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. AR226 09/9 MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-3104. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FX-3104, 6%. Current information indicates it is a mixtureof0.48% PFOS, 14.09% diethylene glycol butyl ether, 59% water, 17.6% ethylene glycol, 1.44% sodium octyl sulfate, 4% inhibitor, 2.0% polyoxyethylene smuolnfoatoec,tyalnpdhe0n.y1l%ebtehnezro,tr1i.a2zo5l%e.propane sultone foamer, 0.03% sodium lauryl The following summaryapplies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical componentofthe test sample. METHOD: Method: Microbics Microtox "BASIC" Procedure. GTeLsPt:tyNpoe: Static acute Year Completed: 1985 Species: Photobacterium phosphoreum AEnxaploytsiucrael pmeorniiotdo:ri3n0g-:miNnouttegsiven. Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Not given. TSetsotckteamnpdertaetsutrseo:luNtoiotnnoptreedp.aration: Not given. Exposure vessels: 4 mL cuvettes. NNuummbbeerrooffcroenpcleincattreast:iotnwso: Four plus a blank control Element basis: Percent light loss RESULTS Nominal concentrations: Blank control, 100, 250, 750, and 1000 mg/L. Element value: 56-minute ECso = >1000 mg/L 15-minute ECeo = >1000 mg/L 002998 Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reportedapplyto that mixture and not the fluorochemical proportion alone. CONCLUSIONS The test substance 15-minute ECso for Photobacterium phosphoreum was determined to be >1000 mg/L. Submitter: 3M Company, Paul, MN, 55133 Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY _ Reliability: Kiimisch ranking 3. Study lacks description and recordsofthe method followed. There is no characterizationofthetest substance purity, and the study lacks analytical confirmationofthe amountoffluorochemical `components in the solution. RENCES This study was Minnesota, Lab cRoenqduuecsttednubmybtehre 3C1M11E1n-v2i,ro1n9m8e5n.tal Laboratory, St. Paul, QUE Last changed: 6/26/00. emer. 002999 "0 ENVIRONMENTAL LABCRATORY - Microrin, Toxierry Test WDRebfbas : MicrorRAw.Data AustLat Same DescriPronFre2IoM0% Tye Lightwader ee]reFE e Bl Bans X10 w | 23 | + . ca Durticate lo | |e B2 loo cz duplicate B83 250 C3 duplicate : | 302 |p ss faq |p | le | 3 | 8 1 | 29 |2a | 2 ay #50 cy duplicate oles be | w 20 |H |9 B5 1060 cs duplicate. Bolas 9 eo 1+ | n * 003000 . FUCROTOX (DATA TNaRUC HON Red =2. pd Bran . Ramax* =-fich DATELehgs Advis Kaine Ryans Cuivetrs lan haifa Poa Cis fcc Renal fe 81 THe| C1 1% aul 0 THe Bran Ramos row, BL And CL SHowd AGRE. WITHIN 0.02, Mean Bian Rann = Bd Ra Rabin RICRabin cos NoruaL izes Lagu boss (Nepreer) =Total Zogcto Z B3 - -4 =H =.399 -.aty -. 318 - 430 -.890 - tio B5 Cs Po bent 21S L248 Laut -.095 ots 0 hass (Yo A) =133r X100 2 = 3k - 302 - 23 BY : 59, 15.6% BS 1.9% A da +27 4.5%, [Erec ce Concenaeano ['sovm : |ism | [" &Ceo 1" [Reee ~~ : [ T---- ( Etm>i000 =o 003001