Document DMrz9RN38zB8N023gyxVxxDEo

Younger Laboratories BiacJt&KtiU .. . PUaimacoiogUiL .. . AnalfUi 12S CLIFF CAVE ROAtf Saint Louis. Mo.. 63139 PBONBl TlLDBN 6-3849 SUBJECT Toxicological Investigation Of: MC S Monsanto Sample Number 168 Monsanto Project Number Y-66-163 15 3 November 9th, 1966 STUDY CONDUCTED FOB - Monsanto Company, St. Louis, Missouri EXPERIMENTAL PE0CE5UE - A) Oral LDjQ (Bats, Mixed Sex) The undiluted compound was fed by stomach tube to Spr&gue-Dawley strain albino male and female rats. After the approximate Minimum Lethal Dose mas determined, groups ef male and female rats eere fed in increasing doses at increments of 0.1 fractional log intervals at four levels designed to blanket the toxicity range thereby supplying data for calculatien of the LDS0 ehich aaa done according to a modification ef the method of E. J do Beer. Observations were made for texie symptoms and the viscera of the anion Is that succumbed were examined maeroacopically. The data are shown in Table I. B) Skin Absorption MLD (Babbits, Mixed Sex) The undiluted compound was applied in increasing doses ad.increments ef 0.2 fractional log intervals te the closely clipped, intact skin ef New Zealand white male and famale rabbits. ' .. The treated areas were eevered with plastic strips snd the animals placed in wooden stocks far periods up to twenty-four hours, after which tine they were assigned to individual eages. . Observations were made fer toxic symptoms and the viscera of the animals that succumbed were examined macroscopically. '' The data are shows in Table II MET 005703 WATER PCB-SD0000047103 To: Monsanto Company St. Louis, Missouri Younger Laboratories Certificate of Analysis - Page 2 (11/,V c v_. io3 SUMMARY - H c s_i53 A) Oral LD,0 (Rats, Mix&d Sex) The Oral LD50 for sale and female rats mas placed at 7 050 milligrams per kilogram with loser and upper limits of 6,005 to 10,130 milligrams per kilogram. The compound ms classed as practically non-toxic by oral ingestion In male and female rats. B) Skin Absorption KLD (Babbits, Mixed Sex) The Minimum Lethal Dose by Skin Absorption in male and female rabbits ms found to be greater than 1260 milligrams per kilogram and lesa than 2000 milligrams per kilogram. The compound vas classed as mildly toxic by akin absorption in male and female rabbits. YOUNGER LABORATORIES BY: FRH> M. YOUNGER Tb# material in this report is to be used in development of the product and may be given to responsible sales contacts, but it im not te be used by them in advertising copy. The genres of this material is not to be divulged until it appears in formal publications. No exoeptions to the established rule may be made without the approval of the Medical Depart ment in St. Louis. Customers' inquiries regarding matters of toxicity are te be referred as before to the Medical Depart ment in St. Louis for reply. -- Monsanto Company MET 005704 004954 WATER PCB-SD0000047104 To! Monsanto Company St. Louis, Missouri Younger Laboratories Certificate of Analysis - Page 3 (li/y/bo) Y-6c-16`) TABLE I THE OBAL LD90 OF 'MCS 153' FOB BATS Saaplo Fad Undiluted AniaalmNo.i_i_gax eight Dose Fate , 1- Feaalo 2~ Halo 3" Feaalo 4- Male 5- Female 6- Male 7- Feaalo 8- Male 9- Female 10- Male n- Faaale 12- Male 13- Faaale 14- Male 15- Female 16- Male 17- Faaale IB- Male 19- Female 20- Male 230 245 215 260 220 250 22 5 260 230 255 215 270 235 250 230 260 225 245 215 250 5,010 5,010 5,010 5,010 5,010 6,310 6,310 6,310 6,310 6,310 7,940 7,940 7,940 7,940 7,940 10,000 10,000 10,000 10,000 10,000 survived Survived Died Survived Survived Survived Survived Survived Died Survived Survived Died Died Survived Survived Died Died Died Died Survived DISCUSSION - The Oral LD90 for salt and faaale rats *as placed at 7, B50 milligrams per kilogram ' with lover and upper limits of 6,085 to 10,130 milligrams per kilogram. The compound earn classed as practically non-toxic by oral ingestion in male and female rat*. Survival time ms several hours to four days. Toxic symptoms included sovere tremors, lacriaction, salivation, loss of appetite, and paralysis. At autopsy thoro were hemorrhagic area a in the lungs as well as liver discoloration. MET 005705 00-1955 WATER PCB-SD0000047105 ToJ Monsanto Company S:. Louis, Missouri Younger Laboratories Certificate of Analysis - Page 4 (11/5/6C, - Y-=o-l-o TABLE II THE MINIMUM LETHAL DOSE OF 'MC S 153' BY SKIN ABSORPTION IN RABBITS Sample Applied Undiluted Animal _No._">>|ex Weight -SJLa. Dose Weight Change 5 Bay s Later Sfc___________ Fate 1 - Female 2.6 794 2 - Male 3.0 126o 3 - Female 2.5 2000 4 Male 2.8 3160 5 - Fmale 2.5 5010 6 - Male 2.7 7940 0.0 - 0.2 - 0.2 - 0.3 - 0.3 - 0.4 Survived Survived Died -- 7 Days Died - 12 Days Died -- 5 Bays Died -- 7 Days DISCUSSION - The Minimum Lethal Dose by Skin Absorption in sale and female rabbits eas found to be greater than 1260 milligrams per kilogram and less than 2000 milligrams per kilogram. The compound vas classed as mildly texic by skin absorption in sale and female rabbits. Survival time ms five to twelve days. Toxie ayaptarns included reduead activity and appetite, increasing weakness, collapse, and coaa of six te twenty four hours. At autopsy there ms pulmonary and renal hyperemia. MET 005706 001956 WATER PCB-SD0000047106