Document DdmzDR52pVRK9JGEeGgn4kd1o
AR 226 -- OLR]
ACUTE TOXICITY TO FISH (BLUEGILL)
IESTSUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also
be referred to as PFOS, FC-95, or as a componenotf FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3)
Remarks: The 3M production lot number was not noted. The test sample is FC-600. Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80%
starch, and 0.05 % benzotriazole.
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The following summary applies to a mixture with incompletely
characterized concentration of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test
sample.
memwoo:
Method: Not noted.
Test type: Static acute
GLP: No
Year Completed: 1975
`Species: Lepomis macrochirus Supplier: Not noted. Analytical monitoring: Temperature, pH, DO Exposure period: 96-hours
Statistical methods: Probit analysis. Test fish age: Juveniles
Average Length and weight: Length = 1 inch
Weight = 0.5 grams
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Loading: Not noted.
Pretreatment: Not noted.
Test conditions: Dilution water: Carbon-filtered water, City of St. Paul, MN Dilution water chemistry: Not noted.
Lighting: Not noted
Stock and test solution preparation: Test solutions were created by
_
direct weights additions.
Concentrations dosing rate: Once
Stabilityof the test chemical solution: Not noted
Exposure vessels: Not noted.
Number of replicates: One
Numberoffish per replicate: 20
001998
Number of concentrations: 5 plus a blank control
Water chemistry during the study:
pH range (0 - 96 hours):
7.1-7.2 (control exposures)
7.27.3 (2,000 mg/L exposure)*
Dissol5ve.d67O.xy0gmegnlLra(ncognetr(o0l-e9xp6oshuoruerss)):
4556.0 mg/L (2,000 mg/L exposure)*
Temperature (0-96 hours):
*
72F Values for
the
2,000
mg/L
exposure
(third
highest
concentration)
were used because total mortality occurred in the highest
concentrations tested.
RESULTS:
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Nominal concentrations: Blank control, 1,000, 1,500, 2,000, 3,000, and
4,000 mglL Element value:
96-hour LCso = 1,500 (1,282 -- 1,755) mg/L.
Element value based on nominal concentrations.
RSuebmsatraknsc:eTReesmtianrgkwsafsielcdo. nTduhcetveadlouen trehpeomritxetduarpeplaisedsetsoctrhiabtedmiixnttuhree Taensdt not the fluorochermical proportion alone.
CONCLUSIONS
T1h,5e00FCm-g6/0L0w9i6t-hhaou9r5L%CcsonffoirdLeenpcoemiinstemrvaaclroofch1i,r2u8s2
was determined to 1,755 mg/L.
to
be
`Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
pataQuaLTY
Reliability: Kiimisch ranking 3. The methodology. The sample purity was
study lacks documentation not properly characterized
on and
the
study lacks analytical confirmation of the amount of fluorochemical
proportion in the solutions.
REFERENCES
---- #211
This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, 1975.
OLTasHtEcRhanged: 6/28/00
071299
* . 7a0101 .
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITYWORKSHEET
--E-- E --------
DeTes 96-Hour Static
Test Organism, Bliegil
Date Started,
os
Time Started
2:00 Pre
Material Tested fe-coo
Avg. Weight a5 size_{. Tuk,
Date Completed,
or
Plotted gg
Dutton serCoton Gilteted city afer
Lepomis Haclochitus
24 hrs
48 hrs
72 hrs
.
96 hrs
Conc. survival pi pm survive a survival bra Survival pit pre
cma] og[7 70] 100[226s] loo [ral6o[100Jzi[5d
cosal (00 rels] 10 16a] 9p Tro[ss|golzaled
0oV iow Ja 6a too freleq| 70 [n:[sa]50Tada
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400L0 ofelad 0 [E10THT 0IF]
forage Temrry 72] 70
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ENVIRONMENTAL ENGINEERING LABORATORY
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AQUATIC TOXICITY - PROBIT ANALYSIS NORK SHEET ST COPY AVAILAB
Date Started: -3-7,
Material Tested:
-
Tine Started: 2:00 (4M)(PM) Test Organism por
Bly egil <
Type Test:_(ContRiitup)(uStoatuice) Avg. Wt. (gram) Avg. Size_ 1) J (inch)
Exposure Period:
96--tour pate Completed:
-29-1F
Diluent:(carbon-File,-St.P. City Wer.) Analysis by: WT. Zug) hp
[] x
@
o-& Contributior
No.PlootfteDdoses |Conc.] OMbosretravleidty%|| MEoxrpteacltietdy || CorVraelcuteesd| EObxspeercvteedd-|| Ntoocmohgir(afphro#m1}
1 head 20| /3TT 7 1 048|
2 lsooll 50 | 50 | | -- TT-- |
5 loss Zo| 78
TT 2 1 037 |
5
11 TT 1 1
6
rrrr 1
7
rr [ 1]
k=__ 4 Aimals/oses 0 Total contribution to chi = _2, 980
Total Animals = RB0 _(ehi?)= contrib. to chi x Tot. Animals =_ Abo
ni? -.05) for (x0)
3 degree of freedom (fromTable 2) = _ 5.90
Ab is less than __509 ; therefore, the data are not significantly
heterogenous.
9) Confidence Linits for LCgp*
LCgy =
USO pf Lg =
Lsconyl) Wg =
[o50 wl
Slope function (S) = Ig,/LCs, + LCgqo/LC;q = ASC es sco /0 AT AA =
Az
Total mbes of anissls used between 16} and 84% expected effects OV)= 4o
ficgy =
L(V) "= _(gronNomograph 2
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LCgo/=LlCogweqr limit =\Boo/\\=1
Ei
!
Legg X fLCg, = upper limit = \SooX\\1 =
Logo =
\Soeo
(95% confidence limits Z
to \155)
(1) cDoonnsoetcutliivset m1o0r0e8 etfhfaencttsw.o consecutive 0% effects or more than two
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(2) Expected value for any dose should be greater than 0.01% or less than 99.99%.
(3) Corrected value for each 0% or 100% effect (from Table 1).
* There is a 95% chance that the true value of LC, lies within these limits.
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