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AR126-052Y4 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: PaesrPflFuOorAootcettarnaoibcutayclida,mmaomnmioumnisaultm, sAalmtm; omnaiyuamlso be referred to paesraflmuaojroorocctoanmopaotneePnFtOo,f FFCC--111061,5FoCr-1F2C-61,0F15C--X1.69,(OFcCt-a1n4oi3cor acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Rcheamraarctkesr:ized,Ththeotuegsht scuarmrpelnet iinsfFoCr-m1a0t1i5o-nXi.ndiIcta'stepsuriistyaw3a0s%nsottrasiugffhitciently cwaarsbo"nHcOhGaEin2v0e5r"s.ion of FC-143 in 80% water. The 3M product lot number The following summary applies to the test sample as a mixtureofthe tceosntcesnutbrsattainocnes ionfwiamtpuerristoilesu.tioDnatwaitmhaiyncnoomtpalcectuerlaytcehlayrraecltaetreitzoexdicity of thetest sample with that of the test substance. Data was used to `compare toxicity of the branched/straight chain`ammonium pseurpfplousoreodoctotabneoatthee h1o0m0o%lsotgruaieghmtixctaurrbeoinncFhCa-i1n4a3mvms.onwhiautmIs perfluorooctanoate in FC-1015-X. METHOD Method: OECD 209 Test type: Static acute GYeLaPr:CNomopleted: 1996 Analytical Statistical mmoentihtoodrsi:ng:NotDiaspspollivceadbloexygen concentrations and pH. Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN Test condition: Dilution water: Millipore Mili-QTM water Synthetic sewage: per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Mill-QTM water, with 1N H;SO, then brought and diluted to t1oLtwhiethpoMinitllof-QinTMciwpaiteenrt. precipitation The pH of twhaesraedfedreedncdeirseocltulytito0ntwesatsvmesesaeslus.red to be 7.2. Test substance Test vessels: Not stated 04040 Number of concentrations: 4 plus 1 reference substance and 2 blank controls TToetmaplersautsupreen:de1d9.so1l-i2d2s.a1nCd pH on day of testing: 3.22 glL (TSS in final 1.3 g/L) and initial pH 7.8 and final pH 7.9 Element Basis: Respiration inhibition as determined by oxygen consumption. Remarks: Values corrected to 20C for calculations RESULTS Nominal concentrations: 2 Blank controls, 420, 840, 1660 and 3320 mg/L Element values: . 30-minute EC50 = >3320 mg/L 3-hour EC50 = >3320 mg/L. Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply. to that mixture and not the test substance. CONCLUSIONS dTehteerFmCi-n1e0d15to-Xbe3->h3o3u2r0EmCgs/L.for activated sludge respiration inhibition was SStu.bmPiautlt,erM:inn3esMotCao,m5p5a1n3y3, Environmental Laboratory, P.O. Box 33331, DATA QUALITY Reliability: Kiimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request N2169. orem 00000 Last changed: 5/25/00 04041 JESTACVOA P20Y1 .. EVROWNTAL USORATORY ` PSRfaPIroiaeTrsus ] Tree Test OR ECOE ACTIVS AATCETDIVQ ASTEtDvSoLR UcDeGE MA IXEDLIT QUOR ITnO kil'N diown eTeLstVL20q b ACTs1Vu0GAEuTeDELuDon Date' Obtained 4-25-95 Date Uses 4-25-75 MadUsSpend Sekds 001593320 on. Adstnd 10 on vss i altar soiton_1.3 gn. Brg Inidal pH 1% (15t test soln. prepared) Final pH 79 ast soln. prepared) EF comments f IESTsussTance Sample Description FC [015 =x Init BH of hes conc. tested (Song) 72 Commants _-- Adus1epdn _= : Simole Deleipior 13,6 - Bictiarepbesal PGE ; nial pH of stocksatan Cd es (Sco mo) TeCCA adtR edCE= 4 a Eoxdpd osuer xcoe no pmoa nn s ie TEE TI Fenner ie I a B ET R ps Mere oo GATE Seo RASRR ET + ME Es Be RE Sr3 Raa FRTEYa RASRR SE Ek on ae rok 5 E oAiee R JBEBG GS dut Sseony BSimeT Saee ai SLE c0R04R Ro en AE i ebe2508 cat : a Pla5e. H oe AcmeENsVnIiROsNtMeENsTALe. LoABeORseAwTnaOmRYones xeCaieeT Tost Substance: F _FC= 1OVS-e x es asipngele Duscipion: Clee Tus liquid owe: 4-25-96 (3 ZTocao, tmtedn CAaMean mm omsEee emSc. Tova Ba=Se gedr io mes See CWaTe ies =o Sm E ma Te wee me w la Semes THTeIes e de e meeTeas e Tal of wer eerdeamg omTE eeele A HSoe mmap nenr Se Gae e veTT ii T e arm ein aa e eyee R 0a Map rs E 8 te pA y eCe5 nS7 ofet i a pp SO A E riene h i ie ss ET Boda 3 FL i ee Bhi SRShaPki i i erG> nii EGR RaTaeRE Fes Gist E Anha043 de BESTCOPY AvAU sous : ot ENVIRONMENTAL LABORATORY RPeiadeiTe OECD ACTIVATED Aan oar SLUDGE RESPIRATION INMBITICN TEST #208 Ei Tos suvsance: FC 1015 X Source of Activated Siudgo Med Lauer: Mites be osBocas: 135 -C- Anaya: NS LeeTee tmentPlant,$480 oatUsos_4-25-6 11 % ii JMNUTE EXPOSURE = CSSOLVED OXYGEN CCACENTRATINS, mot ; TCoenacresrubssaonnctsegiafo5 . [19 {15 2T5EV5I0N]OTESas 0s [50 ToErTeR:e lalla] PY SYPYPO IP Sac 3d 7[wee alol a] sal 5 Id1 al9c dol3e| av. TSHoiecc stTaondelr es srfenlu ba Ted[ealar 6s ralen bes) 3a1av oe[dalsila]g aZa0o TT rrr Tee SRST| a FY Tenceanseatons IP) PR IIEE FERRY ; T : w: rs aoaT[oisoTlias re YJRa--eES mEsYIya[e. eEe[P531PsRPFaRE72Hlvyalaa n[E1k2 a yTdecso l{eeohlsAaTlseanSsolTedaeTlo4oab liageasit s38i 20Iaeo reeF mn nbts a or soio lgsoales wyaatel7y3i]| [3:i3c T[intF s [Vt ai vFiool[e0v0s0acreas[dam ST TT TT Cenie lese] ess =i -- Eewi 3a| 79] ve] 2a | oa ul oalln ie] EA + oonm ofsaeartesdsuoegompisead iirCa mia Ses amour Tre 28 | LS 18 SABETAINAN HE SEBodS hi S NRREES 1 E SR SE 0 He irda RC i fe L SEE LE i 04044 Gc 3 PEERESERl coowmnsermom an jo Bien oi 5 i te TE osc go3hy FbHoECSohEr r m nr s En Re Re CeLses He e he e e eeT e a ee e e Te e e e a] ele e 52 gE l sppe epreeprn ehbeeTaLeeerefoseu)nd 3= fs Ee brotor rte tor] Trn fet a ESee o=mF --] s a hid A ee arTeESeeamen me ) P baeEhngae blne ereepepePpr RirRR Eh eRDeRbeeeRba au beS ane f TLFe eosaen efeee--] er--]]| pede 2 BN Po5~ pB [PsEsi essmt E sr amS a eei Theeta ar tata atat teti ml t -- 3E: wo [EEE Cr i igsd REsCA ee 55 ch oR BEST COPY AVAIl ABLE [E---- w ey Pleat oto AGTVATED SUDGE SFeETSPRoATOoN RtUGION TEST20 oon. Brick - G0 tAbcL Tot Substance: 3.5" Dichiloce henol lob Godloxe opuo:orLTdKSSTe PSrpoeapnairsatoioiino:na5S0o0o mllgIL;Lo-1 OOdmmeee 80.85 oma ro = =- ] CL e EER r eg mee o T=imer--mstiagmeed wrmaE7 wDeLTT E PeRda sEaeSmoSina =ea=r ] = = 75a r =-- =eEr se fy -- f Ee e bibl, BE m A ese seso em e TsAL s TTRIAMNBRr E h is IE ee veeeee am ST TI CnWAvp wo FE Gon TE eRR oland 53 iG es) n ead A RE A SA Ra a1s ) % ah 3 w SE 5H 7 copie > nToga + T Egm Bena s fn he nelgi ii BESTACvOa Ps5Y:5 - ENVIRONMENTAL LABORATORY AaPleea 060 ACTIVATED SLUGGE AESPRATON CBC TEST 200 awoan TSeosutrceSuofsuAnctcieva:ted3OS.i5udi:ge McixedbLiqul or: (oMakersWes: WpontahrTeesdmmendaPlolt, StParl; Mn) J Oi Rocoved: 4-32-10 atoUses: 4-25 76, SOMNUTE EXPOSURE ~ DSSOLYED OXYGEN CORCETRATINmoSt,. TCesotnSuabsntatnce.ra] Tens TRAEMINUTES. so] ss] [Temp wm 1s [re |e los] es olen [elu face e---- 1g [oi] Leiperlonfog] 210 i --T7 1 1 7 A EN | T rT rr r TTT we [T JE]es| cofse [eaT lwT alr r a] T | eT alou1 Jao | mE ime = HOUREXPOSURE" FY. DISSOLVED OXYGEN CONCENTRATIONS, mot: S Ts ieegolosTT[a a| zo [TE FoO TT e aeip] 0] 50Tr| 0 FELTET| [TSE5T0:ne[ea er[coTcEoEtR Tuc- | os i4f c To Leidnv fOmpdebGooanlee|Doe|srselaolaksttiGln bais || .1 dos at oneal [om [7 [|| |[oe 5 {xTe= Rai|| <7[OSAh][STORR |7| | ||||| a |ORWR A |DpORa| 55ec || 5 Ao | F=e es[m yran [[evaosa nkae |w0 el|sea] eoev|eroeel@wRBl[imreea|rls [1|91i ootrR aE Sedo Er,RCs3 EE SEES RR TERT Tak,Lg Fost emporairer iat ti ane sata Re EEL E5 : B% 04047 4 % ERA 5 AB Biomol BLcriosmeneonconorr of H3iiEf Me Bore [[ epa iI Dase a re epe ereepas eeT e sepespe eee Lbap se e Fea Roer TeoLaeea o oe daapay e ) i=EF% RE E E EET be r berbere ber orFe acer --a = 4 BC Srer hprrenIEr pR epeErpEerfeEefr E beorr pcr ortteorbE oroe stfeoea nnroee nbalaas 2ft: gs er r a =t rtf|--tt--t--tbt : 5 StEeee t bebototal totemfLmEt--E > TE 30 EEE bs aon Ho h s Ree eese Sli rN Ph = oo. MJ F ee 2