Document Dv7GZKQjwORYO2785e1e7mvba
AR226-3196
TRADE SECRET
Study Title H-25134: Static, Acute, 48-Hour Screening Test to Daphnia
magna Laboratory Project ED: DuPont-8862
AUTHOR: Laurie D. Bouchelle STUDY COMPLETED ON: November 30, 2001
PERFORMING LABORATORY:
E.I. du Pont de Nemours and Company Haskell Laboratory for Health and Environmental Sciences
Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050
Company Sanitized. Does not contain TSCACBJ
H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna
DuPont-8862
CERTIFICATION
We, the undersigned, declare that this report provides an accurate evaluation of data obtained
from this study.
Work Completed by: Issued by Study Director::
AfrLLULJUEL^gUXjuLjM^
Lauri e TD^. Bouch1e1lle
uI^J!^/ Associate Scientist
Alan Samel, M.S. Research Ecotoxicologist
.T>^&wjr)d^^001
Date
2o ^ve^lairZoal
Date
Company Sanitized. Does not contain TSCA CS<
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H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna
STUDY INFORMATION
DuPont-8862
Submitter's Notebook Number(s):
Haskell Number: 25134
CAS Registry Number: ^BB
Composition: UJ|
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Physical Characteristics: Dark brown solid
Sponsor:
E.I. du Pont de Nemours and Company Wilmington, Delaware 19898
U.S.A.
In-Life Initiated/Completed: November 27, 2001 /November 29, 2001
Company Sanitized. Does not contain TSCA C6/
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H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna
DuPont-8862
1) <.
SUMMARY
The acute toxicity of H-25134 to the water flea, Daphnia magna (less than 24 hours old) was
determined in an unaerated, 48-hour, static test.
The study was conducted with 4 concentrations of H-25134 and a dilution water control at a mean temperature of20.3C (range of20.2-20.6C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control was clear and colorless at test start. The 1 and 10 mg/L test concentrations were clear and colorless with undissolved test material on the water surface at test start. The 100 and 1000 mg/L test concentrations were slightly cloudy with undissolved test material on the water surface and on the bottom of the test chambers at test start. All water quality parameters were
within acceptable limits during the exposure.
Exposure of H-25134 to the water control and nominal H-25134 concentrations of 1, 10, 100, and 1000 mg/L resulted in 0, 0, 0, 10, and 10% immobility, respectively, at the end of 48 hours. No immobility or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no immobility at test end was 10 mg/L.
The results are summarized as follows:
Nominal concentrations of H-25134, mg/L 48-hour ECso for H-25134, based on
nominal concentrations, mg/L
dilution water control, 1, 10, 100, and 1000
>1000
H-25134 exhibited low concern for aquatic hazard" in an unaerated, 48-hour, static, acute test using the water flea, Daphnia magna (less than 24 hours old).
1^
W
8 Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation of data. Environmental Toxicology and Risk Assessment, ASTMSTP 1179.
(W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials,
Philadelphia.
Company Sanitized. Does not contain TSCA C81
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H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna
DuPont-8862
TABLE 1
DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES
Nominal H-25134 Concentration (mg/L)
Test Initiation (0 hours)
Dissolved
Oxygen
(mg/L)
pH
Test Completion (48 hours)
Dissolved
Oxygen
(mg/L)
PH
Water Control
8.6
7.9
8.7
7.9
1.0
8.2
7.7
8.6
7.8
10
8.1
7.7
8.6
7.8
100
8.1
7.6
8.6
7.8
1000
7.9
7.6
8.5
7.8
TABLE 2
IMMOBILITY OF Daphnia magna, IN AN UNAERATED, STATIC, ACUTE, 48-HOUR TEST WITH H-25134
Nominal H-25134 Concentration (mg/L)
Water Control 1.0
10 100 1000
Number of Dead Test Organisms /
Number of Test Organisms at Study Start
24 Hours
48 Hours
0/10 0/10 0/10 0/10 0/10
0/10 0/10 0/10 1/10 1/10
Sanitized, Does "o> contain TSCACBI Compan,
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