Document DM2EGpapwpeg0LM3DovJmeeko
:
AR226-0102,
DIETARY ACUTE MALLARD DUCK STUDY
EE-II
TEST SUBSTANCE
Identity:
Perfluorooctanesulionate; FC-95. (1-Octanesulfonic
may acid,
a1l,s1o,2b.e2,r3e,f3e,r4r,e4d,5t,o5.a6s,6P,7F,O7,S8,o8r,6-
heptadecafluoro-, potassium salt, CAS # 2795-39-3)
fRreommar3kMs:lot
nuTmhbeetres2t17s.ubsStaamnpcleeiswaaswhsittoerepdowudnedre.r
Sample ambient
was taken conditions
NprMioRr taontdesetilnegm.enPutrailtyandaeltyesremsinteedchtnoiqbuees9.0.49% by LC/MS, 'H-HMR, "F-
METHOD
Method: OPPTS 850.2200, OECD 205, and FIFRA Subdivision E. Section 7T1y-p2e: Dietary acute YGeLaPr: cYoempsleted: 2000
Species: Anas platyrhynchos
SAnuaplpyltiiecra:lWmhiosntiltionrginWgi:ngPs,FOInSc.,meHaasnouvreerd,oInL Day 0 for homogeneity in feed and verification, and Day 5 for stabilty. Test phases: Acclimation - days
EPoxspto-seuxrpeos-u5redaoybs.servation - 3 or 17 days
Statistical methods: LCs values calculated by probit
`awnearleysciosmupsairnegdthbeycDoumnpnuettte'rsstoefsttwuasrienogfTCO.EX.SSTtAepThasnof.twaBroe.dy Nwoeigsthattidstaitcaal analyses were applied to feed consumption data.
Test bird age: 10 days
Pretreatment: None
Test conditions: group Housing and environmental conditions: Indoors in batteries of
thermostatically controlled brooding pens. Floor space of each pen
measured approximately 62 X 90 cm. Ceiling height was approximately 25.5 cm. External walls, ceilings and floors were constructed of galvanized steel wire and sheeting. Identification: Each group of birds identified by pen number and
test concentration. Individuals identified by wing bands.
Number of replicates: Six for controls, two for each treatment
Number of ducks per replicate: five
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Number of concentrations: Eight plus a negative control Feed and water: Game bird ration formulated as below, water from
the town of Easton public water supply. Both provided ad libitum during acclimation and testing.
[mew Game Bird Ration Tm I --o-- [[fr T Errooomwgasw ow w] ew i2 -- A ---- -- ---- --
[[ [ meF e mr t]e --------owm%m----]] [F To a ar -- o --wn -- om SR GATIo TT no
[[oeT Vmimmtoamstiinoaense d Mineral r Premix e | wm] es
[ms LC
ww |
fC e r w -- w O w] w--
[[fow ormmoeem ww | m] oewwe]
[(wToesredreamae) | sosem]]
fw C r oL m] ew
Fon
swewe
PBrroopohdyilnagxicso:mNpoanretment mean temperature: 38 + 2C 001263
Ambient Average
room mean temperature: 25.2 relative humidity: 53 + 18%
+
0.7C
LPihgohttoipnegr:ifoldu:orSeisxcteenetnlihgohutsrswhliigchht pcelrosdealyyapproximate noon-day
sunlight; average approximately 207 lux.
Test diet meansof
preparation: Test substance mixed directly a Hobart mixer. No carrier was used.
into
the
ration
by
bDyiectolslaemcptlinigngsi:x Hsoammopgleensefirtoymotfheth9e.1tesptpsmubasntdansicxefirnotmhethdeieltoweevsatluaantded bhiogthteosmtofcontcheentlerfattaionnd. riSghatmspelcetsiocnosollfecttheed mfirxoimngthveestsoepl,.miTddhlees,easnadmples avelrsiofisceartivoendsaasmptlheesvefrrifoimcatthieonrsemaamipnliensg fcoorntcheensteractoinocnesntarnadtioonnes.frTowmothe. ccoonltlreocltewdeartetchoelelencdteodfatthepreexppaorsatuiroenpoenriDoday(D0.ayS5ta)bfilriotmy tshaempcolnetsrowle(roene sample) and each treatment group (two samples each).
RESULTS
Nominal and 1171
cpopncmentrations:
Bk
control,
9.1,
18.3,
36.6,
73.2,
146,
293,
586,
Measured concentrations: and 1196 ppm
<LOQ,
9.8,
19.5,
40.2,
74.5,
174,
291,
537,
Element
value:
Dietary LCs) No mortality
= 628 (44--8 concentration
958) ppm = 146 ppm
All
element
NOEC (body weight values based on nominal
gain) = 36.6 ppm concentrations
AAnnaallyytsiecsaolfMteetsthosdoolultoigoyn:s wDeiertespaemrpfloersmewdearteWeixltdlriafceteIdntweimtahtmieotnhaalnLotld,. duestiencgtihoingh(pHePrLfCo/rMmSa)n.ceWlhiqeunid dcehtreormmaitnionggratphheycwointchenmtarastsiosnpeocfttrhoemettersitc rsuebssptoannsceefoirn ptehreftleusotrsooolcuttainoenssu,ltfhoenastaemweasanudsemdo.stNporaotmtienmepntt wpaesakmade otfo qquuaannttiitfaytoionn)thweabsas1i.s15ofpipnmdivinidtuhails isstoumdeyr.icThceommpeonaenntpesr.ceTnhtereLcOovQer(lyimoift 9m4a.t7r.ixSfaomrtpilfiecsatcioonlsleacntaeldyfzoerddectoenrcmurirneanttiloyn odfurhionmgosgaemnpelietyanianldyiseitsrwaansged from 102 - 108% of nominal. Samples collected for verification in diet had
061269
measured values from 92 to 119% of nominal. Measured values for ambient stability samples taken at Day 5 ranged from 94 -- 130% of nominal.
os`Summary of Homogeneity
analytical in Avian
chemistry Diet
data:
--
CCro|emm mme|n|n] [oo|ooomome| moo0s|nwws|
Verification in Avian Diet
= = [opmecoa|
qa
TT]
eeee ee] [ow | ew | Ce
wm
ow wm
=re OS =r= Ambient Stability in Avian Diet
| wu | we |
Tw |w | | ww |ow]
[opmecon|
[oe [oe |
eo
awe ear
[|]
To ww | | wo | on|
eee ee Fw
ww Tw[en]
Fw[w Tm w|w]
ew | wm Tw[ww]
Cwe TT
wwe | we | uw]
co1270
Biological observations
Survival and control group,
calnidniaclallboibrsdserwvearteionnosr:maNloinmoarptapleiatrieasncocecaunrrded
in
the
behavior the 1171
throughout the ppm treatment.
test. The Mortality
oficrcsturdreeadthtshroocucguhrrDeadyo8n
day 4 in all
in
pdoosste-egxrpoouspusr>e p2e9r3iodp.pmThwietrheswoemree nofotthreeadtemaetnhts.rbeeliantegddmuorritnaglitthiees
coronocveenrttrsaitginosnsof>
toxicity at 293 ppm
dciosnpcleanyterdatsiiognnss<of1t4o6xipciptmy.inBcliurddisnagt
all
croeodrudcienadtiroena,ctriuofnflteod satpipmuelair(asnocuen,dlaetnhdarmgoytiaonnd),lloowsesr olfimb
pwoesatkunree,ssd.eprBeirsdssioant tahned1c1o7n1vuplpsimonlsevtelhraolusgohdDisapyla8y.edRepcroosvterraytewith
normal appearance test termination
and
behavior
was
noted
from
Day
9
through
wBeordeynwoeiagpphatrgeanitn:trWeahtemnentcormeplaatreeddeftfoectthse ocnontbroodlygwreoiupg,htthaemroeng
Dthaeyb1ir5d-s22inpcoosntc-eenxtproastuiroensp<er3io6d.s6,pbpomd.y wDeuirgihntg gtahienDaapype8a-r15edand
(cpom<p0a.r0a5b)lreedaumcotinogn ailnl wgeriougphst.gaTihnerate twhaes9.a1sptaptimstliecavlellyfsoirgntihfeicDaanty
g0-r5ouapnadtDtahey 95.-18 ppeprmioldesv.elHaopwpeevaerr,todbieffderueencteosaflroowmetrhmeecaonntrDoaly 0
Tbhoedryefwoerieg,htthfeosrethdeif9f.e1repnpcems
level, were
anontdcwoenrseidneorteddotsreeartemsepnotnsreilvaet.ed
bMoardkyewde,itgrhetatwmaesntn-oretleadteidn,cocnocnecnetnrtartaitoinosn
responsive > 73.2 ppm
effects on for Days 0-5;
Day 5-8 post-exposure weight `concentrations > 293 ppm.
gain
continued
to
be
reduced
at
wFaesedaCmoanrskuemdptreidounc:tiWonheinnfeceodmpcaonrseudmptotitohen cionntthroeltgrreoautpm,entthere
groups Gross
> 293 ppm Necropsy:
tAlhlrobiurgdhsouthtatthdeiesdtuddyu.ring
the
study,
half
of
those
tsuoravigvrionsgsatneDcaryop8sya.ndNethcerorpesstyartestuelsttstfeorrmibniartdisofnouwnedredesaudbjweecrteed
csiomliolra,r,emipntclyudcirnogpst,hianncdonedimtpiloyn,galsotsrsoionftmesutsicnlale
mass, tracts.
altered These
spleen
necropsy findings were considered to be treatment related.
01271
ET Te T =ee = Percent Cumulative Mortali
CCE oee[ TC ooTea oTTBooC T TT eoTeeTTeot T TooTo]] CCwe [ TeoTeoTTeoT TeeTeeeeoTTeoTTo]] CCi emToeor TToeToor TToeoTTn weTToaTleoo]]
Body Weight (grams)
--
--
cire,n| Pm | Sn|| o[umc|| oAienme |"|ToSe [aMnie Tae
e mmaee [taL[oeo [a om e Lom [a om|] on]
TI eTwTo a ee oeToe oe
TThweTToolow[ea[Tooe laomlToee Teo]]
CCoenT [we To 0o [on[[ooenw |Te[Toooa]
ConTwTaTnTeLonTom Ton]
01272
Mean Average Feed Consumption
omen, fom |so Icons [oom C[ oTm noe |Te wow[|c oonm [] omar]] CCooer[T0o|[e0T|wwm [m] e] T Ce e[ To |w wo |owww w |] ] TCweTTw w ||o 0[weo [a|w] ConTeTw 1 eeTw]
fm EEm]EE Gross Pathological Observations from Birds that Died in Study pf e a--rs | 5|e2|s= hfm aonamnsem m | |e|s | 0|] +] t p.e r |T 2[Le e oa ] o[ p me o T m [ To s [[52]] bbT T oeoms T L T] ]
01273
CONCLUSIONS
The dietary determined
LCS0 to be
value for 628 ppm
Mallard Duck exposed with a 95% confidence
io PFOS was interval of 448
to
958
pchpim-.sqTuahree svlaolpuee owfatshe2.c1o3n.ceTnhterantoiomno-rrteaslpitoynsceonccuernvterawtaison3w.6a7s a1n4d6tphpem.
cBoansceendtruaptoinonr,etdhuectnioonosbisnerbvoeddy wefefiegchttcognaciennattratthieon73w.2aspp36m.6tepstpm.
Submitter: 3M Company, Paul, Minnesota, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
bDaAvTAAQQUUALLITTYY Reliability: Kiimisch ranking = 1
0000000
REREFFEERREENNCEESS
This study request of
was conducted at the 3M Company.
Wildiffe
Intemational
Ltd.,
Easton,
MD
at
the
OTHER
Last changed: 5/1/00
0127%
