Document D1G5nD8GEvx079b2OwgqD25Q
ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE
Identity:MixturecontainingPerfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-206A. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was cc 785-22. The testsample isFC-206A. Currentinformationindicatesitisa mixtureof 0.92% PFOS, 18.75% diethyleneglycolbutylether,75.44% water,1.83% Hydroxy foamer, 1.75% sodium octylsulfate1,.2% poloxyethylenemonooctylphenylether, 0.06% sodium laurylsulfatea,nd 0.05% benzotdazole.
The followingsummary applies toa mixture with incompletely characterizedconcentrations of impudties. Data may not accurately reflectthe toxicityof the fluorochemkal component of the testsample.
METHOD:
Method: Not given.
Type: Acute continuousflow
GLP: No
Year completed: 1979
Species: Pimephalespromelas
Supplier:Commerical supplieirnBrady,NE
Analyticalmonitoring: Temperature,pH, and DO. Exposure period:96-hours
Statisticamlethods: LCm valuecalculateudsingprobitcomputer program. Test fishage: Juveniles
Length and weight: Average length 41 mm
Loading: 0.64g/L
Average weight 0.64g (wet)
Pretreatment:None
Test conditions:
Dilutionwater: Carbon-filterweedllwater
Dilutionwater chemistry:
Hardness: Alkalinity: Conductivity:
236 rng/Las CaC03
221 mg/L as CaC03 430 pmhos/cm
Lighting:Notgiven.
Stock and testsolutionpreparation:A primarystocksolutiownas prepared at2lO,OOOmg/L. General dilutiofnactorof70,equivalentto3,000
mg/L. The primarystockwas proportionalldyilutedwithdilutiownaterforthe additionacloncentrabons:dilutiosneries100%, 60%, 40%, 20%, 10%, and 0%.
Flow-througrhate:Approximate4l.y5volumeadditioonfstestsolution every 24 hours. Concentrations dosing rate:Contnuous Stabilityof the testchemical solutions: Not noted. Exposure vessels: Glass tanks (36x2lx24 cm) containing16 L oftest solution. Number of replicates:1 Number of fish per replicate:16 Number of concentrations: fiveplusa blank control Water chemistry during the study:
pH range (0-96hours): 7.9 - 8.0 (controelxposure) 7.6- 8.0(3000 mg/L exposure)
Temperature range (0-96hours): 18 -21 *C Dissolved oxygen range (0-96hours):
7.0- 9.3 mg/L (controelxposure) 4.5- 9.3mg/L (3000 mg/L exposure)
RESULTS
Nominal concentrations: Blank control3,00, 600, 1200, 1800, and 3000 mg/L
Element values: 96-hour LC5o = >3000 mg/L
Element valuesbased on nominalconcentrations
Remarks: Tesbng was conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsample 96-hour LC50forfatheadminnow was determined tobe >3000 mg/L,the highestconcentrationtested.Thisconcentrationcaused a 12.5% mortality.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking3. Testinglacksrecordofmethodology used. There isa lackofcharactedzabonofthetestsample and thestudylacks analyticaclonfirmatioonfthe amount offluorochemicalproportioninthe solution.
REFERENCES
Thisstudywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number 4970S, 1979. OTHER
Last changed: 6/26/00
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