Document DGx1aoRxrozMjYD4mVxv1NZ54
AR 26 _ 0774
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
IESTSUBSTANGE
0000
Identity: rMeifxetrurreedctoonatasinPiFnOgSp,erFfCl-u9o5r,ooocrtaansesauclfoomnpaotenewnhticohf mFCa-y2a0l3sFoCb.e(1Octanesulfonic acid) (CAS # 2795-30-3).
Remarks: The 3M production lot number was not noted. The test sample
is FC-203FC, identified by the laboratory as R1335. Current information
i7n4d.i4ca6t%eswaitteisr,a 2m.ix3t4u%reacorfyl0i.c8f5o%amPeFr,OS1,.71%7a%lkdyileetthhyelrenseulgflaytceo,l2b.u5ty5l%estohedri,um
octyl sulfate, 0.12% sodium triethanolamine, and 0.04%
ntiotlryitter,ia0z.ol0e9.%
sodium
molybdate,
0.85%
cThhaerafcotlelrowiiznegd scounmcmeantrryataipopnlsioefsitmopuarmiitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect toxicity of the fluorochemical componentofthe test sample.
Memon:
0000000
Method: OECD Guideline 203.
TGyLpPe:: NAocute static
Year completed: 1997
SSuppepcliieesr::
Pimephales promelas
In-house cultures, maintained
at
AScl-DETD,
Duluth,
MN.
Analytical oxygen.
monitoring:
Temperature,
pH,
conductivity,
and
dissolved
Exposure period: 96-hours
S`tSapteiasrtimcaanl-Kmaertbheordmse:thTohde. LTChseo vNaOluEeCs wvealrueecsawlecurleatceadlcbuylaTtredimbmyeFdisher's
TEexsacttftiesshtaugsei:ngJTuovxesntialtesstatistical software, Version 3.5.
Length and weight:
Average length = 16.6 mm Average weight = 0.036 g (wet)
Loading: 0.12glL
TPersettrceoantdmietnito:nsN:one
cDailrubtoinonfilwtaert.erT:heDewcahtleorrwinaasteaderCaitteydoffoDru2lu4thh,ouMrNs ptraiporwtaotetrh,eptoesstt.-
DilutioHnawradtneerssc:hemistr4y8:mg/L as CaCO
waren
AlCkoanldiuncittiyv:ity: 41419mgy/mLhoass/CcamCOs
pH:
8.08
001383
Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light
and 8 hours dark was maintained throughout the testing period.
Stock and test solution preparation: The test material was addeddirectly
to the dilution water on a weight/volume basis.
SCtoanbcielinttyraoftitohnestedsotscihnegmriactae:l sOonlcuet.ions: Not noted
NExupmobseurreofvreespsleilcsa:tes4:L
glass 2
jars
containing
3
L
test
solutions.
Number of fish per replicate: 10.
WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e sfitvuedypl:us a blank control pH range (0-96 hours): 7.68 -- 8.08 (control exposure) 7.45-7.81 (1000 mg/L exposure)
TCeonmdpuecrtaitvuirtey rraannggee ((00--9966 hhoouurrss)):: 20.5 20.7 C
119 -- 141 pmhos/cm (control exposure)
Dissol1v1e9d --ox1y4g3epnmrhaonsg/ec(m0(-19600h0oumrgs/)L: exposure) 56..91--88..77 mmgg//LL ((c1o0n0t0rolmge/xLpoesxuproes)ure)
R0 Eswts 00000 Nominal concentrations: Blank control, 130, 216, 360, 600, and 1000 mg/L
Element values: 96-hour LCso = >1000 mg/L. 96-hour NOEC = 600 mg/L
Element values based on nominal concentrations
SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadluoenstrheepmoirxtteudraepapslydetosctrhiatbemdixitn utrheeaTnedstnot the fluorochemical proportion alone.
conclusions
000000
The FC-203FC 96-hour LCs for fathead mg/L. The 96-hour No Observed Effect
minnow was determined to Concentration (NOEC) was
be >1000 600
mg/L.
PSauublm,itMtienrn:esot3aM, C5o5m1p3a3ny, Environmental Laboratory, P.O. Box 33331, St. _ p0 ata0 Qua0 nty0000000000 tReesltiianbgi.litHyo:weKvleirm,isscahmrpalnekipnugri=ty2.waTshneosttpurdoypemreleytcshaalrlatchteercirzietedriaanfdortqhuealsittuydy
001384
lacks analytical solution.
confirmation
of
the
amountoffluorochemical
proportion
in
the
REFERENCES
~~~ 00
"DTihviissitoens,t wDualsutcho,nMduNctaetdthbeyrAeSqculesCtoropfotrhaeti3oMn,CDoumlputahnyE,nvLiarbonRmeeqnutaelstTensutminbger R1335, 1997.
o0 mHER 000
Last changed: 6/26/00
001385
EAnSvcilroCuonrcpootrluTieosnt/iAnSgcDiiDvuilstohn "Ase Sudy IDF 5010068
STUDY TITLE ACUTE TOXICITY OF R135 TO
FATHEAD MINNOW (Pimephales promelas)
DATASTANDARD
;
OECD GUIDELINE 203
-
STUDYCOMPLETED
0
June 26, 1997
TESTINGFACILITY
AScl Corporation/AScl-Duluth
Environmental Testing Division
4444 Airpark Boulevard Duluth, MN 55811
Tel. No. (218) 722-4040
Fax No. (218) 722-2592
AScl Study ID# 5010-068
:
3M Company Lab Request R1335
SSppoonnssoorr: t3aMdCIoDmFpRanIyS
1
001386
Study Title
Sponsor Sponsor's Representative
Sponsor's Lab Request | Testing Facility
1.0 GENERAL INFORMATION
fJ eEmemee
Acute Toxicity of R1335 to Fathead Minnow (Pimephales promelas).
3M Environmental Technology and Safety
Services, Building 2-3E-09, 935 BushAvenue,
St. Paul, MN 55133; Tel No. (612) 778-7452.
Susan Beach, 3M Environmental Technology
and Safety Services, Building 2-3E-09, 935
TBeulshNoA.ve(n6u1e2,)
St. Paul, MN
778-7452.
55133;
'
a
R1335.
AScl Corporation/AScI-Duluth Environmental `Testing Division, 4444 Airpark Boulevard,
Duluth, MN 55811; Tel. No. (218) 722-4040.
`RetentionofRaw Data and Final Report
Location of Raw Data and Final Report
Two years from the date study is completed."
T`AeSsctlinCgorDipvoirsaitoino,n/4A4S4c4I-ADiurlpuatrhk EBnovuilreovnamredn,tal
Duluth, MN 55811; Tel. No. (218) 722-4040.
mRErs,
.
:
001387
EAnSveilr"oCAnomSrepnSoaurldtTyoni1/n0A4gSe5Di0i-1vD0iu0sit6oh8n
2.0 OBJECTIVE(S)
'
To 10
determine observable
tehfefe9c6t-choonucren1tr0a%tiaonnd(SN0O%ECe)ffoecfttchoentceesnttsruabtsitoannsce(,LCRy1g33a5n,d
LfoCrsf)a,thaenadd
tmhien9n6o-whour
(Pimephales promelas) under static test conditions.
G`Tuhiidsesltiundey2w0a3s(cOoEndCuDct1e9d93a)c.cording to the AScI study plan retained by the Sponsor andOECD
3.0 TEST METHODS
.
Test Substance | Properties: R135, orange-yellow liquid, specific gravity = 1.1 (water... |
=1), stable and miscible with water.
`
Test Organisms
Dilution Water
1Te0s0t0cmongc/eLn.traTteisotnss:olu0ti(odnisluptrieonpawraetderbycomntarsosl)a,di1t3i0,on21o6f,te3s6t0s,ub6s0t0a,nacnedtg, 6-L aliquots of dilution water followed by vigorous stirring. | fSoruormceA:ScHIe-alDtEhTy,D jiunv-ehnoiulseefcautlhteuarde.minnows (Pimephales promelas) obtaiZned
Holding/acclimation: The fish used in the tests were obtained from AScI's ainn-dho2u3s,e 1c9u9l7t.ure.ForThtehef4is8h huosuerds ipnrtehceeesdtiundgy twesetreinihtaitacthioend mboerttawleietny Mwaasy 17
0%, and the fish were not fed for 24 hours before test initiation. The fish mean length was 16.6 + 2.5 mm, and mean wet weight was 36 + 19 mg.
Loading rate was calculated to be 120 mg of fish per L test solution. a |
Use in test: Used 10 organisms/replicate and 2 replicates/test
concentration.
5
-i
cSohuermciec:al Daencahllyosirsiniastiend RCaitwy DoaftDaulPuatchk,agMe.N, tap water. The most recenta I
|
CCh4aCrOac,t)eriasntdicas:pHAtofte8s.t08t.imeA,ertahteedwaftoerr2h4adh aprhiaorrdtnoesusseofin48thme gte/sLt. (as__='_ |
D
SSppoonnssoorr:SuIdMyCIoDmIpRan1y3
3
2
001388
C`hEaxpmobseurrse Incubation
Observations
Endpoint Calculations
EAnvsieroCnommenptoarlaoTens/tAiSnegtDbivuistosn AS Sudy TOA 5010068
Type: 4-L glass jars.
Use in test:
3.0 L of dilution water or test solution/chamber.
" During the.
test, kept covered, except when experimental observations were made.
Solutions were not aerated during the exposures.
.
`Duration: 96 hours.
Dafliuloryepshcoetnotplearmiposd.: 16h light and 8 h da_rk, maintained using cool white:
Temperature: 21.0 + 1C.
|
`stBrieoslso,giacanld: dDeaaidlyte-st Toersgtanoirsgmasn.isms with abnormal behavior and signs offi;
Water daily-
tcehmepmeirsattruy:re,(1D)OA,t
ctoensdtuicntiitviiattiyo,n-antodtaplHh.ardAnlelsdseatenrdmialnkaatliionnitsy,we(r2e)
_
`made according to APHA methods (1995).
x
LCyp: Inhibition Concentration (ICp) Method.
LCsy's: Trimmed Spearman-Karber Method.
.
NOEC: Fisher's Exact Test (TOXSTAT statistical software, Version 3.5, {
West Inc., and University of Wyoming).
2
``SSppoonossoarr:StIudMy C1o0m4p1an3y35
`
001389
ESn tyhe eoiFn0Dl 1o09n6
3.0 RESULTS
Test Substance Toxicity (Pertinent data are in | 24-h Cg: > 1000 mg/L (Not Caloulable).
oleh
48h LCs: > 1000 mg/L (Not Calculable).
72:h Cy: >1000 mg/L (Not Calculable).
96-h LC; 116 mg/L (Not Relisble).
`Ranges of Water Chemistry Parameters
96-h LCgp: TM> 1000 mg/L (Not Calculable).
96h NOEC: 600 mg/L.
=
Total hardness (as CaCO; mg/L): 46-48.
Alkalinity (as CaCO; mg/L): 44-48.
Temperature (C): 20.5-20.7.
DO (mg/L): 5.18.8. PH: 7.45-8.08.
Specific conductivity (umhos/om):
=
119-143.
Sorensty 5935
5
001350
EASCvlACiSorndpoyrTie0os4AiS0be1it0t0iD6uola8ns
4.0 TEST QA/QC
veces
Tow
Mtohratnal1i0t%y.in control exposure must not be more
`tTehremilnoawteisotn DmuOstcobneceanttrleaatsiton60at%teosft the air
`saturation value at test temperature.
| "5T7he%loofwetshte DaiOr scaotnucreanttiornatviaolnuemeaats2u1r0edCw.as `fTohristhveal1u0e0w0amsg/mLeascounrceedntartattiesotn.terTmhiinsatsioolne vdeaplrueeswsiaosn bdeildonwot60ap%pesaartutroatsiiognn.ifiDcaOntly affgct
`Test duration must be 96 h.
the toxicity measurement for R1335.
ir
"The test duration was 96 h.
i
Reference
optional).
Substance
Toxicity
Test
(this
test
is
|
Control
toxicity
chart for
testing is
sodium
in Raw
cDhaltoariPdaeckreafgeer.ence
5.0 REFERENCES
American Public Health Association (APHA). 1995. Standard Methods for the Examination of
`Water and Wastewater. APHA, Washington, D.C.
.
OGrugiadneilzianteisofnorfoTresEtcionngoomficChCeomoipcearlast.ioOnEaCnDd DPeubvleilcoaptimoenntIn(fOorEmCaDt)i.on 1C9e8n1t/e1r9,9W3a.sOhiEnCglDon, D.Gy, i Hamilton, M.A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for
Estimating Median Lethal Concentrations in `Toxicity Bioassays. Environ, Sci. Technol. 11(7);714-719; Correction 12 (4): 417 (1978).
UCoSnEcPenAt.rat1i9o9n3.(ICAp) LAipnperaoraIcnht.erpVoelartsiioonn M2e.0t.hod for Sublethal `Toxicity: The Inhibition ~-
WWyesotmiInncg.,, and University of Wyoming. Toxstat Software Package. Version 3.5. Cheyenne, ---
SSppoonnssoorr:SalMyCIoDmFpRan1y35
6
w
0013, 91
TABLE 1.
SJipoimoeotpteaen
ACUTE TOXICITY OF R1335 TO FATHEAD MINNOWS (P. promelas)
| de%ed[|0 Ho [|e0o0 | | oseo || ouow || w wo o 0[ [0o[ro e | saww]]
| ow |sag | sen | sen |sanv |
* Percentages are in parenthesis.
-
LETHAL AND NO-EFFECT CONCENTRATIONS WITH
CORRESPONDING 95% CONFIDENCE INTERVALS
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FT. Sponsor: 3M Company
,
=
001392