 (4.26) 5.30(5.31) 6.21 (6.22) 6.31 (6.32) 1.27 (1.26) 5.30(5.30) Area 57679 85108 22273 12220 90283 143270 3952400 4853600 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms 10 YES No 1.00(1.00) YES No 0.38(0.40) YES No 0.09(0.10) YES No 2.86(3.03) YES No 0.36(0.40) YES No 68.52 (13.55) No No 36.88(13.06) No Page 154 of 245 81 Created with Anafys?keporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review 1.26 1 PFBA 1 213.0/169.0 Da Baseline Missed r Wrnnn Pftak i 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak K1000968-005 3.58 PFPeA 1 263.0/219.0 Da Baseline Missed n Wrnnn Paak 5.30 13C-PFHxA 315.0/270.0 Da a Baseline Missed n Wrnnn Peak PFBS 1 299.0/80.0 Da Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Pfiak PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Pnak 13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Pftak PFHpA 1 363.0/169.0 Da u Baseline u Missed n Wrnnn Pftak 6.31 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da Baseline o Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline o Missed n Wrnnn Peak S_180-PFHS 403.0/103.0 Da a Baseline a Missed ___ n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da Baseline Missed ___n W rnnn Peak___ SJ3C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Poak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___ Page 155 of 245 82 Services" Created with Analyfi- Importer Printed: 15/02/2010 2:34:00 PM Ion Ratio :i 1.i^6 -Confirmation .... 3.58. 10 !$t 4fl <5 PFBA2 213.0/169.0 Da Baseline Missed c Wrong Peak PFPeA 2 263.0/219.0 Da Baseline Missed Wrong Peak 6.^1 ,0.5 K1000968-005 PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline a Missed Wrong Peak 6: 1 ,'iw _______A _________ PFHpA2 363.0/319.0 Da Baseline o Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 156 of 245 83 S trvi r p. 33 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-006 2/9/2010 12:48:24 AM PFCkinetexLong.dam 26 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3058100 2656400 6491000 2634200 142770 6491000 190670 190670 190670 Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.41 (2.53) 0.00 (3.34) 4.13 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74) Analyte Area 0 0 54779 0 6909 0 0 0 0 Calc. Cone (ng/mL) N/A N/A 0.094 N/A 0.029 N/A N/A N/A N/A Modified? No No No No No No No No No f Of Page 174 of 242 84 Created with Analyst- Rporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 SJ3C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.40(1.36) 1.36(1.36) 2.60 (2.44) 2.34 (2.44) 2.39(2.53) 2.35 (2.53) 0.00 (3.34) 3.31 (3.34) 0.00 (4.06) 4.13(4.06) 4.12(4.10) 4.78 (4.64) 4.76 (4.64) 5.27(5.13) 5.73(5.56) Area 14938 3058100 215 2656400 15893 6491000 0 2634200 0 142770 593 799 190670 332 154 Modified? Calc (Exp) MRM Ratio No 0.00 (2.38) Ratio Confirms ID No No 0.00 (50.17) No No 0.00 (0.28) No No 0.00(19.17) No No 0.29 (0.32) YES No 118.49 (22.53) No No 0.00 (0.15) No No 0.00 (22.10) No No 0.00(0.22) No No 20.66(1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00(3.08) No No 0.00 (0.32) No No 0.00 (0.36) No Page 175 of 242 85 y? o v J i Analytical San/icas" Created with Anafys?$?eporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review K1000968-006 ICJ\ /" >, DIM) $ :-5*s 20*5 s 2.34 i :-(iu ... Iflrt j. it U * / i ::: 1 "* 1 j.-.r V ,.*4 /Vv'7 V ;'ii ns in 15 a 15 20 ?5 JiV i5 DO H; PFOA 1 413.0/169.0 Da Baseline o Missed n Wrnnn Peak - tfrtl 1 -flOU 1IAV 13C-PFOA PFNA1 13C-PFNA 417.0/372.0 Da 463.0/419.0 Da 468.0/423.0 Da Baseline Missed Baseline Missed Baseline o Missed _______n Wrnnn Peak_____ n Wrnnn Peak 5s 2.35 41301 ir *' I :: '4 L DO i'5 30 - iI 200 jl , W hi n _______n Wrnnn Peak_____ 4*5 ? f, , ;;; v \a I 40 25 3D 35 40 PFOS 1 1 3C-PFOS 499.0/80.0 Da 503.0/80.0 Da u Baseline Missed Baseline o Missed n Wrnnn Peak 4 .J3 In Wrnnn Peak i 50*3 4J J*. SO PFDA 1 513.0/469.0 Da QBaseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed _____ n Wrnnn Peak_____ 5*5 2.35 , ... i: 500 i i; 1*5 35 J5 45 50 -'l 30 PFUnA 1 13C-PFUnA 563.0/519.0 Da 565.0/270.0 Da Baseline Missed Baseline Missed _______n Wronn Peak_____ _______n Wrnnn Peak_____ II.I.'MOV.ti 4* 4.^6 4*4 i JW1 jrt * i 1*4 ni | 40 45 4U c.s 40 45 50 45 PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak iji'.i 13C-PFDOA 615.0/169.0 Da OBaseline Missed n Wrnnn Peak 5*4 4*4 l* PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 1800 COD 4tfl 200 i 45 5T0immin 55 PFTrA 1 60 663.0/619.0 Da Baseline Missed n Wrnnn Peak_____ 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak I4*4 40 45 5<l 56 13C-PFDOA 615.0/169.0 Da Baseline a Missed n Wrnnn Peak !: i- stl 65 f.U PFTeA 1 713.0/669.0 Da DBaseline Missed n Wrnnn Peak_____ 40 45 i Si 45 13C-PFDoA 615.0/169.0 Da Baseline Missed n Wrnnn Peak_____ Before After Page 176 of 242 86 p. 36 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation K1000968-006 1.40 PFOA2 413.0/369.0 Da c Baseline a Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da o Baseline Missed a Wrong Peak PFDA2 513.0/269.0 Da c Baseline Missed Wrong Peak PFUnA2 563.0/269.0 Da u Baseline Missed Wrong Peak PFDS 2 599.0/99.0 Da Baseline Missed c Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da c Baseline Missed Wrong Peak Before D After Page 177 of 242 87 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-006 2/9/2010 11:42:45 AM PFCkinetexShort.dam 26 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 4026700 4918900 3265500 4918900 4918900 3265500 4342000 4342000 Analyte RT (Exp RT ) 1.25 (1.27) 3.60 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65) Analyte Area 48743 41065 14247 64104 17041 129210 3265500 4342000 Calc. Cone (ng/mL) 0.06 0.07 0.03 0.07 0.03 0.11 0.85 1.00 Modified? No No No No No No No No / 10 Page 157 of 245 88 Createci with Analyil-f?porter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.25(1.27) 3.60(3.60) 4.25(4.26) 0.00(5.31) 6.22 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30) Area 48743 41065 5621 0 196150 47255 4026700 4918900 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.39(0.40) YES No 0.00 (0.10) No 11.51 (3.03) No / M i No \ j No 0.37(0.40) YES No 82.61 (13.55) No No 76.73 (13.06) No Page 158 of 245 89 / T Columbia O Analytical Quantitative Peak Review p. 39 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-006 PFBA 1 213.0/169.0 Da c Baseline Missed n Wronn Peak 4.25 13C-PFBA 217.0/172.0 Da g Baseline o Missed n Wronn Peak PFPeA 1 263.0/219.0 Da o Baseline Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wronn Peak PFBS 1 299.0/80.0 Da o Baseline Missed n Wronn Peak 180-PFHS 403.0/103.0 Da Baseline Missed n W ronn Peak PFFIxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Ppak 13C-PFHXA 315.0/270.0 Da u Baseline Missed i Wrnnn Peak PFHpA 1 363.0/169.0 Da u Baseline o Missed n Wronn Peak 13C-PFHXA 315.0/270.0 Da Baseline a Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da a Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da d Baseline o Missed n Wrnnn Ppak S_180-PFHS 403.0/103.0 Da Baseline c Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline a Missed ___n Wrnnn Peak___ S_13C-PFDA 515.0/470.0 Da o Baseline o Missed n Wrnnn Peak Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ___n Wrnnn Peak___ Page 159 of 245 90 Created with AnalystReporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation ..i - 1.|5 9i k, 50> 3.60 PFBA 2 213.0/169.0 Da Baseline Missed q Wrong Peak PFPeA2 263.0/219.0 Da Baseline d Missed Wrong Peak K1000968-006 ,,, I lijilrt | ;ij PFBS2 299.0/99.0 Da c Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline c Misse Wrong Peak e is nol miabte PFHpA2 363.0/319.0 Da o Baseline o Missed Wrong Peak PFHxS2 399.0/99.0 Da o Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 160 of 245 91 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-007 2/9/2010 12:55:57 AM PFCkinetexLong.dam 27 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 2873000 2168100 5775700 2338800 105820 5775700 137230 137230 137230 Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.34 (2.53) 0.00 (3.34) 4.11 (4.06) 0.00(4.10) 0.00 (4.63) 0.00 (5.14) 0.00(5.74) Analyte Area 0 0 37941 0 3158 0 0 0 0 Calc. Cone (ng/mL) N/A N/A 0.076 N/A 0.010 N/A N/A N/A N/A Modified? No No No No No No No No No A t',. \y < '*7 i` id Page 178 of 242 92 Services' p. 42 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S J3C -P F O S PF&A2 S_13C PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDOA PFTrA 2 PFTeA2 L\ RT (Exp RT ) 1.34(1.36) 1.38(1.36) 2.32(2.44) 2.32 (2.44) 2.37 (2.53) 2.36 (2.53) 0.00 (3.34) 3.32(3.34) 0.00(4.06) 4.12(4.06) 4.11 (4.10) 4.80 (4.64) 4.75(4.64) 5.09 (5.13) 5.74 (5.56) Area 10650 2873000 239 2168100 11972 5775700 0 2338800 0 105820 458 692 137230 465 66 Modified? Calc (Exp) MRM Ratio No 0.00 (2.38) Ratio Confirms ID No No 0.00(50.17) No No 0.00(0.28) No No 0.00(19.17) No No 0.32(0.32) YES No 152.23 (22.53) No No 0.00 (0.15) No No 0.00 (22.10) No No 0.00(0.22) No No 33.50(1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No Page 179 of 242 93 Created with Anal^sti^eporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review K1000968-007 I PFOA 1 413.0/169.0 Da Baseline a Missed n Wrnnn Peak 2.34 i k PFOS 1 499.0/80.0 Da c Baseline Missed n Wrnnn Pnak 1! \j v* - PFUnA 1 563.0/519.0 Da c Baseline Missed ___n Wrnnn Peak___ 13C-PFOA 417.0/372.0 Da Baseline a Missed n Wrnnn Peak 2.36 I 13C-PFOS 503.0/80.0 Da Baseline a Missed n Wrnnn Ppak 4.12 PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak a I | f i, .v M v 'v V v V a i5 3n PFDA1 513.0/469.0 Da o Baseline Missed n Wrnnn Ppak 13C-PFNA 468.0/423.0 Da d Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Ppak f :: 45 13C-PFUnA 565.0/270.0 Da Baseline Missed ___n Wrnnn Peak 50 PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak ^V 7? 90 SS PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da o Baseline Missed n Wrnnn Pnak '< 1 (lj\ i',i i' . . Illl A.I l\ \ \ PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak___ I .Ij h A ;v \. PFTeA 1 713.0/669.0 Da Baseline Missed ___n W rnnn Peak___ 13C-PFDoA 615.0/169.0 Da Baseline Missed ___n W rnnn Peak___ Before After Page 180 of 242 94 Ion Ratio Confirmation p. 44 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000968-007 Before After Page 181 of 242 95 S e rv ic e s " p. 45 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-007 2/9/2010 11:52:17 AM PFCkinetexShort.dam 27 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 3969700 4943900 3357100 4943900 4943900 3357100 5041100 5041100 Analyte RT (Exp RT ) 1.25 (1.27) 3.59 (3.60) 4.24 (4.26) 5.29 (5.31) 6.23 (6.23) 6.31 (6.32) 6.30 (6.31) 7.64 (7.65) Analyte Area 36755 26848 13029 63283 14858 132780 3357100 5041100 Calc. Cone (ng/mL) 0.03 0.04 0.03 0.07 0.02 i4 * ' 0.11 0.76 1.00 Modified? No No No No No No No No Page 161 of 245 96 p. 46 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.25(1.27) 3.59(3.60) 4.25(4.26) 5.30(5.31) 6.21 (6.22) 6.31 (6.32) 1.26(1.26) 5.30 (5.30) Area 36755 26848 5369 3265 138840 47901 3969700 4943900 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.41 (0.40) YES No 0.05(0.10) YES No 9.34(3.03) No No 0.36 (0.40) YES No 108.00(13.55) No No 78.12(13.06) No Page 162 of 245 97 p. 47 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review 1 I1.25 PFBA 1 213.0/169.0 Da o Baseline Missed n Wronn Peak 13C-PFBA 217.0/172.0 Da c Baseline Missed n Wronn Peak K1000968-007 44 fa. 3.59 _____ __ Jk-.....----- - - 4(1*6 2u*S PFPeA 1 263.0/219.0 Da Baseline Missed n Wronn Peak 13C-PFHxA 315.0/270.0 Da Baseline a Missed n Wronn Peak .... j PFBS 1 299.0/80.0 Da c Baseline Missed n Wronn Peak 180-PFHS 403.0/103.0 Da o Baseline Missed n Wrnnn Paak 5.30 PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Paak 4*4 6.31 13C-PFHxA 315.0/270.0 Da d Baseline Missed n Wrnnn Peak 1 1 ....................... PFHpA 1 363.0/169.0 Da Baseline Missed n Wrnnn Paak 6.: 1 Tin. mm 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak l TuT.fr min PFHxS 1 399.0/80.0 Da Baseline o Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Ppak S_180-PFHS 403.0/103.0 Da Baseline d Missed n Wrnnn Pftak___ 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed ___ r, Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Pfiak Page 163 of 245 98 tf Ion Ratio Confirmation p. 48 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-007 3.59 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da d Baseline o Missed Wrong Peak i % 5 1 '* ! ................. 6 . ( 1 ......................... t iM 50e5 PFHpA2 363.0/319.0 Da g Baseline Missed Wrong Peak 55 h0 fiS ;o PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After (30 Jk- PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Ife mageisnoi valable N/A Baseline Missed Wrong Peak Page 164 of 245 99 Created with Analfst^eporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810b\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-008 2/9/2010 1:03:33 AM PFCkinetexLong.dam 28 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3102200 2290200 5389800 2593000 95798 5389800 128910 128910 128910 Analyte RT (Exp RT ) 1.39 (1.36) 2.35 (2.42) 2.35 (2.53) 3.31 (3.34) 4.10 (4.06) 4.11 (4.10) 4.76 (4.63) 5.27 (5.14) 0.00(5.74) Analyte Area 242140 28559 1453100 24564 14457 19506 6892 3821 0 Calc. Cone (ng/mL) 1.640 0.133 2.540 0.119 0.138 0.051 0.064 0.047 N/A Modified? No No No No No No No No No \V 'V z^ "c 100 Page 182 of 242 p. 50 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary 1 Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT) 1.38(1.36) 1.40(1.36) 2.34 (2.44) 2.31 (2.44) 2.37(2.53) 2.35 (2.53) 3.31 (3.34) 3.31 (3.34) 0.00 (4.06) 4.11 (4.06) 4.10 (4.10) 4.73 (4.64) 4.74 (4.64) 5.26(5.13) 5.73(5.56) Area 508600 3102200 8510 2290200 481410 5389800 2887 2593000 0 95798 6637 2900 128910 1210 1303 Modified? Calc (Exp) MRM Ratio No 2.10(2.38) Ratio Confirms ID YES No 12.81 (50.17) No No 0.30(0.28) YES No 80.19(19.17) No No 0.33(0.32) YES No 3.71 (22.53) No No 0.12(0.15) YES No 105.56(22.10) No No 0.00 (0.22) No No 6.63(1.86) No No 0.34(0.32) YES No 0.42 (0.29) No No 18.70 (3.08) No No 0.32(0.32) YES No 0.00 (0.36) No Page 183 of 242 101 Colum bia Analytical Services' Quantitative Peak Review 1.40 p. 51 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000968-008 PFOA 1 413.0/169.0 Da Baseline Missed n \A/rnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wrnnn Peak___ PFNA 1 463.0/419.0 Da d Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline a Missed n Wrnnn Peak PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Pnak II'li'Xi I 4.10 PFUnA 1 563.0/519.0 Da Baseline d Missed ___n Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da Baseline a Missed n Wrnnn Pnak 4.11 PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da o Baseline Missed n Wrnnn Ppak 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak___ PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed Wrnnn Peak 4.76 PFDoA 1 613.0/569.0 Da c Baseline Missed r, Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed Wrnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak I''.A if/ PFTeA 1 713.0/669.0 Da Baseline o Missed ___n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak___ Before After 102 Page 184 of 242 p. 52 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation K1000968-008 !/, : .- ' ."4 in (|pN^ _ 1.38 k ........ PFOA2 413.0/369.0 Da c Baseline Missed Wrong Peak j`,,m A 3S 40 4 S PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak 2.34 IS ;ij 25 30 PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak tsooc v mono s,:, 3 5 4 0 45 SO PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak 1<M P \2f.37 ......... tr m r PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak >noo . 400,1 i&'ii 40 4 A3 45 SO SS PFDoA2 613.0/169.0 Da c Baseline Missed Wrong Peak * 1*4 ;*5 3A1 iCi i s *r> PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak 20) 1500 = 1000 500 516 PFTrA 2 663.0/169.0 Da Baseline Miss Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After 103 Page 185 of 242: p. 53 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-008 2/9/2010 12:01:51 PM PFCkinetexShort.dam 28 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 13C-PFHXA PFHxS1 180-PFHS S J 8 0 -P F H S 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 4467300 5395800 3450700 5395800 5395800 3450700 4419900 4419900 Analyte RT (ExpRT) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.30 (6.32) 6.30 (6.31) 7.64 (7.65) Analyte Area 215830 538450 433680 570910 134170 1547200 3450700 4419900 Calc. Cone (ng/mL) 0.40 1.02 0.37 0.93 0.58 1.34 0.89 1.00 Modified? No No No No No No No No 104 f l 1? " ( t o Page 165 of 245 Services' p. 54 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.26(1.27) 3.59 (3.60) 4.25 (4.26) 5.29(5.31) 6.21 (6.22) 6.30 (6.32) 1.26 (1.26) 5.30(5.30) Area 215830 538450 164380 50497 454720 591350 4467300 5395800 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.38(0.40) YES No 0.09(0.10) YES No 3.39(3.03) YES No 0.38(0.40) YES No 20.70(13.55) No No 9.45 (13.06) No 105 Page 166 of 245 Quantitative Peak Review p. 55 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-008 PFBA 1 213.0/169.0 Da Baseline c Missed n Wrnnn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak 6.?0 PFPeA 1 263.0/219.0 Da Baseline Missed n Wrnnn Peak 13C-PFHXA 315.0/270.0 Da Baseline o Missed n Wrnnn Peak PFBS 1 299.0/80.0 Da Baseline q Missed n Wrnnn Peak 621 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak PFHxA 1 313.0/269.0 Da Baseline o Missed n Wrnnn Peak 13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHpA 1 363.0/169.0 Da o Baseline Missed n Wrnnn Peak 6.30 130-PFHxA 315.0/270.0 Da d Baseline a Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da q Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline c Missed n Wrnnn Peak S J 8 0 -P F H S 403.0/103.0 Da d Baseline Missed n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da Baseline Missed ___r. Wrnnn Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed ____ n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da a Baseline Missed ____ n Wrnnn Peak___ 106 Page 167 of 245 p. 56 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation .______ 126_______ -I iv '"i Ju v i .............. L_ : ::i i ........3.59 -- ............... - ............. 1 K1000968-008 PFBA 2 213.0/169.0 Da c Baseline o Missed o Wrong Peak 6.21 PFPeA2 263.0/219.0 Da Baseline o Missed Wrong Peak 1 1M 1*5 if- 6.B0 A| ......... iu 6$ ro PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak i2 mV; 2 4IMS :<>5 55 \ n0 65 ?1 PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Tte image is rot avabble PFHpA2 363.0/319.0 Da Baseline Missed c Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da c Baseline Missed Wrong Peak N/A Baseline u Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After 107 Page 168 of 245 Colum bia p. 57 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810b\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-009 2/9/2010 1:11:08 AM PFCkinetexLong.dam 29 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 130-PFDoA IS Area 3233800 2177800 5063600 2591600 98970 5063600 105760 105760 105760 Analyte RT (Exp RT ) 1.41 (1.36) 2.39 (2.42) 2.38 (2.53) 3.35 (3.34) 4.12 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 260400 21916 1198800 10391 6359 0 0 0 0 Calc. Cone (ng/mL) 1.690 0.112 2.240 0.067 0.046 N/A N/A N/A N/A Modified? No No No No No No No No No 108 *7 10 (Ur Page 186 of 242 lursibia p. 58 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDOA PFTrA 2 PFTeA2 RT (Exp RT ) 1.40 (1.36) 1.41 (1.36) 2.38 (2.44) 2.33 (2.44) 2.38 (2.53) 2.37 (2.53) 0.00 (3.34) 3.34 (3.34) 0.00 (4.06) 4.13(4.06) 3.91 (4.10) 4.75 (4.64) 4.75 (4.64) 5.21 (5.13) 5.54 (5.56) Area 556170 3233800 6006 2177800 368240 5063600 0 2591600 0 98970 500 1472 105760 1092 1114 Modified? Calc (Exp) MRM Ratio No 2.14(2.38) Ratio Confirms ID YES No 12.42(50.17) No No 0.27 (0.28) YES No 99.37(19.17) No No 0.31 (0.32) YES No 4.22 (22.53) No No 0.00(0.15) No No 249.41 (22.10) No No 0.00 (0.22) No No 15.56(1.86) No No 0.00(0.32) No No 0.00(0.29) No No 0.00 (3.08) No No 0.00(0.32) No No 0.00 (0.36) No 109 Page 187 of 242 p. 59 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review ,,,,,,l 1.41 A PFOA 1 413.0/169.0 Da o Baseline a Missed n Wrnnn Peak ,,,j 2.38 | o5 to rlin#nwi5 :a 13C-PFOA 417.0/372.0 Da c Baseline Missed n Wrnnn Peak 5*5 I 'l -n ,f li pfo sT 499.0/80.0 Da Baseline Missed n Wrnnn Peak :J wj 4.12 Iii :o iu 13C-PFOS 503.0/80.0 Da c Baseline Missed n Wrnnn Peak .'0000 iwao I? lflOtHj K1000968-009 6e4 5<>4 * 404 S .Vi4 14 2.39 Oof Z- <5*5 I 105 S44 I PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak :5o<jo Iion 1 luODO soi <5 3.35 )0 55 40 Tmifei.i, 13C-PFNA 468.0/423.0 Da C Baseline d Missed n Wrnnn Peak 3^ ^ 35 I PFDA1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak -WOO (V 15004 5000 45 t J,S 1*5 I PFUnAI 563.0/519.0 Da u Baseline Missed n Wrnnn Peak * ICW iuhu 13C-PFUnA 565.0/270.0 Da a Baseline Missed n Wrnnn Peak .'W0 4.^5 jWifj f 15444 | lWJfJ MOI PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak MOO S 1500 1 1004 500i 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak 4 .^ f&.. roooo 15444 | 10000 OWO 40 45 50 55 45 50 55 54 PFDoAl 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da c Baseline Missed n Wrnnn Peak ricco PFTrA 1 663.0/619.0 Da Baseline o Missed n Wrnnn Peak_____ 13C-PFDOA 615.0/169.0 Da Baseline o Missed n Wrnnn Peak_____ i *Ji - ;~l ' ;V; v' t*- \*,Wi r i'lfl 4f f. V. 1W0 '' I PFTeA 1 713.0/669.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak Before After 110 Page 188 of 242 p. 60 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation 1.40 PFOA2 413.0/369.0 Da d Baseline o Missed Wrong Peak '*1 2.38 PFNA2 463.0/219.0 Da g Baseline Missed Wrong Peak K1000968-009 PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da o Baseline Missed Wrong Peak PFUnA2 563.0/269.0 Da c Baseline Missed Wrong Peak .. PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da c Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 189 of 242 111 p. 61 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 OtSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-009 2/9/2010 12:11:24 PM PFCkinetexShort.dam 29 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 4570700 5588000 3415200 5588000 5588000 3415200 4747500 4747500 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.30 (6.32) 6.30 (6.31) 7.64 (7.65) Analyte Area 201720 564890 443060 588490 141050 1538900 3415200 4747500 Calc. Cone (ng/mL) 0.36 1.04 0.39 0.93 0.59 1.35 0.82 1.00 Modified? No No No No No No No No 112 i / V* (> 2 (s Page 169 of 245 p. 62 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.26(1.27) 3.59(3.60) 4.25(4.26) 5.30(5.31) 6.21 (6.22) 6.30(6.32) 1.26 (1.26) 5.30(5.30) Area 201720 564890 161230 50142 468340 603270 4570700 5588000 Modified? Calc (Exp) MRM Ratio No 1.00 (1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.36 (0.40) YES No 0.09(0.10) YES No 3.32(3.03) YES No 0.39(0.40) YES No 22.66(13.55) No No 9.50(13.06) No 113 Page 170 of 245 Quantitative Peak Review p. 63 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-009 Before After 114 Page 171 of 245 Sw m w s p. 64 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation "T 1.26 - >-r u- Iw \ 3^ K1000968-009 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak 6.21 PFPeA2 263.0/219.0 Da Baseline u Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline c Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline a Missed Wrong Peak 6. |G- 5 HSfS V5 isA S 6(i '1 PFHpA2 363.0/319.0 Da Baseline u Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak SJ3C-PFDA 515.0/470.0 Da Baseline Missed g Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Thisinaae isrd avaisble N/A Baseline Missed Wrong Peak Before After 115 Page 172 of 245 p. 65 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 OtASamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-010 2/9/2010 1:18:43 AM PFCkinetexLong.dam 30 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3245500 2302400 5174400 2271400 102400 5174400 117800 117800 117800 Analyte RT (Exp RT ) 1.35 (1.36) 2.31 (2.42) 2.35 (2.53) 0.00(3.34) 4.14 (4.06) 4.14 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74) Analyte Area 772580 45488 6386400 0 8043 6480 0 0 0 Calc. Cone (ng/mL) 4.930 0.196 11.600 N/A 0.061 0.031 N/A N/A N/A Modified? No No No No No No No No No 116 t'' y\. *7 (10 IU Page 190 of 242 p. 66 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name RT (Exp R T ) PFOA2 1.35(1.36) S_13C_PFOA 1.35(1.36) PFNA2 2.32 (2.44) S_13C_PFNA 2.31 (2.44) PFOS2 2.35 (2.53) S_13C-PFOS 2.36(2.53) PFDA 2 0.00 (3.34) S_13C-PFDA 3.34 (3.34) PFUnA2 0.00 (4.06) S_13C-PFUnA 4.13(4.06) PFDS 2 . 4.14(4.10) PFDoA2 4.77 (4.64) S_13C-PFDoA 4.77 (4.64) PFTrA 2 5.20(5.13) PFTeA2 5.72(5.56) l_______________________ Area 1651500 3245500 14825 2302400 2386700 5174400 0 2271400 0 102400 2039 2079 117800 346 255 Modified? Calc (Exp) MRM Ratio No 2.14(2.38) Ratio Confirms ID YES No 4.20 (50.17) No No 0.33(0.28) YES No 50.62(19.17) No No 0.37(0.32) YES No 0.81 (22.53) No No 0.00(0.15) No No 0.00 (22.10) No No 0.00 (0.22) No No 12.73 (1.86) No No 0.31 (0.32) YES No 0.00 (0.29) No No 0.00(3.08) No No 0.00 (0.32) No No 0.00 (0.36) No 117 Page 191 of 242 Quantitative Peak Review p. 67 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000968-010 PFOA 1 413.0/169.0 Da o Baseline Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed Wrnnn Peak PFNA 1 463.0/419.0 Da d Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed ___n Wrnnn Peak___ PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Ppak 4.14 i l \ /',i PFUnA 1 563.0/519.0 Da cj Baseline Missed ___n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Pnak PFDA 1 513.0/469.0 Da Baseline a Missed n Wrnnn Peak 8000 7m ,, wo I f^ SOdO i 3000 4.14 1000 ...... ......... A________ it -JO -JS 1(1 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak____ PFDS 1 599.0/80.0 Da a Baseline d Missed n Wrnnn Peak_____ 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak 1-5 13C-PFOS 503.0/80.0 Da n Wrnnn Peak , v,;Vvil PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Pnak 13C-PFDoA 615.0/169.0 Da Baseline Missed n Wrnnn Pnak PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak . ;.>:Mjit h;! ` <\h,` i.` Yi ` "A:-f PFTeA 1 713.0/669.0 Da Baseline Missed ___ n Wrnnn Peak 13C-PFDoA 615.0/169.0 Da c Baseline Missed ___ n Wrnnn P e a k __ Before After 118 Page 192 of 242 p. 68 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation u.,,j 1 1.35 i ........... 1 1 ic.rjnn| i. j t! - SDuj LA 15 ZD .'5 30 PFOA2 413.0/369.0 Da l Baseline Missed a Wrong Peak PFNA2 463.0/219.0 Da Baseline d Missed Wrong Peak PFUnA 2 563.0/269.0 Da Baseline Missed Wrong Peak -m .,, ... IS JO J S Si] PFDS~2 599.0/99.0 Da Baseline Missed Wrong Peak K1000968-010 .... 1 1 f \ 2l3\ .'>) l l :Ls iri SWli i<|ir 1000 XX * 3000 IttXi :5 IS JO PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed o Wrong Peak - soo 4,p7 - a . <0 4S SO ">S PFDoA 2 613.0/169.0 Da Baseline Missed Wrong Peak 0U "tt UX) 4^ 1M CuO li 4S 5 S r, t. >' PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak ,,r - W k .- . -, PFTeA 2 713.0/169.0 Da c Baseline Missed o Wrong Peak Before After 119 Page 193 of 242 p. 69 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-010 2/9/2010 12:20:57 PM PFCkinetexShort.dam 30 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 130-PFHxA PFHxS1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 4480700 5771500 3272200 5771500 5771500 3272200 4581000 4581000 Analyte RT (Exp RT ) 1.26 (1.27) 3.58 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 532650 1399700 1008800 1801500 357920 6884400 3272200 4581000 Calc. Cone (ng/mL) 1.07 2.52 0.89 2.88 1.54 6.53 0.81 1.00 Modified? No No No No No No No No 120 z n<, I Page 173 of 245 p. 70 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.26(1.27) 3.58(3.60) 4.25 (4.26) 5.29(5.31) 6.21 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30) Area 532650 1399700 376670 173940 1037900 2687400 4480700 5771500 Modified? No Calc (Exp) MRM Ratio 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.37(0.40) YES No 0.10(0.10) YES No 2.90(3.03) YES No 0.39(0.40) YES No 8.41 (13.55) No No 3.20(13.06) No Page 174 of 245 121 im i Srvk$" Quantitative Peak Review 1.26 p. 71 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-010 5.30 PFBA 1 213.0/169.0 Da c Baseline Missed i ' Wronn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak PFPeA 1 263.0/219.0 Da Baseline Missed n Wronn Peak 13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFBS 1 299.0/80.0 Da D Baseline Missed h Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Pnak PFHxA 1 313.0/269.0 Da d Baseline Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Psak 6.; PFHpA 1 363.0/169.0 Da o Baseline g Missed n Wrnnn Peak 6.31 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak 7.64 PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da g Baseline u Missed n Wrnnn Peak 1 S J 8 0 -P F H S 403.0/103.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da g Baseline Missed ___n Wrnnn Peak___ SJ3C-PFDA 515.0/470.0 Da q Baseline Missed ___ p- W rnnaP e ak___ Before After 13C-PFDA 515.0/470.0 Da c Baseline Missed ___ n Wrnnn Peak___ 122 Page 175 of 245 Services* Ion Ratio Confirmation 1.26 3.58 Created with Analyif ^ p o rte r Printed: 15/02/2010 2:34:00 PM K1000968-010 PFBA2 213.0/169.0 Da a Baseline a Missed Wrong Peak 6.21 I PFPeA2 263.0/219.0 Da Baseline a Missed Wrong Peak PFBS2 299.0/99.0 Da d Baseline a Missed Wrong Peak PFHpA2 363.0/319.0 Da Baseline c Missed d Wrong Peak PFHxS2 399.0/99.0 Da g Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da g Baseline Missed g Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da d Baseline d Missed Wrong Peak This rnage is rd avafeble N/A c Baseline Missed c Wrong Peak 123 Page 176 of 245 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-011 2/9/2010 1:26:18 AM PFCkinetexLong.dam 31 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 130-PFDoA IS Area 2812400 2166900 5555000 2291100 94733 5555000 126630 126630 126630 Analyte RT (Exp RT ) 1.41 (1.36) 2.36 (2.42) 2.40 (2.53) 0.00 (3.34) 4.16 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 1843200 71582 9557300 0 4372 0 0 0 0 Calc. Cone (ng/mL) 13.500 0.312 16.200 N/A 0.027 N/A N/A N/A N/A Modified? No No No No No No No No No 124 (Uo( ( & Is Page 194 of 242 p. 74 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFD0A PFTrA 2 PFTeA2 RT (Exp RT ) 1.41 (1.36) 1.41 (1.36) 2.36 (2.44) 2.33 (2.44) 2.40 (2.53) 2.41 (2.53) 0.00 (3.34) 3.36 (3.34) 0.00(4.06) 4.14(4.06) 4.33 (4.10) 4.79 (4.64) 4.77 (4.64) 5.18(5.13) 5.41 (5.56) Area 4268400 2812400 20657 2166900 3169900 5555000 0 2291100 0 94733 157 1214 126630 457 332 Modified? Calc (Exp) MRM Ratio No 2.32(2.38) Ratio Confirms ID YES No 1.53 (50.17) No No 0.29(0.28) YES No 30.27(19.17) No No 0.33 (0.32) YES No 0.58 (22.53) No No 0.00 (0.15) No No 0.00 (22.10) No No 0.00 (0.22) No No 21.67(1.86) No No 0.00 (0.32) No No 0.00(0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No 125 Page 195 of 242 Columbia p. 75 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review 1.41 1.41 K1000968-011 I PFOA 1 413.0/169.0 Da Baseline Missed n Wrnnn Peak 2.40 1 PFOS T 499.0/80.0 Da o Baseline a Missed n Wrnnn Peek o s in 13C-PFOA 417.0/372.0 Da Baseline a Missed ___n Wrnnn Peak 2.41 vs i :: U5 1 13C-PFOS 503.0/80.0 Da Baseline a Missed n Wrnnn Ppak PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak ! I! PFDA 1 513.0/469.0 Da d Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline d Missed n Wrnnn Pnak |4.16 PFUnA 1 563.0/519.0 Da Baseline Missed ___n Wrnnn Peak___ 13C-PFUnA 565.0/270.0 Da Baseline Missed Wrnnn Psak PFDS 1 599.0/80.0 Da q Baseline Missed n Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da Baseline Missed Wrnnn Peak PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak a-S 13C-PFDOA 615.0/169.0 Da q Baseline Missed n W rnnn Peak jl rs PFTrAI 663.0/619.0 Da Baseline Missed n Wrnnn Peak___ 1 13C-PFDOA 615.0/169.0 Da Baseline Missed ___ n Wrnnn Peak___ PFTeA 1 713.0/669.0 Da c Baseline Missed ___ n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da o Baseline Missed ___ n Wrnnn Peak___ Before After 126 Page 196 of 242 p. 76 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation J 1.41 l UiGOti 2A> f -'rt K1000968-011 A / \ 2 4V SGI ? KM: wco llW|l PFOA2 413.0/369.0 Da o Baseline Missed d Wrong Peak <r. i Cl 4 5 PFUnA2 563.0/269.0 Da Baseline Missed c Wrong Peak PFNA 2 463.0/219.0 Da Baseline Missed Wrong Peak Wi | i "1 ""i 4.33 IS 40 45 50 PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da Baseline Missed a Wrong Peak iyio !-5C0 I SuO PFDoA 2 613.0/169.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da c Baseline Missed Wrong Peak >00 MO "i 45 5> 55 r.C PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da c Baseline Missed Wrong Peak Before After 127 Page 197 of 242 p. 77 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-011 2/9/2010 12:30:32 PM PFCkinetexShort.dam 31 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 4176700 5115000 3288500 5115000 5115000 3288500 4121500 4121500 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 882530 3136300 2690400 3957200 721300 18996000 3288500 4121500 Calc. Cone (ng/mL) 1.94 6.40 2.36 7.32 3.58 19.50 0.91 1.00 Modified? No No No No No No No No 128 Page 177 of 245 Services' p. 78 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.26 (1.27) 3.59(3.60) 4.25 (4.26) 5.30(5.31) 6.21 (6.22) 6.31 (6.32) 1.26 (1.26) 5.30(5.30) Area 882530 3136300 1006500 376440 2284300 8001900 4176700 5115000 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.37(0.40) YES NO 0.10(0.10) YES No 3.17(3.03) YES No 0.42(0.40) YES No 4.73 (13.55) No No 1.29(13.06) No 129 Page 178 of 245 p. 79 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review 1.26 K1000968-011 | 1dG L Is 5.30 :: k 1 ........... 1- id I 11 in HI I75 4<! . .45. i ; ii| PFBA 1 213.0/169.0 Da Baseline Missed _____ [-Wrnnn Peak ___ 13C-PFBA 217.0/172.0 Da c Baseline Missed n Wrnnn Peak s; 6.31 *5 SS all 55 70 PFPeA 1 263.0/219.0 Da Baseline Missed _____ n Wrnnn Peak_____ &o*c h l4 45 501 55 b'l 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak t4*i. 4.1*5 I PFBS 1 299.0/80.0 Da c Baseline Missed n Wrnnn Peak 6.^2 18 0 -P F H S 403.0/103.0 Da G Baseline o Missed n Wrnnn Peak ,W1 5.30 , '** b Stef, |- 45 55 a PFHxA 1 313.0/269.0 Da a Baseline d Missed n Wrnnn Peak 6.31 f ISti i . __ 13C-PFHxA 315.0/270.0 Da G Baseline o Missed n Wrnnn Peak I PFHpA 1 363.0/169.0 Da a Baseline u Missed n Wrnnn Peak I 6.31 13C-PFHxA 315.0/270.0 Da c Baseline o Missed n Wrnnn Peak 7.^4 PFHxS 1 399.0/80.0 Da a Baseline Missed n Wrnnn Peak 7.^4 18 0 -P F H S 403.0/103.0 Da Baseline Missed n Wrnnn Peak 7.^4 `1 [ a 0*5 | l'U'J '0*5 ?o ft a? a 1ter> ; >ite5 4(#fi a0t5 711 75 -50 3? S 180-PFHS 403.0/103.0 Da a Baseline Missed _____ n, Wrnnn Peak_____ 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak_____ 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak_____ Before After 130 Page 179 of 245 Ion Ratio Confirmation 1.26 3.59 p. 80 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-011 PFBA2 213.0/169.0 Da Baseline Missed c Wrong Peak 6.21 PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak I 6. ~ - -] 5 _____________ L _ ____ PFHpA2 363.0/319.0 Da Baseline Missed c Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da c Baseline Missed Wrong Peak IMB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Tits imageisnot avaiahle N/A Baseline u Missed Wrong Peak Page 180 of 245 131 p. 81 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-012 2/9/2010 1:33:52 AM PFCkinetexLong.dam 32 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFD0A IS Area 3408400 2361500 5022700 2448400 81066 5022700 115090 115090 115090 Analyte RT (Exp RT ) 1.42 (1.36) 2.39 (2.42) 2.42 (2.53) 3.37 (3.34) 4.16 (4.06) 0.00(4.10) 0.00 (4.63) 0.00 (5.14) 0.00(5.74) Analyte Area 84385 27431 616810 11948 3304 0 0 0 0 Calc. Cone (ng/mL) 0.544 0.125 1.170 0.075 0.021 N/A N/A N/A N/A Modified? No No No No No No No No No A \ \ &V \ 7 ,t ( ( ^ ( < 132 Page 198 of 242 SrkSw Created with Analyst' Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFD0A PFTrA 2 PFTeA2 RT (Exp R T ) 1.42(1.36) 1.44(1.36) 2.37 (2.44) 2.40 (2.44) 2.43 (2.53) 2.42 (2.53) 0.00 (3.34) 3.37 (3.34) 0.00 (4.06) 4.15(4.06) 4.02 (4.10) 4.58(4.64) 4.78 (4.64) 5.29(5.13) 5.74(5.56) Area 197560 3408400 7926 2361500 195150 5022700 0 2448400 0 81066 362 647 115090 560 174 Modified? Calc (Exp) MRM Ratio No 2.34(2.38) Ratio Confirms ID YES No 40.39(50.17) YES No 0.29(0.28) YES No 86.09(19.17) No No 0.32 (0.32) YES No 8.14(22.53) No No 0.00(0.15) No No 204.92 (22.10) No No 0.00 (0.22) No No 24.53 (1.86) No No 0.00 (0.32) No No 0.00(0.29) No No 0.00(3.08) No No 0.00 (0.32) No No 0.00 (0.36) No 133 Page 199 of 242 p. 83 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review 1.42 1 PFOA 1 413.0/169.0 Da q Baseline c Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed Wronn Psak K1000968-012 2,39 i PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Peak PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak 4.16 13C-PFOS 503.0/80.0 Da a Baseline Missed n Wrnnn Peak PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed Wrnnn Peak I'Vi.-'v V *1 if ? PFUnA 1 563.0/519.0 Da Baseline q Missed ___n Wrnnn Peak___ PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFUnA PFDS 1 13C-PFOS 565.0/270.0 Da 599.0/80.0 Da 503.0/80.0 Da Baseline Missed Wrnnn Peak 4.^8 Baseline a Missed n Wrnnn Peak im 7(10 Baseline Missed i Wrnnn Peak IVKIII ! i iso n 4T!O '* :woo 100 ` .fjOS 0 *0 JS 5 5$ Si) Time mir> SS <-0 13C-PFDOA PFTrA 1 13C-PFDOA 615.0/169.0 Da 663.0/619.0 Da 615.0/169.0 Da Baseline Missed n Wrnnn Peak Baseline Missed n Wrnnn Peak_____ Baseline c Misse n Wrnnn Peak " ] v. .i* A A k !j !\ t PFTeA 1 713.0/669.0 Da d Baseline c Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed ___n Wrnnn Peak___ Before After 134 Page 200 of 242 p. 84 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation 1.42 i PFOA2 413.0/369.0 Da d Baseline u Missed c Wrong Peak PFNA2 463.0/219.0 Da a Baseline Missed Wrong Peak K1000968-012 PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da a Baseline Missed Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline o Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline d Missed Wrong Peak PFTeA2 713.0/169.0 Da l: Baseline Missed Wrong Peak Before After 135 Page 201 of 242 p. 85 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-012 2/9/2010 12:40:07 PM PFCkinetexShort.dam 32 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS SJ80-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 4458600 5326900 3497100 5326900 5326900 3497100 4777100 4777100 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 77293 99356 164120 136190 42744 595660 3497100 4777100 Calc. Cone (n g /m l) 0.11 0.17 0.15 0.18 0.15 0.50 0.83 1.00 Modified? No No No No No No No No 136 Page 181 of 245 .................................................. f" l C o lu m b ia Analytical Services- p. 86 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT) 1.26(1.27) 3.59 (3.60) 4.25 (4.26) 5.30(5.31) 6.21 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30) Area 77293 99356 58366 13424 376370 223850 4458600 5326900 Modified? No Calc (Exp) MRM Ratio 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.36(0.40) YES No 0.10(0.10) YES No 8.81 (3.03) No No 0.38(0.40) YES No 57.68(13.55) No No 39.11 (13.06) No 137 Page 182 of 245 p. 87 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review PFBA 1 213.0/169.0 Da Baseline c Missed n Wrnnn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak PFBS 1 299.0/80.0 Da Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Ppak K1000968-012 <6* 5.30 3.59 & net s to* 1 .............. _L PFPeA 1 263.0/219.0 Da Baseline Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da d Baseline Missed n Wrnnn Peak 1SS 5.30 PFHxA 1 313.0/269.0 Da Baseline d Missed n Wrnnn Pnak _*6i- 1OtS S SO*, L SO 5 ? 60 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHpA 1 363.0/169.0 Da g Baseline o Missed i i Wrnnn Pftak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da o Baseline a Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline a Missed Wrnnn Peak S J 8 0 -P F H S 403.0/103.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da Baseline Missed ____ n Wrnnn Peak___ S_13C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Pnak 138 Page 183 of 245 $fie5' Created with AnalysPReporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation K1000968-012 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak ... -- -A-; ______ ---- _ PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak Tnw mn PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da a Baseline a Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak 139 Page 184 of 245 p. 89 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-013 2/9/2010 1:41:27 AM PFCkinetexLong.dam 33 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFD0A PFTeA 1 13C-PFDOA IS Area 3239800 2372200 4992700 2379200 91465 4992700 116340 116340 116340 Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.40 (2.53) 0.00(3.34) 4.14 (4.06) 0.00(4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74) Analyte Area 0 0 22145 0 4283 0 0 0 0 Calc. Cone (ng/mL) N/A N/A 0.056 N/A 0.028 N/A N/A N/A N/A Modified? No No No No No No No No No S0 7 (y 4 U (It 140 Page 202 of 242 p. 90 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp R T ) 1.39(1.36) 1.38 (1.36) 2.55 (2.44) 2.35 (2.44) 2.43 (2.53) 2.41 (2.53) 0.00 (3.34) 3.37 (3.34) 0.00 (4.06) 4.16(4.06) 4.12 (4.10) 4.78 (4.64) 4.79 (4.64) 5.22(5.13) 5.70 (5.56) Area 8943 3239800 286 2372200 4812 4992700 0 2379200 0 91465 255 1029 116340 431 298 Modified? Calc (Exp) MRM Ratio No 0.00 (2.38) Ratio Confirms ID No No 0.00(50.17) No No 0.00(0.28) No No 0.00(19.17) No No 0.22(0.32) No No 225.45 (22.53) No No 0.00 (0.15) No No 0.00 (22.10) No No 0.00 (0.22) No No 21.36(1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00(0.32) No No 0.00 (0.36) No Page 203 of 242 141 Quantitative Peak Review p. 91 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000968-013 Before After 142 Page 204 of 242 p. 92 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation /. ; '' j: 1.39 PFOA2 413.0/369.0 Da c Baseline Missed o Wrong Peak j f,"U j |lOtlG '|W\ is u :`j do PFNA2 463.0/219.0 Da o Baseline Missed Wrong Peak K1000968-013 PFOS2 499.0/99.0 Da o Baseline o Missed Wrong Peak PFDA2 513.0/269.0 Da c Baseline Missed q Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak PFDS 2 599.0/99.0 Da Baseline Missed o Wrong Peak PFDoA2 613.0/169.0 Da Baseline o Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed o Wrong Peak PFTeA2 713.0/169.0 Da d Baseline Missed Wrong Peak Before After 143 Page 205 of 242 p. 93 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff Q20810SamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-013 2/9/2010 12:49:39 PM PFCkinetexShort.dam 33 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5015100 6017900 3322600 6017900 6017900 3322600 4580700 4580700 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 0.00 (4.26) 5.30 (5.31) 6.23 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 57177 55157 0 61223 13783 45582 3322600 4580700 Calc. Cone (ng/mL) 0.05 0.08 N/A 0.04 0.00 0.03 0.82 1.00 Modified? No No No No No No No No (CO ' U i l<* 144 Page 185 of 245 p. 94 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.26 (1.27) 3.59(3.60) 0.00 (4.26) 5.31 (5.31) 6.23 (6.22) 6.30(6.32) 1.26(1.26) 5.30 (5.30) Area 57177 55157 0 5587 169520 17952 5015100 6017900 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.00(0.40) No No 0.09(0.10) YES No 12.30 (3.03) No No 0.39(0.40) YES No 87.71 (13.55) No No 98.29 (13.06) No 145 Page 186 of 245 p. 95 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review 1.26 11, PFBA 1 213.0/169.0 Da Baseline c Missed n Wrnnn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak PFBS 1 299.0/80.0 Da Baseline Missed r Wrnnn Peak 6.23 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak K1000968-013 PFPeA 1 263.0/219.0 Da Baseline Missed n Wrnnn Peak 5.30 i PFHxA 1 313.0/269.0 Da Baseline Missed Wrnnn Pnak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Pnak 1 c.*c 5.30 8 t to, r.0S . 1 13C-PFHXA 315.0/270.0 Da Baseline Missed Wrnnn Pnak PFHpA 1 363.0/169.0 Da Baseline Missed n Wrnnn Peak 13C-PFHXA 315.0/270.0 Da Baseline Missed Wrnnn Peak PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline Missed p Wrnnn Peak S_180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Pe.ak 13C-PFDA 515.0/470.0 Da Baseline Missed ___n Wrnnn Peak___ S_13C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrnnn Peak___ 146 Page 187 of 245 Ion Ratio Confirmation Created with Analyif ^Iporter Printed: 15/02/2010 2:34:00 PM K1000968-013 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak PFHpA2 363.0/319.0 Da Baseline Missed o Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A o Baseline Missed o Wrong Peak 147 Page 188 of 245 p. 97 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 02081 Ob\SampiesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-014 2/9/2010 1:49:02 AM PFCkinetexLong.dam 34 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3297400 2413300 5356000 2308700 103190 5356000 114810 114810 114810 Analyte RT (Exp RT ) 1.40 (1.36) 2.36 (2.42) 2.40 (2.53) 0.00 (3.34) 4.18 (4.06) 0.00(4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 136270 15947 615290 0 3864 0 0 0 0 Calc. Cone (ng/mL) 0.885 0.082 1.090 N/A 0.018 N/A N/A N/A N/A Modified? No No No No No No No No No 148 y if(( if Page 206 of 242 iu m bia p. 98 Created with Analyst Reporter Printed: 14/02/2010 6 41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDOA PFTrA 2 PFTeA2 RT (Exp R T ) 1.41 (1.36) 1.39(1.36) 2.38 (2.44) 2.36 (2.44) 2.40 (2.53) 2.40 (2.53) 0.00 (3.34) 3.37(3.34) 0.00 (4.06) 4.15(4.06) 4.16 (4.10) 4.79 (4.64) 4.79 (4.64) 5.34 (5.13) 5.46 (5.56) Area 331380 3297400 5457 2413300 207580 5356000 0 2308700 0 103190 1006 1780 114810 453 498 Modified? Calc (Exp) MRM Ratio No 2.43 (2.38) Ratio Confirms ID YES No 24.20(50.17) No No 0.34(0.28) YES No 151.33(19.17) No No 0.34(0.32) YES No 8.70 (22.53) No No 0.00(0.15) No No 0.00 (22.10) No No 0.00 (0.22) No No 26.70(1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No 149 Page 207 of 242 p. 99 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review 1.40 K1000968-014 L PFOA 1 413.0/169.0 Da o Baseline Missed n Wrnnn Peak 2.40 13C-PFOA 417.0/372.0 Da Baseline Missed n Wronn Peak 2.4 PFNA 1 463.0/419.0 Da o Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline a Missed Wronn Peak L PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak 4.18 i PFUnA 1 563.0/519.0 Da Baseline Missed ___'' Wrnnn Peak___ i 13C-PFOS 503.0/80.0 Da o Baseline Missed n Wrnnn Pnak 4.35 :xio 'i ".nflC 5. L5WI0 _________________________ 13C-PFUnA 565.0/270.0 Da o Baseline Missed __ p Wmm PftaK__ 4.79 PFDA 1 513.0/469.0 Da Baseline o Missed n Wrnnn Pnak PFDS 1 599.0/80.0 Da a Baseline Missed ___ n Wrnnn Peak l v ''' :J ' j PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da u Baseline Missed n Wrnnn Peak 4.79 i!!'*!'"V'*/I\`!1,A'lll f i. PFTrA 1 663.0/619.0 Da Baseline Missed ____n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Ppak j 2.40 sL 13C-PFOS 503.0/80.0 Da Baseline Missed ___n Wrnnn Peak 4.79 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Pnak ;"t 'V r /W-'-vVV'.;v7i"V r PFTeA 1 713.0/669.0 Da o Baseline Missed p Wrnnn Peak 130-PFDoA 615.0/169.0 Da Baseline o Missed ___n Wrnnn Peak___ Before After 150 Page 208 of 242 S^n/ices Ion Ratio Confirmation 1,41 p. 100 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000968-014 1 PFOA2 413.0/369.0 Da c Baseline Missed c Wrong Peak PFUnA 2 563.0/269.0 Da u Baseline o Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak 4.16 PFDS2 599.0/99.0 Da Baseline o Missed Wrong Peak PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak i; t-uo i. Wyv PFDoA 2 613.0/169.0 Da G Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed g Wrong Peak K'M00 ^ too ? Ly 1Ofi fB -W M 45 IS 55 ! fen PFTrA2 663.0/169.0 Da Baseline Missed c Wrong Peak PFTeA2 713.0/169.0 Da c Baseline Missed Wrong Peak Before After Page 209 of 242 151 m rm m s" p. 101 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 02081 OVSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-014 2/9/2010 12:59:12 PM PFCkinetexShort.dam 34 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 4320800 5453900 3623200 5453900 5453900 3623200 4274300 4274300 Analyte RT (Exp RT ) 1.27 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 126780 276260 287430 345890 82017 1399500 3623200 4274300 Calc. Cone (ng/mL) 0.22 0.51 0.24 0.54 0.33 1.15 0.96 1.00 Modified? No No NO No No No No No 152 Page 189 of 245 p. 102 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.27(1.27) 3.59(3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.22) 6.31 (6.32) 1.26 (1.26) 5.30 (5.30) Area 126780 276260 111570 31884 254880 544310 4320800 5453900 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.39(0.40) YES No 0.09(0.10) YES No 3.11 (3.03) YES No 0.39(0.40) YES No 34.08 (13.55) No No 15.77(13.06) No 153 Page 190 of 245 /% Columbia W v J Analytical Services" Quantitative Peak Review p. 103 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-014 Before After 154 Page 191 of 245 p. 104 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation : 3.59 r K1000968-014 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak ,,t - .6.22 . . . ) ... r.{" ' I J >s 6. [ I' - ` " s j .... .,.- '* 4 . 55 bfl eS .'0 PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak ._6_5___*_6A. t 65 '0 PFHXS2 399.0/99.0 Da Baseline Missed Wrong Peak H 56 60 65 76 S 13C-PFDA 515.0/470.0 Da Baseline c Missed Wrong Peak NB PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak iinifiogeisnoi N/A Baseline Missed Wrong Peak 155 Page 192 of 245 p. 105 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-015 2/9/2010 1:56:34 AM PFCkinetexLong.dam 35 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3284000 2182500 4486700 2253600 75915 4486700 100750 100750 100750 Analyte RT (ExpRT) 1.37 (1.36) 2.36 (2.42) 2.37 (2.53) 0.00 (3.34) 4.15 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00 (5.14) 0.00 (5.74) Analyte Area 154580 17448 551180 0 2692 0 0 0 0 Calc. Cone (ng/mL) 1.000 0.094 1.170 N/A 0.016 N/A N/A N/A N/A Modified? No No No No No No No No No 156 Page 210 of 242 p. 106 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp R T ) 1.37(1.36) 1.36 (1.36) 2.33 (2.44) 2.33 (2.44) 2.38 (2.53) 2.38 (2.53) 0.00 (3.34) 3.36(3.34) 0.00 (4.06) 4.14(4.06) 4.22 (4.10) 4.86 (4.64) 4.78 (4.64) 5.14(5.13) 5.32 (5.56) Area 300840 3284000 4243 2182500 185320 4486700 0 2253600 0 75915 205 705 100750 375 440 Modified? Calc (Exp) MRM Ratio No 1.95(2.38) Ratio Confirms ID YES No 21.24 (50.17) No No 0.24(0.28) YES No 125.09(19.17) No No 0.34(0.32) YES No 8.14(22.53) No No 0.00 (0.15) No No 0.00(22.10) No No 0.00 (0.22) No No 28.20 (1.86) No No 0.00(0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No 157 Page 211 of 242 p. 107 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Quantitative Peak Review J X PFOA 1 413.0/169.0 Da Baseline Missed n Wronn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wronn Peak K1000968-015 2.36 PFNA 1 463.0/419.0 Da d Baseline Missed n Wronn Peak 13C-PFNA 468.0/423.0 Da o Baseline Missed Wronn Peak PFOS 1 499.0/80.0 Da Baseline Missed n Wronn Poak 4.15 13C-PFOS 503.0/80.0 Da d Baseline Missed Wronn Paak PFDA 1 513.0/469.0 Da Baseline Missed n Wronn Paak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wronn Paak PFUnA 1 563.0/519.0 Da o Baseline Missed ___ n Wronn Peak___ 13C-PFUnA 565.0/270.0 Da o Baseline Missed Wronn Peak PFDS 1 599.0/80.0 Da d Baseline Missed n Wronn Peak___ 13C-PFOS 503.0/80.0 Da Baseline Missed Wronn Peak i V-A, PFDoA 1 613.0/569.0 Da u Baseline Missed n Wronn Peak 13C-PFDOA 615.0/169.0 Da o Baseline Missed n Wronn Paak PFTrA 1 663.0/619.0 Da Baseline Missed n Wronn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wronn Paak PFTeA 1 713.0/669.0 Da u Baseline Missed ___ n Wronn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wronn Peak___ Before After 158 Page 212 of 242 p. 108 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation 1.37 K1000968-015 | .0 PFOA2 413.0/369.0 Da Baseline Missed c Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak PFUnA 2 563.0/269.0 Da c Baseline c Missed Wrong Peak 4.22 PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da o Baseline u Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline o Missed Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed c Wrong Peak Before After 159 Page 213 of 242 p. 109 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-015 2/9/2010 1:08:44 PM PFCkinetexShort.dam 35 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 4421200 5546800 3533400 5546800 5546800 3533400 4641000 4641000 Analyte RT (Exp RT ) 1.25 (1.27) 3.58 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 127790 285390 289520 370910 87259 1398000 3533400 4641000 Calc. Cone (ng/mL) 0.22 0.52 0.25 0.57 0.35 1.18 0.86 1.00 Modified? No No No No No No No No 160 Page 193 of 245 p. 110 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.25(1.27) 3.58(3.60) 4.25(4.26) 5.30(5.31) 6.21 (6.22) 6.31 (6.32) 1.27 (1.26) 5.30(5.30) Area 127790 285390 108960 34058 249650 555660 4421200 5546800 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.38(0.40) YES No 0.09(0.10) YES No 2.86(3.03) YES No 0.40(0.40) YES No 34.60(13.55) No No 14.95(13.06) YES Page 194 of 245 161 Quantitative Peak Review p. 111 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-015 Before After 162 Page 195 of 245 p. 112 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 1.25 3.58 K1000968-015 /v/V PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak 6.21 i .. t? 4. 5 ............ PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak | *0 JS so PFBS2 299.0/99.0 Da o Baseline Missed Wrong Peak 1-W. PFHxA 2 313.0/119.0 Da Baseline Misse Wrong Peak ....- : l ............. 6.81 Thisimageisnei wateble 1 PFHpA 2 363.0/319.0 Da Baseline a Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da D Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. N/A Baseline o Missed Wrong Peak Before After 163 Page 196 of 245 um bm p. 113 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's ProjectstPFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-016 2/9/2010 2:04:07 AM PFCkinetexLong.dam 36 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 130-PFDoA PFTrA 1 13C-PFD0A PFTeA 1 130-PFDoA IS Area 3922200 2576500 5693100 2563500 99878 5693100 108150 108150 108150 Analyte RT (Exp RT ) 1.37 (1.36) 2.31 (2.42) 2.38 (2.53) 0.00 (3.34) 4.13 (4.06) 3.82(4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 160180 25687 848150 0 4824 26566 0 0 0 Calc. Cone (ng/mL) 0.875 0.111 1.410 N/A 0.029 0.060 N/A N/A N/A Modified? No No No No No No No No No > Du 164 page 214 of 242 Sm m m s' p. 114 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp R T ) 1.38 (1.36) 1.37(1.36) 2.32(2.44) 2.33 (2.44) 2.40 (2.53) 2.38 (2.53) 0.00(3.34) 3.35(3.34) 0.00 (4.06) 4.14(4.06) 0.00 (4.10) 4.82 (4.64) 4.78 (4.64) 5.27 (5.13) 5.80(5.56) Area 342100 3922200 8621 2576500 278510 5693100 0 2563500 0 99878 0 771 108150 341 102 Modified? Calc (Exp) MRM Ratio No 2.14(2.38) Ratio Confirms ID YES No 24.49(50.17) No No 0.34(0.28) YES No 100.30(19.17) No No 0.33(0.32) YES No 6.71 (22.53) No No 0.00 (0.15) No No 0.00 (22.10) No No 0.00 (0.22) No No 20.70(1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00(3.08) No No 0.00 (0.32) No No 0.00 (0.36) No 165 Page 215 of 242 S' Quantitative Peak Review p. 115 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000968-016 PFOA 1 413.0/169.0 Da d Baseline Missed i <Wrnnn Peak 13C-PFOA 417.0/372.0 Da g Baseline Missed ___n Wrnnn Peak 2.31 PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline g Missed n Wrnnn Peak PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak 4.13 13C-PFOS 503.0/80.0 Da Baseline o Missed n Wrnnn Peak PFDA 1 513.0/469.0 Da Baseline a Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak PFUnA 1 563.0/519.0 Da Baseline Missed __ p Wronn PoaK__ 'V ''/ 7( PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak 4^8 PFDS 1 13C-PFOS 599.0/80.0 Da 503.0/80.0 Da Baseline Missed u Baseline o Missed n Wrnnn Peak 10 r. Wrnnn Peak |7W' (>0 ? 4<in | 'W l\ 1, i. 100 Jji'ii:.:l:ii/\'J * p IWO S1 iruxif) >,,'! 50i!!3 40 45 5o 55 45 50 55 b0 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFTrAI 663.0/619.0 Da Baseline Missed n Wrnnn Peak_____ 130-PFDoA 615.0/169.0 Da D Baseline Misse n Wrnnn Peak ilM !'WIiii` PFTeA 1 713.0/669.0 Da Baseline u Missed ___ p Wrnnn Peak 13C-PFDoA 615.0/169.0 Da Baseline Missed ___ n W rnnn Peak___ Before After 166 Page 216 of 242 iymbia Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation . 1.38 K1000968-016 X PFOA2 413.0/369.0 Da Baseline Missed u Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed o Wrong Peak PFUnA2 563.0/269.0 Da d Baseline Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da a Baseline Missed a Wrong Peak -.v.i'Y,, ., ' PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After 167 Page 217 of 242 p. 117 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 OVSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-016 2/9/2010 1:18:20 PM PFCkinetexShort.dam 36 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 4903800 6091800 3764000 6091800 6091800 3764000 4894400 4894400 Analyte RT (Exp RT ) 1.27 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 108350 174580 266010 268150 70590 1029900 3764000 4894400 Calc. Cone (ng/mL) 0.15 0.28 0.22 0.36 0.24 0.81 0.87 1.00 Modified? No No No No No No No No 168 Page 197 of 245 CIo! C,J Analytics! Smrsfkm* p. 118 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT ) 1.27(1.27) 3.59(3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.22) 6.31 (6.32) 1.25(1.26) 5.30 (5.30) Area 108350 174580 93747 23145 201380 395370 4903800 6091800 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.35(0.40) YES No 0.09(0.10) YES No 2.85(3.03) YES No 0.38(0.40) YES No 45.26(13.55) No No 22.72(13.06) No 169 Page 198 of 245 Services' Quantitative Peak Review 1.27 L PFBA 1 213.0/169.0 Da u Baseline Missed ___11 Wrnnn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wronn Peak p. 119 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000968-016 PFPeA 1 263.0/219.0 Da Baseline Missed n Wronn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFBS 1 299.0/80.0 Da Baseline a Missed n Wrnnn Poak 180-PFHS 403.0/103.0 Da d Baseline d Missed n Wrnnn Poak J:; PFHxA 1 313.0/269.0 Da Baseline d Missed n Wrnnn Paak 13C-PFHxA 315,0/270.0 Da Baseline Missed n Wrnnn Peak PFHpA 1 363.0/169.0 Da Baseline c Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da Baseline u Missed n Wrong Peak 180-PFHS 403.0/103.0 Da Baseline u Missed n Wrnnn Peak S_180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed ____n Wrnnn Peak___ S_13C-PFDA 515.0/470.0 Da a Baseline Missed ____n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak 170 Page 199 of 245 p. 120 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 1,27 K1000968-016 PFBA2 213.0/169.0 Da c Baseline d Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak *> '.i PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Ifc'nageBrriavalie PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak SJ3C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A o Baseline Missed Wrong Peak Page 200 of 245 171 p. 121 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-017 2/9/2010 2:11:40 AM PFCkinetexLong.dam 37 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 2858500 2285100 5416400 2428300 99693 5416400 114980 114980 114980 Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.38 (2.53) 0.00 (3.34) 4.16 (4.06) 4.14 (4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 0 0 45851 0 6029 8872 0 0 0 Calc. Cone (ng/mL) N/A N/A 0.094 N/A 0.042 0.034 N/A N/A N/A Modified? No No No No No No No No No 172 - i# Page 218 of 242 p. 122 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp R T ) 1.39(1.36) 1.37(1.36) 2.54 (2.44) 2.36 (2.44) 2.40 (2.53) 2.37 (2.53) 0.00 (3.34) 3.36(3.34) 0.00 (4.06) 4.13(4.06) 4.14(4.10) 4.76 (4.64) 4.77 (4.64) 5.29 (5.13) 5.76(5.56) Area 9189 2858500 341 2285100 12550 5416400 0 2428300 0 99693 3010 1410 114980 831 233 Modified? Calc (Exp) MRM Ratio No 0.00(2.38) Ratio Confirms ID No No 0.00(50.17) No No 0.00(0.28) No No 0.00(19.17) No No 0.27(0.32) YES No 118.13(22.53) No No 0.00 (0.15) No No 0.00 (22.10) No No 0.00 (0.22) No No 16.53 (1.86) No No 0.34(0.32) YES No 0.00(0.29) No No 0.00 (3.08) No No 0.00(0.32) No No 0.00 (0.36) No 173 Page 219 of 242 Quantitative Peak Review . . . : / i\ Zj VVu/'./ f ::: '.tKA PFOA 1 413.0/169.0 Da Baseline Missed i--i Wrnnn Peak U S 1 It 13C-PFOA 417.0/372.0 Da c Baseline a Missed i Wrnnn Peak p. 123 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000968-017 I- PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed i Wrnnn Peak : o : j u PFOS 1 499.0/80.0 Da o Baseline Missed tuui ti 4.1h6 q I,,,l A ;l* 1, . 13C-PFOS 503.0/80.0 Da Baseline Missed Wrnnn Peak :iiwo f- ISttfli T _____ L PFUnA 1 563.0/519.0 Da Baseline c Missed __ n Wrnnn PflaK__ !\ 13C-PFUnA 565.0/270.0 Da Baseline a Missed ____n Wrnnn Peak___ .'SOtJC! | 4.77 ju'joo; PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak I To n PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Pnak if../ PFTeA 1 713.0/669.0 Da Baseline Missed i- Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak Before After 174 Page 220 of 242 p. 124 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation K1000968-017 1.39 PFOA2 413.0/369.0 Da Baseline Missed Wrong Peak PFNA "2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS2 499.0/99.0 Da a Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak PFUnA2 563.0/269.0 Da Baseline o Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline a Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline a Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da Baseline o Missed Wrong Peak Before After 175 Page 221 of 242 p. 125 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000968-017 2/9/2010 1:27:51 PM PFCkinetexShort.dam 37 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHxA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 4107400 5157600 3705400 5157600 5157600 3705400 4569300 4569300 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 93304 41237 12849 65460 17139 55713 3705400 4569300 Calc. Cone (ng/mL) 0.16 0.06 0.03 0.06 2 /#0.03 vl/ 0.04 0.92 1.00 Modified? No No No No No No No No 176 -ry-4 fU o Page 201 of 245