Document By9KYMxjn9RMdwpeEKkkyayxj
AR R26 _ 0699
MICROBICS MICROTOX TOXICITY TEST
TEST SUBSTANCE
Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-fl9u5orooroacstaanecsouimfpoonnaeten,twohfiFcCh-m2a01yAaFl.so(b1-e Octanesulfonic acid) (CAS # 2795-39-3).
RiseFmCa-r2k0s1:AF. TChuerr3eMntpirnofdourcmtaitoinonloitnnduicmabteerswitaiss anmoitxntoutreed.of T3h%e PteFsOtSs,ample 81.82.55%9%Acdriyeltihcyfloenaemegrl,yc9ol%bsutoydlieutmheorc,ty3l7s.u8lf1a%tew,a0te.r3,%1s4o%dieutmhyllauernyel gsluylcfoalt,e, 3% triethanolamine, 6% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture ith incompletely
characterized concentrationsofimpurities. Data may not`accurately reflect the toxicity of the fluorochemical component ofthe test.sample.
METHOD:
Method: Microbics Microtox "BASIC" Procedure
Test type:
GLP: No
Acute static
YSpeeacriCeosm:plPehotteodb:ac1t9e9ri5um phosphoreum
Analytical Exposure
pmeorniiotdo:ri3n0g-:mipnHutaensd
osmotic
condition.
Statistical methods: ECS0 values calculated by statistical linear regression
TpersotgroarmgaprnoivsimdesdouforrcMei:crMoitcoroxbics' Reagent.
Test conditions:
Diluent: Millipore Mili-QTM water
TSetsotckteamnpdertaetsutrseo:luNtoiotnnoptreedp.aration: A 1000 mg/L primary stock
ssoolluuttiioonn wwaass 6p.r8.e"paArnedosinmoMtiillcipaodrjeuMsitlmlein-tQwTMawsatmera.deThuesiPngHo2f0t0hmegstock
NwaaCslndoitsesdolavsed"cilnea1r0amndL proportional dilutions.
csotloocrklessosl"t.uioAnl.l
teAsptpseoalurtainocnseowfetreestmasodleutbioyns
Exposure vessels: 4 mL cuvettes.
Number of replicates: two
Number of concentrations: Four plus a blank control Element basis: Percent light loss
RESULTS
Nominal concentrations: Blank control, 62.5, 125, 250, and 500 mg/L
000301
Element value: ~~ 5-minute ECso = 154 (141 -- 167) mg/L
15-minute 30-minute
EECCssoo
==
8683
((6817
--
6884))
mmgg//LL
Element values based on nominal concentrations.
Remarks: Substance
RTeesmtairnkgswafiseldc. onTdhuectveadluoens
a mixture reported
aapspldyestcortihbaetdmiinxttuhreeTaensdt
not
the fluorochemical proportion alone.
LUSIONS
Tdehteertemsitnseudbtsotbanec6e33m0g-/mLi,nuwtiethEaC9s5f%orcPohnoftiodbeanccteeirnituermvpalhoofsp61hotroe6u4mmwga/sL..
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, MN, 55133
DATA QUALITY.
Reliability: Kiimisch ranking 3. Data sheets for the 30-minute exposure csotuulddy nlaoctkbseanlaolcyattiecda.l cSoanmfiprlmeatpiuorniotyftwhaes anomtoupnrotpeorflfylucohraorcahcetmeirciazledparonpdortthieon
in the solution.
REFERENCES TMhiinsnesstoutday,wLaasbcRoenqduuecsttednubmybtehre N32M9E6n4vi1r9o95n.mental Laboratory, St. Paul, OTHER Last changed: 6/26/00.
000302
Ub/25/2000
ENVIRONMENTAL LABORAIURY
1
ARCHIVED FINAL REPORT
LAB REQUEST NO. N2964 REQUESTOR NAME: SBS/SHARILYN LOUSHIN DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 08/24/1995 DESC: FC-201AF (L-13652)
CONTRACT LAB(S): EXP COMP DATE: 11/03/1995 DATE COMPLETED: 12/02/1995 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176
# 07-2573-9.
Results From Activated Sludge Respiration Inhibition Test:
Test Conc. ng/1 1000 1000 (dup) EC50 sab 9/20/95
Percent Inhibition in Respiration Rate
30-min Exp.
3-hr Exp.
0
0
0
0
> 1000
> 1000
BOD and COD will be on a separate lab request.
sab 9/20/95
-
Results from 48-hour Daphnia magna Toxicity Test: No immobilization was seen after 48-hour exposure to 1000 mg/L. sab 9/28/95
Toxicity found on
to the freshwater green lab request N3281.
algae,
Selenastrum
capricornutum
can
be
sab 10/3/95
Results from Test Conc. mg/L 62.5 125 250 500 EC50 95% CI GJ 12/01/95
Microtox Test: Percent
5-Min Exp. 24 46 64 76 154
(141-167)
Light Loss 15-Min Exp. 37 64 78 86 88 (87-88)
30-Min Exp. 50 76 86 92 63
(61-64)
Results from Test was run
96-hr Fathead Minnow as a static-renewal.
Toxicity Test: Beakers were renewed
at
48
and
72-hrs
of
exposure. This was done because the required 60% of saturation.
the GWJ
dissolved 12/01/95.
oxygen
levels
dropped
below
Test Conc.
Percent Mortality
mg/L 100
24-hrs exp. 0
48-hrs exp. 0
72-hrs exp. 5
96-hrs exp. 5
180 320
0
5
5
5
30
35
10 45
560 1000
100
100
100
100
100
100
100
100
LC50
410
350
345
310
95% CI % Trin
(390-435) 0
(305-395) 0
(300-400) 5
(270-370) 5
Trimmed Spearman-Karber Statistical Program was use to calculate LCS0's.
000303
J 12/01/95.
SAMPLE DATE CODE
DESCRIPTION RESULT
MDL or (95% C.1.)
06/25/2000
LAB REQUEST NO. N2964 SAMPLE DATE CODE
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
CONTINUED DESCRIPTION RESULT
2 MDL or (95% C.1.)
1
FC-201AF or Green liq
48-HR-EC50 96-HR-LCS0
DAPHNIA ACUTE TEST FISH ACUTE TEST - 96 HOUR
3>110000MG/MLo/L
MICROTOX MICROTOX - EC50
63 Ma/L
SLUDG-RESP SLUDGE RESPIRATION - EC50 >1000 MG/L ~~
)(270 - 370) (61 - 64) (-)
000304
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