Document DGvvgkxKBj8GvNK8XrONMzKbn

A k SLA6 _ <3?3t7 ACUTE TO XICITY TO FISH (FATHEAD MINNOW ) TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-601. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was cc 835-34. The test sample is L-9235 (FX-601). Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 81.25% water, 2% propane Sultone foamer, 1% sodium octyl sulfate, 1% sodium decyl sulfate, 0.1% N-(3- chloroallyl) hexaminium chloride, 0.8% xanthan gum, 0.8% starch, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: Not given. Type: Acute static-renewal range-finder GLP: No Year com pleted: 1984 Species: Pimephales promelas Supplier: Commercial supplier, Brady, NE. Analytical m onitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours Statistical m ethods: LCso values not calculated based on study results. Test fish age: Juveniles Length and weight: Average length - 35.4 mm Average weight = 0.312 g (wet) Loading: 0.52 g/L Pretreatm ent: 250 mg/L Tetracycline HCI. Test conditions: Dilution w ater: Carbon-filtered well water. Dilution w ater chem istry: Hardness: 250 mg/L as CaC03 A lkalinity: 240 mg/L as CaCOz Conductivity: 350 pmhos/cm Lighting: A daily photoperiod of 16 hours light and 8 hours dark with a 15 to - 30 minute transition period was maintained throughout the testing period. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Test solutions renewed. Stability o f the test chem ical solutions: Not noted. 002093 Exposure vessels: 1 gallon all-glass round tanks containing 3 L test solution. Num ber o f replicates: 1 , except for highest concentration and blank controls. Num ber o f fish per replicate: 5. Num ber o f concentrations: five plus a blank control W ater chem istry during the study: pH range (0-96 hours): 8.0 - 8.1 (control exposure) 7.8 - 8.1 (1000 mg/L exposure) Tem perature range (0-96 hours): 20 - 22 C Dissolved oxygen range (0-96 hours): 6.5 - 9.4 mg/L (control exposure) 0.5 - 9.4 mg/L (1000 mg/L exposure) R E S U LTS ________________________________ ____ ____________________ Nom inal concentrations: Blank control, 1 ,1 0 ,1 0 0 , 500, and 1000 mg/L. The blank control and 1000 mg/L exposure solutions were evaluated in duplicate. Elem ent values: 96-hour LCso = >1000 mg/L Element values based on nominal concentrations Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS _________________________________________________ The test sample 96-hour LC50for fathead minnow was determined to be >1000 mg/L, the highest concentration tested. Ten percent mortality was observed in this concentration when the replicates are viewed as combined. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking = 3. The study is a range-finding, with limited test organisms. There is a lack of characterization of the test sample and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solutions. REFERENCES______________________ :_____________________________ 002094 This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number B 1 6 8 4 ,1984. O T H E R __________________________________________________________ Last changed: 6/26/00 002095 ENVIRONMENTAL LABORATORY CHEMICAL ENVIRONMENTAL EFFECTS EVALUATION TOXICITY TEST: SUMMARY REPORT Chemical T e s t e d ^ ? Type :if/W f/C - L C ^ / E C 5 o /IC5o/GR50 0 5 % C *L . ): ^ / O /D Test Species: > 7 tte:T e s t Dal /> A //A ///M < A 3 / ^ - A ^ / ^ V ^ L . R . : __________ /& & * /- / _________ ___ Analyst(s) (Exposure/Test Conditions) Exposure Type: Static Static/Continuous Orbital Shaking Static Recircuatory_ Static Renewa Flow-Through Diluent: Aerated Well Water (Initial D.O._^^/mg/l; pH f),f ; Temp. ZjQ C(/^>^F)) Water Hardness: sCj5>f) ppm Hardness Class ificationr-p^^^3/)___________ Dissolved Oxygen % Saturation: Control(s) Lowest Experimental Dose Highest Experimental Dose (coo pH Levels: 48-hr J&L XL -A-. 96-hr -2SL M- XL Extended Exposure 48-hr 96-hr Extended Exposure control(s) Lowest Experimental Dose Highest Experimental Dose 1000*^ Test Temperature C (F): .r> ,0 Y.6 8,0 _8.0, -% & 48-hr 96-hr Extended Exposure X^CC/ZJ> C( ) Photoperiod: Sixteen hours light with a 15-30 minute transition period. 002096 Mk*AkWiU'UJ11J.AU UXliJU1\A1VJIM AQUATIC TOXICITY DATA SHEET ype y ial TB8ted / - ^ (k*6n/ ) a * / r ^ ' ^ V - Q ^ est R e c ' d ^ ^ ^ ^ / B a t c h _ ^ 7 ^ vg.Wt./7A ^/J^,grairs Avg. Sizq ^ *y^cm No. Organisms per dose v Loading Factor 7, 97 ish Disease Treatment^ ? //n rf $ / ? / / / 'Z s / Z y v / ~ f __________ ;________ _ est Solution Volume^/^) m ters/All-Glass ifetrik Tank Dimensions: 35x20 an and 25 cm dee p 1 ixne Started / / ,,/0/)mXlA /(Date Started //?////*& '* / Date Completed , , / / < / & l u `u 1 ^-/ / / / 2 z r ~ /i7 r/m e ^ < ? 7 - ilution Water Carbon-filtered m *1H waf-^T- _^Temp._______ *C., D.O._______ ppm, pH____________) investiga Full S gQ (95% Confidence limits) > / n H D __ 24 Hours 48 Hours 72 Hours 96 Hours 'to. Cone. Amount Total No. pH D.O. Total No. pH D.O. Total No. pH D.O. Total No. pH D.O mg/1 Added Dead PP Dead PETM Dead ^ ppn Dead PiTM +*\K T Control n I T Potkivt -- 1I .o IP n p,r 1 f> <9 inM 1,1 . o p ( { 1,1 n .C o U ' (M ' P-Z (n S V ' kZn (' .: o ... ..j f i IK nti M 3,0 m ftp o t\,C f t / o . r,r A c l ._J % O ...- I f 1CO.. =*)o .. n y. / /0 -iifii o WK Pr it r> ~r{ i n r > 1?[ r . y,(.i n o c (PII 1] c YZ /,? ( j a i n o Q 1X>(? o iaco SBL n 19 <Tr/ y& fi t __ i___ /(? 1$ on a\ Z,(rr , ( t 5 / O H: /n / m V.l Test Tento. C Initials & Date an ___ Z..Mt___ 2 2 2 U Z?nC ?ZCT' 1 7 9 '-J ( /i L -vO'*- // iM, 1<7 **7 nY acondary Effects (other than Mortality): Tank No. Symptoms or Reactions nntrol r d - x 24-Hr. , / zz /1 . \Z rTO 1 /Vy, s?L> su . Jl 4<ia M V /A '6 ____ I V> _ EC5o_ BtSI L W Y AVAILABLE Total Number Affected 48-Hr. 72-Hr. 96-Hr. ./ U' rnr / ._ // a1 /. i / . \ S f\ ~S< -fXM C //i/< h Zj / ? " 110205*1 F.orr.i 10620-8-B PWO Lab Request No.' _ Page____/____ of___ /_ Environmental Lab -- Work Sheet 7-19-84 ENVIRONMENTAL LABORATORY STATUS REPORT ALL RESULTS TENTATIVE LAB REQUEST NO. B1750 QUESTER NAMES RRR PT: 0222 OJECT NO: 06 TE RECEIVED: 6-14-34 SC: MAY-CARBON FILTERED WELL WATER CONTRACT LAB: CONTRACT LAB COST: 0 3M E-L HOURS: 3 EST CMPLT DATE: 7-27-34 DATE COMPLETED: SAMPLE DATE CODE DESCRIPTION TEST RESULT 1 6-01-34 RM# 4 2 01 WATER PH RES-CL COND TOT-HARD TOT-ALK TSNH3-N SOL-F COD-LOW TOT-COLIF SPC PH RES-CL COND SPC 7.9 <0.02 MG/L 350UMHOS/CM2 240 MG/L 240 MG/L 320 MG/L #<0.4 MG/L 0.2 MG/L 4 MG/L NEGATIVE 49 PER/ML 6.8 <0.02 MG/L 2.1UMH0S/CM2 9 PER/ML # = UPDATED DATA * = CONTRACT LAB 002099