Document DvKLR48GBooyLXLYyqyv3y1BM

ILLEGFi| 191 mi .2 110573 R&S 028231 animal*. The major difference lie* in the larger maintenance requirement* of I lie mouse due to its hiilur nnialmlic rate. Philip J.n olivin J110563; Levels of immunoglobulins i-,G.vM.vA) in normal ildren. Taoala, Kiluiclnru (Kyoto I'liiv., Kyoto. Japan). uniko Kly* I9ft8. IHij, 370-Ni (Hilt;). L(u|. l eriim immunoglobulins were tletd. by tlieantibody agar plate luetliod in maternal and cord blood at the time of delivery and in groups of normal infant* and children up to age la. IA was absent from cord blood, >M present only in traces, whereas the levels of yC in cord blood were equal to nr above thir-c in maternal blood. Minimum levels of yC were found at 3 and A months, then rose slowly, not attaining the adult range until ugc 11. Levels of yM reached half the range found in adults'by 3 months, but did not reach adult levels until 3 yrs. Levels of yA were less than half the adult range up to age 5, then rose rapidly. Jacob Sacks 110564k Polymorphism of transferrins of bovine, porcine, and canine aera, as determined by neons of acryUmide-gel disc electropboreaii. Comer, Joseph L. (Univ. of Illinois, L'rbana, III.). 1968,66 pp. (Eng). Avail. Univ. Microfilms. Ann Arbor, Mich., Order No. 69-10.687. From Pits. Abslr. B 1969, 29(12), 4727. SNDC 110563n Homoglobin-Leporo Baltimore, a third type of a 40 crossover (4", 0**). Ostertag, Wolfram; Smith, Ernest WenflcU (Sch. of Med., Johns Hopkins Univ., Baltimore. Md.). fur. J, Biotktm. 1969, 10(2), 371-6 (Eng). A new Lepoee-type Hb jn a person of Negro descent ha* been identified. In this person e minor Hb (Hb-Lcpores.itia) was detected. An abnormal 40-chain was isr* *ed and fingerprinted. This hybrid-type pro tein presumably arose as a consequence of unequal crossing over between the I and 0 genes, as was concluded for Hb-Leporea_iM and Hb-Lcporeg.iuasia- In the new Lepore-type Hb described here the crossover could be placed between residues * and 0". This type of crossover should occur with the highest frequency if crossing over is equally frequent between any nucleotides and if selection favors none of the crossover types. RCBY 110566a Genetic and developmental regulation of hepatic 4-amiaolevulinau dehydratase to mice. Doyle, Darrell J.; Schimke, Robert T. (Sch. of Med., Stanford Univ., Stanford, Califj. J. Biol. Cktm. 1969, 244(20), 6449-59 (Eng). The mechanism by which the levulinate locus (L) regulates the tissue ^ttivity of l-aminolevulinate dehydratase in mice has been examd. He assayablc hepatic enzyme activity in homozygous is* mouse v^utiu, represented by C57B1/6, is between one-third and one- half that of homozygous It* strains. This difference in enzyme activity is due to a difference in amt. of enzyme protein as demon strated by hnmunochem. techniques with an antibody specific for 4-aminolevulinate dehydratase. Combined immnnochem. and isotopic techniques show that the ievuiinate locus regulates the conen. of hepatic l-aminolevulinate dehydratase by acting nt the level of enzyme synthesis. The rate of degradation of hepatic enzyme is the same in both low and high activity strains; when expressed as a half-life, this is equal to 5-6 days. The pattern of 4-ammolevulinate dehydratase development with age is similar in livers of both low and high activity strains. The specific activity of the enzyme is high in fetal liver, decreases during the several days prior to birth, and increases to the adult level during the first 3 weeks of postnatal life. The activity of 4-amino- levulinato dehydratase in fetal liver b also regulated by the levulinate locus. The enzyme in fetal liver is approx.' twice as active catalytically as the enzyme in adult Ever relative to iti function aa an antigen. The fetal enzyme else appears to be different in stability to heat and proteolytic inactivation. How ever, fetal and adult enzymes are similar by other physiochem. criteria, including electrophoretic mobility, sedimentation coeff., and Km for the substrate. RCJN 110567e Identification and genetic control of two now low- density lipoprotein allotypes: phsaogroupn at tha Lpq locus- Albers, John J.; Dray, Sheldon (Med. Center, Univ. of Illinois, Chicago,. III.). J. Immunol. 1969, 103(2), 155-62 (Eng). See CA 69: 104403a. VNJZ 1105$8q Modification by crude histones of gene activity for lactate dehydrogenase. Latner, A. L.; Longstaff, . (Roy. Victoria Infirmary, Newcastle upon Tyne, Engl.), S'aturt (London) 1969, 224(5214), 71-3 (Eng). Addn. of crude calf thymus histone to the mouse kidney organ culture increased the proportion of the lactate dehydrogenase (LDH) isozymes which moved more rapidly toward the anode during electrophoresis. JLn crude rat liver histone fraction was added to the culture, the flstaic was in the direction of the slower moving LDH isozymes, the cultures exposed to the crude call thymus histone, the percent of LDH-1 and the H subunit contribution to the tout LDH activity significantly increased, whereas the opposite was found with the rat liver histone prepn.; LDH-5 and the M sub unit significantly increased. Enrichment of the medium with amino acids present in the caJf thymus hbtone prepn. did not alter the isozyme pattern, indicating that the changes in LDII pattern were not caused by hydrolysis of the histone. Gene masking by histones apparently is nonspecific, and the crude ext. may contain factor*, not m-rc--arily liNtonr*. which affect gene activity in a manner that >. not .m i,.. sjHvitic. 11SJN' HOSoOr Clinnr.c* in the atliti'v >.r isnlit-'d oscular smooth muscle in response to reduced osmel.iiity. Pm -,n. t >l>>f tl'niv. Goteborg. GiitcliurL:, Swed.i. .1,In / .'.</. 1969, 77(1), 191-2(0) fling). 'Hie .:?.( of liy| i.:. !anl> on tin- spon taneous activity ol the miiooiIi muscle m tile isolated portal vein of the rat have been studied in snln*. with varied ionic Cumpn. A comparison between the stimulatory effects of lowered osmolarity and inerca-ed [K*l, (external K' conen.) indicated that at normal |K 'l- in the control state the excitation rauxed liy hypo- tonicity is not mediated only via changes in the |K*|,'|K.*!t ratio. The rcsimiise to hypoosmolariiy at a higher |K*|, is, however, more readily explained by the change in IK'I./IK*]* which occurs in assocu. with the swelling of the cells. This might be attributed to the fact tliat tlie membrane potential oi smooth muscle is more dependent on the transmembrane conen. gradient for K* at higher levels of |K*|. The stimulatory effects of hypo- osmotarity are markedly reduced at a low |.\a*] but are not considerably affected by comparable redns. in |K*j or [C3~],. This indicates that the transmembrane Na* gradient is involved in the mechanisms by which hypoosmolariiy excites the spon taneous activity of vascular smooth muscle. One possible interpretation is that the swelling of the muscle cells in hypotonic solns. causes an increase in the permeability of the cell membrane to the relatively larger Na*. RCVV 110370J Secretion of rat pancreas perfused with plasma from rata fed soybean trypsin Inhibitor. Khsyamhasbi, H.; Lyman, R. L. (Univ. of California, Berkeley, Calif.). Amtr. J. Pkytiol. 1969, 217(3), 646-61 (Eng). When plasma from rats fed soybean trypsin inhibitor was perfused through an isolated, fasted rat pancreas, amylase secretion was 2-3-fold greater than that of a pancreai perfused with plasma from rats fed the same diet without trypsin inhibitor. Addn. of atropine to the perfusate did not inhibit the secretory response. The plasma factor was relatively unstable upon storage at 4* (50% loss of activity in 4 days) and appeared to be assoed. mainly with those plasma proteins of 10,000 mol. wt. or less. Thus, active soybean trypsin inhibitor enhanced the formation and (or) release of a humoral pancrenty- minlike substance that markedly stimulated external enzyme secretion of the rat pancreas. RCDH 110571k Effects of diet and catechol amines on bile add metabolism in dogs. Cans, Joseph H.; Cats, Marilyn R. (Coll, of Med., Univ. of Vermont, Burlington, Vt-). Amur. J. Pkytiol. 1964, 217(4). 1018-24 (Eng). Five adult male dogs were surgically prepd. with polyvinyl tubing placed in the upper duodenum. Each dog was given chotic-car5myf-1'C acid and Samples of gallbladder bile were withdrawn over n 10-day period. 'Hie taurocholic acid pool was 1-00 g./lO kg. body art. and half- life was 3.2 days when dogs were given n dry dog food dirt. Epinephrine In oil, 0.8-1 mg./kg./day, increased plasma cho lesterol concns., produced n 23.0% redn. In the taurocholic add pool site, and increased the rate of decline of specific radioactivity of both taurocholic and taurodeoxycholic acids. The half-life of taarocholic-uC acid during epinephrine administration was Iff days. Norepinephrine in oil, 0.8-1 j0 mg. free base/kg./day, produced a modest decrease in the half-life of taurocholic-S'Caod without significant elevations in plasma cholesterol eoocas. A dietary supplement of */ lb. beef mu* 2-3 g. of cholesterol per day resulted in increased plums cholesterol concns., increases in n taurocholic acid poo) size, and a decrease in the half-life of taurocholtc-t^C acid. Epinephrine administration further m- creased plasma cholesterol concns. in cholesterotted dogs, hut did not significantly alter bile acid metabolism. RCDH 110572m Effect of osmolarity on canine renal vssruier resistance. Gaxitua, Sergio; Scott, Jerry Benjamin; Chan, Ching-Chuag; Haddy, Francis J. (Michigan Stale Univ., East Lansing, Midi.). Amor. J. Pkytiol. 1964, 217(4). 1216-23 (Eng), The effects of changes in renal blood osmolarity, pro duced by close intraarterial infusion of solns. of dextrose, NgCJ, and urea, on renal resistance were systematically examd. in the dog. , Infusion o( a hyperosmotic snln. of dextrose for 3 min. produced a fall in renal vascular resistance which was sustained for the entire infusion period. On termination of the infusion, resistance returned to the control level. Infusion of a solo, of hyperosmotic NaCl or urea, on the other hand, produced a fall in resistance which waned with time. Furthermore, on stopping the infusion, resistance transiently rose writ above the control level, particularly in the case of urea. Intraarterial infusion of an isosmotic snln. of urea and of hypnsimitic solas, of all three substances produced large increases in resistance. Studies con cerning lymphatic vessel pressure and cell-free perfusion, as well as the findings of others, are consistent with tlie hypothesis that the changes in resistance result to a large extent, from active vasninotion, possibly due tu osmotic changes in the water content of the smooth muscle cells. RCDH 110573a Metabolism of atlyl compounds in tbs nt. Clapp, J. J.; Kaye, C. M.; Young, Leslie (Si. Thomas's liosp. Med. Sell., London, Kngl,). Biorkrm. J. 1964, 114(1), fir-71* (Eng). A mercapturic acid was isolated from the urine of rats treated sc. 110574 > Chemical Abstracts V<X. 71. 1969 * 142 with CH:CIIOAe or CII:CIIOII and identified as 3-hydroxy- studied. A dose of 2(00 I.U. L-asparaginase/kg. evoked hyper propylmcrcapturic acid (lV-acctyl*.S'(.'l-liydrxypropyl)-i.-cy*- glycemia and glycosuria in most ol the animals; this is equal to a tcinc, 1). There was no evidence fur the presence of allyltner- dose which induced hyperglycemia when given to patimls. All caputric acid (.Y-*celyI--V-*llyl-L-cystcinet 11) in the urine of rabbits receiving 10,900 I.U. L-asparaginasc/kg. or more showed these animals. II. however, was detected in the urine of rati diabetogenic responses, but these effects were temporary (2-13 injected t-c. with Cl(;ClIC1. .V-allylglutathione, and .V-allyl-t.- days) and were not assoed. with any histological changes in the cysteine. Rats treated with CH,:CHC1 also excreted 1. Mer- pancreatic islets, except in 1 rabbit which showed islet hyalinixa- capturic acids apparently arise hy (lie reactiun of ally! compds. or tion. DDJX their active dcrivs. with glutathione. BQJN llOSSlp Effect of Bidrin on the metabolism of dietary com l)0574p Metabolites of octoclothepina eliminated in human ponents by the bovina. Lowrey, R. S.; Bowman. Malcolm C.; and rat urine. Queisnerova, Milena: Svatck. E.; Metysova, Knox, F. E. (Coastal Plain Exp. Sta., Agr. Res. Serv., Tilton, Jirina (Res. Inst. I'liarm. Uiochein., Prague. Czech.). Biocken. Ga.). J. Dairy Set. 1969, 52(9). 1460-3 (Eng). Si* steers J. 1909,114(2), 339--12 (Eng). Four metabolites and unchanged averaging 400 kg. in wt. were used in a switchback trial to study octoclothepine were extd. with dichloroethane from the urine of the effect of spiking corn sibge with 15.7 ppm. (0.24 mg./kg. bin,tans given octoclothepine. These substance* were isolated body wt.) of the pesticide Bidrin (3-(dimethoxyphosphiByloxy)- and purified by column nnd thin-layer chromatog. By chro Al,lv-dimethyl^rr-crotonxmide| on blood cholinesterase, digest matographic, spcctrophotonietric, and polarorraphic anal., ibility of dietary components, utilization of X, and rumen fermen unchanged octoclothepine and 3 of the metabolites were identified tation. Bidrin residues were not found either in the urine.or in (noroctoclothepinr, noru.todolhepinc .V-oxide, and octoclothc- the feces of animals ingesting Bidrin. Azodrin. a metabolite of pinc 5-oxide). The presence of glucuronides in human urine Bidrin, was not present in the feces of treated animals but aver was shown. The same metabolites and unchanged octoclothepine aged 0.430 ppm. in the urine. Based on urine vol. this repre were also found in rat urine by chromatog. RCFC sented 1.6% of the ingested Bidrin. Blood cholinesterase was 110575a Mechanism by which leucine and arginina stimulate depressed to 30% of pretreatment values by the end of the 42-day DO release la vitro. Milner, R. D. C. (Univ. West Indies, exptl. period, whereas activity of steers fed untrezted sibge was Kingston, Jamaica). Biockim. Biopkyt. Ada >909, 102(1), unaffected. In the metabolism trial conducted during the last C/> J54-45 (Eng). Basal insulin release in young rabbit pancreas 14 days of the 42-day period Bidrin bad no effect on the digest slices in vitro was higher in the presence of leucine (SraA/) than ibility of dietary components (dry matter, oxide protein, ether o IO 00 IO CroO tts; of arginine (5mA/) (in the absence of glucose). Glucagon (5 pg./ml.), theophylline (1mA/), dibutyryl cyclic AMP (lmif), or L-argininc (5mJ/) stimulated insulin release in the presence of leucine but not in the presence of arginine. Arginine-stimulated basal insulin release perhaps works by releasing glucagon from the w-cells- The mechanism of the leucine-induced stimulation ext., crude fiber, N-frte ext., and gross energy), the vtSatioa of N. or rumen fermentation. KCLD 110582a Effect of a second doe* of eadminm aalti on vascular permeability in the rat trade. Clegg, B. J.; Carr, Ian; Niemi, M. (Univ. Sheffield, Sheffield, Engl-)- J. Endocrinol. 1969, 45 (2), 265-8 (Eng). Two doe** of CdCh were given to rats et is different, probably via the adenyl cyclase system. BSJN interval* varying from 78 to 194 days. Afto the second ooae the 110570r Cytochemieal study of the distribution and content of blood vessels hi the testis and initial segment of the epididymis nudeoproteia* end of toms functional groups of proteins in iso showed Increased permeability, as demonstrated by i.v. markers. lated formations of the central nervous system following foreign In the testis, IcrJchtg vessels were usually found fat areas where protein injection. Tumanyan, B. L. (Erevan. Med. Inst., interstitial cells were abundant. RCKV Erevan, USSR). Biol. Zk. Ann. 1969, 22(5). 97-8 (Russ). llOS&lr Add-base and electrolyte changes Induced by acute Cytoplasm of various parts of rabbit central nervous system isotonic saline Infusion in the nephrectomizod dog. Rosenbaum. (including intervertebral ganglia, nuclei of the XII cranial nerve, Barry J.; Makoff, Dwight L.i Maxwell, Morton H.; Hogb- and skin motor area of brain cortex) displayed higher ribonucleo- ought, Michael (Ccden-Sinai Med. Res. Inst., Los Angeles. protein content in animals treated with repeated i.v. injection of Calif.). J. Lab. CUn. Iftd. 1969, 74(3). 427-35 (Eng). The normal horse serum. The peek effect was observed on the 4th mechanism and extent ef "dilution acidosis** by acute uriusioo* day after the fast injection. K > changes were seen in content of isotonic saline we* studied in nephrectomizrd dogs. Iufusioas end distribution of protein SH groups. If. Jasincruk of 50,75, and 100 ml./kg. of body wt. (BW) (Groups 1,1. and J) 110577s DlfA synthesis in moose embryonic fibroblaxt-Uke over a 30-min. period resulted in extraedfubr fluid expansions of cells in ritro, stimulated by various factors. Vasil'ev, Yu. M.; 34.4 1.7%, 23* dfc 2.2%, and 33.9 5J%, rerp. pCO, was Gel'fand, I. M.: Gershtein. V. I.; Fetisova, E. K, (Most. Gos. kept const. Body spaces were measured isotopicaby, and intra- Univ., Moscow. USSR). Dokl. Akad. Nook SSSR 1969,187(4), cellular H* activity by 5,5-dimcthy]-2t4-oxazolidwedione-MC. 9I3-I5[Cytol| (Russ). Autoradiography was used to follow the The extracellular bicarbonate concn. decreased only --2.4 0.7 DNA synthesis in single-layer cultures of mouse embryo fibro mcq-/I* and --2* 0.4 meq./l. in Group* 1 and 2 and --3JO ' blast-like cells in lactaibnmin hydrolysate and ox serum media in 1.0 in Group J. The total amt. of extracdfalar bicarbonate in {he presence of thymidine-1// added at various periods of culture creased +0A 02 meq./kg. of BW, +03 0.2 meq./l. of age. The index of *H inclusion was increased by addo. of tes BW, and +1.5 0.4 meq./kg. of BW in each group, rerp. Con ticular or bacterial hyaluronidases, RNase, and digitontn. The comitantly, the total amt. of extracellular field K mcraased +0J rise of *H intake was the result of stimulation of the entry of the 03) meq./kg. of BW, +0.5 0.1 meq./kg. of BW. and +0.4 cells into the 3-phase of development. C. M. Kosobpoff 0-1 meq./kg. of BW, reap-, while there was no decnase in 110S78t Effect of pregnancy and oral contraceptives on gin- extracellular K ccmcn. It was suggested that the extraceSnlar bi cost and insulin metabolism. Taskinen, Marja K. Dnodecim carbonate end K concur, were defended by shifts of H* into ceps 1969, 85(11), 670-83 (Furnish). A review. The effect of both (or bicarbonate out of cells). In conjunction with n ddt of K pregnancy and oral contraceptives is dtabetogenous, and they out of cells. Despite the presumed H* (or bicarbonate) Dor, may cause deterioration of glucose tolerance in healthy women. there were no net changes of H* activity. The conceptual The organism tends to compensate this far enhanced insulin theory of extracellular "dilution acidosis'* it valid but cf Emited secretion, which leads to hyperinxulinism. 43 references. magnitude and little din. significance when expansion is relatively I. G. Zri slow. Cellular buffering minimixes the extracellular acidosis, 110579a Effect ef pyridoxal 5'-phoephate on the strength of and the shift of K out of cells prevent* hypokalemia. RCKY skin In vitro. Harkscss, Robert D. (Univ. Coll. London, Lot - 110534* Effect of erythropoietin on ben* marrow eminn- don, Engl.). Ezptriralio 1969, 35(10), 1048-9 (Eng). Treat Icvul'aic add xyuihetaao and bem* synthetase. Bottomfey. ment with 10mA/ pyridoxal S'-phosphate (I) ia vitro reduced the Sylvia S-; Smithee, G. Ann (Veterans Admin. Hosp-. Oklaboma strength of rat tail skin progressively with time of incubation. City, Okie.). J. Lab. aim. Mod. 1969. 74(3), 445-52 (Eng) The redn. was reversible, with strength returning when the skin An in vitro culture method lies been adapted for rabbit bone ring* were placed in buffered saline. .Pyridnxamine or pyri- marrow end has permitted measurement of the effect of erythro doxine at 10ml/ had r.o comparable effect. The effect of I was poietin on A-sminotevulinic acid synthetase and heme symhetasc reduced in the presence of lysine. Pretreatmeut of the skin with activities in'marrow cells. In this system, erythropoietin en KaBH, followed by washing in'saline buffer, largely inhibited hanced A-aminofcvulinic acid synthetase activity. This effect the effect ol I. rretreatment with NHjOH iNo inhibited the was detectable ms early as 6 hrs. after contact with erythropoiet in. effect of I. Treatment with NH.-OH after I prevented the re The max. cnxyme activity observed was 20GrI greater a stim covery of strength on subsequent transfer >- ihe saline buffer; ulated than in control culture*. Studies with inhibitor* of pro HiXNHi had a similar effect. The effect nf 1 -.i skin strength ia tein synthesis suggested that erythropoietin increased U* syn apparently brought about by rupture of a.- .ncihine linkages thesis of the enzyme. No stimulatory effect by erythropoiet:r foravd between an aldehyde and an amino gr. ip. BSJN cn heme synthetase activity was observed. RCKV ll'ISSOn Diabetogenic effect of i.-ispc zginase Khan, llOSASt Action of adenosine triphcsphxte on the deyre'.ze1. AmanuIUh; Ariaehi. Mitsuo. Hill, Joseph M n-Gln-han (Wad- spontaneous electrical activity of the dog cerebral cortex. 11. nr ley Inst, ol Mol. Med., Dallas, Tex.). J. Clin. F.'tilotrir.al. Gian: Arriguni, Elena; Ferrara, A.; Ala.cHcrpa, I*. Uriah, 1960, 29(10). 1373-0 (Eng). The rffrrts of Eirherirkin Fhannacol., Univ. Pavia. Pavia, Italy). J. Hhnrm. .Sr,. n,*it tali t.-asparagiiuM- <*100-50,01)0 l.U./fc,;.. i.v.), which has ,-cn Sfi(9). 111)1-3 (Eng). The repeated suppression <4 tin vennV used l*i induce ri'ini'.-iim of human acnl*- 'emphatic anil n.yrlug- thiii and the circulation ol the brain in lm- 6c intlii. e* a high* enous leukemias via depletion i*f an ainitv ami essential fur some ptmirat or a silence of elec, activity nf the cortical aad sui neoplastic cells, on rabbit bloiid and urinary glucose levels were cortical centers. The recovery of inpititiry and circulator..