Document DMMRe9LVjONwanwDjRxBQjGyM

AR226-2867 Copies E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT NO. 56-78 MR NO. Material Tested__________ Haskell No. Sample Ready for Testing Other Codes Material Submitted by 48-HOUR LC502 TO DAPHNIA MAGNA Procedure: The test material, in original form, was diluted with test water to yield the desired concentrations in a 400 ml final volume. After mixing, each concentration was split into two equal volumes and introduced intq separate 250 ml glass vessels. Identical jars containing only test water were designated as controls. A nominal total of 10 daphnids (Daphnia magna; less than 24 hours old) was placed Into each vessel. Mortality counts and observations were made at 1, 3, 24 and 48 hours after initiating exposure. No aeration or food was provided during the test, and temperature was maintained at 18 0.2C. Dissolved oxygen, pH and conductivity measurements were taken on the control and high level exposure solutions prior to test initiation. Calculation of the 48-hour LC50 and confidence limits was by probit analysis (Finney, 1971).3 RESULTS : Definitive Test Test Concentration (%) 24 21 18 15.5 10.0 7.5 >ntrol Initial Water Quality Observed Mortality (%) Supply 24% Replicate A Replicate B ______________ Water Exposure 100 100 40 10 0 0 0 100 100 50 10 0 0 0 Dissolved Oxygen (ppm): pH: Conductivity (pmhos): 8.6 7.4 370 8.7 9.0 400 ______________ Observations__________________ No distinct sublethal effects were observed. 48-hour LC50: 17.8% 95% Confidence Limits: 17.1% and 18.6% Dose-Response Slope: 25.6 95% Confidence Limits: 15.6 and 35.6 Comp a n y Sanitized. Does not contain T S C A CBi RESULTS Con* t. - ., . nH of the 24% concentration approaches values which may potentially cause or contribute to an observed toxicoloeical^response. Similar observations on bluegill sunfish bioassays prompted supplementary tests with neutralized toxicological P .i Those studies indicate that the observed pH did not contribute to the oE \ Z \ Z l m t e r l W H n 3 u e 8ill blo.ssey and susse.t thet the observed pH did dot eppt.cl.bly cocttlb- ute to the acute toxicity of the test material in the daphnid bioassoy. _________________ ______________________________ 18.6%. very low acute toxicity to Daphnia magna under static Its *8-hoar 1C50 Is 17 8% Itb 95% eocEidecce ll.lts of 17.1% end The slope of the dose-response curve is 25.6 with 95/. confidence limits o . Composition o 2 LC50 = The concentration which is lethal to 50% of a population of test organisms during the specified time period. 3 Finney, D. J., Probit Analysis, 3rd ed., 1971. Cambridge University Press. Date Issued: 2/10/7b Report Ko. 56-78 Report by: KaflSy C. Goodman, Technician Approved b y :__ V __( o Q i i u ^ L ^ hillip V. Schneider^ Jr. Ihief, Aquatic Toxicology - 2- Company Sanitized. Does noJ contain TSCA CB! Iac lo te : JCaLi. 0i; uUl. It-i tHiMi1Jj.^- i V TO LGu( 10) Iil U'iTA lOj 2.GC00 2'1.0000 21.0C0C 21.0000 lE.0000 1 8 .COCO 15.5000 15.5000 10.0000 13.0000 7.SOCO 7.5000 LGC LOSE 1.3302 1.3302 1.3222 1.3222 1.2553 1.2553 1.1903 1.1903 1.0000 1.0000 0.3751 0 .S 751 t j/PSLi 1100.. 10. 10. .10. 10 1C. 10. 10. 11C0.. 10. h RESPONDING .11 00 . 10. 10. n. 5. 1. 1. c. 0. 0. 0. K/.Tt 0.9995 0.9999 0.9999 0.9999 0.900C0. 5OC0 0 . 100C C.. 1.0.00 C.OCC1 C.OCC1 o.ccoi C.0C01 profit f.7191 0. 7191 .7191 E.71C1 H.99-7 3.7131 3.7131 1.2.-05 1.2C5 1. ?v*'5 C'Ji.ii'uLi SANFLE Ci.iiiOU RKSPUi.*';* hI E sO.ocooo iPi.jA.<LJU3 HL^POh o w h*L heterogeneity factor UiUl- 01- rOX.*^ DEGREES OF FHEECO.i CEVIATE G JEER OF CVCLF3 AVG X AVG y AVG V SLOPE intercept CHI SQUARED 1.0000 u 10 1 .9000 i. 1531 . 1.2510 .0135 !.<jin9 25.6200 . 27.0335 2.1339 LL = 15.5951 L = 33. 6*165 \ (I I " uF THE liAXIHlJl* LIELlllOl1 EIl.E POR ,-113.0 . *'LM1 * * Company Sanitized. Does not contain TSCA CBI .. ' u f IH u x ir .u t . t i t U i :0W UI. IlH . L S T H 'A T p A!- ! SLUFt's cr).C2Ci _ ; 1i.Tl.t>C!'.f <- "2nt7-i.l'0U^'i5-sU r!wr,-l'a--np.n0oOOOOO 7.0 6.9 o*c 0.7 6.6 0.5 6.4 0.3 0.2 0.1 0.0 5.7 5.6 5.5 5.^ F R 5.1 0 5.0 b 4.9 I 4.0 T 4.7 4.0 4.5 4.4 4.3 4.2 4.1 4.0 3.0 3.E 3.7 3-0 3-5 3.4 3.3 3.2 3.1 3-0 tul biF ITXECTIii Cr. IL L : 6. C i GLhFj ED T I. LUIT , 0:0.55 + * + + 4 + 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 Company Sanitized. Does not contain TSCA CP"