Document Bym8ON3npEz4xyZ2OXn344Ob8

% AR226-2836 CCopies to : E. I . du Pont de Nemours and Company aH a sk e ll L ab oratory f o r T o x ico lo g y and I n d u s t r ia l M edicine HASKELL LABORATORY REPORT NO. 275-73 Material Tested Q Haskell No. 79I+3 Other Codes Submitted by Organic Chemicals Department PRIMARY SKIN IRRITATION AND SENSITIZATION TESTS ON GUINEA PIGS Procedure- The t e s t f o r prim ary i r r i t a t i o n was conducted b y a p p ly in g , and l i g h t l y ru b b in g in , one drop (~ O.C... m l) o f a 5Vic and 2% s o lu t io n (w t/vo l) o f the t e s t m a te r ia l in d i s t i l l e d w a te r on th e sh aved i n t a c t sh o u ld e r s k in o f 10 male a lb in o guinea p ig s . A second sent o rf 1iu0 g u in e a p ig s was tir e a twe ud iuni tuhnec Dsaamme maununer u sin g a % and j1-%v c o n c eennttrraattieo n in d i s t i l l e d w ater. To t e s t fo r th e s e n s it iz a t io n p o t e n tia l, a s e r ie s o f fo u r s a c r a l in trad erm al in je c tio n s was g iv en , one wach week over a th re e week p e rio d , which cons:, red o f 0 .1 ml o f a 1.0 $ s o lu tio n (w t/vo l) o f t e s t m a te ria l in d i s t i l l e d w a ter. F o llo w in g a two-week r e s t p e r io d , th e second group o f anim als, i n i t i a l l y t r e a t e d a t % and l'Jo co n cen tration s, were ch allen ged fo r s e n s itiz a tio n by ap p ly in g, and l i g h t l y rubbing in , one drop u. 05 ml) o f a % and 1% s o lu tio n (w t/vol) o f t e s t m a te r ia l in d i s t i l l e d w ater on th e shaved i n t a c t sh ou ld er s k in . A group o f 10 p r e v io u s ly unexposed guinea p ig s r e c e iv e d s im ila r a p p lic a tio n s a t th'~ tim e o f c h a lle n g e t o p ro v id e a d i r e c t coup a r is e n o f th e c h a lle n g e r e a c tio n s oh s k in i f s im ila r a g e. Company Sanitized. Does not contain TSCA Results Concentration in d is t ille d water Primary I r r it a tio n T est 2k Hours h8 Hours Challenge Test 2k Hours k8 Hours Number of animals sensitized** O Reactions* on Intact Guinea Pig Skin (1s t Grp: 1 0 Animals - In. Avg. ht. = 462 g 2nd Grp: 1 0 Animals - In. Avg. Wt. = U68 g F in a l Avg. Wt. = 683 g) F ir s t Group ~W 25$ Second Group 5% 1% . (10 New Anim ais - A verage W eight = 677 g ) % 1 7++, 3+ 10++++ 2++, 6+, 2 neg. 5++++, 1++, U+ 10 neg. 10 neg. 1 +, 9 neg. 1+. 9 neg. O/lO lu neg. 1C neg. 2+, 8 neg. *4. 8 neg. * 1+, 9 neg. 1+, 9 neg. 3+, 7 neg. 1+, 9 neg. - 2- troduced m ild t o stro n g i r r i t a t i o n w ith edema when t e s t e d on g u in e a p ig kin a t 50JL No s e n s it iz a t io n was observed on any o f th e t e s t an im als. Care sh ould b e e x e r c is e d when h a n d lin g s o lu tio n s o f t h i s m a te ria l and any c o n ta c tin g th e sh in sh ould be p ro m p tly washed o f f w ith w a te r .______________________ * R eactio n Code: +, ++, +++ = m ild , m oderate, stron g erythem a; ++++ = erythem a p lu s edema; +++++ = n e c r o s is . ** S e n s itiz a tio n i s d efin ed as a tw o -step sco re in c re a se (++) a t ch a lle n g e over the respon se ex p ected from th e same amount a p p lie d i n i t i a l l y o r on th e co n cu rre n t c o n t r o ls . Company Sanitized. Does not contain TSCA ce. Report by: 0 . Louis D a sh ie ll OLD :pgh May 23, 1773 Approved by: Charles F. Reinhardt A ssistan t D irector