Document BjN4qvV6BZN1Vy57Ym4ykb9k
AR226-2965
DU FONT USE ONLY
Du Font HLR 636-91
Study Title
STATIC, ACUTE, 96-HOUR LC50 oqfi--------|^
TO FATHEAD MINNOWS ( PIHEPHALES^SDHELAS)
Author Kevin N. Baer, Ph.D.
Study Completed On September 24, 1991
Performing Laboratory
Haskell
E. I. du Font de Nemours and Company
Laboratory for Toxicology and Industrial Elkton Road, Post Office Box 50
Newark, Delaware 19714
Medicine
Laboratory Project ID Haskell Laboratory Report Ho. 636-91
Page 1 of 12
company Sanitized. Does no! contafn rsr.A CB?
Du Pont BLR 636-91
TABLE OF CONTESTS
Page
Table of Contents
2
.......................... I. Summary
3
............................. II. General Information
4
....................... III. Purpose
6
............................. IV. Records
6
............................. V. Materials and Methods
7
A. Test Materials
7
...................... B. Dilution Water
7
....................... C. Animal Culture
7
....................... D. Test Methods
7
....................... E. Chemical Analysis
8
........................ F. Statistical Methods
8
........................................... VI. Results and Discussion
8
..................... VII. Conclusion
8
........................... VIII. Literature Cited.
8
........................ IX. Tables
9
1. Chemical Characteristics of Haskell Laboratory Well Water
.
.
9
............................. 2. Water Chemistry of the Dilution Water atJTest Start.
10
3. Physical and Chemical Characteristics of|^------ttKTest
.^^^^^.. ..... Solutions.
11
4. Observed Mortalityof Fathead Minnows (Pimephales promelas)
. . . . . . . . . . . . . . ..... Exposed tof--------Eor 96 Hours in a Static, Unaerated Test . 12
'"SsRifeeii Dees not contain TSCA C"
Du Font HLR 636-91
I. SIMHaR? |f^--^--__jkras moderately acutely toxic to juvenile, fathead minnows
(Pimephales promelas) in a 96-hour, unaerated, static test using nominal test concentrations of 6.3, 13, 25, 50, and 100 ag/L. The 96-hour LC50 was 21 mg/L with a 95% confidence interval of 16 to 29 mg/L.
Work
Technician
^ j ^ ^ y ^ i ^ ^ RSoobeerrct WW..'PPeeterson
Technician
/^</^ Study Directors
/!/. ^cm^
Kevin BN. Baer, Ph.D.
Research Toxicologist
Environmental Sciences
y ^ / ? / /Day/Year
^ V A / Mo/Da^Year
y/w^/
Mo/Day/year
THIS REPORT IS APPROVED FOR ISSUE.
, / ^ /^.-A^ Kevin N. Baer, Ph.D.
Study Director
?/^h,
Mo/Day/Year
II. GEMKML BTOEWHM
Material Testeds Hedieal Besearch Numbers
Baskell fluinber: Haskell Laboratory Test Codes
CAS Registry NUBber;
18,920
Du Font Hm 636-91
Sponsor:
Du Pont Chemicals
E. I. du Pont de Memours and Company
yilmington, Delaware 19898
'Company "Sa'nitize'a. Does nol contain TSCA CW
Du Font HLR 636-91
Submitted
Bys]
-Thi Font Chemicals Jackson Laboratory
Deepvater^ KJ
Study Schedule:
Study Initiation Dates 8/30/91 Experimental Start Date: 9/5/91 Experimental Termination Dates 9/9/91
Study Completion Dates 9/24/91
Notebookss
Distribution;
Voesww
TSCA CBI
Du Font HLR 636-91
III. PURPOSE
The goal of this study vas to assess the acute toxicity of juvenile, fathead minnows (Piaephales promelas).
IV. JuKXXBDS
All data and the final report vill be maintained either at Baskell
Laboratory for Toxicol-jgy and Industrial Medicine (Newark, Delaware) or be archived at the Du Font Records Management Center (Vilmington, Delaware).
^
i.^.s-""
Du Font HLR 636-91
V. K&TERIALS AND HETHODS
A. Test Materials
^HHH^s Source and composition of the test substance are described under General
Information. The appropriate quantity
measured and dispensed
into 4-L of dilution water (see frplfflr) toyrepareeach test concentration. Stability and solubility o|H--------in dilution water were not determined.
<^^^^3P
B. Dilution ffater
water (Table 1) originated from the Haskell Laboratory veil.
C. Animal Culture
Fathead minnows (Pimephales promelas) were reared in continuously flowing well water at Haskell Laboratory. Adult fish reside in 38 L aquaria at approximately 25C and were fed frozen brine shrimp (Artemia sp., Golden Gate
Brand, Korden Division, Novaiek, Inc., Bayward, CA) generally twice daily,
once on Saturday, and not on Sundays. Eggs were deposited on polyvinyl
chloride tiles coated with sand. Tiles with eggs were aerated and incubated
at approximately 25C in a water bath. Larvae were reared in 22 L aquaria at approximately 22 C and were fed newly hatched Artemia generally twice daily on weekdays and daily on weekends. Juveniles were held in a 22 L aquaria at approximately 22C and were fed newly hatched Artemia and/or frozen brine
shrimp generally twice daily on weekdays and once on Saturday and Sundays. No treatment of the fish for diseases was required during the holding period. Sickness, injury, and abnormality were not observed. Animals were identified by labels on aquaria and test vessels.
D. Test Methods [11
Five test concentrations and a water control were used. Nominal concentrations were 6.3, 13, 25, 50, and 100 mg/L. The fathead minnows ranged from 1.3 to 2.0 cm in standard length (mean 1.5 cm) and 0.024 to 0.096 g in wet weight (mean 0.044 g). The fathead minnows '-e 51 days old
at test start.
Glass beakers (4+-L) containing 4-L of test solution (approximately 13-cai liquid depth) were employed. One replicate of each test concentration with ten fish each were used. Fish were added to the test concentrations using random numbers and were not fed for 48 hours prior to the test nor during the test. Mean loading for controls at test conclusion was 0.11 g/L. Test solutions were held between 21.0 and 21.8C (mean 21.4C) and were unaerated. A photoperiod of 16 hours light (129-183 Lux) versus 8 hours darkness was
employed.
Dissolved oxygen (YSI Model 54A, Yellow Springs Instruments, Yellow
Springs, OH) and pH (Corning Model 610A, Corning Scientific Instruments,
Company Sanitized. Does noi contain TSCA CBI
"u Font BLR 636-91
Kedfield, MA) were measured in the control and all test concentrations at the
beginning of the test (before the addition of fish) and daily thereafter. Temperature (American Society of Testing Materials registered mercury stem thermometer) vas measured in the dilution water control at the beginning of
the test and dally thereafter. Total alkalinity (Hach kit. Bach Chemical Company, Loveland, CO), EDTA hardness (Bach kit. Bach Chemical Company,
Loveland, CO), and conductivity (YSI Model 33. Yellow Springs Instruments,
Yellow Springs, OH) of the dilution water were measured at test start.
E. Chemical Analysis Chemical analysis of the test substance in the test solutions was not
conducted.
F. Statistical Methods The 96-hour LC50 and its associated confidence interval was calculated
using a moving average-angle program [2]. VI. RESULTS AND DISCUSSION
NominalUH--Aconcentrations used in the definitive test were 6.3, 13,
25, 50, and 100 mg/L.
All chemical and physical parameters were within expected ranges (Table 2 & 3). The 96-hour LC50 was 21 rag/L with a 95% confidence interval of 16 to 29
rag/L.
VII. CONCLUSION
iil------was moderately acutely toxic to juvenile, fathead minnows
(Pimephales^romelas) in a 96-hour, unaerated, static test using nominal test concentrations of 6.3, 13, 25, 50, and 100 mg/L.
VIII. LITERATURE CITED
1. Zucker, E. 1985. Hazard Evaluation Division, Standard Evaluation Procedures Acute Toxicity Test for Freshwater Fish. EPA-54019-85-006. United States Environmental Protection Agency, Office of Pesticide Programs,
Washington, DC 20460.
2. Peltier, V.H. and C.I. Weber, Eds. 1985. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Organisms. EPA/600/4-85/013.
United States Environmental Protection Agency, Environmental Monitoring and Support Laboratory, Cincinnati, OB.
p^s^ea.^^^^TM'^'
TABLE 1
Du Font HLR 636-91
Chemical Characteristics cf Haskell Laboratory Veil Water1
Parameter
Concentration
parameter
Concentration
BOD', Hg/L
600, ng/L DOC3, Bg/L
TOC4. mg/L
Kjeldahl N. rog/L
Alamonia N, mg/L
Turbidity, NtU Phenolics, mg/L Color, Co/Pt Solids
total suspended,
Aluminum, mg/L
Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Boron5, mg/L Cadmium, mg/L
Calcium, mg/L Chloride6, mg/L
Chromium, mg/L
Cobalt, mg/L Copper, mg/L Cyanide, mg/L
Iron6, mg/L Fluorlde6, mg/L
<2
13
1.5 <0.5 0.2 <0.05 <1.0 <0.050
<5
mg/L
<2
<0.100 <0.060 <0.010 <0.001 <0.020 <0.002 25.0
6.3
<0.010 <0.020 <0.010 <0.025 0.053 <0.1
Lead5, mg/L
Magnesium, mg/L HBAS7LAS. mg/L
Mercury, mg/L
Nickel, ng/L Nitrite6, Bg/L Nitrate6, mg/L Phosphate6, ng/L
Potassium, mg/L Selenium, mg/L
Silver, mg/L
Sodium, mg/L
Sulfate6, mg/L Sulfide, mg/L Thallium, mg/L
Zinc5, ng/L
Volatile priority pollutants
Acid extractable priority pollutants
Base/neutral
priority pollutants
Pesticides/PCBs
Organophosphate
pesticides7, ug/L
<0.005 3.5
<0.05 <0.00020 <0.020
<0.l 1.6
<0.1 1.8
<0.005 <0.010
8.1 4.8 <0.05 <0.01 <0.020
ND
ND
ND ND8
<0.50
XDate of analyses 17 October 1990 unless indicated otherwise, analyses performed at Environmental Testing and Certification Corporation, Edison, New
Jersey; ^ate of analyses 13 December 1990, analyses analysis performed at Environmental Testing and Certification Corporation, Edison, Nev Jersey; ^ean value; ^ate of analyses 24 July 1990, performed at Du Font Engineering Test
Center, Newark, Delaware; 'Below the Published Method Detection Limit (BMDL);
^ate of analyses 9 November 1990, analyses performed at Du Font Chemicals,
Jackson Laboratory, Oeepwater, New Jersey; '"Date of analyses 23 October 1990,
analyses performed at Hazelton Laboratories, Inc., Madison, VI; 'None detected.
^--"
Du Font HLR 636-91
TABLE 2
Vater Chemistry of the Dilution gater at Test Start
Total Alkalinity
(ffig/L as CaCOs)
81
EDTA Hardness
(ng/L as CaC03>
74
Conductivity
(umhos/cm)
190
CW^B^ed.Ooe.notco^----------
10
Du Pout 9LR 636-91
TABLE 3
Physical and Chemical Characteristics of Test Solutions
Nominal Concentreifcions (nilB/L)
Time
(Hours)
Control
6.3
13
25
50
100
H
0
8.7
8.7
8.7
8.7
8.6
8.6
24
6.2
6.2
6.5
6.4
6.8
7.0
48
6.0
6.1
6.4
6.2
6.5
6.9
72
6.1
6.3
6.2
6.4
6.4
6.5
96
6.7
6.3
6.2
5.9
6.0
6.3
Dissolved Oxygen
0
7.4
24
7.2
48
7.3
72
7.2
96
7.9
7.4
7.5
7.5
7.5
7.5
7.1
7.5
7.5
7.5
7.5
7.2
7.4
7.4
7.4
7.4
7.2
7.3
7.2
7.2
7.2
7.9
7.9
7.9
7.9
7.9
^OtftpahvSanH^es. S^3 ^ ee"^^ '~l^f"!t CBl
11
Observed Mortal!t to
TABLE 4
Du Font HLR 636-91
Fathead Minnows (Pimephales promelas) Exposed
'or 96 Hours An Static, Unaerated Test
Nominal Concentrations
(mg/L)
Control 6.3
13 25 50 100
Obiserved Morta]Lity in Percent
Tine (Hours)
24 Hours
48 Hours
72 Hours
0
0
0
0
0
0
0
0
0
0"
70
80"
20"
100
100
6^
100
100
96 Hours
0 0 0 80c 100
100
Five fish were at the surface, were dark. in coloration, and had an accumulation of an unknown substance on their pectoral fins. ''Four fish were at the surface, were dark in coloration, and had an accumulation of an unknown substance on their pectoral fins. eAll surviving fish were at the surface and had a dark coloration. ^11 .surviving fish exhibited dark coloration.
^t.iii sSCAS&J
12