Document DDb9QJRgbqYvEvee8b2M6rZ9M
MICROBICS MICROTOX(@ TOXICITY TEST
TEST SUBSTANCE
Identity:MixturecontainingPerfluorooctanesulfonatmea;y alsobe referred toas PFOS, FC-95, oras a component ofFC-3072. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was not noted.The testsample isFC-3072. Currentinformatioinndicatesitisa mixtureof 1.67% test substance,33.33% diethyleneglycolbutylether,29.23% water,20.83% urea, 5% sodium octylsulfate3,.33% sulfonatedalkylethersulfatesalts,4.60% acrylicfoamer,1.67% triethanolaminea,nd 0.33% tolytlriazole.
The followingsummary appliesto the testsample as a mixture of the testsubstance in water solutionwithincompletely characterized concentrations of impurities.Data may not accurately relatetoxicityof the testsample with thatof the fluorochemical component.
METHOD:
Method: MicrobicsMicrotox@)"BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1995 Species: Photobacteriumphosphoreum Analyticalmonitoring:pH and osmoticcondition. Exposure period:30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotox@) Test organism source: Microbics'Reagent. Test conditions:
Diluent: MilliporMeilli-Q"wmater Test temperature: Not noted. Stock and testsolutionpreparation: A 2000 mg/L primarystock .solutiownas pre ared by dissolving200 mg testsubstance in100 mL MilliporMeilii_QTw@ater. A secondary stock solutionof520 mg/L was preparedby dilutin2g6 mL ofinitisatlocksolutionwithwater. The pH ofthestocksolutionwas 6.8.An osmoticadjustmentwas made using 200 mg NaCl dissolvedin10 mL stocksolution.Alltestsolutionswere made by proportionadlilutions
Exposure vessels: 4 mL cuvettes Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESULTS
Nominal concentrations:Blank control3,2.5,65, 130, and 260 mg/L
Element value:
5-minuteEC5o = 305 (282 -330) mg/L 15-minuteECBO = 184 (177 -192) mg/L 30-minuteEC50 = 139 (134 - 144)mg/L
Element valuesbased on nominalconcentrations.
Remarks: Testingwas conducted on themixtureofthe testsubstance as describedinthe testsubstance remarksfield.The valuesreportedapplyto thatmixtureand notthefluorochemicaplroportionalone.
CONCLUSIONS
The testsubstance 30-minute EC50 forPhotobacteriumphosphoreum was determined tobe 139 mg/L witha 95% confidenceintervaolf 134 to 144
mg/L.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking= 2.Thisstudymeets the criterifaorquality testing.However, sample puritwyas notproperlycharacterizeadnd the study lacksanalyticaclonfirmatioonftheamount offluorochemicalproportionin solution.
REFERENCES
Thisstudywas conducted by the3M EnvironmentalLaboratory,St.Paul, Minnesota,Lab Request number M3597, 1995.
OTHER
Last changed: 6/25/00.
06/25/2000
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
LAB REQUEST NO. M3597 REQUESTOR NAME: SBS/KEN GOEBEL DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 11/29/1994 DESC: FC-3072 AFFF BASE
CONTRACT LAB(S): ASCI EXP COMP DATE: 02/06/1995 DATE COMPLETED: 03/24/1995 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176
TESTING IS TO BE DONE IN-HOUSE.
Sample sent to contract lab for toxicity to fish evlauation due to problems with lab fish cultures. SAB 2/20/95 Results from Microtox testing:
Test 32.5 65 130 260
Conc.
mg/L
EC50 sab 3/16/95
Percent Light Loss as Compared to the Control
5-min exp.
15-min exp.
30-min exp.
10
12
15
16
21
27
27
37
47
46
62
74
305
184
139
Results from Fathead minnow toxicity test: After 96-hours of exposure, all test fish were still alive in 1000 mg/L test substance.
SAB 3/22195
Activated Sludge respiration inhibition test results: After 3-hours of exposure, there was 15% inhibition in respiration at 1000mg/L SAB 3/22/95 Results from Daphnia magna toxicity test: No mortality or immobilization was observed at 1000 mg/L after 48-hours. SAB 3/23/95
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SAMPLE DATE
CODE
DESCRIPTION
RESULT
MDL or (95% C.I.)
--------------------------------------------------------------------------------
1
FC-3072
LIQUID
ASCI
48-HR-EC50 96-HR-LC50 MICROTOX SLUDG-RESP
DAPHNIA ACUTE TEST *FISH ACUTE TEST - 96 HOUR MICROTOX - EC50 SLUDGE RESPIRATION - EC50
CONTRACT LAB DATA
>1000 MG/L >1000 MG/L 139 MG/L >1000 MG/L
(134 - 144)
ENVIRON,NIENTAL LABORATORY:
LNIICROTOX PREP SIIEET
TEST SUBSTANCE:
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LR: Date: Analyst:
1. PreparationofStockSolution
.Vfa,5s,"TVeosltS.ubstance: FinalDilutionVolume: Diluent:
FinalConcentratioonfStockSolution:
2.pil Adjustment of Stock Solution
* InitiaplH ofstockSolution: * pH Adjustedto: * pH A djzisteidviih:
3.Osmotic Adjustment of Stock Solution Osmotic Adjuster:
*Comments:
4.Appearance of pH and OsmoticaUv Adjusted Stock Solution
CLEAR or OP.4QUE Color..
(circloene)
Comments:
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