Document Br04gjL84JGZjYbxXwGrjVX
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424
TRADE SECRET
ARR26 -- 324-2
Dupont10249
Study Title
H-25240: Corrositex In Vitro Test
Laboratory Project ID: DuPont-10249
AuTHOR: Carol Finlay, B.A.
STUDY COMPLETED ON: April 10, 2002
PERFORMING LABORATORY: E.L du Pont de Nemours and Company
Haskell Laboratory for Elkton Road, P.O. Box
Health 50
and
Environmental
Sciences
Newark, Delaware 19714-0050
Wor Request Nuvser [SN]
service Cove NuvsexJIN]
Paros
od.Doesnotcontain Tscacal
1.25240: Comositex In Vitro Test
DuPont 10249
CERTIFICATION `We, the undersigned, declare that this report provides an accurate evaluation of data obtained from this study.
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IssuedbyStudyDirector: LA oyf.* ay SuffScent
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Company Sanitized. Does not contain TscA cam
ET
--
H-25240: Corrositex In Vitro Test
STUDY INFORMATION
Synonyms/Codes:
|
+ H25200 Haskell Number: 25240
CAS Registry Number fr)
DuPont-10249
} |iE
Physical Characteri[ stics: --) Sponsor: WELi.lmdiungPioonnt,dDeeNleamwaoruers19a5n9d3Company
USA.
Study Initiated/Completed: February 26, 2002 / (sce report cover page)
In-LifeIniated/Completed: April 5, 2002 / April 5, 2002
_ -
CompanySanized. Does not contain TSCA 054
1.25240: Comositex In Vitro Test
STUDY PERSONNEL Study Director: Carol Finlay, B.A. Management: Scott E. Loveless, Ph.D. Primary Technician: James C. Mackay II `Toxicology Report Preparation: Lisa G. Burchfield, A.A. Management: NancyS. Selzer, M.S.
Dupon-10249
CompanySenitized.Does notcontain TSCA Cai t TT et
125240: Comositex In Vitro Test
DuPont10249
SUMMARY
'H-25240 was evaluated for skin corrosion potential using the In Vitro International Corrositex
assay.
based
oCnorthreostiitmeexreiqsuairsetdanfdoarrtdhiezetdestansdubqsutaanntcietattoivpeasisn
tvihtrrooucgohrraosbiivoibtayrrtieesrt.meTmhbermaondeelanids
produce a change in a chemical detection system.
An did
aliquotof500 4L not pass through
of H-25240 was applied anyof the membranes.
to
each
of4
membrane
discs.
The
test
substance
Under the conditionsofthis test, H-25240 was nota corrosive substance.
=
`CompanySanitized. DoosnotcontTSaCAiCn2
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eee
125240; Comostexn Vir Tet
Dupont 10249
INTRODUCTION The purpose of this study was to determine skin corrosion potential of H-25240 using the
In Vitro Intemational CorrositexTM assay kit.
MATERIALS AND METHODS
A. Test Substance ""TThheetteesstt ssuubbssttaannccee,aHp-p2e5a2r4e0d,towabse sstuapbpleliudndbeyrtthheescpoonndsiotirA oanss of-- the s-- tudy.--No e-- viden-- ce 0 instability, such as a change in color or physica sate, was observed.
B. Principles of the Method
"CTohreroassistaeyxis ibsaasnedinonvittrhoe aasbislaiytyuosefd tcoordreotseirvmeitneestthseubssktiannccoertroospiaosnsptohtreontuigahlaobaiotf beasrtriseurbstance.
p`rmoepmrbiretaanreyabnidobtaorrciaeursmeeamcborlaonrechcaonnsgiestisnotfhalrieqcuoindsCthiteumtiecdaclolDleatgeecntimoinxtSuyrse.teTmh(eCDCSD).S iTshe
~
composed ofwaterand pH indicator dyes. Corrositex is conducted in three steps:
I. Qualification Step
p"Tlhaecetdestdisruecbtsltyanicnetoisapvriea-lqcuoanlitfaiiendintgotehsetaCbDliSs.h cIofmpaacthiabinlgietyinwciotlhotrhoeracssoanys.istTehnecyteosctcsuurbssttahncteesits substance can be tested by using this asay.
2. Categorization Step
s"TuhbisstsatnecpeiestpabllaicsehdesintthoeVciaatlegAor(ycoofnttahienitnegstasnubasitdanbcuefffeorr)uasnediVnitahleBcl(ascsoinftiaciantiinogn astebpa.seThbueffeesrt), 0a:n2d cthoelovriaclhsaratr.e Tobhseertveetdsufborstaaoncleoriscahsainggen.edAtoccoiltohrecrhCaantgeegoorbyse1rovredCaitneegiotrheyr2vibaulsiesdmoanicthheed ccloalsosrifcihcaantigoenisnteepa.ch vial. Category | and Categor2y are indicativeofthe time scales used in the
3. Classification Step
r"Tehgiuslasttieopndseatnedrmcilnaesssiiffitchateiotness.t sTuhbesttaensctesuisbstsaknicneciosrarpopsliiveedaocncotrodpinogftthoeUpNroaprnidetDaOryTbiobarrier tmheamtbtrhaentee.st sAubcsotlaonrccehhaansgepaosrsecdontshirsotuegnhcythcehamnegmebriannteh.e CTDhSe bdeenteeartmhintahteiomneomfbrcoamnreosiinvdei/cnaotne.s
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HL25240: Comositex In iro Test
DuPont10249
--
comosive substance
and the to pass
Packing through
Group assigned are the membrane. The
based on the amountof time i time scales differ for Category
takes 1 and
the 2.
test
v"iTahlescdaetteegrormyinaesstihgenpeadcaknidngthgeromuep.anTvhaelufeoolfltohwiengbrteaablkethirsouusgehdttiomaessfoirgnaltlh4e saappmrpolperiraetpeliPcaacteking Group by category and breakthrough time.
PACKING GROUP DESIGNATION
Corrositex Times
[Category2 |0to3minutes|>3to30minutes| > 30 to 60 minutes
Packing Group I | Packing Group Ill |Non-Corrosiv|e
> = greater han
C. Protocol
_ (`T2h-e8bCio)buanrtriilerthmeebmibobraarnrieerdimsecsmbprroavniedsedwebryetphreempaarneudf.acTtuhreerbiwoebarrerisetrormeedmibnraanreefsriwgeerraetoprrepared
tthhaetdwaaysprpiloarcetdo coonnaduhcottipnlgatthee/maasgsnaey.ticAsttihrreerrm.oTmehteewrawtaersbiantsherctoendtianitnoerawwaastefrilblaetdhwciotnhtainer
approximately 1 inchof water and heated (0 68-70C. While the water bath was warming, the
cmoenmdebnrsaantieond.iscTshweereentrireemcoovnteedntfsroofmtthhee rbeiforbiagerrraiteorr.dilTuheenttwreayrelidadwdaesdrteomtohveevdiatloo fprbeivoebnatrrier
mmaetdriixumposwpdeeerd. unTthiletvhiealbiwoabasrrtiheern mpaltarciexd pionswiddeerthweaswactoemrpblaetht.elyTdhiessmoilxvteudre(awpapsrosxtiimrraetdealty
20 minutes). 5 minutes to
The stir knob and heat were allow any air bubbles to rise
turned off. The to the surface.
solution
remained
in
the
water
bath
for
w`Tawsochouvnedrreeddamnidcrnooliatrerbsubwbalsepsipheatdtefdorimnetod.eaTchhemefimlblerdatnreaycdoinsct,aiennisnugritnhgethbeioebnatrirrieermsewmabsrane wrapped with plastic wrap and immediately stored in a refrigerator (2-8C).
1 Qualification
`The test substance was first tested to determine ifthe sample was compatible with the sChoarkreons,itaenxd asylsltoewme.d tAo s1t5a0ndufLoral|iqmuiontuotfet.heAtceshtasnugbesitnanccoelowraosr acdodnseidsttoentchye wQauasloibfsyetrevstedtube,
qualifying the test substance for use in the Corrositex system.
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Company aniized. Doce! contain TSCA C81
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-
2. Categorization
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A(co1n5t0auiLnianalgiqbuaostoefbufHf-e2r)5.24T0hewavsiaalsddweedretocVaipapledAa(ncdonsthaaikneinn.g aNnoacciodlobrufcfhearn)gaenwdaVsiaolbsBerved. T`mwixoedd.roTphsoefcoCloonrfwiarsm mRaetacgheendt wtoertheeacdodleodrstoonVitahle Bc.oloTrhcehavritasl. wTahsecacpoploerdcahnadngsehapkleanceudnttihle sample in Category 2.
3. Classification
`tThheebfiiolblaerdrtirearymcaotnrtiaxinwiansg ptlhaecbeidobianrtroiearcshweamiscpaultdoentteoctcirounsshyesdtiecme.(CADSm)emvibarl.anTehdeisCcDcSonvtiaailnsing
were at room temperature (17-25C). evenly applied on topofthe disc and
Within 2 minutes, a timer was started.
an aliquot of 500 uL This procedure was
of H-25240 was followed for each
o(fvitahle l4abteesltedvi"al+s)(vcioanltsai1n-4i)n.g Osunlefurviicalawciads] sainmdilaanroltyhterreawtaesd wsiimtihl5ar0l0yutrLeaotefad wpiotshit5iv0e0cuoLntroofla
cnoelgoarticvoentcrooln.troTlh[e(vviiaallslawbeelreedt"h-e"n) coobnstearivneidnfgocritaricchaacnidg]e. inAthveiaCl DlSab.elTedheCtsiemreveitdtaosoka fCoDrSthe
positive control to break through the membrane was documented.
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`Company Sanitized. Doos notcontain TSCA CGI 5
hH-o25s240e:CCoorvmosioteexiInntViitrroo TTeesst
RESULTS Breakthroughofthe biobarrier occurred as follows (hour:minute:second):
Vial 1 (Test Substance) - No breakthrough Vial 2 (Test Substance) - No breakthrough Vial 3 (Test Substance) - No breakthrough Vial 4 (Test Substance) - No breakthrough
Vial 5 (Positive Control) - 00:01:11 Vial 6 (Negative Control) - No breakthrough
CONCLUSION Under the conditionsofthis test, H-25240 was not a corrosive substance.
oDuwPoenwt-i1o02s4s9
RECORDS AND SAMPLE STORAGE
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Specimens(ifapplicable), raw data, and the final report will be retained at Haskell Laboratory,
Newark, Delaware, or at Iron Mountain Records Management, Wilmington, Delaware.
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