Document DGmR21qYa7Og4bR4J7bM4a7Bo

| . 20f0 3M 8EHQ-0202 _00373 SKaUtTheVriineehE. eReend, Ph.D. 3nMd ESnvaiyronSmeerntiaelsTechnology L[oeEr 9SB700u0UonBMuxgsAh52N.A3v3e8Sn.u3eS6 maAR 226 _ 1080 CERTIFIED MAIL HEHY-o- B73 February 1,2002 Document Processing Center (407M) EPA East - Room 6428 Attn: Section 8() Office of Pollution Prevention and Toxics U12S00EPPeAnnsylvania Ave. NW Washington, DC 20460-0001 Cone,Shiianiie CBJ 3 Re: TSCA 8() SUPPLEMENTAL NOTICE ON: Perfluorooctanesulfonate Docket Nos. 8EHQ-1180-374 Dear Sir: R 3M `and hPaesrfrleucoeriovoecdtadnreafstulrfeopnoarttes o(fPrFeOprSo)dfurcotmioWnilsdtluidfieesIonftenmoarttihoenranl,boLbTwDh.i,tienadincdatmianlglard = effects as described below. The studies were done according to FIFRA Guideline 71-4 I and OECD Guideline 206 and followed a pilot range-finding study that was deemed Pl om inconclusive as week feedings c to on treatme taining nt-rela PFOS. t ed reproductive effects. The Draft histopathology results studies were arc available ba at sed on 21- this time, = <= 5 - but analytical resultsof serum and liver levels will not be obtained for at least two : : months. Therefore, it is not yet possible to correlate the findings described herein with present levels of PFOS in wildlife. - 2 1. Mallard Dietary Study Results: 16 pairsof ducks were exposed via feed for 21 weeks; hatchiings were reared without exposure for 2 weeks. The S0ppm feed level group was terminated at week 7 due to overt signsof toxicity. but terminated at week The 150ppm feed level group was dropped to 20ppm at week $ due to overt signsof toxicity. The 10ppm feed level group 3, showed no difference in reproductive performance from the control, no treatment-related `mortalities (adult and young), no overt signsof toxicity, no body weight or liver weight differences from the control, no difference in degeneration/atrophyof seminiferous {ubules from consumption the control, differences no difference in from the control eggshell thickness from the control, no feed and no offspring differences from the control offspring. However, reduced testes size was noted in 2 control males and 7 treated males. Histopathology showed 1 control and 4 treated males had decreased spermatogenesis. sz NURIA The pathology report noted that the reduced testicular size, with or without microscopic evidenceofdecreased spermatogenesis, is a feature most consistent with carly post- epa-ors 1 0008118420 Office ofPollution Prevention and Toxics PFeabgreuNaor.y 21, 2002 reproductive phase regressions, a degeneration/atrophy noted in the normal control physiological phenomenon. and treated adult birds may The simply represent a ater stage of this normal change. However, the increased incidenceofreduced size: sreuggrgeessstisotnhat treatment may have accelerated the normal post-reproductive phase 11. Northern Bobwhite Quail 16 pairs without of northern bobwhite exposure for 2 weeks. quail The were exposedfor SOppm feed level 21 weeks; group was hatchlings were reared terminated at week 7 due to overt signsof toxicity. The 150ppm feed level group was dropped to 20pm at wgreoeukp3shanodwetdernmoinaadtueldt atfrteeartmweenetk-r4eldatueedtmoorotvaelrittsy,ignnso obfodtoyxiwceiitgy.htTdhieff1er0epnpcems ffereodmlevel control, no feed consumption differences from thickness from the control, and no differences control, no differences in eggshell in spermatogenesis or morphology of testes from control. However, there were treatment-related effects: - reduced reaction to stimuli, ruffled appearance and lethargy (adults) -~ offefmsaplreinlgivseurrwvieviaglhtrsatweewraeshaifgfheecrtetdha(nfecwoentrrnolusmb(aedrulotfs)14-day survivors per cggs set) necropsy indicated reduced testes size in | control male and 7 treated males (adults) No other reproductive end points were affected. "The pathology report noted that the reduced evidenceof decreased spermatogencsis, is a testicular size, with or without microscopic. feature most consistent with early post- reproductive phase regressions, a degeneration/atrophy noted in the normal control physiological phenomenon. and treated adult birds may The simply represent a Slrauetggergreessssttiasognet.hoaftthtriesatnmoernmtalmacyhahnagev.e aHcocewleevreart,edtthheeinnocrremaasledpoisntc-irdeepnrcoeduocftirveedupcheadsesize Twavihateihlaftbihlneea,laGnaLlyPtisctauldireespoarntds the data from the on sera and liver cpoinlcoetnsttruadtyiownislol fbePsFuObSmiatstesdootno EPA, along. as they are For further information, please contact me at 651-778-4331. Sincerely, LaZzherin &=ne Katherine `StaffVice EP.reRseiedednt, Environmental Technology and Safety Services L